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Informe De Ensayo Última versión 31 01 2020 CB SUBMAREAL ID único ILS0120_P01 Informe de Ensayo 1. Identificación Cliente Cliente CESMEC S.A. Responsable Sofia Perez Dirección Ramon Freire 50, Colina-Santiago Contacto [email protected] 2. Identificación del método de ensayo Responsable del ensayo ENVIROLAB SpA Método de ensayo Res. Exe. N°3612 Laboratorio de análisis biológicos Desarrollo ensayo Autoriza el ensayo Claudia Aburto Leiva ENVIROLAB SpA 2.1 Características del método de ensayo Previa separación de los organismos del sedimento marino, mediante el uso de un tamiz de 1 mm, posteriormente se identifican todos los organismos encontrados. La identificación se determina al nivel taxonómico más bajo posible y debe realizarse bajo lupa. Además, en el caso de las familias Capitellidae, Oweniidae, Cirratulidae y Nassaridae, es obligatorio que la determinación sea, como mínimo, a nivel de género. Descripción Posteriormente, los organismos se dejan drenar por 10 minutos para eliminar el exceso de alcohol. Luego, se pesan en una balanza analítica, por taxón, para obtener el peso húmedo alcohólico. En este informe de ensayo, la abundancia se expresa en n/m2, mientras que la biomasa va expresada en g/m2. 3. Identificación de los ítems de ensayo Tipo de muestra Sedimento marino submareal Localidad muestreo Bahía de Coronel Tipo de envase Frascos plásticos de 250 cc con contratapa Fecha muestreo 16/01//2020 Ítems de ensayo totales 30 unidades Fecha ingreso al Laboratorio 20/01/2020 Preservante Alcohol al 70% Emisión Informe de Ensayo 29/01/2020 ENVIROLAB SpA – Av. del Valle Norte 937 – Oficina – 258 – Huechuraba – Santiago 1 Última versión 31 01 2020 CB SUBMAREAL ID único ILS0120_P01 Informe de Ensayo 4. Identificación ítems de ensayo ingresados al Laboratorio Número Rótulo Número Rótulo Número Rótulo Número Rótulo 1 E1R1 11 E4R2 21 E7R3 2 E1R2 12 E4R3 22 E8R1 3 E1R3 13 E5R1 23 E8R2 4 E2R1 14 E5R2 24 E8R3 5 E2R2 15 E5R3 25 E9R1 6 E2R3 16 E6R1 26 E9R2 7 E3R1 17 E6R2 27 E9R3 8 E3R2 18 E6R3 28 E10R1 9 E3R3 19 E7R1 29 E10R2 10 E4R1 20 E7R2 30 E10R3 5. Resultados Datos obtenidos por estación de monitoreo, correspondientes a los ítems de ensayo analizados por los profesionales de ENVIROLAB SpA. ENVIROLAB SpA – Av. del Valle Norte 937 – Oficina – 258 – Huechuraba – Santiago 2 Última versión 31 01 2020 CB SUBMAREAL ID único ILS0120_P01 Informe de Ensayo Tabla 1. Resultados de abundancia y biomasa obtenidos en la Estación de Monitoreo Submareal 3. Réplica Phylum Clase Orden Familia Género Taxa Abundancia (n/m2) Biomasa (g/m2) Cancridae Cancer Cancer plebejus 10 60,240 Malacostraca Decapoda R1 Arthropoda Paguridae Pagurus Pagurus sp 10 0,200 Maxillopoda - - - Maxilipoda 10 0,220 Mollusca Gastropoda Neogastropoda Nassariidae Nassarius Nassarius dentifer 20 28,070 R2 Arthropoda Maxillopoda - - - - 30 6,260 Mollusca Gastropoda Neogastropoda Nassariidae Nassarius Nassarius dentifer 30 44,640 Tabla 2. Resultados de abundancia y biomasa obtenidos en la Estación de monitoreo Submareal 5. Réplica Phylum Clase Orden Familia