Generation of a Whole-Brain Atlas for the Cholinergic System and Mesoscopic Projectome Analysis of Basal Forebrain Cholinergic Neurons
Generation of a whole-brain atlas for the cholinergic system and mesoscopic projectome analysis of basal forebrain cholinergic neurons Xiangning Lia,b,1, Bin Yuc,d,1, Qingtao Suna, Yalun Zhanga, Miao Rena, Xiaoyan Zhanga, Anan Lia,b, Jing Yuana, Linda Madisene, Qingming Luoa,b, Hongkui Zenge, Hui Gonga,b,2, and Zilong Qiub,c,2 aBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, School of Engineering Sciences, Key Laboratory of Biomedical Photonics of Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, China; bChinese Academy of Sciences Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai 200031, China; cInstitute of Neuroscience, State Key Laboratory of Neuroscience, Chinese Academy of Sciences, Shanghai 200031, China; dUniversity of Chinese Academy of Sciences, Beijing 100049, China; and eAllen Institute for Brain Science, Seattle, Washington 98103 Edited by Liqun Luo, Howard Hughes Medical Institute and Department of Biology, Stanford University, Stanford, CA 94305, and approved November 21, 2017 (received for review March 6, 2017) The cholinergic system in the brain plays crucial roles in regulating continuous sectioning was recently developed to obtain whole- sensory and motor functions as well as cognitive behaviors by brain maps for neurons of interest (13–16). Using the latest modulating neuronal activity. Understanding the organization of tomography-based method (15), we constructed a comprehensive the cholinergic system requires a complete map of cholinergic whole-brain map of genetically labeled cholinergic neurons in the neurons and their axon arborizations throughout the entire brain mouse brain, which provides quantitative information on neuronal at the level of single neurons.
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