00 aE884 yacE 9268 b0103 ftsZ 4248 b0095 ftsA b0094 2737 imp b0054 4492 dnaJ b0015 00 uC3117 guaC b0104 1052 murG b0090 10437 leuC b0072 4770 leuD b0071 3825 surA 1588 b0053 folA b0048 7656 carB b0033 9312 carA 1331 b0032 lytB b0029 13283 dnaK b0014 20561 talB b0008 15039 thrC 6781 b0004 thrB b0003 00 hA13624 thrA b0002 B number
Gene name
mRNA intensity P P P P P P P P P P P P P P P P P P P mRNA present 19 48 14 16 13 32 20 18 39 13 0 22 18 17 18 10 42 7 9 70 # of tryptic peptides predicted P P P P P P P P P P P P P P P P P Protein detected - total list 17 23 13 10 18 4 8 2 4 3 6 1 9 9 5 1 3 # of tryptic peptides detected (total list) P(m) P(m) P(m) P(m) P(m) P(m) Proteins detected - membrane sample # of tryptic peptides M euts Metabolismofsmallmolecules Miscellaneous Metabolismofsmallmolecules GMPreductase Cellprocesses putativeDNA repairprotein Metabolismofsmallmolecules 0 0 3-isopropylmalate isomerase(dehydratase) subunit GenProt 2 Metabolismofsmallmolecules Metabolismofsmallmolecules isopropylmalate isomerasesubunit Metabolismofsmallmolecules 0 GenProt-PHD Metabolismofsmallmolecules 1 Cellprocesses Metabolismofsmallmolecules organicsolvent tolerance 0 dihydrofolate reductase typeI;trimethoprimresistance Metabolism of smallmolecules GenProt 0 carbamoyl-phosphate synthaselargesubunit Globalfunctions GenProt 5 carbamoyl-phosphate synthetase,glutamine(small)subunit 1 controlofstringent response;involvedinpenicillintolerance 0 chaperonewithDnaK;heatshockprotein 0 Metabolismofsmallmolecules 0 transaldolaseB 0 threoninesynthase 1 homoserinekinase 0 aspartokinaseI,homoserinedehydrogenaseI 0 0 0 2 detected (membrane sample)
Membrane protein assignment rti n TaeCellprocesses Cellprocesses protein andGTPase Cellprocesses cell division;forms circumferentialring;tubulin-like GTP-binding Structuralelements FtsZ, associated withjunctionsofinnerand outer membranes Cellprocesses ATP-binding cell divisionprotein,septationprocess, complexeswith pyrophosphoryl-undecaprenol N-acetylglucosaminetransferase UDP-N-acetylglucosamine:N-acetylmuramyl- (pentapeptide) outer membraneproteins(1st module) peptidyl-prolyl cis-transisomerase (PPIase),involvedinmaturationof proteins chaperone Hsp70;DNAbiosynthesis;autoregulatedheatshock
Gene description
Functional category 05 aR1779 yadR b0156 4087 acnB b0118 06 aD4162 dapD b0166 1127 yaeH 1016 b0163 1457 htrA b0161 pfs b0159 1105 fhuA b0150 5687 dksA b0145 5716 panB b0134 1978 panC b0133 952 yadG 2328 b0127 408 hpt b0125 gcd b0124 11091 speE b0121 21822 lpdA b0116 8150 aceF b0115 15349 aceE b0114 311 pdhR b0113 00 aC273 nadC b0109 B number
Gene name
mRNA intensity P P P P P P P P P P P P P P P P P P P mRNA present 60 15 16 32 41 17 34 0 44 15 13 17 38 80 8 80 7 8 4 50 6 # of tryptic peptides predicted P P P P P P P P P P P P P P Protein detected - total list 22 15 13 24 5 1 9 4 3 2 5 1 2 3 # of tryptic peptides detected (total list) P(m) P(m) P(m) P(m) P(m) Proteins detected - membrane sample # of tryptic peptides uaiesrcua rti Miscellaneous Metabolismofsmallmolecules Openreadingframes Macromoleculemetabolism Metabolism ofsmallmolecules Macromoleculemetabolism 2,3,4,5-tetrahydropyridine-2-carboxylate N-succinyltransferase Metabolismofsmallmolecules putativestructural protein periplasmicserine proteaseDo;heatshock proteinHtrA 0 Metabolismofsmallmolecules orf, hypothetical protein orf,hypothetical protein Metabolismofsmallmolecules 0 PHD Metabolismofsmallmolecules Metabolismofsmallmolecules 1 dnaKsuppressor protein Cellprocesses 0 3-methyl-2-oxobutanoate hydroxymethyltransferase MP-GenProt 0 pantothenate synthetase 3 putativeATP-binding componentofatransportsystem 0 Metabolismofsmallmolecules hypoxanthine phosphoribosyltransferase 0 0 spermidinesynthase =putrescineaminopropyltransferase Metabolismofsmallmolecules 0 aconitatehydrase B Metabolismofsmallmolecules glucosedehydrogenase 0 GenProt-PHD Metabolism ofsmallmolecules 1 pyruvatedehydrogenase (dihydrolipoyltransacetylasecomponent) Metabolismofsmallmolecules 0 pyruvatedehydrogenase(decarboxylasecomponent) 0 transcriptionalregulatorforpyruvatedehydrogenasecomplex 4 quinolinatephosphoribosyltransferase 0 1 0 0 detected (membrane sample)
Membrane protein assignment hgsT,T,adpi0Structural elements Metabolism ofsmallmolecules phages T1,T5,andphi80 outer membraneproteinreceptor forferrichrome,colicinM,and cleavage complex oxodehydrogenase andpyruvatecomplexes;L-proteinofglycine
Gene description
Functional category 01 aD1429 yajD b0410 1126 proC 465 b0386 ddlA b0381 2065 yahK 794 b0325 proA b0243 1500 pepD b0237 3000 gmhA b0222 3358 ykfE b0220 1976 yaeD b0200 16241 yaeC b0197 1361 proS b0194 1969 accA b0185 7246 fabZ b0180 4380 3673 yaeT b0177 frr b0172 8142 9865 pyrH b0171 tsf b0170 22176 rpsB b0169 06 a 3407 map b0168 B number
Gene name
mRNA intensity P P P P P P P P P P P P P P P P P P P mRNA present 13 29 18 0 40 11 18 11 16 15 16 20 19 10 90 60 9 40 80 8 # of tryptic peptides predicted P P P P P P P P P P P P P P Protein detected - total list 11 12 13 10 7 6 2 1 5 4 8 4 3 1 # of tryptic peptides detected (total list) P(m) P(m) Proteins detected - membrane sample # of tryptic peptides 2Gnrtof yohtclpoenOpenreadingframes orf,hypotheticalprotein GenProt 12 r,hpteia rti Openreadingframes Metabolismofsmallmolecules Structuralelements Miscellaneous Metabolismofsmallmolecules Structural elements Openreadingframes Miscellaneous orf,hypothetical protein Miscellaneous Macromoleculemetabolism pyrroline-5-carboxylate reductase D-alanine-D-alanine ligaseA Macromoleculemetabolism putativeoxidoreductase 0 gamma-glutamylphosphate reductase 0 aminoacyl-histidine dipeptidase(peptidase D) 0 Metabolismofsmallmolecules phosphoheptose isomerase Macromoleculemetabolism 0 orf,hypothetical protein 0 putativephosphatase 0 Metabolismofsmallmolecules putativelipoprotein 0 prolinetRNA synthetase Macromoleculemetabolism 0 Structuralelements Macromolecule metabolism Metabolism ofsmallmolecules acetylCoAcarboxylase, carboxytransferasecomponent,alphasubunit 0 (3R)-hydroxymyristol acylcarrierproteindehydratase 7 0 ribosomereleasingfactor 0 uridylatekinase 0 proteinchainelongationfactorEF-Ts 30SribosomalsubunitproteinS2 0 methionineaminopeptidase 0 0 0 0 detected (membrane sample)
Membrane protein assignment
Gene description
Functional category 08 sA1930 ushA b0480 3719 lon b0439 2012 clpX b0438 07 d 7814 adk 970 b0474 htpG 3354 b0473 apt b0469 5723 acrA 98 b0463 glnK b0450 1170 ybaU b0441 4300 7545 clpP b0437 tig b0436 3525 yajG b0434 3822 512 yajQ b0426 thiJ b0424 2792 ispA b0421 1747 yajO b0419 3916 nusB 3781 b0416 ribH b0415 01 s 2270 tsx b0411 B number
Gene name
mRNA intensity A P P P P P P P P P P P P P P P P P P mRNA present 22 24 0 10 12 19 10 21 13 37 21 33 37 70 7 7 8 5 8 7 # of tryptic peptides predicted P P P P P P P P P P P P P P P P P Protein detected - total list 17 14 8 3 2 1 4 4 5 7 8 4 1 1 2 4 2 # of tryptic peptides detected (total list) P(m) P(m) P(m) P(m) Proteins detected - membrane sample # of tryptic peptides D-ua yrls 5-uloiae Metabolismofsmallmolecules Cellprocesses Metabolismofsmallmolecules Cellprocesses Metabolismofsmallmolecules UDP-sugarhydrolase (5'-nucleotidase) Openreadingframes chaperoneHsp90, heatshockproteinC62.5 adeninephosphoribosyltransferase 0 Openreadingframes Miscellaneous 0 nitrogenregulatory proteinP-II2 acridineefflux pump 0 Global functions putativeprotease maturationprotein GenProt 0 DNA-binding, ATP-dependentproteaseLa; heatshockK-protein 5 Cellprocesses 0 7 Macromoleculemetabolism triggerfactor; amolecularchaperoneinvolvedincelldivision Metabolismofsmallmolecules 0 Openreadingframes putativepolymerase/proteinase Metabolismofsmallmolecules 0 orf,hypothetical protein Metabolismofsmallmolecules 0 4-methyl-5(beta-hydroxyethyl)-thiazole monophosphatesynthesis 0 geranyltranstransferase(farnesyldiphosphatesynthase) 4 putativeNAD(P)H-dependent xylosereductase 0 transcriptiontermination;Lfactor 0 Cellprocesses riboflavinsynthase,betachain 0 0 nucleosidechannel;receptorofphageT6 andcolicinK 0 GenProt 0 1 detected (membrane sample)
