Finance 1. Introduction Organogram Executive Summary Preface i Goal &Mandate Milestones, Vision,Mission, Contents 9 8 Publications 7 6 5 4 3 2 Commercialisation OfTechnologies Consultancy, Patents& Mainstreaming ofGenderIssues Empowerment ofWomenand Research Collaborations Honours /Awards Dairy Education Transfer ofTechnology Research Achievements Extension Council Research AdvisoryCommittee Academic Council Board ofManagement Organisational Setup Historical Perspective Information Centre(ATIC) Agricultural Technology Krishi VigyanKendra(KVK) Dairy Extension Eastern RegionalStation,Kalyani Southern RegionalStation,Bangalore Dairy Extension Dairy Economics,Statistics&Management Dairy Engineering Dairy Microbiology Dairy Technology Dairy Chemistry Animal Biochemistry Dairy CattleNutrition Dairy CattlePhysiology Livestock ProductionAndManagement Animal Biotechnology Dairy CattleBreeding

Inside FrontCover 125 80 78 77 75 65 63 58 57 47 43 40 34 29 24 17 11 96 94 90 85 82 vi 5 4 4 3 2 2 1 6

15 14 Personnel 13 12 11 10 17. 16

ete 157 ArtificialBreedingResearchCentre

Library Livestock ResearchCentre Research Divisions Main Station Distinguished Visitors Field DaysOrganised Workshops/ TrainingProgrammes/ Conferences/ Seminars/Symposia/ Human ResourceDevelopment Monitoring andEvaluation(PME) Research Prioritization, Information jktHkk"kk ,dd fgUnh lkjka'k Regional Stations Support Sections Dairy ExtensionDivision Management Division Dairy Economics,Statistics& Dairy EngineeringDivision Dairy MicrobiologyDivision Dairy TechnologyDivision Dairy ChemistryDivision Animal BiochemistryDivision Animal BiotechnologyCentre Dairy CattlePhysiologyDivision Dairy CattleNutritionDivision Livestock ProductionandManagement Dairy CattleBreedingDivision Personnel Milestones Institute Staff Eastern RegionalStation,Kalyani Southern RegionalStation,Bangalore Model DairyPlant Human HealthComplex Maintenance Section Communication Centre Computer Centre Experimental DairyPlant Forage Res.&Mgmt.Centre Location andTravel

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Inside BackCover 152 152 152 151 151 150 150 149 149 148 148 148 146 142 169 165 159 153 141 139 129 127 173 163 163 163 162 160 160 162

ndri annual report 2013-14 ndri annual report 2013-14

July 2014 © Allrightsreserved Layout DesignandWordProcessing through hisinspiringorationon“Youth forTransformingIndianAgriculture”atNDRI Dr. S.Ayyappan,Secretary,DARE and DirectorGeneral,ICARignitingyoungminds Secretarial Operations Hindi Translation Cover Design Photography Chief Editor Publisher Editor

Technical Officer,PME Mr. SunilSharma, Associate Prof.(Eng.),NDRI Dr. (Mrs.)MeenaMalik, Joint Director(Res.),NDRI Dr. G.R.Patil, Director, NDRI Dr. A.K.Srivastava, Communication Centre,NDRI Assistant, DirectorCell Mr. ChiranjeeAggarwal, Assistant, PME Mr. RamDhari, Mrs. KanchanChaudhary, Mr. RamShankarGautam,DeputyDir.(OL) Sr. Tech.Officer(OL) izkDdFku dk;ZØeksa esa dk;ZØeksa dk;ZØeksa ds }kjk vkSj izksRlkgu feyk gSA bu vuqlaèkku vuqlaèkku lqO;ofLFkr ,oa lqfuf'pr gekjs dks iz;klksa o"kZ djrk gSA laLFkku ,oa bldh dk;Ziz.kkyh dk lEiw.kZ ifjn`'; izLrqr Msjh izfrf"Br bl ds ns'k os fd gS x;k fd;k O;ofLFkr ls izdkj bl dks lwpukvksa gSA x;k fd;k lekfo"V dks xbZ lacafèkr dk;Zdykiksa ds {ks= esa egRoiw.kZ miyfCèk;ksa izlkj ,oa bu dk;ZØeksa dks lqn`<+ djus ds fy, izkjaHk dh vuqHko gks jgk gSA bl izfrosnu esa Msjh vuqlaèkku] f'k{k.k] izfrosnu vkids le{k izLrqr djrs gq, eq>s vikj g"kZ dk jk"Vªh; Msjh vuqlaèkku laLFkku] o"kZ M-lV- j"ª; sh ok csZ (,u-Mh-Mh-ch-)] cksMZ fodkl Msjh jk"Vªh; (Mh-,l-Vh-) foHkkx izkS|ksfxdh ,oa (Mh-ch-Vh-) foHkkx tSoizkS|ksfxdh tSls ,tsfUl;ksa QafMx jk"Vªh; vxz.kh lHkh yxHkx dks ifj;kstukvksa ds vfrfjDr jk"Vªh; Msjh vuqlaèkku laLFkku gSaA fo'o cSad ls foÙkh; lgk;rk izkIr jgha py ifj;kstuk,a vuqlaèkku la;qDr baMks&U;wthySaM rFkk baMks&vk;jySaM (Mh-,l-Vh-&Mh-,-,-Mh-)] lsok,a fofue; 'kS{kf.kd teZu tSls ifj;kstuk,a vuqlaèkku orZeku esa laLFkku esa bl le; rhu vUrjjk"Vªh; leUo; vuqlaèkku laLFkku ds bfrgkl esa vc rd lcls vfèkd gSA 132-35 djksM+ :i, QafMax rd igqap xbZ gS tks fd ls jk"Vªh; Msjh ifj;kstukvksa vuqlaèkku foÙkh; gSa] vfrfjDr lfEefyr ifj;kstuk,a vuqlaèkku izkIr lgk;rk 2013&14 ds nkSjku laLFkku ds vuqlaèkku ,oa fodkl vUr%laLFkkuh; rFkk vUr%laLFkkuh; 89 2013&14 dh okf"kZd 19 ,u-,-vkbZ-ih- cká foÙkh; cká 74

Important Micro-organisms (NBAIM), (NDDB), NationalBureau of Agriculturally (DST), National Dairy Development Board (DBT), Department of Science and Technology agencies i.e.Department ofBiotechnology funding fromalmostalltheleadingnational NDRI hasbeensuccessful in gettingresearch Besides world bank funded 19 NAIP projects, Zealand research collaborative projects. (DST-DAAD), Indo-IrelandandIndo-New viz. GermanAcademicExchangeServices International CollaborativeResearchProjects Institute hasthedistinctionofhavingthree ever inthehistoryofNDRI.Presently, touched Funding from extramural research projects and 74externallyfundedresearchprojects. research programmescomprising89in-house impetus through well-defined and structured development programmeofNDRIgotfurther During theyear2013-14,researchand its functioning. this premier dairy Institute of the country and so arranged that itprovides a holistic view of these programmes.The information hasbeen and relatedactivitiesinitiatedtostrengthen area of Dairy Research, Education, Extension significant achievements of the Institute in the the period 2013-14. The report chronicles the before youtheAnnualReportofNDRI It gives me immense pleasure to present ` 132.35crores,whichisthehighest Preface for

i ndri annual report 2013-14 ii ndri annual report 2013-14 e'khu foftu iz.kkyh fodflr dh xbZA fy,ds ewY;kadu xq.koRrk ds mRiknksa Msjh vkèkkfjr ijekiu jax x;kA fd;k fy, ds mRiknu xktlkd fujUrj la'kksèku dk (,l-,l-,p-bZ-) fofuek;d Å"ek ljQsl LØsIM LVst Fkzh esa] {ks= ds ;a=hdj.k Mh-oh-,l-mRiknu ds fy, ds izkS|ksfxdh MksbZ ekufdr feLVh dh x,A xbZAfd, fodflr is; Ogs fdf.or (izksck;ksfVd)izfrtSoh; ls lÙo ;O; tkSa rjy rFkk fodkl lfEefyr gSA Ogs fLde nwèk dk izfØ;kvksa vadqfjr dh djus rS;kj dks mRiknksa tSls pht+ cktjk vkVk pht+ LizsM] fefJr olk pkdysV LizsM rFkk ehBk e`nq xSj Msjh la?kVdksa dk iz;ksx fd,A fØ;kRed izlaLÏr lh-,y-,- ;qDr fØ;kRed eD[ku] fØ;kRed Msjh ,oa okyh Msjh&Qy&vukt vkfèkkfjr lfEefJr ^lewnh* ykbQ 'ksYQ LFkk;h rS;kj gsrq iz;ksx rqjUr yLlh] iqf"Vdj yM+us djD;wfeu okyh xq.kksa izksQkbysfDVd okyh ls jksx ,Ythfe;j ftuesa gqbZ miyfCèk;ka vuqlaèkku egRoiw.kZ dqN esa {ks= ds izlaLdj.k Msjh esa fd;k x;kA ;g cNM+k lkseksfVd dksf'kdkvksa dk iz;ksx bl cMM+s dks mRiUu fo;ksftr ls oh;Z djus nzfor fge iz'khfrr ds lkaM bl FkhA ,sls lkaM dk Dyksu Fkk ftldh e`R;q dbZ o"kZ iwoZ gks pqdh 2013 dks mRiUu gqvkA ;g dVM+k Js"B Fkk pwafd ;g ,d lkaM ua- ijhf{kr larfr ,d xbZA gks e`R;q mudh dkj.k ds gksus ls nks vkSj Dyksu Hkh iSnk gq, ysfdu le; ls iwoZ mRiUu laLFkku dh mPpre nqXèk mRiknd HkSal FkhA ^dju dhfrZ* jghA ;g^djudhfrZ*ukedHkSaldkDyksuFkhtksfd bldk Hkkj ^iwf.kZek* uked ,d DyksuM dk tUe gqvkA tUe ds le; jgk gSA lkekU; izlo }kjk fnukad Dyksfuax ds {ks= esa viuh fo'ks"krk LFkkfir djus esa lQy laLFkku ,d ds ckn ,d dbZ DyksUM cNM+s mRiUu djds foÙkh; ls lgk;rk izkIr djus (Mh-,-,p-Mh-) esa lQy jgk gSA foHkkx Ms;fjax ,oa i'kqikyu rFkk (Mh-,-bZ-)] Hkkjrh; fpfdRlk vuqlaèkku ifj"kn] (vkbZ-lh-,e-vkj-) foHkkx ÅtkZ ijek.kq (,e-,Q-ih-vkbZ-)] (,u-,Q-ch-,l-vkj-,-) [kk| izlaLdj.k] m|ksx ea=ky; Ïf"k ij ewy ,oa egRoiw.kZ vuqlaèkku ds fy, jk"Vªh; QaM jk"Vªh; Ïf"k mi;ksxh lw{etho C;wjks (,u-ch-,-vkbZ-,e) –– Preface –– 4393 ls ,d DyksUM+ dVMk fnukad 44 fd -xzk FkkA ;g dVM+h 12 ?kaVs rd thfor jgkA 06 flrEcj] 21 fnu rd thfor 10 vxLr] 2013 dks

adulteration inmilkand milk products, the Likewise, todetectandcheck thehazardof products basedoncolourmeasurement. developed forqualityevaluation ofdairy of gajarpak.Machinevisionsystemwas was modified for continuous production stage ScrapedSurface Heat Exchanger (SSHE) misti doi.Intheareaofmechanization,three also standardized for the DVS production of and liquidbarleymaltextract.Technology was whey-skim milk, germinated pearl millet flour fermented beverage was developed from dairy andnon-dairyingredients.Probiotic and sweetenedsoftcheeseusingfunctional cheese spread,mixedfatchocolatespread butter containingCLA, functional processed cereal based composite smoothie, functional disease, ready-to-use shelf-stable dairy-fruit- prophylactic attributes against Alzheimer’s of products like curcumin fortified development oftheprocesses for preparation significant researchachievementsinclude In the area of dairy processing, some of the for 12h. used for producing this calf, which survived the frozen-thawedsemenofthisbullwere many years back.Somatic cells isolated from since it was thecloneofabull,whichhaddied born onAugust10,2013.Thiscalfwasunique tested bull4393was cloned calfofaprogeny A died duetoprematurebirth. were bornbut of theInstitute. Two more clones of Karan Kirti distinction ofbeingthehighestmilkproducer of abuffalonamed‘KaranKirti,havingthe which survived for 21 days, was the clone parturition onSeptember6,2013.Thiscalf, weighing 44kgwasbornthroughnormal the other.‘Purnima’,aclonedbuffalocalf by producingmanyclonedcalvesoneafter in establishingitsfortetheareaofcloning The Institutehasbeencontinuouslysuccessful Animal HusbandryandDairying(DAHD). Medical Research (ICMR) and Departmentof of Atomic Energy (DAE), Indian Council of Processing Industries (MFPI), Department on Agriculture(NFBSRA),MinistryofFood National FundforBasicandStrategicResearch , having lassi, having mikfèk;ka] Lo.kZ ind] esfjV izek.k i= rFkk iqjLdkj rFkk i= izek.k esfjV ind] Lo.kZ mikfèk;ka] dks Nk=ksa rFkk fd;k izLrqr mn~cksèku nh{kkUr viuk us fnYyh ubZ ifj"kn] vuqlaèkku Ïf"k Hkkjrh; lfpo] 2014 dks vk;ksftr fd;k x;kA Jh vjfoUn vkj-dkS'ky] dk laLFkku izHkkx iqjLdkj* Hkh tkjh gSA rFkk 'kS{kf.kd miyfCèk;ksa ,oa uoizorZuksa ds fy, ^Js"B ,oa LukrdksÙkj f'k{k.k ds fy, ^Js"B f'k{kd iqjLdkj* iwoZLukrd esa {ks= ds f'k{k.k jgkA tkjh nsuk iqjLdkj (Fkhfll) izcaèk 'kksèk Js"B gsrq Nk=ksa ds MkDVjsV ,oa izfrLi¼kZ dh Hkkouk dks izksRlkfgr djus ds fy, ekLVj xzg.k dj jgs FksA mPp xq.koRrk ds vuqlaèkku dk;Z ,oa esa ikB~;Øeksa fofHkUu jgs tk fd, izLrqr }kjk fo'ofo|ky; ekU; laLFkku] fn, gSaA o"kZ lkSai lQyrkiwoZd ls fofèk;ksa nksuksa vkQ&ykbu rFkk Ïf"kfo'ofo|ky;ksa ds Msjh foKku dkystksa dks vku&ykbu jkT; rFkk gSa x, fd, fodflr bZ&ikB~;Øe ds izkS|ksfxdh) (Msjh ch-VSd gSA fd;k izkIr LFkku izFke djds fodflr bZ&ikB~;Øe fy, ds dk;ZØe dksaiksuSaV&1 ih- ,u-,-vkbZ- us laLFkku vuqlaèkku Msjh jk"Vªh; gSA jgk ,oa Ms;fjax esa fMIyksek o"kZ dsUnz ifjlj] jk-Ms-vuq-laLFkku] dY;k.kh ij i'kqikyu jk"Vªh; Msjh fMIyksek Hkh izkjaHk esa dj fn;k gSA izkS|ksfxdh iwohZ {ks=h; Msjh ij ifjlj caxykSj dsUnz] {ks=h; o"kZ us laLFkku gSA fo'ofo|ky; Ïf"k Js"B prqFkZ laLFkku vuqlaèkku laLFkkuksa] jk"Vªh; ekU; Js"B dsUnz ,d ds :i esa Hkkjr ds dks lHkh ,d ekU; esa fo'ofo|ky;ksa]{ks=ksa vxz.kh ds laLFkku vuqlaèkku rFkk fo'ofo|ky; vuqlaèkku Msjh jk"Vªh; fofèk Hkh fodflr dh xbZA Fkk;sklkbusV dh ek=kRed tkap ds fy, ,d jax vkèkkfjr cká esa nwèk xbZA dh fodflr fofèk fo'ys"k.kkRed ,d iqf"Vdj VksUM nwèk esa foVkfeu Mh foVkfeu esa nwèk VksUM iqf"Vdj rFkk xbZ dh fodflr fofèk ,d fy, ds tkWap dh rsy ouLifr esa ?kh dj iz;ksx dk vkj-ih-,p-ih-Vh-,y-lh- tkWap ds fy, fLVªi vkèkkfjr ijh{k.k fodflr fd, x,A oSKkfudksa us nwèk esa fefJr ;wfj;k ,oa rVLFkhdkjdksa dh ds laLFkku vuqlaèkku Msjh jk"Vªh; fy, ds igpku rFkk tkap dh feykoV esa mRiknksa nqXèk rFkk nwèk izdkj blh 2013& oka nh{kkUr lekjksg fnukad lekjksg nh{kkUr 12oka ds vUrxZr ch-VSd (Msjh izkS|ksfxdh) (Msjh ch-VSd vUrxZr ds 14 ds nkSjku jk"Vªh; Msjh vuqlaèkku UjkVh N= f'k{k.k Nk= vUrjjk"Vªh; 12 2014& 2 ds vkdyu ds fy, ds vkdyu ds 15 ls izkjaHk fd;k tk 2013& ls nf{k.k ls 14 Qjojh] 14 49

14 Twelfth Convocationwasorganizedon also continued. achievements and innovations in teaching and ‘BestDivisionAward’foracademic Teacher Award’forUGandPGTeaching motivation forqualityresearch work. ‘Best to encouragethespiritofcompetitionand wascontinued students andDoctoral Master’s Deemed University. Best Thesis Award for NDRI offered by different programmesbeing of 12Internationalstudentswasonrollsin mode. Duringtheyear2013-14,atotalno. universities, bothinonlineaswelloff-line Dairy ScienceCollegesofstateagricultural developed and successfully delivered to all (Dairy Technology)programmehavebeen e-courses ofB.Tech component-1. Forty-nine underNAIP program degree Technology) by developinge-coursesforB.Tech.(Dairy 2014-15 onwards.NDRIhastakenthelead campus NDRI, Kalyani is being initiated from at Eastern Animal HusbandryandDairying Bangalore from the year2013-14.Diploma in in DairyTechnologyatSouthernCampus, also started offering NationalDairyDiploma reports.TheInstitutehasper thepublished 4 InstitutionsofIndia. Also NDRIranked as universities, NationalInstitutions,Research been ranked 41 among all universities, Deemed of excellence in frontierareasofresearch has NDRI asaDeemedUniversityandcentre detection ofextraneousthiocyanatesinmilk. method wasdevelopedforthequalitative fortified tonedmilk.Alsoacolourbased developed forestimationofvitaminD RP-HPTLC andananalyticalprotocolwas for vegetable oil detection ingheeusing neutralizers inmilk.Amethodwasdeveloped tests for thedetection of added ureaand scientists at NDRI developed strip based bestowed upon Dr. S. Ayyappan, Secretary Sundaresan Memorial Oration Award was and Awardstothestudents. FifthDr.D. Degrees, GoldMedals,Merit Certificates Convocation Addressand gaveawaythe Research, NewDelhi delivered the Secretary, IndianCouncilofAgricultural th th topAgriculturalUniversityofIndiaas February,2014.Sh.ArvindR.Kaushal, –– Preface –– 2 in in

iii ndri annual report 2013-14 iv ndri annual report 2013-14 kØsds] h vks lh ekbØksLdksi] QyksjkslSaV buojfVM dsfcusV] lqj{kk (tSo lqfoèkk,a xbZ gSA bl iz;ksx'kkyk dh lqfoèkkvksa esa dksf'kdk laoèkZu dh LFkkfir iz;ksx'kkyk Fkhfj;ksthuksyksth vR;kèkqfud ,u-,Q-ch-,l-,Q-,-vkj-,- ifj;kstuk ds vUrxZr ,d i'kq rFkk fØ;kdyki iz.kkyh vuqJo.k iz.kkyh nksgu izkjaHk ,oa nqXèk ewY;kafdr dh xbZA daI;wVjhÏr iz.kkyh] fMLiSaflax daI;wVjhÏr iz.kkfy;ksa] vkokl vR;kèkqfud rhu gsrq dY;k.k i'kq izHkkoh ,oa vkjke ds xk;ksa Msjh ,oa fodkl (ch-ih-Mh-) ,dd l`ftr fd;k x;k gSA fu;kstu O;olk; uohu vUrxZr ds ,u-,-vkbZ-ih- gsrq ;qok O;kolkf;;ksa esa Ms;fjax esa m|ferk dks izksUur djus fodkl dk;Z vk;ksftr djus ds fy, dkQh mi;ksxh gksxhA ekud fodkl rFkk ekuo lalkèku fodkl@vuqlaèkku ,oa miyfCèk gSA ;g iz;ksx'kkyk nqXèk ,oa nqXèk mRiknksa ds fy, vR;kèkqfud jsQjy iz;ksx'kkyk* dk l`tu ,d vU; cM+h LFkkfir dh xbZ gSA ^nqXèk xq.koÙkk ,oa lqj{kk ds fy, ,d p;kip; QsuksVkbi rFkk Qhft;ks&thuksfed iz;ksx'kkyk fy, ds vuqlaèkku izeq[k ,oa vxz.kh esa i'kqfoKku x;kA dk;e j[kus okyh i'kqèku vuqlaèkku dsUnz LFkkfir fd;k iz.kkyh dh lqfoèkkvksa ls ;qDr tyok;qoh; ifjorZuksa dks vfHkys[ku O;ogkj rFkk fØ;kRed 'kjhj 'kSYVj] i'kq i;kZoj.k pSEcj] lkbdksehfVªd pSEcj] dLVe fMtkbuM o"kZ fnYyh dks fn;k x;k ubZ ifj"kn] vuqlaèkku fpfdRlk Hkkjrh; egkfuns'kd] nLrwj Lekjd O;k[;ku bl o"kZ ds ,u-,u- Mk- fy, x;kA Mk-oh-,l-dVksp] fn;k }kjk fnYyh ubZ eaMy] p;u 2014 dks Mk- ,l-ds- caèkksikè;k;] lnL;] Ïf"k oSKkfud Mk- ds-ds- vkb;~;k Lekjd O;k[;ku fnukad fd;k x;kA fnukad }kjk fnYyh ubZ ifj"kn] vuqlaèkku Ïf"k Hkkjrh; egkfuns'kd] ,oa Ms;j laiUu gqvk rFkk ;g O;k[;ku Mk- ,l-v;~;Iiu] lfpo] O;k[;ku Lekjd Mh-lqUnjslu Mk- ikapoka fd;kA iznku –– Preface –– Ïf"k vuqlaèkku ifj"kn ds ekxZn'kZu rFkk izksRlkgu ,oa izksRlkgu rFkk ekxZn'kZu ds ifj"kn Ïf"k vuqlaèkku Ïf"k Hkkjrh; egkfuns'kd Hkkjrh; i'kqfoKku) (miegkfuns'kd] ifj"kn ,oa vuqlaèkku Ms;j lfpo] ;g lqfoèkk,a ,oa vU; ewy vuqlaèkku lqfoèkk,a lfEefyr gSaaA s kSk ik DskeV lh-vks- DysjksehVj i'kq nksSjku ds 2013&14 2 u;cV vf) fgLVksyksth vkfn) buD;wcsVj Qjojh] 11 dks izLrqr dks 2014 28 tuojh] 2

Generals and Principal Scientists at SMD support andhardworkofAssistant Director Director General (AS), ICAR, and also DARE &DirectorGeneralICAR andDeputy guidance andencouragement ofSecretary All thiscouldbeachievedwiththecontinued other basicresearchfacilities. histology facilities,proteomicsfacilitiesand for animalcalorimeter,CO Research Center’ has been set up with facilities During 2013-14,‘ClimateResilientLivestock Research, NewDelhi. Director General,IndianCouncilofMedical the yearwasbestowed uponDr.V.M.Katoch, Dr N.DasturMemorial Oration Award for of Agricultural Research, NewDelhi on 11 of Agricultural Indian Council DARE andDirectorGeneral, fluorescent microscope, CO microscope, fluorescent culture facilities(bio-safetycabinet,inverted NFBSFARA project.Thefacilitiesincludecell Laboratory has also been established under the cows. effective animalwelfareandcomfortindairy been commissionedandevaluatedfor animal activity monitoring system have and computerizedmilkingsystem system, computerized concentrate dispensing Three state-of-the-art animal housing with amongst youngprofessionals. to promote entrepreneurshipindairying (BPD)’ Unit hasbeenestablishedunderNAIP New ‘BusinessPlanningandDevelopment development formilkandproducts.A R&D/HRD developmentandstandards lab wouldbeofimmenseuseforconducting Laboratory forMilkQualityandSafety’.This the creationofstate-of-the-art‘ animal sciences. Another landmarkhasbeen has also been setupfor frontier research in phenotype andphysio-genomiclaboratory behavior monitoring system. Metabolic designed animal shelter, physiological and chambers, psychrometricchamber,custom Board, New Delhion28 Member, Agricultural Scientist Recruitment Award wasgiventoDr.S.K.Bandyopadhyay, MemorialOration 2014.Dr.K.K.Iya February, A state-of-the-artTheriogenology th January,2014and 2 2 nuao, etc.), incubator, environmental Referral th

ls tqM+s m|fe;ksa ds fy, vewY; lwpuk dk L=ksr gksxkA ds ns'k izfrosnu oSKkfud@'kS{kf.kd okf"kZd laLFkkuksa ,oa ;g vU; fd Msjh fodkl gw¡ laxBuksa djrk vk'kk eSa dh pjelhek rd igqap ldsA d:axk rkfd laLFkku vkxkeh o"kks± esa xkSjo ,oa lQyrkvksa ds fy, iw.kZ leiZ.k ,oa mÙkjnkf;Ro dh Hkkouk ls dk;Z fd muds lg;ksx ls eSa laLFkku ds y{;ksa dks izkIr djus gS fo'okl iw.kZ ugha gh vk'kk eq>s d:aA ppkZ ;gka dh vfèkdkfj;ksa ,oa laLFkku ds dkfeZdksa ,oa Nk=ksa ds ;ksxnku iz'kklfud@foÙk ladk;] izHkkxkè;{kksa] funs'kdksa] la;qDr tqM+s ls dk;Z bl eSa fd gS lkSHkkX; ije esjk ;g gSA ik;k gks laHko ls ifjJe dfBu ,oa lg;ksx ds lnL;ksa oSKkfudksa ,oa jk-Ms-vuq-laLFkku ifjokj ds lHkh lEekfur lgk- egkfuns'kdksa rFkk ,l-,e-Mh- (i'kqfoKku) ds izèkku (A. K.Srivastava) Organizations inthecountry. Institutions andotherDairyDevelopment Scientific/Academic the of professionals the serve asasourceofvaluableinformationto I sincerelyhopethatthisAnnualReportwould the yearstocome. and bringsuccess and glorytotheInstitutein fulfilling the mandate and objectives of NDRI efforts, wewould be abletomarchahead in support and with theirunrelenting sure that NDRI inthegrowthof theInstitute. Iam of students and Staff and Officers Finance Administrative/ Heads ofDivisions,Faculty, contributions madebytheJointDirectors, to acknowledge and place on record the the NDRIfraternity.HereIfeelprivileged (AS) aswellalltheesteemedmembersof –– Preface ––

v ndri annual report 2013-14 vi ndri annual report 2013-14 EXCUTIV SUMMARY and 550skilledsupportingstaff. 157 scientists, 260 technicians,145 administrative (Finance). The Institute presentlyhas strength of is undertheadministrativecontrol ofComptroller division finance whereas Registrar, and (Admn.) the administrativecontrol of theJointDirector viz. purchase,storesandsecurityareunder functions The administrative Section. Engineering Computer Centre,EstateSectionandMaintenance Unit, Libraryand National Bio-informatic Centre, Incubator, ExperimentalDairyPlant,Consultancy Complex, ModelDairyPlant,TechnologyBusiness AnimalHealth Centre, Research andManagement of centralfacilitiessuchasLivestockFarm,Forage consisting has infrastructure The Institute Centre. Training CentreandAnimalBreedingResearch Kendra andDairy Centre (ATIC),KrishiVigyan also has a Agricultural Technology Information stations. TheInstitute station anditstworegional Economics, StatisticsandManagementatthemain Dairy Microbiology,Extensionand Technology, Dairy Engineering, DairyChemistry, Biochemistry, Animal Biotechnology, Dairy Research, DairyCattlePhysiology,Animal Management, DairyCattleNutrition, Forage Cattle Breeding,LivestockProductionand Research Divisions/Sections,namely,Dairy and D activities aremanaged through thirteen iii) DairyExtension/Management.AlltheR i) DairyProduction,ii)Processingand has threemajorareasofRandDactivitiesviz. academic andextensionfunctions.TheInstitute by theJointDirectors formanaging research, assisted Officer Executive Chief the is Director Executive CouncilandExtensionCouncil.The Research AdvisoryCommittee, AcademicCouncil, decision makingbodiesviz.BoardofManagement, the Instituteismanagedthroughvariouspolicy/ of theDeemedUniversitySystemICAR, In consonance with the administrative pattern ORGANISATIONAL STRUCTURE programmes. University for implementing its educational Institute hasthedistinctionofbeingaDeemed suited to their agro-climatic conditions. The support region-specific providing for Kalyani at regional stations,oneatBangaloreandtheother present locationatKarnalin1955.Ithastwo headquarters oftheInstituteweremovedto country. Establishedin1923atBangalore,the towards dairydevelopmentprogrammesinthe Human Resource Development (HRD) support providing Research and Development (R&D) and research organization ofthenationdedicated for National DairyResearch Institute is the premier ● RESEARCH techniques intheirdissertation. have helpedstudentstooptmoderntoolsand digitization ofinformation.ThegrantsfromNAIP project monitoring, evaluation,computing and The fundswerealsoreceivedforstrengthening animals, nutraceuticalsandfunctionalfoods. biosensor, nanotechnology, abioticstress in farm cell, buffalo cloning, transcriptone, embryogenesis, agencies hasencouragedscientiststoworkonstem The supportfromNAIPandotherfunding one consultancyprojectamounting`1.85crores. worth mentioning that theInstitute is handling Animal HusbandryandDairying(DAHD).Itis Medical Research (ICMR) and Department of of AtomicEnergy(DAE),IndianCouncil Food Processing Industries (MFPI), Department Research onAgriculture(NFBSRA),Ministryof (NBAIM), NationalFundforBasicandstrategic of Agriculturally Important Microorganisms Bureau Board(NDDB),National Development of ScienceandTechnology(DST),NationalDairy of Biotechnology(DBT),Department Department almost allleadingnationalfundingagenciesi.e. been successful in gettingexternalfundingfrom Lead Instituteorconsortiumpartner.NDRIhas in consortiummodewhereinNDRIiseitherthe through NAIP has funded several research projects Newzealand jointresearchprojects.WorldBank Services (DST-DAAD),Indo-IrelandandIndo- projects includingGermanAcademicExchange NDRI hasthreeinternationalresearchcollaborative highest everinthehistory ofNDRI. Presently, projects hastouched`132.35crores,whichisthe research, fundingfromextramuralresearch 2013. Inordertostrengthenbasicandstrategic research projects were in operation during the year of89in-houseand75external number A total RESEARCH of Deemed University/KVK and Regional Stations. include the financial outlays for the strengthening 2013-2014 was ` sanctioned forplanandnon-plantheyear year 2013-2014was actual expenditureforplanandnon-planthe of terms in Institute the of outlays financial The BUDGET OUTLAY ● normal parturition, has the distinctionof being weighing 55kg,whichwas bornthrough was born onMarch 3,2013.This male calf ‘Swarn’, a clone of an existing bull Mu-5926 14531.09 lakhs. These figures These lakhs. 14531.09 ` 14501.47lakhsand budget ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● identified. Three potentialbiomarkers oflactationwere with milkyieldandlactation persistency. lactation revealed potential pathways associated epithelial cells duringdifferent stagesof Protein signaturesidentifiedinmammary NLR andinterleukinexpression. of different specific PRR agonist to measure the cultured nuclear cellsfromIndiancattleandbuffalowere tonsil) wasstudied.Peripheralbloodmono in differentimmuneorgans(blood, spleen and The basalexpressionofdifferentNLRgenes function ofthetissueasimmuneorgan. expressed indifferent tissues, irrespective of Buffalo RLR genes werefound tobeubiquitously dsRNA. shown to have different affinity towards mammals. RIG1 and MDA5 receptors were organization similartothatfoundinother were foundtofollowapatternofdomain Functional RIG1andMDA5genesofbuffalo from adultgoatfibroblastcells. stem cellsweregenerated Induced pluripotent stem cells. Oocytes weregeneratedfromgoatembryonic transgenic cells. mammary epithelialcells forproduction of Human insulingenewasexpressed in buffalo embryonic stem cells for their over-expression. were developedfortransfectionintobuffalo Buffalo NANOG,LIFandFGF2geneconstructs (Bubalus bubalis arnee) and oocytes from domestic buffalo (Bubalususing donorcellsfromwildbuffalo Inter-species cloned blastocysts wereproduced producing thiscalf,whichsurvivedfor12h. frozen-thawed semen of thisbullwereused for many years back.Somaticcells isolatedfromthe since it was the clone of a bull, which had died born onAugust10,2013.Thiscalfwasunique A clonedcalfofaprogenytestedbull4393was survived for21days. Institute, wasbornonSeptember6,2013.It history ofthe milk producerinthe highest the ‘Purnima’, acloneof‘KaranKirti’,whichis isolated fromfreshsemen. produced thoughtheuseofsomaticcells without and withthepresence in vitrowithout ). ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● were genotypedas13G/13G homozygotes. respectively whileallSahiwal (111)animals 12G/13G genotypeswere 0.965and0.035, and Fries (114)cattle,frequencies of13G/13G and KaranFriescattle,respectively. InKaran gene spansover3.772kband 3.789kbinSahiwal (FEZL) like finger zinc embryonic Forebrain improvement inthefield. sold tovarious agencies/farmersforgenetic A totalof215doses of frozensemen were herds. 545 females) calves were borninthefarmers’ up toDecember, 2012 and 1167 (622males and obtained out of 3562 A.I under field conditions In KaranFriescows,47.04%conceptionratewas ranged between5.03and12.28%. 4348-6861 kg.TheEPD of selectedmale calves lactation yield of reserved KFmales ranged from for futurebreeding. The dam’s best 305days conformity for selection of young male calves yield, breed characteristics and physical basis ofEPDanddam’s best 305dayslactation A totalof18KFmalecalveswerereservedonthe kg withanaverageof5528kg. day milkyieldrangedbetween4158and8648 305 kg) andaverageEPA(4114kg).Thebest and 12.29% higher thantheherd average (4250 and average EPA as 4620kgwhich were13.90% with anaverage305-daymilkyieldas 4841 kg matings cowsfornominated aselite selected A totalof68KaranFriescows (29.56%) were those ofnativelactoferrinisolatedfrommilk. dynamics of rec-Lfwerefoundtobesimilar Lf) wasproducedinyeast.Theironbinding Recombinant buffaloandgoatlactoferrins(rec- produced inE.coli. and fibronectin binding protein (Fbp) were (Cbp) protein binding collagen Recombinant and itsbindingwaslocalizedonoocytes. Associated 3 (SPACA3) protein wasproduced Recombinant buffaloSpermAcrosome cell line. was produced and purified from a mammalian Recombinant buffalo leukemia inhibitory factor associated proteinsweresequenced. Eleven different isoforms of buffalopregnancy cells. mammary epithelial of buffalo differentiation functional in role a have may which identified Sixteen differentiallyexpressedproteinswere –– Executive Summary––

vii ndri annual report 2013-14 viii ndri annual report 2013-14 ● ● ● ● ● ● ● ● ● ● ● –– Executive Summary–– ● ● ● ● ● ● ● ● ● ● ● calving period among the elite cows under field andinter- yield peak yield, milk daily yield, as milking uptoninemonthsare popularlycalled Cows giving birthtoonecalfevery yearand fall Malnadandcoastalregions ofKarnataka. role in thefarmingsystems of theheavyrain mostly thrivesongrazingandplaysaunique The Malnad Gidda is uniquedwarf cattle which conditions. in Murrahbuffaloes under sub-tropical climatic traits fertility of performance better for 75 ≤ be traits inMurrahbuffaloes. THIshould fertility The thresholdTHIwasidentifiedas75for organizations. frozen semen to farmers and dairy development The NDRIcentre supplied 62,054doses of 47.60% higherthantheherdaverage. buffaloes increased to 3151.29kg, which was The averagelactationmilkyieldofeliteMurrah under NetworkProjectonBuffaloImprovement. Five Murrahbullswereselectedfortestmating future breeding. elite Murrah male calves were reserved for milk yield(range2747-4090kg.),atotaloften (EPD) anddam’s best 305daysorlesslactation On thebasisofExpectedPredicted Difference was foundtobetranslocationcarriers. calves born ET five of screening Cytogenetical mastitis ascomparedtoothergenotypes. RFLP ofCD14genewereless susceptible to AB genotypedMurrahbuffaloeswithHpy188I- were mostsusceptibletomastitis. susceptible andAACCHH genotyped animals genotype of lactoferrin gene were least combined AACCGG with Murrah buffaloes observed. incidence ofclinicalmastitisincattlewas and S100A8genotypes between association respectively in Karan Fries cattle. No significant Sahiwal cattlewhileitwas0.973,0.02and0.007 to be0.884,0.102and0.013,respectivelyin SNPlocuswasfound -2(A/G) at genotypes The overallfrequencyofGG,GAandAA in cattle. using HpyCH4VforSNPgenotyping PCR-RFLP assaywasdevelopedforS100A8 respectively. cattle, inSahiwalandKaranFries 2.080 kb (Calgranulin) genecomprises of 2.036kband Entire coding and 5’ flanking regions of S100A8 Varshagandhi. Theaveragelactationmilk ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● cattle thanKFcattle. of TP season indicating thebetteradaptability TP than KF(P< 0.01) especially during winter The magnitudeofexpression washigherin Tharparkar (TP)andKaran Fries(KF)heifers. during winter than summer season in both (MC1R and PMEL) expressed highly (P<0.01) Genes responsible for skin pigmentation in buffalo. to heatstressbytranscriptomeandproteome relation in proteins and genes of Identification breeds. cattle in lines cell fibroblast 90 genesinvitro Expression pattern ofHSFs-1, 2, 4HSP-70,and PCR technique. buffalo was evaluated employingquantitative of fibroblast dermal the on stress heat of Effect reproductive managementinterventions. Deoni cattle through better nutritional and to augment reproductive performance in days toAFCandCIthereisfurtherscope indigenous cattleandthereisaneedtoreduce Deoni isamediumsized,dualpurpose season, etc. genetic factorsincludinghousing,parity, of SCMdependsonvariousgeneticandnon losses tothedairyfarmers.Theoccurrence which ultimatelyaccountsforheavyeconomic productivity andincreasesthecostoftreatment into clinicalformofmastitis,itreducesthe farmers. Ifsubclinical mastitis istransformed dairy economic lossestothe causes high threat Sub clinicalmastitis(SCM),amajorandsilent kinetics. (CS) designedonthebasisoffermentation was observed withconcentrate supplements compared topelletfeeds.ImprovementinSNF about 15%(overallmeanincrease13to17%) productionimproved protein Microbial kinetics. (CS) designedonthebasisoffermentation supplements cellulose improvedonconcentrate Digestible nutrientintakeofcellwallsand 627 breedingbullsin21shedswereevaluated. Indiamaintaining Southern ofthe parts different located inNine commercialsemenstations to behigher(range48.98157.10µg/ml). content inMalnadGiddacattlemilkwasfound than 15calves in theirlifetime.The lactoferrin ofcowscalvedmore number respectively. Good liters, 3.42±0.39litersand14.91 0.93 months, condition was 522.33 ± 69.40 liters,2.17± 0.29 ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● TGFβ secretion the activityofsomeantioxidant enzymesand functional markersofhepatocytes, increased important in decrease significant in resulted when exposed to hyperthermic conditions, which Caprine hepatocytes exhibited to be under stress buffalo calves. performance oflowbodyweightMurrah biotechnological toolforincreasingproductive proved tobeaneconomicandnoninvasive supplementation of fermented yeast culture male andfemaleMurrahbuffalocalves growth andreproductiveperformancein Plasma neutrophils wasobservedatcalving. Significantly higher (P<0.01) apoptosis of blood mammary glandtobecomemilkneutrophils. significantly (P<0.01)aftermigrationtothe viability andPAofbloodneutrophilschanged TNF-alpha, IL-1andIL-8atcalving.Shape, observed in neutrophilicgenes:TLR-2, TLR-4, was expression lower 0.01) (P< Significantly phagocytic activityofbloodneutrophils. in vitrosignificantly(P<0.01)decreasedthe Excess supplementation of micronutrients optimum productivity. period tomaintain amount duringperipartum available fromgreenfodderisrequiredinextra sufficiently be to thought is which A Vitamin had anegativeeffectontheirproductivity. whereas,copper andproductivity, immunity vivo toperipartumdairyanimalsimprovedtheir Vitamin EandAZnwhensupplementedin thermoneutral conditions. was higheratallperiodsascomparedwith the magnitudeofexpressionduringsummer condition ascompared to 0dayofcalving.Also post partumduringsummerandthermoneutral cows decreased significantly on 15 days pre and The Caspase-3 mRNA expression in SW and KF transition period. during summer inbothSWand KFcows during condition. TherewasupregulationofP-53 summer ascomparedwiththermoneutral KF cows at bothpreand post calving during BAX/BCL-2 ratios were higher inSWand conditions inSahiwal(SW)calves. and cortisollevelsduringextremeclimatic intake andreducedthefreeradicalproduction improved the healthstatus,immunityandfeed Supplementation ofchromiumpropionate

