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(12) Patent Application Publication (10) Pub. No.: US 2013/0045934 A1 Tsuji Et Al

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(12) Patent Application Publication (10) Pub. No.: US 2013/0045934 A1 Tsuji Et Al US 2013 0045934A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2013/0045934 A1 Tsuji et al. (43) Pub. Date: Feb. 21, 2013 (54) EXTRACTION METHOD USING ULTRA FINE A2.3L I/28 (2006.01) BUBBLES AND LIQUID EXTRACTS A6IP 25/20 (2006.01) OBTANED THEREOF A6IP35/04 (2006.01) (75) Inventors: Hideyasu Tsuji, Fukuyama-shi (JP); A6IP35/00 (2006.01) Yasuhiro Tsuji, Fukuyama-shi (JP); A63L/235 (2006.01) Toru Oka, Takatsuki-shi (JP); Haruka A6IP3/08 (2006.01) Miyao,Liauw, Takatsuki-shiSingapore (SG) (JP); Denny (52) U.S. Cl. ........... 514/25: 514/533; 426/431; 426/655 (73) Assignees: Sunstar Engineering Inc., Takatsuki-shi (57) ABSTRACT (JP); Ligaric Co., Ltd., Suita-shi (JP) The present invention provides an extraction method that (21) Appl. No.: 13/583,367 either does not use emulsifiers, organic solvents, and the like (22) PCT Filed: Mar. 3, 2011 or can reduce the amount used of emulsifiers, organic Sol vents, and the like. In addition, the extraction method of (86). PCT No.: PCT/UP2011/O54903 present invention can also efficiently extract various compo S371 (c)(1), nents from various materials to be extracted, and can highly (2), (4) Date: Nov. 5, 2012 maintain potency and the like of the extracted components, in particular if the components are active agents. Furthermore, (30) Foreign Application Priority Data the extraction method of present invention has excellent Mar. 8, 2010 (JP) ................................. 2010-051.146 safety. The extraction method of present invention is achieved by bringing materials to be extracted into contact with liquid Publication Classification containing ultra fine bubbles for extraction treatment. The (51) Int. Cl. ultra fine bubbles utilized during the extraction process pref A6K 3L/7024 (2006.01) erably have a mode diameter of 500 nm or smaller and a A6IP37/08 (2006.01) concentration of 1,000,000 or more per 1 ml of liquid. US 2013/0045934 A1 Feb. 21, 2013 EXTRACTION METHOD USING ULTRA FINE SUMMARY OF INVENTION BUBBLES AND LIQUID EXTRACTS OBTANED THEREOF Technical Problem 0010. In view of the above problems, an object of the TECHNICAL FIELD present invention is to provide an extraction method that does not use emulsifiers, organic solvents, and the like or can 0001. The present invention relates to an extraction reduce the amount used of emulsifiers, organic solvents, and method using ultra fine bubbles and the liquid extract the like, can efficiently extract various components from vari obtained by the extraction method. ous materials to be extracted, can highly maintain potency and the like of an extracted component when the component BACKGROUND ART is an active component, and further has excellent safety; and 0002 Conventionally, in order to extract a desired compo liquid extract obtained by the extraction method. nent from a material to be extracted, for example, an aqueous Solvent or an organic solvent has been used to extract a com Solution to Problems ponent soluble in each solvent, thereby obtaining the compo 0011. The present inventors have carried out intensive nent as liquid extract (extract). studies in order to solve the above problems, as a result, have 0003 For example, when a plant material is used as the found that, by using water containing ultra fine bubbles, vari material to be extracted to obtain its extract, in many cases, an ous components can be efficiently extracted from various extraction solvent including water, organic solvents such as materials to be extracted without using emulsifiers, organic methanol, ethanol, butanol, isopropyl alcohol, hexane, hep Solvents, and the like; or using emulsifiers, organic solvents, tane, cyclohexane, ethyl acetate, acetone, and petroleum and the like in reduced amount, and the invention has been ether, polyols such as 1,3-butylene glycol, glycerin, and pro accomplished. pylene glycol, and oils and mineral oils such as castor oil, 0012. The summary of the present invention is as follows. olive oil, Sunflower seed oil, squalene, and liquid paraffin is 0013 (1) The present invention relates to an extraction singly used or these extraction solvents are appropriately method including bringing materials to be extracted into con mixed to be used, a raw or dried plant is subjected to or not tact with water containing ultra fine bubbles for extraction Subjected to treatment such as pulverization and fine cutting treatment. and then is subjected to, for example, cold immersion, warm 0014 (2) The present invention relates to the extraction immersion, heating and refluxing, or percolation to extract method in which the ultra fine bubbles have a mode diameter soluble components. of 500 nm or smaller. 