Membrane protein assignment clrnfrs ciiyMetabolism ofsmallmolecules Macromoleculemetabolism Macromolecule metabolism acyltransferase activity adenylate kinase activity;pleiotropiceffects on glycerol-3-phosphate chaperone ATP-dependent specificitycomponent ofclpPserineprotease, shock proteinF21.5 ATP-dependent proteolyticsubunitofclpA-clpPserineprotease, heat
Gene description
Functional category 06 bZ3091 ybeZ b0660 1907 rlpA b0633 1709 ybdQ b0607 3105 ahpF b0606 38698 ahpC b0605 8209 ppiB b0525 08 u 4618 fur b0683 1254 glnS b0680 892 nagE b0679 736 asnB b0674 5452 gltI b0655 2227 dacA b0632 6086 cspE b0623 371 entA b0596 2638 nfnB b0578 47106 ompT b0565 3543 purE b0523 2692 ybbN b0492 09 bL442 ybbL b0490 B number
Gene name
mRNA intensity P P P P P P P P P P P P P P P P P P P mRNA present 10 24 11 11 17 0 12 12 31 17 30 18 14 10 22 5 6 9 7 50 8 # of tryptic peptides predicted P P P P P P P P P P P P P P P P Protein detected - total list 6 4 6 4 6 5 5 5 5 3 5 4 1 9 1 4 # of tryptic peptides detected (total list) P(m) P(m) P(m) P(m) P(m) Proteins detected - membrane sample # of tryptic peptides eaiergltrGlobalfunctions Macromolecule metabolism Metabolismofsmallmolecules Macromoleculemetabolism Miscellaneous Cellprocesses negativeregulator Openreadingframes glutaminetRNA synthetase Cellprocesses asparaginesynthetase B 0 putativeATP-binding proteininphoregulon PTS system, N-acetylglucosamine-specific enzymeIIABC 0 GenProt-PHD 5 0 aminorlipoprotein 0 coldshockprotein GenProt Cellprocesses 0 orf,hypothetical protein GenProt 3 Miscellaneous 5 Macromoleculemetabolism 1 Structuralelements 0 oxygen-insensitive NAD(P)Hnitroreductase 0 0 peptidyl-prolyl cis-transisomeraseB(rotamaseB) Cellprocesses 0 outermembraneprotein3b(a),proteaseVII MP-GenProt 0 putativethioredoxin-likeprotein 6 PHD putativeATP-bindingcomponentofatransportsystem 0 0 0 0 detected (membrane sample)
Membrane protein assignment rti Cellprocesses Cellprocesses Cellprocesses Cellprocesses Metabolismofsmall molecules protein glutamate/aspartate transport systemperiplasmicbindingtransport Metabolismof smallmolecules protein 5 D-alanyl-D-alanine carboxypeptidase, fractionA;penicillin-binding hydroperoxides alkyl hydroperoxidereductase,F52asubunit;detoxification of hydroperoxides alkyl hydroperoxidereductase,C22subunit;detoxification of biosynthesis 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase,enterochelin catalytic subunit phosphoribosylaminoimidazole carboxylase=AIRcarboxylase,
Gene description
Functional category 05 pA5169 gpmA b0755 13638 pal b0741 7268 sucD b0729 07 bC2605 ybhC b0772 999 modA b0763 1742 galE b0759 4960 galM b0756 5196 aroG b0754 2364 nadA b0750 6035 ybgF b0742 6246 tolB b0740 20532 sucB b0727 5467 sdhB b0724 18927 sdhA b0723 11527 gltA 3721 b0720 ybgI b0710 1043 pgm 386 b0688 ybfF b0686 08 lA6895 fldA b0684 B number
Gene name
mRNA intensity P P P P P P P P P P P P P P P P P P P mRNA present 15 15 15 15 29 17 24 13 70 12 17 15 13 13 17 7 9 7 90 8 # of tryptic peptides predicted P P P P P P P P P P P P P P P P P Protein detected - total list 11 12 12 13 5 5 5 6 1 4 2 6 5 5 4 2 3 # of tryptic peptides detected (total list) P(m) P(m) P(m) P(m) P(m) Proteins detected - membrane sample # of tryptic peptides 1sciaedhdoeae rnslu rti Metabolismofsmallmolecules Metabolismofsmallmolecules succinatedehydrogenase,ironsulfurprotein succinatedehydrogenase,flavoproteinsubunit 11 11 uaiepcietrs Miscellaneous Metabolismofsmallmolecules Metabolismofsmallmolecules Metabolismofsmallmolecules Cellprocesses Openreadingframes Metabolismofsmallmolecules putativepectinesterase molybdate-binding periplasmicprotein;permease UDP-galactose-4-epimerase galactose-1-epimerase (mutarotase) 4 Macromoleculemetabolism phosphoglyceromutase 1 0 Metabolismofsmallmolecules Metabolismofsmallmolecules 0 quinolinatesynthetase, Aprotein 0 Openreadingframes orf,hypothetical protein 0 Metabolismofsmallmolecules Metabolismofsmallmolecules 0 Openreadingframes peptidoglycan-associated lipoprotein 0 succinyl-CoA synthetase, alphasubunit GenProt 0 5 0 0 0 citratesynthase orf,hypothetical protein PHD phosphoglucomutase orf,hypotheticalprotein 2 flavodoxin1 0 0 0 0 detected (membrane sample)
Membrane protein assignment oiisExtrachromosomal Metabolismofsmallmolecules Metabolismofsmall molecules synthetase, phenylalaninerepressible) 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase(DAHP A colicins periplasmic proteininvolvedinthetonb-independentuptake ofgroup component) 2-oxoglutarate dehydrogenase(dihydrolipoyltranssuccinase E2
Gene description
Functional category 09 eS643 serS b0893 09 oA2667 lolA 1462 b0891 trxB b0888 514 b0878 b0878 2647 ybjP 4200 b0865 artP 4421 b0864 artI b0863 2781 potF b0854 1054 b0830 b0830 193 moeB b0826 2262 ybiS b0819 6175 ompX 22609 b0814 dps b0812 16120 glnH b0811 3281 glnQ b0809 366 b0805 b0805 3559 moaB b0782 1308 bioD b0778 07 iB3252 bioB b0775 B number
Gene name
mRNA intensity A P P P P P P P P P P P P P P P P P P mRNA present 21 12 12 0 14 29 0 16 22 0 12 12 21 0 12 14 6 6 9 7 7 50 90 # of tryptic peptides predicted P P P P P P P P P P P P P Protein detected - total list 3 1 6 5 9 3 4 2 2 2 4 2 4 # of tryptic peptides detected (total list) P(m) P(m) P(m) Proteins detected - membrane sample # of tryptic peptides hoeoi euts Metabolismofsmallmolecules Miscellaneous Miscellaneous Macromolecule metabolism serinetRNAsynthetase; alsochargesselenocystein tRNAwithserine Cellprocesses Openreadingframes thioredoxinreductase Metabolismofsmallmolecules Cellprocesses 0 Cellprocesses putativeenzyme 0 Cellprocesses ATP-bindingcomponent of3rdargininetransport system putativemembrane protein MP-GenProt 0 Structuralelements arginine3rdtransport systemperiplasmicbinding protein Globalfunctions 0 periplasmicputrescine-binding protein;permease protein 4 putativetransport protein 0 Metabolismofsmallmolecules Cellprocesses 0 molybdopterinbiosynthesis orf,hypothetical protein Metabolismofsmallmolecules 0 PHD Metabolismofsmallmolecules 0 globalregulator, starvationconditions 0 periplasmicglutamine-binding protein;permease outermembraneproteinX Cellprocesses MP-GenProt 0 Miscellaneous ATP-bindingcomponentofglutaminehigh-affinitytransportsystem 2 0 molybdopterinbiosynthesis,proteinB 0 dethiobiotinsynthetase putativeoutermembranereceptorforirontransport MP-GenProt 2 biotinsynthesis,sulfurinsertion? 0 0 0 0 detected (membrane sample)
Membrane protein assignment ebaet ue Cellprocesses membrane toouter periplasmic protein effectstranslocationoflipoproteins frominner
Gene description
Functional category 11 uA358 putA b1014 14 dG3791 mdoG b1048 1237 ycdO b1018 6589 wrbA 2197 b1004 agp b1002 730 b0965 b0965 3808 mgsA b0963 36891 ompA b0957 794 ycbY b0948 1955 pyrD b0945 32813 ompF b0929 8281 aspC b0928 1614 ycbL b0927 751 msbA b0914 29325 rpsA 2547 b0911 cmk b0910 5838 serC 5044 b0907 pflB b0903 00 fA875 pflA b0902 B number
Gene name