IGF I was positively related with . Treatment of hepatocyte in vitro.Treatmentofhepatocyte ● ● ● ● ● ● ● ● ● ● ● ● ● ● nutrients fromlignocellulosic feeds. gain, dailymilkyieldaswell asutilizationof of ruminantsforimproving livebodyweight potential tobeused as feed additive inthediet the showed diet, fibre high on kept buffaloes the rumenliquorofpermanentlyfistulated of containing wheat straw and the bacterial culture diet fibrous high on fed cattle crossbred Fries phylotype waspredominantlypresentinKaran The resultsrevealedthatMethanobrevibacter of rumen methanogens diversity in cattle. clustered in three orders providing better insights Compared to16SrRNAgene,mcrAgeneOTUs in aconcentratemixture. decorticated cottonseedcakesolventextracted of 15partsbycottonseedcakeexpelleror by replacing 10 parts of soybean meal out female KaranFriescalvesmaybereduced The cost forperkgbody weightgainin milk. responsible for CLA accumulation in meat and altering indirectlytheregulatorymechanism via modulationoftheabsorbedfattyacids thus pathways metabolic rumen of modification in Butyrivibrio activities. nitrogen, NEFAandimprovingtheliverenzyme urea blood the decreasing in effect beneficial a and feed efficiency in crossbred calves and had (RPC) (27g/day)improved average dailygain Supplementation ofrumenprotectedcholine cows ascomparedtocontrolgroup. increased milk yield and immunity in crossbred (6g), lysine(60g)andcholineresultedin Supplementation ofrumenprotectedmethionine level intherationoftransitionbuffaloes. on supplementingchromiumat1.0and1.5ppm nutrient utilisation was significantly improved and production milk of efficiency overall The by immunofluoroscencetechnique. could belocalizedonspermatozoaqualitatively factors growth Receptors forIGF-1andNGF growth factorsweresupplementedinvitro. bulls. Spermfunctionremainedunchangedafter parameters and fertilitystatus of Murrah buffalo were positivelyrelatedwithsperm functional like growthfactor-Iand nerve growthfactor The levelofplasmagrowthfactorsinsulin hyperthermic conditionsreversedtheeffects. cultures with mannan oligosaccharide under R. flavefaciens strainFD-1,isolatedfrom fibrisolvens adminstration resulted –– Executive Summary––

ix ndri annual report 2013-14 x ndri annual report 2013-14 ● ● ● ● ● ● ● –– Executive Summary–– ● ● ● ● ● ● ● A2 “like” milk. Oral intubation of commercially A2 “like”milk.Oralintubation ofcommercially of micethroughTh2pathway ascompared to of β-casein modulated the gut immune response Feeding of A1 “like” variants (A1A1 and A1A2) Cathepsin K. and acidphosphatase resistant viz. Tartarate the expressionofosteoclastspecificgenes decreasingacid phosphatase)cellsandby the number ofTRAP (Tartarate resistant inhibitory effectonosteoclastcellsbydecreasing and Osteocalcin.The peptide Calso exhibited viz. Collagen α1 type I, Alkaline phosphatase regulation ofosteoblastdifferentiationmarkers differentiation ofosteoblastsasevidentbyup and proliferation the stimulated β-casein from A sevenaminoacidpeptide(peptideC)isolated by Enterococcusfaecalis Molecular basisofresistanceagainstpediocin life ofmilk&productswasdeveloped. cheese wheyforapplicationinextendingshelf containing potentpowderfromindustrial A processforpreparationofapediocin pathway duringaging. which conclusivelysuggestedaskewedTh2 of humoral immunitywas evident inaging mice response, chronic inflammation and aggravation disorders. Adeclineincellmediatedimmune owing to infectious diseases and inflammatory increased morbidityand mortality inelderly Immunosenescence foremost reasonfor is the respectively. type I, type II methanotrophs and methanogens, for FTHFS, name seq1,kundu,radhaandbala 1571273, 1571282,and1571285withclones database under theBankit number 1570827, were obtained and deposited in the GeneBank sequence nucleotide The PCR. using amplified (methanothrophs and methanogens) were ftfs genes (acetogens) and 16S rRNA genes The partialnuceleotidesequenceencoding and highRFIgroups. methane productionascompared to medium (RFI) had higherfeed efficiency and less Sahiwal calves with low residual feed intake production. source with overall decrease in methane ruminant rationascheapfermentablenitrogen % calcium nitrateon DM basis can beused inthe similarly rumen fermentationparametersadversely in methane reduction without affecting other bromochloromethane wasfoundtobeeffective On thebasisofinvitrostudy,4mMdose studies, it was observed that 3 was observedthat in vitrostudies,it waselucidated. ● ● ● ● ● ● ● ● ● ● ● ● ● ● preparations ofAloeveraand Gymnema sylvestre of herbalingredientpowders (commercial in mice.However,thedietary incorporation rhamnosus indicates thepotentialofprobiotic (especially L. well asherbalingredientstohighfatdiet rhamnosus/ L.plantarum)fermentedmilkas Dietary supplementationofprobiotic(L. comparable tocontrolgroup. milk is values ofgoat to othergroupsandthe higher forgoatandcamelmilkascompared fed withmilk(3%).Antioxidativecapacityis deposition inliverdecreases in allthegroups in faeces.Atherogenicindexandcholesterol species increasesthelevelofcholesterolexcreted enriched diet.Feedingofmilkfromdifferent level whengivenalongwithcholesterol the increaseinplasmacholesterol fat) inhibits Consumption of milk from different species (3% existing inlowconcentrationfortheiranalysis. ofanalytesapplications inconcentration laboratory. Thesematerialshaveprofound Magnetic nanomaterialwaspreparedin or blue. change ingoldnanoparticlesfromredtoviolet aptamers and recognition is checked by colour requires coatingofgoldnanoparticleswith by selectedaptamerswascreated.Themethod Proof of principle of recognition of aflatoxin M1 change incolourgoldnanoparticles. with aptamers and recognition ischecked by method requires coating of gold nanoparticles aptamers wereintherangeof7.0-156nM.The betacasomorphine7. Dissociationconstantof for identified were aptamers Fifteen created. from typeA1milkbyaptamershasbeen betacasomorphin-7, afragmentgenerated Proof ofprinciplerecognition Tharparker) inlimitednumberofsamples. in indigenous breeds of cattle (Sahiwaland Karan Swiss (KS) butA1 was found absent in IndiancrossbredcattleKaranFries(KF)and found were β-casein of alleles A2 and A1 Both of β-casein)thanA1A2milk. genotyped KF was three times higher (0.2 mg/g A1A1 of milk from BCM-7 of Release β-casein. A1A2) butnotfrom A2 “like” (A2A2) variants of BCM-7 wasreleasedfromA1“like”(A1A1and inducing gutinflammation. that thesepeptidesweredirectlyinvolvedin synthesized BCM5 and BCM7 further confirmed ) in resisting the progression of obesity ofobesity theprogression ) inresisting ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● proteolytic skim milk by fermentation using selected from identified were peptides Antimicrobial oil andbuffalobodyfatinghee. methods were assessed for detection of soybean Based on physico-chemical properties, different estimation ofvitamin D An analytical protocol was developed for the detection ingheeusingRP-HPTLC. A Method was developed for vegetable oil detection ofaddedureaandneutralizersinmilk. weredevelopedfor the tests based Strip indicators ofcelldamageduetoNanotoxicity. liver enzymaticbiomarkersareefficient Mesoporous Nanoparticles( more thanthreetimes toxic as compared to Multiwalled carbonnanotubes(WCNTs)were yolk containingextender. spermatozoa over the conventionally used egg cryopreservation ofbuffaloandKaranFries of 30daysatrefrigeratortemperatureforthe life shelf extended with level field the at form developed in the ready to use concentrated The new phyto-basedsoya milk extenderwas independent pathways. through thecaspasedependentand spermatozoacryopreservation ofbuffalo depicts theonsetofactiveapoptosisduring in fresh and cryopreserved spermatozoa clearly The comparativeappraisalofapoptoticfeatures water buffaloes(Bubalusbubalis). between postpartumnormalandendometritic population differentialbacterial identified Metagenomic analysisofuterinemicrobiota metabolic pathwaysbyheatstressinzebucattle. transcriptomic signature highlightsthealtered Analysis of peripheral blood leukocytes fat dietfedconditionsinmice. positive effectonintestinalfunctionalityinhigh containing fermentedmilkseemstohavea Dietary supplementationofprobioticL.casei additive effects. used in thestudy)wasnotobserved to have Buffalo α Buffalo β-CN (197-209), β-CN (199-209), β-CN (193-207). nanoemulsion optimized using milk proteins A processforpreparation ofcurcumin for theirantioxidantactivity. s -casein hydrolysateswereassessed strains α strains lactobacillus 2 in fortified toned milk. MSNs). Different

S1 -CN (24-34),

● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● disease. prophylactic attributes against Alzheimer’s based low calorie spread were developed having Products like curcumin fortified antioxidants. replace syntheticantioxidantBHAwithnatural to an intention with products wasundertaken by- andvegetable offruit extracts using The workonextensionofshelf-lifeghee for extensionofitsshelflife. recommendedforpaneer canbe coatings edible stored well and was of similar quality. Therefore, dried. Paneercoated withstarch and casein concentrate and tapioca starch coatings and Paneer wascoatedwithcasein,wheyprotein bacteriophages. Regional Stationandtestedforthepresenceof the ExperimentalDairyofSouthern and skimmilksamples werecollectedfrom wheyThe samplesfromcheesewhey,paneer and dahi. lassi with theabovecombination of the whey were optimumforproductionofmostdesirable Use of20%waterand13%sugar preparation. could bereplacedupto20%ofdahiforthelassi whey hydrolysed andlactose concentrated were usedforlassipreparation,whilefresh and fermentation ofmilk and wheymixture be replacedupto10%whenfermentedwhey The sensory evaluation revealed that milkcan sorbitol basedandxyiltolsyrups. moisture contentofthejalebipreparedfrom as well as pH. There was also little changein slightly lowinfat,proteinand lactose contents in sweetenersyrups,thepreparedkhoa-jalebiwas It was also noted that on 100% sugar replacement control. resistant starch addedcheese was inferior to The body andtextureofboththeinulin was signedonMarch19,2014. 12.36%.. TheMOUbetweenNDRIandRCDF only) asonetimelicensefeeplusservicetax@ exclusive basisforRs.6,00,000(Rupeessixlakh The technologyhasbeentransferredonnon- SANKUL, J.L.N Marg, Jaipur-302 017, Rajasthan. “SARAS at office its having society registered Co-operative DairyFederation(RCDF)Ltd.a detergent inmilk”was transferred toRajasthan New colourbasedtestforrapiddetectionof model system. and assessed for antioxidantactivityinanimal lassi andchhana –– Executive Summary––

xi ndri annual report 2013-14 xii ndri annual report 2013-14 ● ● ● ● ● ● ● ● ● ● ● –– Executive Summary–– ● ● ● ● ● ● ● ● ● ● ● approach. storage waspreparedusingbiotechnological thermophilus cultures were selected for the Eight strainsoffastacidifying Streptococcus functional dairyfoods. to beapromisingprobioticorganism forusein potential inanimal model studies, has proved high hypocholesterolemicandanti-oxidative breast-fed human infants,exhibitingquite Lacobacillus gasseri enhanced safetyandshelf-life. promising techniqueforprovidingfoods with controlled release and may, thus,provetobea fortheir provides newerway bacteriocins Micro-encapsulation of broad spectrum GLP-1 forinducingsatiety. a roleinexpressionofguthormonessuchas However, CD14andG-proteinsmightplay role in GLP-1 expression by L.rhamnosus GG. Furthermore, MyD88 was observed to have no in vitrocelllinemodel of endoenterocrinecells. probiotic lactobacillicould be observed using Gut hormone secretionby indigenous strains of Lactobacillus fermentumLf1. demonstrated usinganindigenousstrainof fedC57BL/6Jmicecouldbe diet fat in high in glucosetoleranceandinsulinsensitivity demonstrated in animalmodel. Improvement glucose homeostasisas maintaining disease by prevent diabetes–amultifacetedmetabolic Probiotic strains were found to be able to Improved textured millet flourandliquidbarleymaltextract. made from whey-skim milk, germinated pearl Probiotic fermentedbeveragewasdeveloped ingredients. cheese using functional dairy and non-dairy fat chocolatespreadandsweetenedsoft functional processedcheesespread,mixed A technology developed for manufacture of cow milk. and extension of shelf life of A process wasstandardized forthemanufacture bioassay. its functionalityevaluatedthroughanimal incorporating CLAandphytosterol Functional butterwasdevelopedby based compositesmoothie. ready-to-use shelf-stable dairy-fruit-cereal A technology was developed to manufacture Lg70strain, isolated from dahi prepared for extended Kheer mohanfrom ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● during khoamanufacturein threestageSSHE Energy conservation studies was carried out milk andpearlmiletweredetermined. zinc fortified nutrimix (weaning food) based on Moisture sorptioncharacteristics ofironand designed anddeveloped. production of rabriusing in-linesystem was Forcedconvective mechanism for accelerated of gajarpak. (SSHE) was modified for continuous production ScrapedSurfaceHeatExchangerThree stage colour measurement. quality evaluation of dairy products based on wasdevelopedforMachine visionsystem machine wasdoneforhandlingconcentratedmilk. Scale updesignofcontinuouskhoamaking residues inmilkatfarmlevelwasdeveloped. Spore-sensor technology (Enzyme) for antibiotic based detectionsystemsweredeveloped. micro wellbiochip and miniaturized substrate marker enzymes and their germinogenic monocytogenes on96micro-wellplateusing Spore based assay fordetection of L. microflora. against food borne pathogens and spoilage exhibited broadspectrumantimicrobialactivity cinnamaldehyde, eugenol and carvacrol Plant bioactivecomponents i.e. thymol,trans- treatment ofhypercholesterolemia. of pathogen infections, immunomodulation and prevention usedforthe RS-2canbe fermentum L. diversity. of dairyoriginandexhibiteddistinctgenetic showed betterprobioticattributesthanthose Lactobacillus strainsofhumanorigininvariably concern. European Commission which is a matter of was foundtobehigherthanrecommended by Commission, 2008. MICformostoftheisolates following theguidelinesgivenbyEuropean sensitivity profile against 50 different antibiotics characterized andscreened for theirantibiotic of 26commercialprobioticproductswere Twenty eight isolates recovered from a total culture wasstandardized. ofmistidoi production DVS forthe Technology tolerance potential. sugar of mistidoibasedontheir preparation ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● cattle ration. utilization of some newer feed resources in dairy Comparative nutritional evaluation and cattle atERS,NDRI,Kalyani,WestBengal. selection efficiency for lactation traits of crossbred Development ofgeneticmodelforimprovingthe produced embryos. Cattle embryonic stem cells from improving growthperformancesincalves. using treeleaves of north-eastern India for Dietary manipulationofrumenfermentation empowerment. effective wayofincomegenerationand Group approach(SHGs)wasfoundmost grassroot innovatorsindairying. highlighted toemphasisagreaterroleof Subsequently, attributesofinnovationswere the motivationalfactorswereidentified. Characteristics ofgrassrootinnovatorsand need toberedressedonpriority. constraints thatarrestdairydevelopmentand revealing thatdairyfarmersarefacingumpteen in theregionrangedfrom` animals (64-76%). The cost of milk production Majority oftherespondentspossessed2-5dairy dairy productionsystemsinthesouthernregion. region andtostudy theconstraintsofsmallholder studies ofsuccessfulsmalldairyfarmersthe areas ofsouthernregion,toanalyseselectedcase holder dairyproductionsystemsintheselected small of profile the study to objectives specific systems inthesouthernregionwastakenupwith The profile study on small holder dairy production milk inmechanizedandmanualsystem. physico-chemical characteristicsofconcentrated Comparative studieswascarriedouton Sh. ArvindR.Kaushal(IAS),Secretary, ICAR&AdditionalSecretary,DARE 17-23/litre. It was 17-23/litre.Itwas giving awaydegreestothepassing outgraduates in vitro

● ● ● EDUCATION ● ● ● ● ● ● ● other institutionsofferingtheundergraduate State AgriculturalUniversities(SAU’s)and the to delivery offline and online for reviewers finalized through peer reviewers and student’s component-1. Thesecourseswerereviewedand programme werecompletedunderNAIP E-courses forB.Tech(DairyTechnology)degree and DairyEconomics. Production andManagement,AnimalNutrition discipline ofAnimalBiotechnology,Livestock inthe ofMastersStudents work dissertation Regional Station,Kalyaniwereusedfor Bangalore. Likewise, facilities atEastern Economics attheSouthern RegionalStation, andDairy Technology Dairy Chemistry, dissertation work inAnimal Breeding, Dairy Chemistry and Dairy Technology, and Doctoral Dissertations in Masters programme in Dairy students were deputed to carry out their strengthened. Intheperiodunderreport, The teachingattheRegionalStationswas students. boys and 192 girlsand this included 15 foreign possessed a strength of 837 students having 645 camus, Bangalorefrom2013-14.Theinstitute Diploma inDairyTechnology atSouthern alsostarted Institute the (Dairy Technology), course indifferentdisciplinesandB.Tech. In addition to ongoingMaster’s and Doctoral region ofIndia. on the economyof rural households in eastern Impact analysisofwomen dairy cooperatives crossbred calves-acasestudyinERS. Sex chromosome chimerism observed in twin

–– Executive Summary–– xiii ndri annual report 2013-14 xiv ndri annual report 2013-14 –– Executive Summary–– ● ● ● EXTENSION ● ● ● ● ● ● ● On 28 for quality research work. for qualityresearchwork. encourage spiritofcompetitionandmotivation also giventoMaster’sandDoctoralstudents February, 2014.“BestThesisAwards”were week ofJanuary,2014tothesecond Academic Monthwascelebratedfromsecond selection of students for Best Thesis Awards, etc. Divisions followedbydeliberationspanelists, innovations inteachingbydifferentHeadsof & presentationofacademicachievementsand of academic events such as Memorial lectures prior toConvocationbyorganizingtheseries In year2013-14,NDRIorganizedAcademicMonth occasion. Commissioner, were Guestsof Honour on the and Dr.S.Honnappagol,AnimalHusbandry Council ofAgriculturalResearch,NewDelhi Director General(AnimalSciences),Indian the students.Dr.K.M.L.Pathak,Deputy Medals, MeritCertificatesandAwardsto Address and gave awaytheDegrees, Gold Research, NewDelhideliveredtheConvocation Secretary, IndianCouncilofAgricultural management practices, mastitis control, disease control,disease mastitis management practices, were alsomadeawareabout thereproductive developed duringthelast one year.Farmers adopted villagesfortransfer oftechnologies with thedairyfarmersandfarm womeninthe Fifty threeKisanSangoshties wereorganized ICAR, bothinonlineaswelloff-linemode Colleges ofstate agricultural universities of successfully delivered toallDairyScience Technology) programmeweredevelopedand Forty-nine e-coursesofB.Tech (Dairy Council ofAgriculturalResearch,NewDelhi. to Dr.S.Ayyappan, Director General,Indian Memorial OrationAwardwasheldandgiven also completed. and revision in view of the regular feedback was of these e-courses interms of constant updating program. Thesecond phase ofimplementation 14 Twelfth Convocation was organized on on 11 Council of Medical Research, New Delhi and Dr. V.M.Katoch,DirectorGeneral,Indian Oration Award fortheyearwas bestowed upon Board, New Delhi; Member, AgriculturalScientistRecruitment Oration wasgiventoDr.S.K.Bandyopadhyay, th February,2014.Sh.ArvindR.Kaushal, th th February,20145 January,2014,Dr.K.K.IyaMemorial Dr N.DasturMemorial th Dr.D.Sundaresan ● ● ● ● ● ● ● ● ● ● ● ● and general veterinary cases. and generalveterinarycases. diseases, infertilityandforpregnancy diagnosis of animalsweretreatedagainst thecontagious Budhakhera andGanjogarhi. Alargenumber viz., Nasirpur,Nasirpultila,vazidpur, Kulwaheri, villages nearby at Visits Exposure Campaigns, NDRI organized63InfertilityandVeterinaryaid of farmers. benefit the for NDRI ATIC at point sale seeds time NDRI and PAU Ludhiana jointly starteda seeds, FYM,Vermi-Compost(` sale ofbooks(`162379)andfromthe the centre generated revenue of literature, tollfree phone calls and e-mails. The sows, lectures,personaldiscussions, published persons ofdifferentstates,throughvideo 15790ATIC NDRIprovided8802servicesto revenue worthRs.47,15,292/-duringtheyear. its multifariousactivities,KVKgenerated Through country. entire the from beneficiaries programmes wereattendedby These training farm women,ruralyouthandentrepreneurs. from home science for the benefit of 6873 apart farmers, fisheries and keeping bee horticulture, production, crop as such agriculture of fields on differentaspectsofdairyingandallied programmes (bothoncampusandoffcampus) KVK andDTCorganized230training in theInstitute. extension achievementsandfacilitiesavailable about thedifferent research, teaching and the institute.Thegroups have beensensitized 104 colleges/Institutions/Universitiesvisited of of 6121visitors(students&Faculty) A total Insemination (AI)coverage. of VeterinaryservicesandwideArtificial Karnal district undertaken. Prompt delivery of blocks 6 in villages 12 in aid first veterinary and PD AI, farming, dairy scientific in youth ofunemployedvillage building Capacity organized benefiting64farmwomen. production ofvalueadded dairyproducts capacity buildingprogrammes on small scale Four on-campus women entrepreneurship and healthy atmosphereintheirrespectivefamily. more income from dairying and maintain 511 farmwomen.Farmwomencouldgenerate benefiting organized campaigns and training Thirty four off-campus women empowerment and roundtheyeargreenfodderproduction. products, management offodderscarcityperiod practices, preparation of value added milk preventive measures, clean milk production ` 6,45,064/- from 482685/-). First INFRASTRUCTURE ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● with facilitiesforanimalcalorimeter,CO Climate ResilientLivestockResearchCenter for FSSAI. development/standards developmentwork of immenseuseforconductingR&D/HRD Safety createdatNDRI.Thislabwouldbe Referral LaboratoryforMilkQualityand cabinet, inverted fluorescent microscope, CO microscope, fluorescent inverted cabinet, (bio-safety facilities includecellculture facilities at NDRIundertheNFBSFARAproject.The “Theriogenology Laboratory”wasestablished “Theriogenology” Laboratory:Astateoftheart (BPD) UnitwascreatedunderNAIP. New Business Planning and Development sciences. laboratory setupforfrontierresearchinanimal Metabolic phenotypeand physio-genomic physiological andbehaviormonitoringsystem. chamber, customdesignedanimalshelter, environmental chambers,psychrometric facilities andotherbasicresearchfacilities. incubator, etc),histologyfacilities,proteomics e-content foronlinedelivery. creation, modificationand uploadingthe equipments andinfrastructure fore-content the Division. Itwas equippedwithallnecessary E-content developmentlab was established in and walkincoldroom shrink-wrap packagingmachine,fruitpulper processing machine,spectrophotometer, vegetable machine, filling cup oven, bakery packaging machine,coatingpanformixing, mixer, bakerymixer or spiral mixer, snack cream freezer, ice cream fruit feeder, planetary packaging machine, canning line, soft serve ice like pasta making machine, bottle filling/ A numberofnewequipmentsandinstruments in LivestockFarmforbehaviouralstudies. Behaviour monitoringsystemwasalsoinstalled developed andequipped. Wireless sensorbased climatecontrolroomwas comfort indairycows. and evaluated for effective animal welfare and activity monitoringsystemwerecommissioned computerized milkingsystemandanimal computerized concentratedispensingsystem, Three state-of-the-art housing systems, BPD project. were procuredunder 2 2

● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● Yard, atERS,NDRI,Kalyani. Cattle of corridor Office of Repair/Renovation of NDRI,Kalyani(WB). of metalroads inERS and Carpeting Repairing Kalyani. Repairing ofBoundary WallatERS,NDRI, Bangalore. SRS ofNDRI, Construction ofGirlsHostelat Institute atSRSofNDRI,Bangalore. Development of Farm Road inFarm Area of the at SRSofNDRI,Bangalore. Renovation of“B&E”WingsStudentsHostel Entrance, NDRI,Bangalore. Architectural elevationatInstituteMain Auditorium. Renovation ofD.SundaresanMemorial Farm Section. premises, SeminarRoom/CommitteeRoomin Office and Workshop in rooms of Renovation galvanized wire(8’height)inBlockNo.6. in FarmArea,Repairofexistingfencingwith and providingstoragefacilityinBlockNo.7 Renovation of different sheds and rooms, sheds of theInstitute. Engineering DivisionintoExaminationCentre Conversion of Utility Section of Dairy block. RoomsinAdministrative Halls/Meeting Creation ofnewfacilitiesextraConference Renovation ofInternationalhostel. Internet facilitiesdevelopedinallhostels. functional. On-line ASRBExaminationCentremade of landatNDRI. Forage museum established in around 2 acres students Boys).Allroomsaresingleseated. created. The capacity ofthishostel is 150 New hostel forboy’s i.eNarmada Hostel bathrooms. constructed 52forgirls.Allroomshaveattached made functional. Total rooms (singleseated) New wingofKaverihostelconstructedand establishment ofaTissueCultureLab. facilities besides state-of-the-art equipped with Division renovatedtogiveitamodularlook Molecular BiologyUnitofDairyMicrobiology –– Executive Summary––

xv ndri annual report 2013-14

1 regional stations were established in Bombay Station oftheInstitute.In 1962and1964,two reorganised toserveasthe SouthernRegional already developed atBangalore, theunit was view to consolidate the impressive infrastructure called CentralCattleBreedingFarm.Witha were shiftedtoKarnalatthelocationformerly Institute. Itwas in1955thatitsHeadquarters was expandedandrenamedasImperialDairy established atBangalore in1923.In 1936, it for AnimalHusbandry&Dairying,whichwas metamorphosed from erstwhile Imperial Institute The NationalDairyResearchInstitute process inaharmoniousmanner. industry for orchestrating dairy development among scientists,students,farmersanddairy Institute. The Institutecatalyses close interaction the dairy industry havebeenthehallmarks ofthe level andinfusionofscienceinvarious sectors of development ofhumanresourceatmulti-tier developmental agencies. Scientific achievements, liaison withvariousNationalandInternational on globalbasis.TheInstituteworksinclose frontrankingresearchorganisationas the nation. Overtheyears,ithasgainedprominence for dairydevelopmentprogrammesofthe premier organisation that provides R&D support The NationalDairyResearchInstituteisthe INTRODUCTION H istorical P the researchandtrainingcomponents. Dairy Processingdisciplines tofurtherstrengthen and &Breeding (CAFT)” inAnimalGenetics ICAR, as“CentreforAdvanced FacultyTraining evidenced byrecognitionof theInstituteby capabilities ofthefacultyfromSAUs.Thisis plays an important role in enhancing the teaching State AgriculturalUniversities(SAUs)butalso requiredin ofmanpowerindairying contributor noteworthy thatNDRIisnotonlyanimportant is whichhasnoparallelinAsia.It dairying, of field the in education quality high provides for humanresourcedevelopment.TheInstitute further strengtheningtheacademicprogrammes for Institute the wasconferredto University of Deemed In1989,status functions. management greater operational autonomy inresearch Agricultural Researchwithaviewtoprovide under the wings of the Indian Council of from theMinistry ofAgriculture and brought the Institute’s management was weanedaway conditions thatexistinthoseareas.In1966, development inrelationtotheagro-climatic dairy for support R&D specific region provide and theEasternRegionalStationscontinueto Bombay wascloseddownin1984.TheSouthern However, the Western Regional Station (WRS) at regionalstationsoftheInstitute.and Eastern and Kalyani, respectively, to serve as theWestern erspective

1 ndri annual report 2013-14 2 ndri annual report 2013-14 through 12subject-matterDivisions/Sections. teaching work at the main station is conducted and scientific The station. the at located Head Regional Stationsisadministered throughthe Regional Stations,respectively.Eachofthe educational activities of various Divisions and researchand co-ordinate to are responsible the area of research,academicsandadministration addition toextendingsupporttheDirectorin and itsRegionalStations.TheJointDirectorsin is overallAdministrative HeadoftheInstitute administrative andsupportingstaff.TheDirector technical, scientific, through Directors Joint the of theInstitutearemanagedbyDirectorand The research,educationandextensionactivities shall bethoseasmaydelegatedbytheBOM. and thepowersfunctionofthisCouncil implementing body onAdministrative matters The ExecutiveCouncilisthemaintask is responsible for guiding extensionprogrammes. the respective disciplines. The Extension Council Graduate Faculty,and(iii)theBoardofStudiesin (ii) thePost (i)StandingCommittees, by supported and training.TheAcademicCouncil,inturn,is is responsible forallissues relatingtotheeducation Institute anditsapplication.TheAcademicCouncil responsible forallroundprogressofresearchatthe of this Board. The Research Advisory Committee is Management. TheDirector NDRI,istheChairman Board of body isthe The highestpolicymaking extension activities. main bodiesfocusedatresearch,educationand policy making functions are managed through five the Deemed University patternof the ICAR. The The organisationalstructureofNDRIfollows • • • • • • Executive Council Extension Council Institute ResearchCouncil Academic Council Research AdvisoryCommittee Board ofManagement O rganisational Agriculture) Dairying Ministryof Animal Husbandry& Commissioner, Deptt.of (Animal Husbandry Member (Jt. Director(Ext.Edu.) Member (Director IVRI/IARI) Member (Nominee oftheDG,ICAR) Member ICAR) Nominated bythePresident, (VC ofAgriculturalUniversity Member Apartments, President, ICAR) Body Nominatedbythe Two MembersofGoverning Members Director, By PresidentofICAR) Principal Scientistsnominated Members (HeadofDivisions/ Member Member Chairman S etup

B oard O

f M anagement Dr. R.C.Upadhyay, Honnappagol, Dr. SureshS. Head, ExtensionDivision Director, IARI DDG (AS),ICAR Dr. K.M.L.Pathak Samastipur, Bihar RAU, Pusa, Vice Chancellor, Dr. R.K.Mittal, Phaltan, Maharashtra Research Institute, Nimbkar Agricultural Husbandry Division, Director, Animal Dr. ChandaNimbkar, Dwarka, NewDelhi Vasundhar NAIP, ICAR,A-701, Ex-National Director, Dr. Mruthyunjaya, NBAGR, Karnal Animal Breeding, Principal Scientist, Dr. D.K.Sadana, Mathura –281122(UP) on Goats,Makhdoom, Institute forResearch Director, Central Dr. S.K.Agrawal, NIANP, Bangalore Agriculture Extension, Principal Scientist, Dr. P.Khandekar, NBAGR, Karnal Dr. ArjavaSharma, Head, DCPDivision ABC Division Dr. Y.S.Rajput,Head, (Academic), NDRI Joint Director (Research), NDRI Joint Director Director, NDRI Dr. A.K.Srivastava

Member Secretary Division, Rohtak) (Commissioner, Rohtak Member Commissioner, (FA, ICARorhisNominee) Member Panjab by President,ICAR) Interest tobeNominated RepresentingAgriculture (Non OfficialPersons Member President, ICAR) ICAR, Nominatedby Institute butnotemployedby the Researchworkof Educationist Concernedwith (One EminentAgril. Member President, ICAR) ICAR, Nominatedby Institute butnotemployedby Field ofResearchdonein (One EminentScientistinthe Member from outsidetheNDRI) (Four EminentScientists Members Member NDRI Vice-Chairman Chairman

A cademic C AAU, Anand-388001(Guj) Veterinary Sciencies, Dean, Collegeof Dr. A.M.Thakar, Mathura (UP) Veterinary University, Veterinary Sciencies, Dean, Collegeof Dr. S.K.Garg, Pusa, NewDelhi110012 Anusandhan Bhawan-II, Research, Krishi Council ofAgricultural (EP&D), Indian Astt. DirectorGeneral Sharma, Dr. Kusumakar Joint Director(Research) Joint Director(A), Dr. G.R.Patil, Director, NDRI Dr. A.K.Srivastava, (Admn. &Registrar) Joint Director Sh. J.K.Kewalramani Rohtak Rohtak Division, AS&FA, DARE Tarn-Taran -143303 Progressive Farmer, Sh. GurumukhSingh, Mumbai Ltd., NarimanPoint, Parag MilkFoodsPvt. Chairman, Sh. DevendraShah, Haldwani Kamaluaganja, Foot MillCity,P.O., Ex-Vice Chancellor, Dr. S.N.Maurya, Bhubaneswar Baramunda, Colony, OUAT, C-24/HIG, Ex- ViceChancellor, Dr. K.Pradhan, ouncil

COE FRMS LPM Division each Division) (One SeniorScientistfrom Members (DDG Educationorhisnominee) Member Bangalore-560024 Yelahanka,

