ISP Uppsala Universitet Deadline Box 549 IPPS 15 August 2018 SE-751 21 Uppsala, Sweden Fax +46 18 471 3495 IPICS 2 September 2018 UPPSALA• [email protected] UNIVERSITET Grant application for 2019-2021 Resea rch Groups and Scientific Networks Submit this application and enclosures by email attachment with a scanned/photographed copy of the f irst signed page. Read the separate document Guidelines for Enclosures and Budget for more information. Hover your pointer over the blue underlined words in t his document f or specifi c instructions. NOTE! ISP manages personal data (with care and o nly as long as necessary) provided on t his form an d in enclosures in order to honor t he agreem ents wit h you and w ith Sida.

Program Activity code (if settl ed) Chemistry ~ Other 0 Mathematics 0 Physics 0 Applicant (Research group leader/Network coordinator: ti tl e, given name, fa mily na me) DR . JOHN A.M. MAHUGIJA Deputy DR. KESSY F. Kl LU LYA Address Dep artment/unit: CHEMISTRY University/institute: UNIVERSITY OF Street (visiting address): JULIUS K. NYERERE MLIMANI CAMP US P.O Box number: 35061 Post/zip code: City: DAR ES SALAAM Coun try: TA NZA NI A, UNITED REP UBLI C E-ma il address (es): [email protected] c.tz; johnmahugi [email protected] Website: httQs:LLchemist ry.udsm .ac. tz Telephone and telefax Office I Mobile ·· -=t ~ax +255 222410038 +255 719 971 97+ mii:2 ~E'J.i ~9-7l971 +255 222410038 Name of Research Group/Network: CHEMI"TRY [)(! A~TME. rr I ANALYTICAL AND ENVIRONMENTAL CHEI'J IS:filf 'ERS!TY OF DAR-ES-SALAAM ~lox 35061, DAR-ES-S'L At.! ~_a>~ A lilA, EAST- Ai< .ICA City: DAR ES SA LAAM r""' - -· - -- ND Approved by the Departme ~ . ... /DR. KESSY F. KILULYA Date: 02 SEPTEMBER 2018 Sig~tur of Depa rtment/Name in printing: Summar ~ of budget reguest (SEK) 2019 2020 2021 Total Equipment/spare parts/service 215,000 40,000 30,000 285,000 Consumables/literature/field work 70,000 80,000 69,000 219,000 Con ferences/workshops 30,000 30,000 110,000 170,000 r----- Excha nge visi ts by cooperating scientists 27,000 27,000 25,000 79,000 I----- _£_e llowsb_!_es for training: 0 108,000 108,000 216,000 Support to students 30,000 30,000 30,000 90,000 Costs for audit and RG meet ing 10,000 10,000 25,000 45,000 Network (only) administration costs 0 0 0 0 Total 382,000 325,000 397,000 1,104,000 The following enclosures are submitted Yes I No City: DAR ES SALAAM 1) Research pl an/network program Yes Date: 2ND SEPTEMBER 2018 2) App licant's/Deputy's CV Yes 3) Publications/Theses/ Abstracts No 4) Application for improving gender ba lance No Applicant's~ signature 5) Fellowship ap pl ication(s) No 6) Other (Specify: ...... )

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1. Summary of proposed research/network activity (Research plan/Network program) (Full details should be given in Enclosure 1 Please, carefully read and follow guidelines)

a) Give a summary (half a page) of the research plan/network program by briefly describing the planned activity and its justification, and for scientific research clarify the justification in relation to previous research/literature, postulate the hypotheses to be tested/research questions to be addressed, and indicate which methods will be used. Please note that this is a summary of Enclosure 1, Sections a – c. There is an urgent need for training of highly qualified experts in analytical and environmental chemistry to meet the demands in increasing and growing institutions, industries, areas, activities and challenges that require applications of such knowledge and skills to solve the relevant problems. The general objective of the Analytical and Environmental Chemistry Research Group of the Chemistry Department, University of Dar es Salaam is strengthen the capacity for conducting scientific research and postgraduate training in various areas of analytical chemistry and environmental chemistry and apply the findings in solving problems in the societies/communities for development purposes. The following are the specific hypotheses/research questions: (i) How to improve the conditions and techniques for carrying out research and postgraduate training in chemical analyses? (ii) What are the levels of chemical substances (e.g. contaminants) in food, industrial materials and environmental samples (water, soil, sediments, biota and air)? (iii) How to convert bio-waste into useful products that can be used in various applications such as synthesis of alcohol and adsorbents? (iv) How to improve production and dissemination of high quality research results? (v) How to promote the use of research results and outcomes by the society (communities, industries, etc) and for development? (vi) How to attract sustainable financial support from various sources to ensure long-term stability?

The Analytical and Environmental Chemistry Research Group has the following specific objectives: (i) To improve the conditions and techniques for carrying out research and postgraduate training in chemical analyses. (ii) To carry out chemical analyses of food, industrial materials and environmental samples (water, soil, sediments, biota and air). (iii) To develop capacity in converting bio-waste into useful products that can be used in various applications such as synthesis of alcohol, natural gas purification, development of catalyst and adsorbents. (iv) To improve production and dissemination of high quality research results. (v) To promote the use of research results and outcomes by the society (communities, industries, etc) and for development. (vi) To attract sustainable financial support from various sources to ensure long-term stability.

The research plans to answer these hypotheses/research questions through improved research training, experimentation, financial support, knowledge exchange, dissemination and use of results.

b) Give an overview of objectives, planned outputs and expected outcomes Provide a summary of objectives, planned outputs and expected outcomes (and, if found convenient, formalized in the Logical Framework Matrix). Provide your answers under the below headings. This is a summary of Enclosure 1, Section d.

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Overall objective (impact) and relevance of the activity:

The general objective of the Analytical and Environmental Chemistry Research Group of the Chemistry Department, University of Dar es Salaam is strengthen the capacity for conducting scientific research and postgraduate training in various areas of analytical chemistry and environmental chemistry and apply the findings in solving problems in the societies/communities for development purposes.

This activity is in line with the Tanzania’s Vision 2025, the National Research and Development Policy (2010) and the University of Dar es Salaam research policy (2015), and the Swedish Strategy for research cooperation and research in development cooperation 2015–2021.

Specific objectives for the granting period:

(i) To improve the conditions and techniques for carrying out research and postgraduate training in chemical analyses. (ii) To carry out chemical analyses of food, industrial materials and environmental samples (water, soil, sediments, biota and air). (iii) To develop capacity in converting bio-waste into useful products that can be used in various applications such as synthesis of alcohol, natural gas purification, development of catalyst and adsorbents. (iv) To improve production and dissemination of high quality research results. (v) To promote the use of research results and outcomes by the society (communities, industries, etc) and for development. (vi) To attract sustainable financial support from various sources to ensure long-term stability.

Expected outcomes of the activity:

- Scientific Results: Understanding the properties and processes involving chemical substances in samples and in the environment 1 PhD and 6 MSc students trained/supported high quality research results - Graduations: 1 PhD and 6 MSc graduations - Dissemination (publications/conference contributions, etc.): 7 publications in international journals, conducting 5 contributions to conferences and organizing 1 conference - Scientific exchange: 3 scientific exchanges - Interactions with government and society (outreach): Several meetings and discussions

- Other: Purchasing sample preparation equipment, purchasing consumables (standards, solvents, reagents, etc)

c) Give a summary of the postgraduate education plan Give a maximum half a page summary of the proposed activity with emphasis on the education plan. This is a summary of Enclosure 1, Section e.

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Partial support to meet costs for consumables, literature, field works, instrumental analysis and participating in conferences. At least one PhD fellowship to a relevant institution/laboratory in Sweden or other country in Europe (up to 3 months).

d) Give a summary of the gender perspective on the research and education plans Summarize the information given on gender balance as well as strategies and measures to achieve gender balance given in Enclosure 1, section f. Provide your answers under the below headings

Comment on the current gender balance in perspective of previous years efforts: The current gender balance in the research group is generally reasonable as the females and males represent 40% and 60% respectively. In previous years, the number of females was very low.

Reasons for current gender distribution: Most female students do not study science subjects in Tanzania due to historical perspectives.

Planned strategy to achieve gender balance (if not already the case): To motivate females to study chemistry

Concrete measures to achieve gender balance: Special support to female students and staff; conduct seminars directed to females.

e) Provide a number of keywords

Keywords: Chemical analysis, food analysis, environmental samples, water, soil, sediment, biota, air, analytes, organic chemicals, inorganic chemicals, pesticides, heavy metals, aflatoxins, GC-MS, HPLC, LC-MS- MS, AAS, environmental analysis, support, conference, exchange, female, support

f) Give an overview (half a page) on the background and the development of the research group/network from the start of the ISP collaboration until present time. Please, include improvements/challenges in capacity building of higher education and research, and the needs to further develop (Please note that this is a summary of Enclosure 1, Section k)

The Analytical and Environmental Chemistry Research Group at the Chemistry Department, UDSM was formed about five years ago after merging the analytical chemistry and environmental chemistry groups that previously existed in order to consolidate the efforts and improve research capacity as well as address the numerous challenges that were faced by the two groups.

The environmental chemistry group was formerly supported by the ISP (TAN: 02) in 2004 to 2008. This year, the Director, ISP invited the Chemistry Department to submit an application for consideration for the 2019-2021 grants. Hence, this grant application is submitted in response to that call. The research group at the Chemistry Department is composed of competent and capable staff that are ready to undertake the research activities. The qualified students to be engaged in the proposed activity are available. There is great need for further training as there are currently many institutions in the country that need chemists. The department has most of the equipment needed for conducting the activities. However, some equipment are defective or are missing and therefore some minor equipment and other facilities will be required to be purchased in order to successfully carry out the proposed activity.

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3. Specifications of costs 2019-2021 (Please, carefully read and follow guidelines for Budget, and, if applicable, conditions and instructions for Research Groups’ overhead costs)

3a) Specification of costs in 2019

Specification of Costs in 2019 Costs (SEK) Total (SEK) Equipment/spare parts/service Rotary evaporator (Buchi) with water bath for removal of solvents from 90,000 extracts Vacuum pump (Buchi)-Accessory to the rotary evaporator for vacuum creation 60,000 Chiller circulator-Accessory to the rotary evaporator for 65,000 condensation 215,000 Consumables/literature/field work Chemicals (standards, solvents, reagents and 30,000 chromatographic materials, e.g. adsorbents, columns) Costs for fieldwork 40,000 70,000 Conferences/workshops a) To be visited Support to 2 scientists to attend and participate in 30,000 conference/symposium/workshop where they present papers (oral or poster); Subsistence allowance (up to three days); Travel costs (return air fare/ticket, economy class, Local travel) b) To be arranged 0 30,000 Exchange visits by cooperating scientists Support for 1 exchange visit of cooperating 12,000 Scientists, in both directions e.g. visits for collaborative work, planning, reporting, lecturing etc., under South-South and North-South cooperation. Subsistence allowance (meals, accommodation and incidentals) Travel costs (return air fare/ticket, economy class, 15,000 Local travel) 27,000 Fellowships for training/exchange 0 0 a) North-South

b) South-South

Support to students Partial support for instrumental analysis (e.g. GC-MS) 30,000 Costs at local laboratory or another laboratory in the region. Priority will be given to female students and self-sponsored students (who do not have scholarships) 30,000 Activities for improving gender balance 0 0

Network administrative costs 0 0

Audit costs 10,000 10,000

TOTAL 382,000

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3b) Specifications of costs in 2020

Specification of Costs in 2020 Costs (SEK) Total (SEK) Equipment/spare parts/service Refrigerator with deep freezer 10,000 Computer, scanner, spare parts and servicing of 20,000 analytical equipment/instruments Solid phase extraction (SPE) columns (glass) 10,000 40,000 Consumables/literature/field work Chemicals (standards, solvents, reagents and 30,000 chromatographic materials, e.g. adsorbents, columns) Costs for publication of results including for meetings 9,000 with communities and media Costs for fieldwork 41,000 80,000 Conferences/workshops c) To be visited Support to 2 scientists to attend and participate in 30,000 conference/symposium/workshop where they present papers (oral or poster); Subsistence allowance (up to three days); Travel costs (return air fare/ticket, economy class; Local travel) d) To be arranged 0 30,000 Exchange visits by cooperating scientists Support for 1 exchange visit of cooperating Scientists, in both 12,000 directions e.g. visits for collaborative work, planning, reporting, lecturing etc., under South-South and North-South cooperation; Subsistence allowance (meals, accommodation and incidentals) Travel costs (return air fare/ticket, economy class, 15,000 Local travel) 27,000 Fellowships for training/exchange c) North-South Fellowship subsistence allowance, 1 PhD student (incl. insurance) for 3 42,000 months Accommodation for 3 months 18,000 Bench/supervision fee to host laboratories 30,000 Local travel 3,000 Traveling (air fare, economy class, return ticket) 15,000 d) South-South 0 108,000 Support to students Partial support for instrumental analysis (e.g. GC-MS) 30,000 Costs at local laboratory or another laboratory in the region. 30,000 Activities for improving gender balance 0 0