mRNA intensity P P P P P P P P P P P P P P P P P P P mRNA present 36 20 16 23 26 23 10 0 17 38 14 10 25 21 57 15 16 8 9 8 60 # of tryptic peptides predicted P P P P P P P P P P P P P P P P Protein detected - total list 16 20 10 15 19 1 2 6 3 3 7 2 6 1 7 2 # of tryptic peptides detected (total list) P(m) P(m) P(m) P(m) P(m) P(m) P(m) Proteins detected - membrane sample # of tryptic peptides 9M-ePo ue ebaepoen3 I*Gd Structuralelements outermembraneprotein3a(II*;G;d) MP-GenProt 19 r,hpteia rti Openreadingframes Cellprocesses Openreadingframes Metabolismofsmallmolecules Metabolismofsmallmolecules Metabolismofsmallmolecules Openreadingframes periplasmicglucans biosynthesisprotein orf,hypothetical protein prolinedehydrogenase, P5Cdehydrogenase Metabolismofsmallmolecules 0 Metabolism ofsmallmolecules Openreadingframes periplasmicglucose-1-phosphatase 0 orf,hypothetical protein 0 Metabolismofsmallmolecules methylglyoxal synthase Structuralelements 0 1 Structuralelements putativeoxidoreductase 0 dihydro-orotate dehydrogenase 0 Metabolism ofsmallmolecules Metabolismofsmallmolecules aspartateaminotransferase 0 orf,hypothetical protein outermembraneprotein1a (Ia;b;F) MP-GenProt 2 Metabolismofsmallmolecules Cellprocesses 6 30SribosomalsubunitproteinS1 0 cytidylatekinase ATP-bindingtransportprotein;multicopysuppressorofhtrB 0 GenProt-PHD 3-phosphoserineaminotransferase 3 formateacetyltransferase1 3 pyruvateformatelyaseactivatingenzyme1 0 0 1 0 detected (membrane sample)
Membrane protein assignment prtrMetabolismofsmallmolecules operator trp repressorbinding protein;affectsassociation oftrprepressorand
Gene description
Functional category 19 aD8101 fabD b1092 23085 yceD b1088 13 hP6704 phoP b1130 1171 potA b1126 5107 potD b1123 4100 ycfP b1108 3089 ycfO 598 b1107 ycfH 645 b1100 fabF b1095 42769 acpP b1094 24478 fabG b1093 5633 fabH b1091 1642 68 rne b1084 flgL 253 b1083 flgG 175 b1078 63 flgF b1077 flgE 216 b1076 flgD b1075 16 yC4514 pyrC b1062 B number
Gene name
mRNA intensity A A A A P P P P P P P P P P P P P P P mRNA present 14 0 17 10 11 13 43 16 90 11 19 14 0 11 14 15 6 90 20 3 80 90 # of tryptic peptides predicted P P P P P P P P P P P P Protein detected - total list 3 4 2 4 5 5 8 2 3 1 2 3 # of tryptic peptides detected (total list) P(m) Proteins detected - membrane sample # of tryptic peptides rncitoa euaoypoenGlobalfunctions Openreadingframes Openreadingframes Openreadingframes Metabolismofsmallmolecules Cellprocesses Cellprocesses transcriptional regulatoryprotein ATP-bindingcomponent ofspermidine/putrescine transport Metabolismofsmallmolecules spermidine/putrescine periplasmictransport protein Metabolismofsmallmolecules Openreadingframes orf,hypothetical protein 0 orf,hypothetical protein 0 Metabolismofsmallmolecules orf,hypothetical protein 0 3-oxoacyl-[acyl-carrier-protein] synthaseII 0 acylcarrierprotein 0 3-oxoacyl-[acyl-carrier-protein] reductase 0 Metabolismofsmallmolecules malonyl-CoA-[acyl-carrier-protein] transacylase 0 Structuralelements Structuralelements 0 orf,hypothetical protein 0 Structuralelements PHD 0 flagellarbiosynthesis; hook-filamentjunctionprotein Structuralelements Structuralelements 0 flagellarbiosynthesis,cell-distalportionofbasal-bodyrod 0 flagellarbiosynthesis, cell-proximalportionofbasal-bodyrod 3MP flagellarbiosynthesis,hookprotein 0 flagellarbiosynthesis,initiationofhookassembly 0 dihydro-orotase 0 0 0 0 detected (membrane sample)
Membrane protein assignment rnayaeMetabolismofsmall molecules Macromoleculemetabolism transacylase 3-oxoacyl-[acyl-carrier-protein] synthaseIII;acetylCoAACP RNA RNase E,membraneattachment,mRNAturnover,maturation 5S
Gene description
Functional category 15 cI2853 yciI b1251 7098 oppA b1243 2855 kdsA b1215 5617 minD b1175 15 cD341 yciD b1256 1533 oppD b1246 4865 adhE 34541 b1241 hns b1237 1036 galU 295 b1236 narH b1225 3419 hemM b1209 2638 prsA b1207 1146 ychF b1203 3006 dhaK2 b1199 618 ycgC 1352 b1198 treA b1197 1825 dadA b1189 2930 minE b1174 13 uB2271 purB b1131 B number
Gene name
mRNA intensity P P P P P P P P P P P P P P P P P P P mRNA present 15 16 12 19 10 21 27 20 14 22 00 20 26 41 0 13 25 6 80 2 7 # of tryptic peptides predicted P P P P P P P P P P P P P P P P Protein detected - total list 18 24 4 2 4 6 2 7 3 2 5 7 4 2 1 4 # of tryptic peptides detected (total list) P(m) P(m) Proteins detected - membrane sample # of tryptic peptides r,hpteia rti Openreadingframes Miscellaneous Cellprocesses Metabolismofsmallmolecules orf,hypothetical protein Metabolismofsmallmolecules putative outer membrane protein MP-GenProt oligopeptidetransport; periplasmicbinding protein 0 Miscellaneous Structuralelements 0 Macromolecule metabolism DNA-binding protein HLP-II(HU,BH2,HD, NS);pleiotropicregulator Metabolismofsmallmolecules 0 Cellprocesses glucose-1-phosphate uridylyltransferase Miscellaneous 0 1 Metabolismofsmallmolecules 2-dehydro-3-deoxyphosphooctulonate aldolase nitratereductase 1,betasubunit 0 GenProt Metabolismofsmallmolecules 0 Metabolismofsmallmolecules phosphoribosylpyrophosphate synthetase 0 putativeGTP-binding protein GenProt 0 putativedihydroxyacetone kinase(EC2.7.1.2) 2 putativePTS system enzymeI 0 trehalase,periplasmic 0 D-aminoacid dehydrogenase subunit 0 Cellprocesses 0 0 adenylosuccinatelyase celldivisioninhibitor,amembraneATPase,activates minC MP-GenProt 0 0 0 0 detected (membrane sample)
Membrane protein assignment rnpr ytmCellprocesses Metabolismofsmallmolecules transport system homolog ofSalmonella ATP-bindingprotein of oligopeptideABC Metabolism ofsmallmolecules dehydrogenase; pyruvate-formate-lyase deactivase CoA-linked acetaldehydedehydrogenase andiron-dependentalcohol Cellprocesses possibly glutamyl-tRNAdehydrogenase an enzymeinmainpathwayofsynthesis5-aminolevulinate, ftsZ ringformation cell divisiontopologicalspecificityfactor,reversesMinCinhibition of
Gene description
Functional category 13 dA38 ydaA b1333 7051 tpx b1324 2495 yciO b1267 14 14 598 b1440 648 b1440 tehB b1430 282 ydcG b1424 8892 aldA b1415 486 ydbC b1406 208 ydbH b1381 457 mppA b1329 662 tyrR 5468 b1323 fabI b1288 248 b1284 982 b1284 pyrF b1281 1147 sohB b1272 3403 trpD 7588 b1263 trpB 1889 b1261 trpA b1260 15 cF943 yciF b1258 B number
Gene name
mRNA intensity A P P P P P P P P P P P P P P P P P P mRNA present 60 16 70 0 27 11 15 10 18 15 18 14 22 12 0 29 0 23 12 41 27
7 8 8 # of tryptic peptides predicted P P P P P P P P P P P P P P Protein detected - total list 19 5 2 1 2 4 9 5 4 3 1 9 3 1 # of tryptic peptides detected (total list) P(m) Proteins detected - membrane sample # of tryptic peptides elrt eitneCellprocesses Cellprocesses Miscellaneous Openreadingframes Openreadingframes Metabolismofsmallmolecules Openreadingframes Cellprocesses putativetransport protein telluriteresistance putativeglycoprotein aldehydedehydrogenase, NAD-linked 0 putativedehydrogenase 0 Miscellaneous orf,hypothetical protein 0 Metabolismofsmallmolecules Openreadingframes orf,hypothetical protein Metabolismofsmallmolecules Miscellaneous 2 0 thiolperoxidase 0 0 Metabolismofsmallmolecules enoyl-[acyl-carrier-protein] reductase(NADH) Openreadingframes 0 Metabolismofsmallmolecules putativeDEOR-type transcriptionalregulator 0 orotidine-5'-phosphate decarboxylase 0 0 putativeprotease orf,hypotheticalprotein 0 PHD 0 tryptophansynthase,betaprotein 0 tryptophansynthase,alphaprotein 0 putativestructuralproteins 0 0 0 0 detected (membrane sample)
Membrane protein assignment rnpr Cellprocesses Cellprocesses of multipleantibioticresistance periplasmic mureintripeptide transport protein,alsonegativeregulator transport transcriptional regulationofaroF,aroG,tyrAandaromatic amino acid Metabolismofsmallmolecules phosphoribosylanthranilate transferase anthranilate synthasecomponentII,glutamineamidotransferase and
Gene description
Functional category 12 d 867 add b1623 15 oB12459 sodB b1656 6502 849 ydhD b1654 rnt b1652 429 nemA b1650 1595 sodC b1646 15928 slyB b1641 549 pdxY b1636 467 b1631 b1631 2814 hdhA b1619 3778 ydgA b1614 1185 b1604 b1604 3267 pntB b1602 11538 b1583 b1583 654 b1498 67 b1498 pqqL b1494 5905 osmC b1482 299 narW b1466 15 15 2465 b1452 b1452 B number
Gene name