ERS, Kalyani Dr. T.K.Datta,Head, Head, SRS,Bangalore Dr. SatishKulkarni, Academic Coordinator Dr. S.K.Kanawjia, Dr. I.K.Sawhney, I/c ABRC Dr. A.K.Chakravarti, Dr. MaganSingh,I/c Dr. ShivPrasad,I/c ABTC Division Dr. R.S.Manik,I/c Head, D.Extn. Dr. K.Ponnusamy, Head, DCDivision Dr. BimleshMann, DM Division Dr. R.K.Malik,Head, DT Division Dr. V.K.Gupta,Head, DES&M Division Dr. K.Datta,Head, ABC Division Dr. Y.S.Rajput,Head, DCN Division Dr. S.Kundu,Head, DCB Division Head, DEDivision Dr. A.K.Dodeja, New Delhi110012 Bhawan-II, Pusa, Krishi Anusandhan Agricultural Research, Indian Councilof (Edn.) General Deputy Director SFS Colony, Yashovana, B1-62,208 Dr. A.K.Gupta,Head, College, Hebbal, Ex-Dean, DairyScience Dr. B.V.Venkateshaiah, –– Organisational Setup––

3 ndri annual report 2013-14 4 ndri annual report 2013-14 –– Organisational Setup–– Member Venkateshwara Member (Maharashtra) Nagpur-440006 Member Director, Member Member Punjab-143204 nominated byDG,ICAR outside ICARsystem, (An EminentScientistfrom Chairman University Member-Secretary Vijayrao Students Member from PGFaculty) (Two Representatives Member Member 25 R esearch A dvisory 1275, 24 Dairy ScienceCollege, Former Director, Dr. P.A.Shankar, Tirupati -517502(A.P) Dr. Y.S.R.Bhavan, Veterinary University, Dean, Sri Reddy, Associate Dr. V.Padmanabhan Seminary Hills, Sciences University, Animal andFishery V. C.Maharashtra Dr. A.K.Mishra, NDRI, Karnal Dr. A.K.Srivastava, ICAR, NewDelhi General (AS), Deputy Director Dr. K.M.L.Pathak, Jaimal Singh,Beas, Satsang, DeraBaba Q-35, Radhasoami Ex-Director, NDRI, Dr. B.N.Mathur, NDRI Deemed Admn. andRegistrar, Joint Director Mr. DeshmukhPavan Ms. RajalaxmiBehera Sr. Scientist,DT Dr. A.K.Singh, DES&M Dr. SmitaSirohi,PS, CCSHAU, Hisar 634, Sector-15-A, Ex-Dean COVAS, Dr. K.L.Raheja, Greater Noida-201306 Green, Sector-PI, Eldeo Residency T-23, GroundFloor, Ex- DirectorNIANP, Dr. KhubSingh, Bangalore-560070 C th MainBSKStage, ommittee th Cross,

Division Member Secretary India Information), Haryana, NDRI Members Chairman Member Secretary Member E xtension C Head, DairyExtension Dr. K.Ponnusamy, Extension, Govt.of Directorate of Director (Farm & Dairying,Govt.of of AnimalHusbandry Director General,Dept. Ministry ofAgriculture (Crops), Govt.ofIndia, Additional Comm. Dr. D.P.Malik, ERS, Bangalore Dr. T.K.Dutta,Head, DCB Division Dr. AvtarSingh,PS, Scientist, D.Extn. Mrs. RituChakravarty, & I/cATIC Dr. P.S.Oberoi,PS & I/cLPMLRC Dr. ShivPrasad,PS PS, DCP Dr. O.K.Hooda, PS, DES&M Dr. B.S.Chandel, Sr. Scientist,DT Dr. A.K.Singh, PS &I/c,ABRC Dr. A.K.Chakravarty, Education), ICAR DDG (Extension Director (Academic), Dr. G.R.Patil,Joint Director (Research), Dr. G.R.Patil,Joint Director, NDRI Dr. A.K.Srivastava, Joint Director(Res.) Dr. G.R.Patil Pusa NewDelhi-110012 Road (Opp.Todapur) Dev PrakashShastri Complex, CGBlock, Office, NASC IFPRI, NewDelhi Director, SouthAsia, Dr. P.K.Joshi, ouncil

Financial Outlays&Expenditureduring2013-14 Plan andNon-planin2013-2014was`14531.09 ` for PlanandNon-plantheyear2013-2014was The financial outlays in terms of actual expenditure **Proposal forrevivalof12vacantpostsaforesaidcategorysentto theCouncil. *10 postsofUDCsareexcessfromthesanctionedstrengthpost of UDCs. Position ofManpoweratNDRI,KarnalanditsRegionalStationsason31.03.2014 Total Supporting Administrative (GroupB&C) Technical Administrative (GroupA&B) Scientific Total Plan Non-Plan Type ofPosts Head 14501.47 lakhs and the sanctioned budget for The RevenueReceiptsoftheInstituteandRegionalStationsforyear2013-2014were Budget 1466 755 127 355 35 194 D/o Expenditure Approved by Existing FINANCIAL OUTLAY 1112 550 *115 260 30 157 In position F

` 14531.09 inance Regional Stations. for strengtheningofDeemedUniversity/KVK and outlays financial the include figures These lakhs. 364 205 **22 95 05 37 Vacant ` 14501.47 14531.09 13012.00 Budget 1519.09 46 11 Nil 15 - 20 Proposed ** Additional Expenditure 1431+46 755+11 127+00 355+15 - 194+20 Additional Sanctioned + Total ` 899.50lakhs. Expenditure 14501.47 12982.77 1518.70 1182+46 550+11 115+00 260+15 - 157+20 Additional Existing + (` inlakhs)

5 ndri annual report 2013-14 6 ndri annual report 2013-14 2 • • • • Higher ProductivityandFertility for Karan Fries Cattle Multi-trait Evaluationof Technologies of EmergingReproductiveandMolecular of SuperiorGermplasmbyApplication through Identification and Dissemination MilchAnimals of Genetic Improvement

were 349.10%, 349.05%, 316%, 32.75%, 53.55%, 32.75%, 316%, 349.05%, 349.10%, were ST-AM by traits FSP and AFC (%), SNF yield, SNF (%), fat yield, fat 180DMY, PY305MY, The improvement in heritability 0.156 ±0.058and0.0100.047, respectively. 0.010, 0.206 ± 0.010, 0.349 ± 0.091, 0.339 ± 0.094, models (ST-AM)were0.238±0.087,0.208 estimates ofthesetraitsfromsingle-traitanimal heritability Thecorresponding software. single trait-sire model (ST-SM) using WOMBAT from± 0.031and0.0030.015,respectively 0.050 ±0.024,0.1540.043,0.1110.038,0.108 were 0.053±0.024,0.0500.023, and FSPtraits milk yield (180DMY), fat yield, SNF yield, AFC using 180-daymilkyield(PY305MY), yield (FL305MY),predicted305-daymilk milk 305-day lactation first of heritability The conditions. been recorded for evaluation of bulls under field day milk yield dataon598KaranFries have calves wereborninthefarmers’ herds andtest A total of 1167 (622 males and 545 females) KF 2012. December, to up conditions field under as a result 47.04% conception rate was obtained and KF in performed were A.I 3562 of total A is beingcompiledonX–XIIIsetsofbulls. Information %. 12.28 and 5.03 between ranged 4348-6861 Kg.TheEPDofselectedmalecalves lactation yield of reserved KFmales ranged from for futurebreeding.The dam’s best305days conformity forselectionofyoungmalecalves yield, breed characteristics and physical basis ofEPDanddam’s best 305dayslactation A total of 18 KF male calves were reserved on the kg withanaverageof5528kg. 4158 and8648 day milkyieldrangedbetween kg) and average EPA (4114 kg). The best 305 and 12.29% higher than the herd average (4250 and average EPA as 4620 kg which were 13.90% with anaverage305-daymilkyieldas 4841 kg selected as elite cows for nominated matings were (29.56%) cows Fries Karan 68 of total A RESEARCH ACHIEVEMENTS D

of FL305MY, airy C attle • • breeding tractofJaisalmerin Rajasthan. nineteen heifers/cows werepurchased from genetic baseofTharparkar herd attheInstitute, the widen To respectively. 88.1%, and 69.0 3.5, 79.08, 1792.65, 62.64, 3.13, 3.48, were (%) integrity acrosomal and (%) HOST (%), abnormalities (%), live (millions/ml), concentration sperm (%), motility individual activity, mass volume, ejaculate Theaverage assessed forsemenquality. In all29ejaculates of4breeding bulls were farmers forgeneticimprovementinthefield. frozen semen were sold to various agencies/ developmental agencies.Atotalof215doses semen weresupplied/soldtothefarmers/ were producedandonethousanddosesoffrozen Around thirtythousand doses of frozensemen Seven bullswereunderregularsemencollection. Germplasm Multiplication and Dissemination of Tharparkar B

for FLL. magnitude havinghighstandarderrorsexcept traits wereindesirabledirectionbutlower average). Theannualgenetictrendsofthese herd of (0.13% FCI for days -0.59 and average) herd of (-0.11% AFC for days -1.08 average), 0.58 days for first lactation length (0.16% of herd average), herd of (0.09% yield milk lactation total for kg 3.44 average), herd of (0.15% yield method were4.85kgfor305-day/lessmilk traits (period1992-2009)estimatedbyBLUP The annual genetic trends for the first lactation the estimateofST-SManalysis. to compared respectively 233.33%, and 30.76% on improvedestimateofheritability. trait animal model of traits combinations based 180DMY traits was found superior to other four- and FSPwitheachofFL305MY,PY305MY the modeloftraitscombinationSNFyield,AFC heritability. Amongfour-traitanimalmodels, combinations basedonimprovedestimateof evaluation amongothersconsidered three trait traits wasobservedtobeabettermethodof with eachofFL305MY,PY305MYand180DMY traits combinationAFCandFSP(ascovariate) animal modelof combinations. Thethree-trait of heritabilitycomparedtoothertwotrait better methodbasedonimprovedestimate and 180DMY traits was observed to be SNF yieldwithanyoneofFL305MY,PY305MY The two-trait animal model of traits combination reeding Karan Friescattle.Incaseof Sahiwalcattle,allthe of theanimalspossessed12G/13G genotypein 3.5% only while genotype 13G/13G possessed screened animals the of 96.5% that revealed within exon1regionofFEZL gene.The results cattle weregenotypedfor12G/13G polymorphism of 114KaranFriescattleand111Sahiwal A total for 12G/13Gheterozygousgenotypes. and twopeaksat 125and128basepairs genotype single peakat128basepairs for 13Ghomozygous genotype was a single peak at 125 base pairs; The expected product size for 12G homozygous gene usingFAM dye conjugated forward primer. was utilized to identify allelic variants withinFEZL residues (12G/13G) in 12or13Glycine resulting The threenucleotideINDELpolymorphism in the5’flankingregionofFEZLgene. sites werepredicted factor binding transcription among bovines.Atotalofsixputativesitesfor cattle showedhighlyconserved natureofthegene promoter ofFEZLgeneinSahiwalandKaranFries of ~0.7kb5’ flankingregionincluding basal characterization Sequence human. with 94% and Karan Fries cattle was 99% with that of The percentidentityofFEZLgeneSahiwaland and KFcattle. within exon1regionatposition182inSahiwal Glycine residue and aserine to proline change FEZL genewasfoundtohaveanadditional conceptualized amino acid sequence of bovine each of intron 1and intron 3, respectively. The exon 1,whiletwo mutations wereobserved within including threeINDELS were found to be within the eightnucleotidevariations,fourmutations X Bosindicus(114KaranFries)cattle.Among Bos taurus,indicus(111Sahiwal)and were observedamongFEZLgenesequencesof three INDELSandfivesubstitutionmutations A totalofeightnucleotidevariationsincluding JX312831 andJX312832). respectively (NCBI-GenBankaccessionnumbers and 3.789kbinSahiwalKaranFriescattle, kb of primers indicated that the genes span over 3.772 BTA 22.Sequencecharacterizationusingtensets in immunityandFEZLgenehasbeenmappedto finger like protein (FEZL) plays an important role Karan Friescattle:Forebrainembryoniczinc of polymorphismFEZLgeneinSahiwaland Molecular characterization andidentification Sahiwal, KaranFriesCattleandMurrahBuffaloes of PolymorphisminMastitisRelatedGenes Identification and Characterization Molecular Bos taurus Bos taurus Fries cattleand147Sahiwalcattle. SNP in148Karan the to type enzyme HpyCH4V pairs forS100A8fragment-IIIandtherestriction PCR-RFLP assaywasdeveloped using theprimer in ordertotypetheanimalsforassociation study. assay codon wasutilizedtodevelopgenotyping The SNP locus at position -2 bpupstream to start binding siteswerepredictedwithinthisregion. bovine sub-species. 18 putative transcription factor revealed 17nucleotidechangesamongdifferent of S100A8genewassequencecharacterizedwhich and swamp buffalo. About 1 kb 5’ flanking region cattle, 99% identity with yak, 97 with both riverine were found to have 100% identity with DNA sequenceofSahiwalandKaranFriescattle and two from exonic regions. TheS100A8 coding variations ofwhichsixwerefromintronicregions nucleotide different bovinespeciesrevealedeight sequenceof ComparisonofS100A8gene cattle. gene wasgeneratedinSahiwalandKaranFries (Calgranulin) S100A8 of regions flanking 5’ and kb sequencedatacovering entirecodingDNA Karan Fries cattle:A total of2.036kband 2.080 characterization ofS100A8 gene inSahiwal and Identification ofpolymorphismand animals. found tobeassociatedwithmastitisinthestudied homozygotes. FEZLgeneticvariantswerenot animals genotyped were found to be 13G/13G and PCR-RFLP analysis was performed using 12 primers of sets 16 using amplified were sequence Murrah buffaloesfromNDRI herdandcoding was isolatedfromwholeblood of200lactating with mastitisinMurrahbuffalo: GenomicDNA Lactoferrin genevariantsandtheirassociation in cattlewasobserved. S100A8 genotypesandincidenceofclinicalmastitis of association significant No cattle. Fries Karan while itwas0.973,0.02and0.007,respectivelyin inSahiwalcattle 0.884, 0.102and0.013,respectively genotypes at-2(A/G)SNPlocuswasfoundtobe The overallfrequencyofGG,GAandAA –– DairyCattleBreeding –– Bos taurus

7 ndri annual report 2013-14 8 ndri annual report 2013-14 –– DairyCattleBreeding –– PCR-RFLP ofExon12lactoferringeneusingHpy188Irestrictionenzyme exon 12,13and17withthreegenotypes(EE, Hpy188I RE exhibited polymorphic pattern in of C and D allele were0.73and0.27,respectively. frequency of 0.62, 0.22 and 0.16. The frequencies three genotypes(CC, CD and DD) with respective while BccIshowedpolymorphicpatternwith exon 7.HindIII exhibited monomorphic pattern, endonuleases havebeenusedfordigestionof and 0.19,respectively.BccIHindIII restriction 0.375. ThefrequenciesofAandBalleleswere0.81 (AA and AB) with respective frequency of 0.625 and polymorphic patternforexon6withtwogenotypes FokI restrictionendonucleaseexhibited acids substitutions. 52 werefoundincodingregionleadingto23amino total of 91 changes in Murrah buffalo, out of which with referencesequenceofBostaurusindicateda restriction enzymes.Multiplesequencealignment polymorphism incontig2withtwogenotypes Hpy substitutions ofL184F,L265P, S235TandR337H. water buffalo,respectivelyleading toaminoacid G1110A wereobserved with Chinese and Indian T794C and T975C and two changes at T703A and C550T, at changes nucleotide sequences, three comparison of 1122bpcoding region withBubaline region, leadingto9aminoacidssubstitutions.On out ofwhich18SNPsweredetectedincoding changes, of70nucleotide alignment revealedatotal covering completeCD14gene.Multiplesequence buffaloes were amplified using six sets of primers of GenomicDNAfrom100lactatingMurrah is oneofthecandidategenesformastitis. A total buffalo: CD14geneinMurrah geneticvariantsof of Molecular characterization andidentification Lane 7,11,13,14,16:ABGenotype Lane 15:AAGenotype 188I restriction endonuclease exhibited Cluster ofDifferentiation14(CD14)gene Lane M:DNALadder(50bp) Lane 1-6,8-10,12:BBGenotype combined genotypes. susceptible to mastitis as compared to other AACCHH genotyped animalswerefoundmost found leastsusceptibletomastitis,whereas, AACCGG combinedgenotypedanimalswere to mastitisascomparedothergenotypes. genotyped animalswerefoundlesssusceptible incidence ofclinicalmastitis.AA,CCandGG association ofexons6,7and17genotypeswith significant revealed analysis (χ2) Chi-square and 0.29,respectively. G and H alleles were 0.25, 0.75, 0.49, 0.51 0.71 (HH) inexon17.ThefrequenciesofE,F,M,N, in exon13 and 0.56(FF),MM(0.20),MN(0.58),NN(0.22) in exon 12 werefound to be 0.06 (EE), 0.39(EF) GG, GH,HHofexon17).Genotypicfrequencies EF, FFofexon12,MM,MN,NN13and (KS) and Karan Fries (KF), respectively. In twins Swiss Karan Sahiwal, in 0.92% and 0.83 0.19, was which 0.77%, was rate twinning overall took placeinthreedifferent geneticgroups.The total of18,608calvingincluding 143twinbirths 1969 toDecember2011fortwinningshoweda The calving records scrutinized from January performance: twinsandtheir born Natural of ReproductiveDisorders of Cattle,BuffaloesandGoatsforEarlyDetection Animals Pre-Pubertal of Screening Cytogenetical with BBgenotypes. found to be less susceptible as compared to animals affected animals. AB genotyped animals were 0.53. BB genotype was more prevalent inmastitis (AB and BB) with respectivefrequency of0.47and and in0.59(GG),0.23(GH)0.18

remained intheherd. So only 10% of the total entered in production and age orbeyondmainlyasexperimental purpose. of years 5 to stayed 19% only Thus, age. of years 3-5 within 30.4% to rose which age, of years 1-2 the remaining animals, 7.2% were disposed within % were disposed within 2-8 month of age. Among of age out of which 50% were still births. Around 13 ET borncalvesweredisposed/diedwithin1month The study of disposal pattern revealed 20.8% of the the patterns. the putativesiredidnotrevealsimilarityin observed betweentruesireandprogenywhereas almost monomorphic pattern.Band sharing was TGLA 122primers.Therestwerefoundtoyield revealed polymorphismwithINRA023and testingwith micro-satellitesin targeted samples had normal cytogenetic profile. The paternity carrier), respectively.TheprogenyofrestETborn and 10:1 (translocation carrier: non translocation translocation carriers, corresponding toratioof3:1 sired bybull–1and1progenybull–2as screeningshowed4progenyThe cytogenetic was onechimaeric(freemartin),andsterile. There confirmed. be is translocation in involved confirmed by C-banding, however, the autosomes carriers. Thetranslocationofautosomeswas born animalsrevealedtwomalesastranslocation freemartin). ThecytogeneticscreeningoftheET available intheherd(4females,2malesand1 Amongtheseonly7werephysicallymaterial. 37 males)producedthroughETwerethestudy technology: Animals bornthroughembryotransfer breeds. females at18monthsbodyweightofallthethree effect at6monthsbodyweightinmalesand the three breeds. Twinning showed non-significant problems andsurplus inall death, reproductive The mainreasonsofdisposalmaletwinswere in twinsthansingletonsallthethreebreeds. KF, respectively.Themortalityratewashigher and KS Sahiwal, in 6.58% and 3.93, 5.48, was were 2.75, 12.90, and 2.58% and that of abortions However, insingletonsoccurrenceofstillbirths respectively. KF, and KS Sahiwal, in 2.44% and 1.82, 16.67, was abortions of that and 18.29% occurrence ofstillbirthswas0.00,11.82,and A totalof69calves (32 females and ranged between 2733 kg in first lactation to 4341 kg. best lactational milk yield ofeliteMurrah buffaloes The average. herd the than higher 47.60% was Murrah buffaloes increased to 3151.29kgwhich animals. The average lactationmilkyieldofelite elite as identified were buffaloes Murrah 37.58% using semenofthreeProvenMurrahBulls.About in theherdwasfollowed for nominatedmating mating underXVSet.Thebreedingprogramme test for selected were bulls breeding five which The center proposed seven Murrah bulls out of kg. 4090 to lactation first in kg 2747 from ranged 305 dayslactationmilkyieldofreservedmales Thedam’sbest breeding. reserved forfuture A totalofteneliteMurrahmalecalveswere increased to7.80kgand4.70kg,respectively. were average herd and wet The 41.72%. was The overallfemaleconceptionrateintheherd of buffaloeshasbeenestimatedas131.19days. and 18.0months.Theaverageserviceperiod 16.7 to reduced significantly has bulls Murrah young the of collection first at age and training 31.95 and 42.44month.Average ageforinitiating service and age at first calving of buffaloes were first at age Average respectively. kg, 4341 to bulls hadrangedfrom3805to4470kgand2971 lactation 305dayorlessmilkyieldofthetest test bullsattheinstituteherd.Thedams’best was followed for test mating using semen of The breedingprogrammeintheMurrahherd (Institute Unit) Network ProjectonBuffaloImprovement semen forInstituteextension/otheractivities. agencies throughsaleinadditiontosupplyof both setsweresuppliedtofarmers/developmental thousand doses of bulls of high genetic merit under During theperiod(Jan-Dec,2013),aroundthirteen Pantnagar and Bhiwani for AI at those centres. GLF Hisar,GADVASU,Ludhiana,GBPUA&T, semen dosesweresuppliedtoDRUunitsat second setwasstartedfromOctober,2013and seven bullsofSet-II.AIwithsemen twenty fourthousanddoses were produced from were producedfromthreebullsofSet-Iandaround Around twelvethousanddoses of frozensemen Indigenous BreedProjectonSahiwalCattle –– DairyCattleBreeding ––

9 ndri annual report 2013-14 10 ndri annual report 2013-14 –– DairyCattleBreeding –– comfort zone. to compared as CHSZ under 1.3% and 0.6% rate andpregnancywere decreasedabout Expected Fertilizing Ability (EFA) for conception as compared to CZ in Murrah buffaloes. The CHSZ under 0.5% and 0.3% about decreased for conception rate and pregnancy rate were period was found 2 days higher, while EBV The ExpectedBreedingValue(EBV)forservice compared tocomfortzone. as zone stress heat under for 1% and 0.42% rate ofMurrah buffaloesweredecreasedabout Ability (EFA) for conception rate and pregnancy CZ inMurrahbuffaloes.TheExpectedFertilizing about 0.21% and 0.4% under HSZ as compared to conception rateand pregnancy ratewere decreased period wasfound1dayhigher,whileEBVfor forservice Value(EBV) Breeding The Expected CHSZ. withperunitincreaseinTHIvalueunderrate pregnancy 7% and rate conception 9% in decline There wasincrease of28days service period, month were found as CHSZ for conception rate. pregnancy rate,whereasJune,JulyandAugust Stress Zone(CHSZ)forserviceperiodand Heat The months,MayandJune,werefoundasCritical increased theserviceperiodinMurrahbuffaloes. the monthofMarchto75inApril buffaloes. TheincreaseinaverageTHIfrom68 Murrah in traits fertility for 75 as identified was suitable heatstressmodel.ThethresholdTHI best the as identified was 6 model THI (HSZ). September were identified as heat stress zone (CZ), whileApril,May,June,July,Augustand zone comfort as identified were December and January, February,March, October, November over 20years(1993to2012).Themonthssuchas 1379 lactationrecords spread was studiedusing relation to heat stress in 581 Murrah buffaloes period, conceptionrateandpregnancyrate)in Genetic evaluationoffertilitytraits(service to HeatStressinMurrahBuffaloes Genetic Evaluation ofFertilityTraitsinRelation period. and dairydevelopmentorganizationsduringthe supplied 62,054doses of frozensementofarmers has centre The bulls. test five from semen frozen The NDRIcentrehasproduced60,915dosesof 9 breeding bulls belonging to the 14 centers was5929and5339,respectively.Asmanyas females andthebreedableatdifferent conditions. The total herd strength of registered field under bulls of evaluation for recorded been herds andperformancedataon211daughtershave Murrah buffalocalveswereborninthefarmers’ female) 565 and male (660 1225 of total A (48.70%). lowest wasobservedinthevillageKherimansingh conception rate was observed in Darar (59.85%) and highest the villages, the Across (47.05%). May of month the in was lowest the and %) (54.76 October highest conceptionratewasobservedinthemonthof as a result 51.08 % conception rate was obtained. The Buffaloes under field conditions during 2013-14 and A total of 4419 AI wereperformed inMurrah Unit) Network ProjectonBuffaloImprovement(Field has been transmitted to their progeny. has beentransmittedtotheir progeny. bulls, revealed translocation of chromosomes, which manipulation ofembryoswere raisedandusedas anomaly. Monozygotictwin malesbornthrough numerical andstructuraldetailsdidnotshow Evaluation ofchromosomes among thesemajoritydidnotreachbreedingage. 67 casesofcalvesbornwithETwereexamined, developed ovaries.InNDRIcattleyardrecordsof detected. Oneofthesedied,autopsyshowedunder Three buffalo heifers with 49, XO conditions were monitored for their performance behavior with age. expected tohave reproductive problems,and are are which X-chromosomes, fragile 30% than higher with cases 34 were There 41.89%. to 3.95 of youngcalves(81)andfragilesitesrangedfrom with DNA primers. Chromosomes were evaluated confirmed and detected were (FSs) sites Fragile of repeat breeding, irregular cycle and abortion. observed inSahiwalcattlefemales(27)withhistory latter israrecase.Fragile-Xchromosomeswere ambiguous externalgenitaliawereobserved.The XX) andonechimaeric(60,XX/60,XY)goatswith interesting results.Suchasthreeintersexes(60, anomalies and theireffect revealed useful and typesofchromosomalStudies ondifferent Goats Reproductive ProblemsinCattle,Buffaloand Chromosomes forDetectionofGeneticaland Associated TechniquesforCharacterizationof Fluorescent for AIduringtheyear. in situ in Hybridization (FISH) and (FISH) Hybridization of AIbulls(51)for th set were used set were used birth. Karan Kirtiwere born butdied due to premature in thehistoryofInstitute.Two more clones of the distinctionofbeinghighestmilkproducer which has clone ofabuffalonamed‘KaranKirti, 2013. Purnima,whichsurvivedfor21days,wasthe born throughnormalparturitiononSeptember6, ‘Purnima’, a cloned buffalo calf weighing44kg was Birth of‘Purnima’,aClone‘KaranKirti’ Cloning Reproductive andMolecularTechnologies Germplasm byApplicationofEmerging Superior of Dissemination and Identification Genetic Improvement ofMilch Animals through to 12recipients,onecalfweighing 55kg,which embryos produced from fresh semen-derived cells cloned embryos. Followingtransferof24cloned 3 but notthatof DNMT3a differed in cells and in abundance of HDAC1, DNMT1, P53 and CASPASE mRNA The relative embryos fromdifferentbulls. differed significantly(P<0.05)amongcloned H3K27me3 butnotthatofH3K9/14acandH4K5ac epigenetic statusrevealedthatthegloballevelof cell sourcessuchasskin.Examinationofthe fromconventional obtained semenorthose thawed produced using cells obtainedfrom fresh orfrozen- embryos among index,were comparable apoptotic by asindicated rateandquality, The blastocyst embryos. hand-made cloningforproducingbuffalo origin. Thecellswereusedasnucleardonors for vimentin indicatingthattheywereofepithelial The cellsexpressedcytokeratin-18,keratinand 10 yearsearlier,andwereestablishedinculture. semen of4buffalobulls,3whichhaddiedover Somatic cellswereisolatedfromthecryopreserved Somatic Cells Semen-Derived from Cloned Calves Birth of ‘Purnima’, aCloneof‘KaranKirti’ A nimal B iotechnology higher forinter-speciesthan thatforintra-species and the apoptotic index was significantly (P<0.05) cell number (TCN) was significantly (P<0.05) lower total the However, buffalo. wild from fibroblasts species clonedembryos produced usingskin inter- and buffalo domestic from fibroblasts skin the intra-species cloned embryos produced using blastocyst rate were significantly different between domestic buffalo.Neitherthecleavagenor from fibroblasts skin of that to compared lower (P<0.05) significantly was rate proliferation cell the and higher (P<0.05) significantly was buffalo doubling timeofskinfibroblastsfromwild of were they but notkeratinandcytokeratin-18indicatingthat of wildbuffalowerefoundtoexpressvimentin oocytes. Somatic cells derived from the ear skin bubalis (Bubalus cells anddomesticbuffalo Hand-made cloning using wild buffalo somatic somatic cell nuclear transfer (iSCNT) through producing wild buffaloembryos by interspecies In astudyaimedatexploringthepossibilityof (Bubalus bubalis arnee) andOocytesfromDomesticBuffalo Wild Buffalo(Bubalus Using DonorCellsfrom Production ofInter-Species Cloned Blastocysts also beusedforrestorationofendangeredspecies. which hasimmense economicimportance, andcan restoration of highly precious progeny-tested bulls, for way the pave findings These reprogrammed. be to cells these of ability the confirms offspring fresh and frozen-thawed semen to produce live of cellsderived from 12 hafterbirth.Theability born wasseverelyunderweight(17kg),anddied one of which aborted in the first trimester; the calf derived cellsto10recipients,2becamepregnant, 20 embryos produced from frozen-thawed semen- through normal parturition. Followingtransferof is now 6 months of age and is normal, was born ‘Swarn’ withfostermother broblast origin. The population The origin. fibroblast ) )

11 ndri annual report 2013-14 12 ndri annual report 2013-14 G TOPO-Bu-NANOG of μg) 3.20 : Z μg, 1.60 : Y Subsequently, different concentrations (X: 0.8 μg, green fluorescent protein (GFP) in both cell types. buffalo BAFcellsandshowedexpressionof developed. Initially, TOPO-Bu-NANOG G was geneconstruct and pCAGGS-Bu-NANOG eukaryotic expressionvector(pCAGGS-PGKneo) buffalo NANOGORFwasalsoclonedinother harboring buffalo NANOG ORF insert. Further, construct wastransfectedinCOS-1 TOPO-Bu-NANOG G expression vectori.e.CT-GFP-TOPOfusion. Buffalo NANOGORFwasclonedinaeukaryotic Stem Cells FGF2 inBuffaloFetalFibroblastsandEmbryonic Over-expression ofBuffalo NANOG, LIF and Stem Cells H3K18ac was significantly (P<0.05) lower in inter- species clonedembryos.The globallevelof intra- forcorresponding thanthat inter-species significantly (P<0.05) higher for vitrified-warmed was index apoptotic the different, significantly as indicatedbytheirre-expansionratewasnot cryosurvival rateofbothtypesclonedembryos, Pulled straws (OPS) and warming, althoughthe Open- in vitrification Following embryos. cloned –– AnimalBiotechnology –– vector pAcGFP1N1 and CT-GFP-TOPO fusion, ORF wereclonedineukaryoticexpression FGF2 NANOG over-expression studies. BuffaloLIF and for cells ES and cells (BFF) fibroblast fetal buffalo The constructswerethentransfectedinto line. NANOG was transfected into CHO-K1cell +1 ,

pCAGGS-Hu-NANOG andpCAGGS-Bu- +1 construct was developed

cell lineand +1 +1 with NANOG, LIF and FGF2 construct aloneorin with NANOG,LIFandFGF2 cells cultured onthetransfectedBFFfeeder cells of the pluripotencygenes was upregulated inES and FGF2growthfactors.The relativeabundance transcripts in buffalo ES cells in absence of LIF of theNANOG, Oct4, Sox2, c-Myc and FoxD3 construct intoBFFcellsshowedhigherabundance Transfection ofNANOG,LIFand FGF2gene growth factors. or presenceofbothLIFandFGF2 on non-transfectedBFFfeedercellsintheabsence alone orincombinationthantheES cells grown buffalo NANOG,LIF,andFGF2geneconstruct grown onbuffaloBFFfeedercellstransfectedwith The EScellcolonyareawashigherincells factors. growth cells inpresenceofLIFandFGF2 human orbuffaloNANOGtransfectedBFFfeeder FoxD3) wereupregulatedintheES cells grown on pluripotency genes (i.e. NANOG, Oct4, c-Myc and as comparedtocontrolBFFfeedercells.The either humanorbuffaloNANOGgeneconstruct cells grown on BFF feeder layer transfected with produced byiSCNT. demonstrate thatwild buffalo embryos can be two typesofembryos.Inconclusion,theseresults of DNMT1 whereas that in inter-speciesthanintra-speciesembryos, higher, thatofP53 DNMT3a andCASPASE3 was significantly (P<0.05) cloned embryos. The expression levelofHDAC1, species cloned embryosthanthatinintra-species area (μm transfected buffalo BFF feeder cells. The colony cell line.EScellswereculturedon CHO-K1 into respectively, andthenboth weretransfected 2 ) wassignificantlyhigher(p<0.05)inES was significantly (P<0.05) lower was similar between the was similarbetween was reducedinbuffaloNANOGG Cdx2, Gata6,Gcnf,Tcf3,p53,SMAD1andSMAD2 double construct. The relativemRNA abundance of were furthercharacterized byimmunostaining undifferentiated MSCs. Pancreatic islets-likecells No expressionwasdetected forthesegenesin NGN3, PDX-1,ISL-1,c-KIT, THY-1andGLUT-2. pancreatic endoderm-specific genes i.e., IGF-1, SST, cells werecharacterized by amplificationof mercaptoethanol (20 µM). Pancreatic islets-like pentagastrin (10nM), retinoic acid (10µM) and (10 mM), activin-A (2 nM), exendin-4 (10 nM), with glucose in the presence of nicotinamide these wereincubatedinconditionedmedium stem cells(MSCs)topancreaticisletslikecells, In order todifferentiate goat mesenchymal Mesenchymal StemCells from cells β Secreting Insulin of Generation and FGF2growthfactors. on transgenic BFF feeder layer in absence of LIF FGF2 geneconstruct(s).TheEScellscanbegrown ES cells were generated carrying the NANOG, LIF, cells. Thus, buffalo transgenic BFF feeder layerand with NANOGgeneconstruct than thecontrol ES transcripts werehigherintheEScellstransfected Sox2 (p>0.05),c-Myc(p<0.05)andFoxD3(p>0.05) other construct transfected ES cells. Similarly, construct thanthecontrol ES cells, mock and (p<0.05) inEScellstransfectedwithNANOG higher significantly was transcript NANOG of other construct transfected ES cells. The expression control, mockand the construct than gene FGF2 higher (p<0.05) in the ES cells transfected with oftheFGF2transcriptwasrelative abundance of thenon-transfectedandmockEScells.The LIF, andpooledconstructswashigherthanthat in ES cells transfected withbuffalo NANOGG ES cells.TherelativeabundanceofLIFtranscript size oftransfected EScells thanthenon-transfected and FGF2geneconstructsimprovedthecolony FGF2 growthfactors.Inthepresentstudy,LIF transfected EScellsinthepresenceofLIFand gene construct was higher (p>0.05)thanthenon- cells withNANOG,LIF,andFGF2pooled However, the colony area of all transfected ES cells inabsenceofLIFandFGF2growthfactors. not significantlydifferentfromthecontrolES was constructs gene (24.02±5.13%) pooled and (12.90±5.64%) FGF2 with cells ES transfected of area colony the while (26.34±6.04%), cells ES construct was higher than the non-transfected (28.83±6.80%) LIF and (29.42±6.54%) NANOG The colonyareaoftransfectedEScellswith cells thanthenon-transfectedEScells. +1 transfected ES transfectedES +1 , levels between0.18-1.43ng/ml/24h/2.0x10 insulin-expressing clonessecretedinsulinatvarying Western BlottingandELISA.ThepAcISUBC confirmed byRT-PCR,Immunocytochemistry, cell clones.ThehINSexpressionincloneswas G418 drugfor3weekstoobtainpositivetransgene line byliposomaltransfectionandculturedwith gene constructwastransferredintoaBuMECcell recombinant human insulin in BuMEC cell line. The (buBLG) promoterwasusedtodriveexpressionof mammary gland-specific buffalo beta-lactoglobulin consensus cleavagesequences(Arg-X-Lys-Arg).A by overlapextensionPCRforintroducingfurin was clonedfromitsgenomicgeneandmutated Human pro-insulincDNA validated. (BuMECs) its functioninbuffalomammaryepithelialcells gland specific expression vector was generated and demand ofhINSinmedicaluse.Amammary a largescalefromtransgenicbuffalotomeetthe developed toproducehumaninsulin(hINS)on A mammary gland-specific expression vector was Mammary EpithelialCells Human Insulin Gene Expressed in Buffalo Transgenesis were alsosuccessfullyestablished. COS-1 celllinesexpressingrBuLIFfusedwithGFP be utilizedacross different labs.Stablytransfected expression inanymammaliancelllinewhichcan expression constructswerepreparedforits HQ616665).Mammalianaccession number (NCBI GenBanksequenced andcharacterized LIF of buffalo origin was successfully cloned, System Leukemia Inhibitory Factor(LIF)in Eukaryotic Molecular CloningandExpressionofBuffalo specific protein. of Pdx-1,insulinandIslets-1 and westernblotting isoforms. Out of eight isoforms, buffalo IFN-τ1 was IFN-τ buffalo distinct 8 for encoded that variants of therecombinantcoloniesgave 13distinctcDNA screening and product amplified the of Cloning sequence. IFN-τ bovine known against designed primers gene-specific using performed was PCR extraction fromprimarytrophectodermalcells,RT- relatively predominant isoforms, total cellular RNA pregnancy signalling molecule. To identifythe first the as pregnancy of establishment as well as implantation in role significant a performs IFN-τ Buffalo Interferon-Tau Expression andPurificationofRecombinant –– AnimalBiotechnology –– 6 cells. cells.