Network administrative costs 0 0

Audit costs 10,000 10,000

TOTAL 325,000

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3c) Specifications of costs in 2021

Specification of Costs in 2021 Costs (SEK) Total (SEK) Equipment/spare parts/service Gel Permeation Chromatograph (GPC) and accessories 10,000 Spare parts and servicing of 20,000 analytical equipment/instruments 30,000 Consumables/literature/field work Chemicals (standards, solvents, reagents and 20,000 chromatographic materials, e.g. adsorbents, columns) Costs for publication of results including for meetings 9,000 with communities and media Costs for fieldwork 40,000 69,000 Conferences/workshops e) To be visited Support to 1 scientists to attend and participate in conference/ symposium/ 20,000 workshop where they present papers (oral or poster); Subsistence allowance (up to three days); Travel costs (return air fare/ticket, economy class, Local travel)

f) To be arranged Analytical and environmental chemistry conference/ 90,000 110,000 workshop

Exchange visits by cooperating scientists Support for 1 exchange visit between cooperating Scientists, in both directions e.g. 10,000 visits for collaborative work, planning, reporting, lecturing etc., under South-South and North-South cooperation; Subsistence allowance (meals, accommodation and incidentals) Travel costs (return air fare/ticket, economy class, 15,000 Local travel) 25,000 Fellowships for training/exchange e) North-South 0 Fellowship subsistence allowance, 1 PhD student (incl. insurance) for 3 months 42,000 Accommodation for 1 PhD student for 3 months 18,000 Bench/supervision fee to host laboratories 30,000 Local travel 3,000 Traveling (air fare, economy class, return ticket) 15,000 f) South-South 0 108,000 Support to students Partial support for instrumental analysis (e.g. GC-MS) costs at local 30,000 laboratory or another laboratory in the region. Priority will be given to female students and self-sponsored students (who do not have scholarships) 30,000 Activities for improving gender balance 0 0

Network administrative costs 0 0

Audit costs 10,000 10,000

Reference group meeting participation costs 15,000 15,000

TOTAL 397,000

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3d. Justification of budget items

List each budget item, and write a motivation to justify the need and the cost.

BUDGET ITEM JUSTIFICATION BUDGET (SEK) 2019 2020 2021 Equipment/ Spare These items are separately required in sample 215,000 40,000 30,000 parts, services preparation and storage (during extraction, clean-up Rotary evaporator and and analysis), data processing and reporting. The accessories, refrigerator spare parts and services are important in order to with deep freezer, ensure proper functioning of the analytical equipment. computer, scanner, spare Currently, the following equipment/tools are either parts and servicing of defective or missing in the laboratory: rotary analytical equipment/ evaporator and accessories, refrigerator/deep instruments, SPE freezer, scanner, GPC and SPE columns and columns, Gel Permeation therefore need to be purchased or repaired. Chromatograph (GPC), Spare parts and servicing of analytical equipment/instruments Consumables/ Chemicals such as reference standards, solvents, 70,000 80,000 69,000 Literature/ Filed work reagents and other consumables are required for Purchase of standards sample processing, analysis, identification and and other chemicals for quantification of analytes. laboratory works; Literature is important in order to help Publication of results; students and researchers to access useful scientific Field works work. Publications of results will involve costs such as journal fees and for preparation of the manuscripts. Fieldwork funds are for researchers to collect samples and for meetings with societies/communities. Conferences/ Conferences enable researchers to disseminate 30,000 30,000 110,000 workshops their research findings to other scientists as well as Partial support to share experience from other scientists/exchange scientists to attend knowledge. Therefore, researchers in the group will conferences be supported to attend international/regional/local conferences where they will present papers Analytical and (oral/poster). One conference/workshop will be environmental chemistry organized. conference/ workshop to This items will also include priority support to female be arranged scientists for enabling them to participate in conferences.

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Exchange Visits by This activity is very important as it allows 27,000 27,000 25,000 Cooperating Scientists researchers’ close interactions/contacts for various Support to exchange purposes including visits for collaborative work, visits by scientists from planning, reporting, lecturing etc. This will cover at among the group members to another least one researcher’s visit per annum. university/laboratory/cou The support for exchange visits of cooperating ntry Scientists can be in both directions under South-South and North-South cooperation. Costs to be covered will include subsistence allowance and travels. Fellowships for Fellowships for training/exchange will involve short- 0 108,000 108,000 training/exchange term research visit of PhD students to Universities in Sweden or other country in Europe where they will conduct parts of their research using advanced techniques and get exposure to international research experience. This can also be used in cases of lack of/defective equipment in the local laboratory. Support to students The costs for sample analysis using sophisticated 30,000 30,000 30,000 Partial support to equipment are always very high and students in students for instrumental developing countries normally face challenges analysis costs during analyses. Partial support will be provided for instrumental analysis (e.g. GC-MS analysis) to meet the costs at a local laboratory or another laboratory in the region. Priority will be given to female students and self-sponsored students. This activity can enable atleast one student to spend up to one month in another country’s laboratory that is better equipped or in cases of faulty instruments/malfunctioning of equipment. Activities for There is no special budget line for activities for 0 0 0 improving gender improving gender balance. However, it should be balance emphasized that issues related to this item will be given priority in other budget lines (, e.g. through support to female scientists for participating in conferences, workshops, seminars, support to students for conducting research/analysis, applications for fellowships, etc. Audit costs and This item will cover audit fees and related expenses 10,000 10,000 25,000 Reference Group during external auditing of accounts and transport meeting costs for participating in the reference group meeting

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4. Staff and students in the group/network

List staff and students planned to be actively involved in the proposed activity.

a) List staff in the group/network Staff members who also are active students are to be listed ONLY under students.

Gender Given name, family name Position held Staff qualifications F/M / Function1 PhD Other Ac. Supp. M Dr. John A.M. Mahugija Research Group Leader X M Dr. Kessy F. Kilulya Deputy Leader X F Prof. Esther H. J. Lugwisha Researcher X F Dr. Monica M. Ndoile Researcher X F Dr. Regina Peter Researcher X F Dr. Grace A. Kinunda Researcher X M Dr. Faustin N. Ngassapa Researcher X M Dr. Fortunatus T. Sung'hwa Laboratory Scientist X M Lutufyo Mwamtobe Laboratory Scientist MSc M Michael Mayuni Laboratory Scientist MSc M Kassim Naswibu Researcher MSc F Twilumba Mlawa Laboratory technician X M Dr. O. Ilomo Laboratory Scientist X M Dr. Fredrick Lyatuu Researcher X (Insert more rows as needed, or just write below the table)

b) List students in the group/network (If already known) Students to be included should be those that benefit directly of the ISP support (fellowships) or indirectly by using consumables and equipment provided through the ISP support.

1 2 3 4 5 6 7 8a 8b F/M Name Target Start Thesis/Subject Grad Staff L/S D/I degree year year (Y/N) F Theresia John PhD 2017 Environmental 2021 N L I Lyasenga science/Environmental chemistry F Flaviana Ngabala MSc 2017 Chemistry 2019 Y L I

M Tumainieli Mwende MSc 2017 Chemistry 2019 N L I F Anastazia Nyakwesi MSc 2017 Biochemistry 2019 N L I Wandwi M Abrahaman Abrahman MSc 2017 Integrated environmental 2019 N L I Rashid management

M Faustine Gololi MSc 2018 Biochemistry 2020 N L I

1 Indicate deputy leader/coordinator, if applicable

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5. Scientific contacts/cooperation

Indicate which contacts with scientists working in the field of the proposed activities are established or planned (at your own university/institute, nationally, regionally, or internationally). The present and expected benefits of these contacts should be further described in the Research description (Enclosure 1).

a) List already established scientific contacts/collaboration that were engaged in active collaboration with your research group/network in 2016-2018.

Gender Title Name Affiliation Country F/M F Prof. Mayumi Ishizuka Hokkaido University Japan M Dr. M. M. Shouta Hokkaido University Japan M Dr. Osei Akoto Kwame Nkrumah University of Science and Technology Ghana M Dr. Godfred Darko Kwame Nkrumah University of Science and Technology Ghana M Dr. John Yabe University of Zambia Zambia M Prof. Yoshinori Ikenaka Hokkaido University Japan M Prof. Paul Fewou Moundipa University of Buea Cameroon M Prof. Lawrence I. Ezemonye University of Benin Nigeria F Dr. Isioma Tongo University of Benin Nigeria M Dr. Patrick Ssebugere Makerere University Uganda M Prof. Wageh Darwish Zagazig University Egypt F Dr. Edwige Kampire University of Rwanda-College of Education Rwanda F Dr. Doborah A. Abong’o University of Nairobi Kenya M Dr. John Wasswa Makerere University Uganda

a) List scientific contacts for collaboration you intend to establish with your research group/network in 2019-2021. TO BE DETERMINED; NOT CURRENTLY ESTABLISHED.

Gender Title Name Affiliation Country F/M F/M PROF, DR, MR, MISS RESEARCHERS UNIVERSITY SWEDEN F/M PROF, DR, MR, MISS RESEARCHERS UNIVERSITY GERMANY

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6. Other funding received (besides from ISP) 2016-2018, and available and foreseen funding for 2019-2021

Specify other funding obtained in 2016-2018, and other foreseen sources of funding for 2019-2021, from national as well as from other sources. List each source on a separate row, and give the amount for each year on separate rows. Estimate the amount in USD (currency conversion can be done at www.xe.com).

a) Other funding (besides from ISP) obtained in 2016-2018 Source Grant period/ Total Grant, mmyy-mmyy USD NIL

b) Other available or foreseen funding for 2019-2021 Source Grant period/ Total Grant, mmyy-mmyy USD UNIVERSITY OF DAR ES SALAAM JAN 2019- 4,000 JUNE 2019

7. Main equipment available

a) List relevant facilities and infrastructure available to the group. For equipment, give type and name of the manufacture of main items and other articles. Start with your most important equipment. Please, note if the equipment is not functioning properly and why.

Equipment type, model, and name of manufacturer Year Fully functional, yes/no acquired If no, indicate why GC-MS , QP 2010 Ultra, Shimadzu 2013 Yes AAS, Thermo Scientific AAS Model iCE 3000 2012 Yes FTIR Spectrometer, Platinum ATR, Alpha Brucker Yes Thermogravimetric analyser (TGA)/ Simultaneous Thermal 2012 Yes Analyser (STA)-FTIR, Linseis STA PT-1000 NMR Yes Surface Area & Pore Size Analyzer Yes HPLC, Konic HPLC 600, ROBOKROM LC 2012 No; it is defective Direct Mercury Analyzer, DMA-80 2017 Yes UV/Vis Spectrophotometers Yes Liquid Nitrogen Generator, MMR Elan2 Digital LN2 Liquefier 2017 Yes (Insert more rows as needed, or just write below the table)

b) List below main equipment needed in the near future, with justification. • Liquid Chromatography-Mass Spectrometer (LC-MS) • Rotary evaporator and accessories-for concentration of extracts • Gel Permeation Chromatograph (GPC)-for clean-up of complex sample matrices e.g. biota samples • Ultra low temperature laboratory freezer

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8. Environmental impact

Information is required about measures taken to reduce environmental impacts following the Environmental Impact Assessment for ISP as submitted to Sida in August 2009 (available on request).

a) Indicate which of the following measures your group/network has already implemented to reduce negative environmental impact.

Does your group/organization: Yes No A strategy to reduce negative environmental impact caused by travelling and X transportation? The use e-meeting techniques? X A strategy to reduce the use of electric power? X Considering environment impact criteria in procurement? X Practicing sorting of waste categories for recycling? X A system for scrapping decommissioned equipment? X A management system for chemical and hazardous waste? X Internal discussion of how any negative environmental impact of your activities can X be reduced? Engagement in external activities – in research, dissemination and/or society X outreach – on how negative environmental impacts may be reduced?