mRNA intensity A P P P P P P P P P P P P P P P P P P mRNA present 80 48 10 11 12 26 19 0 13 22 0 14 15 00 18 0 10 12 6 6 8 5 90 4 90 # of tryptic peptides predicted P P P P P P P P P P P Protein detected - total list 10 1 4 6 6 1 1 6 2 3 2 # of tryptic peptides detected (total list) P(m) P(m) P(m) P(m) Proteins detected - membrane sample # of tryptic peptides ueoiedsuae rnCellprocesses Openreadingframes Macromoleculemetabolism Metabolismofsmallmolecules Cellprocesses Openreadingframes Metabolismofsmallmolecules superoxidedismutase, iron Metabolismofsmallmolecules Openreadingframes orf,hypothetical protein Openreadingframes RNaseT,degrades tRNA Openreadingframes N-ethylmaleimide reductase 0 superoxidedismutase precursor(Cu-Zn) 0 Openreadingframes Openreadingframes 0 pyridoxalkinase 2/pyridoxinekinase Miscellaneous 0 orf,hypothetical protein putativeouter membraneprotein MP-GenProt 0 adenosinedeaminase 2 Cellprocesses Miscellaneous 0 orf,hypothetical protein 0 orf,hypothetical protein Metabolismofsmallmolecules 0 0 orf,hypothetical protein 4 putativesulfatase pyridinenucleotidetranshydrogenase,betasubunit 0 GenProt-PHD putativepeptidase Metabolismofsmallmolecules 6 osmoticallyinducibleprotein 1 crypticnitratereductase2,deltasubunit,assemblyfunction 0 putativereceptor 0 0 0 0 detected (membrane sample)
Membrane protein assignment eyrxlto fbl cd Metabolism ofsmallmolecules dehydroxylation ofbileacids NAD-dependent 7alpha-hydroxysteroiddehydrogenase,
Gene description
Functional category 16 dA2812 ydjA b1765 2441 selD b1764 2794 gdhA b1761 612 b1757 b1757 1584 xthA b1749 2821 osmE b1739 4224 pfkB b1723 4088 pheS b1714 2419 btuE b1710 1279 ydiA 909 b1703 aroD 1795 b1693 ydiJ b1687 1745 ynhD 55069 b1682 lpp b1677 3969 pykF b1676 4312 b1667 b1667 925 b1664 628 b1664 ribE b1662 15 uR1323 purR b1658 B number
Gene name
mRNA intensity P P P P P P P P P P P P P P P P P P P mRNA present 20 10 12 0 15 47 14 27 14 11 18 00 15 23 20 16 16 18 3 5 9 4 # of tryptic peptides predicted P P P P P P P P P P P P P P P P Protein detected - total list 13 6 1 5 4 3 2 4 8 2 3 8 3 1 1 2 # of tryptic peptides detected (total list) P(m) P(m) P(m) Proteins detected - membrane sample # of tryptic peptides r,hpteia rti Openreadingframes Macromoleculemetabolism Globalfunctions Metabolismofsmallmolecules Metabolismofsmallmolecules Macromoleculemetabolism Cellprocesses orf,hypothetical protein selenophosphate synthase,H(2)Seadded to acrylyl-tRNA Metabolismofsmallmolecules Miscellaneous Openreadingframes NADP-specific glutamatedehydrogenase Macromoleculemetabolism putativethiosulfate sulfurtransferase Metabolismofsmallmolecules 0 exonuclease III 0 activatorofntrL gene Structuralelements 0 6-phosphofructokinase II;suppressorofpfkA 0 Miscellaneous Openreadingframes phenylalanine tRNAsynthetase,alpha-subunit 0 vitaminB12transport 2 Cellprocesses 0 orf,hypothetical protein 3-dehydroquinate dehydratase Metabolismofsmallmolecules Metabolismofsmallmolecules 0 PHD putativeoxidase 0 putativeATP-binding componentofatransportsystem 0 0 pyruvatekinaseI(formerlyF),fructosestimulated mureinlipoprotein 5 orf,hypothetical protein GenProt 0 possibleenzyme 3 Metabolismofsmallmolecules riboflavinsynthase,alphachain 0 transcriptionalrepressorforpurregulon,glyA,glnB,prsA,speA 0 0 0 0 detected (membrane sample)
Membrane protein assignment
Gene description
Functional category 18 a 196 tar b1886 1559 yebL b1857 18 hA148 cheA b1888 231 cheY b1882 1119 yecM b1875 997 yecO b1870 3190 yebC b1864 1831 eda b1850 238 b1834 b1834 4057 b1832 1360 b1832 prc b1830 30711 cspC b1823 5528 manX b1817 1064 yeaZ b1807 9156 mipA b1782 1358 yeaD b1780 41263 gapA b1779 1743 ansA b1767 16 pA667 sppA b1766 B number
Gene name
mRNA intensity A A A P P P P P P P P P P P P P P P P mRNA present 90 0 29 27 38 10 35 15 10 11 20 26 10 15 14 5 80 9 6 9 50 # of tryptic peptides predicted P P P P P P P P P P P P P P P Protein detected - total list 13 1 2 6 1 3 5 1 2 2 2 4 2 3 2 # of tryptic peptides detected (total list) P(m) P(m) P(m) P(m) P(m) P(m) Proteins detected - membrane sample # of tryptic peptides r,hpteia rti Openreadingframes Openreadingframes Openreadingframes Miscellaneous Openreadingframes Openreadingframes Miscellaneous Cellprocesses orf,hypothetical protein methyl-accepting chemotaxisprotein II, aspartate sensorreceptor 0 GenProt-PHD orf,hypothetical protein 0 Openreadingframes orf,hypothetical protein 0 Cellprocesses 0 putative adhesin Openreadingframes Macromoleculemetabolism 0 PHD orf,hypothetical protein 0 orf,hypothetical protein 0 Metabolismofsmallmolecules carboxy-terminal proteaseforpenicillin-bindingprotein3 0 coldshockprotein 1 Metabolismofsmallmolecules PTSenzyme IIAB, mannose-specific 0 Macromoleculemetabolism Structural elements orf,hypothetical protein 0 1 orf,hypothetical protein Scaffoldingproteinformurein-synthesising holoenzyme. 1 glyceraldehyde-3-phosphatedehydrogenaseA GenProt 0 cytoplasmicL-asparaginaseI 3 proteaseIV,asignalpeptidepeptidase 0 2 0 1 detected (membrane sample)
Membrane protein assignment hBadCe Cellprocesses Cellprocesses Metabolism ofsmallmolecules CheB and CheY sensory transducerkinase betweenchemo- signalreceptors and motor components chemotaxis regulator transmitschemoreceptor signalstoflagelllar hydroxyglutarate aldolase 2-keto-3-deoxygluconate 6-phosphate aldolaseand2-keto-4-
Gene description
Functional category 22 zB1679 wzzB 1384 b2027 hisI 3366 b2026 hisF b2025 3393 yedU b1967 113 fliH b1940 2060 yedD b1928 392 fliC b1923 22 iA4310 hisA b2024 3584 hisD b2020 5358 565 hisG b2019 flu b2000 222 fliM b1945 213 fliG b1939 103 fliD b1924 94 fliA 5516 b1922 fliY b1920 1906 yedO 235 b1919 araF b1901 18 oB83 motB b1889 B number
Gene name
mRNA intensity A A A A A P P P P P P P P P P P P P P mRNA present 20 0 12 0 15 0 19 15 0 19 70 17 0 22 0 18 13 19 14 15 13 12 14 39 10 9 4 # of tryptic peptides predicted P P P P P P P P P P P Protein detected - total list 6 3 1 2 1 5 2 3 3 2 1 # of tryptic peptides detected (total list) P(m) P(m) P(m) Proteins detected - membrane sample # of tryptic peptides T hshrbslrnfrs Metabolismofsmallmolecules Openreadingframes PHD Openreadingframes Structuralelements 1 Metabolism ofsmallmolecules L-histidinal:NAD+ oxidoreductase;L-histidinol:NAD+ oxidoreductase 0 ATPphosphoribosyltransferase Structuralelements 0 0 outermembrane fluffingprotein,similar toadhesin orf,hypothetical protein 0 MP 0 flagellarbiosynthesis; exportofflagellarproteins? 3 0 Structuralelements Cellprocesses orf,hypothetical protein 0 Cellprocesses 0 flagellarbiosynthesis; flagellin,filamentstructuralprotein Miscellaneous 0 2 putativeperiplasmic bindingtransportprotein 0 putative1-aminocyclopropane-1-carboxylatedeaminase 0 L-arabinose-bindingperiplasmicprotein 0 0 0 Cellprocesses enablesflagellarmotorrotation,linkingtorquemachinerytocellwall 0 GenProt-PHD 0 detected (membrane sample)
Membrane protein assignment hisStructuralelements Metabolism of smallmolecules Metabolismofsmallmolecules Metabolism ofsmallmolecules chains regulator oflengthO-antigen component oflipopolysaccharide pyrophosphatase phosphoribosyl-amp cyclohydrolase;phosphoribosyl-ATP Structuralelements HisH =imidazole glycerolphsphatesynthase holoenzyme imidazole glycerol phosphatesynthasesubunit inheterodimerwith imidazolecarboxamide isomerase N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4- Structuralelements Structuralelements Structuralelements enabling rotationanddetermining itsdirection flagellar biosynthesis,component ofmotorswitchandenergizing, enabling rotationanddeterminingitsdirection flagellar biosynthesis,componentofmotorswitchingandenergizing, assembly flagellar biosynthesis;filamentcappingprotein;enables flagellar operons flagellar biosynthesis;alternativesigmafactor28;regulation of
Gene description
Functional category 26 rB878 fruB b2169 728 bglX b2132 654 udk b2066 21 mC39955 746 ompC b2215 eco b2209 1276 rsuA b2183 1445 yeiP b2171 1209 cirA b2155 7569 mglB b2150 974 yehZ b2131 1194 metG 2110 b2114 mrp 949 b2113 thiD b2103 47588 gatY b2096 5460 galF 2839 b2042 rfbB 956 b2041 1393 rfbC b2038 glf b2036 22 n 4998 gnd b2029 B number
Gene name