13 ndri annual report 2013-14 14 ndri annual report 2013-14 genes associated with biological processes, cellular genes associatedwithbiological processes,cellular of highandlowfertilebuffalo bulls.Important were founddifferentiallymethylated insperm with another55genescovered under CpGislands microarray. A total of96individual genes along buffalo (Bubalusbubalis)CpGisland/promoter methylation changesusingacustomdesigned180K different fertilitystatuswasinvestigatedforglobal pattern inspermatozoa of buffalobulls of ofspermatozoa. TheDNAmethylationability as oneofthereasons for compromised fertilizing epigenetic carriageofspermatozoaissuspected the qualityofsemenbeforefreezing.Differential quality control measures applied whiledeciding experienced inalargeAI trial inspiteofstringent of breedingbullsis Wide variationinthefertility Epigenetics ofSpermDNA of betterARTprotocols. to development which couldtranslate rate ofIVF This brings inanewstrategy toenhancesuccess of blastocysts as well as better blastocystquality. in theIVFprocedureresultedhighernumber dose of E-64whileculturingtheoocytesforIVM competence ofbuffalooocytes. Using anoptimized BCL2 ratioeventuallyincreasingthedevelopment expression ofpro-apoptoticgenesandBAX/ dependent apoptosis process bydecreasing the rescue thecumuluscellsundergoingmitochondria B bysupplementingE-64inIVMmediumcan the investigation suggest thatinhibitionof cath TUNEL assay.Theresultsof the as evidencedby supplementation resulting inlower apoptosis rates genes. The BAX to BCL2 ratio also reduced on E-64 cath B expression along withotherpro-apoptotic (E-64) intheIVMmediumreduced inhibitor of cathepsinexpressionusingcysteineprotease the course ofin vitro responsible for deterioration of oocyte health in factor key a as identified was gene B cathepsin of in majorityoftheoocytes.Increasedexpression how thedevelopmentabilitycould be maintained the buffalooocyteswasstudiedtounderstand The apoptosiseventincumuluscellsurrounding Oocyte Genomics using anti-HISantibody. blot western by confirmed was obtained IFN-τ buffalo Therecombinant thiogalactopyranoside. β-D-1- isopropyl by induced was form soluble in coli strain.Expression of the recombinantprotein and wasexpressedincompetentBL21(DE3)E. without signalsequencefrompJETcloningvector It was subcloned inexpression vector pET 22b found tobetherelativelypredominantisoform. –– AnimalBiotechnology –– maturation. Suppression function ofRIG1andMDA5 receptorsinbuffalo. provided crucialinsights intothe structureand pathway in buffalo fetal fibroblast cells. The study interferon regulatory factors (IRFs) mediated IFN-b andIFN-stimulated genes (ISGs)through I:C stimulation induced elevated expressions of Poly organs. immune to specific being without RLR geneswereubiquitousindifferenttissues compared to RIG1.Constitutive expressions of that MDA5 had a greater affinity towards dsRNA revealed calculation freeenergy Moreover, binding that hasbeenpreviouslyreportedinhuman. a similar,ifnotidentical,dsRNA binding pattern RIG1andMDA5follow that simulations suggested conserved among mammals. Molecular dynamics site residues of thereceptors areevolutionary that iscommoninmammals.Criticalbinding RIG1 andMDA5 maintainadomainarrangement antiviral response. Sequence analysis revealed that to understandthemechanismofreceptorinduced Buffalo RIG1andMDA5 genes wereinvestigated response. signals controllingTypeIinterferon(IFN-I) that recognizeviralRNAandmediatecellular intracellular innatepatternrecognitionreceptors RIG1 and MDA5 have emerged as important sensors suchasDAI, IFI16,AIM2 and DDX41. (NOD)-like receptors, and double strand DNA nucleotide-binding oligomerizationdomain (RIG-I)-like receptors(RIG1,MDA5&LGP2), (TLRs), cytosolicretinoic-acidinduciblegeneI including membraneassociatedToll-likereceptors numbers ofthesereceptors wereidentified, Agrowing peptides. andanti-microbial cytokines, of interferons, pro-inflammatory chemokines and recognition bythereceptorswillleadtoproduction or internal) Ligand (pathogen molecular patterns. the pathogenas well as from damage-associated recognition receptors (PRR)to detect ligands from germlineencodedpattern by damage. Itisinitiated including viral and bacterial infection, and tissue defense againstmicrobialpathogeninfection, The innateimmunesystemisthefirstlineof Innate ImmunityinIndianCattleandBuffalo breeding bulls. play crucialroleindeterminingthefertilityof hint thatepigeneticdefectsofspermDNAmay and embryonicdevelopment.Theobservations cell development, spermatogenesis, capacitation methylated geneswerefoundinvolvedingerm differential fertility status. Identified differentially be differentially methylated inbuffalo bulls of components and functions were identified to Differentially expressed proteins in MEC isolated immediate early,peakandlatestagesoflactation. to identify theproteinchangesinMEC during 2-D DIGEandmassspectrometrywereused producing ability of farm animals. In this study, milk the reflect (MEC) cells epithelial mammary alveoli whichsurroundsthelumen.Thesealveolar a branchingnetworkofductsthatendswith lactation: epithelial cells during different stages of Identification of protein signatures in mammary Human Health Improved and OtherBioactiveCompoundsfor Foods withPrebiotics,Probiotics,Micronutrients, Research onNutraceuticalsfromMilk,Functional induced signalingpathwaysandwhereasAkt, stage of lactation are associated with NF-κB stress lactation. The proteins up-regulated duringlate biological pathwaysandnetworksinvolvedin expressed proteinsweremappedtotheavailable catalytic andbindingactivity.Thedifferentially process, areassociatedinmetabolic proteins that majorityofthedifferentiallyexpressed showed analysis Bioinformatics identified. were and 22proteins inhighand low milk yielding cows stages lactation during identified were proteins were compared.Fortyonedifferentiallyexpressed from milk ofhighand low producingmilch cows The mammary gland is made upof and transport. The biological processes that they and transport.Thebiologicalprocessesthat activity, catalytic activity, ion channel activity molecular functionslikebinding,receptor proteins showed their involvement in different the of analysis ontology Gene identified. were expressedproteins uniquedifferentially Sixteen spectrometry. mass forMALDI-TOF/TOF was extracted protein spots were excised from gel andpeptide expressed differentially the proteins andthen Electrophoresis) wasperformedoftheisolated mitochondria isolationkit. Qproteome cellsby fromthese collected were andmitochondrialfractions Cytosolic for subcellularfractioncollection. in differentiatedcellsandtheseweretaken (as an indicator of differentiation) wereobserved prolactin after4daysofserumstarvation.Domes growth mediumwassupplementedwith5µg/ml subcellular fractioncollection.Fordifferentiation Proliferative cells of 70% confluency were taken for FBS. 10% and EGF ng/ml 10 hydrocortisone, ml supplemented with5µg/mlbovineinsulin,1µg/ grown in growth medium containing DMEM/F12 in ourlabwasused.Frozencellswerethawedand 25to30passages available BuMEC celllinebetween well as mitochondrial fractions. Forthispurpose differentiated BuMECs differentially expressedbetween proliferative and was designed to observe whether proteins get for productionofmilkcomponents. Experiment gland areinvolvedinmanymetabolicprocesses Differentiated BuMECsinlactatingmammary between ProliferativeandDifferentiatedBuMECs in Buffalo Mammary Epithelial Cells (BuMECs) Identification of Differentially Expressed Proteins through insulinhormonesignaling. associated withhighmilkproductionmediated PI3K andp38/MAPKsignalingpathwaysare in vitrobothcytosolic as DIGE (DifferenceGel –– AnimalBiotechnology ––

15 ndri annual report 2013-14 16 ndri annual report 2013-14 isoforms. Out of these BuPAG isoforms, one can BuPAG6, 7,8,9,13,15,16,18andonenew identified BuPAG isoforms are BuPAG2, BuPAG4, expressed duringtheearlystagepregnancy.The revealed that there are a total of 20 BuPAG isoforms correlated withtheirtimeofexpression.Thestudy of BuPAGswereanalyzed.Thesequences and sequenced.Existenceofvariousisoforms containing recombinantplasmidswerecloned coli (45 d, 75 d and 90d). The cDNA was cloned in E. cotyledons from the early stages of pregnancy was preparedfromtheisolatedRNAof12fetal cDNA pregnancy, various isoforms duringearly to gaininsightonthetranscriptabundanceof information aboutPAGsis veryless. Inorder is particularly of concern in buffalo where the diagnosis remainsuncertaininmanyaspects.It expression oftheseisoforms,itsuseinpregnancy and inadequateinformationaboutthetimeof the existenceof a large numberof PAG isoforms ofand earlyembryonicmortality.Butbecause has beenusedasamarker forpregnancydiagnosis released into maternal circulation, their expression Since, PAGsaremadefromfetalcotyledonsand Pregnancy inBuffalo Associated Glycoproteins(PAGs)duringEarly Pregnancy of Isoforms Various of Identification milk synthesis. folded and unfolded proteins synthesizedduring play theroleofproperfoldingpartially synthesizing themilkcomponents, chaperones under stressforprovidingtheenergyrequiredin cell sare mitochondria ofdifferentiatedepithelial during stress condition.As wehypothesized, were heat shock proteins (chaperones) expressed expressed proteins and interestingly 3of them mitochondrial fractionsshowed5differentially organization andtransportprocess.DIGEofthe process, metabolic process, cellular component and energygeneration,cellcycle,developmental are involvedinincludeprecursormetabolite –– AnimalBiotechnology –– Frequency of11differentisoforms PregnancyAssociated cloning vector.Atotalnumberof360clones Glycoproteins (PAGs) caused reducedrateoffertilization. inhibition ofoocytereceptorswithrec-Spaca3 Preliminary results suggested thatcompetitive zona andhelpinbindingofspermswithoccyte. that thereceptors for Spaca3mightbepresenton binding ofSpaca3withoocytes,whichsuggested in thezonaregion.Removalofcausedno Spaca3 could bind with buffalo oocytes specifically rec- wasshownthat clear.It isnot interaction howeverthemechanismofoocyte interactions; suggest thatSpaca3 might beinvolved in sperm- playing whenexpressedonsperms.Somereports Spaca3. ItisnotclearwhatroleSpaca3mightbe observed mutatedtoAlaandAsn,respectively,in residues Glu35and Asp53 inlysozyme were which might resultinlossof activity. Two catalytic suggested mutationofcatalyticresiduesinSpaca3 against not showlysozyme-likeantibacterialactivity sequence identitywithlysozyme.Spaca3could enzymes, however, the proteins share only 40-42% Spaca3 (128residues) belongs tolysozymetypec Associated 3(SPACA3)Protein Functional CharacterizationofSPermACrosome followed byBuPAG18. abundant isoformatallthestagesofpregnancy respectively. BuPAG 7 was observed to be themost 75%, and 78% be to found was PAG 6) (BuPAG homology withBovine(BoPAG16)andbubaline has beenreportedbefore.OnBLASTit’smaximum be considered as novel as no similarhomologue observed. Incontrolexperiments,the oocyteswereincubated spaca3 antibodiesfollowedbyincubationwithFITClabeled immunostaining. Oocyteswereincubatedwithhumananti- with anti-spaca3antibodies.Thehoechst 33342wasusedto directly withsecondaryantibodies withoutpre-incubation secondary antibodies.Theimmunostainingofintactzona since nostainingaroundoocyteswithzonaremovedwas (Green color)indicatesthebindingofSpaca3withzona, Localization ofrecLYZL3bindingsitesonoocytesby . Sequence analysis lysodeikticus.Sequenceanalysis Micrococcus counterstain thenucleus(Bluecolor) The in E.coli Protein (Fbp)andCollagenBinding(Cbp) Recombinant Expression ofFibronectin Binding milk electrical conductivity of normal, subclinical 6.79±0.34 and 7.17±0.43,respectively. Likewise, the in KaranFriesthevalues averaged 6.68±0.23, were 6.55±0.19,6.63±0.27 and6.93±0.37; The correspondingmeanvalues incaseofSahiwal 6.64±0.35, 6.78±0.41 and 7.09±0.29, respectively. mastitis andclinicalMurrahbuffaloeswere models. ThemilkpHofhealthy,subclinical Neural Network(ANN) and Fuzzy Logic based parameters were taken for formulation of Artificial Haptoglobin (Hp)values and the linearized Phase Proteins:SerumAmyloid-A(SAA)and Acute cows andbuffaloeswereusedtoquantify conductivity (EC) and pH from mastitis affected detection model,patternofchangeinmilkelectro- develop theANNandfuggylogicbasedmastitis yielders. Inthisstudyaimedto high particularly serious problem of all classes of dairy animals, of mastitisincowsand buffaloes: Mastitis is a Development ofneuro-fuzzysystemfordiagnosis Impact onLivestockProduction Management Practices, Climate Changeand its Systems UsingBetterHousingandFertility Development of State-of-the-art Dairy Production recombinant lactoferrin could bind and release systems, C-terminal lobeswereexpressedinyeastexpression Buffalo lactoferrin and its monoferric N- and (rec-Lf) inYeast Recombinant Expression of BuffaloLactoferrins vitro andinvivoconditions. to competitivelyexcludethepathogensunderin 16°C. Thefunctionallyactiveproteinswillbeused formwasachieved atexpression inthesoluble was studied at various culture conditions. Partial in BL21(DE3) E. coli PCR confirmation for the presence of the amplified and cloned in the pET22b(+) vector. After fbp of1.7kbpsizefrom concentration. Fibronectinbindingproteingene, temperature, IPTGconcentrationandsurfactant for solubleexpressionofCbpbyvaryingthe in the soluble fraction. Conditions were optimized expressed was partially Cbp studies showedthat Expression cloning. confirm to performed was cloned intopET22bexpressionvector.ColonyPCR gene was amplified from amplified was gene cbp Klurouymyces lactis and Pichia pastoris. The host cells, gene expression L L. acidophilusNCFM,was ivestock L. reuteri P gene fnbp gene roduction and it couldbedetrimentaltoanimalcellgrowth. at highconcentration yet is aantimicrobialagent, These results suggestedthatalthoughlactoferrin BuMECs. in κ-casein and β-casein of expression lactoferrinalsoreducedtheThe recombinant viability of BuMECs in a dose dependent manner. culture decreasedtheproliferationandcell the buffalomammaryepithelialcell(BuMECs) Exogenously addedrecombinantbuffaloLfto and biologicalactivityofrecombinantlactoferrin. inhibit papainsuggesting thenative-likestructure couldalsolactoferrin. Theexpressedlactoferrin iron almostinasimilarfashionasthenativemilk This experiment was designed tostudy the for detectionofestrusin KaranFriescows: Efficiency of wireless sensor device (pedometer) breeds. three model wasalsodevelopedfor allthe gave themaximumaccuracy. Thefuzzylogic cases. TheANNmodelofBayesianregularization Hp values being more in subclinical than in clinical of cows, however, only SAA showed such trend, subclinical and then to clinical mastitis state. In case to values fromhealthy increase inSAAandHp higher pHandECofmilk.Murrahshowedgradual 4 parity order and higher milk production had higher values in winter. Animals with more than pH andEC in summer whereas Sahiwal showed Murrah and Karan-Fries showed an increased milk stage oflactationwasprominentinallthebreeds. mS/cm, respectively.Theeffectofseasonsand cows, these were 5.68±0.23, 6.96±0.76 and 7.67±1.13 mS/cm and 7.94±0.91mS/cm and inKaranFries in Sahiwalcows were 5.55±0.19 mS/cm, 7.13±0.53 cm, respectively.Thecorrespondingmeanvalues averaged 4.64±0.35,6.42±0.72and7.54±1.02mS/ and clinicalmastitis affectedMurrahbuffaloes recombinant Lf(red),N-lobe(green)andC-lobe

Iron releasebyrecombinantintactLf,N-lobeandC-lobe and of Lf.CurvesrepresentintactnativeLf(blue), M anagement (orange) ofbuffaloLf

17 ndri annual report 2013-14 18 ndri annual report 2013-14 respectively). In second experiment, significantly experiment, second In respectively). 724.86±83.63 and2057.86±311.30 vs.934.99±30.57, periods (P<0.05) between600-1200hrs and1200-1800hrs displaced moresteps oestrous cycle.Cowsinheat stages (proestrus,metestrusanddiestrus)of other to compared (P<0.05) higher significantly active estrus averaged4699.25±768.51, which was it (934.99±30.57).Totalnumberofstepsduring cows inestrus phase(2057.86±311.30) and without in activitieswas foundduring1200-600pmin (P<0.001) increase significant Highly differences. with nonestrusphaserevealedsignificant of the activitiesbetweensimilarstagesinestrus and 245.515±35.07,respectively).Comparison and lowest during 0-600hrs period (1053.445±90.77 movement wasobservedbetween1200-1800hrs different periodsofthedaysandhighest between activity average in variation (P<0.001) significant into 6hourintervalsinexperiment–I.Therewas hourly activitywasrecorded and wassegregated procedures remainingsimilar.Theaverage was measured using pedometer, withallother the midcyclepostpartumanimalsandactivity on injection PGF2α by induced were 18) = (n recorded. InExperimentII,activitiesofanimals for physicalproperties.Heatintensitywasalso for progesteroneestimationandcervicalmucus the dayofheat,bloodsampleswerecollected by visual observation and rectal palpation. On normal estrusbehaviour,whichwasrecorded recorded bypedometerandcorrelatedwith of KaranFriescows(n=8)wasroutinely activity I, InExperiment wereconducted. experiments in KaranFriescows.Forthestudy,twoseparate the heatthroughpedometerbyhormonalassay confirm to as well as methods conventional the indigenously developedpedometerdeviceagainst efficiency of heat detection through application of –– Livestock Production and Management –– when not in heat (1549.41±270.10 vs. vis-a-vis whennotinheat(1549.41±270.10 vs. the farmersprovidedsuchservices aredelivered stakes forthepoorshepherds,could beadopted by financial immense with technologies, based ICT Though illiterate; this study revealed that modern special mineralsupplement duringmigration. for call findings these function, reproductive and Mn andZn.Mineralsbeingcriticalforproductive in Ca,Pand Cuand Fe;whereas theyhad access of deficient were sheep migratory The respectively. 6.02%, and 21.68% averaged migration en-route The mortality of young lambs and adult sheep migration during 0.92% to dropped drastically veterinary service while at home was 48.52% which others. Overallsatisfactionoffarmersregarding losses duetodiseases,theftofanimalsamong which include; lack of proper living amenities, the migration,farmersfacenumberofproblems (65%) occurred for a period of 6-9 months. During Haryana insearch of feedingand water resources towards flocks sheep of migration The 142. being together withagriculture;averageflocksize rearing sheep in 57.50% and rearing sheep in engaged were farmers 38.0% Nearly Hindi. and but could communicate in Marwari, Rajasthani middle age group,weremostlyilliterate(85.00%) to belonged farmers the of (65.00%) majority were selected.Resultsofthestudyrevealedthat migratory route;100samplesfromeachcategory the and sheepthrough mineral statusinsoil,plant to Rohtakwererandomly selected. For analysis of 20 farmers’throughthemigratoryroutefromPali respondents from Marwar region of Rajasthan and the nomadic flocks in Marwar region. A total of 120 facedby animal continuumtoaddresstheproblems the incidenceofdisease together withsoil-plant- information onsheepmigrationpatternandstudy This study was planned to collect real time delivering managementinputsforsuchlocations. communication forimprovedsurveillanceand using GPSholdspromise in dataacquisitionand communication network.Trackinganimals problems ontherouteofmigrationduetopoor tradition and the shepherds face a number of Migration ofsheepinRajasthanisalongstanding migratory sheepofaridzoneRajasthan: Studies onsoil-plant-animalcontinuumin predicted. oestrus so that insemination time can be accurately while calculatingtheexacttimeofinitiation algorithm needstobedevelopedforautomation estrus detection indairy animals, however, proper It wasconcludedthatpedometercanbehelpfulfor 152.26±8.90, P<0.05)vis-a-visotherstagesofcycle. vs. (252.61±11.10 observed was injection, PGF2α higher activityduring estrus, 67.5hrs after yield ofthecows(19.78vs. 17.55kg/cow/d),it milk housing managementnotonly improvedthe proofing. Itwasrevealed thattheimproved bedding, fence-linefeeding andfly/mosquito width ofthecovered area(20vs. 10 feet), sand by increasingtheheightof roof(15vs. 10feet), traditional loosehousing duringwinterseason werehoused underimprovedvs.lactation comparison, crossbreddairycowsintheirearly Inthis system: housing loose amodel under Performance and behaviour of dairy animals on body weightandothernongeneticparameters. can beusedtopredictthelameness of cows based lameness of cows with more than 80% accuracy and implies thatANNmodels were abletopredictthe This strategy. partition data 80:20 at (83.19%) rate accuracy classification highest with MLP to in detecting theleghealthstatus incomparison optimization algorithm, gave the best performance neural architectwithLeven-burgMarquardt(LM) that to predict the health status of leg in cows; RBF different optimization algorithms. Itwasfound and 80:20),numberofnodesinhiddenlayers and validationdatasets(60:40,70:30 for training carried outusingvaryingdatapartitionstrategies was ofnetwork (NNs).Simulation networks (MLP) and Radial Basis Function (RBF) neural used for thedevelopment of Multilayer Perception milk yieldasinputvariables.Thesevariableswere and stage of pregnancy, stage of lactation and daily individuallegs,parity,status weight distributedto non-genetic variablesincluding; percent of body status oflameness as targetvariable,and other 589 KaranFriescowswasrecordedfortheexisting to NAIP project.Datapertaining developed inthe balance the fourquadrantsWSNbasedweighing status ofleghealthfromthedatacollected study, analgorithmwasdevelopedforpredicting effective treatment of hoof and leg ailments. In this of bovinelamenessenablesthefarmertoprovide of lamenessinthesecrossbreds.Earlydetection folds, simultaneouslyithasalsoincreasedtherisk no doubt,hasincreasedmilkproductionseveral crossbreds werealsoreported.Thecrossbreeding; animals. Higherincidencesinexoticandtheir milk productionandreproductivestatusofthe breed, parity, sex, age, upon depending 38.24% lameness isreportedtovarybetween8.82and and reproductiveproblems.Theincidenceof clinical conditions ofdairycows aftermastitis is thethirdmostcostliestandwidelyprevalent weight using Artificial Neural Network: Detection oflamenesscowbasedonbody communication. smart phonewithaudio-visual modeof through Lameness Lameness group, asagainsttwotimes bullparadingas observation ofcardinalsigns ofestrusin treatment 1800h), heatexpectancychart alongwithvisual parading inadayat0600h, 1000h,1400hand was carriedoutusingateaser bull(4-times and accuracy in Murrahbuffaloes. Estrus detection efficiency detection estrus the improving in tools study theeffectivenessofcertainmanagement conception rateinMurrahbuffaloheifersandto on cooling effect ofshort-term the to findout of ways.Thisstudywas,therefore,conducted adversely affectstheherdfertilityinanumber in buffaloes, particularly duringsummer months, Murrah buffaloes:Inadequatedetectionofestrus accuracy, efficiencyandconceptionratein Management tools forimprovingheat detection advancing theageatpubertybyabout3-4weeks. beginning 18 months of age was effective in administration ofGnRH@4µg/heifer/month However, the not improvetheirageatmaturity. concentrate alonetogrowingSahiwalheifersdid 2 groups.Thus, thesupplementation ofadditional heifers (G1A2andG2A2)ascompared totheother significantly (P<0.05) lower inGnRH administered was G2A2, respectivelyandtheageatpuberty and 600.83±15.33daysforG1A1,G1A2,G2A1 averaged 630.54±8.66,610.62±7.5, 625.45±20.66 puberty at age The significantly. differ not did as theDMI/100kgBWoftreatmentgroups period aswell treatment the during gain weight starting 18months of age. The overall average daily administered 4 µg GnRH at monthly interval the heifersinG1A2andG2A2were each sothat so astohave4treatmentgroupsof6heifers G2A1 andG2A2)after8fortnightsofexperiment subdivided into 2 groups each (G1A1, G1A2, over G1.TheheifersinG1andG2werefurther TDN) 70% and CP % (20 extra concentrate g standards whereasheifersinG2received500 G2. The heifers in G1 were fed as per NRC2001 were dividedintotwoequalgroupsG1and In study 24Sahiwal heifers, aged 12 months, growth andageatpubertyinSahiwalheifers: Effect ofmanagementandseasonbirthon crossbred cowsduringwinterseason. milk productionandensuredbetterwelfareofthe improved housing management ensured higher (8.57 vs.8.29h/cow/d). It was inferred thatthe (7.27 vs.7.07h/cow/day)andruminationtime intake (15.04vs.14.84kg/cow/d),eatingtime of the housing management type on the DM resting atanypointoftime.Therewasnoaffect cows the of proportion the as measured 81.87%) cow/d) andtheoverallcowcomfort(88.75vs. also improved the resting time (13:26 vs. 12.52h/ –– Livestock Production and Management ––

19 ndri annual report 2013-14 20 ndri annual report 2013-14 affected affected KF cows. However, RFM did not influence milk yieldandtotal wasfoundinRFM days 305 in decrease (P<0.05%) Significant RFM. for factors risk significant were (OR=1.75) season of calf (OR=1.95) and calving during summer gestation length(OR=12.52), lowerbirthweight dead calf (OR=4.39),pluriparity(OR=1.67),shorter buffaloes, dystocia(OR=4.74),abortion(OR=19.21), (OR=6.39) were significant risk factors. In Murrah of calf (OR=1.61)and shorter gestation length (OR=3.98), dead calf (OR=3.72), lower birth weight abortion (OR=3.91), In Sahiwalcows,dystocia (OR=1.47), shortergestation length(OR=4.31). (OR=3.96), deadcalf(OR=4.29),pluriparity for RFMinKFweredystocia (OR=4.29), abortion factor risk significant and respectively, 13.08% and 15.59% 26.37%, were buffaloes Murrah and RFM incidencefrom2001to2012inKF,Sahiwal and quantifytheiroddsratio(OR).Theoverall model was used to identifyriskfactors for RFM were analyzed.Amultivariablelogisticregression Sahiwal and1604lactationsinMurrahbuffaloes number of 2547 lactations in KF,1609 lactations in years (2001to2012)datawereanalyzed.Atotal performance indairy cattle and buffaloes, 12 the impact of RFM onproductive and reproductive identify theriskfactorsforRFMandtoanalyze performance indairycattleandbuffaloes:To RFM onproductionandreproduction impact of of FetalMembrane(RFM)andstudiedthe Retention for factors risk the of Identification compared toatypicalfernpattern(20.00%). as (54.90%) rate conception higher (P>0.01) mucus having typical fern pattern had significantly in Murrahbuffaloheifers.Buffaloeswithcervical humid seasonandimprovedtheconceptionrate body temperature of buffalo heifers during hot and hours before & after AI) significantly reduced the Further, shorttermcoolingaroundbreeding(3 efficiency inMurrahbuffaloessignificantly. visual observation improvedtheheatdetection bull parading,heatexpectancychartandthe involving;4timesIt wasinferredthatpackage treatment group as compared to control (79.17%). following rectal examination was found inthe detection accuracy, albeit not statistically, (85.46%) estrus higher Moreover, (40.70%). group control to compared as group treatment in (63.65%) efficiency was found significantly (P<0.05) higher progesterone onthedayofestrus.Estrusdetection rectal examinationandbyestimation ofplasma estrus detectionaccuracywasobservedbyper using average estrus interval in buffaloes while efficiency wascalculatedbyTimm’sequation The estrus detection carried outincontrolgroup. –– Livestock Production and Management –– increased from 7 day 2postpartumandconcentrationsofBHBA till increased significantly from 14 from significantly increased cows affected with RFM, concentrations of NEFA compared tothosethatcalvednormally.InKF RFM had that cows in higher significantly were the concentrations of NEFA, BHBA and BUN the fetalmembraneswithin12h.However, lower whencomparedtothosethatexpelled significantly were TP and glucose cholesterol, of (SW) cows.IncowsthatdevelopedRFM,thelevels their relationship withRFMinKFand Sahiwal study to 1,2postpartum) andonday day ofcalving non-RFM cows(on30,14,7,5,3,1daypre-partum, butaric acid (BHBA) were estimatedinRFMand non-esterified fatty acid (NEFA) and beta-hydroxy protein (TP), blood urea nitrogen(BUN), glucose, blood concentrationsoftotalcholesterol, cattle:Peripheral dairy andcrossbred indigenous Metabolic andbiochemicalindicatorsforRFMin (p<0.05) theincidence of metritis intheseanimals. and calving interval significantly but, it increased service, serviceperiod,servicesperconception first to calving from interval length, lactation the those that expelled the fetalmembranes normally. RFMcomparedto developed (p<0.05) incowsthat expression was7.7–10.0and 3.4–4.4foldslower mRNA andGPR43 lower, andGPR41 3-4 times was tissue extract in theutero-placental butyrate The concentrationof using GasChromatography. SCFAs wereestimatedintheutero-placentaltissues that developed RFM(n=12).Concentrations of membranes normally(n=12)andfromthecows tissues obtained from the cows that expelledfetal were studiedusingqRT-PCRintheutero-placental acid (SCFA)receptorsandadhesionmolecules fatty chain short cytokines, inflammatory pro of idea about thepathogenesisofRFM. Expression an give to expected is molecules inflammatory of expression the studying process, inflammatory membranes isan elusive. Sinceexpulsionoffetal predictive markers for theconditionstillremains pathogenesis forRFMandidentificationof fetal membranes:Inspiteofextensiveresearch, tissues altered incowsdevelopingretentionof utero-placental in cytokines pro-inflammatory Expression ofshortchainfattyacidreceptorsand identifying the cows atthe risk of developing RFM. in pre-partum periodwouldhelp BHBA during be inferredthatmonitoringthelevelsofNEFAand due calvingwas highly relatedtotheRFM.Itmay ofNEFAandBHBAduring30days concentrations partum tillday2postpartuminRFMcows. The pre- 5 day between significantly increased level calving. Similarly,inSWcows, NEFA andBHBA th daypre-partumtill2 th daypre-partum nd dayafter or spermatogeniccells from live animalwithout need tostandardizeamethodisolatetheSertoli castration method. Thus, there has beenapressing maleiscompromised in ofthe ability reproductive slaughter housesample based method, the future unknown in details oftheanimalremainslargely Sertoli orspermatogeniccells.Whereas,the castration wasusedtoisolateandculturethe obtained eitherfrom slaughter house or through testis bulls:Tilldate, bull calvesandbreeding spermatogenic andSertolicellsfromlive Method forextraction and culture of SCFA receptorsisassociatedwithRFMincows. and molecules adhesion cytokines, inflammatory that inadequateoralteredexpressionofpro- for normalexpulsionoffetalmembranesand concluded that inflammatory response is essential incidence ofRFMincows.Itwas in reducingthe these molecules priortoparturitionmight help of fetal membranes, modulating thelevels of and SCFAsreceptorswasobservedwithexpulsion molecules adhesion cytokines, pro-inflammatory clear associationofbutyratelevels,expression compared to thosethatdevelopedRFM.Sincea normally expelledthefetalmembranes normally and PECAM were higher(p<0.05)inthecows that ICAM TNFα, IL-8, IL-6, IL-1, of expression The described purified proteins of spermatozoa of two two of spermatozoa of proteins purified described 2D-DIGE analysis was performed byusing above To investigate the differentially expressed proteins, categories were subjected to proteomic analysis. Spermatozoa obtainedfrom bullsofboththe fertile. low or high as classified were bulls and bull per inseminations artificial 100 of minimum a on based 50-75%) between inheritance Holstein with crossbred bulls(HolsteinFriesianxThaprakar, bulls: spermatozoa fromhighandlowfertilecrossbred Identified differentially expressed proteins in Conception rates (CR) were estimated for Conception rates(CR)wereestimatedfor Growth andmultiplicationofSertoli(A)spermatogeniccells(B)during invitroculture(after5passages) A and 91%,respectivelyafter10passages. cellswere93 andspermatogenic ofSertoli viability exclusion blue the Trypan test. It was the found that by cellswasestimated cultured ofthe The viability passages with a doubling time between 5-7 days. the cell.The sertoli cells were sub-culturedfor10 to maintainthegrowthandactualperformance of done was subculture 70-80%, reached confluence growth wascheckedundermicroscope.Whencell cells, media was changed every third day and the as described above.To ensure proper growthofthe was done at a conc. of 100µl/ 3ml culture medium the sertolicellpellet, plating pellet. Aftergetting was centrifuged at 1000 rpm for 2min to get the cell the lectinthoroughly.Then,sertolicellsuspension trypsin, 0.2% EDTA) and washed twice to remove plate were detached by using trypsin EDTA (0.25% bound tolectin.Thesertolicellsthelectin with spermatogeniccellsandthesertoliwere cells wereseparated.Thesupernatantwasenriched On day1,thespermatogeniccells and the sertoli day ofplatingwasconsidered as 0dayofculture. Sertoli andspermatogeniccellsstandardized. The bulls andtheculturemethodologyofextracted standardized toextracttesticularcellsfromlive was method biopsy needleaspiration percutaneous affecting its futurereproductive performance. The for DIGE analysis. It was observed that 21 protein for DIGEanalysis. It was observed that 21 protein on alltheinternalstandardimages wereconsidered standard gelimages.Onlymatched spotsdetected of 700±15proteinspotsweredetected intheinternal in totalcorrespondingtothe twogels.Anaverage generated foreachgel.Siximageswere samples (HF,LFandinternalstandard)were software. Threeimagescorrespondingtothethree Quant andresultswereanalyzedbyusingDeCyder by using CyDye. Gels were scanned in Image extracted from HFand LF spermatozoa werelabelled groupsHFand LF,respectively.Proteins distinct –– Livestock Production and Management –– B