Comment on the no-answers in the previous question, if any. What are the reasons why some measures have not been implemented? Are there plans to do it in the future? Are there structural obstacles, such as regulations or lack of regulation/technology, etc.? Also comment on the practicing of measures in general to reduce negative environmental impact.

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ENCLOSURE 1: RESEARCH PLAN/NETWORK PROGRAM ANALYTICAL AND ENVIRONMENTAL CHEMISTRY RESEARCH GROUP CHEMISTRY DEPARTMENT, UNIVERSITY OF DAR ES SALAAM-TANZANIA

(a) Overall hypotheses/research questions, objective (impact) and relevance of the activity Analytical chemistry and environmental chemistry are specialities that have many application in many fields including chemistry, biochemistry, chemical engineering, environmental health, environmental analysis, forensic science, toxicology, industrial hygiene, medicine, pharmacology, pharmacy, geology, agriculture, and manufacturing industries (e.g. industrial quality control). There is an urgent need for training of highly qualified experts in analytical and environmental chemistry to meet the demands in increasing and growing institutions, industries, areas, activities and challenges that require applications of such knowledge and skills to solve the relevant problems. The general objective of the Analytical and Environmental Chemistry Research Group of the Chemistry Department, University of Dar es Salaam is strengthen the capacity for conducting scientific research and postgraduate training in various areas of analytical chemistry and environmental chemistry and apply the findings in solving problems in the societies/communities for development purposes. This activity is in line with the Tanzania’s Vision 2025, the National Research and Development Policy (2010) and the University of Dar es Salaam research policy (2015), and the Swedish Strategy for research cooperation and research in development cooperation 2015–2021.

(b) Specific hypotheses/research questions and objective The following are the specific hypotheses/research questions: (i) How to improve the conditions and techniques for carrying out research and postgraduate training in chemical analyses? (ii) What are the levels of chemical substances (e.g. contaminants) food, industrial materials and environmental samples (water, soil, sediments, biota and air)? (iii) How to convert bio-waste into useful products that can be used in various applications such as synthesis of alcohol and adsorbents? (iv) How to improve production and dissemination of high quality research results? (v) How to promote the use of research results and outcomes by the society (communities, industries, etc) and for development? (vi) How to attract sustainable financial support from various sources to ensure long-term stability? The research plans to answer these hypotheses/research questions through improved research training, experimentation, financial support, knowledge exchange and dissemination and use of results.

(c) Give specific objectives that should be reached within the period of support applied for The Analytical and Environmental Chemistry Research Group has the following specific objectives: (i) To improve the conditions and techniques for carrying out research and postgraduate training in chemical analyses. (ii) To carry out chemical analyses of food, industrial materials and environmental samples (water, soil, sediments, biota and air).

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(iii) To develop capacity in converting bio-waste into useful products that can be used in various applications such as synthesis of alcohol, natural gas purification, development of catalyst and adsorbents. (iv) To improve production and dissemination of high quality research results. (v) To promote the use of research results and outcomes by the society (communities, industries, etc) and for development. (vi) To attract sustainable financial support from various sources to ensure long-term stability.

In order to reach the goals set, the investigations in chemical analyses will involve MSc and PhD students and training programmes. The studies will include characterization of levels and chemistry of the chemical contaminants in food substances, industrial materials and environmental matrices such as surface water potable water, sediments, soil, air and biological samples (e.g., edible aquatic and terrestrial flora and fauna). Approval from an ethical committee will be obtained for studies that may raise ethical concern (e.g., those involving human subjects).

The methods for chemical analyses will include planning, sampling, sample preparation/processing, identification and quantification of the organic and inorganic analytes (e.g., pesticides, polycyclic aromatic hydrocarbons, alcohols, aldehydes, persistent organic pollutants, heavy metals, proteins, pharmaceuticals, aflatoxins, etc) in samples. Appropriate analytical methods will be developed and used. The development and application of appropriate analytical methods and techniques will be a key factor in the investigations. Emphasis will be placed on analytical quality assurance and control procedures to ensure reliability of the results. The developed methods will be validated using appropriate parameters. Standard methods will also be employed in the studies. The analytical quality assurance and control procedures will include use of high quality and analytical grade chemicals and standards, blank tests, recovery determinations, and determination of detection limits, limits of quantification, precision, accuracy, linearity and range of the methods, and other applicable procedures (European Commission, 2015). Qualitative and quantitative determination of the analytes will be carried out using appropriate techniques depending on the nature of the analytes, samples and other technical factors (Lugwisha et al., 2016; Mahugija et al., 2017; Mahugija and Njale, 2018; Mahugija and Sheikh, 2018). Generally, the analytical techniques will include gas chromatography mass spectrometry (GC-MS), liquid chromatography mass spectrometry (LC-MS), high performance liquid chromatography (HPLC), atomic absorption spectrometry (AAS), etc. Data analysis will be performed using different statistical methods depending on the nature of the data.

Various approaches will used to ensure wide dissemination of the research results including publications in high impact factor international and regional journals, conferences, workshops, symposia, seminars, meetings, media etc. To promote the use of research results by the government and society, meetings and discussions will be arranged as well as popular media and forums will be employed. In addition to the financial support expected from the ISP, funds will also be sought from other sources to ensure long-term stability of the research group activities. The research activities will be supported through various ways such as support for fellowships, exchange visits, instrumental analysis costs, provision of consumables (e.g. solvents, reagents and reference standards), field works and participating in conferences and outreach activities.

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(d) Planned outputs and expected outcomes The planned outputs will include training/supporting of at least 1 PhD and 6 MSc students, producing at least 7 publications in international journals, conducting 5 contributions to conferences, organizing 1 conference, purchasing sample preparation equipment, purchasing consumables (standards, solvents, reagents, etc) and conducting 3 scientific exchanges. The expected outcomes will include improved and increased research activities and training, availability of minor equipment, increased number of experts in analytical and environmental chemistry, increased graduations, high quality research results, and improved dissemination and use of use of research results by the society.

(e) Postgraduate education plan The planned postgraduate education activities will involve partial support to meet import costs for consumables, literature, field works, instrumental analysis and participating in conferences. One PhD fellowship is also planned for short-term research scholarship to a relevant institution/laboratory in Sweden or other country in Europe (up to 3 months).

(f) Gender balance The current gender balance in the research group is generally reasonable as the females represent 40% of the members and for the students the ratio is 50:50. To continue improving the gender balance, special priority and consideration on the support opportunities under the proposed research activities will be given to female students.

(g) Other expected funding Other expected sources of funding are the University of Dar es Salaam, employers of the students and private sources (from parents and relatives). Funding will also be applied from other donors including DAA and OPCW. The University of Dar es Salaam is expected to contribute with infrastructure (office, utilities etc) and staff salaries.

(h) Collaboration with other scientists The proposed activity will benefit from the contacts and collaboration already established with other scientists or which will be established through various national and international institutions such as academic networks (e.g., SETAC, ANCAP, TCS, E-SALAMA, SEANAC, WIOMSA), Universities (Addis Ababa University, Universities of Nairobi, Makerere University, University of Johannesburg, University of KwaZulu Natal, University of Zambia, University of Zimbabwe, Hokkaido University, Kwame Nkrumah University of Science and Technology, Zagazig University, University of Benin, University of Buea, Cameroon, etc.).

(i) Postgraduate students 1. Anastazia Nyakwesi Wandwi (F), MSc (Biochemistry) Proposed dissertation title: Identification and quantification of drugs and metabolites of drugs of abuse from urine samples by LC-MS-MS; started July 2017; the expected date for presenting the thesis is 2019. She is conducting laboratory work at the Government Chemist Laboratory, but recently the LC-MS-MS they were using malfunctioned and so she is waiting for it to be fixed. Support may be needed. 2. Flaviana Ngabala (F), MSc (Chemistry) Proposed dissertation title: Effects of industrial processing on the levels of pesticide residues in tomatoes and tea, July 2017 to date; the expected date for presenting the thesis is 2019. Her research proposal was approved, but she has been unable to proceed with field work for more than 5 months because of lack of funds from her local sponsor. She needs financial assistance to proceed with her research. Page 3 of 5

3. Tumainieli Mwende (M), MSc (Chemistry) Proposed dissertation title: Optimization of conditions for bioethanol extraction from agricultural wastes in Dar es Salaam market, started July 2017; the expected date for presenting the thesis is 2019. He started field work but is facing problems. He needs partial support to carry out the research successfully. 4. Theresia J. Lyasenga (F), PhD (Environmental Sciences) Proposed dissertation title: Assessment of levels of compounds resulting from petroleum wastes in Lake Victoria basin and their effects on food security; started July 2017; the expected date for presenting the thesis is 2021.This student is currently developing a research proposal but is facing serious lack of funds for supporting her dissertation phase. She is self- sponsored and seeking support to continue with her studies. She is expecting to apply for fellowship from ISP and is one of the students planned to be partially supported if this proposal will be granted; otherwise this student will probably fail to continue with the study. Therefore, ISP is requested to consider supporting her through the applied project. 5. Abrahaman A. Rashid (M), (Master of Integrated Environmental Management) Proposed dissertation title: Assessment of physico-chemical and microbiological parameters of groundwater in rural and urban areas of Wete District, Pemba, started July 2017; the expected date for presenting the thesis is 2019. He started field work but is facing problems obtaining funds for laboratory analyses of samples as he is self- sponsored. Can be partially supported through the applied project. 6. Faustine Gololi (M), MSc (Biochemistry): Currently developing a research proposal.

(j) Justification of budget items The budget items have been justified in Section 3d of the filled Grant Application Form.

(k) Background The Analytical and Environmental Chemistry Research Group at the Chemistry Department, UDSM was formed about five years ago after merging the analytical chemistry and environmental chemistry groups that previously existed in order to consolidate the efforts and improve research capacity as well as address the numerous challenges to be that were faced by the two groups such as low level of research funding, inadequate human resource base and inadequate infrastructure. The environmental chemistry group was formerly supported by the ISP (TAN: 02) in 2004 to 2008. This year, the Director, ISP invited the Chemistry Department to submit an application for consideration for the 2019-2021 grants. Hence, this grant application is submitted in response to that call. The research group at the Chemistry Department is composed of competent and capable staff with numerous specializations within the areas of analytical chemistry, environmental chemistry and related areas in chemistry that are ready to undertake the research activities. The qualified students to be engaged in the proposed activity are available. There is great need for further training as there are currently many institutions in the country that need chemists including the research institutions, universities, industries, Tanzania Food and Drugs Authority, Government Chemist Authority, Energy sector, mining industry, etc. The department has most of the equipment needed for conducting the activities. However, some equipment are defective or are missing and therefore some minor equipment and other facilities will be required to be purchased in order to successfully carry out the proposed activity. The specification of the minor equipment and other facilities to be purchased are detailed in the budget and its justification.

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References European Commission, EU, (2015) Guidance Document on Analytical Quality Control and Validation Procedures for Pesticide Residues Analysis in Food and Feed, Document No. SANTE/11945/2015 Supersedes SANCO/12571/2013 of 2013, EU. Lugwisha, E.H.J., Mahugija, J.A.M. and Mwankuna, C., 2016. Levels of formaldehyde and acetaldehyde in selected bottled drinking water sold in urban areas in Tanzania. Tanzania Journal of Science 42, 1–14. Mahugija, J.A.M., Kayombo, A. and Peter, R., 2017. Pesticide residues in raw and processed maize grains and flour from selected areas in Dar es Salaam and Ruvuma, Tanzania. Chemosphere 185, 137–144. Mahugija, J.A.M. and Njale, E., 2018. Levels of polycyclic aromatic hydrocarbons (PAHs) in smoked and sun-dried fish samples from areas in Lake Victoria in Mwanza, Tanzania. Journal of Food Composition and Analysis 73, 39-46. Mahugija, J.A.M. and Sheikh, H.M., 2018. Status of selected heavy metals dispersion from top soil in and around automobile workshop areas in Zanzibar Municipality, Tanzania. Tanzania Journal of Science 44 (1), 12-23. University of Dar es Salaam (2015) UDSM Research Policy and Operational Procedures.