mRNA intensity P P P P P P P P P P P P P P P P P P P mRNA present 15 34 13 0 13 13 13 13 19 20 24 18 0 14 0 30 20 37 9 8 9 9 # of tryptic peptides predicted P P P P P P P P P P P P P P P P Protein detected - total list 11 19 12 2 1 2 7 3 9 7 5 5 1 1 1 2 # of tryptic peptides detected (total list) P(m) P(m) P(m) Proteins detected - membrane sample # of tryptic peptides ctn eiepoes niio Macromoleculemetabolism Miscellaneous Structuralelements Macromoleculemetabolism Cellprocesses Miscellaneous ecotin,aserine proteaseinhibitor Macromoleculemetabolism 16Spseudouridylate 516synthase outer membrane protein 1b (Ib;c) MP-GenProt Metabolismofsmallmolecules putativeelongation factor 5 PTSsystem, fructose-specific IIA/fprcomponent 0 Metabolismofsmallmolecules Cellprocesses Metabolismofsmallmolecules 0 Metabolismofsmallmolecules galactose-binding transportprotein;receptor forgalactosetaxis 0 beta-D-glucoside glucohydrolase,periplasmic MP-GenProt 0 Metabolismofsmallmolecules 5 methioninetRNA synthetase 0 putativeATPase Metabolismofsmallmolecules 0 Metabolismofsmallmolecules phosphomethylpyrimidine kinase 0 tagatose-bisphosphate aldolase1 0 uridine/cytidine kinase 0 0 dTDP-glucose 4,6dehydratase 1 Metabolismofsmallmolecules dTDP-6-deoxy-D-glucose-3,5epimerase 0 UDP-galactopyranosemutase 0 gluconate-6-phosphatedehydrogenase,decarboxylating 0 0 0 0 detected (membrane sample)
Membrane protein assignment eurstn eepoutStructural elements Cellprocesses requires tonBgeneproduct outer membranereceptorfor iron-regulated colicinIreceptor;porin; Metabolism ofsmallmolecules glycine betainecholinetransport forosmoprotection) putative ABCsuperfamily(peri_bind) transportprotein(possibly a UDP-galtransferase homolog ofSalmonellaUTP--glucose-1-Puridyltransferase, probably
Gene description
Functional category 21 rT3551 argT b2310 1441 glpC b2243 22 aB7891 fabB b2323 792 pdxB 1262 b2320 usg b2319 2828 accD b2316 6475 hisJ b2309 2986 yfcE b2300 639 b2299 1290 b2299 pta b2297 5299 ackA b2296 8848 b2294 b2294 9713 nuoB b2287 15463 nuoE b2285 8178 nuoG 331 b2283 yfbB b2263 1392 menB b2262 3240 glpB b2242 23 lQ5247 glpQ b2239 B number
Gene name
mRNA intensity P P P P P P P P P P P P P P P P P P P mRNA present 31 15 10 10 0 40 0 10 27 14 18 13 21 12 16 15 12 7 70 50 70 # of tryptic peptides predicted P P P P P P P P P P P P P P Protein detected - total list 10 10 9 3 3 2 8 1 6 5 2 3 4 1 # of tryptic peptides detected (total list) P(m) P(m) P(m) Proteins detected - membrane sample # of tryptic peptides -xay-ay-are-rti]snhs Metabolismofsmallmolecules Metabolismofsmallmolecules Miscellaneous Openreadingframes Openreadingframes Metabolismofsmallmolecules Metabolismofsmallmolecules 3-oxoacyl-[acyl-carrier-protein] synthaseI erythronate-4-phosphate dehyrogenase Cellprocesses Openreadingframes putativePTS system enzymeIIAcomponent Metabolismofsmallmolecules 0 acetylCoA carboxylase, carboxytransferase component, beta subunit lysine-,arginine-, ornithine-bindingperiplasmic protein Metabolismofsmallmolecules 0 Miscellaneous PHD Metabolismofsmallmolecules 0 orf,hypothetical protein Metabolismofsmallmolecules 0 putativeregulator 0 phosphotransacetylase 0 Metabolismofsmallmolecules acetatekinase 0 orf,hypothetical protein 0 NADHdehydrogenase IchainB 0 NADHdehydrogenase IchainE 0 NADHdehydrogenase IchainG 0 putativeenzyme 1 dihydroxynaphtoic acidsynthetase Metabolismofsmallmolecules 0 5 Metabolismofsmall molecules sn-glycerol-3-phosphatedehydrogenase(anaerobic), K-smallsubunit 0 glycerophosphodiesterphosphodiesterase,periplasmic GenProt 0 MP-GenProt 0 0 1 detected (membrane sample)
Membrane protein assignment ytmCellprocesses system histidine-binding periplasmicprotein ofhigh-affinityhistidinetransport Metabolismof small molecules anchor subunit sn-glycerol-3-phosphate dehydrogenase(anaerobic),membrane
Gene description
Functional category 27 lB3434 nlpB b2477 29 p 6828 upp b2498 1327 b2494 5167 b2494 bcp b2480 8978 dapA b2478 6714 purC 7999 b2476 talA b2464 720 b2431 b2431 857 ucpA b2426 672 8031 pdxK b2418 crr 20122 b2417 ptsI b2416 26197 920 cysK b2414 lig 1430 b2411 gltX b2400 1039 evgA 652 b2369 fadL b2344 3474 yfcB b2330 22 rC2496 aroC b2329 B number
Gene name
mRNA intensity P P P P P P P P P P P P P P P P P P P mRNA present 00 17 10 0 28 13 0 32 23 11 13 15 13 70 10 17 10 11 17 13 21 15
6 # of tryptic peptides predicted P P P P P P P P P P P P P P P Protein detected - total list 15 1 8 7 2 2 1 4 5 2 4 6 6 1 5 # of tryptic peptides detected (total list) P(m) P(m) P(m) Proteins detected - membrane sample # of tryptic peptides r,hpteia rti Openreadingframes Metabolismofsmallmolecules Macromoleculemetabolism Metabolismofsmallmolecules Metabolismofsmallmolecules Cellprocesses Openreadingframes Miscellaneous uracilphosphoribosyltransferase orf,hypothetical protein thiolperoxidase, thioredoxindependent Macromoleculemetabolism dihydrodipicolinate synthase 0 Metabolismofsmallmolecules 0 lipoprotein-34 0 Cellprocesses transaldolase A GenProt 0 orf,hypothetical protein 4 Cellprocesses putativeoxidoreductase 0 Metabolismofsmallmolecules pyridoxal/pyridoxine/pyridoxamine kinase Metabolismofsmallmolecules 0 Macromoleculemetabolism PTSsystem, glucose-specific IIAcomponent 0 PEP-proteinphosphotransferase systemenzymeI Openreadingframes 0 cysteinesynthase A,O-acetylserinesulfhydrolaseA Miscellaneous 0 DNAligase 0 glutamatetRNAsynthetase,catalyticsubunit 0 putativepositive transcriptionregulator(sensorEvgS) Cellprocesses 2 0 putativeadenine-specificmethylase transportoflong-chainfattyacids;sensitivity tophageT2 0 chorismatesynthase GenProt 0 2 0 0 detected (membrane sample)
Membrane protein assignment ACRsnhts Metabolism ofsmallmolecules SAICAR synthetase phosphoribosylaminoimidazole-succinocarboxamide synthetase=
Gene description
Functional category 22 sU6702 iscU b2529 27 pE3926 rpoE b2573 1302 rseB 1797 b2571 rnc 882 b2567 glnB b2553 479 suhB b2533 1221 b2532 b2532 5460 iscA b2528 1758 yfhE b2527 3045 pepB b2523 2053 sseA 19329 b2521 ndk b2518 1419 gcpE b2515 3338 hisS b2514 2958 b2513 1897 b2513 yfgL b2512 5523 guaB b2508 25 lA10756 glyA b2551 3990 purM b2499 B number
Gene name
mRNA intensity A P P P P P P P P P P P P P P P P P P mRNA present 16 25 13 22 23 10 15 17 11 13 19 6 9 6 9 90 7 8 7 # of tryptic peptides predicted P P P P P P P P P P P P P P P P P P Protein detected - total list 16 14 10 6 6 6 9 1 5 5 1 3 3 1 5 1 2 5 # of tryptic peptides detected (total list) P(m) P(m) P(m) Proteins detected - membrane sample # of tryptic peptides Ns I,d N Macromoleculemetabolism Globalfunctions Metabolismofsmallmolecules Metabolismofsmallmolecules Global functions Openreadingframes RNApolymerase, sigma-Efactor;heatshock andoxidativestress Miscellaneous Cellprocesses regulatesactivity ofsigma-Efactor RNaseIII,ds RNA regulatoryprotein P-IIforglutaminesynthetase 0 serinehydroxymethyltransferase 0 Openreadingframes Metabolismofsmallmolecules 0 Miscellaneous putativeATP synthase betasubunit Macromoleculemetabolism 0 Macromoleculemetabolism Openreadingframes putativeFeS assembly protein 0 Metabolismofsmallmolecules 0 orf,hypothetical protein 0 aminopeptidase B,acyteinylglycinase(2ndmodule) 0 Macromoleculemetabolism 0 putativethiosulfate sulfurtransferase nucleosidediphosphate kinase 0 PHD orf,hypothetical protein 0 histidinetRNAsynthetase 0 orf,hypothetical protein 0 putativeserine/threonineproteinkinase Metabolismofsmallmolecules 0 IMPdehydrogenase 0 phosphoribosylaminoimidazolesynthetase=AIR 1 5 0 1 detected (membrane sample)
Membrane protein assignment earCellprocesses enhances synthesisofsigma32 inmutant;extragenicsuppressor,ma repair putative regulatorbelievedto be involvedinFe-Sproteinformationor ouaeRAeIIlta cinGlobalfunctions modulate RNAseIIIlethalaction
Gene description y
