Table 6. Putative genes involved in the utilization of carbohydrates in G. thermodenitrificans NG80-2 genome Carbohydrates* Enzymes Gene ID Glycerol Glycerol Kinase GT1216 Glycerol-3-phosphate dehydrogenase, aerobic GT2089 NAD(P)H-dependent glycerol-3-phosphate dehydrogenase GT2153 Enolase GT3003 2,3-bisphosphoglycerate-independentphosphoglycerate mutase GT3004 Triosephosphate isomerase GT3005 3-phosphoglycerate kinase GT3006 Glyceraldehyde-3-phosphate dehydrogenase GT3007 Phosphoglycerate mutase GT1326 Pyruvate kinase GT2663 L-Arabinose L-arabinose isomerase GT1795 L-ribulokinase GT1796 L-ribulose 5-phosphate 4-epimerase GT1797 D-Ribose Ribokinase GT3174 Transketolase GT1187 Ribose 5-phosphate epimerase GT3316 D-Xylose Xylose kinase GT1756 Xylose isomerase GT1757 D-Galactose Galactokinase GT2086 Galactose-1-phosphate uridyltransferase GT2084 UDP-glucose 4-epimerase GT2085 Carbohydrates* Enzymes Gene ID D-Fructose 1-phosphofructokinase GT1727 Fructose-1,6-bisphosphate aldolase GT1805 Fructose-1,6-bisphosphate aldolase type II GT3331 Triosephosphate isomerase GT3005 D-Mannose Mannnose-6 phospate isomelase GT3398 6-phospho-1-fructokinase GT2664 D-Mannitol Mannitol-1-phosphate dehydrogenase GT1844 N-Acetylglucosamine N-acetylglucosamine-6-phosphate deacetylase GT2205 N-acetylglucosamine-6-phosphate isomerase GT2204 D-Maltose Alpha-1,4-glucosidase GT0528, GT1643 Sucrose Sucrose phosphorylase GT3215 D-Trehalose Alpha-glucosidase GT1643 Glucose kinase GT2381 Inositol Myo-inositol catabolism protein iolC;5-dehydro-2- GT1807 deoxygluconokinase Myo-inositol 2-dehydrogenase GT1817 Myo-inositol catabolism protein iolE GT1809 IolD protein GT1810 Putative myo-inositol catabolism proteiniolB GT1808 Fructose-1,6-bisphosphate aldolase GT1805 Methylmalonate-semialdehyde dehydrogenase GT1806 Triosephosphate isomerase GT3005 Carbohydrates* Enzymes Gene ID Methyl-α-D-Glucopyranoside (MDG) Phospho- alpha -glucosidase GT0528, GT1643 Esculin ferric citrate Phospho-beta-glucosidase GT2266, GT3135, GT1747, GT3133 D-Cellobiose Beta -glucosidase GT1202 D-Melibiose Alpha -galactosidase GT2088 D-Melezitose Alpha-glucosidase GT0528, GT1643 Starch Alpha-amylase GT0614 Neopullulanase GT0610 Pullulanase type I GT2731 D-Turanose Phospho- alpha -glucosidase GT0528, GT1643

Putative protease and peptidase genes in G. thermodenitrificans NG80-2 genome Gene ID Gene product Class/family Specificity/comment GT1002 Lipoprotein signal peptidase A8, Signal peptidase (SPase) II GT0849 Acylaminoacyl-peptidase AXE1 GT2272, GT2288 Xaa-Pro aminopeptidase Creatinase_N GT2753 Putative peptidase DLH, Dienelactone hydrolase family GT1240 D-alanyl-D-alanine dipeptidase M15, D-ala-D-ala dipeptidase GT1125 Mitochondrial processing peptidase-like M16, Insulinase GT2899 Thermostable leucine aminopeptidase M17, Cytosol aminopeptidase family, catalytic domain Gene ID Gene product Class/family Specificity/comment GT1659, GT2291, GT2735 Putative peptidase T M20 GT0212, GT0214 Glycoprotein endopeptidase M22, Glycoprotease family Similar to O-sialoglycoprotein endopeptidase GT0127, GT1723, GT2342, Aminopeptidase M24, metallopeptidase family GT2676 GT2033 Aminopeptidase II M29, Thermophilic metalloprotease GT0702, GT0851, GT1704† Oligopeptidase F M3 Cleave medium sized peptides GT1393 Thermostable carboxypeptidase 1 M32, Carboxypeptidase Taq Thermostable carboxypeptidase GT2925 Cell wall endopeptidase, family M23/M37 M37 GT2637, GT2725 Deblocking aminopeptidase M42, glutamyl aminopeptidase GT0025 Signal peptidase II PSP1 GT1154 Renal dipeptidase family protein Renal_dipeptase GT0010, GT2214, GT2242† D-alanyl-D-alanine carboxypeptidase S11,D-alanyl-D-alanine carboxypeptidase GT0587, GT1056, GT1970 Type I signal peptidase S26, Signal peptidase I GT2694 Signal peptide peptidase S49 GT2482, GT2483 Peptidase, U32 family U32 GT1428† Carboxypeptidase VanY, D-alanyl-D-alanine carboxypeptidase GT2380 Endopeptidase I Zn_carbOpept, Zinc carboxypeptidase GT0221, GT2180 CAAX amino terminal protease family protein Abi, CAAX amino terminal protease family GT1299 Intracellular protease DJ-1_PfpI, DJ-1/PfpI family GT0210 Zinc metalloprotease DUF335, Putative metallopeptidase (SprT family) Gene ID Gene product Class/family Specificity/comment GT1141, GT1142 Zinc protease Peptidase_M16_C GT3353 Membrane metalloprotease Peptidase_M50 GT1721 Secreted protease metal-dependent protease Peptidase_M6, Immune inhibitor A peptidase GT3040 Putative protease Peptidase_S41 GT0188, GT1368, GT3101 Putative alkaline serine proteinase Peptidase_S8, Subtilase family GT2447 Spore protease Peptidase_U3, Germination protease GT0036 Sporulation-specific protease YabG Peptidase_U57, YabG peptidase GT0230 Membrane-bound protease, putative Transglut_core, Transglutaminase-like superfamily GT0286, GT2765, GT3410 Serine protease Trypsin GT0678, GT0856, GT1066, ATP-dependent protease ATPase family GT1067 GT2579, GT2580, GT2581 Lon protease S16

*Carbohydrates utilized by NG80-2 as tested by using API 50 CHB/E test strips (BioMérieux, Marcyl étoile, France) (Wang et al., 2006) (1).

†Genes having signal peptides but no transmembrane domains.

1. Wang L, Tang Y, Wang S, Liu RL, Liu MZ, Zhang Y, Liang FL, Feng L (2006) Extremophiles 10:347-356.