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CURRICULUM VITAE

1. Personal Particulars Name: Dr. John Andrew Marco Mahugija Sex: Male Date and Place of Birth: 16th July 1971, Kwimba District, Mwanza–Tanzania Nationality: Tanzanian Marital Status: Married Languages: English and Swahili Contact/Address: Chemistry Department, University of Dar es Salaam P.O. Box 35061, Dar es Salaam – Tanzania Mobile Phones: +255 719 971 971 & +255 766 971 971 E-Mail: [email protected]; [email protected]

2. Education 2007 – 2011 Ph.D. (Chemistry); Specialization: Analytical and Environmental Chemistry, University of Dar es Salaam/Institute of Ecological Chemistry-German Research Centre for Environmental Health, Munich. 2001 – 2004: M.Sc. (Chemistry); Specialization: Analytical and Environmental Chemistry, University of Dar es Salaam/Institute of Ecological Chemistry, GSF- National Research Centre for Environment and Health, Munich-Germany. 1997 – 2001: B.Sc. (Ed) (First Class Honours), Major subjects: Biology, Chemistry, and Education; University of Dar es Salaam. 1991 – 1993: Advanced Certificate of Secondary Education in Chemistry, Biology, Geography, Siasa [Politics] and Basic Applied Mathematics, Tosamaganga High School, Iringa–Tanzania. 1987 – 1990: Certificate of Secondary Education; Sengerema Secondary School, Mwanza. 1980 – 1986: Certificate of Primary Education; Chanongu Primary School, Kwimba District, Mwanza–Tanzania.

3. Academic Prizes/Awards and Distinctions 3.1 Academic Prizes/Awards 1. German Academic Exchange Service (DAAD)/ University of Dar es Salaam Scholarships to conduct Ph.D. (Chemistry) studies at the University of Dar es Salaam and at the Institute of Ecological Chemistry-German Research Centre for Environmental Health, Munich in 2006/07–2009/2010. 2. German Academic Exchange Service (DAAD) Scholarships to conduct M.Sc. (Chemistry) studies at the University of Dar es Salaam and at the Institute of Ecological Chemistry, GSF- National Research Centre for Environment and Health, Munich-Germany in 2001–2004. 3. University Prize–for being the best final year student in the Faculty of Science, University of Dar es Salaam in 2001. 4. Thermodynamics Prize–for being the best second year student in Thermodynamics, University of Dar es Salaam. 5. Chemistry Prize–for being the best second year student in Chemistry in 1998/1999, University of Dar es Salaam. 6. Tanzania Breweries Limited (TBL) Prize – Best final year student in the Faculty of Science, University of Dar es Salaam in 2000/2001. 7. Double Cola Cash Prize – Best final year student in the Faculty of Science, University of Dar es Salaam 2001. 8. Ministry of Science, Technology and Higher Education Prize - for being the overall best final year student in the Faculty of Science, University of Dar es Salaam 1997–2001. 1 9. Coca Cola Kwanza Prize - for being the overall best final year student in B.Sc.(Ed). 10. Tosamaganga High School Award–for being the best student in Biology in 1991–1993. 11. Sengerema Secondary School Award–for being the best student in form four examinations in 1990. 12. Chanongu Primary School Award–for being an overall best pupil in academic performance in all subjects from 1980 to 1986. 3.2 Distinctions 1997/1998: Best student in Education in first year in undergraduate studies. 1998/1999: Best second year student in Chemistry, best second year student in Biology and best second year student in B.Sc.(Ed). 1999/2000: Best third year student in B.Sc.(Ed). 2000/2001: Best student in fourth year Chemistry, best student in fourth year Biology, best student in Education in fourth year, best fourth year student in B.Sc.(Ed), overall best final year student in Education, overall best final year student in B.Sc.(Ed), and overall best final year student in the Faculty of Science in 1997–2001, University of Dar es Salaam.

4. Posts held at the University of Dar es Salaam  Senior Lecturer in Chemistry, 1st July 2015 to date.  Lecturer in Chemistry, 1st July 2009 to 30th June 2015.  Assistant Lecturer in Chemistry, 1st July 2005 to 30th June 2009.  Representative member in the University Students Representative Council (USRC) – University of Dar es Salaam, September 1998–August 2001.  In-charge of Pesticides and Persistent Organic Pollutants (POPs) Analysis Laboratory, Chemistry Department, October 2005 to date.  Member, Examination Moderation Committee, Chemistry Department 2010 to date.  Coordinator of the PhD Programme in Environmental Sciences, College of Natural and Applied Sciences, July 2015 to date.  Coordinator and Member of the Steering Committee of the Master of Science in Integrated Environmental Management (M.Sc. IEM) programme, formerly known as Master of Integrated Environmental Management (MIEM), College of Natural and Applied Sciences, July 2015 to date.  Member, Higher Degrees Research and Publication Committee, Chemistry Department 2010–2013, 2016 to date.  Member, Curriculum Review Committee, Chemistry Department 2010–2013.  Timetable Coordinator, Chemistry Department 2009–2013.  Recorder Departmental Meetings, Chemistry Department 2010–2013.  Internal Examiner of M.Sc. Theses and Dissertations 2013 to date.  Independent Internal Examiner for the Faculty of Science of the Mkwawa University College of Education (MUCE) for the 2016/2017–2017/2018 academic years.

5. Posts held Outside the University of Dar es Salaam  Teacher, Biology and Chemistry, Nyamilama Secondary School, Mwanza–Tanzania, October 1994 –September 1997.  Teacher, A-Level Chemistry, Mzumbe High School, Morogoro–Tanzania, February 2002– April 2003.  Teacher, A-Level Chemistry, Pugu High School, Dar es Salaam, May 2003 to June 2005.  Part-time Lecturer, Mkwawa University College of Education, Iringa: General Chemistry (CH 010) 2006–2008, Basic Analytical and Physical Chemistry (CH 118) 2011–2013 and Analytical and Environmental Chemistry (CH 262) 2012–2013.

2  Part-time Lecturer, Dar es Salaam University College of Education: Analytical and Environmental Chemistry (CH 262) 2010–2011 and Environmental Science–Environmental pollution, degradation and toxicology (EV 200) 2010–2012.  Part-time Lecturer, ; Basic Chemistry (ES 112) 2012 – 2014.  Part-time Lecturer, St. Joseph College of Engineering-Dar es Salaam: Principles of Environmental Science and Engineering (99GE106), 2010/2011.  Part-time Lecturer, University of Bagamoyo; Fuel Chemistry and Technology, 2014–2015.  External Examiner of The Open University of Tanzania (MSc Thesis 2014), Sokoine University of Agriculture (PhD Thesis 2018).  External Examiner & Special duties of Chemistry for the National Examinations Council of Tanzania (NECTA), 2015 to date.

6. Administrative Posts held Outside the University of Dar es Salaam  Treasurer and Assistant Executive Secretary, African Network for the Chemical Analysis of Pesticides (ANCAP), January 2012 to date.  President, Sengerema Secondary School Alumni Society (SESESCO Alumni Society), 8th May 2016 to date. Also founder member from 16th January 2016.  Chairperson, School committee, St. Don Bosco School, Sinza-Dar es Salaam, 7th May 2016 to date.  Chairperson, School committee, Shekilango Primary School, Kinondoni-Dar es Salaam, 26th May 2012 to 16th July 2016.  Education Officer – Environmental Education, Ministry of Education and Culture Head Quarters, Dar es Salaam, 27th December 2004 – 30th March 2005. 7. Professional Affiliation 1. Founder Member, African Network for the Chemical Analysis of Pesticides (ANCAP) (July 2002 to date) & Treasurer and Assistant Executive Secretary of ANCAP 2012 to date. 2. Member, Western Indian Ocean Marine Science Association (WIOMSA), 7th December 2005 to date. 3. Member, Society of Environmental Toxicology and Chemistry (SETAC), October 2006 to date. 4. Member, World Academy of Young Scientists (WAYS), February 2007 to 2017. 5. Assistant Executive Secretary and Treasurer, African Network for the Chemical Analysis of Pesticides (ANCAP), January 2012 to date. 6. Reviewer of Journals (2014 to date): e.g., Tanzania Journal of Science, Journal of Environmental Monitoring and Assessment (Published by Springer), Water SA, South Africa, Journal of Food Composition and Analysis (Published by Elsevier) and African Journal of Microbiology Research (Published by Academic Journals). 8. Teaching/ Supervision 8.1 Teaching/Supervision Undergraduate Courses Basic Analytical and Physical Chemistry (2010–); Analytical and Environmental Chemistry (2010–); Project Work (2010– ); The Chemistry of Coal (2014– ); Chemistry Practicals (1st to 3rd year practical courses) (2010– ); Petroleum Chemistry Practicals (2014– ); Chemical Waste Management (2011–2017); Fuel Chemistry and Technology (2011–2012); Environmental Analytical Chemistry (2006–2010); and Environmental Science (2005–2008).

8.2 Teaching of Postgraduate Courses  EV 718: Methods in Environmental Chemical Analysis (Course for the PhD programme in Environmental Sciences), 2014–. 3  CH 630: Advanced Analytical Chemistry and Instrumentation (Course for M.Sc. Chemistry), 2011–2012.  IEM 630: Environmental Chemistry (Course for Master of Integrated Environmental Management), 2006–2007.

8.3 Supervision of Postgraduate Students 1. Lutamyo Nambela (F), M.Sc. (Chemistry) Thesis: Levels and chemodynamics of pesticide residues in Eastern Lake Tanganyika Basin, Tanzania, July 2011 to Sept 2014. 2. Christopher Mwankuna (M), M.Sc. (Chemistry) Dissertation: Determination of formaldehyde and acetaldehyde in selected bottled drinking water sold in urban areas in Tanzania, July 2011 to October 2014. 3. Hawa M. Sheikh (F), M.Sc. (Chemistry) Dissertation: Assessment of the status of heavy metal dispersion from top soil in and around automobile workshop areas in Zanzibar municipality, Tanzania, July 2012 to October 2014. 4. Auguster Kayombo (F), M.Sc. (Education) Dissertation: Assessment of levels of pesticides residues in raw and processed maize grains and flour from selected areas in Dar es Salaam and Ruvuma, July 2013 to October 2014. 5. Farhat A. Khamis (F), M.Sc. (Chemistry) Dissertation: Assessment of levels of pesticides residues in fruits and vegetables from markets in Dar es Salaam, July 2013 to November 2014. 6. Kamaria N. Ahmed (F), M.Sc. (Chemistry) Dissertation: An investigation on Polycyclic Aromatic Hydrocarbons (PAHs) contamination in mangrove ecosystems of Zanzibar coastal areas, July 2012 to October 2015. 7. Patrick E. Chibura (M), M.Sc. (Chemistry) Dissertation: Assessment of pesticides residues in poultry products and feed in selected poultry farms in Ilala and Kibaha Districts, Tanzania, July 2014 to July 2016. 8. Zainab S. Kasenya (F), M.Sc. (Chemistry) Dissertation: Determination of levels of heavy metals in urine among primary school pupils living in selected industrial and non-industrial areas in Dar es Salaam, July 2015 to October 2017. 9. Emmanuel Njale (M), MSc. (Chemistry) Dissertation: Levels of Polycyclic Aromatic Hydrocarbons (PAHs) in smoked and unsmoked fish from areas around Lake Victoria in Mwanza and the effects of washing, July 2016 to May 2018. 10. Anastazia Nyakwesi Wandwi (F), MSc (Biochemistry) Dissertation: Identification and quantification of drugs and metabolites of drugs of abuse from urine samples by LC- MS/MS, July 2017 to date. 11. Flaviana Ngabala (F), M.Sc. (Chemistry) Dissertation: Effects of industrial processing on the levels of pesticide residues in tomatoes and tea, July 2017 to date. 12. Tumainieli Mwende (M), M.Sc. (Chemistry) Dissertation: Optimization of conditions for bioethanol extraction from agricultural wastes in Dar es Salaam market, July 2017 to date. 13. Theresia J. Lyasenga (F), PhD (Environmental Sciences) Dissertation: Assessment of levels of compounds resulting from petroleum wastes in Lake Victoria basin and their effects on food security, July 2017 to date. 14. Abrahaman A. Rashid (M), Master of Integrated Environmental Management Dissertation: Assessment of physico-chemical and microbiological parameters of groundwater in rural and urban areas of Wete District, Pemba, July 2017 to date.

9. Research and Consultancy Fields 9.1 Current Research/Consultancy Fields/Research Interests

4  Analytical and environmental chemistry: Analysis of organic and inorganic chemicals such as pesticides, Polychlorinated Biphenyls (PCBs), Polycyclic Aromatic Hydrocarbons (PAHs), aldehydes and heavy metals in samples of environmental matrices (i.e., water, soil, sediments, biota and air), food substances, industrial products and others.  Development and application of analytical methods for organic and inorganic compounds and study the environmental chemodynamics and effects of xenobiotic compounds.  Development, characterization, and application of methods for remediation of chemical pollutants.