Functional category 27 n 11093 eno b2779 3443 ygaU b2665 29 gZ1971 ygfZ b2898 1808 dsbC 8676 b2893 868 prfB b2891 ptr 190 b2821 fucO b2799 247 sdaB b2797 7990 pyrG b2780 3341 recA b2699 6894 alaS b2697 3559 ygaG b2687 6568 proX b2679 2920 grpE b2614 37205 rimM b2608 3791 b2595 b2595 1606 clpB 641 b2592 trxC b2582 27 fD394 yfiD b2579 B number
Gene name
mRNA intensity P P P P P P P P P P P P P P P P P P P mRNA present 15 49 11 12 16 46 16 10 17 0 40 15 14 27 19
7 6 9 8 9 # of tryptic peptides predicted P P P P P P P P P P P P P P P P P P Protein detected - total list 13 14 15 6 7 3 2 6 1 4 1 1 7 3 2 1 1 4 # of tryptic peptides detected (total list) P(m) P(m) P(m) P(m) Proteins detected - membrane sample # of tryptic peptides r,hpteia rti Openreadingframes Macromoleculemetabolism Structuralelements Macromoleculemetabolism Metabolismofsmallmolecules Metabolismofsmallmolecules Metabolismofsmallmolecules orf,hypothetical protein Metabolismofsmallmolecules Macromoleculemetabolism proteindisulfide isomeraseII Miscellaneous peptidechain releasefactorRF-2 proteaseIII 0 Openreadingframes L-1,2-propanediol oxidoreductase 0 0 L-serine dehydratase (deaminase),L-SD2 CTPsynthetase 0 PHD Macromoleculemetabolism Macromoleculemetabolism enolase Macromoleculemetabolism 0 0 alanyl-tRNAsynthetase Cellprocesses 0 orf,hypothetical protein Metabolismofsmallmolecules 2 high-affinitytransport systemforglycinebetaineandproline 2 Metabolismofsmallmolecules orf,hypothetical protein 0 0 16SrRNAprocessingprotein 0 orf,hypothetical protein 3 heatshockprotein 0 putativethioredoxin-likeprotein 0 putativeformateacetyltransferase 2 0 0 0 detected (membrane sample)
Membrane protein assignment N-adAPdpnetcpoes Macromoleculemetabolism Extrachromosomal DNA- andATP-dependentcoprotease DNA strandexchangeandrenaturation, DNA-dependentATPase, protein repair phage lambdareplication;hostDNAsynthesis;heatshock protein;
Gene description
Functional category 34 iB1210 ribB b3041 1431 gshB b2947 2279 rpiA b2914 33 oC3776 tolC b3035 2675 255 ygiW b3024 sufI b3017 1421 yqhE b3012 1184 yqhD b3011 227 hybA b2996 1229 yggN b2958 164 ansB b2957 2563 yggS b2951 1164 yqgE b2948 2768 metK 1912 b2942 tktA 16984 b2935 pgk 5147 b2926 fba b2925 4032 yggB b2924 20 cT2003 gcvT b2905 B number
Gene name
mRNA intensity A P P P P P P P P P P P P P P P P P P mRNA present 50 15 00 12 10 15 20 20 18 12 0 11 13 22 20 0 14 0 17 20 12 7 9 6 # of tryptic peptides predicted P P P P P P P P P P P P P P Protein detected - total list 19 14 10 1 3 8 9 4 1 4 2 3 2 3 # of tryptic peptides detected (total list) P(m) P(m) P(m) Proteins detected - membrane sample # of tryptic peptides 0MP-GenProt 10 upesro tIMiscellaneous Openreadingframes Metabolismofsmallmolecules Miscellaneous Metabolismofsmallmolecules Metabolismofsmallmolecules Openreadingframes 3,4dihydroxy-2-butanone-4-phosphate synthase Openreadingframes Metabolismofsmallmolecules Openreadingframes orf,hypothetical protein Metabolismofsmallmolecules suppressorof ftsI 0 2,5-diketo-D-gluconate reductaseA putativeoxidoreductase Metabolismofsmallmolecules 0 hydrogenase-2 smallsubunit Metabolismofsmallmolecules 0 orf,hypothetical protein 0 periplasmicL-asparaginase II 0 orf,hypothetical protein 0 Metabolismofsmallmolecules orf,hypothetical protein 0 glutathionesynthetase 0 Metabolismofsmallmolecules 0 transketolase 1isozyme 0 Miscellaneous phosphoglyceratekinase 0 fructose-bisphosphate aldolase,classII 0 0 ComponentoftheMscSmechanosensitive channel ribosephosphateisomerase,constitutive 0 PHD 1 4 0 0 detected (membrane sample)
Membrane protein assignment fduhe hoooe Cell processes of daughterchromosomes outer membrane channel;specifictolerance tocolicinE1;segregation Metabolismofsmallmolecules Metabolism ofsmallmolecules propylamine donor,corepressorofmetgenes methionine adenosyltransferase1(AdoMetsynthetase);methyl and glycine cleavagesystem aminomethyltransferase (Tprotein;tetrahydrofolate-dependent) of
Gene description
Functional category 39 rC3960 yrbC b3192 1459 murA b3189 39 rF1343 yrbF b3195 328 dacB 925 b3182 greA 3175 b3181 hflB b3178 2106 mrsA b3176 2077 argG b3172 11021 nusA 5346 b3169 pnp b3164 508 yhbS 350 b3156 yraR b3152 1789 yraP b3150 1102 yraM b3147 11454 yqjD 827 b3098 ygjD b3064 749 b3052 b3052 222 b3051 b3051 34 34 4471 b3042 b3042 B number
Gene name
mRNA intensity A A P P P P P P P P P P P P P P P P P mRNA present 10 21 0 25 24 0 29 10 0 30 16 25 10 00 10 13 21 10 32 23 24
5 9 4 # of tryptic peptides predicted P P P P P P P P P P P P P P Protein detected - total list 7 8 3 3 1 1 1 4 8 4 6 2 2 2 # of tryptic peptides detected (total list) P(m) P(m) P(m) P(m) P(m) Proteins detected - membrane sample # of tryptic peptides r,hpteia rti Cellprocesses Cellprocesses Metabolismofsmallmolecules putativeATP-binding componentofatransport system Macromoleculemetabolism orf,hypothetical protein Openreadingframes Cellprocesses 0 Macromolecule metabolism transcriptionelongation factor:cleaves3'nucleotide ofpausedmRNA Miscellaneous Metabolism ofsmallmolecules 0 Miscellaneous 0 Openreadingframes similartophosphoglucomutases andphosphomannomutases Macromoleculemetabolism Metabolismofsmallmolecules 0 Macromolecule metabolism argininosuccinate synthetase degradessigma32, integralmembrane peptidase,celldivisionprotein MP-GenProt 0 transcriptionpausing; Lfactor 8 polynucleotide phosphorylase;cytidylatekinaseactivity 0 Openreadingframes Miscellaneous 0 orf,hypothetical protein orf,hypothetical protein 0 putativeperiplasmic protein GenProt 1 putativeglycosylase 0 orf,hypotheticalprotein 0 putativeO-sialoglycoprotein endopeptidase 2 putativekinase 3 1 orf,hypotheticalprotein 0 0 0 0 detected (membrane sample) MP-GenProt-
H uaiemmrn rti Miscellaneous putativemembraneprotein PHD Membrane protein assignment rti Structuralelements Structural elements carboxyvinyltransferase first stepinmurein biosynthesis;UDP-N-glucosamine 1- protein 4 D-alanyl-D-alanine carboxypeptidase,fraction B;penicillin-binding
Gene description
Functional category 37 rA1766 yrdA b3279 30 pO45038 rplO b3301 33843 rpoA 1413 b3295 fmt 895 b3288 yrdC b3282 4945 fis b3261 2703 accC b3256 10847 accB b3255 1362 yhdH b3253 3268 mreB b3251 1447 yhdE 806 b3248 tldD b3244 18040 mdh b3236 685 degQ b3234 4258 sspA b3229 1618 sspB b3228 1071 yhbL b3209 2705 yhbG b3201 30 hN2682 yhbN b3200 B number
Gene name
mRNA intensity P P P P P P P P P P P P P P P P P P P mRNA present 13 0 17 17 13 20 13 17 13 14 22 9 9 5 9 40 6 9 20 60 # of tryptic peptides predicted P P P P P P P P P P P P P P P Protein detected - total list 25 12 3 5 2 1 4 2 9 1 2 1 1 4 2 # of tryptic peptides detected (total list) P(m) Proteins detected - membrane sample # of tryptic peptides 0 iooa uui rti 1 Structuralelements Miscellaneous Openreadingframes Miscellaneous 50Sribosomal subunitproteinL15 Miscellaneous RNApolymerase, alphasubunit Macromolecule metabolism Openreadingframes 10-formyltetrahydrofolate:L-methionyl-tRNA(fMet) N-formyltransferase Metabolismofsmallmolecules orf,hypothetical protein 0 Metabolismofsmallmolecules Metabolismofsmallmolecules Macromoleculemetabolism 0 putative transferase Miscellaneous 0 PHD acetylCoAcarboxylase, biotincarboxylase subunit 0 acetylCoAcarboxylase, BCCPsubunit;carrier ofbiotin Cellprocesses 0 Globalfunctions putativedehydrogenase 0 regulatorofftsI, penicillinbindingprotein3,septationfunction 0 Openreadingframes orf,hypothetical protein 0 suppressesinhibitory activityofCsrA Globalfunctions 0 Miscellaneous malatedehydrogenase 2 serineendoprotease 0 regulatoroftranscription;stringentstarvationproteinA Cellprocesses 0 stringentstarvation proteinB 0 sigmacross-reactingprotein27A(SCRP-27A) 0 putativeATP-bindingcomponentofatransportsystem 0 orf,hypotheticalprotein 0 0 0 0 detected (membrane sample)
Membrane protein assignment rn ciao o rncito Macromoleculemetabolism trans activatorfortranscription site-specific DNAinversionstimulation factor;DNA-bindingprotein;a
Gene description