Género Especie Abundancia (n/m2) Biomasa (g/m2) R1 Mollusca Gastropoda Neogastropoda Nassariidae Nassarius Nassarius dentifer 10 16,010 R2 Arthropoda Malacostraca Decapoda - - - 20 0,300 ENVIROLAB SpA – Av. del Valle Norte 937 – Oficina – 258 – Huechuraba – Santiago 3 Última versión 31 01 2020 CB SUBMAREAL ID único ILS0120_P01 Informe de Ensayo Tabla 3. Resultados de abundancia y biomasa obtenidos en la Estación de monitoreo Submareal 7. Réplica Phylum Clase Orden Familia Género Taxa Abundancia (n/m2) Biomasa (g/m2) R1 Mollusca Gastropoda Neogastropoda Nassariidae Nassarius Nassarius dentifer 20 31,330 R2 Mollusca Gastropoda Neogastropoda Nassariidae Nassarius Nassarius dentifer 10 11,730 Tabla 4. Resultados de abundancia y biomasa obtenidos en la Estación de monitoreo Submareal 8. Réplica Phylum Clase Orden Familia Género Taxa Abundancia (n/m2) Biomasa (g/m2) R1 Mollusca Gastropoda Neogastropoda Nassariidae Nassarius Nassarius dentifer 10 19,630 Arthropoda Maxillopoda - - - Maxillopoda 10 5,650 R2 Mollusca Gastropoda Neogastropoda Nassariidae Nassarius Nassarius dentifer 10 14,620 Arthropoda Maxillopoda - - - Maxillopoda 10 1,420 R3 Mollusca Gastropoda Neogastropoda Nassariidae Nassarius Nassarius dentifer 20 30,900 ENVIROLAB SpA – Av. del Valle Norte 937 – Oficina – 258 – Huechuraba – Santiago 4 Última versión 31 01 2020 CB SUBMAREAL ID único ILS0120_P01 Informe de Ensayo Tabla 5. Resultados de abundancia y biomasa obtenidos en la Estación de monitoreo Submareal 10. Réplica Phylum Clase Orden Familia Género Taxa Abundancia (n/m2) Biomasa (g/m2) R1 Arthropoda Malacostraca Decapoda Paguridae Pagurus Pagurus sp 20 0,190 6. Observaciones Condiciones de los ítems de ensayo, además de las observaciones propias del proceso de ensayo realizado por los profesionales de ENVIROLAB SpA. Indicar si hay alguna modificación al método de análisis. 1. Estaciones de monitoreo submareal E4R2, E3R3, E9R1, E6R2, E4R1, E5R3, E2R3, E6R3, E2R2, E10R3, E7R3, E10R2, E6R1, E9R2, E2R1, E1R3, E4R3, E1R1, resultaron ser NEGATIVAS en el análisis. Las muestras E1R2, E9R3 resultaron ser NEGATIVAS en el tamizado. ENVIROLAB SpA – Av. del Valle Norte 937 – Oficina – 258 – Huechuraba – Santiago 5 Última versión 31 01 2020 CB SUBMAREAL ID único ILS0120_P01 Informe de Ensayo 7. Declaración ENVIROLAB SpA: Tratamiento de ítems de ensayo El muestreo asociado a la extracción de muestras fue realizado por profesionales de ENVIROLAB SpA y está sujeto a la Resolución Exenta Nº3612 numeral 28, sin modificaciones sustanciales. Se adjunta a este documento el informe de muestreo IMS0120_P01. Los resultados expuestos en el informe de ensayo ILS0120_P01 son de correspondencia fidedigna a los ítems de ensayo ingresados a las dependencias de ENVIROLAB SpA (n=30), son válidos solo para estas muestras y no presentan información adicional a la observada por los profesionales de ENVIROLAB SpA durante el proceso de análisis. Claudia Aburto Leiva Jefe Laboratorio ENVIROLAB SpA ENVIROLAB SpA – Av. del Valle Norte 937 – Oficina – 258 – Huechuraba – Santiago 6 .
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