0004. However, there are some problems in such an 00.15 (3) The present invention relates to the extraction extraction method from the viewpoints of potency and qual method in which 1 mL of the water contains 1,000,000 or ity. For example, a conventional steam distillation needs heat more of the ultra fine bubbles. ing or solvent extraction after the distillation and this may 0016 (4) The present invention relates to the extraction alter obtained components or may leave solvent which may method in which the ultra fine bubbles have an electrically cause irritating Smell. From the viewpoint of the production, charged Surface and the Surface has an absolute Zeta potential a solvent is required in a relatively large amount and Such a of 5 mV or more. solvent has to be removed, for example. In addition, hydro 0017 (5) The present invention relates to the extraction philic components cannot be extracted. method in which the ultra fine bubbles substantially include 0005. By extraction methods using water, the amount of one or more gases selected from air, oxygen, hydrogen, nitro extracted components is Small and extractable components gen, carbon dioxide, oZone, and inert gases. In the present are limited. On this account, an extraction method using a invention, the inert gases mean noble gases Such as helium, mixture with a hydrophilic solvent (Patent Document 1) and neon, and argon. a method by adding an emulsifier in a small amount (Patent 0018 (6) The present invention relates to the extraction Document 2) have been developed. However, such methods method in which the water contains water-soluble solvents in may leave a solvent or an additive, and may raise problems an amount of 8% by weight or less. Such as limited applications of the extract and safety issue. A 0019 (7) The present invention relates to the extraction method of obtaining an extract by Supercritical extraction method in which the material to be extracted is at least one using carbon dioxide gas in a Supercritical state (Patent Docu selected from animal materials, a plant materials, soils, and an ment 3) has also been developed, but has problems of, for OS. example, the cost and apparatus. 0020 (8) The present invention relates to the extraction 0006. The extraction using animal materials, soils, and method further including applying one or more waves ores as materials to be extracted also has Substantially the selected from electromagnetic waves, Sound waves, and same problems as above. ultrasonic waves as the extraction treatment. 0021 (9) The present invention relates to the extraction method in which the ultra fine bubbles are generated using a CITATION LIST ultra fine bubble generating apparatus equipped with a gas liquid shearing mixer. Patent Literatures 0022 (10) The present invention relates to the extraction method in which the materials-to-be extracted are firstly 0007 Patent Document 1: JP-A No. 10-202002 immersed in water, and the ultra fine bubbles are simulta 0008 Patent Document 2: JP-A No. 2002-84992 neously generated in this materials-to-be extracted contain 0009 Patent Document 3: JP-A No. 2000-237501 ing liquid by ultra-fine bubbles generating apparatus. US 2013/0045934 A1 Feb. 21, 2013 0023 (11) The present invention relates to liquid extract analysis system, NanoSight series (manufactured by Nano obtained by the extraction method as described in any one of Sight). The nanoparticle analysis system, NanoSight series the items (1) to (10). (manufactured by NanoSight) measures the speed of Brown 0024 (12) The present invention relates to an extract pow ian motion of nanoparticles and calculates the particle size der obtained by drying the liquid extract as describe in the and the particle number based on the speed. The system can item (11). also determine the size and the number of bubbles in a similar 0025 (13) The present invention relates to a composition manner. The concentration and mode diameter of the bubbles containing the liquid extract as described in the item (11) or can be ascertained by the particle size distribution of nano the extract powder described in the item (12). particles measured. 0031. In the present invention, the ultra fine bubbles pref Benefits of the Invention erably have an electrically charged surface and the Surface preferably has an absolute Zeta potential of 5 mV or more and 0026. According to the extraction method of the present more preferably 7 mV or more. It is supposed that ultra fine invention, various components can be efficiently extracted bubbles having a surface with a Zeta potential within such a from various materials to be extracted without using emulsi range enhance the stabilization of an extraction component fiers, organic solvents, and the like; or with emulsifiers, due to a surface activation effect derived from the Zeta poten organic solvents, and the like in a reduced amount. The tial on the bubble surface. In particular, when components are method can highly maintain the potency and the like of an extracted from animal or plant materials, Surface hydropho extracted component when the component is an active com bicity of ultra fine bubbles increases the permeability effi ponent.
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