Functional category 31 hI4562 yhgI b3414 35 gB760 ugpB 529 b3453 gntR 7201 b3438 asd b3433 7847 glpD 149 b3426 rtcR b3422 1241 ompR b3405 7786 pckA 668 b3403 hslO 619 b3401 yrfE b3397 516 mrcA b3396 5176 aroK 2239 b3390 rpe 3910 b3386 gph 858 b3385 trpS b3384 5225 slyD b3349 1544 fkpA b3347 56613 fusA b3340 33 uA55718 tufA b3339 B number
Gene name
mRNA intensity A P P P P P P P P P P P P P P P P P P mRNA present 70 27 00 11 10 45 14 12 13 32 22 40 25 14 12 30 13 24 8 70 5 6 # of tryptic peptides predicted P P P P P P P P P P P P P P P Protein detected - total list 29 36 12 23 15 4 2 2 3 6 5 1 2 1 3 # of tryptic peptides detected (total list) P(m) P(m) P(m) Proteins detected - membrane sample # of tryptic peptides 2s-lcrl3popaedhdoeae(eoi)Metabolismofsmallmolecules sn-glycerol-3-phosphate dehydrogenase(aerobic) 12 euao fguoae(n)oeo Metabolismofsmallmolecules Metabolismofsmallmolecules Miscellaneous Cell processes sn-glycerol3-phosphate transportsystem; periplasmic bindingprotein Macromoleculemetabolism Openreadingframes regulatorofgluconate (gnt)operon Metabolismofsmallmolecules Metabolismofsmallmolecules Cellprocesses aspartate-semialdehyde dehydrogenase 0 Sigma54-dependent regulatorofrtcBAexpression 0 orf,hypothetical protein 0 Metabolismofsmallmolecules Metabolismofsmallmolecules Macromoleculemetabolism phosphoenolpyruvate carboxykinase 0 Hsp33.Redox regulatedchaperone 0 orf,hypothetical protein 0 Structuralelements 0 shikimatekinase I 0 D-ribulose-5-phosphate 3-epimerase peptidoglycansynthetase; penicillin-bindingprotein1A 0 GenProt-PHD Macromoleculemetabolism phosphoglycolate phosphatase Macromoleculemetabolism 2 Macromoleculemetabolism tryptophantRNAsynthetase 0 FKBP-typepeptidyl-prolyl cis-transisomerase(rotamase) Macromoleculemetabolism 0 FKBP-typepeptidyl-prolylcis-transisomerase(rotamase) 0 GTP-bindingproteinchainelongationfactorEF-G 0 proteinchainelongationfactorEF-Tu(duplicateoftufB) 0 0 0 3 detected (membrane sample)
Membrane protein assignment mF ue ebaepoensnhssGlobal functions ompF: outermembraneprotein synthesis response regulator(sensor,EnvZ) affectingtranscriptionofompCand
Gene description
Functional category 36 iJ26232 livJ b3460 30 lD782 lldD 913 b3605 mtlA b3599 1887 selA b3591 2239 glyS 1741 b3559 yiaF b3554 1432 yiaE b3553 13089 dppA b3544 571 kdgK 245 b3526 yhjG 332 b3524 yhiU b3513 21552 hdeA b3510 3775 1442 hdeB b3509 slp 2280 b3506 gor b3500 21127 uspA 43 b3495 nikA b3476 2715 b3472 b3472 35 iK5530 livK b3458 B number
Gene name
mRNA intensity A A A A A P P P P P P P P P P P P P P mRNA present 10 21 0 11 29 21 0 25 17 0 10 13 17 90 20 29 36 10 13 24
2 7 9 40 # of tryptic peptides predicted P P P P P P P P P P P P P Protein detected - total list 11 11 18 15 2 1 3 2 2 3 2 1 5 # of tryptic peptides detected (total list) P(m) P(m) P(m) P(m) P(m) P(m) Proteins detected - membrane sample # of tryptic peptides ePo -att eyrgns Metabolismofsmallmolecules Openreadingframes Miscellaneous Macromoleculemetabolism Cellprocesses Metabolismofsmallmolecules Openreadingframes Macromolecule metabolism Cellprocesses L-lactatedehydrogenase Openreadingframes selenocysteine synthase:L-seryl-tRNA(Ser) seleniumtransferase GenProt Openreadingframes glycinetRNA synthetase, betasubunit PTS system, mannitol-specificenzyme IIABC components 0 GenProt-PHD Miscellaneous orf,hypothetical protein 2 putativedehydrogenase 0 Metabolismofsmallmolecules dipeptidetransport protein 0 ketodeoxygluconokinase 2 Openreadingframes orf,hypothetical protein 0 Cellprocesses 2 orf,hypothetical protein 0 Cellprocesses orf,hypothetical protein putativemembraneprotein MP-GenProt 1 Structural elements 0 glutathioneoxidoreductase 0 universalstressprotein;broadregulatoryfunction? outermembraneproteininducedaftercarbonstarvation MP-GenProt 0 periplasmicbinding proteinfornickel 5 orf,hypotheticalprotein 0 Cellprocesses high-affinityaminoacidtransportsystem;periplasmicbindingprotein 0 0 0 1 0 detected (membrane sample)
Membrane protein assignment rti Cellprocesses protein high-affinity leucine-specifictransportsystem;periplasmicbinding
Gene description
Functional category 33 lU3616 glmU b3730 1216 kbl b3617 34 sA412 asnA b3744 28649 atpF b3736 36217 atpH b3735 16689 atpA b3734 11853 atpG b3733 5523 atpD b3732 4613 atpC b3731 2111 pstS b3728 1915 tnaA 983 b3708 gyrB 509 b3699 yidA b3697 1002 nlpA b3661 4820 rpoZ 1867 b3649 dut 3195 b3640 dfp 4177 b3639 rfaD b3619 31 iO1790 yibO b3612 B number
Gene name
mRNA intensity P P P P P P P P P P P P P P P P P P P mRNA present 20 22 22 40 12 11 0 17 18 15 19 13 10 25 17 23 19 5 4 7 30 # of tryptic peptides predicted P P P P P P P P P P P P P P P P Protein detected - total list 12 17 11 8 6 1 3 1 4 2 3 7 2 3 1 2 # of tryptic peptides detected (total list) P(m) P(m) P(m) P(m) P(m) P(m) Proteins detected - membrane sample # of tryptic peptides 7M-ePo ebaebudAPsnhs,F etr lh-uui Cellprocesses Cell processes membrane-boundATPsynthase, F1sector,alpha-subunit MP-GenProt 17 membrane-boundATPsynthase,F1sector, beta-subunit MP-GenProt 11 saaiesnhts Metabolismofsmallmolecules Metabolismofsmallmolecules asparaginesynthetase A Cellprocesses Metabolism ofsmallmolecules Openreadingframes membrane-bound ATPsynthase,F0 sector, subunitb MP-GenProt 0 Macromoleculemetabolism Metabolismofsmallmolecules membrane-bound ATPsynthase,F1 sector, delta-subunit Metabolismofsmallmolecules MP-GenProt 3 1 membrane-bound ATPsynthase, F1 sector,gamma-subunit Cellprocesses MP-GenProt Miscellaneous Metabolism ofsmallmolecules N-acetylglucosamine-1-phosphate uridyltransferase 7 membrane-boundATPsynthase, F1sector,epsilon-subunit MP-GenProt Macromolecule metabolism tryptophanase 0 DNAgyrasesubunit B,typeIItopoisomerase,ATPaseactivity 0 orf,hypothetical protein 0 Structuralelements 0 RNApolymerase, omegasubunit lipoprotein-28 0 deoxyuridinetriphosphatase GenProt 0 Macromolecule metabolism flavoproteinaffecting synthesisofDNAandpantothenatemetabolism Metabolism ofsmallmolecules 1 ADP-L-glycero-D-mannoheptose-6-epimerase 0 2-amino-3-ketobutyrateCoAligase(glycineacetyltransferase) 0 0 0 0 0 detected (membrane sample)
Membrane protein assignment hsht-idn rti Cell processes Miscellaneous phosphate-binding protein high-affinity phosphate-specific transportsystem;periplasmic mutase putative 2,3-bisphosphoglycerate-independentphosphoglycerate
Gene description
Functional category 37 lA3685 glnA b3870 30 oA20896 sodA b3908 2901 yihK b3871 2297 dsbA b3860 2496 pepQ b3847 2343 b3838 b3838 2156 ubiE 1535 b3833 udp 714 b3831 yigL b3826 3481 dapF b3809 2018 hemC b3805 2492 hemX b3803 964 wzzE 2414 b3785 rho 5763 b3783 trxA 1367 b3781 yifE 876 b3764 rbsK b3752 33 aA3868 tatA b3836 11090 rbsB b3751 B number
Gene name
mRNA intensity P P P P P P P P P P P P P P P P P P P mRNA present 00 10 0 10 17 21 16 21 0 13 13 20 20 10 20 12 80 8 8 8 80 6 # of tryptic peptides predicted P P P P P P P P P P P P P P Protein detected - total list 11 12 1 5 1 3 9 1 5 1 5 1 9 1 # of tryptic peptides detected (total list) P(m) P(m) P(m) P(m) P(m) Proteins detected - membrane sample # of tryptic peptides ltmn yteaeMetabolismofsmallmolecules Miscellaneous Cellprocesses Macromoleculemetabolism Miscellaneous Metabolismofsmallmolecules Openreadingframes superoxidedismutase, manganese Openreadingframes putativeGTP-binding factor glutaminesynthetase Metabolism ofsmallmolecules 0 prolinedipeptidase 0 orf,hypothetical protein Metabolismofsmallmolecules Metabolismofsmallmolecules 3 0 Openreadingframes Metabolismofsmallmolecules Component of translocase 0 Openreadingframes uridinephosphorylase GenProt 0 orf,hypothetical protein Metabolism ofsmallmolecules 1 diaminopimelate epimerase 0 porphobilinogen deaminase=hydroxymethylbilanesynthase Macromoleculemetabolism 0 uroporphyrinogen IIImethylase Cellprocesses 0 0 transcriptionterminationfactorRho;polaritysuppressor putativetransportprotein 0 thioredoxin1 GenProt 5 orf,hypotheticalprotein 1 ribokinase 1 D-riboseperiplasmicbindingprotein 0 0 0 0 detected (membrane sample)