21 ndri annual report 2013-14 22 ndri annual report 2013-14 Sertoli and spermatogenic cells from peri-pubertal Sertoli andspermatogeniccellsfromperi-pubertal and exoticbullcalves:Theproteomicanalysisof in spermatogeniccellsfromindigenous,crossbred Identification of differentially expressed proteins using haematoxylin/eosinstaintostudythehisto- obtained by unilateral castration were stained individual testicularcells.Testiculartissuesections out tostudythemorphologyandbiometryof Testicular fineneedle aspiration was carried 10m) fromeachbreedwereutilizedforthestudy. bulls werestudied.Fourperi-pubertal(age Fries (KF-crossbred) and Tharparkar (indigenous) cells inHolsteinFriesian(HF-purebred),Karan seminiferous tubules,spermatogeniccellsandSertoli bull calves: The histomorphometric characteristics of cells amongpurebred,crossbredandindigenous cell numbersandarrangementsofspermatogenic Testicular histology indicates differences in Sertoli over expressedinLFspermatozoa. 12 proteinsoverexpressedin HF and3proteinswere MALDI-TOF/MS analysis were identified in which selected spots,15differentiallyexpressedproteinsby spots weredifferentiallyexpressed.Amongallthe –– Livestock Production and Management –– Testicular sectionindicatingthecompactnessofseminiferoustubule,intensityLeydigcellsandstatusspermatogenesisin DIGE imagesoftesticularcellproteins withdifferentiallyexpressedspotsasanalysedbyDeCyderTMsoftware. [A]–HFxKF spermatogenic cells;[B]–KFxTP cells;[C]-HFxKFSertolicells peri-pubertal bullsA-HolsteinFriesian,B-KaranFries,C-Tharparkar A of total214proteinspotsinspermatogeniccells (DIGE) and image scanning identified the presence calves usingdifferenceinGelElectrophoresis bull Holstein Friesian,KaranFriesandTharparkar different agesinthesebreeds. at architecture testicular the study to and findings breeds. Further studies are in progress to confirm the which mightexplainfertilitydifferencesamongthese in testicularcomponentsamongthebreedsstudied, exist variations that indicate findings preliminary These HF andTharparkarcomparedtoKFbulls. in higher (p<0.05) significantly was cells Sertoli of bulls comparedtoTharparkarbulls.Theproportion of Sertoli cells was significantly (p<0.05) higher in HF Tharparkar bullsfollowedbyKFandHF.Thearea bulls. TheintensityofLeydigcellswashigherin was highinTharparkarfollowedbyKFandHF of compactness,basedonqualitativeevaluation, by KFandTharparkarbulls.However,thedegree the seminiferous tubule was high inHF,followed cells. Itwasobservedthatthediameterandareaof used tostudythepopulationofSertoliandLeydig architecture of the testes. GATA4 antibody was also B C in bullsof3corresponding groups was20.4,18.0 ejaculation first at age average The kg. 276.2 and groups being 20.3, 20.0 and 20.6cm; and 260.7, 261.3 respective the of mount first at weights body the circumferenceand sizeofscrotal corresponding the treatmentgroupsC,I& II,respectivelywith mounting at15.3,14.2and14.3monthsofagein starteddifferent parametersrevealedthatbulls ejaculation weremonitored.Averagevaluesof at age and mounting first at age circumference, respectively, over control. Body weight,scrotal % energy, 20 and additional 10% fed and IIIwere as pertheNRC(2001)standardandgroupsII of 6animalseach.Controlwerefedon the study. These calves were managed in 3 groups months and ~190 kgbodyweight,weretakenfor Eighteen bullcalves,attheaverageageof~9 bulls. buffalo Murrah in ejaculation first at age increased feed energy levels on bodyweightand study wasconductedtoinvestigatetheeffectof maturity and semen quality in buffalo bulls: Effect offeedenergylevelsonpuberty, sexual found tobeoverexpressedincrossbredbulls. Friesian bulls,whereasSpeedyProteinAwas to beoverexpressed in theSertolicellsofHolstein Speedy protein-Arespectively.γ-Actinwasfound identified as Gamma Actin (γ-Actin) and RINGO/ identified as albumin, whereas spots 9 and 12 were selected spots of Sertolicells, spots 10 and 11 were a two fold increase in expression. Among the 4 of crossbred bullscompared to Tharparkarwith was overexpressed inthespermatogenic cells Ethanolamine Binding Protein (PEBP), which Phosphatidyl as identified was 8 spot the and asalbumin, 7wereidentified The spots1to TP xcrossbred and SertolicellsofHFxcrossbred. from spermatogeniccellsofHFxcrossbredand belonged tothethreeexperimentalsamples,i.e. MS. Outofthese12proteins,4proteinseach MALDI-TOF/ through identification further for after silverstaining,12proteinspotswereselected andvisibility expression ofdifferential magnitude of differentially expressed proteins, based on the panel fromthe analysis, image DIGE After groups. ranged from 3.1 to 36.9 folds between twobreed spots expression ofprotein bulls. Thedifferential under expressedinHFcomparedtocrossbred Friesian bulls,whereas26proteinspotswere the spermatogenic cells isolated from Holstein this, 14 proteins were found over expressed in thegroupswereselected. Basedonbetween at least two fold differences in expression level having spots study, Forfurther groups. the in both majority ofthespotsweredifferentiallyexpressed from HFandcrossbredbulls.Amongthese, A observed tobethemajorvoicecueswhichcould mean intensity andformants (especially F1)were features thecallduration,totalenergy,meanpitch, acoustic features ofvoice.Amongtheallacoustic concentrations werenegativelycorrelatedwithall noise toharmonicratioandformantF1.LH correlated withminimumamplitude, negatively pitch andmeanintensityofvoice,whileitwas duration, maximumamplitude,totalenergy,mean concentration was positively correlated with call and proestrus periods respectively. Estradiol diestrus while F1and F2 werehigherinmetestrus during higher significantly were F5 and F4 F3, formants increasedlinearlyfromF1toF5inwhich voice also followed similar trend. Values of various by estrus, metestrus and diestrus. Mean intensity of significantly higher mean pitch and was followed and diestrus. The voice in the Proestrus stage had higher inproestrus,followedbyestrus,metestrus and diestrus. Total energy was found significantly (P<0.05) inproestrusfollowedbyestrus,metestrus ratio. Maximum amplitude was significantly higher trend wasobservedincaseofnoisetoharmonic Same diestrus. from significantly differed stages proestrus, estrus,andmetestrusstagesbutall3 among significantly differ not did voice the of diestrus (P<0.05)phases. Minimum amplitude during estrusandproestrusthanmetestrus call duration of buffaloes was significantly longer use inestrusdetection.Resultsrevealedthatthe of estrous cycle with thepurpose of exploring its acoustic featuresofbuffaloesduringvariousstages study was conducted to assess the variations in the cues for efficient management of dairy animals: Acoustic assessmentofindexicalvocalization kg, respectively. 23.9, 23.4 and 25.0 cm; and 350.3, 340.2 and 364.1 were; groups treatment 3 the of ejaculation first of scrotal circumference and the body weightsat 19.3 months.Thecorrespondingvaluesforsize Percentage ofbreedablecrossbredfemale intotalbreedable population –– Livestock Production and Management –– A

23 ndri annual report 2013-14 24 ndri annual report 2013-14 HSFs, HSPsandMC1RusingRealTime-PCR and Karan-Friescattlewasevaluatedbyanalyzing Sahiwal Tharparkar, of fibroblast dermal the on dermal fibroblast of cattle: genes in HSFs, HSPsandMC1R Expression of analyzing theexpressionofheatshockproteins dermal fibroblast ofbuffalo was evaluated by onthe stress ofheat Theeffect buffalo: of fibroblast dermal the on stress heat of Effect Agriculture (NICRA) National InitiativeonClimateResilient Milk yield during treatment and aftertreatment in suitable drug for treatment ofclinical endometritis. fertility parametersrevealedOTCwasmore gentamicin, OTC and povidone iodine through vivo evaluation(n=8;i.u.routefor3days)of as 5.47µg/mland0.78µg/ml,respectively.In gentamicin andOTCagainstE.coliwasfound minimum inhibitoryconcentration(µg/ml)of and chloramphenicolweremostsensitive.The ampicilin with sulbactum,oxytetracycline(OTC) against was carriedout.Antimicrobialsensitivityassay uterine swabofcowswithclinicalendometritis of of 12.70% coliforms, of 22% of and buffaloes: Isolation cattle indairy uterineinfections postpartum Bacterial and pharmacological concerns of phases ofestrouscycle. unfold thevariationinvoicesignalsofdifferent and 22% of 22% and Sp. Staphylococcus revealed that gentamicin, ceftriaxone, E. colirevealedthatgentamicin,ceftriaxone, , 19% of 19% E. coli, 23.53% sp. Streptococcus The effect of heat stress The effectofheatstress Bacillus sp.from D airy C attle P factors forendometritis. along withhigherlevelsAPPsareimportantrisk lower concentration ofchemoattractant (IL-8) concentrations of pro-inflammatory cytokines, of negativeenergybalancehigherorverylow presence in that, inferred was It TNF-α. & IL-6 IL-1β, by mediated mostly are APPs Effectors poor predictorduringearly(7days)postpartum. SAA, while AGPwas of clinicalendometritisthan predictor revealed plasmaHpcouldbethebetter leptin) concentrationduringtransitionperiod metabolites/hormones (NEFA, BHBA, IGF-1 and cytokines IL-1β, IL-6, TNF-α and IL-8) and energy 1 acidglycoprotein:AGP),pro-inflammatory ofAPPs(Hp,SAA and Alphacows. Estimation cows andinsignificanteffectonPItreated inflammatory processesinantibiotictreated diminishing the suggested TNF-α) and (IL-1β Hp andserumamyloidA:SAA)cytokines antimicrobial therapythroughAPPs (haptaglobin: all thegroupsremainedunchanged.Evaluationof down regulatedwhereasHSP-70.1 results showed that HSFfactors (HSF-1, 2,4)was and 44 metalloproteins (MMPs) by exposing at25,37,40 (HSPs), apoptoticgenes(caspases)andmatrix the increaseintemperaturefrom37to44 apoptotic genesand MMPs were increased with compared tocontrol were upregulated at different temperature as hysiology ° C. Theexpressionofheatshockproteins, . and ° C. HSP-90 showed the insulation deficiency and when the when and deficiency insulation the showed breakage fromthelegsataround15–18°Cwhich 27°C. Insulationisotherm detected the insulation the thresholdlimitoftemperaturewasselected spot havingthemaximumtemperaturewhen the differenceinisotherms.Eyeswerehot Image analysisusingFLIRtoolsoftwarerevealed buffalo was carried out duringdifferentseasons. seasons: Thermal different profiling of cattle and during buffalo and cattle of profiling Thermal confidence. coagulation andsignallingpathwayswithhigh proteins divulgedthepresenceofcomplement, whereas thepathwayanalysis localization oftheidentifiedserumproteins characterization, biologicalfunction and cellular the identified proteins revealed the molecular of profiling proteome serum the for data first the is This serum. buffalo the in confidence high with identified were proteins 55 total In MS/MS. LC identified throughMALDITOF/TOFandESI fold changewerepickedthroughspotpickerand differentially expressed spots showing significant thoroughput 2DIGEproteomicsapproach. heat stressandrecoveryperiodapplyinghigh of profile proteome serum conducted todecipherthedifferentiallyexpressed heat stressinbuffalo(Bubalusbubalis):The study Identification of genes and proteins in relation to morphological, physiological, haematological (MC1R andASIP)their relationshipwith out theexpressionofcoat colorrelatedgenes (TP) andKaranFries(KF)cattle: In order to find Tharparkar in traits unique of Identification Approach: Percentage ofmilchpopulationintotallivestock Bubalus bubalis.Thegeneontologyanalysisof Vulnerability=AdaptiveCapacity–(Sensitivity+Exposure). Bubalus bubalisduring of the identified the of The

energy leakagethatdepictsvasoconstriction. insulation levelfalls below apresetvalueof the component arebeingdeveloped. of individual indicators of theadaptivecapacity downloaded, tabulatedandcleanedthemaps for eachofthevulnerabilitycomponentwas mapping, allIndiadistrictleveldataof600districts vulnerability: For Vulnerability assessment in summerthanwinterseason. physiological responses were significantly higher higher duringsummerthanwinter.Similarly, wassignificantly ofbuffalo and dorsalsite abdomen the in flow blood that showed Results were measured during summer and winter season. different physiological parameters and blood flow physiological reactionsofMurrahbuffaloes:The Seasonal variations in blood flow and than winterseasoninboth thebreeds.The summer during higher 0.01) < (P significantly and winterseason. Physiological responses were summer onTPandKFheifersduring carried out and biochemicalparameters, presentstudywas Percentage ofbreedablecrossbredfemaleintotal population –– DairyCattlePhysiology ––

25 ndri annual report 2013-14 26 ndri annual report 2013-14 of metabolichormonesi.e.tri-iodothyronine(T (P <0.05) higher inKFthanTP heifers. cortisol during summer season was significantly TP heifers.The magnitude of increase inplasma than KF in higher 0.01) < (P significantly were magnitude of increase in physiological responses –– DairyCattlePhysiology –– Sahiwal calves. Supplementation of chromium treatment group compared to control group of in status immunity higher showed TNF-α) and IL-6 IL-2, (IL-1β, parameters Immunological higher incontrol compared to treatment group. levels ofstresshormonecortisolwerefoundtobe calves duringsummerandwinterseasons.The group comparedtocontrolofSahiwal CAT, GPxandGR)werefoundintreatment enzymes (SOD, The lowervaluesofantioxidant observed in drymatterintakeamongtwogroups. group, whereasnosignificantdifferencewas higher intreatmentgroupcomparedtocontrol Body weight continuously. gain was significantly during winterand summer seasons for45days intake/day dry matter chromium propionate/kg Treatment group of animals were fed @ 0.5mg (with chromiumpropionatesupplementation). (without chromiumpropionate)andtreatment i.e.control groups two (6each)into equally on 12Sahiwalcalves, which werefurtherdivided propionate: Amelioration ofthermalstressusingchromium importance ofthesegenestoadaptation. other heat stress related parameters indicated the changes intheirlevelofexpressionalongwith between thetwo breeds of cattle. Further,the (MCIR, PMEL)duringboththeseasonsand in theexpression level of skin color related genes difference significant the showed clearly study plasma cortisollevel.Theresultsofthepresent heat stressasitwasnegativelycorrelatedwith showed negative correlation (P < 0.01) with also genes responses. Theexpressionofthese showed negativecorrelationwithphysiological reverse thecoatcolor.Theexpressiongenes than KFcattle,whereas,thehaircolorwas winter season.ThecoatcolorofTPwasdarker (P <0.01)higherinTPthanKFespeciallyduring significantly was expression of magnitude The than summerseasoninbothTPandKFheifers. PMEL) expressedmore(P<0.01)duringwinter responsible for skin pigmentation (MC1R and compared tosummerinboththebreeds. significantly (P<0.01)higherduringwinter enzyme forskinpigmentation(tyrosinase)was both thebreeds.However,levelsofessential higher duringwinterthansummerseasonin and thyroxine(T The presentstudywasundertaken 4 ) were significantly (P < 0.01) < (P significantly were ) The levels Genes 3 ) these factorsmaycauseprogrammed cellcycle aggravated bythermalstress. Upregulationof indicated highercellloss whichwasfurther ratio (apoptosisindicator)on thedayofcalving and BAK(proapoptoticgenes), BAX/BCL-2 the breeds.Thus,upregulationofCaspase3 respect to15dayspreandpostcalvinginboth (P<0.0001) loweronthedayofcalving with condition. Also thelevelsweresignificantly summer ascomparedtothethermoneutral were significantly(P<0.0001)lowerduring and postcalving.PlasmalevelofIGF-1levels the dayofcalvingwithrespectto15dayspre calving. Plasmalevelofleptinwasloweron of calving with respect to 15 days pre and post significantly (P=0.004)decreasedontheday between theperiods. The plasma levelofIgG change significant no was there but conditions, during summerascomparedtothermoneutral found tobesignificantly(P<0.0001)higher both seasons.PlasmalevelsofHSP-27were condition andinKaranFriescows(P<0.05) partum in Sahiwal (P<0.0001) in thermoneutral post and pre day 15 on lower significantly was summer inalltheperiods. The expression ofBAK upregulation ofBAKinSWandKFduring (P<0.0001) significant was There cows. KF in was downregulationofFASinallperiodsonly the breeds.Whilecomparingtwoseasons,there 15 daypreandpostpartuminsummerboth all periods.FASmRNAexpressionincreasedon of P-53 during summer in both the breeds and in breeds duringsummer.Therewasupregulation were lower on 15 day prepartum in both the compared withthermoneutralcondition.Ratios breeds, inallperiodsduringthesummerwhen BAX/BCL-2 ratios were also higher in both the both the breeds at different levels of significance. compared withthermoneutralconditionsin during summerwashigherinallperiodsas day ofcalving.Alsothemagnitudeexpression and thermoneutral condition as compared to 0 on 15dayspreandpostpartumduringsummer and KF cows decreased significantly (P<0.0001) period. TheCaspase-3mRNAexpressioninSW the cowswereathigherstressduringcalving and transitionperiodinSWKFcows.Also stress thermal of impact significant was There status. the negativeimpactonbodygrowthand immune thermal stress ameliorative measure for mitigating mg/kgDM/day couldbeusedasoneofthemajor supplementation ofchromium [email protected] ofSahiwalcalves. Thus,and immunestatus propionate improved DMI, body weightgain significantly more (P<0.05) in the supplemented gained morethan500g/day. Butthegainwas not supplementedwithfermented yeastculture control groupcalves(male/female) whichwere hormone. Underthepresent management,the the increaseinplasmaconcentrationof the plasma IGF I hormone and increased with less bodyweightexhibitedconcentrationof supplementation: Murrahbuffalocalveswith buffaloes withfermentedyeastculture inMurrah andpuberty growth augmenting for Evaluation ofplasmaIGFIandHaptoglobin micronutrient supplementation. neutrophils (isolatedatcalving)variedwith in vitroconditions,theresponseofstressed neutrophils was observedatcalving.Under Significantly higher (P<0.01) apoptosis of blood once theymigratedtothemammarytissue. neutrophils changedsignificantly(P<0.01) at calving.Shape,viabilityandPAofblood genes: TLR-2,TLR-4,TNF-alpha,IL-1andIL-8 lower expression was observed in neutrophilic collegenase enzyme levels. Significantly (P<0.01) cows. Buffalo neutrophils had more elastase and also improvedreproductiveperformanceofKF Micronutrients group. in VitAsupplemented supplemented cowsandimprovedmilkyield Supplementation decreasedmilkSCCinVitE postcalving, whereas,Cuincreasedthem. plasma cortisollevelsatcalvingand30days reduced (P<0.05) significantly Zn and E Vit of blood BandTlymphocytes.Supplementation had nobeneficialeffectonthefunctionof E, Cuandcombinationgroup. Vit Asupplementedbuffaloesfollowedby phagocytic activity(PA)ofbloodneutrophils the in observed was effect beneficial Maximum modulated thefunctionofbloodneutrophils. E,CuandZn Vit ofVit.A, supplementation peripartum dairyanimalstodisease.However, activity and expression that predisposes the was observedwhichdecreasedtheneutrophilic levelsaroundcalvingplasma micronutrients and buffaloesperipartum:Adeclineinthe in vivoandvitroofhighproducingcows immunocompetence ofneutrophilicfunction of modulation basisfor Molecular respect to15dayspreandpostcalving. and IgGdecreasedonthedayofcalvingwith thermoneutral conditions.Plasmalevelofleptin to summerascompared decreasedduring IGF-1 condition. PlasmalevelofHSP-27increasedand during summersascomparedtothemoneutral arrest, cellularsenescenceorapoptosisofPBMC Supplementation treated spermatozoagroups deliveredresults groups. without treatmentinboth high andlowfertile min. whencompared withthespermatozoa with motilityandviability foraperiodof120 spermatozoa In vitrosupplementation ofthesefactorsto group whencomparedwithlowfertilegroup. significantly greater(P<0.05)inhighfertile sperms withplasmalemmaintegritywerealso percentage of motile and viable spermatozoa, The parameterswithrespecttospermatozoa, significantly more (P<0.05) in high fertile group. that levelofthebloodplasmagrowthfactorswas studied inMurrahbuffalobulls.Itwasobserved IGF I, NGF growth factors and fertility status was The relationshipbetweenblood,seminalplasma hepatocyte parameters. culture, reversedtheeffectofhyperthermiaon addition of mannan oligosaccharide to the cell when compared with the mean±SE activity at 37 (P<0.01) significantly increased had peroxidase superoxide dismutase,catalaseandglutathione conditions, the activity of antioxidant enzymes in vitro.Itwasobservedthatunderhyperthermic and 72h time intervals under controlled conditions greater (P<0.01) only at 42 is a stress marker, was observed to be significantly secretion with significantdecrease(P<0.01)inalbumin with respecttofunctionalmarkersofhepatocytes at differenttimeintervals.Resultswereopposite conditionsat 40 and 42 hyperthermic to weresubjected they and then secretion dehydrogenase activity,albuminandurea the functionality ofhepatocytes likelactate parameters for assessing to cellnumber,viability, Hepatocyte culturewasoptimizedwithrespect 22 monthsofage. case ofmalebuffaloes3/6attainedpuberty,at (1/6) attainedpubertyat24monthsofage.In months of age.Whereas unsupplemented group female buffaloes(5/6)attainedpubertyat21±1 more insupplementedgroup(P<0.05)andthe significantlywas I IGF plasma of concentration months ofagetillpuberty,itwasobservedthat of calves @ 24g/animal/day from fourteen was furtherconductedforthesamegroup weight. Whenthestudywithsupplementation concentration ofplasmaIGFIandgaininbody 12g/animal/day significantlyincreasedthe supplement likefermentedyeastculture@ group withADG>800g/day.Moreover, In vitrofertilizationexperiments with . The secretion of TGF β, which β, TGF of secretion The in vitro. in vitrooveraperiodof72hat37 increased thepercentageofsperms o C for a period of 24, 48 o C at 72h interval. On –– DairyCattlePhysiology –– o o C C

27 ndri annual report 2013-14 28 ndri annual report 2013-14 in augmentationofmilksecretion andearlier buffaloes. Prilledfatfeeding wasquiteeffective NDF intreatmentgroupover thecontrolgroup improved (P<0.001)digestibility ofEEand producing buffaloes.Prilled fatsupplementation low producing buffaloes as compared tohigh in less significantly were NFE and NDF ADF, Digestibility coefficients of DM, OM, CP, (P<0.001). significantly levels NEFA reduced low producers. Prilled fat supplementation (P<0.001) inhighproducingbuffaloesthanthe (P<0.05). PlasmaNEFAconcentrationwasmore significantly group fed fat prilled in increased between lowandhighproducinggroup,but levels weresignificantly (P<0.05)different more (P<0.001)bodyweights.Plasmaglucose and DMI in increase significant to due group, the treatmentgroupincomparisontocontrol in 10.96% @ yield milk increased (P<0.001) Supplementation ofprilledfatsignificantly partum and@150g/dtillday120oflactation. Murrah buffaloes@75g/duptoday45post In another experiment, prilled fat was fed to the reproductive performanceofcows. to augment milk secretion and improvement of prilled fatwascosteffectiveandcouldbeused the improvedconceptionrate.Thefeedingof postpartum cyclicity,service periodshort and the cowswasimprovedbyearlyinitiationof decreased. The reproductive performance of cholesterol levels;howeverHDLconcentration affected plasmahormones,VLDL,BHBAand effect onthedigestibilityofnutrientsnor prilled fatneithercausedanydetrimental decline inbodyweight.Thefeedingof restricting reservesby ofbody mobilization effective in physiological adjustment of that prillfatfeedinginearlylactationwas concentration increased(P<0.05)indicating decreased (P<0.05)andplasmatriglycerides cows. However,plasmaNEFAconcentration cholesterol in comparison to control group without affecting lactose, protein, milk in milk yield (P<0.05) and milk fat (P<0.05) of prilled fat resulted in a significant increase 75g/day foraperiodof90days.Thefeeding The experimentalcowswerefedprilledfat@ and were given 10 days adaptation period. selected atanaverage20dayspostpartum and buffaloes: Lactating crossbred cows were performance oflowandhighproducingcows Metabolic adaptationandearlylactation compared withuntreatedgroup. with greaternumberofcleavedembryoswhen –– DairyCattlePhysiology –– of milkproduction@10kg/day. effective intermsofinput–output ratioatthelevel guduchi supplementation. The technology is cost effect. The milk yield improved by >12% through immune systemintermsof immune-stimulatory reportedly healthyduetotheimprovementof the animalssupplementedwithguduchiwere technology atfasterrate.Theresults revealed that ofmixture willalsofurtherhelpinadoption The supplementation of guduchi in the concentrate powdered forminopenmarketwasaconstraint. ofGuduchiin thatavailability was observed costeffectiveness.It andits from themarket of thetechnologyandavailabilityguduchi a survey was conducted regarding awareness doorstep, farmer’s at field the in technology of on guduchi supplementation and evaluation of 4weeks.Forobtainingfeedbackfarmers stimulatory effectinmiceonfeedingforaperiod supplemented buffaloesexhibitedimmuno- indicated thatmilkobtainedfromguduchi to controlmilkfedgroupwasfound.Theresults of micefedonguduchimilkascompared group treated in LPI high Significantly 1.38±0.14. and 0.98±0.09 control andtreatedmicewasfoundtobe (P<0.05). LymphocyteProliferationIndexin respectively registeringasignificantincrease treatment groupswas 52.93±4.34 and62.61±4.57, The percentphagocyticactivityincontroland in groupofmicefedonguduchimilk. weeks of feeding was significantly higher (P<0.05) respectively. Theincreaseinweightofmiceafter4 1.95 and3.63gincontroltreatmentgroups, and 28.50±2.59.Thenetgaininbodyweightwas values intreatmentgroupofmicewere24.87±2.06 and 27.65±3.45,respectivelywhiletherespective weight (g)ofcontrolgroupmicewas25.70±1.94 final and initial average The milk. with replaced grams of5.0grams)thesyntheticdietwas (2.5 50% The weeks. four for was experiment of guduchi supplementedbuffaloes.Theduration treatment groupwasfedonmilkobtainedfrom guduchi supplementation and buffaloes without freeze driednormalmilkobtainedfromcontrol of 8miceeach.Thecontrolgroupwasfedon buffaloes, 16miceweredividedintotwogroups dried milkobtainedfromguduchisupplemented effectoffreeze immunomodulatory the study level: field at performance reproduction and peripartum todairyanimalsonproduction Influence ofguduchisupplementation and bodyweightdeclineinbuffaloesalso. intake ofdrymatter adjustment physiological To to eachtreatment,lowestCH significantly (P<0.05)onadditionofnitrate Total N,NH a similartendencywasobservedinIVOMD. nitrate (T promising effect interms of reduction ofCH that additionofnitratetothe ruminantdiethas nitrate supplementation.This studyindicated TVFA (mM/dl)increasedsignificantlywith groups whencomparedwithT (P<0.05) for theeach nitratesupplemented basis. CH and fiber level on level fiber and Effect ofvarious levelsof bromochlormethane Addressing ClimateChangeScenario Resources Utilization and Use ofNon-ConventionalFeed Nutrient Efficient Strategies, Feeding Improved Animals through Dairy Raising Productivityof can be used at 3% level of DMI in the ruminantthe in DMI of level 3% at used be can Thus, itcanbeconcluded that calciumnitrate with increased ammonia concentration in rumen. was observedinT and T T 40:60) ratioofwheatstrawandconcentrate(T with threedifferentsubstrate(60:40,50:50and production and rumen fermentation parameters conducted toassesstheinvitrodigestibility,gas was Thepresentstudy emission: methane Nitrate as prophylactic option tomitigate adversely. affecting otherrumen fermentation parameters seems tobeeffectiveinmethanereductionwithout the basis ofpresent study 4mM dose ofBCM combinations. On BCM additioninalltreatment (A: P)ratiowasreducedby Acetate: propionate percentage ofpropionateandbutyrateincreased. source. Acetateconcentrationwasreducedwhile, asaroughage andberseem straw wheat containing increase inmost ofthecases with totalmixeddiet was notadverselyaffectedbyBCM but foundto (IVDMD) indifferenttreatmentcombinations dependent manner. and methane production (>98% of control) in a dose gas total reduced BCM μM). 12 and 8 4, (0, BCM using 3 levels of fibre (HF, MF, LF) and 4 levels of measured bypreparing 12 treatment combinations production and fermentation characteristics was bromochloromethane (BCM) onmethane and fermentation pattern: The effect of 2 and T 6 (21.64). IVDMD was significantly lower significantly was IVDMD (21.64). 3 ), each supplemented with 3 % calcium 4 4 , T production (% of total gas) decreased 3 5 -N, NPN,TCA-pptN(mg/dl)and andT 4 in vitro dry matter digestibility In vitro dry matter digestibility 6 (18.25 followedbyT ), g/100gsubstrateonDM methaneproduction 4 production (%) production 1 , T D 2 airy andT 5 C (19.78 3 , and attle 1 4 , with 16S PCR analyses (Fig.1) and administered to to and administered (Fig.1) 16SPCR analyses with isolated, screenedandmolecularly characterized at 200 μg/ml linoleic acid for 1hr incubation) was fibrisolvens bacteria (140.77μg/ml CLA production fatty acids:AhighCLAproducing Butyrivibrio pathways resulted inmodificationofrumenmetabolic Butyrivibrio fibrisolvensadminstrationhas methanogens diversityincattle. three ordersprovidingbetterinsightsofrumen to 16S rRNA gene, mcrA gene OTUs clustered in compared straw, wheat containing diet fibrous present inKaranFriescrossbred cattle fedonhigh Methanobrevibacter phylotypewaspredominantly with Methanosarcinales. These results revealed that Methanomicrobiales andremainingonegrouped order Methanobacteriales, tworesemble with with clustered fifteen library, gene mcrA from stadtmanae. Outof eighteenOTUs identified whereas oneOTUresemblewithMethanosphaera of which aligned with Methanobrevibacter spp., clustered with order Methanobacteriales, twelve thirteen OTUsorphylotypesfrom16SrRNAlibrary All primers. specific using libraries gene subunit) α reductase coenzyme-M (methyl mcrA and cattle wasdeterminedbyconstructing16SrRNA of rumen methanogens in crossbred Karan Fries fibre based diet: diversity ofmethanogens in cattlefedonhigh The 16SrRNAandmcrAgenebasedcomparative Developments Molecular Aspectsof Dairy NutritionandNewer production inlowRFIgroupofSahiwalcalves. methane reduced and efficiency feeding higher in low RFI than high RFI group. The results showed as g/kg DMI was also significantly (P<0.05) lower (58.71) ascompared to highRFIgroup(65.62)and significantly (P<0.05)lowerinlowRFIgroup production whenexpressedasg/daywas than mediumandhighRFIanimals.Methane RFI low in higher (P<0.05) significantly also was high RFIgroups.Nitrogenbalance and retention (P<0.05) higherinlowRFIthanmediumand all nutrients except NDF were significantly for coefficients digestibility The RFI. high and were divided into threegroupsoflow,medium emission insahiwalcalves:MaleSahiwalcalves on methane residual feedintake(RFI) Effect of reduce methaneproduction. ration ascheapfermentablenitrogensourceto N utrition via modulationoftheabsorbed In the present study, the diversity In the present study, the diversity –– DairyCattleNutrition

29 ndri annual report 2013-14 30 ndri annual report 2013-14 2.63 kg/100kg BW, and digestibility coefficient (%) kg manurefermentation.Dry matterintakewas from 10and20 emission was1.4and9.31g/day protein supplements(7.78). The averagemethane (10.78), rangeforages(9.46), treeleaves(8.69)and supplements (12.40), non-legumefodders energy followed byagro-industrialby-product(14.66), TOMD) wasobservedinlegumefodders(19.29) emission(g/kg methane Highest products. fractions were higher in agro-industrial by products and range forages, whereas indigestible fractions wereabundantinagro-industrialby- and legumefodders.Intermediatelydegradable and proteinwerehigherinsupplements the readilydegradablefractionsofcarbohydrate manure fermentation.Thedatarevealedthat Methane releasewasalsoestimatedfromequine molecular technique usinguniversalprimers. by caecum horse in identified were methanotrophs and rumenliquor.Acetogens,methanogens vitro degradation andmethaneemissionunderin were evaluatedasperCNCPSandfordrymatter from equine hindguts: Common equines feeds Methane evaluationandmethaneproduction in milk in 18% and 10.56% by and meat in 25% and 12.2% by increased significantly was SFA milk oflactatinggoats.UFAandratioto in 40% by increased was but meat in unchanged respectively. TheconcentrationofMUFAremained milk and meat in 33.56% and 15.6% by increased and milk,respectively;whereastotalPUFAwas meat in 24.3% and 10.5% by decreased was SFA Total andlactatinggoats,respectively. in kids 50% and 30% by increased was milk and meat 6.34% in lactating goats, respectively. Total CLA in and 5% by and kids; in 43% and 33% by was observed. MUFA and UFA increased (P<0.05) goats) lactating in 5% and kids in (4.04% PUFA in increase significant a whereas goats; in lactating 10% and kids in 6.24% by SFA in decrease a milk. Fatty acid analysis through GC revealed and also in ruminant derived products, meat and inrumenbiohydrogenation alterations the check to female goats kids (malegoats)aswelllactating –– DairyCattleNutrition conditionsusingequinefaecal inoculums Real TimePCRGraph,StandardCurveandMeltPeaksofR.flavefaciens -0.20 and+0.18kg/d.TheDM intakewaslower respectively withcorresponding RFIvaluesof 124.23 and115.85154.89144.20,to be recorded were animals the of BW final and initial The groups. RFI efficient) (less high and efficient) Sahiwal calvesweredividedintolow(more male residualfeedintake:Eighteen different mineral metabolism in sahiwal calves with Nutrient utilization,blood parametersand in comparisontoruminants. methane emission from equine feeds and manure the presentstudiesmaybeareasonforlower acetogens andmethanotrophsasidentifiedin and methanogens,respectively.Thepresenceof and balaforFTHFS,typeI,IImethanotrophs 1571285 withclonesnameseq1,kundu,radha Bankit number1570827,1571273,1571282,and deposited intheGeneBankdatabase underthe The nucleotidesequencewereobtainedand wereamplifiedusingPCR.and methanogens) (acetogens) and 16S rRNA genes (methanothrophs partial nuceleotidesequenceencodingftfsgenes 66.77, 53.48,46.94,44.03and47.56respectively.The of DM,CP,EE,NDF,ADFandNFCwere59.34, meat andmilk. mechanism responsible forCLA accumulation in indirectly theregulatory acids, thus,altering fatty metabolic pathwaysviamodulationoftheabsorbed rumen of modification in resulted administration Therefore, (34.1%). T3 and (20.1%) T2 groups administered the both in (P<0.05) higher significantly were fluid rumen fibrisolvens aswelltheirrelativeabundancein in blood.ThepopulationdensityofButyrivibrio resulted inanincrease inantioxidantactivity Further, theeffectofButyrivibrioadministration observed in different treatments in lactating goats. and composition (fat,protein,SNFandlactose) was respectively. No significant differences in milk yield 62.54% in rumen fluid of kids and lactating goats, and 50% by increased significantly was activity 13.25% in milk. However, Linoleic acid isomerase and meat in 12.5% by decreased was acid fatty Butyrivibrio administeredgroups.Ratioof n-6:n-3 Butyrivibrio fibrisolvens

FD-1 used as feed supplement for flavefaciens strain It was found to be Ruminococcus biochemical propertiesandmolecularproperties. characterized on the basis of its morphology, degrading bacterialisolatewasselectedand fibre potent most the and measured was activity enzyme Their diet. fibre high on kept buffaloes strains were isolated from the rumen liquor of lactating murrahbuffaloes:Fibrolyticbacterial and itscomposition,nutrientutilizationin on body weight changes, milk production Effect ofisolatesfiberdegradingbacteria and Additives Productivity EnhancementthroughSupplements levels were in the normal range in both the groups. low RFI compared to high RFI group. However, the of Ca,PandZninplasmawashigher(P<0.05) were similarin both the groups. The concentration excretion andretentionofCa,P,Fe,Cu,ZnMn absorption, urinary low RFIgroup.Theapparent the groups.Thefeedcostperkggainwaslowerin IGF-1 andGHconcentrationweresimilar in both in highRFIgroupofSahiwalcalves.Theplasma IU/L) and AST (80.33 vs 100.57 IU/L)werehigher group. Also,theactivitiesofALT(25.85vs.35.72 g/dl) werefound to behigher (P<0.05)inhighRFI 22.22 mg/dl) and total plasma protein (7.34 vs.8.24 (15.45 vs. vs.1.78mg/dl) andbloodureanitrogen groups. The overall mean values of creatinine (1.27 Blood glucosevaluesweresimilar inboththe absorbed) was higher (P<0.05) in low RFI group. the groups. N retention (% of N intake and % of N RFI groups,respectivelywhichweresimilarinboth 54.62) and ADF (44.01 and 42.96%) for low and high and 55.69),EE (64.43 and63.18),NDF(55.84 and 60.61), CP(63.78and 63.28), totalCHO(55.95 nutrients were:DM(59.68and59.14),OM(61.38 groups. The digestibility coefficients (%) of various ADG valuesweresimilar (539 and498g)inboth kg/100 kgBW in low and high RFI groups. Mean across the fortnightswas2.45±0.10and2.77±0.12 to highRFIgroup.The overall DM consumption (3.26±0.08 vs.3.43±0.08kg/dinlowRFIcompared matter intake was significant (P <0.05) between <0.05) (P significant was intake matter on milkcomposition. Thedifferenceinmeandry supplemented treatedgroup. Therewas no effect and dailymilkyieldwasincreased inliveculture and controlgroupsalthough thelivebodyweight the treated changes anddailymilkyield between difference wasobservedinthemeanbodyweight significant No month. one for continuously days flavefaciens strain FD-1;onalternate culture ofR. and300mlliveautoclaved diets experimental and controlgroupsofsixeachwerefedwith Twelve lactating buffaloesdivided into treatment

in vivo

trial. in T yield (kg/d)wassignificantly(P<0.05)higher ALT and AST. Results revealedthatoverallmilk total plasmaImmunoglobulin,glucose, their respectivecalves were collected to estimate 70 days. Blood samples from all the animals and supplemented. Theexperimentaltriallastedfor higher inT significantly was (%) solid total whereas groups whereas inT daily in the concentrate mixture. Experimental mixture. inthe concentrate daily group werefedwithbasaldiet plus27gRPCmixed mixture (50%) as per NRC (2001). Calves in treatment concentrate compounded and (40%) fodder maize ration consisting of wheat straw (10%), chaffed green weight (n=6animalspergroup) andwerefedbasal andbody ofage basis onthe groups two divided into (KaranFries)calveswererandomlycrossbred metabolic profile in crossbred calves: on growthperformance,nutrientutilizationand Effect of rumen protected choline supplementation increased milkyieldandbetterimmunity. cow/d) andcholine(60g/cow/d)resultedin protected methionine(6g/cow/d),lysine(60g/ It can beconcluded thatsupplementation of groups whereasincalves, the values were similar. treatment in higher significantly found were dl) Total plasma immunoglobulin levels incows (mg/ decreased onlyincalvesoftreatmentgroups. gets calves, whereasALT(IU/l)activity and their AST (IU/l) activity was decreased both incows similar inalltheexperimental groups.Theplasma but therestof milk components werestatistically was also significantly (P<0.05) higher in T in higher (P<0.05) significantly also was comparison to C(14.74±2.16,). The milk fatcontent methionine and 60g rumenprotectedlysineinT rumenprotected 6g with were supplemented group werefedsame basal dietexceptthatthey treatment in Animals (2001). NRC per as (40%) mixture concentrate compounded and (40%) fodder maize green chaffed (20%), straw wheat animals were fed basal ration consisting of of MPPA (n= 6 animals per group). All the were dividedintothreegroupsonthebasis (Karan fries) lactatingcows (0days inmilk) lactating crossbredcows:Eighteen composition andbloodmetabolicprofilein methionine, lysine and choline onmilk yield, Effect ofsupplementingrumenprotected not significant. that ofthecontrolgroupalthougheffectwas over 19.20% and 9.66 by group treated in higher digestibility of NDF and ADF was found to be kg/day) duringtheexperimentalperiod.The control (11.11 kg/day)and treated groups(11.77 1 (18.00±1.63,)andT 2 grouponlyascomparedtocontrol, 2 , 60grumenprotectedcholinewas 2 (18.27±1.96)groupsin –– DairyCattleNutrition Twelve 1 and T 1 2 ,