9.2 Major Projects 1. The Food Security Programme 2009–2014: Principal investigator, Analysis of pesticides and persistent organic pollutants in the environment and edible biota in Tanzania, funded by SIDA. 2. The African Network for the Chemical Analysis of Pesticides (ANCAP) programme 2002 to date; funded by the International Science Programme (ISP), Sweden: Principal investigator and administrator.

10. Conferences, Workshops, Training Courses and Participation in Public Activities 10.1 Conferences, Workshops, Training and Courses 1) February–June 1988: Participated in militia training course, Sengerema-Mwanza, Tanzania. 2) July–December 1993: National service training (Jeshi la Kujenga Taifa) at Oljoro (Arusha) and Maramba (Tanga) Camps, Tanzania. 3) May–June 2001: Studied a computer course in the Faculty of Science, University of Dar es Salaam. Competent with most computer programmes such as Word, Excel, Access, Powerpoint, Internet, Scanning, ChemDraw, etc. 4) January 27th–2nd February 2002: Participated in a workshop on planning for research activities on pesticide residues and other environmental pollutants, held in Uppsala and Stockholm, Sweden. 5) 23rd–26th September 2002: Participated in an international scientific symposium on environment, Faculty of Science, University of Dar es Salaam – Tanzania. 6) 1st October–30th November 2003: German Academic Exchange Service (DAAD) Scholarship. Conducted a research involving GC-MS and LC-MS-MS analyses of pesticide residues and other organic micropollutants in surface waters, food crops, animal feeds, plant parts, soil and sediments samples at the Institute of Ecological Chemistry, GSF-National Research Centre for Environment and Health, Munich - Germany. 7) 28th–29th May 2004: Participated in the German Academic Exchange Service (DAAD) Alumni Seminar on adapted technologies for environmental protection and international cooperation, White Sands Hotel, Dar es Salaam, Tanzania. 8) 2nd–7th August 2004: Participated in ANCAP summer school on pesticide chemistry, toxicology and instrumental analytical methods at the Tropical Pesticide Research Institute (TPRI), Arusha, Tanzania. 9) 8th–11th August 2004: Participated in the African Network for the Chemical Analysis of Pesticides (ANCAP) Inaugural Conference, AICC – Arusha, Tanzania. 10) November 2004 – March 2005: Organized and participated in a workshop on promotion of environmental education for sustainable development in Tanzania, held on 15th–17th March, 2005 at Mkonge Hotel, Tanga – Tanzania. 11) 21st January 2005: Participated in a regional workshop on nitrogen, phosphorus and sulphur derivatives in the environment, Hotel Africana, Kampala – Uganda. 12) 11th–20th July 2005: Participated in ANCAP summer school on pesticide chemistry and residue analysis at Makerere University, Kampala-Uganda. 5 13) 21st November–2nd December 2005: Participated in a training course on aquatic ecotoxicology and risk assessment of agrochemical pollution in western Indian Ocean coastal marine ecosystems at the Institute of Marine Sciences, Zanzibar. 14) 12th–15th October 2006: Participated in the 5th ANCAP summer school on cyberchemistry, pesticide registration, and the use of multivariate techniques for analyzing ecological and ecotoxicological data, TPRI-Arusha, Tanzania. 15) 16th–20th October 2006– Participated in an international conference on pesticide use in developing countries: Environmental fate, effects and public health implications, Arusha International Conference Centre–Tanzania. 16) 21st–22nd October 2006: Participated in a workshop on the development of scientific projects organized by The International Foundation for Science (IFS) held at TPRI-Arusha, Tanzania. 17) 21st–22nd March 2007: Participated in launching and assembly of the World Academy of Young Scientists (WAYS–Africa), ABSA Conference Centre in Pretoria, South Africa. 18) 11–12th August 2008: Participated in the 20th International Conference on Chemical Education (ICCE) satellite workshop held at the Department of Chemistry, University of Nairobi, Kenya. 19) 24th–25th October 2008: Participated in a seminar on “Knowledge and Research for Development in East Africa” organized jointly by the German Academic Exchange Service (DAAD) and Alexander von Humboldt Foundation (AvH-), held at the Grand (Laico) Regency Hotel Nairobi, Kenya. 20) 8th–11th November 2008: Participated in the First African Congress on Pesticides and Toxicology Sciences, held at the University of Gezira, Wad Medani, Gezira State-Sudan. 21) 2nd May–31st October 2009: German Academic Exchange Service (DAAD) Scholarship. Conducted a research involving analysis of pesticides, PCBs, and PAHs in soil and water samples using advanced techniques such as Accelerated Solvent Extraction (ASE) and High Resolution GC-MS at the Institute of Ecological Chemistry, German Research Centre for Environmental Health (HelmholtzZentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt), Munich–Germany. 22) 8th–9th September 2010: Participated as a presenter and a commentator in the 2nd International Toxicology Symposium in Africa which was jointly organized by Schools of Veterinary Medicine of University of Zambia and Hokkaido University-Japan, held at the University of Zambia, Lusaka – Zambia. 23) 16th–27th May 2011: Participated in the analytical chemistry course on the principles, procedures, and applications of gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) for the analysis of chemicals related to the Chemical Weapons Convention. The course was organized and supported jointly by the Organization for the Prohibition of Chemical Weapons (OPCW), Protechnik Laboratories-South Africa, and the Finnish Institute for the Verification of the Chemical Weapons Convention (VERIFIN) and held at Protechnik laboratories in Pretoria, South Africa. 24) 10th–13th October 2011: Participated in the Regional Scientific Symposium on pesticides research strategies and plans, organized by The African Network for the Chemical Analysis of Pesticides (ANCAP), held at Mazsons Hotel, Zanzibar. 25) 2nd–4th September 2012: Participated as a presenter and a commentator in the 4th International Toxicology Symposium in Africa which was jointly organized by Schools of Veterinary Medicine of University of Zambia and Hokkaido University-Japan, held at the University of Zambia, Lusaka – Zambia. 26) Sept 2012–Jan 2013: Organized and participated in the ANCAP 10th Anniversary Regional Symposium held on 4th –7th January 2013 at the University of Dar es Salaam, Tanzania. 27) 19th–24th May 2013: Held discussions about research collaboration and visited research activities at the University of Dhaka in Bangladesh. 28) 2nd–3rd September 2013: Participated in the 6th SETAC Africa conference held at the Intercontinental Hotel in Lusaka, Zambia.

6 29) 11th–14th September 2013: Participated as a presenter and a commentator in the 5th International Toxicology Symposium in Africa held at the Kwame Nkrumah University of Science and Technology in Kumasi, Ghana. 30) August-December 2014: Organized the 7th Symposium of the African Network for the Chemical Analysis of Pesticides (ANCAP) held on 20th - 22nd December 2014, Reef Hotel Mombasa, Kenya. 31) 28th–30th August 2014: Participated as a presenter and a commentator in the 6th International Toxicology Symposium in Africa held at the Garden Court, O.R. Tambo International Airport in Johannesburg, South Africa. 32) June-July 2015: Organized, participated, facilitated and coordinated the 11th Summer School and 8th Symposium of the African Network for Chemical Analysis of Pesticides (ANCAP) held on 27th – 31st July 2015 at Makerere University, in Kampala, Uganda. 33) 30th–31st August 2015: Participated as a presenter and a commentator in the 7th International Toxicology Symposium in Africa held at the Garden Court, O.R. Tambo International Airport in Johannesburg, South Africa. 34) 6th October 2015: Presented a lecture on "qualities of effective teaching and bad teaching in higher education" at the Faculty Development Programme of St Joseph University in Tanzania held at St Joseph College of Engineering in Dar es Salaam. 35) 25th–26th February 2016: Participated in the International Union of Pure and Applied Chemistry (IUPAC) Ecological Risk Assessment of Pesticides Workshop in East Africa held at the Technical University of Kenya, Nairobi, Kenya. 36) 29th–31st August 2016: Participated as a presenter and a commentator in the 8th International Toxicology Symposium in Africa held at Gawharet Al Ahram hotel in Giza- Cairo, Egypt. 37) 3rd October 2016: Participated in the National stakeholders’ workshop for the endorsement of the revised National Implementation Plan for the implementation of the Stockholm Convention on Persistent Organic Pollutants (POPs), organized by the Vice-President’s Office Division of Environment held at Sokoine University Morogoro, Tanzania. 38) September-November 2016: Organized, participated, facilitated and coordinated the 9th Symposium of the African Network for Chemical Analysis of Pesticides (ANCAP) held on 21st – 24th November 2016 at University of Nairobi, Kenya. 39) 17th–27th April 2017: Participated in the capacity building training course on oil, gas and coal held at the University of Aberdeen Scotland, United Kingdom. 40) 12th April 2017 & 11th May 2017: Participated in the National Inception Workshop on Disposal of Polychlorinated Biphenyls (PCBs) Oils contained in Transformers and Disposal of Capacitors containing PCBs in Southern African Countries organized by the Vice-President’s Office Division of Environment held at TANESCO Training Centre in Dar es Salaam, Tanzania. 41) 07th–08th September 2017: Participated as a presenter and a commentator in the 9th International Toxicology Symposium in Africa held at the Constantial Hotel & Suites in Benin City, Nigeria. 42) September-November 2017: Organized the 10th Symposium of the African Network for Chemical Analysis of Pesticides (ANCAP) held on 2nd to 4th December 2017 at The Reef Hotel, Mombasa, Kenya. 43) 10th to 12th July 2018: Participated in the national workshop to raise awareness of the National Environmental Research Agenda (NERA 2017-2022) organized by the National Environment Management Council (NEMC) held at the National Carbon Monitoring Centre (NCMC), Sokoine University of Agriculture (SUA) Morogoro.

10.2 Participation in Public Activities and Special Duties (Selected Major Events) 1) 26th June 2010: Guest of honour at fund-raising party for building classrooms at Buchamwa Primary School in Kwimba District–Mwanza, Tanzania.

7 2) 12th August 2012: Guest of honour at fund-raising party at Kagera Catholic Church Usagara–Mwanza, Tanzania. 3) 27th October 2012: Guest of honour at form four graduation ceremony at GG Shulua Secondary School in Dar es Salaam, Tanzania. 4) 25th January 2015: Guest of honour at fund-raising party at Kagera Catholic Church Usagara–Mwanza, Tanzania. 5) 18th –23rd April; 5th July–8th August & 8th-11th September 2016: Participated in editing of manuscripts of chemistry text books for Advanced level (form five and six) at the Tanzania Institute of Education, Dar es Salaam. 6) 29th February–14th March 2016, 11th–20th October 2017 and 22nd August –6th September 2018: Performed special duties in the Chemistry Panel of the National Examinations Council of Tanzania (NECTA) in Dar es Salaam. 7) 25th to 30th January 2018: Chairperson of chemistry panel and evaluator of educational reference materials for secondary schools at the Tanzania Institute of Education (TIE), Dar es Salaam. 8) 10th to 20th February 2018: Participated in writing chemistry tutor’s manual for teachers’ colleges in Tanzania in the workshop organized by the Ministry of Education, Science and Technology and held at Morogoro Teachers’ College. 9) 27th to 29th March 2018: Chairperson of chemistry panel and evaluator of educational reference materials for secondary schools in the workshop organized by the Tanzania Institute of Education (TIE) and held at the Agency for the Development of Educational Management (ADEM)-Bagamoyo, Tanzania. 10) 26th February to 15th March 2018: Instructor in the training on environmental sampling and analysis for the National Environment Management Council (NEMC) Staffs held at the Chemistry Department, University of Dar es Salaam. 11) 29th March to 27th April 2018: Collection and analysis of water samples from Export Processing Zones Authority (EPZA) and Twiga Cement Industry (Wazo) in Dar es Salaam: Consultancy for the National Environment Management Council (NEMC). 12) 2015–2017: External Examiner of Chemistry for the National Examinations Council of Tanzania (NECTA) Certificate of Secondary Education Examinations (CSEE) 2015 (All papers), 2016 (Practical), 2017 (Practical) marking held in Dar es Salaam (4th–13th December 2015), Tabora (30th November–14th December 2016), Masasi-Mtwara (2nd–16th December 2017). 13) 2016–2018: External Examiner of Chemistry for the National Examinations Council of Tanzania (NECTA) Advanced Certificate of Secondary Education Examinations (ACSEE) 2016 (Practical), 2017 (All papers), 2018 (Practical) marking held in Morogoro (30th May– 8th June 2016), Dodoma (27th May–05th June 2017), Morogoro (28th May–06th June 2018). 14) 6th to 9th August 2018: Chairperson of chemistry panel and evaluator of educational reference materials for secondary schools in the workshop held at the Tanzania Institute of Education (TIE). 15) 16th to 20th August 2018: Chairperson and evaluator of science and technology text books for standard five in the workshop held at the Tanzania Institute of Education (TIE).