Membrane protein assignment omt-eedn euto Macromolecule metabolism formate-dependent reduction protein disulfide isomeraseI,essentialforcytochrome csynthesisand 2-octaprenyl-6-methoxy-1,4-benzoquinone -->2-octaprenyl-3-methyl- ehx-,-ezqioeMetabolism ofsmallmolecules methoxy-1,4-benzoquinone
Gene description 6
Functional category 38 uG5933 nusG b3982 217 gldA b3945 40 uH3168 purH b4006 1053 purD b4005 1145 hemE 4076 b3997 thiE 4139 b3993 thiG b3991 8885 rpoB b3987 23694 rplL 34311 b3986 rplJ b3985 28105 rplA b3984 299 btuB 590 b3966 trmA 2997 b3965 ppc b3956 1558 hslU 1607 b3931 yiiU b3928 17449 glpK 7653 b3926 tpiA b3919 31 fA2928 pfkA b3916 B number
Gene name
mRNA intensity P P P P P P P P P P P P P P P P P P P mRNA present 12 0 10 31 0 19 47 13 26 28 10 18 40 28 17 14 13 63 4 9 5 8 # of tryptic peptides predicted P P P P P P P P P P P P P P P P Protein detected - total list 18 22 10 31 9 5 4 7 1 2 5 3 2 2 6 7 # of tryptic peptides detected (total list) P(m) P(m) P(m) P(m) Proteins detected - membrane sample # of tryptic peptides rprhrngndcroyaeMetabolismofsmallmolecules Metabolismofsmallmolecules Miscellaneous Metabolismofsmallmolecules Metabolismofsmallmolecules Structuralelements Structuralelements phosphoribosylglycinamide synthetase=GAR synthetase uroporphyrinogen decarboxylase thiaminbiosynthesis, thiazolemoiety 0 thiaminbiosynthesis, thiazolemoiety Macromoleculemetabolism 0 Macromoleculemetabolism RNApolymerase, betasubunit 0 Metabolismofsmallmolecules 50Sribosomal subunitproteinL7/L12 Metabolismofsmallmolecules 0 Openreadingframes 50Sribosomal subunitproteinL10 Metabolismofsmallmolecules 0 Structuralelements 50Sribosomal subunitproteinL1,regulatessynthesisofL1andL11 0 componentin transcriptionantitermination 1 Metabolism ofsmallmolecules Metabolismofsmallmolecules 0 tRNA(uracil-5-)-methyltransferase 3 phosphoenolpyruvate carboxylase MP-GenProt 0 4 glyceroldehydrogenase,(NAD) 0 PHD orf,hypothetical protein 0 glycerolkinase 0 triosephosphateisomerase 0 6-phosphofructokinaseI 0 3 0 0 detected (membrane sample)
Membrane protein assignment IA omlrnfrs;IPccoyrls Metabolismofsmallmolecules AICAR formyltransferase; IMPcyclohydrolase phosphoribosylaminoimidazolecarboxamideformyltransferase = Structural elements Cellprocesses bacteriophage BF23 outer membranereceptorfortransportofvitaminB12,Ecolicins, and chaperones heat shockproteinhslVU,ATPasesubunit,homologousto
Gene description
Functional category 44 f 6703 efp b4147 10097 mopA b4143 1416 malK b4035 47 fC2685 hflC 835 b4175 yjeR 1157 b4162 psd 1593 b4160 frdA 883 b4154 frdB b4153 35460 mopB b4142 1704 aspA 43 b4139 fumB b4122 2675 19 phnA b4108 yjcF 1196 b4066 tyrB b4054 1658 dnaB b4052 1002 lexA b4043 3422 lamB b4036 3061 malE b4034 41 cA30099 aceA b4015 B number
Gene name
mRNA intensity A A P P P P P P P P P P P P P P P P P mRNA present 20 0 32 17 20 0 17 28 0 10 17 17 24 20 16 12 32 26 40 8 9 4 6 # of tryptic peptides predicted P P P P P P P P P P P P P P Protein detected - total list 34 13 3 5 1 1 3 4 4 1 2 2 2 5 # of tryptic peptides detected (total list) P(m) P(m) P(m) P(m) P(m) P(m) P(m) Proteins detected - membrane sample # of tryptic peptides r,hpteia rti Openreadingframes Macromoleculemetabolism Macromoleculemetabolism Metabolismofsmallmolecules proteasespecific forphagelambdacIIrepressor orf,hypothetical protein Macromolecule metabolism Metabolismofsmallmolecules Openreadingframes Metabolismofsmallmolecules Openreadingframes fumaratereductase, anaerobic,flavoprotein subunit phosphatidylserine decarboxylase; phospholipid synthesis 4 fumaratereductase, anaerobic,iron-sulfur protein subunit GenProt 0 elongationfactor P(EF-P) 4 0 Metabolismofsmallmolecules Globalfunctions 2 Metabolismofsmallmolecules aspartateammonia-lyase (aspartase) Macromoleculemetabolism Metabolismofsmallmolecules 0 fumaraseB= fumarate hydrataseClassI;anaerobicisozyme 4 orf,hypothetical protein 1 orf,hypothetical protein 0 tyrosineaminotransferase, tyrosinerepressible 0 replicativeDNA helicase;partofprimosome Cellprocesses 0 regulatorforSOS(lexA)regulon 0 Cellprocesses 0 ATP-bindingcomponentoftransportsystemformaltose phagelambdareceptorprotein; maltosehigh-affinityreceptor 0 GenProt 0 isocitratelyase 1 0 0 7 detected (membrane sample)
Membrane protein assignment rti Cellprocesses Cellprocesses protein GroEL, chaperoneHsp60,peptide-dependent ATPase,heatshock suppressing itsATPaseactivity GroES, 10Kdchaperonebinds toHsp60inpres.Mg-ATP, Cell processes transport andchemotaxis periplasmic maltose-bindingprotein;substraterecognition for
Gene description
Functional category 42 aA224 iadA b4328 1019 ytfJ b4216 45 s 359 tsr b4355 211 fecA b4291 39294 pyrB b4245 36636 pyrI b4244 11796 yjgF b4243 1085 pmbA 2607 b4235 fbp 908 b4232 ytfQ 3081 b4227 ppa b4226 1447 msrA b4219 464 cysQ b4214 524 cpdB 3787 b4213 fklB 2574 b4207 rplI b4203 23491 rpsF 173 b4200 yjfH b4180 47 uA2778 purA b4177 B number
Gene name
mRNA intensity A A A P P P P P P P P P P P P P P P P mRNA present 90 0 29 31 13 11 10 31 10 10 21 90 19 19 25 14 7 70 7 9 7 9 # of tryptic peptides predicted P P P P P P P P P P P P P P P Protein detected - total list 15 14 2 4 1 2 4 5 2 3 2 2 8 2 1 # of tryptic peptides detected (total list) P(m) P(m) Proteins detected - membrane sample # of tryptic peptides sapry ietds Macromoleculemetabolism Openreadingframes Metabolismofsmallmolecules Metabolismofsmallmolecules Cell processes Metabolismofsmallmolecules isoaspartyldipeptidase methyl-accepting chemotaxis proteinI,serine sensorreceptor Metabolism ofsmallmolecules Macromoleculemetabolism GenProt Openreadingframes aspartatecarbamoyltransferase, catalyticsubunit Cellprocesses 0 aspartatecarbamoyltransferase, regulatory subunit MP-GenProt 0 Macromoleculemetabolism orf,hypothetical protein 0 maturationof antibiotic MccB17,seetldgenes 2 fructose-bisphosphatase 0 putativeLACI-type transcriptionalregulator Metabolismofsmallmolecules 0 Structuralelements Macromoleculemetabolism inorganicpyrophosphatase 0 Structuralelements peptidemethionine sulfoxidereductase 0 Metabolismofsmallmolecules orf,hypothetical protein 0 0 Macromolecule metabolism 2':3'-cyclic-nucleotide 2'-phosphodiesterase 0 FKBP-type22KDpeptidyl-prolylcis-transisomerase(rotamase) 0 50Sribosomal subunitproteinL9 0 30SribosomalsubunitproteinS6 0 orf,hypotheticalprotein 0 adenylosuccinatesynthetase 3 0 0 0 detected (membrane sample)
Membrane protein assignment ebaercpo Cellprocesses membrane receptor outer membrane receptor;citrate-dependent irontransport,outer Metabolismof smallmolecules sulfite synthesis affects poolof3'-phosphoadenosine-5'-phosphosulfateinpathway of
Gene description
Functional category 49 l 746 slt b4392 2940 deoB b4383 6115 osmY b4376 47 rC1117 prfC b4375 Membrane proteinassignment(seeCorbinet al.2003):MP-list#1,GenProt#2,PHD#3. Annotation informationaccordingtoBlattner etal(1997).FunctionalcategoriesareassignedasdefinedbyRileyandLabedan( One proteincouldnotbematchedtoaBnumber andthuswasremovedfromconsideration. Lopez-Campistrous etal.(2005)identified575 proteinsofE.colion2Dgelmaps.Thedatawasextractedfromsupplementarydat B number
Gene name
mRNA intensity
P P P P mRNA present 41 19 23
7 # of tryptic peptides predicted P P P P Protein detected - total list 4 7 3 3 # of tryptic peptides detected (total list) Proteins detected - membrane sample # of tryptic peptides hshpnouaeMetabolismofsmallmolecules Structuralelements Macromoleculemetabolism Cellprocesses solublelyticmurein transglycosylase phosphopentomutase hyperosmoticallyinducibleperiplasmicprotein peptidechainreleasefactorRF-3 0 0 0 0 detected (membrane sample)
Membrane protein assignment
Gene description 1996).
a pdffile. Functional category