31 ndri annual report 2013-14 32 ndri annual report 2013-14 4 intake ofdigestibleDM, OM, CP,EE, NDFand Fries) lactatingcows(averaging11.56DIM;2 cows duringsummer:Eighteencrossbred(Karan utilization andbalancesinlactatingcrossbred Effect ofsupplementationniacinonnutrient g/d) intherationofcalvesisbeneficial. revealed thatdietarysupplementation ofRPC(27 10.10±0.06). Thus,theresultsofpresentstudies 10.47±0.07) ascomparedtocontrol(50.50±0.30and (P<0.01) increase intreatment group (52.33±0.45and significant a exhibited also (mg/dl) VLDL and dl) 101.81; 20.28and 21.15).The plasma triglyceride (mg/ 95.65; 18.48and20.15)ascomparedtocontrol(17.59; (16.91; group (P<0.01)intreatment significantly NEFA (µmol/litre),ALTandAST(IU/L)decreased two groups (P>0.05).However,BUN (mg/dl), plasma did not reveal any significant difference between the andtotalplasmaimmunoglobulin ratio A:G globulin, density lipoproteins(LDL),totalprotein,albumin, cholesterol, highdensitylipoprotein(HDL),low supplementation of choline (P>0.05). Plasma glucose, by influenced not were balance nitrogen and ADF kg). ThedigestibilityofDM,OM,CP,EE,NDF, (0.17kg) group by 6.3% as compared to control (0.16 treatment in higher (P<0.05) significantly also was efficiency feed average, an On kg). (8.52 control to higher intreatmentgroup(9.23kg)ascompared (P<0.01) significantly also was gain weight body Similarly, (0.57kg). control to compared as 8.78% increase inADGtreatmentgroup(0.62kg)by (P<0.01) significant a exhibiting groups treatment (P>0.05). Thebodyweight increased numerically in supplementation onDMI,CPintakeandTDN RPC of effect significant no was there that revealed and TDN intake were recorded fortnightly. Results intake CP efficiency, utilization feed gain, weight (DMI), bodyweight,averagedailygain(ADG), feeding lastedfor90daysinwhichdrymatterintake –– DairyCattleNutrition of drymatter(kg/100kgB.W. andg/kgW did not differ (P>0.05) in the three groups. The intake the animalswerein very severe stress. Body weight stress duringmorning,whereas during afternoon revealed thatanimalswerein moderateandsevere (at 2:30pm)andTHIwascomputed.Theresults recorded dailyinmorning(at7:30am)andafternoon relative humidityand dry-wetbulbtemperaturewere The maximumtemperature,minimum 600 (T basal dietexceptthatthey were supplemented with (2001). Animalsintreatmentgroupwerefedsame compounded concentrate mixture (40%) as per NRC and (40%) fodder maize green chaffed (20%), straw group) andwerefedbasalrationconsistingofwheat basis ofaveragemilkproduction(n=6animalsper th lactation) were divided into three groups on the lactation)weredividedintothreegroupsonthe 1 )

and

800 mg/kgDMI(T 2 )

niacin for 120 days. niacin for120days. .75 ) and ) and nd to to T DMI (kg/d) was significantly (P<0.05) higher in T in higher (P<0.05) significantly was (kg/d) DMI ADF weresimilarinallthegroups(P>0.05).But treatment groups(12020.01and14121.76inT in (P<0.01) higher significantly was (mg/d) balance not differamongthethree groups. However,niacin The nitrogen,calciumandphosphorousbalancedid not influenced (P>0.05) by supplementation of niacin. Digestibility ofDM,OM,CP,EE,NDFandADFwas A study Astudy insahiwalcalves: andgrowth utilization concentrate mixtureonfeed intake,nutrient maize inthe culled potatoesinreplacement of Effect of inclusion of different levels of Utilisation ofUnconventional Feedstuffs partum reproductivestatusofcrossbredcows. andpost- healthstatus choline improvedmetabolic with rumenprotectedmethioninepluslysineand concluded that supplementing pre-partumdiets overall conceptionratewassimilar.It animals incholinesupplementedcowswhereas days openaftercalvingwasapparentlylowerfor of andnumber conception per inseminations artificial of number cyclicity, of commencement animals. Among thereproductive parameters, and growth hormones were similar among all Weekly mean plasma concentrations of prolactin higher (P<0.05)incholinesupplementedgroup. the dayofcalving,vitaminElevels in plasma was also higher(P<0.05)insupplementedgroups.On very lowdensitylipoproteinsconcentrationwas -15 d.Phosphatidyl choline, triglycerides and decreased (P<0.05)insupplementedgroupsat of animals.Non-esterifiedfattyacidslevel urea nitrogendidnotdifferamongthreegroups metabolites viz. glucose, cholesterol and blood concentration formostoftheaminoacids.Blood cows. Plasma amino acid profile revealed similar (P<0.01) in protected amino acid supplemented methionine andlysine,respectivelywerehigher of MP) of (% flows duodenal whereas lactation, metabolizable protein (MP) and net energy for as crude protein, rumen undegradable protein on body condition score, nutrientintakes such litres) 30daysprepartum,therewasnoeffect effective choline)tocrossbredcows(MPPA4500 and 54gofrumenprotectedcholine(10 protected lysine(RPL, 4.42 gof effective lysine) g ofeffectivemethionine) plus 20gof rumen of 5grumenprotectedmethionine(RPM,1.98 vitamins: Itwas observed that supplementation supplementing limitingaminoacids and Productivity enhancementofruminantsby 800 mg/kgDMIhasnoeffectonnutrient utilization. concluded thatsupplementationofniacinat600and 2 , respectively) than control (4715.44). It can be , respectively)thancontrol(4715.44).Itcanbe 1 and and 1 .

T in treatmentsT found to be 525.00, 477.33, and 503.02, 526.33 g all the4groups.Theaveragedailygainswere and ADF)nitrogenretentionweresimilar in digestibility ofnutrients(DM,CP,OM,EE, NDF day) was4.14±0.40,3.83±0.44 and4.04±0.39in and ‘C’(65.74±1.73).Thedry matterintake(kg/ in ‘T-2’(56.54±1.25)followed by‘T-1’(59.04±1.41) IVOMD (67.92±1.10) and‘T-2’(65.05±0.93)group.The ‘T-1’ by followed (71.41±1.26) ‘C’ in (P≤0.05) in groups T culled potatoes@0,25,50and100%onDMbasis (2001) for60days.Maizegrainwasreplacedby body weightandagefedonrationsasperNRC into fourgroupsof5animals each basedontheir calves. Twenty Sahiwalmalecalvesweredivided nutrient utilizationand growth inSahiwal male of maize intheconcentratemixture onfeed intake, different levelsofculledpotatoesasareplacement of inclusion of effect out find to conducted was group. IVCPD ‘C’ (67.03±0.85)andlowest in‘T-2’(55.03±0.91) degradability (IVDMD of DM, OM and CP. The in vitro undertaken to determine the rumen degradability degradability studyofdifferentdietswasalso collected atmonthlyinterval.Aninvitrorumen was recordedfortnightlyandbloodsamples conducted for a period of 105 days. Body weight extracted ingroup‘T-2’.Theexperimentswere ‘T-1’ anddecorticatedcottonseedcakesolvent was replacedbycottonseedcakeexpellerin mixture contained 15% of soybean meal deoiled) 10 partsofsoybeanmeal(controlconcentrate fed samedietasthatoffcontrol‘C’except day. Animalsin‘T-1’and‘T-2’groupswerealso 50:50) alongwith10kgoatsgreenfodder/animal/ fed samebasalration(concentrate:wheatstraw animals ineachgroup.All theanimalswere were dividedrandomlyintothreegroupsofsix age (8-9months)andbodyweight(114-115kg), Eighteen KFcrossbredfemalecalvesofsimilar calves: crossbred of performance growth on Effect offeedingdecorticatedcottonseedcake nutrient utilizationinSahiwalmalecalves. and growth feed intake, affecting maize without 33% having mixture concentrate the in basis DM grain could be replaced by potatoes up to 75% on indicated similar rateofgrowth.Therefore,maize in treatmentsT averaged 391.51,376.47,389.62and373.98g/day and 2.8kg/dayintherespectivegroups.CPintake respectively. DM intake (% of BW) was 2.6, 2.9, 2.9 1 , T 2 , T 3 R and T (%) was significantly higher (P≤0.05) in 1 , T 2 4 , T R was 2.90, 3.08, 3.14 and 3.12 kg/d, 1 1 was significantly lower (P≤0.05) lower significantly was , T , T 3 and T 2 , T 2 , T 3 andT 3 andT R 4 ) (%) significantly higher , respectively. The DMI in 4 , respectivelywhich 4 , respectively.The rumen dry matter the dietofdairyanimals. N production of teosinte. The treatment combination ha toobtainhighergreenfodderandquality 193.37 kg/haandforPhosphorus was 58.19kg/ optimum doserecommendedforNitrogenwas use of 160 kg Nand 60 kg P the maximumgreenfodderproductionwith (0,30,60 kgP ha) inmainplotsandthreelevelsofphosphorus N/ kg 160 120, 80, 40, (0, nitrogen of levels five The projectwasconductedinsplitplotdesignwith phosphorus application ( Efficiency ofnutrientutilizationinteosinte meal deoiled. soybean 15% having mixture concentrate a in decorticated cottonseedcakesolventextracted of soybeanmealby cottonseed cakeexpelleror fries calves may be reduced by replacing 10 parts cost forperkgbodyweightgaininfemalekaran and 56.78in‘C’,‘T-1’‘T-2’respectively.The for perkgbodyweightgain(Rs)was73.65,69.38 cost The groups. three all in significantly differ ratioandhaemoglobin notalbumin: globulin blood glucose, total protein, albumin, globulin, than ‘C’ (14.77±0.61) and ‘T-1’ (14.67±0.58) while significantly higher (P≤0.05) in ‘T-2’ (18.08±0.64) nitrogen balance. Blood urea nitrogen was (28.78±1.14), allanimalswerefoundinpositive in ‘T-2’(37.30±5.61)and‘C’(30.95±2.61)than‘T-1’ groups. Nitrogen balance was significantly higher NDF and NFE not differ significantly in all three g/day). DigestibilityofDM,OM,CP,CF,ADF, group ‘C’ (634±61 g/day) and group ‘T-1’ (583±37 higher (P≤0.05) in group ‘T-2’ (750±51g/day) than significantly be to observed were gains daily average The significantly. differ not did which and 2.71±0.57in‘C’,‘T-1’‘T-2’respectively, intake (kg/100kgBW)was2.50±0.42,2.35±0.45 ‘C’, ‘T-1’and‘T-2’respectively.Thedrymatter and 60kgP teosinte can bemet from theapplicationof160kgN (473.13 q/ha)green fodder yieldin single cut from (4) replicationsduring2012and2013.Thehighest cowpea sownon25 period ofOctober-November. Cultivationof fodder duringlean of Augustcanprovidegreen of bajraandmaizesowninlastweek Cultivation sown duringpostmonsoonseason Comparative performanceofdifferentfodders farmers forcultivationofteosintefodder. Zea mexicanaL.)asaffectedbynitrogenand 160 P 30 kg/ha was recorded as advantageous to the kg/hawasrecordedasadvantageoustothe 2 O 2 5 O /ha. Therefore, the farmer can obtain /ha. Therefore,thefarmercanobtain 5 /ha) insub-plots consisted four th Augustcanhelptobalance 2 O 5 /ha. The economic –– DairyCattleNutrition

33 ndri annual report 2013-14 34 ndri annual report 2013-14 determined by qRT-PCR. Reducedexpressionof determined by junction proteins)was (Occludin andZO-1tight two importantgenesrelated tointestinalintegrity permeability could beobserved. Expression of probiotic fermentedmilkon intestinalintegrity/ A positiveeffectofdietarysupplementation and measurement of fluorescenes in blood plasma. determined by using 4000 Da fluorescent-dextran as permeability intestinal in increase significant a in resulted feeding diet w/w) (35%, fat High Swiss Albinomice fed onhighfatdiet (HFD). and relatedgeneexpressionwasevaluatedusing reference tointestinalintegrity/permeability L. caseiinmodulationofintestinalfunctionwith Effect ofmilkfermentedwithaprobioticstrain Fed Conditions Milk on Intestinal Health Under High FatDiet Effect ofProbioticLactobacilluscaseiFermented to haveadditiveeffects. (commercial preparationsused)wasnotobserved incorporation ofherbalingredientpowders in resistingtheprogression ofobesity.Dietary ) (especiallyL.rhamnosus ofprobiotic potential Adiponectin and leptin genes indicated the HFD for 12 weeks. Analysis of expression of feeding on significantly increase to found were group. Fastingblood glucose and insulin levels as was observedinprobiotic(L.rhamnosusaswell triglycerides in decrease significant A VLDL-C. A similartendencywasobservedforTC,LDL-C, triglyceride levelascompared to controlgroup. the in increase significant a to led feeding diet fat could beobservedwithL.rhamnosusfeeding.High to control group and maximum positive effect animals were observedtobelargerascompared to HFD+SMfedgroup.AdipocytesofHFD reduced epididymalfataccumulationascompared plantarum supplementation ofprobiotic (L.rhamnosus/ L. 12 weeks. Dietary (HFD) +Skimmilk(SM),after diet fat as comparedtoanimalsfedhigh weight significant effect in terms of reduced gain in body fermented milktohighfatdietexhibiteda groups, dietarysupplementationofL.rhamnosus fed C57BL/6 mice. Among the different treatment of obesitywasevaluatedinhighfatdiet(HFD) (Aloe veraandGymnemasylvestre rhamnosus andL.plantarum)herbalingredients Effect of dietarysupplementationprobiotics(L. Influence theDietInducedAdiposity Probiotic Fermented Milk and Herbal Ingredients L. plantarum)fed groups relativetoHFD+SM ) as well as herbal ingredients resulted in ) onprogression A nimal B iochemistry

decreased inlymphocytes.Interleukinsanalysis whereas stimulationindexandCD28expression C reactiveprotein(CRP)levelsincreasedinplasma Macrophage Chemotactic Protein-1 (MCP-1) and a decrease inTLR-2 and TLR-4 expression. and phagocytosiswhilemacrophagesrecorded decrease inactivitiesofrespiratoryburstenzymes intestine. Neutrophilsregisteredaremarkable in plasmaandhumoralimmuneresponse macrophages, splenocytes, inflamm-aging markers peripheral blood lymphocytes, peritoneal various immuneparametersinvolvingneutrophils, of 4, 8,12and 16 months old were analyzedfor dysregulation withprogressiveaginginmice. to delineatethenatureofimmune in anattempt analysis of different immune cells was performed immunosenescence. Therefore,aconcurrent to theobservedambiguity inunderstanding comparisons inimmunefunctionscontributes with ageareknown.The lack of multipoint age regarding natureofdysfunctionimmunesystem Several contradictionsandinconsistentreports Immune System Age-associated Profile of Dysfunctions in Murine fat dietfedconditions. in high functionality fermented milkonintestinal containing casei L. ofprobiotic supplementation present study suggest a positive effect of dietary found to be statistically significant. The findingsof exhibited positiveeffects,butthesewerenot level. Though, skim milk supplementation also milk fedgroup,however,nottoasignificant of ZO-1wasalsoobservedinprobioticfermented fermented milk. A similar trend of up-regulation as aresultofdietarysupplementationprobiotic fold level>2 a to significantly up-regulated be to a resultofhighfatfeeding.Occludinwasfound the increased intestinal permeabilityobserved as in HFDfedgroup,whichisconformitywith both thetightjunctionproteinscould be observed Figure :Ageassociatedimbalancein immunological parameters ofmice Mice Mice Th aging micewhichconclusivelysuggestedaskewed aggravation of humoral immunity was evident in immune response,chronicinflammationand intestine. Inconclusion, a declineincellmediated in increasedIgG1/IgG2aratioandIgElevels towards Th shift remarkable a showed IL-10) IL-4, (IFN-γ, (A1A1 andA1A2)BCMs (BCM-5 andBCM-7) It wasfoundthatfeedingof A1“like”variants peptides. synthesized commercially and β-casein intubated withrespective geneticvariantof parameters, experimental micewereorally BCMs (BCM-5 and BCM-7) on gutimmunological feeding A1 and A2 “like” variants of β-casein and of effect the visualize to order In β-casein. of was alsonotdetectedfromanyofthevariants BCM-5 of release Likewise, β-casein. of variant release of BCM-7 could not be detected from A2A2 However, β-casein. of variant A1A2 than more A1A1 variantwasobservedtobealmost3.2times from β-casein) mg/g (0.20±0.02 BCM-7 of yield competitive ELISA. The the presenceofBCM-7by revealed milk from isolated β-casein of variants leucine aminopeptidase from A1A1 andA1A2 Further hydrolysis of fractionBwithelastaseand was observedinfractionAbyMS-MSanalysis. sequence whilenosuchpeptideorprecursor acid peptide(VYPFPGPIHNSLPQ)havingBCMs fraction (B)showedthepresenceof14amino and competitive ELISA. OneoftheRP-HPLC to standardBCM-5andBCM-7byMS-MS HPLC collectedatRT24and28mincorresponding peptide fractions (A and B) of preparativeRP- in two of BCMswerecarriedout vitro. Detection enzymes (Pepsin,TrypsinandChymotrypsin),in usingproteolytic digestion gastrointestinal simulated animals andsubjectedtohydrolysisby from milk samples of genotyped KaranFries isolated were β-casein genotypes. A2A2 and cattle (KaranFries)withexistenceof A1A1, A1A2 alleles of β-casein was detected in Indian crossbred Restriction Site).PresenceofA1andA2“like” of β-casein with PCR-ACRS (Amplification Created cattle were screened for A1 and A2 “like” variants cattle breeds.Nativeaswellcrossbredbreedsof abroad andneedstobevalidatedinindigenous bymany researchers digestion hasbeenestablished release fromA1“like”milkduringgastrointestinal depending upon controversial evidences. Their diseases andneurological 1diabetes diseases, Type heart disorderslike inmany implicated be to postulated been have (BCMs) β-casomorphins effect onGutImmunity their and Milk from β-casomorphins of Release 2 pathway duringaging. 2 response,whichfurtherresulted derived from a kDa. ThesepeptideA,B,CandD(coded)were peptides, of which four peptides were less than one analysis of11thfractionshowedthepresence15 highest ACEI and antioxidant activity. LC-MS/MS on RP-HPLC, 11th fraction of less than one kDa has 2.82±0.04 TEAC,respectively.Outofthe16fractions and 83.0±1.53%/50µg of activity antioxidant and fraction less than onekDapossess highest ACEI Ultrafiltration respectively. hydrolysates), of mg (µmol/ TEAC 0.06 ± 1.759 and µg 2.23%/50 ± showed ACEIandantioxidantactivityof72.55 Trypsin hydrolysates at 15% degree of hydrolysis or incombinationfordurationof3hours.Pepsin– hydrolyzed bythedigestiveenzymeseitheralone The casein was isolated from buffalomilk and Osteogenic PeptidefromBuffaloMilk high affinityforBCM-7. dissociated constant innanomolar range indicating aptamers aregrouped.Someofexhibited on presence of specific structural motif in aptamer, SELEX for obtaining high affinity aptamers. Based long ssDNA.Theprocessrequiredseveralround from 10 detection ofBCM-7.Theselectionhasbeenmade for their use in development of aptamer based Several aptamersaregeneratedagainstBCM-7 expression of osteoblastic differentiation marker expression ofosteoblasticdifferentiation Peptide Cenhanced the activity. C enhancedthe phsophatase activity of calvarial cells while peptide Trypsin hydrolysatesofcasein reducedalkaline of 50µg/ml and 30ng/ml, respectively. Pepsin– rat calvaria cells significantly at the concentration casein andpeptideCenhancedtheproliferationof other peptides. Pepsin–Trypsin hydrolysates of the highestACEIandantioxidantactivityamong κ-Casein, respectively. Synthetic peptide C showed through Th peptides induce immune response in mice gut consumption A1 “like” variant of β-casein or BCM The presentstudyclearlyindicatesthatindividual respectively. peptides and variants β-casein the numbers wererecorded on consumptionofany However, nochangesinsIgA,IgA+andgobletcell BCM peptidesthanrespectivecontrolgroups. individual consumption of A1 “like” variants and onTLR-4 alsoincreased(P<0.01)remarkably than controlgroups.ExpressionsofTLR-2and observed in intestinal fluid in experimental groups 1, IL-4, total IgE,IgG,IgG1andIgG2awerealso increased levelsofMCP- Likewise, considerably intestinal tissuethanrespectivecontrolgroups. in remarkably infiltration leucocyte and (MPO) individually increased (P<0.01) Myeloperoxidase 13 - 10 2 pathway. 15 random sequences of 72 nucleotides randomsequencesof72nucleotides s 1 -Casein, a s 2 -Casein, β-Casein and –– AnimalBiochemistry ––

35 ndri annual report 2013-14 36 ndri annual report 2013-14 osteoporosis. enzymes could be a potential target to fight against from buffalocaseindigestedbygastrointestinal vitro, respectively.Therefore,peptideCreleased rat calvariumosteoblastcellsandosteoclastsin acts as osteoanabolic andantiresportiveagenton Peptide CwithACEIandantioxidantactivity reduction inTRAPpositivecells,respectively. 45% and 55% showed C peptide and casein of to thatofcontrol.Pepsin-trypsinhydrolysates while thatofcaptoprilandtroloxweresimilar marker genesandosteoclast differentiationthe expressionofosteoblastic Pepsin–Trypsin hydrolysatesofcaseinreduced 70%. upto K cathepsin and TRAP genes, marker Peptide Csuppressed theexpression of osteoclastic osteocalcin by1.57,1.47and1.78folds,respectively. genes, collagena –– AnimalBiochemistry –– and bileextracttherelease offreefattyacids fat) digestion in thepresence of pancreatic lipase (3% milk the for conducted was digestion In vitro Milk FatDigestibilityofDairy Animals TRAP positivecells(yellowarrow),negative (green arrow),Thebonemarrowcellsshowingfusion Figure :Bonemarrowcellsculturedonmonolayerof Figure :Calvariacellsonconfluency 1 type I, alkaline phosphatase and 1 typeI,alkalinephosphatase and calvaria cell (blue arrow)

mmol mL buffalo milkwere146.0,136.8,118.5and102.5 fatty acidsreleasedfromcamel,goat,cowand h ofpancreaticlipasedigestion,thelevelsfree the Confocal Laser Scanning Microscopy. After5 by size atdifferenttimeintervalswasobserved the digestionprocess, the changeinfatglobules were measured bypHstat titrationmethod. During and buffaloes,leadingtoinfertility bydisrupting most common endemic diseases of dairy cows Uterine infectionafterparturition isoneofthe Buffalo Ovary LPS InducedRegulation ofCyp19Genein in theorder:camel,goat>cowbuffalo. increased. Therefore, digestibilityofmilkfatwas lipid digestionasthesurfacetovolumeratiois smaller thesizeoffatglobules,fasteris that indicated This figure). (see milk μm) (1.1-2.1 camel and μm) (1.1-3.9 goat μm), (1.6-4.9 cow μm), (3.9-7.7 buffalo in range size different the the fatglobules. Fat globuleswere observed with milk fatwasfoundtobedependedonthesizeof goat andcow milk (P<0.01).Thedigestibilityof buffalo milk was significantly lower than in camel, digestion in and camelmilk,whereasfat in goat digestion in cow milk was significantly lower than fat digestionatthis pointofdigestion,butfat difference (P <0.05)betweengoatand camel milk at differenttimeintervalsduringpancreaticlipasedigestion. Figure: Confocalmicroscopyimagesoffatglobulesmilk (A) camel(B)goat(C)cow(D)buffalo.Emptyparticles, flocculation andcoalescenceareindicatedwitharrows -1 , respectively. There was no significant of Cyp19inmouse. for theformationofcorpuslutumandregulation leucinezipperproteins,isnecessary family ofbasic a of member a C/EBPβ, regulation. down Cyp19 the study explored role of CEBPβ in LPS mediated of control. Tounderstandthemolecularmechanism than lower significantly be to found were gene Cyp19 gene expression and estradiol production production. Interestingly, resultshowed that both expression andestradiol evaluated onCyp19gene cultured anddosedependenteffectofLPSwas on ovarian functionin vitro, granulosa cells were effect ofLPS to seethe to normalanimals.Further, Cyp19 geneexpressioninleucocytesascompared mucopurulent mucus have low copynumberof animalswith showedthat inleucocytes transcripts producing animals.ExpressionanalysisofCyp19 of estradiolascomparedwithclearmucus flecks mucus have lower peripheral concentration showed thatanimalswithmucopurulentwhite in plasmaofanimalswasmeasured.Results off-white pus in mucus, respectively. Estradiol or flecks white and mucus translucent or clear have beencategorizedbasedonthepresenceof Morphologically, normal and infected animals of postpartuminfectedandnormalanimals. tract andjugularveinwerecollectedafter21days infected animals, mucus and blood from theuterine is notknown.Toclassifytheinfectedandnon- enzyme inestrogenbiosynthesis, aromatase, key induced downregulationofCyp19 to LPS.However,molecularmechanism of LPS are amongst prominent ovarian dysfunction due lower peripheralplasmaestradiolconcentrations Cyp19 genetranscripts ingranulosacells and impair ovarianfunctionandovulation.Reduced for directactionofLPS in theovarianfollicle to showed thatgranulosacellshaveamechanism uterine andovarianfunction.Recentreports was found to increase expression of C/EBPβ. LPS granulosa cellsat24hrwhile atthesametimeLPS LPS (1μg/ml) down regulated arrows indicatethelacalizationof C/EBPβintonucleus Figure: Effect of LPS and OxPAPC on nuclear levels of C/EBPβ Cyp19 genedownregulationingranulosacells, expression in Cyp19 expressionin gene, encoding gene, encoding study included up regulating genes suchasFRZB, study includedupregulating Validation factors. transcription identified the by identify thegenomewidenovel genesregulated to differentiallyexpressed genes,andalsoto to explore thenovel transcription factors related Promoter AnalysisPipeline(PAP)andDAVID the datausingbioinformaticssoftwareslike differentially expressed genes, butalsoanalyzed The presentstudynotonlyvalidatedsome this problem. to response host the understanding for identified and endometritisuterinetissuesinbuffaloswere normal regulated and76down-regulated)between chip, 125differentially expressed genes (49up- Microarray analysisusingaffymatrixbovine Endometrial Genes Associated with Endometritis Genome-wide DifferentialRegulationof transcription factorsC/EBPβ. down-regulation ofCyp19genecould be though of the present study showed that LPS mediated production during infection. In conclusion, results turn decreased Cyp19 genemessage and estradiol promoter andtransdeactivatethegenewhichin Increased nuclear C/EBPβ binds with Cyp19 gene translocation tonuclearthroughTLR4 signaling. its and expression β CEBP regulate up LPS that, LPS. From these present results, itcan be concluded increased when granulosacellsweretreatedwith be to found was C/EBPβ of localization nuclear EBP with and without LPS treatment showed that signaling. Inaddition,immunolocaliaction of C/ were treatedwithinhibitorofTLR4receptor control. These effects were reversed when cells enrichment) onCyp19promoterascomparedto (2.5.fold binding C/EBPβ increased LPS that showed results assay immune-precipitation Chromatin control. with compared as C/EBPβ treatment togranulosa cells increased the nuclear Figure: Genome-widedifferentialregulationofgenes associated withuterineinfection –– AnimalBiochemistry ––

37 ndri annual report 2013-14 38 ndri annual report 2013-14 decrease was comparatively less in the spermatozoa decrease wascomparativelyless inthespermatozoa of compared tothefreshspermatozoa. Theextent spermatozoa showed less abundance of Bcl2 detected byWesternBlot. Thecryopreserved Bcl2 andproapoptoticproteins BaxandBakwere spermatozoa (63.23%) . The anti apoptotic protein decreasedincryopreserved was foundtobe Sperm mitochondrialmembranepotential two extenders. cells (8 & 9%) were found to be comparable in the necrotic and 32%) & (41 apoptotic late 5.0%); & early apoptoticinSoyavs eggyolkextender(4.0 conventional eggyolkextender.Thenumberof and cryopreservedinsoya milk extenderand splitted intofresh + 3.0massactivity more than made onfreshlycollectedbuffalosemenof Comparative studies on apoptotic markers were Buffalo (Bubalusbubalis) Apoptotic MarkersinFreshandCryopreserved mir-6816, mir-125b,Let7b-5p,mir125a,miR125b. miR-23b-3p, miR-6089, 127-5p, miR-7193-5p,bta-miR-125b, miR-2898, mir5924,miR-6871,miR-2890,miR- miR-22-3p, are: miRNA identified The miRNA. clones, identifiedsomeconservedandnovel Sequence analysisofsmallnumber ofrandom miRNA inbuffalocorpusluteumwasstudied. In thepresentstudy,cloningandanalysisof that regulate gene expression post-transcriptionally. MicroRNAs (miRNAs) aresmallRNAmolecules Micro RNAfromBuffaloCorpusLuteum which isafascinatingissueforfuturestudies. endometrial tissue of animals with endometritis, TEL2 geneexpressionwasdown-regulatedin endometritis uterinetissues. Interestingly, the PAP analysiswasvalidatedbetweennormaland using identified TEL2 factor transcription novel 4439 genome-widenovelgenes.Additionally, Further PAPanalysisrevealedthatTEL2regulates genes involvedintheimmunological genecluster. transcription factorregulatingtheup-regulated software identified cluster fordown-regulatedgenes. ThePAP gene for upregulatedgenesand clusters includingimmunologicalgenecluster several identified analysis DAVID respectively. regulated and down-regulated geneexpressions, the microarray and validation studies for up- between correlations 67% and 79 were there ODC1 duringendometritis.Itwasobservedthat genes suchasFOLR1,FBP1,CCNB1,PSAT1, CHST9, GIMAP7, GFRA2anddown regulating –– AnimalBiochemistry –– miR-5924a-3p, TEL2 as a significant (p < 0.05) Spermatozoa arginine metabolism miR-4581, miR-125b, spermatozoa cryopreserved in SMT compared to spermatozoa cryopreserved in SMT compared to in better (p>0.05) significantly be to found were exrender oncryopreservation. Theotherparameters yolk ascomparedtotheegg acrosomal integrity sperm motility,viability,membrane integrityand showed no significant differences (p>0.05) in and proteintyrosinephosphorylation.SMT anion, lipid peroxidation; extentof cryocapacitation Oxidative stressvizgenerationH intracellular Ca+2,1,2diacylglycerolandcAMP; choline, vizlysophophatidyl secondary messengers phospholipid ratio;generationofintracellular integrity; membranearchitecture;cholestrol/ membrane integrity,viabilityandacrosomal lethal damagewasmeasuredaspostthawmotility, cell deathaslethaldamage.Thereversiblesub- cause ofthecell death compared tothenecrotic (SMT). The apoptotsis was found to be the major Extender (EYT) and newSoya Milk Extender were comparedwiththeconventionalEggYolk and sub lethal damages due to cryopreservation in theconventionalEggYolkExtender.Thelethal yolk egg of (v/v) 20% of place in Fructose) mM (pH 7.0,274mMTris,87Citricacidand43 Buffer Citrate Tris in Milk Soya of (v/v) 25% of iscomposedwas developed.Thenewextender extender called Soya Milk Tris Extender (SMT) use ofeggyolkinsemenextenders,aphytobased presence ofantifertilityfactorsassociatedwiththe composition, riskofxenobioticscontamination, To overcometheinherentdrawbackslikevariables and Bovine(KaranFries)Spermatozoa of Buffalo Cryopreservation Based Extenderfor Soya MilkTrisExtender (SMT): ANewPhyto compared tothefreshspermatozoa. found toincreaseinfrozen/thawedspermatozoa various caspasesandDNAfragmentationwas in cryopreservedspermatozoa.Theactivitiesof activated infreshspermatozoawasnotdetected compared to the Soya extender. However, AIF significantly higher (p> 0.05) in egg yolk extender indicator ofcaspaseactivationwerefoundtobe regulatory protein for apoptosis and PARP an mitochondrial a C, Cyt. 100%. as spermatozoa the soya milk extender (75.11 ± 5.55%) taking fresh extender comparedto yolk cryopreserved inegg spermatozoa in 4.90%) ± (110 higher significantly 3, caspase8and9werefoundtobe spermatozoa. The relativeabundanceofcaspase found tobeincreasedoncryopreservationof (15 kDa)andcaspase9precursor(50kDa)were 8 precursor(58kDa)alongwithcleavedfragment cleaved fragment ofcaspase 3 (27kDa);caspase to theuse ofeggyolk extender. An activated cryopreserved using soya milk extender compared 2 O 2 , superoxide , superoxide the fieldlevelforitscommercialization. at validation requires and efficient more and free The technologyissimple,cheap,contamination semencryopreservation.for buffaloandbovine substitute toreplacethecommercialEYTextender 10X) are prepared and these prove to bea better based formulationsinconcentratedforms(3Xand 30 daysunderrefrigeratorconditions. Two soya extender was found to increase the shelf life up to bisulphite ata concentration of 0.2 mg/ml of the temperature. Thepreservativepotassiummeta of itsshortshelflifeoneweekatrefrigerator requires frequent preparation of soya milk because The problem with use of SMT extender in the field the extendersbutinabetterwaySMT. the qualityofcryopreservedspermatozoainboth trehalose and taurine, further significantly improved the EYTextender.Supplementationofadditivesviz Dr. A.K.Srivastava,Director,NDRI receivingAgriculturalLeadershipAward-2013 phosphatase) wereexaminedatthe7 (Aspartate aminotransferase) and ALP (Alkaline like ALT(Alanineaminotransferase),AST level andenzymemarkersforliverdamage MSNs onbodyweightindex,totalprotein intraperitonial (IP)infusion ofMWCNTs and animal modelwasstudied.Theacuteeffectof doses aswellroutetakingalbinomicean MSNs byacomparativeassessmentatdifferent thickening. InvivotoxicityofMWCNTsand hyperplasia, localizedalopeciaandskin oxidative stress,glutathionedepletiondermal Mice exposedtounpurifiedCNTsexhibited Toxicity ofNonmaterial is higherthanMSNs. ofMWCNTs wasconcludedthattoxicity which it MSNs displayed similar toxicity levels, based on that MWCNTsevenatmuchsmallerdosesthan treated MWCNTs group. The results showed group. Total protein level was highinorally pattern especially inMWCNTs (IP)treated enzymes assayed,ASTdisplayedapeculiar observed amongdifferentdoses.Ofthree were levels enzyme in differences significant No doses. all at insignificant statistically were Changes in organ coefficient of different organs 2.0 mg/kg and2.5mg/kg BW ofMWCNTs. and 50mg/kgofBWMSNs1.5mg/kg, intraperitonially with10mg/kg, 25mg/kg with thesingledoseof50ppmMWCNTsand after exposure.Themiceweretreatedorally –– AnimalBiochemistry –– th day