11. Publications 11.1 Publications in Refereed Journals 11.1.1 Mahugija-Marco, J.A. and Kishimba, M.A., 2005. Concentrations of pesticide residues in grasses and sedges due to point source contamination and the indications for public health risks, Vikuge, Tanzania. Chemosphere 61, 1293–1298, Elsevier. 11.1.2 Mahugija-Marco, J.A. and Kishimba, M.A., 2006. Pesticides and metabolites in cassava, eucalyptus, plum and cashew leaves and roots in relation to a point source in Kibaha, Tanzania. Chemosphere 64, 542–548, Elsevier.

8 11.1.3 Mahugija-Marco, J.A. and Kishimba, M.A., 2007. Organochlorine pesticides and metabolites in young leaves of Mangifera indica from sites near a point source in Coast Region, Tanzania. Chemosphere 68, 832–837, Elsevier. 11.1.4 Mahugija-Marco, J.A. and Kishimba, M.A. 2007. Distribution of pesticide residues in soil and sediments from areas of Coast Region near a heavily contaminated site in Tanzania. International Journal of BioChemiPhysics 16, 7–16. 11.1.5 Mahugija-Marco, J.A. and Kishimba, M.A. 2007. Contamination of surface waters by obsolete pesticides emanating from a point source in Coast Region, Tanzania. International Journal of BioChemiPhysics 16, 17–26. 11.1.6 Ssebugere, P., Wasswa, J., Mbabazi, J., Nyanzi, S.A., Kiremire, B.T. and Mahugija- Marco, J.A., 2010. Organochlorine pesticides in soils from south-western Uganda. Chemosphere, 78(10), 1250–1255, Elsevier. 11.1.7. Mahugija, J.A.M., 2012. Status of pesticides and degradation products in soil after clean-up of stockpiles and treatment with sodium hydroxide at a former storage site in Korogwe, Tanzania. Tanzania Journal of Science 38(3), 27–40. 11.1.8 Mahugija, J.A.M., 2012. Organochlorine pesticides and degradation products in soil around a former formulation plant in Morogoro municipality, Tanzania. Tanzania Journal of Science 38(3), 41–53. 11.1.9 Mahugija, J.A.M., 2013. Status and distributions of pesticides buried at five sites in Arusha and Mbeya regions, Tanzania. African Journal of Pure and Applied Chemistry 7 (12), 382–393, Academic Journals. 11.1.10 Mahugija, J.A.M., Henkelmann, B. and Schramm, K.W., 2014. Levels, compositions and distributions of organochlorine pesticide residues in soil 5 –14 years after clean-up of former storage sites in Tanzania. Chemosphere 117, 330–337, Elsevier. 11.1.11 Mahugija, J.A.M., Henkelmann, B. and Schramm, K.W., 2015. Levels and patterns of organochlorine pesticides and their degradation products in rainwater in Kibaha Coast Region, Tanzania. Chemosphere 118, 12–19, Elsevier. 11.1.12 Mahugija, J.A.M., 2015. Levels and profiles of polycyclic aromatic hydrocarbons and polychlorinated biphenyls in soil at an industrial area in Dar es Salaam, Tanzania. International Journal of Humanities, Arts, Medicine and Sciences 3(6), 1–7, Best Journals. 11.1.13 Lugwisha, E.H.J., Mahugija, J.A.M. and Mwankuna, C., 2016. Levels of formaldehyde and acetaldehyde in selected bottled drinking water sold in urban areas in Tanzania. Tanzania Journal of Science 42, 1–14. 11.1.14 Mahugija, J.A., Khamis, F.A. and Lugwisha, E.H., 2017. Determination of levels of organochlorine, organophosphorus, and pyrethroid pesticide residues in vegetables from markets in Dar es Salaam by GC-MS. International Journal of Analytical Chemistry vol. 2017, 9 pages, Hindawi Publishing Corporation. 11.1.15 Mahugija, J.A.M., Kayombo, A. and Peter, R., 2017. Pesticide residues in raw and processed maize grains and flour from selected areas in Dar es Salaam and Ruvuma, Tanzania. Chemosphere 185, 137–144. 11.1.16 Mahugija, J.A.M., Khamis, F.A. and Lugwisha, E.H.J., 2017. Assessment of pesticide residues in tomatoes and watermelons (fruits) from markets in Dar es Salaam, Tanzania. Journal of Applied Sciences and Environmental Management 21, 497–501. 11.1.17 Mahugija, J.A.M., Ahmed, K.N. and Makame, Y.M.M., 2017. Polycyclic aromatic hydrocarbons (PAHs) contamination in coastal mangrove ecosystems of the Zanzibar archipelago, Western Indian Ocean Journal of Marine Science 16(1), 25–34. 11.1.18 Mahugija, J.A.M., Nambela, L. and Mmochi, A.J., 2017. Levels and distribution of pesticide residues in soil and sediments in Eastern Lake Tanganyika environs, International Journal of Biological and Chemical Sciences 11(5), 2537–2547. 11.1.19 Mahugija, J.A.M., Chibura, P.E. and Lugwisha, E.H.J., 2018. Residues of pesticides and metabolites in chicken kidney, liver and muscle samples from poultry farms in Dar es Salaam and Pwani, Tanzania. Chemosphere 193, 869–874.

9 11.1.20 Mahugija, J.A.M., 2018. Levels of heavy metals in drinking water, cosmetics and fruit juices from selected areas in Dar es Salaam, Tanzania. Tanzania Journal of Science 44 (1), 1–11. 11.1.21 Mahugija, J.A.M. and Sheikh, H.M., 2018. Status of selected heavy metals dispersion from top soil in and around automobile workshop areas in Zanzibar Municipality, Tanzania. Tanzania Journal of Science 44 (1), 12-23. 11.1.22 Mahugija, J.A.M., Chibura, P.E. and Lugwisha, E.H.J., 2018. Occurrence of organochlorine and organophosphorus pesticide residues in poultry feeds, raw and cooked eggs from selected farms in Ilala and Kibaha Districts, Tanzania. Journal of Applied Sciences and Environmental Management 22(2), 191–196. 11.1.23 Mahugija, J.A.M., Nambela, L. and Mmochi, A.J., 2018. Determination of Dichlorodiphenyltrichloroethane (DDT) and metabolites residues in fish species from Eastern Lake Tanganyika. South African Journal of Chemistry 71, 86–93. 11.1.24 Mahugija, J.A.M., Kasenya, Z.S. and Kilulya, K.F., 2018. Levels of heavy metals in urine samples of school children from selected industrial and non-industrial areas in Dar es Salaam, Tanzania. African Health Sciences (Accepted, In Press). 11.1.25 Mahugija, J.A.M. and Njale, E., 2018. Levels of polycyclic aromatic hydrocarbons (PAHs) in smoked and sun-dried fish samples from areas in Lake Victoria in Mwanza, Tanzania. Journal of Food Composition and Analysis 73, 39-46, Elsevier. 11.1.26 Mahugija, J.A.M., 2018. Determination of toxic diethylene glycol in toothpastes in Dar es Salaam by high performance thin layer chromatography and colorimetric methods. Tanzania Journal of Science 44(2) special issue, 117-127. 11.1.27 Mahugija, J.A.M. and Njale, E., 2018. Effects of washing on the polycyclic aromatic hydrocarbons (PAHs) contents in smoked fish. Food Control 93, 139-143, Elsevier. 11.1.28 Mahugija, J.A. M., Kasenya, Z.S. and Kilulya, K.F. Variations of the concentrations of Pb, Zn, Fe, Cu and Cd in urine of primary school pupils in relation to age, sex and academic performance. Journal of Trace Elements in Medicine and Biology (Submitted 01 May 2018).

11.2 Publications in Conferences/Workshops Proceedings and Reports 1. J.A. Mahugija Marco, “Levels of pesticides and metabolites emanating from a point source: The case of Vikuge Farm, Kibaha, Tanzania”, MSc Thesis, University of Dar es Salaam, 2004. 2. J.A. Mahugija Marco and M.A. Kishimba, Levels of pesticides and metabolites in water, flora, soil and sediments due to point source contamination and the indications for public health risks– Proceedings of the African Network for the Chemical Analysis of Pesticides (ANCAP) Inaugural Conference, Arusha 2004, pp 79 – 88. 3. J.A. Mahugija Marco and M.A. Kishimba, Concentrations of polar pesticide residues in the surface waters of selected areas of Coast Region, Tanzania– A paper presented at the regional workshop on nitrogen, phosphorus and sulphur derivatives in the environment, Hotel Africana, Kampala–Uganda, 2005. 4. J.A. Mahugija Marco and M.A. Kishimba, Mango (Mangifera indica) leaves as biondicators of the spatial distribution of organochlorine pesticides and metabolites from a point source–A paper presented at the ANCAP Symposium, Makerere University, Uganda 21-22 July 2005. 5. J.A. Mahugija Marco, M.A. Kishimba and R. Joice, Organochlorine pesticides and metabolites in drinking water from sites near Vikuge storage site in Coast Region– Tanzania–A paper presented at the international conference on pesticide use in developing countries, Arusha International Conference Centre, 16th–20th October 2006. 6. J.A. Mahugija Marco and M.A. Kishimba, Comparison of the types and concentrations of organochlorine compounds in environmental samples between gas chromatographic- electron capture and mass spectrometric detection–A paper presented at the First

10 African Congress on Pesticides and Toxicology Sciences, held on 8th–11th November 2008 at the University of Gezira, Wad Medani, Gezira State-Sudan. 7. J.A. Mahugija Marco, “Chemodynamics of pesticides in soil at some hotspots in ten regions and in atmospheric matrices in Kibaha Tanzania”, PhD Thesis, University of Dar es Salaam, 2011. 8. J.A.M. Mahugija, Levels and toxicological implications of organochlorine pesticides and metabolites in soil at some hotspots in Tanzania–A paper presented at the 4th International Toxicology Symposium in Africa, held on 2nd–4th September 2012 at the University of Zambia, Lusaka. 9. L. Nambela and J.A.M Mahugija, Levels chemodynamics of pesticide residues in Lake Tanganyika Basin, Tanzania. A paper presented at the Sida-SAREC Regional Collaboration Conference, 31 July-2 August 2013, Bagamoyo-Tanzania. 10. J.A.M. Mahugija, B. Henkelmann and K.W. Schramm, Levels and patterns of organochlorine pesticides and their degradation products in rainwater in Coast region, Tanzania–A paper presented at the 6th SETAC Africa Conference held at Intercontinental Hotel in Lusaka, Zambia on 2nd–3rd September 2013 and at the 5th International Toxicology Symposium in Africa held on 11th–14th September 2013 at the Kwame Nkrumah University of Science and Technology in Kumasi, Ghana. 11. J.A.M. Mahugija, L. Nambela and A. Mmochi, Levels and distribution of DDT residues in fish species in eastern Lake Tanganyika in Tanzania–A paper presented at the 6th International Toxicology Symposium in Africa held on 28th–30th August 2014 at the Garden Court, O.R. Tambo International Airport in Johannesburg, South Africa. 12. J.A.M. Mahugija and L. Nambela, Pesticide residues in water from rivers and lakes in Lake Tanganyika basin, Tanzania–Peer Reviewed Proceedings of the 7th International Toxicology Symposium in Africa held at the Garden Court, O.R. Tambo International Airport in Johannesburg, South Africa, 31st August 2015, pp 77- 78. 13. J.A.M. Mahugija, Qualities of effective teaching and bad teaching in higher education - A Lecture presented at the Faculty Development Programme, St Joseph University in Tanzania, held at St Joseph College of Engineering Dar es Salaam, 6th October 2015. 14. J.A.M. Mahugija, F.A. Khamis and E.H.J. Lugwisha, Levels of pesticide residues in fruits and vegetables from markets in Dar es Salaam, Tanzania–Peer Reviewed Proceedings of the 8th International Toxicology Symposium in Africa held at Gawharet Al Ahram hotel in Giza-Cairo, Egypt, 29th–31st August 2016, pp 32–33. 15. E.H.J. Lugwisha, J.A.M. Mahugija and C. Mwankuna, Levels of formaldehyde and acetaldehyde in selected bottled drinking water sold in urban areas in Tanzania–A paper presented in the 9th International Toxicology Symposium in Africa held at the Constantial Hotel & Suites in Benin City, Nigeria, 07th–08th September 2017. 16. O. Ilomo and J.A.M. Mahugija, Collection and analysis of water samples from Export Processing Zones Authority (EPZA) and Twiga Cement Industry (Wazo) in Dar es Salaam: A consultancy (Registration No. UDSM-UCB/CoNAS 18-4) report submitted to the National Environment Management Council (NEMC), 27th April 2018. 17. K.F. Kilulya, J.A.M. Mahugija, O.O. Ilomo, S.S. Nyandoro, M.M. Ndoile, R. Peter, G.A. Kinunda, F.T. Sung'hwa, J.Y.N. Philip, E.H. J. Lugwisha, Evaluation of plastic bag industries and possibility of producing alternative bags in Tanzania–A consultancy (Registration No. UDSM-UCB/CoNAS/18-08) report submitted to National Environment Management Council (NEMC), Vice President’s Office, August 2018.

