Pholiotina Longistipitata Fungal Planet Description Sheets 361

360 Persoonia – Volume 38, 2017

Pholiotina longistipitata Fungal Planet description sheets 361

Fungal Planet 614 – 20 June 2017 Pholiotina longistipitata E.F. Malysheva & Kiyashko, sp. nov.

Etymology. The epithet emphasises the important character of the new Habitat & Distribution — In a small group, on litter in mixed species – basidiocarps with long stipes. forest. Up to now known only from the type locality.

Classification — Bolbitiaceae, Agaricales, Agaricomycetes. Typus. Russia, Krasnoyarsk Territory, Sayano-Shushenskiy State Bio- spheric Nature Reserve, floodplain of Malaya Golaya River, mixed forest Pileus 5–17 mm, broadly campanulate or obtuse conical, with- (Abies sibirica, Pinus sibirica, Betula pendula), among moss, 17 Aug. 2015, out distinct umbo, with even margin; hygrophanous, up to A. Kiyashko & E. Malysheva (holotype LE312984, ITS and LSU sequences centre striate; pale brownish orange (7C3–4), sometimes with GenBank KY627842 and KY627843, MycoBank MB819993). greyish tint, towards margin paler to cream, towards centre Notes — Pholiotina longistipitata is characterised by the fol- darker – agate or henna (7CE) to reddish brown (8E8) (co- lowing features: rather slender basidiocarps with conical pilei lour terms according to Kornerup & Wanscher 1978); surface strongly striated up to the centre, long whitish stipes, relatively smooth. Lamellae moderately distant, narrowly adnate, hardly small elongate-ellipsoid basidiospores, and numerous pileo- ventricose, orange-brown to yellow-brown, with concolorous cystidia in the pileus. edge. Stipe 50–110 × 1–2 mm, cylindrical, subbulbous base; entirely pure white or slightly yellowish; longitudinally fibrillose, Due to absence of a veil and lageniform cheilocystidia, Pholio minutely pruinose or almost smooth; veil absent. Basidiospores tina longistipitata can be placed in sect. Piliferae. This new 8–9.5 × 4.3–5.4 µm, Q = (1.23–)1.35–2.00, Q* = 1.72, nar- species is quite similar to Ph. striipes on the basis of a com- rowly to broadly ellipsoid, elongate-ellipsoid, yellow-brown in plex of microscopic features, but the latter noticeably differs in KOH, slightly thick-walled, with distinct germ pore. Basidia habit, forming rather stout basidiocarps that commonly grow 18–27 × 8–10 µm, 4-spored, broadly clavate. Cheilocystidia in fascicles, having a lower ratio of stipe length to pileus dia 27–55 × 6–11 µm, narrowly to broadly lageniform, fusiform with meter as well as differently shaped and weakly striated pileus inflated base and obtuse, occasionally bifurcated, apex, some (Hausknecht 2009). An additional difference is based on an ITS proportion utriform, thin- or slightly thick-walled. Pileipellis a sequence analysis which demonstrated strong dissimilarity with hymeniderm, consisting of clavate to sphaeropedunculate ele- more than 30 % distance between sequences (in comparison ments, 23–50 × 15–25 µm, slightly thick-walled. Pileocystidia with WU269997 specimen of Ph. striipes originated from Aus- numerous, similar to cheilocystidia but larger (up to 110 µm long tria). Pholiotina pygmaeoaffinis differs in having significantly and 20 µm wide), often pigmented and thick-walled. Caulocys- larger basidiospores, smaller caulocystidia and geographical tidia numerous, often in clusters, similar to cheilocystidia, but distribution restricted by Europe (Hausknecht 2009). larger and more often irregular-shaped, 45–110 × 8–17 µm. Clamp connections present.

Best tree from a ML analysis of the ITS dataset of some Pho- liotina species from sect. Piliferae. Support values (BS ≥ 50 % / PP ≥ 0.95) are given above the branches. Maximum likeli- hood analysis was run in the PhyML server v. 3.0 (http://www. atgc-montpellier.fr/phyml/) under a GTR model, with 100 rapid bootstrap replicates. Bayesian analysis was performed with MrBayes v. 3.1 software (Ronquist & Huelsenbeck 2003) for two independent runs, each with 5 million generations and under the same model. Taxa names are followed by the GenBank ac- cession numbers. The novel species is indicated in bold. The tree was rooted to Conocybe apala (GenBank JX968209). The Colour illustrations. Russia, South Siberia, Sayano-Shushenskiy State Biospheric Nature Reserve, site in taiga where fungus was found; basidio scale bar indicates the expected changes per site. carps, basidiospores, cheilocystidia, caulocystidia (all from holotype). Scale bars = 1 cm (basidiocarps), 10 µm (microscopic structures).

Ekaterina F. Malysheva & Anna A. Kiyashko, Komarov Botanical Institute of the Russian Academy of Sciences, Saint Petersburg, Russia; e-mail: [email protected] & [email protected]