39 ndri annual report 2013-14 40 ndri annual report 2013-14 Buffalo α Buffalo hydrolysates a) Antioxident characteristics ofbuffalo a curcumin atadoseof30mg/kgbodyweight stress inmice.Treatmentwithnanoencapsulated curcumin against paracetamol induced oxidative (Size: 141.6±15.4nm)overunencapsulated therapeutic benefits of nanoencapsulated curcumin The present study was conducted to validate the nanoemulsions ofcurcumin in mice model: of activity antioxidant of Assessment Human Health Improved and OtherBioactiveCompoundsfor Foods withPrebiotics,Probiotics,Micronutrients Research onNutraceuticalsfromMilk,Functional ml after8hofhydrolysis at 1:100 E:S ratio. For corresponding peptideyield was1.72±0.07mg/ 0.05µmol trolox equivalent per mg peptide and optimum ABTS° origin aloneandincombination. Foralcalase flavourzyme) and (alcalase bacterial and pepsin) proteolytic enzymes of animal (corolase pp, EC- emulsionwasgivenatadose30mg/kgBWfor4daysafterparacetamoladministration for2days, CP -curcuminwasgivenatadose30mg/kgBWfor4dayspriortoparacetamol administration 2) C: Controlmicewithoutanytreatment,PParacetamoltreatedatadoseof300mg/kgBWfor2days, 30 mg/kgBWfor4daysafterparacetamoladministration2days:Thevalues wereexpressedasMean±SEMfor8micepergroup Emulsion administrationorally1) s -casein was hydrolysed by commercial + radical activitywas2.40 ± a–e EP -emulsionwasgivenatadose30mg/kgBWfor4dayspriortoparacetamol administration 2) Means withdifferentsuperscriptsdiffersignificantly(P<0.05) D airy s -casein -casein C hemistry stress inducedcelldamageandassociateddiseases. the preventionaswelltreatmentofoxidative nanoencapsulated curcumincanbeutilizedfor of improvedbioavailability the that suggested Results action. overalldoserequiredforthe the from theencapsulatedparticlesthusreducing reduced sizeaswellslowreleaseofcurcumin to the nanoencapsulated curcuminwasattributed against oxidativestress.Theenhancedactionof curcumin atthesamedosefailedtoofferprotection oxidative stress. Whereas, unencapsulated significantly attenuatedparacetamolinduced both inpreventiveandcurativegroupsofmice CC -curcuminwasgivenatadose Unencapsulated curcumin Encapsulated curcumin -1) :

stress generated by 5 mMH stress generatedby and passed through 3 KDa filter reduced oxidative casein hydrolysatespreparedwithAlcalaseenzyme viability by 50% during 20 h of incubation. Buffalo of buffaloα b) Cellularevaluationforantioxidativepotential respectively. mg/ml and1.04±0.01ofhydrolysate, and correspondingpeptideyieldswere3.96±0.01 3.68 ± 0.02 µmoltroloxequivalentpermg peptide 0.03 µmoltroloxequivalentpermgpeptideand and LYQGPIVLNPWDQVKR from α and VALINNQFLPYPYYAKPA) fromk-casein ß-casein, two peptides (LINNQFLPYPYYAKPA fromand SKVLPVPQKAVPYPQRDMPIQAFL) Three peptides (VLGPVRGPFPIIV, GPVRGPFPIIV and 8was42.4,33.3248.32µg/ml,respectively. concentration forfraction2,3 immuno inhibitory 0.40 -1.5µg/ml.The fractions rangedbetween that addition of5mM ofH plate method andexpressed intermsofLogN plate activity againstE.Coli ml. Eachfractionwasassayedforantimicrobial of differentfractionrangedfrom95-204µg/ resolved into 12major peaks. Theproteincontent NCDC14 L.acidophilus milk hydrolysatesby chromatogram of the eluentobtainedfrom skim skim milk,wereseparatedoverRP-HPLC.The growth forLactobacillusacidophilusNCDC14in peptides producedunderoptimizedconditionof from Characterization ofantimicrobialpeptides viability at666.6ug/mlconcentrations. KDa cut off membrane showed 75% increase in cell 3 through filtered and PP Corolase by produced by 80% cell viability. In case of casein hydrolysate 62.5 µg/mlconcentration, which can be exhibited flavourzyme optimum ABTS° cells by H oxidative stressinCaco-2 Method forgenerating N optimum ABTS° flavourzyme, with alcalase and pp corolase with mg/ml of hydrolysate. When pepsin was used and correspondingpeptideyieldwas1.36±0.01 2.45 ±.04µmoltroloxequivalentpermgpeptide at 1:50;E:Sratio, ABTS° 2hofhydrolysiswithpepsin of1:5.After ratio 1.99 ±0.01mg/mlafter6hofhydrolysisatE:S peptide andcorrespondingyieldwas was 2.24±0.04µmolTroloxequivalentpermg hydrophobicity identified from fraction 2, 3 and 8. 35-50% with peptides cationic major be to found f value.Theantimicrobialactivityofdifferent L. acidophilusNCDC14:Antimicrobial 2 O s 2 -casein peptides wasstandardized. It wasobserved + radical activitieswere1.75± ATCC25922usingthetotal + radical activity was 2 O 2 O 2 + in Caco-2cellsat 2 radical activity decreased cell s2 casein were casein were 0 / the bioavailabilityofcalciumandvitaminD of fortified milk. When used in combination, andphysicochemicalanalysis sensory after while responsetimewas2-3minincaseofmilk of adulterated milk containing hydrogen peroxide colour changedtodeeppinkimmediatelyincase strip. The visualization ofchangeincolourthe a dropofmilkonthestripfollowedby putting and hydrogenperoxideinmilk,thetestinvolved original yellow colour. For detection of glucose while inpuremilksamples, the stripretainedits neutralizers (immediately)andurea(after2min) the stripchangedtodeepredinmilkcontaining visualization of colour of the strip.The colour of dipping thestripinmilksamplesfollowedby neutralizers and urea in milk, the test involved were developed and validated. For detection of urea, glucose and hydrogen peroxide in milk milk: in andcontaminants selectedadulterants for Development ofstripbaseddetectiontests Biotechnological Concepts through Application ofNewerChemical and Ensuring QualityControl Generation Toolsfor Health ConcernsandDevelopmentofNew Clean Milk ProductionwithaFocusonEmerging and yoghurtfromfortifiedmilk. possibility of manufacture of good qualitydahi milk did notdiffer from control which suggest A alone. Dahi and yoghurt prepared from fortified vitamin A than milk fortified with iron or vitamin iron resultedinbetterbioavailabilityofand Vitamin Aalongwith the nutrients. both between increased whichindicatedapositiveinteraction ppm) and vitamin D dibasic andcalciumcitratetetrahydrate(600 IU/L); two calcium salts viz.calcium phosphate hydrate (25 ppm) and vitamin A acetate (2500 pyrophosphate solubleandferrousgluconate calcium and vitamin D Toned milk was fortified with iron and vitamin A; milk: fortified micro-nutrient of Development Figure: Variousstepsinthedetection ofaddedureainmilk Four stripsfordetectionofneutralizers, using strip 2 2 (600 IU/L) were selected . Two iron salts viz. ferric –– DairyChemistry –– 2

41 ndri annual report 2013-14 42 ndri annual report 2013-14 by using RP-HPLC over C achieved be could β-sitosterol) and (stigmasterol The separationofcholesterolandphytosterols the detectionofvegetableoils/fatsinghee RP-HPLC and RP-TLC protocolsdeveloped for are rapidandresultsavailablewithin5min. glucose and 0.02% for hydrogen peroxide. for The tests 0.03% urea, added for 0.06% neutralizer, for 0.04% was which ascertained was strip these of only lightpinkcolourappears.Thesensitivity containing glucose.Incaseofnegativesamples, –– DairyChemistry –– not very clear. By using this standardized protocol, not very clear. By usingthisstandardizedprotocol, was β-sitosterol from campasterol of separation the though β-sitosterol, and cholesterol between was 0.16. The Rf value of campasterol was in- was calculated as 0.19 whereas that of β-sitosterol TLC plates). The Rf valueof cholesterol standard chromatographic plates(RP-18silicagel-GF also achievedbyusingreversephasethinlayer The separation of cholesterol and β-sitosterol was up to 2% level could be detected in ghee samples. oil up to 1%; refined sunflower and groundnut oil revealed that coconut oil up to 5%, refined soybean oils. AnalysisofUSM of adulteratedgheesamples detection ofadulterationgheewithadulterant could beused as amarker phytosterol forthe β-sitosterol that revealed oils adulterants pure of RP-HPLC analysis of unsaponifiable matter (USM) C: 1%groundnutoilinghee; A B A: cholesterol+β-sitosterol; B: Pureghee; ghee; E:5%groundnutoilinghee C D: 2%groundnutoilin 18 column at205nm. D E 254 S milk was enzyme concentration of 5 U/g of protein milk was enzyme concentration of 5 U/g of protein were optimized.Theoptimized conditionsforcow linkage of proteins in both cow and buffalo milk conditions fortransglutaminase catalyzedcross rheological propertiesofDahi wasstudied.The effect ofthecrosslinkingmilkproteinon from differentspeciesusingtransglutaminaseand was optimizedforthecrosslinkingofmilkprotein Process and dahipreparation: proteins milk treated Physico-chemical propertiesoftransglutaminase during thisprocess. increase to found was acidstemperature.content free fatty However, 4 days 10 5 and for burfi and days) at room duringalso (khoa for their subsequent storage and Buffalo) and (Cow milk raw from burfi and milk constants of during fat khoa ofpreparation physico- the in negligible, almost chemical changes, small very only are there that revealed results wereassessed.The storage subsequent their during and burfiand khoa into milk of conversion during constants fat milk in place khoa/burfi: of preparation Physico-chemical changes ofmilk fat during adulteration atallthelevelswaseasilydetected. versa usinggasliquidchromatography(GLC), to C and AST. On thebasis of the ratios of sum of C limit incaseofBRreading,RMvalue,Iodinevalue revealed thatfractionationreducedthedetection into twoconsecutive solid and one liquid fraction mixture adulteratedgheesamplesat15 level inboth types of ghee.Partitioning of pureand liquificationtime (CLT)test could detect BBF at 5% ghee andBBFinbuffaloghee,whilecomplete cow in SO of 5% as low as detecting for suitable Apparent solidification time (AST) test was found fat wasnotdetectedatanyofthelevelstudied. 10% level in both types of ghee, while animal body types ofghee.Iodine value could detect easilyat could be detected individually at 10% level in both adulterants the Reichert-Meissl (RM)valueboth cow ghee and 15% in buffalo ghee. Similarly using can be used for detection of vegetable oil at 10% in revealed thatButyro-refractometer(BR)reading various physico-chemical properties. The study as well as in their combinations, were analyzed for added inpurecowandbuffaloghee,individually ghee: Detection of soybean oil and buffalo bodyfatin addition in ghee could be detected up to 7.5% level. oil coconut and level 1% to up detected be could oil groundnut and oil sunflower oil, soybean as the adulteration of ghee with adulterant oils such with adulterant adulteration ofghee 14:1 Soybean oil (SO) and buffalo bodyfat(BBF)

/ sumofC 15:0 to C 20:0 fatty acids andvice- fatty The changes taking taking changes The ° C and10 ° 4:0 C acceptable till 90 days found sensorially were variants others sorghum variant, all and 30ºC.Exceptthe were evaluatedat4ºC formulated products Shelf lifeoftheall RDA. of fibre dietary of 12-19% and A vitamin of 13-14% calcium, of 19-33% ofiron,respectively) men and women, adult (in 15-21% and 16-22% approximately provided product g 200 of serving a revealedthat product the of profiling the productvaried from 152 to352cP. Nutritional Methodology foreachvariant.Theviscosityof levels were optimized through Response Surface Levels of germinated flour, pectin and sugar fears ofgreengram,chickpea,ragiandsorghum. of smoothie were prepared, using germinated prepare shelfstablesmoothie.Fourvariants Process and ingredients were optimized to Ready-to-serve BreakfastSmoothie k-CN> αs β-CN> was linkage cross of order the milk, cow and forbuffalomilk,itwas3U/gofprotein.In milk and double toned milk. The water holding holding Thewater tonedmilk. milk anddouble properties of dahi prepared from cow/buffalo skim andsensory rheological improvedthe treatment cross linked followed by β-CN. Transglutaminase at higherdegreeofpolymerizationk-CNgetsmore polymerization, similartrendwasobserved,while 1 -CN. But in buffalo milk at low levels of -CN. Butinbuffalomilkatlowlevelsof D airy T echnology level (9% & 4%), LDL-cholesterol level (21% level LDL-cholesterol 4%), & (9% level Significant decrease (P≤0.05) in total cholesterol (treatment-2). cholesterol 1% without weeks 8 continued withremaining animals (6each)for adipose tissue weight were measured. Study was serum, liverandfatextracted fromlivertissue, of profile lipid their and sacrificed were group (treatment-1). Afterthe8weeks,4ratsineach weeks 8 to up cholesterol 1% containing (FD) oil (SD), normal butter (ND) and fortified butter into threegroups(10each)andfedonsoybean months. Theanimalswererandomlydivided two of period a for fat 25% and cholesterol 1% containing adiet feeding by hypercholesterolemic Thirty Wistarrats(120±20g)weremade respectively. 5%, and 12% of rate the at acid with phytiosterol(PS)andconjugatgedlinoleic Butter was prepared by churning cream fortified Production ofFunctionalButter above ratedthisproductasexcellent. buy the product at Rs. 20/- per 200 g and 40% and to willing were consumers of 86% that revealed A consumersurveyreport 16.13 per200gbottle. the optimizedproductvariedfromRs.15.72to at 4ºCand 60 daysat30ºC.Manufacturingcost of up to83%. treatment improvedthewaterholdingcapacity TheTGase control. than gelstrength better in resulted milk goat in level 3% at caseinate further improvethesame.Additionofsodium properties; however,TGasetreatmentdidnot resulted inimprovementofgoatmilkgelation (0.3%) pectin and (1.5%) WPC of addition on of acidgelbyfermentationgoatmilk(Dahi) functional propertiesofgoatmilk.Preparation treatment ofgoatmilk resulted inchangeof Transglutaminase β-CN>κ-CN>α-CN. was linkagecross of order the milk, goat In 67%. of linkage ofgoatmilkproteinsresultedtothelevel Under theseconditions,theextentofcross- incubation temperature of40 enzyme concentrationof5U/gprotein, was milk forgoat condition The optimized refrigerated temperaturewasupto2weeks. improved. Thestoragestabilityoflowfatdahiat also were product final the of consistency and in lowfatbuffalomilkdahi.Syneresis,appearance toned milkdahi,thesevalueswerealmostdouble cow skimmilkdahiandupto1.8Nindouble % in double toned milk dahi, firmness up to 1.1 N in 90 upto and dahi milk skim in % 85 upto capacity 0 C for4hours.

43 ndri annual report 2013-14 44 ndri annual report 2013-14 stored atrefrigerationtemperature(4±1 sugar syrup, withandwithout samples packet scores of7.0and6.40respectively.Moreover, had shelflifeof30dayswithoverallacceptability syrup, storedatambienttemperature(30± 1°C) 30 ±1°C.Samples packed withandwithoutsugar with andwithoutsugarsyrup stored at 4and then packedinstandup,whiteopaquepouches was carried out using RSM. The optimized product of weight by (% viz., sugar syrup concentration(ºBrix), semolina After that,optimizationofindependentvariables Mohan . suitable formanufactureofqualityKheer type andstrengthofcoagulanttoproducechhana level offatincowmilk followedbytheselectionof Preliminary trailswereconductedtoselectthe Kheer Mohan treated ratsfedwithfunctionalbutter. noticed inliverandmammaryglandsofDMBA and uridinediphosphoglucuronosyltransferase γ-glutamyltranspeptidaseenzymes detoxifying functional butter.Increasedactivityofcarcinogen conducted to study anticarcinogenic effect of profile ofserum.Animalstudieswerealso lipid performedwellincontrolling butter fortified that revealed Study respectively. ND recorded inFDgroupascomparedtoSDand were 17%), & (48% index atherogenic 9%), & –– DairyTechnology –– (MicroGARD WPC based MFCS presence with biopreservative protein, 2.79% lactose, 18.67% sugar and 0.79% ash. standardized method contained 37.87% fat, 6.54% ash whereas MFCS based on WPC obtained using 1.37% and sugar 19.94% lactose, 8.94% protein, standardized method contained 21.36% fat, 5.17% product. MFCS based onBMS obtained using solid (BMS) were optimized for formulation of whey proteinconcentrate(WPC)orbuttermilk Various ingredientsi.e.,butterfat,oliveoiland functional dairy and non-dairy ingredients. of Mixed-Fat Chocolate Spread (MFCS) using Technology wasdevelopedformanufacture Mixed-fat ChocolateSpread than themarketcostofproduct(` pack size was Net manufacturingcostoftheproductin500g ofpreparation. method traditional compared to as achieved was time cooking in reduction 50% at ambienttemperature.Instandardizedprocess, to 6daysobservedshelflifeofthemarketsample scores of7.0and6.0,respectivelyincomparison shelf lifeof60dayswithoverallacceptability compared toMFCSwithout preservative.The TM from CowMilk 80 which was significantly lower significantly was which 80 ` ) hadslowerrateofspoilage as chhana) andcookingtime(min) 120for500g). ° C) had

Shigella anti-diarrheal effectafterchallenging withShigella more and activity β-glucuronidase in reduction superior anti-carcinogenic effect evidenced by lower coliformcount,higher faecallactobacilli, efficiency ratio and apparent protein digestibility, protein ratio, efficiency food gain, weight better trials onmiceledtothefollowingconclusions: sensorily acceptableupto18days.Invivofeeding during 30days of storage.The beverage remained (11-12 log cfu/ml) reduced to 6.36±0.35 log cfu/ml (Ca, Fe, Zn, Mg and P). The high probioticcounts digestibility andthebioavailabilityofminerals simultaneous increase in protein and starch with (46%) polyphenols and (78%) acid phytic in inreduction resulted probiotic the by Fermentation extract. malt barley liquid and flour millet pearl based onwhey-skimmilkmixture,germinated used in preparation of a probiotic beverage A dairyisolateprobioticLactobacillusstrainwas Milk ProbioticBeverage Germinated PearlMilletContainedWhey-Skim growing functionalfoodmarket. This product has greatpotential inthefastest acceptable for28dayswithoutanypreservatives. sweetened soft cheese. The product remained functional the for finalized were FOS and inulin functional ingredients.Optimumlevelsofcocoa, and cocoaas inulin fructooligosaccharide (FOS), incorporating wasdevelopedby milk buffalo Sweetened functional soft cheese from Sweetened FunctionalSoftCheese very usefultotheconsumersaswellindustry. (p significantly time, livercholesterolandtriglyceridedecreased same the index decreasedand at and atherogenic observed that serum total, LDL, VLDL cholesterols were fedtohypercholesterolemicratsanditwas its hypocholesterolemic effect, developed products sound. Tovalidate and functionally nutritionally productitselfwas andthe and spreadability flavour its to due consumers the by much very consumers) revealedthatthePCSwasliked effect. Theconsumeracceptancestudy(107 W-3 fattyacidhavingcholesterollowering ingredients viz.,inulin,phytosterolsand cheese spread(PCS)withthreefunctional The study was conducted to enrich the Processed Functional ProcessedCheeseSpread extremely’. was adjudgedas‘likedverymuch’to consumer responsestudyrevealedthattheproduct <0.05). Thetechnologywouldbe 82 submitted to NCBI gene bank). A process of GOS 82 submitted to NCBI gene bank). A process of GOS lactis as strains 14 confirmed of phenotypicandphylogeneticcharacterization characterized uptospecies level andtheresults Chloroform method) and these strains were further SDS, (Using activity β-galactosidase higher 18 outof30strainsKluyveromycesspp.showed SDS, Chloroform and Lysozyme method) whereas (Using activity β-galactosidase higher showed dystenteriae Screening of fifty out of 80 strains of Exhibiting MicrobialStrains Production using GalactosyltransferaseActivity Optimization ofGalactooligosaccharide(GOS) product was 90% of the RDA (360 μg RE per day). the fortifying for finalized acetate A vitamin of amount The day. per mg 9 was fortification for to flavour of the product. Amount of iron finalized electrolytic ironwas more acceptable withrespect based product were also evaluated and found that cereal above of fortification the for iron of salts by pressurecookingprocessingofthebarley.The characteristics over theearlierproductprepared flavour and textural superior have to found was to-reconstitute weaningfood.Theproductformed form. This powdered product was used as a ready- ground inagrinder along withsugar toapowdered wheat was extruded. The extruded product was like processed pearlmillet, WPCandprocessed optimized. Optimizedcombinationofingredients formulation ofcereal based weaningfood were Ingredients andprocessingparametersfor Weaning Food fortified A Vitamin and Iron based Milk-Cereal ` 3.15/100ml. to feeding.Thecostofprobioticproductwas wasinduceddue ormalnutrition No toxicity (accession No.HM473144-48andHM473170- Microscopic imagesofmilkprotein basedmicoremulsionsfordeliveryofherbalextractsinmilkand products in thefermentedsubstrate-fedgroup. K. marxianus and4asK. K. S. thermophilus

TBHQ, 0.02%) antioxidants throughout 21 days21 throughout antioxidants 0.02%) TBHQ, and 0.02% (BHA, synthetic and 1%) extracts tea green and 0.5% (rosemary natural other to conjugated dienes,freefattyacids)ascompared of oxidativedegradationproducts(peroxides, < 0.05) lesseffective in controlling development aqueous) extract was found to be significantly (P incorporated withshatavari(ethanolicand better radical scavenging property. Ghee shatavari at thesameconcentration.Amongtwo antioxidants addedtofatrichmodelsystem tea extracts)andsynthetic(BHATBHQ) compared withnatural(rosemaryandgreen to besignificantly (P<0.05) lower when werefound shatavari ofherb aqueous extracts radical scavengingactivityofethanolicand and potency Antioxidant (84.44±0.18%). and their aqueous extracts (44.33±0.11%), (49.93+ than greater significantly were (86.05±0.13%) ashwagandha (67.70+ (63.22±0.14%), by ethanolicextractsofshatavari model system.Theantioxidantactivityexhibited oxidation coupled acid β-carotene–linoleic the and ethanolic)wasevaluatedat200ppmusing The antioxidantactivityofherbextracts(aqueous Milk Products Delivery SystemforHerbalNutraceuticalsin and Withania Somnifera,AsparagusRacemosus Incorporation of Herbal Components from compared todiabeticgroup. as of coliformswereobservedinGOSfedgroup Higher numberoflactobacilliandlower markers andoverallhealthofGOSfedanimals. improvement indiabetic,antioxidantstress was also standardized. Feeding trails indicated Protocol forthepartialpurificationofGOS was standardized. production, anovelprebiotic Pueraria Tuberose extracts, ethanolicextractsshowed in Milk 0.15) andvidarikhand and Developing –– DairyTechnology –– 0.19)

45 ndri annual report 2013-14 46 ndri annual report 2013-14 reduced calorie, zero trans fat biscuits fromareduced calorie,zerotrans fatbiscuits Process was optimized toformulate composite, Enhanced FunctionalProperties Composite Dairy-cerealbased Biscuitswith www.elearningdt.org.in). NDRI/ Sub-projectWebsite(www.ndri.res.in, to SAUs.Alle-courseswerealsouploadedonthe handed over toNAIP/ ICAR for off-linedelivery course). All49coursesonP-MOODLECDswere also reviewedbyPGstudents(threeper and 6atSVVU.Allcourses(onP-MOODLE)were External reviewer:26coursesatNDRI,17AAU peer reviewed,eachbyoneInternaland Technology) wascompleted.Allthecourses were Deans Committee syllabus for B.Tech. (Dairy of 49e-courses prescribedundertheFourth E-content development through digitalization Programme Development ofe-courses forB.Tech (DT)Degree up to12days bottles, whereas the control lassi had storage life pouches orinpolyethyleneterephthalate(PET) packed inlowdensitypolyethylene(LDPE) a shelf life of 20 days at 4±1°C as bindingmaterial.Thedevelopedproducthad better than the productthatcontainedTween-80 was achievedandtheproductsensorially with observed intheproduct.However,whenadded wascurcumin of retention 92% Tween-80, with component in lassi. Curcumin when added the of retention 50% approximately in resulted of curcumin. Curcumin, when added as such binding for tried was Tween–80 and (β-CD) suitable matrixmaterials viz., storage. Ascurcuminisinsolubleinwater,two in lassi and ensuring its stability during a protocol forincorporation ofcurcumin etc. Thestudywasenvisagedtodevelop antidiabetic, hypotensive,hypocholesteremic, anti-Alzheimeric, antioxidant,anticarcogenic, functional attributes e.g.,anti-inflammatory, possesses numerousin Indianspiceturmeric, Curcumin, thebiologicallyactivepigmentfound Attribute againstAlzheimer’sDisease Geriatric DairyFoodhavingProphylactic efficiency. showed 100% stability and 99-97% encapsulation created forencapsulatingherbbioactiveswhich with dairyingredientsasmatrixmaterialswere of storageat80±1°C.Doublelayeremulsion –– DairyTechnology –– β-cyclodextrin, 93% retention of curcumin at 90-95% RH when β-cyclodextrin 6.03/ 100gwhenpackaged inpolystyrenecups. storage. Thecostofdeveloped productwasRs. dahi. in ofadhesion andreducedwheysyneresis work and firmness viscosity, increased texture, and addition oftransglutaminaseimprovedbody sensory and instrumental measurements. The firmer than the control the than firmer the texture.Theenzymetreatedproductwas used successfullyintheproducttoimprove treatment. Transglutaminaseenzymewas possible totalsolidslevelsmoderatedbyenzyme and reducedwheysyneresiswithminimum dahi The project aimed to produce good quality Texturization ofDahiforExtendedShelf-life Recommended DailyAllowance. of protein, 8% of dietary fibre and 2.5% of Vit-A of 14% approximately provides product of ml) (200 bottles) attheprevailingprices. One such serving at wasestimated ofdevelopedproduct The cost days and60at30°C40°C,respectively. remained acceptable evenafter90days at 4°C, 75 smoothie the revealedthat Shelf lifestudies contents were 31.46 mg/100 g and 240.65 IU/L. acid andvitaminA was formulated.Thephytic (SPI)andsucralose isolate protein sources, soy fruit (mango),vegetable(carrot),honey,milk on germinatedchickpeaflouralongwitha from carbohydrate.Abreakfastsmoothiebased increased protein and reduced calorie contents with RTDbreakfastsmoothie cereal based This projectaimedatformulatingadairy-fruit- Enhanced FunctionalAttributes Dairy-Fruit-Pulse BasedBreakfastSmoothiewith amylases, xylenasesandhemicellulases. an enzymebouquetconsistingofproteinases, of biscuits could beimproved withtheaid of free shortening.Thesensory qualities andtexture trans-fat inter-esterified by completely replaced shortening was concentration. Traditionalbakery resistant starch was incorporated inappropriate fibre, dietary increase to order In achieved. be fat with a polydextrose fat replacer Litesse could of replacement of level significant A sucralose. FOS- and maltitol of combination a by 100% germinated pearlmillet.Sugarwasreplaced and gram Bengal wheat, whole flours: three of multigrain flour composed of selected proportion dairy-cereal base.Biscuitsweremadefroma ` 19.43 per200mlserving(packagedinglass The productwasacceptable over21daysof in termsofimprovedbodyandtexture dahi as illustrated by at different time intervals and most significant most and intervals time different at cells ontreatmentwithLp91wasinvestigated and expression of incretins inSTC-1pGIPNeo of 33.42pMcouldberecorded.Bothsecretion that ofcontrolwhereinconstitutivesecretion GLP-1 secretionby101.31pMincomparisonto conditions, againheatkilledcellsofLp91elicited maximum secretion of GLP-1. Onsimulating gut the cellculturemediumwasoptimizedtoget observed withheatkilledpreparation.Further, muffin contained 17.43, 6.06, 1.62 and 50.58% and 1.62 6.06, 17.43, contained muffin eggless optimized The muffins. eggless calorie agent) wasusedfordevelopmentofreduced and sugar replacer(sucralose along withbulking proteins milk of Combination muffins. eggless developed for the manufacture of reduced calorie The formulationandprocesstechnologywas Eggless Muffins Process DevelopmentforReducedCalorie GLP-1/10 1 cellsrespectivelyto27.54,149.42and45pM cells elicitedthesecretionofGLP-1fromSTC- cellsandsonicated killed Lp91 viz.livecells,heat plantarum Lp91(Lp91).Differentpreparationsof fold level of expression of GIP (3.58±0.75) with secretion andexpressionaswellmaximum cells, usedinthisstudyinducedhighestGLP-1 against diabetes mellitus – 2: STC-1pGIPNeo a satietyinducinghormone,asprophylactics expression ofGlucagon likepeptide-1 (GLP-1), Screening probiotics and prebiotics for Health Bioactive CompoundsforImprovedHuman Foods with Prebiotics, Micronutrients and Other Research onNutraceuticalsfromMilk,Functional 6 cells/hrwithmaximumsecretion GLP-1 SecretionfromSTC-1pGIPNeo Cellsinducedwithprobioticlactobacilli D airy M L. L. icrobiology 37°C from an initial count of ~7.0 log cfu/ml. The ~9.5 log cfu/ml at stationeryphase after 12 h at counts reached viable culture volume).Thetotal L (2.5 scale fermenter and flask at both medium patterns ofthecultureswerestudiedinwhey from NCDC, NDRI. The growth and acidification Lactobacillus rhamnosus probiotics: Healthy foods:Productionofstableandactive in thefollowingFigure. able tostimulatethehighestsecretionasshown probiotic strains, heat killed form of Lp91 was live and heat killed preparations of indigenous Lactobacillus fermentumLf1( a timedependentmannerbybothLp91and to bemaximumat120minandincreasedin incubation. GLP-1 secretion was also observed 120minofco- expressionafter gene GIP preparations were effective in up-regulating similar conditions. Both live and heat killed also monitoredatdifferenttimeintervalsunder satiety inducing guthormone involved was expression. Similarly, expressionofGIP,another gene pro-glucagon (1.257)stimulated Lp91 where bothberberine(4.422)andheatkilled up-regulation was recorded at120min. duration, prepared withegg. muffins muffinscompared to calorie eggless The calorie content was reduced 21.08% in reduced were 383.45and347.06kcal/100g,respectively. eggless muffin and reduced calorie eggless muffin 1.63 and 53.53%, respectively. The calorific value of 6.01, 16.10, were muffins eggless calorie reduced content in ash andcarbohydrate protein, The fat, respectively. ashandcarbohydrate, protein, fat, Lactobacillus casei NCDC 610wereprocured Lf1). Ontestingthe NCDC 298 and

47 ndri annual report 2013-14 48 ndri annual report 2013-14 (mainly available probiotic bacteria: Status ofantibioticresistanceamongcommercially 12 to13logcfu/g. The viable cell counts in freeze dried powder were freeze drying. 10-11 logcfu/mlandpreservedby of range inthe cellconcentration viable get to milk washed andresuspendedinsodiumglutamate ~7.0 logcfu/ml.Thecellbiomasswasharvested, cfu/ml reachedafter10hfromaninitialcountof phase withtotalviablecounts ~9.5log Stationary L. rhamnosus NCDC were0.45and 4.8,respectively. specific growth rates (µ) of at 37°C.AtpH6.0and6.5, neutralization external under fermenterscale(5.0Lculturevolume)by NCDC 610inwheymediumwerealsostudied patterns ofL.caseiNCDC298andrhamnosus growth the Further, 0.42 and0.43,respectively. casei value of 6.5-6.7. The specific growth rates (µ) of pH ofmedia decreased to 3.8-4.0fromaninitial –– DairyMicrobiology –– found tobeingoodagreement. The brothmicrodilutionandE-testresultswere method. microdilution broth using by confirmed These resultsobtainedfromE-testwerefurther Commission, 2008 which is a matter of concern. to be higher than recommended by European wasfound isolates ofthe formost strips E-test array of antibiotics. The MIC as determined using Most of these isolates were resistant against a wide guidelines givenbyEuropeanCommission, 2008. antibiotics usingdisc diffusion method as per the different 50 against profile sensitivity antibiotic recovered organisms were screened for their characterized for antibiotic resistance. All the from 26commercialprobioticproductswere L. casei,fermentum,acidophilus)recovered Antibiotic susceptibilityofLactobacillus isolatesusingDisc NCDC 298andL.rhamnosus610were L. rhamnosus, diffusion method L. plantarum, L. caseiNCDC298and A total of 28 isolates L. reuteri, L. Antimicrobial activityofbioactive components 268, RhizopusoryzaeNCDC52 ) flavus NCDC NCDC 267,A.niger315, A. Candida spoilage microorganisms ( essential oilsagainst citral, trans-cinnamaldehyde and vanillinof carvacrol, thymol,eugenol,linalool,menthol, activity of plantbioactive components viz essential oilsindairyfoods: Preservation potential ofbioactive components of Lactobacillus rhmnosus(2)byspeciesspecificPCR. NCDC 49,Kluyveromyces cerevisiae Saccharomyces ATCC 25922, Pseudomonasaeruginosa MTCC 741, NCDC 211,E. faecalis NCDC223,Escherichiacoli Bacillus ATCC 15303, Staphylococcus aureus subsp. were alsoidentifiedasLactobacillus Lactococci of members the as mainly identified further were milk tubesbyvisualmethod.Theseselectedisolates production of higher amount of ropiness in skim selected basedontheformationofcapsuleand were cultures producing EPS prolific 48 these, biochemical andmoleculartechniques.Outof Streptococci andEnterococciasidentifiedby the membersofgeneraLactococci, as identified were isolates These samples. these EPS producinglacticcultureswereisolatedfrom (EPS) producinglacticacidbacteria.Atotalof205 Karnal Districtforisolationofexopolysaccharide well asfromhouseholdsofnearbyvillages Kurukshetra, Shahbad and Nilokheritownas collected from different dairies of Karnal, Ambala, lactic acidbacteria: Diversity of exopolysaccharides produced by inhibition ofα-glucosidaseusingyeastenzymes. The selected isolates were furtherscreened for the much less in the raw and fermented milk products. bamboo shoot,dahiandhumanmilk,whileitwas by followed producer, non-α-glucosidase be to Out of reach 30isolates of faecal origin were found identity ofalltheputativeisolatesaslactobacilli. the confirming PCR, specific genus by identified fermented products, 8). The isolates werefurther faeces, 26;dahi,10;rawmilk,20;bambooshoot,8; 5; milk, human (i.e. identification biochemical by confirmed were and method enrichment by or different dairyand plant sources by directplating total of77isolates for controllingpostprandialhyperglycemia:A alpha-amylase) inhibitoryprobioticlactobacilli Digestive enzymes(alpha-glucosidaseand bulgaricus Aspergillus niger NCDC44,Aspergillus guillermondi cereus NCDC66,Enterococcus and Lactobacilli (7) ,

Lactobacillus Milk andDahisampleswere lactobacilli food bornepathogensand S. cerevisiae NCDC42,S. . Afewlacticcultures marxianus NCDC Listeria monocytogenes antimicrobial In vitroantimicrobial were isolatedfrom acidophilus wasevaluated. MTCC 3160, Lactobacilli delbreuckii

faecium (6) and (6) and 46, ., ., ,

Lp75, LpS7, Both liveandheatkilledformsofLp9,Lp91, biomarkers inducedbyprobioticlactobacilli: –2 diabetes mellitus of analysis Expression inhibition againstabovepathogens. showed 12-16mmzoneof skim milkfermentate bioactive peptidesinskimmilkmedium.This toreleaseR29 strainswerefurthersubjected weak to moderate antimicrobial activity. R5 and aureus S. typhi,B.cereus, Salmonella ( antimicrobial activityagainstdifferentpathogens on milkagar.Theseculturesweresubjectedto activity byproducing>20mmzoneofclearance Thirteen cultures showed strong proteolytic activity asrevealedbydiscretezonesofclearance. proteolytic agar. Alltheculturesshowedvariable qualitatively byplatingonnutrientskimmilk sources were screenedfortheproteolyticactivity Lactobacillus cultureswereobtainedfromdifferent proteolytic lactobacilli:Ninetyfivedifferent bioactive peptides from milk proteins by antioxidant andantihypertensive Production of these isolatesaslactobacilli. all confirmed additionally primers specific genus further work.The molecular characterization using were presumptivelyconsidered aslactobacillifor of which137isolateswerecatalasenegativethat Out of206isolates,164wereGrampositive,out the selectiveisolationoflactobacillifromfaeces. vancomycin was foundtobethemostsuitablefor and bromocresol green media supplementedwith in successfulisolationoflactobacilli.TheLAMVAB be fairlywidespread,asallthesamplesresulted samples tested, lactobacilli isolates were found to were collectedfromdifferentlocations.Amongthe of rawmilk,cheese,dahi,lassi, kimchi, faeces, etc. obesity (DBTBioCARe):Atotalof125samples satiety hormonesinhuman cell lines to combat Mechanistic evaluationofprobioticsongut followed bycitralandeugenol. cinnamaldehyde asmosteffectiveantifungalagent inhibition of yeasts and molds indicated thattrans- and citralaspoorinhibitors.Theresultson concentrations andmenthol,vanillin,linalool, borne spoilage bacteriaandpathogensatlow and carvacrolasmostpotentinhibitorsoffood revealed thymol,trans-cinnamaldehyde, eugenol strains selected(Lp9,Lp91 and LpS7),Lp91 luciferase reporterassays. Amongstthethree RealTimePCR(RT-qPCR)andas revealedby TNF-α with conjunction in conditions treatment in Panc-1cellsbothunder postandco-culture %) (30-50 NF-κB of expression the regulated Shigella dysenteriae, E. coli, L.monocytogens, Lf1 significantly down significantly Lf1 L. fermentum ) which showed ) whichshowed CLA was noticed for eight lactobacilli. ThePCR CLA wasnoticedforeight of production higher significantly medium milk tested inskimmilkmedium.In were further ml inMRSandforacomparative study,20isolates range of19.5-76.4µg/ production ofCLAinthe In UV screening method, 61 isolates showed drugs. synthetic after obesity, treat to available CLA producer, as it is the most efficacious option sources andwerescreened togethight10,c12 of 285lactobacilli for controllingobesity in mouse model: A total Conjugated linoleic acid producing lactobacilli 41 byL.caseiandonlyGPR-40rhamnosus. isolates selected of presence in significantly regulated up expression of different free fatty acid receptors was using G-proteinandCD14inhibitors.Besidesthis, GLP-1 sincenoexpressionwasrecordedon and G-proteins mightplayaroleinexpression of regulated and CD14was overexpressed. CD14 down significantly was MyD88 level. significant a to regulated down were JNK) (NF-κB, factors Transcription and TNF-α) (IL-6, Interleukins the PCR array.Majorityof genes (84innumbers)by pathway signalling receptor panel ofTolllike was investigatedforexpressionprofilingofa rhamnosus and strains viz.L.acidophilus, hormones withLb-4,Lb-6alongthereference gut two expressionofthe maximum demonstrated determined byRealTimeQuantitativePCR as andGIP ofGLP-1 expression However, the as determined by RIA and ELISA respectively. reference strainsofL.johnsoniiand two with along Lb-3 wasrecordedwith GIP of DPP IV. Maximum secretion of GLP-1 and inhibition % 25 to Lb1-Lb4 andL.paracaseiled isolates the of four with treatment Similarly, %). significant alpha-glucosidaseinhibition(60-80 ) demonstrated rhamnosus strains (L.caseiand the 8, MCP-1andCOX-2inPanc-1cells.Majorityof IL- IL-1β, TNF-α, of regulation down with along expression ofSOD-2andGPx up regulation significant by revealed as potential anti-oxidative LpS7 exhibitedhigheranti-inflammatoryand Expression analysisofMouseTollLikeReceptorSignalling Lactobacillus i.e. GPR-41and120byLb-6,GPR-40 pathway usingPCRarray Bifidobacterium bifidum. L. rhamnosus isolates along withtworeference were isolatedfromdifferent L. casei, L. plantarum, –– DairyMicrobiology –– L. rhamnosus L.