------02nd September 2018 Dr. John Andrew Marco Mahugija Senior Lecturer in Chemistry

11 CARRICULUM VITAE

1.0 PERSONAL PARTICULARS

Name Dr. Kessy Fidel Kilulya Nationality Tanzanian Date of Birth 10 th July, 1970 Marital status Married Department Chemistry College College of Natural and Applied Sciences (CoNAS) Address Chemistry Department, University of Dar es Salaam, P.O. Box 35061, Dar es Salaam, Tanzania, East Africa Fax (Office) +255-22-2410038 Mobile: +255-763-369858 and +255-676-807210 Email Address [email protected] or [email protected]

2.0 HIGHER EDUCATION

2012 PhD (Chemistry) ; University of Johannesburg, South Africa, Title of Thesis: “Profiling of Organic Extractives in Wood and Dissolving Pulping Process by Chromatographic and Spectroscopic Methods” 2005 MSc. (Chemistry) ; University of Dar es Salaam, Tanzania, Title of Thesis: “Evaluation of Patterns and Spatial Trends of Pesticide Residues Data from Selected Areas of Tanzania by Chemometric Methods” 1999 BSc. (Ed) Hons , Major subjects: Chemistry, Mathematics and Education, Class of Degree; Second Class - Upper Division; University of Dar es Salaam, Tanzania.

3.0 EMPLOYMENT AND WORKING EXPERIENCE

3.1 June 2012 -To Date : Lecturer, Chemistry Department, University of Dar es Salaam, Tanzania. . 3.2 November 2012 – October 2013 : Postdoctoral Research Fellow, University of Johannesburg, South Africa 3.3 2006 – June 2012 : Assistant Lecturer, University of Dar es Salaam, Chemistry Department. 3.4 2000 – 2002 : Part-time staff, Open University of Tanzania, Kilimanjaro Centre. 3.5 April – Oct. 2001 : Teacher, Majengo Secondary School, Moshi, Kilimanjaro.. 3.6 JULY, 1999 – April 2001 : Teacher and examination master, Kilimanjaro Academy, Moshi, Kilimanjaro.

4.0 ADMINISTRATIVE POSTS HELD AT THE UNIVERSITY OF DAR ES SALAAM

DURATION POST 01 ST AUGUST 2017 – TO DATE: Head, Chemistry Department. JULY 2016 – 30 TH July 2017: Acting Head, Chemistry Department. MAY 2016 – TO DATE: Acting Manager of Natural Science Consultancy Bureau (NSCoB). .JULY 2015 – TO DATE: Board Member, College of Natural and Applied Sciences (CoNAS). JULY 2015 – AUG. 2016: Coordinator of Knowledge Exchange and Consultancy, College of Natural and Applied Sciences (CoNAS), JULY 2015 – TO DATE: Member of the Departmental Resource Mobilization and Consultancy Committee, Chemistry Department 2014 – TO DATE: Member of the Departmental HDRP committee, Chemistry Department FEB - SEP. 2008: Departmental Examination Officer.

5.0 PROFESSIONAL AFFILIATION

5.1 Member, Tanzania Chemical Society (TCS) 5.2 Member, The South African Chemical Institute (SACI)

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5.3 Member, Society of Environmental Toxicology and Chemistry (SETAC)

6.0 RESEARCH AREAS AND CURRENT RESEARCH 6.1 Analysis of chemical cellulose contaminants (i.e. lipophilic extractives) and their effects on the quality of chemical cellulose. 6.2 Preparation, characterization and applications of molecularly imprinted polymers (MIP) and other adsorbents for selective removal of water contaminants. 6.3 Investigation of biomolecule indicators and lipophilic extractives in blue-green algae in relation to biotoxic metabolite production and contamination of freshwater bodies. 6.4 Investigation of the levels of aflatoxins in maize and maize products from selected Regions of Tanzania 6.5 Investigation of the levels of aflatoxins in spices produced in selected Regions of Tanzania (i.e., Zanzibar, Dar es Salaam and Arusha). 6.6 Determination of levels of heavy metals in urine among Primary School Pupils living in selected industrial and non-industrial areas in Dar es Salaam. 6.7 Determination of heavy metals in water, sediments and biota in freshwater bodies. 6.8 Chemical Quality Control and Flow Assurance in Oil and Gas Systems in Tanzania. 6.9 Isolation, Modification and Performance of Bio-based polymeric materials for Chemical Enhanced Oil and Gas Recovery.

7.0 AWARDS and GRANDS 7.1 CSIR funding for the PhD project, September, 2008 to December, 2011; South Africa 7.2 University of Johannesburg Postdoctoral Scholarship Award, Nov. 2012 to October 2013, South Africa. 7.3 A collaborating member in the consortium for Tanzania-Netherland energy project funded by NUFFIC, Netherlands through NICHE Project; 2016 – 2019.

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7.4 A collaborating member in the consortium which is currently running an Africa Centre of Excellence for Water Management (ACEWM) hosted by the University of Addis Ababa, Ethiopia, funded by World Bank; 2016 - 2020. 7.5 EU Tanzania Capacity Building project, scoping research projects Tranche 2; 2017 – 2018; €15,000. A joint project between UDSM and Aberdeen University

8.0 CONFERENCES, WORKSHOPS AND COURSES ATTENDED 8.1 October, 2001 – May 2002 : Training in Multivariate Data Analysis and Design of Experiments, University of Dar es Salaam. 8.2 June, 2004 : Training in Customer Care, University of Dar es Salaam. 8.3 12 th -15 th October, 2006 : Training in Cyberchemistry (Sharing Instrumentation Globally), Pesticide Registration and the Use of Multivariate Techniques for Analyzing Ecological and Ecotoxicological Data. In the 5 th ANCAP Summer School held in TPRI, Arusha, Tanzania. 8.4 16 th – 20 th October, 2006 : Participated in the International Conference on Pesticide Use in Developing Countries: Environmental Fate, Effects and Public Health Implications. Arusha International Conference Centre, Arusha, Tanzania. 8.5 20 th October, 2006 : Training in Pesticide Use, Occupational Health Risks, and Environmental Risk Assessment Procedures to Protect Surface Waters (with emphasis on extrapolation between temperate and tropical systems). Arusha International Conference Centre, Arusha, Tanzania. 8.6 21 st – 22 nd October, 2006 : Participated in the IFS Workshop on the development of scientific projects. TPRI, Arusha, Tanzania. 8.7 4TH – 5TH July, 2009 : Attended Southern and Eastern Africa Network of Analytical Chemists (SEANAC) pre-conference workshop on sample preparation for HPLC, sample preparation for GC and Biosensors in Food and Agriculture, held at the University of Swaziland, Manzini, Swaziland. 8.8 5TH - 8TH July, 2009 ; Participated in SEANAC International Conference on Chemistry for Food Security and Sustainable Development, Manzini, Swaziland. 8.9 5TH – 9TH December. 2010: Participated in Analitika 2010 International Conference on Analytical Sciences; Stellenbosch University; South Africa.

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8.10 5TH – 7TH October, 2011: Participated in “The 2nd Tanzania Chemical Society (TCS) International Conference’ Chemistry for the Improvement of Life”; Dar es Salaam, Tanzania. 8.11 26 TH – 28 TH October, 2011: Participated in “The 12th WaterNet/WARFSA/GWPSA Symposium”; Maputo, Mozambique. 8.12 1ST November 2012 – 31 ST October 2013 : Postdoctoral Research Fellow, University of Johannesburg, South Africa. 8.13 30 TH October – 1ST November 2013 ; Participated in “The 14th WaterNet/WARFSA/GWPSA Symposium”; Whitesands Hotel, Dar es Salaam, Tanzania. 8.14 02 nd - 03 rd May, 2016: Training in Technology and Innovation Support Center (TISC): Training of Trainers’ Workshop on the Effective Use of Technical and Scientific Information. Organized by the World Intellectual Property Organization (WIPO). Held at COSTECH Dar es Salaam, Tanzania. Awarded Certificate. 8.15 30 th May – 03 rd June, 2016 , Training in Innovation Management by Entrepreneurship (TIME), University of Dar es Salaam, Tanzania. Awarded Certificate. 8:16 20 th February – 03 rd March, 2017 , Capacity Training in Entrepreneurial Rural Innovation and Growth. University of Dar es Salaam, Tanzania. Awarded Certificate. 8.17 11 TH – 15 TH September, 2017: Participated in “The 3 rd Tanzania Chemical Society (TCS) International Conference and 6 th FASC Congress, Chemistry for Improvement Livelihood”; Naura Springs Hotel, Arusha, Tanzania.

9.0 PUBLICATIONS

9.1 JOURNAL PAPERS

9.1.1 Kessy F. Kilulya , Titus A. M. Msagati, Bhekie B. Mamba, J. Catherine Ngila and Tamara Bush. “Imidazolium Ionic Liquids as Dissolving Solvents for Chemical- Grade Cellulose in the Determination of Fatty Acids Using Gas Chromatography Mass Spectrometry” BioResources , 6(3), ( 2011 ), 3272-3288.

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9.1.2 Kessy F. Kilulya , Titus A. M. Msagati, Bhekie B. Mamba, J, Catherine Ngila and Tamara Bush. Study of the fate of lipophilic wood extractives during acid-sulphite pulping process by ultrasonic solid-liquid extraction and gas chromatography mass spectrometry. Journal of Wood Chemistry and Technology, 32 (2012 ) 253- 267. 9.1.3 Kessy F. Kilulya , Titus A. M. Msagati, Bhekie B. Mamba, J, Catherine Ngila and Tamara Bush. Ionic liquid-liquid extraction and supported liquid membrane analysis of lipophilic wood extractives from dissolving pulp. Chromatographia, 75 ( 2012 ) 513-520. 9.1.4 K. F. Kilulya , T. A. Msagati, B. B. Mamba, J. C. Ngila and T. Bush, Controlling the release of wood extractives into water bodies by selecting suitable Eucalyptus species. Journal of Physics and Chemistry of the Earth, (2012), Parts A/B/C 50– 52:217-223. 9.1.5 KF Kilulya , TAM Msagati, BB Mamba, JC Ngila and T Bush, Determination of Lipophilic Extractives in Ionic Liquid Extracts of Eucalyptus Pulp by Gas Chromatography - Mass Spectrometry. Tanzania Journal of Science , ( 2012 ), 38(3), 14-26. 9.1.6 KF Kilulya and GS Mhinzi, Evaluation of Patterns and Spatial Trends of Pesticide Residues from Vikuge Farm, Coast Region, Tanzania by Principal Components Analysis. Tanzania Journal of Science , ( 2012 ), 38(3), 184-200. 9.1.7 Kessy F. Kilulya , Titus A.M. Msagati and Bhekie B. Mamba Ionic Liquid-Based Extraction of Fatty Acids from Blue- Green Algal Cells Enhanced by Direct Transesterification and Determination Using GC × GC-TOFMS, Chromatographia, , ( 2014 ), 77, 479 – 486. 9.1.8 Kessy F. Kilulya , Titus A.M. Msagati, Bhekie B. Mamba, J. Catherine Ngila and Tamara Bush, Effect of Site, Species and Tree Size on the Quantitative Variation of Lipophilic Extractives in Eucalyptus Woods Used for Pulping in South Africa, Industrial Crops and Products, (2014 ), 56, 166 – 174. 9.1.9 D. T. Maiga, T. A. M. Msagati, K. F. Kilulya and B. B. Mamba; Preparation of SPE hybrid mesoporous silica sorbents for the analysis and removal of organic

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pollutants in water, Journal of Physics and Chemistry of the Earth ( 2014 ), Parts A/B/C,72 – 75; 83 – 87. 9.1.10 Joseph Sempombe, Veronica Mugoyela, Matobola Joel Mihale, Abdallah Zacharia1, Pius Ipagala and Kessy Fidel Kilulya ; Preliminary in vivo antitrypanosomal activity and cytotoxicity of Entada abyssinica, Securinega virosa and Ehretia amoena; East and Central African Journal of Pharmaceutical Sciences; Vol. 17 ( 2014 ) 37-43. 9.1.11. Geovin Mgoyela, Joseph Sempombe , Kessy Fidel Kilulya , Mhina Chambuso , Veronica Mugoyela and Eliangiringa Kaale; A Quantitave Near Infrared Spectroscopy Model for the Assay of Efavirenz in Tablets; East and Central African Journal of Pharmaceutical Sciences; Vol. 17 ( 2014 ), 54-62 . 9.1.12. Kessy F. Kilulya , Titus A. M. Msagati, Bhekie B. Mamba. Book Title: “Ionic Liquids – Current State of the Art”; Chapter title (Chapter 13): Imidazolium- Based Ionic Liquids as Solvents for Analysis of Lipophilic Extractives from Biomass. Ionic Liquids; Book edited by: Dr. Scott Handy, ISBN 978-953-51- 2122-0, InTech, (2015), 351 – 364. http://dx.doi.org/10.5772/59075. 9.1.13. Kessy F Kilulya , Bhekie B Mamba and Titus AM Msagati; Extraction Procedures and GCxGC-TOFMS Determination of Fatty Acids (FAs) in Cyanobacteria Cultures and the Effect of Growth Media Iron Concentration Variation on Cellular FAs Composition; Journal of Environmental and Analytical Toxicology, ( 2015 ), S7:S7-009, doi:10.4172/2161-0525.S7-009. 9.1.14. Kessy F. Kilulya , Titus A.M. Msagati, Bhekie B. Mamba, J. Catherine Ngila and Tamara Bush, Evaluation of the Influence of Lipophilic Extractives on Dissolving Pulp Quality Parameters by Partial Least Squares Method of Chemometrics, NPPRJ, Volume 30, Issue No. 3, (2015 ) 402-410 . 9.1.15. Kessy F. Kilulya , Titus A.M. Msagati and Bhekie B. Mamba, Effect of Natural Blue-Green Algal Cells Lysis on Freshwater Quality. Tanzania Journal of Science , ( 2017), 43(10), 83 – 94. 9.1.16. Kessy F. Kilulya , Preparation, Characterisation and Application of Molecularly Imprinted Polymers for the Selective Removal of Sterols from Water. Tanzania Journal of Science (2018), 44(2), 27-44.

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9.1.17. Faustin N Ngassapa, Othman C Othman, David Mihayo and Kessy F. Kilulya , Speciation of Lead, Chromium, Cadmium, Copper and Zinc in Sediments and Plants along Msimbazi River in Dar es Salaam, Tanzania. Tanzania Journal of Science , (2018). 44(3), 136 – 151.

9.2 PAPERS IN CONFERENCES AND WORKSHOPS

9.2.1 K. Fidel and G. S. Mhinzi, “Evaluation of Patterns and Spatial Trends of Pesticide Residues Data from Vikuge Farm, Coast Region, Tanzania by Chemometric Methods”. A paper presented at “International Conference on Pesticide Use in Developing Countries: Environmental Fate, Effects and Public Health Implications” 16 th -20 th October, 2006. AICC, Arusha, Tanzania. 9.2.2 K. F. Kilulya, T. A. M. Msagati and J. C. Ngila; “The Application of Hollow Fibre Supported Liquid Membranes (HFSLM) in the Extraction of Agrochemical Residues from Water Samples”. A poster presented at “International Conference on Chemistry for Food Security and Sustainable Development” 5 th - 8th July, 2009. Manzini, Swaziland. 9.2.3 Kessy Fidel Kilulya , Titus Msagati, Bhekie B. Mamba, Catherine Ngila and Tamara Bush; “Assessment of imidazolium-based room temperature ionic liquids as solvents for dissolving pulp and the determination of fatty acids in the solution using gas chromatography-mass spectrometry”. A paper presented at ‘Analitika 2010 Conference’ International conference on analytical sciences’; 5 th – 9th December, 2010. Stellenbosch University, South Africa. 9.2.4 Kessy Fidel Kilulya , Titus A. M. Msagati, Bhekie B. Mamba, J. Catherine Ngila and Tamara Bush; “Determination of lipophilic extractives in ionic liquid extracts of eucalyptus pulp by gas chromatography-electron ionization-mass spectrometry”. A paper presented at ‘The 2 nd Tanzania Chemical Society (TCS) International Conference’ Chemistry for the Improvement of Life’; 5 th – 7th October, 2011. Dar es Salaam, Tanzania. 9.2.5 Kessy Fidel Kilulya, T. A. Msagati, B. B. Mamba, J. C. Ngila and T. Bush, “Control Of Extractives Release into Water Bodies from Pulping by Selecting Suitable Eucalyptus Species”. A paper presented at the 12 th

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WaterNet/WARFSA/GWP-SA Symposium; 26 th – 28 th October, 2011, Maputo, Mozambique. 9.2.6 Kessy F. Kilulya, Titus A.M. Msagati and Bhekie B. Mamba, Effect of Natural Blue-Green Algal Cell Lysis on Water Quality. A paper presented at the 14 th WaterNet/WARFSA/GWP-SA Symposium; 30 th October – 01 st November, 2013, Whitesands Hotel, Dar es Salaam, Tanzania. 9.2.6 Kessy F. Kilulya , Preparation, Characterisation and Application of Molecularly Imprinted Polymers for the Selective Removal of Sterols from Water. A paper presented at ‘The 3 rd Tanzania Chemical Society (TCS) International Conference and 6 th FASC Congress’ Chemistry for Improvement Livelihhod; 11 th – 15 th September, 2017; Naura Springs Hotel, Arusha, Tanzania.

10.0 EXPERIENCE AS EXTERNAL AND INTERNAL EXAMINER 10.1 Geovin George Mgoyela , M.Sc. Dissertation. Near Infrared Spectroscopy as a Field Adapted Technology for Drugs Quality Assessment. Muhimbili University of Health and Allied Sciences – MUHAS (2013 ). 10.2 David Mihayo , M.Sc. Dissertation. Speciation of Heavy Metals in Plants, Water, and Sediments along Msimbazi River in Dar es Salaam, Tanzania. University of Dar es Salaam – UDSM ( 2014). 10.3 Petro Ezekiel Mabeyo , M.Sc. Dissertation. Selenium Bioaccumulation in Leafy Vegetables as Potential Nutraceuticals. University of Dar es Salaam – UDSM (2014). 10.4 Auguster Kayombo , M.Sc. Dissertation. Assessment of Pesticides Residues Levels in Raw and Processed Maize Grains and Flour from Selected Areas in Dar es Salaam and Ruvuma. University of Dar es Salaam – UDSM ( 2014). 10.5 Silvia John , M.Sc. Dissertation. Levels of Heavy Metals in Selected Facial Cosmetics Marketed in Dar es Salaam, Tanzania. University of Dar es Salaam – UDSM ( 2015). 10.6 Mohamed Salum , M.Sc. Dissertation. Aflatoxin Levels in Sunflower Seeds, Feeds, Seed Cakes, Oil and Cow Milk Collected from Singida. University of Dar es Salaam – UDSM ( 2015).

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10.7 Patrick Edward Chibura , M.Sc. Dissertation. Levels of Pesticides Residues in Poultry Feed and Products in Selected Poultry Farms in Ilala and Kibaha Districts, Tanzania. University of Dar es Salaam – UDSM ( 2016 ). 10.8 Fides Simon , M.Sc. Dissertation. Risk of Heavy Metals Exposure through Consumption of Rice from Kahama and Geita Districts. The Nelson Mandela African Institution of Science and Technology - NM-AIST ( 2016 ). 10.9 Dhanasagree Sepuru , M.Sc. Dissertation. Carbon Monoxide Analysis of Post Mortem Blood Samples. University of South Africa (UNISA) ( 2017 ). 10.10 Kazula Henry , M.Sc. Dissertation. Sythesis of Mesoporous Carbon from Non- biodegradable Plastic Bags for Water Purification: Methylene Blue Removal. The Nelson Mandela African Institution of Science and Technology - NM-AIST ( 2017) 10.11 Blasius Ngayakamo , M.Sc. Dissertation. Evaluation of Dielectric and Mechanical Strength of High Voltage Porcelain Insulators Made from Tanzania Ceramic Materials. The Nelson Mandela African Institution of Science and Technology - NM-AIST ( 2018) 11.0 TEACHING AND SUPERVISION AT THE UNIVERSITY OF DAR ES SALAAM:

11.1 TEACHING

Undergraduate courses CH 248: Instrumental Methods in Analytical Chemistry CH 371: Quality Control and Assurance CH 314: Project Work (Supervision) Postgraduate (M.Sc. & PhD) courses CH 630: Advanced Analytical Chemistry and Instrumentation CH 658: Analytical Biochemistry EV 718: Methods in Environmental Chemical Analysis

11.2 SUPERVISION OF POSTGRADUATE STUDENTS

S/N Name Topic and Status 1 Macarius P. Levels of Aflatoxins in Maize and Maize Products from Mtega Selected Regions of Tanzania ( M.Sc. Completed ) 2 Zainab S. Determination of Levels of Heavy Metals in Urine among Kasenya Primary School Pupils Living in Selected Industrial and Non Industrial Areas in Dar es Salaam ( M.Sc. Completed )

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3 Khadija Salum Investigation of Levels of Aflatoxins in Spices Produced in Ali Selected Regions of Tanzania (i.e., Zanzibar and Dar es Salaam) (M.Sc. Completed ) 4 Geofrey, The Study of the Fate of Selected Antibiotics and their Kapesa Metabolites in Chicken from Dar es Salaam Poultries (M.Sc. Ongoing ) 5 Sharifa Juma Investigation of Levels of Aflatoxins in Selected Spices Produced and Marketed in Selected Locations of Tanzania (M.Sc. Ongoing ) 6 Gobry, Investigation on Spatial and Seasonal Variations in the Josephine John Levels of Selected Emerging Contaminants Occurring in Some Tanzanian Freshwater Bodies (PhD. Ongoing ) 7 Augustino, Isolation, Modification and Performance of Bio-based Oliva polymeric materials for Chemical Enhanced Oil and Gas Recovery (M.Sc. Ongoing ) 8 Annastazia N. Optimization of Sample Preparation, Detection and Wandwi Quantification of Selected Drugs of Abuse and Metabolites in Urine Samples using LC-MS/MS (M.Sc. Ongoing ) 9 Phares M. D. A Study on Levels and Distribution of Selected Heavy Petro Metals in Soil, Maize and Rice within the Locality of Buzwagi Gold Mine, Kahama, Tanzania (M.Sc. Ongoing ) 11 Gwae Investigations on the Levels of Selected Heavy Metals in the Emmanuel Vicinity of Dar es Salaam City and Tracking their Sources either Natural or Anthropogenic Based on their Stable Isotopes (PhD. Ongoing )

12.0 CONSULTANCY

12.1 November 2016 ; Investigation on the Type and Quality of Imported Palm oil; (Client TRA) 12.2 February – March 2018 : Training of NEMC staff on Environmental Sampling and Analysis 12.3 March 2018 : Training of SUA technical Staff on Atomic Absorption Spectroscopy

13.0 OTHER ACTIVITIES AND EXTRA CURRICULAR

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13.1 Organizing Committee Treasurer for the 3 rd Tanzania Chemical Society (TCS) International Conference and the 6 th Federal of African Societies of Chemistry (FASC) – 11 th – 15 th September, 2017; Tanzania. 13.2 Secretary of Tanzania Chemical Society (TCS) from 11 th November 2017 – To Date. 13:3 Member, Board of Directors of Education Insight for All from 05 Th December, 2017 – To Date.

SIGNED ...... Date…11 TH JUNE, 2018... Dr. Kessy Fidel Kilulya, Lecturer of Analytical and Environmental Chemistry

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