49 ndri annual report 2013-14 50 ndri annual report 2013-14 three months, a reduction of 29.71% (P<0.005) in (P<0.005) 29.71% of reduction a months, three supplemented withmilkfermented byLg70for When ratswerefedwithcholesterol enricheddiet hypocholesterolemic and anti-oxidative potential. The isolateLg70wasalso evaluatedforits group. control to comparison in fluid intestinal milk showedhighconcentrationofIgAin control group. The mice that received fermented with milkfermentedLg70incomparisonto (P<0.005) increasedseveralfoldsinthemicefed weresignificantly activity enzyme lysosomal studies. Theperitonealmacrophagesactivityand mice modelwereusedforimmune-modulation hypocholesterolemic andanti-oxidativeeffect was evaluatedinratsforits functional property The invivobio- in vivobio-functionalattributes. L. gasseri DNase activityandwerefoundtobesafe.The for gelatinase,hemolysisand testing for safetyby 2 celllines.Theseisolateswerefurtherevaluated antibiotic resistance and invitroadhesion to Caco- assimilation, hydrophobicity, BSHactivity,invitrocholesterol for furtherin-vitroevaluationcellsurface the gastric pH of 2.0 and 2% bile and were selected and 2% bile), only nine strains were found to resist (1% concentrations bile and 6.5) and 3.0 (pH-1.5, infant faeces(0-3month’sage)todifferentacid L. gasseriisolatesrecoveredfrombreastfedhuman human infantfeces:Onsubjectingtwohundred probiotic Selection andcharacterizationofpotential control obesity. and UCP-2(1.6folds)inadiposetissuesgenesto folds) (1.8 FAS folds), (3.0 C/EBP-α folds), (2.0 positively altered the expression of SREBP-1c group comparedtoHFDcontrol.Moreover,it profiles, reduced the body weight in HFD treated glucose (~1.2folds), normalized the blood lipid lowered theplasmaleptin(~1.5folds) and blood significantly mice obese C57Bl/6j diet fat high to supplementation inSMcontainingderivedt10,c12, ml, pH 6.0andincubation 48h.Its progressive optimized by using the parameters viz.LA-0.5mg/ for animalstudies.HigherCLAproductionwas fermentum by GCMS.Basedontheprobioticproperties,L. reconfirmed was and SM in production CLA c12 ml). InGC,isolateDDHI15,showedhighert10, milk thanL.plantarumDDHI64inMRS(76.4µg/ DDHI15 (90.6 µg/ml) produced more CLA in skim and followed byL. gasseri (30%), isolates, 46% representing species predominant that revealed identification –– DairyMicrobiology –– L. salivarius (5%). Amongst these, isolate Lg70 was selected forfurther DDHI27 was selected as thebest culture Lactobacillus gasseristrainsfrom in vitroanti-oxidativepotential, L. plantarumasthe L. fermentum L. plantarum (20%) by PCR,doubledigestionofrecombinantvector confirmed were rS-NCFM and rS-Lp9 of clones NCFM forcloninginpET-22b(+).Recombinant sitesforfor Lp9;andNcoIXhoIrestriction designed withEcoRIandXhoIrestrictionsites protein ofL.plantarumWCFS1.Theprimerswere surface cell the to similarity sequence significant recorded. Oneofthebands sizing~56kDarevealed whereas in case of Lp9, four proteinbands were 48.5kDa onSDS-PAGEwasobtainedfromNCFM, by lithiumchlorideandasingleproteinbandof~ S-layer proteinfromLp9andNCFMwasextracted protein) fromprobioticLactobacillus spp.: Native recombinant surface-layer protein (S-layer Production andpurificationofnative functional dairy foods to combat life style diseases. candidate. Lg70 can be,thus, used informulation of gasseri strainLg70tobeapromisingprobiotic lipids. TheresultsofthepresentstudyshowL. the major organ confronting the external oxidized in comparisontocontrolgroupbecausetheliveris liver wasfoundtoincreaseinratsfedwithLg70 to control group. The activity oftheseenzymes in receiving milkfermentedwithLg70incomparison rats of erythrocytes the in decreased significantly peroxidase were oxide dismutaseandglutathione three anti-oxidativeenzymesviz.catalase,super oxidized soyoilforthreemonths.Theactivitiesof oxidative potentialratswerefedwiththermally feces of rats receivingLg70.To evaluate theanti- increased and coliforms decreased significantly in (P<0.005), respectively.Thenumberoflactobacilli 20.69% and (P<0.005) 49.54% was triglycerides TC wasobservedandthereductioninLDL-C Surface layerproteinfrom NCFM L. plantarum 9andL.acidophilus profile for the preparation of preparation the for profile tolerance, fast curd setting and good acidification NCDC 436wasselected based onitshighsugar for Defined strain direct vat set (DVS) starter culture further purifiedandrefolded. only asinsolubleinclusionbodies,whichcanbe induction. However,theproteinwasexpressed were made by reducing thetemperatureto22 were madeby For solubleexpression of protein,furtherefforts inclusion bodies in case of NCFMbutnotinLp9. the expression of~48.5kDarS-layerproteinas and 16 (0.1, 0.5, 1.0 and 5mM) and temperatures (37, 22 be recordedwithIPTGatanyoftheconcentrations However, theexpressionofproteincouldnot for expressionofproteinbyIPTGandlactose. S-layer proteintransformantswereinduced Lp9 andNCFM,respectively.Therecombinant recombinant genes with thenativegenes of both of similarity sequence 99.9% and 99.3% showed and nucleotidesequencing.Sequencingresults and 16 with lactoseat1.0and5.0mM37 and sensory characteristics. The quality of setting time,physico-chemical,microbiological of The suitabilityoftheculturesforpreparation dried cell pelletwas stored at -20ºCincryo-vials. dried powderwere11to12logcfu/gandfreeze by freeze drying. The viable cell counts in freeze the rangeof10-11logcfu/ml and was preserved protective medium)toachieveviablecellcountin resuspended in sodium glutamate milk (cryo- by harvestingandwashing.Thecellpelletwas broth bybatchculturing(42ºCfor6h)followed were preparedinwheybasedmedium and M17 broth. BiomassofNCDC436and428cultures whey basedmediumandM17 NCDC 428inboth performance ofNCDC436wascomparableto prepared usingconcentratedfreezedriedcultures was evaluated on the basis ofcurd basis onthe Misti doiwasevaluated Misti Doi:Outof19S.thermophiluscultures, o o C; and giving heat and coldshockbefore C; andgivingheat C) used.Ontheotherhand,induction Preparation ofMistiDoiinearthen pot Misti doi o . Growth C ledto Misti doi o C

hypocholesterolemic effects ofnativestrains Antimicrobial, immunomodulatory and mucinolytic activities. of theevaluatedisolatesexhibited haemolyticor None respectively. Norfloxacin to sensitivity and and BIF77,whichshowedintermediateresistance BIF7 except Vancomycin and Norfloxacin Acid, isolates demonstrated resistance to Nalidixic to seventeenofthetwentyantibioticstested.All and biletolerantisolateswerefoundsensitive acid the of Majority 30%). to (19 auto-aggregate xylene. Besides, allisolates showed the abilityto or n-hexadecane of presence the in 37% to 28 surface hydrophobicityofisolatesrangedfrom L. monocytogenes, S.typhi and B. cereus.The cell activity against common pathogens likeE. coli, the 10isolates showed discernible antibacterial taurocholate andsodium taurodeoxycholate. All all of these were able to deconjugate sodium of sodium tauroglycocholate. Nevertheless, in caseofanytheisolatespresence BIF7 andBIF20),noprecipitationwasobserved hydrolase activity. Except threeisolates (BIF2, good survivabilitywereevaluatedforbilesalt showing isolates 10 which in oxgall, 2.0% to 15 strainswerefurtherscreenedfortolerance these of Out 37°C. at broth MRS acidified in h 3 for 2.0 pH at grew isolates fifteen which of h, 3 to surviveatpH2.5for seven isolateswereable desirable traitsandtheirsafetystatus.Twenty- system ofinvitroanalyses,whichincluded different speciesweresubjectedtoasubtractive human anddairysources, belongingtothree sources: lactobacilli isolatedfromhumananddairy In vitroscreening ofprobiotic properties of be usedasDVS/DVI. was comparable tothatof fresh cultures and can Freeze-dried DVSculture(Mistidoiculture) A totalof31lactobacilliisolatesfrom –– DairyMicrobiology ––

51 ndri annual report 2013-14 52 ndri annual report 2013-14 S. its immunomodulationpropertyonchallengewith capacity shown by 10 kDapeptidefragmentsof capacity shownby to beappreciablewithhighest radicalscavenging Antioxidative activity ofallthecultures was found from 6.9 to 2.9 or 3 after 48 h of incubation at 37 48 hofincubation from 6.9to2.9or3after all the cultures in soy milk decreased significantly increased from5.7to10.4logcfu/ml.ThepHof of incubationandwithL.helveticus increased from 5.9 to10.29logcfu/ml after 48h Lactobacillus strains(L.plantarumC fermentation ofsoybasedmilkbylactobacilli: Production ofbioactivepeptidesthrough Hence, adhesionandscanningelectronmicroscopy. per cent compared to RSI-3 as revealed by adhesion score, also foundtohavebetteradheringcapabilityas displayed betterantimicrobialactivity.RS-2was NCDC 113andL. and Gram-positivepathogenicbacteriaviz.S. invariably inhibited the growth of Gram-negative by and furtherfortheirimmunomodulatoryactivities salt hydrolase (Bsh) activities byin vitro Caco-2 celllines,cholesterolassimilation,andbile were evaluatedfortheirantimicrobial,adhesionon rhamnosus RSI-3withL of nativeoriginnamelyL of probioticlactobacilli:Twostrains –– DairyMicrobiology –– ml/min) andL.plantarumC rhamnosus ml/min), followed byL.rhamnosus C NCDC 288 exhibitinghighest activity (13.20 nmol/ ability to produce α–galactosidase with cultures usedinthepresentstudydemonstrated membranes. TotalcellcountofL.plantarumC fragments werepreparedusingMWCO peptide using allthecultures separately and 10, 5and 3kDa 288. Fermentationofsoyderivedmilkwasdone NCDC a referencestrainofLactobacillushelveticus were identifiedby 16sRNA sequencing along with L. plantarum C and acidityofsoymilkduringfermentationwith typhi andL. method. Although, all the three strains in vivomethod.Although,allthethreestrains L . RS2 was selected for assessing fermentum RS2wasselectedforassessing C 8 , C 2 increased from 0.117 to 1.8%. All the 25 monocytogenes usingmicemodel. ,

C ATCC 53135, RS-2 monocytogenes ATCC53135,RS-2 28 , and C Adhesion ofL.fermentumRS-2,onCaco-2celllineasstudiedbySEM . GG as control rhamnosus GGascontrol . 2 34 fermentum RS-2andL (12.50 nmol/ml/min). (12.50 nmol/ml/min). ) isolated from cheese ) isolatedfromcheese 8 NCDC 288,it

(12.57 nmol/ 2 L. helveticus L. helveticus and C methods methods typhi 6 , o L. L. C 2 .

followed by RSI-3 and RS-2. followed byRSI-3andRS-2. to deconjugateconjugatedbilewashigherinLGG efficiency The biles. mixed to compared bile single indicated that substrate specificity was more towards of glycineliberatedfromconjugatedbilesalts, amount the by quantified as activity, BSH RS-2. to compared as RSI-3 by efficiently more hydrolyzed as comparedtoRSI-3,whileGCAandTDCAwere grow and hydrolysesodium taurocholate washigher to RS-2 of efficiency The respectively. 51.85% and RSI-3, and LGG was observed to be 59.36%, 29.44%, Cholesterol assimilationabilityofprobioticRS-2, of pathogensinspleen,liver,andlargeintestine. Probiotics also significantly reduced the colonization concentration amongthedifferentgroupsofmice. IgG and IgA, in observed was difference significant with animprovedimmunologicalstatusbutno Feeding ofprobioticswasfoundtobeassociated plantarum C milk wasfermentedusingLactobacillus when soy HHL substrate and 62.5% inhibition was observed activity offermentedsoymilkwastestedusing L. rhamnosusC 288 NCDC helveticus L. L. plantarum C cholesterol assimilation activity (49.76±0.21%) activity assimilation cholesterol broth. Similarly,L.rhamnosus GG showedhighest followed byL.delbreuckii probiotic countsinpresenceof theaboveprebiotics highest exhibited GG and cream.L.rhamnosus delbreuckii LT-4, bulgaricus-2, L.rhamnosusGG(LGG),plantarum of six maltodextrin) oncholesterolassimilationpotential butter: Development ofreduced cholesterolprobiotic with and inhibition wasmeasuredagainstE.coli(29mm) between 8to29mmindia.Ahighestzoneof of indicatororganismstestedranged of inhibition (28 mm) when soy milk was fermented S. typhi(28mm)whensoymilkwasfermented L. plantarumC L. plantarum Lactobacillus strains In vitroeffectofprebiotics(inulinand 11 were evaluated in broth ssp. bulgaricus-11wereevaluatedinbroth 2. 2 25 (911.224 TEAC µM), followed by (874.695TEACµM).Thezones LT-7, L. plantarum 2 . In vitroantihypertensive (884.757 TEAC µM) and viz. ssp. bulgaricus- 11 in , L. delbreuckii L. LT-9 and L. ssp.

in cream. compared toL.rhamnosusGGandotherlactobacilli bulgaricus-11 resultedinexuberantgrowth24has in 24h.Ontheother hand, by in brothsupplementedwithinulinfollowed 1200 lacticacidbacterialisolates, only55isolates of number of a total : Out release controlled Micro-encapsulation of bacteriocins for their Mechanized Manufacturing System Biotechnological Interventions, Packagingand through Application ofNewProcesses, Value Addition to Traditional Milk Products time ofexposure. different forthesamestressagentdependingon susceptibility of lactobacilli were substantially kanamycin andgentamycin.Changesinantibiotic against ampicillin,chloramphenicol,clindamycin, results, asitledsixoftheisolatestobecomeresistant selected isolates but hightemperature showed better were significant in changing the sensitivity pattern of and hightemperaturewascarriedout.Allthestresses antibiotic sensitivity using low pH, bile salt tolerance resistant with ciprofloxacin. Assessment of stress on linezolid and vancomycin) but all the isolates were gentamycin, kanamycin,streptomycin,vancomycin, (viz. ampicillin,chloramphenicol,erythromycin, sensitive againstmajorityoftestedantibiotics different antibiotics.Nineisolateswerestrictly taken forstudying thesensitivity patternagainst 2 L.caseiand3salivarius.Finally11isolateswere at specieslevel.Outofthese,36wereL.plantarum, using genus specific PCR that were further identified lactobacilli as confirmed were 41 samples, based Amongst a number of isolates from different dairy lactobacilli fromtraditionaldairyproducts: Influence ofstressonantibioticresistancein control, respectively. to be 21.07±0.64 and 17.08±0.83% for test sample and 42˚C/24 h. Cholesterol reduction in butter was found 3% and ripened with cream (35% fat) supplemented with maltodextrin @ with reducedcholesterol.Butterwaspreparedfrom reduction increamforpreparationofprobioticbutter and maltodextrinwereselectedforcholesterol (16.76±0.14%). Finally, control to compared as h 42˚C/24 at bulgaricus-11 observed with 3% maltodextrin by was %) (23.44±0.11 reduction cholesterol Highest in cream for L. delbreuckii ssp. bulgaricus-11 and LGG. were found to be optimum for cholesterol reduction of 3% and incubation temperature of 37˚C and 42˚C and 11.8±0.21%, respectively in cream. Inoculum size bulgaricus-11 andL.rhamnosusGGwas17.28±0.16 L. delbreuckii ssp.bulgaricus-11(47.67±0.19)L. delbreuckii Cholesterol reductionbyL.delbreuckiissp. -11 at L. delbreuckii ssp. bulgaricus-11 at 11 L. delbreuckiissp.bulgaricus-11 L. delbreuckii ssp. L. delbreuckiissp.

sp., two strains were found strains ofKluyveromycessp.,twowerefound evaluated spectrophotometerically. Outoftotal10 also was potential production β-galactosidase strains 6C17and6C18.Inaddition,theirwith least residuallactoseconcentration wasobserved assaying residualsugaratdifferent timeintervals, on Simultaneously, medium. lactose 5% in (v/v) 3% ~ be to observed was production ethanol maximum the levels, sugar different at profiling Further, basedontheirethanolproduction were found to be the highestbiomass producers. showed good growth and strains 6C17 and 6C18 were identified as strains putative the All lactose. % 25 to tolerant were isolates total of 8% whereas 10% to up tolerance ethanol showed isolates the of 9% than be recoveredfromdifferentdairyproducts.More whey: Atotalof200isolatesyeastcould from tolerant andmetabolicallyengineeredyeast Whey tobiofuel:bioethanolproductionbystress important antibiotics. sensitive toclinically was foundtobe C63 isolate haemolysis, gelatin liquefaction andDNAse. The K. pneumoniae.C63isolategavenegativetestfor encapsulated bacteriocinwerestudiedagainst Release kineticsofmicroencapsulatedandun- Sodium alginate (2% w/v) + chitosan (0.4% w/v). phosphatidylcholine, + w/v) (0.4% guargum + w/v) (2% alginate Sodium w/v), (0.4% guargum + w/v) (2% alginate Sodium w/v), (2% alginate different wallmaterials(20:80v/v):Sodium CFCS of C63was microencapsulated infour may, thus,bedesignatedasaglycoprotein.The non-proteolytic enzyme (amylase). The bacteriocin found tobesensitivealltheproteolyticanda weight ofabout4-5kDa.Thebacteriocinwas revealed thatthebacteriocinhasamolecular activity. TheSDS-PAGEandin-situ bacteriocin the of recovery 82% yielded which purified by 0-60% ammonium sulphate saturation, at pH6.5and7.BacteriocinfromC63waspartially was observedinMRS broth after22hincubation for furtherstudies.Maximumbacteriocinactivity Enterobacter, Klebsiella,Serratiaandwasselected including C63, however,showedbroadspectrumofinhibition lactis sp.lactis an isolateLactococcus produced by and activeoverawiderangeofpH.Bacteriocin stable heat Bacteriocins ofthesestrainswerequite genus tothe belonged Fiveisolates spoilage bacteria. well asGram-negativefood-bornepathogensand activity againstsomeoftheGram-positiveas them, only8isolatesshowedgoodantimicrobial were foundtobebacteriocinogenicandamongst Lactobacillus andthreeisolatestoLactococcus. E. coli, Yersinia, Citrobacter, Proteus, sp. All the strains Kluyveromyces sp.Allthestrains –– DairyMicrobiology –– gel activity

53 ndri annual report 2013-14 54 ndri annual report 2013-14 48) procured from Delhi and UP area were total of131milksamples(raw83andpasteurized Survey work on antibiotic residues in milk: A of milksampleinLSEMfollowedbyobtaining 0.15h. Theassayprotocolrequirespre-enrichment selective enrichment medium (LSEB) for 16.0 ± pre-enrichment oftargetedsample(s)inListeria ± 0.8 withinrealtimeof 4.0 ±0.30hafterinitial detection ofL.monocytogeneswithlogcells1.74 substrate hasbeendeveloped which enablesthe using markerenzymesandtheirgerminogenic on96microwellplate detection ofL.monocytogenes contamination inmilk:Asporebasedassayfor bacterial and metals heavy aflatoxin, residues, and micro-techniquesfor analysisofpesticide NAIP projectonDevelopmentof biosensors Biotechnological Concepts through ApplicationofNewerChemicaland Ensuring QualityControl Generation Toolsfor Health ConcernsandDevelopmentofNew Clean MilkProduction with aFocusonEmerging units ofβ-galactosidase. 6C17 and6C18produced ~ 1140and830miller producer with ~ 988 miller units. On the other hand hyper β-galactosidase found was 1389 MTCC strain and whey in production β-galactosidase (MTCC, Chandigarh)werealsoevaluatedfor procured fromMicrobialTypeCultureCollection and 6C18.FivestrainsofKluyveromyces marxianus 6C17 namely producers β-galactosidase hyper –– DairyMicrobiology –– Total %incidenceofAflatoxinM1inyear Incidence ofantibioticresiduesinmilk samplescollected Note: CAP:chloramphenicol;Tetra: Tetracycline 2013-14 byRosareader during 2013-14 Total %incidenceofAflatoxinM1in Raw milksamplesbyRosareader for the presence of Aflatoxin M procured from Delhi andUP area wereanalyzed 131 milksamples(raw83andpasteurized48) was evaluatedforitsantibiotic sensitivityunder its prototypewasdeveloped. Thekitprototype selected strain was optimized and subsequently, substrate was selected. Thespore productionof specific its and (s) enzyme marker for screened sensitive strainofBacillussterothermophillus was detection ofantibiotic residues in milk: The most for basedsensor/assay ofspore Development antibiotics. different for codex by specified dose MRL at % collected during the year showed incidence of 9.16 during theperiod from 2013-2014.Milksamples analyzed forthepresenceofantibioticresidues Survey work of aflatoxin M aflatoxin of work Survey Residues andEnvironmentalContaminants” Outreach Projecton“MonitoringofDrug and pasteurizedmilksamples. been validated in the labwithspiked/naturalraw interfere intheassay.Thesporebasedassayhas S. aureus, incubation. ThecontaminantslikeEnterococci, The assayscandetectupto5logcellsin4hof plate reader.wavelength usingmicrobiological excitation/emission specific at read be to is plate spore germinationinwellplate.The96 of detection for added is substrate fluorogenic is thenincubatedfor4hat37°C.Afterincubation, and dormantspores in a96wellplate.Theplate incubated withdifferentgerminogenicsubstrates enriched cells. The enrichedcells obtainedare milk samples was observed as 10.63 % and 2.12 %. the otherhand,sameincidenceinpasteurized milk samples was found positive at ≥ 0.25 ppb. On 5.95 at ≥ 0.5 ppb was observed and none of the raw observed. Inrawmilksamples,atotalincidenceof was ppb 0.25 at % 3.81 and ppb 0.5 ≥ at % 4.58 of and AOAC approved charm assay. Anincidence otherthanListeriaspp.donotE. coli pasteurized milksamplesbyRosareader Total %incidenceofAflatoxinM1in 1 inmilk:Atotalof 1 using Rosa reader usingRosareader detection stage enzymeassay’’wasdevelopedforthe detection of Development ofenzymesubstrateassayfor and presenceofantibioticresiduesinmilk. color development indicates inhibition ofspores residues inmilk.Noyellow absence ofantibiotic and its interaction with substrates depicts the germination and release of specific marker enzyme The appearance of yellow color indicates spore followed byincubationat64±2ºC for2.00-2.15min. with 200±20µlofgerminationmedium. This is (Stage II)containing100±10µlsporesseeded content fromstage-Iwastransferredintoampoule and mixedwell.Theentire chromogenic substrate ±10 µlofmilksamplewasaddedtolyophilized germination medium(stage-II).InstageI,100 lyophilized (stage-I)andsporeswereseededin stage Iand II wherein chromogenic substrate was field conditions. The optimized procedure includes in was developedforthedetection ofE.coliin milk products: biosensor formonitoring E. coliinmilkand Development of enzyme substrate based collected fromdifferentsources. and pasteurizedmilkaswellcheesesamples lab withspikedreconstitutedmilkandfewraw reference method.Theassaywasvalidatedinthe and demonstratedparallelresultswithstandard of foodsamplesinLSEMfor25±1.0h(Stage-I) of 4.30±0.30h (Stage-II) afterinitialpre-enrichment the presence of L.monocytogeneswithinrealtime log cfu). The two stage enzyme assay can confirm E. aerogenes(6.52logcfu)andK.pneumoniae(7.32 E. coli(7.34logcfu),Salmonella abony (7.43logcfu), L. fermentum(7.54logcfu),B.cereus cfu), against significantly medium (LSEM) wasfoundtobeincreased modified medium i.e. Listeria selective enrichment 0.05%, respectively. The antimicrobial spectrum of of esculin and ferric ammonium citrate @of 0.1 and developed intoachromogenicmediumbyaddition and modified further was contaminants potential selectivity interms of its inhibition patternagainst respectively. TheFraserbrothhavinghigher University ofVermontMedium andFraserbroth, in 46.28% and 62.5% by followed (64.28%) Broth log count was observed inListeria Enrichment in increase significant a and media commercial L. monocytogenes was studied inthreedifferent by colourchange.Initially,growthpatternof free chromogen that can be visually detected release to (s) enzyme marker specific for reaction “enzyme-substratethe principleoftargeting L. casei (7.32logcfu), S. aureus(5.38logcfu),L. of L. monocytogenesinmilk:A‘Two- L. monocytogenes An “Enzyme (s) substrate assay’’ Enterococcus faecalis (7.15log Enterococcus in milkbasedon (6.45logcfu), as concentrationofdifferent sugarsasgerminant uptake principle.Anarrayof assay parameterssuch were usedasmarkersugars fordevelopmentof germinants selected and modified was protocol germinogenic substrate(s). Subsequently, the assay coli enzyme(s), however,itdidnotworkwellwithE. developed successfullywithpuremarkers spores. Spore germination based concept was to actasagerminant forB.megaterium capability compound like glucosamine demonstrated their glucoside, sucrose,maltoseandnitrogenous α-methyl-D- β-gentiobiose, xylose, mannose, sugars likedextrose,lactose,fructose,cellobiose, B. megaterium (spores/cells) and enzymes was carried out in different strains of a comprehensivescreeningofthesemarker Initially, amino-peptidase. and α-D-mannosidase glucuronidase, β-D-glucosidase, α-D-glucosidase, β-D- α-D-galactosidase, β-D-galactosidase, of specificmarkerenzyme(s)whichincluded use ofselectedmarkersugarsbesidesaset for and markerenzymes: The developedassay bioassay forE.coliusinggerminogenicsubstrate Development of sporegerminationbased compared withexistingpriorart. or detectionofE for selectiveenumeration cost effectivesubstitute features ofhighsensitivity/selectivitywouldbea non-faecal contamination.TheECSM with novel presence ofE.coli,whileyellowcolorindicates the confirmed enzymes/substrates marker with Appearance ofgreencolorinECSM supplemented optimized forthedevelopmentofenzymeassay. of enzymes along with their substrates were contaminants werescreenedandformulation Different markerenzyme(s)forE.coliandother their concentrationvaryingfrom10to30mM. with β-D-glucopyranoside and β-D-glucoside α-D-glucoside, α-D-galactoside, β-D-glucuronide, medium byadditionofdifferentsubstrateslike developed intoachromogenic ECSM wasfurther casei (5.32logcfu). fermentum (5.54logcfu)andL. E. faecalis (4.19 log cfu), (4.28 log cfu), Serretia marcescens (4.63log cfu), cfu), against significantly increased E. coliselectivemedium(ECSM) wasfoundtobe i.e. medium modified of spectrum antimicrobial The agents. selective different with modified was medium optimized basedonlogcountsandthe extract, lactoseandpotassiumchloridewere growth supplementsnamelypeptone,yeast milk andproducts.Theconcentrationsof on accountofbeinguptake of sugarsused as E. coli/E.coliO157:H7inmilkinvolvedthe Shigella flexneri (4.35logcfu),Y. enterocolitica Shigella . coli within 12±0.25hwhen B. cereus S. abony(6.23log (4.45 logcfu), E. coli. –– DairyMicrobiology –– Different L.

55 ndri annual report 2013-14 56 ndri annual report 2013-14 37 (20 µL/6.65±0.25logcfu),incubationtime(3.00hat (100 mM - 300 mM), volume of spore suspension –– DairyMicrobiology –– in LSEM. color reactionafter16.0±0.30 hrsofincubation bioMérieux system. Three samples showed black ISO11290-1:1996 horizontal method and VIDAS® was performedinnatural milksystemwith using EMCCD system.Validationof theassay onIODand measurementwasdonebased micro well by adding 0.5 µl of reaction mixture assay wasfurtherminiaturizedonbiochip time of 1.0±0.14 log cells in Novel LSEM. The it wasachievedwith16±0.30hrsenrichment and 30minwhereasincaseofmilksystem, buffer was found to be 5.0±0.5log count in 4h respectively. TheLODofdevelopedassayin glycogen, esculin and L-alanine β-napthylamide glucopyranoside, methyl α-D mannopyranoside, β-D- methyl substrates germinogenic for fold 24.43±5.48, 25.82±2.83,36.68±8.48and0.79±0.02 having agerminationsignalviz.21.52±2.92, into 96wellplatemicrotitresporebasedassay optimized forthetransformationofassay were β-napthylamide L-alanine and esculin 37°C, concentrationofgerminogenicsubstrates, and incubationtimeforsporegerminationat monocytogenes for developmentofsporebasedassay.The L. monocytogenes suspensionandincubationtime level requirement, concentration, volume of experimental parameters such as heated spore substrates wereoptimized individually for min quiteappreciably.Theselectedgerminogenic to germinate B.megaterium D-dextrose, D-mannoseandL-alaninewereable germinant and germinogenic substrate screened effect onsporegerminationsignal.Amongthe of the specific marker enzymes to have an additive biosensing agents, were screened for thepresence sensors: monocytogenes inmilkusingsporebased An innovativeapproachfordetectionofL. on IODvaluesusingEMCCDsystem. was donebased reaction mixtureandmeasurement further miniaturized on biochip byadding 0.5µlof signalswereachieved.The assaywas and positive evaluated with milk samples under field conditions andalso contaminants positive andGram negative was screenedforitsselectivitywithdifferentGram selective medium (EC-SM) at 37 (5.00±0.30 h) of pure cells/spiked milk in with detectiontimeof3.00hafterpre-enrichment of sporebasedassaywas5.24±0.25logcount 7.02±0.21 logcfuwereoptimized.Thesensitivity o C) and inoculum level of target bacteria (20µL/ Bacillus megaterium2949sporesusedas cell suspension, spore suspension 2949spores within30 o C. The bioassay E. coli L. L. 10 h of incubation at 37 at 10 hofincubation Enterococci andshowedblackcolorafter17 was spiked @1.36and 3.32logcounts/mL of were alsoevaluatedatM/sSGSwhereinmilk (r~0.99). Thedevelopedmedia pasteurized milk a significantcorrelationinraw(r~0.975)and of incubation.Thecomparativecountsrevealed in 48h results wereobtained but similar pattern, solid conventionalmediai.e.CAAdemonstrated on the countstaken 9-11 hrespectively.Although, and 3-4log/mL at around18-24,14-18,11-14and were observed to beintherangeof0-1,1-2,2-3 on ß–D-glucosidaseactivity,enterococcicounts samples analysedforEnterococcibyEBSAMbased Among160 andfunctionalizedbiochip: media inmilkusingdeveloped Enterococci Screening of monocytogenes. ISO-11290 part-11996methodfordetectionofL. products and similar results were observed with assay wasvalidated indifferentmilk and milk performance ofthedevelopedtwostageenzyme developed twostageenzymeassay.Working of detection confirmatory time wasrecommendedforpresumptiveand milk, cheese,milkpowderanalysis,theincubation 1996 method.Based on rawmilkandpasteurized “two stage enzyme assay” orISO 11290-part-1 monocytogenes samples showedthepresenceofListeria None of thepasteurized butterand dried milk of presence the confirming not no colorwasdevelopedinESM(stage-2),hence showed blackcolorinLSEM(stage-1)andwhile raw milk,icecreamandcheese,3samplesofpaneer as byAPI Listeria developed method, 11290-part-1 1996and as well were found to bepositive for milk samples.Outof95samples,foursamples and QualityAssurancelaboratorywithspiked 28±1.0h andwasvalidatedinFood Safety within products coulddetectL.monocytogenesinmilk for detectionofL.monocytogenesinmilkand products: detection of L. in monocytogenes milk and milk Evaluation of“Twostage enzyme assay for was measured using microbiological plate reader. was measuredusingmicrobiological plate fluorescence and mixture reaction of µl 90 with well platesbyadding30µlof heatedsporesalong detection of Enterococci was optimized on 96 for assay based Sporegermination respectively. pathogens, yeastandmoulds and Bacilluscereus, log counts/mLofLAB,Gram–veandGram+ve restricted uptoof8.15,7.607.335.76,3.50 be with different contaminants and their growth could screening by was alsoevaluatedforitsselectivity “Two-stage enzymeassay” when analyzedbythedeveloped kit, respectively. Three samples of kit,respectively. Three samples of o C . The developed medium C .Thedevelopedmedium L. monocytogenesinthe L. monocytogenes by L. monocytogenes. developed spp./L.

sakazakii by formation biofilm of Efficacy counts ofEnterococci. increase in IOD and sensitivity could go up to 1-log significant a indicated data available The system. based onchangeindensityandIODusingEMCCD of reactionmixtureand measurement was done adding 0.5µl well functionalizedwithsporesby Assay wasoptimizedfurtheronbiochipmicro 20.00foldwas observed. germination from13.54to was further modified and a significant increase in 6.11, 1.61and0.75fold,respectively.Theprotocol of Enterococcii.e.6.6,5.4,4.8and3.6was13.54, The germinationofsporesatdifferentlogcounts stage SSHE,thevariation incapacitywas milk: khoa makingmachineforhandling concentrated Development ofscaleupdesign ofcontinuous Mechanized ManufacturingSystem Biotechnological Interventions,Packagingand through Application ofNewProcesses, Value Addition to Traditional Milk Products – DairyMicrobes:Twohundredandforty Network project on Veterinary Type Cultures Resources Utilization anduseofNon-conventionalFeed Nutrient Efficient Strategies, Feeding Improved Animals through Dairy of Raising Productivity a potentialsourceoffatalinfectionE.sakazakii purpose offeedingtoneonatescanbe used forthe and environmentalmilieu,thefeedingtubes indicated that E. sakazakii as comparedtostainlesssteelcoupons.Thestudy sakazakii by formation biofilm the that stated be can It to 2 it wasobservedthatthecellnumberincreased up sakazakii were used to check the ability and efficacy of efficacy and ability the check to used were stainless steelcoupons and enteralfeedingtubes haemagglutination andDNaseproduction).The for the virulent characteristics (i.e. hemolysis, E. sakazakii.Theseisolateswerefoundpositive as identified were 11 whereas primers, specific species using PCR with identification molecular The positive isolates (27)wereconfirmed by for Gram’sreaction,catalaseandoxidasetest. on tryptic soy agarand werefurtherscreened sources were selected based on the growth nd day,but,thereafter,cells started todislodge. In ordertoincreasethecapacity ofthree wasmoreincaseofenteralfeedingtubes for biofilm formation up to 10 days and days 10 to up formation biofilm for : A totalof45E.sakazakii can be present in dairy from different Enterobacter D airy E E. E. .

ngineering fixed at 1 kg/cm steam pressureinthirdstage SSHEwaskept 4-6 kg/cm of range the in kept was stage second and first of pressure steam and TS 30-50% of range the pressure. The milk concentration was varied in studied byvarying milk concentration and steam rpm, respectively.Itwasobserved thatcapacity 15 and 200,175 as fixed kept was stage third and CMCase andavicelase. xylanase productionwashighest,followedby production. Among different enzyme activities, and laterOrpinomycessp.NFRI-1dominatedthe 24 hfollowed by Orpinomyces sp.NFRI-13at48h xylanase, initiallyAnaeromyces enzyme activitythroughouttheincubation.For Anaeromyces incubation. Ontheotherhand,incaseofavicelase with respecttothestageofgrowthortime own characteristic pattern of enzyme production and 72h.Thisindicatesthateverystrainhasits that showedmaximumenzymeactivityat48 Orpinomyces andlater NFRI-6 sp. after 96 h. However, after 24 h, itwas was shownby activity (polyflagellated zoospores).HighestCMCase zoospores) andrestfourasOrpinomycesspp. were identified as isolates four patterns, flagellation zoospore and presence ofconstricted hyphae,pointed sporangia produce sporangiaoneachrhizoid.Basedthe (8-12 mm), as these possess nucleated rhizoids and ‘crossbred cattlewerepolycentricandlargerinsize isolatedfrom‘KaranFries fungi Anaerobic Veterinary TypeCulture–RumenMicrobes: products withimprovedtechnologicalqualities. dairy ofvariousfermented for manufacturing etc. were evaluated and the culturescan be used proteolysis, exo-polysaccharides (EPS) production, production, flavour profiles, acidification as such VTCC culturebank.Thetechnologicalproperties freeze dried ampoules and deposited at NCDC/ and Lactococcus spp.,LeuconostocLactobacillusspp. of 58 lactic acid bacteria comprising strains of Pradesh, Punjab, HaryanaandRajasthan.A total collected from different parts of Himachal samples products fromfermentedmilk isolated spp., isolates ofStreptococcusthermophilus, Streptococcus Lactobacillus spp. and 2 and2-3kg/cm sp. NFRI-4 exhibited maximum exhibited sp. NFRI-4 2 . The rotor speed of first, second Anaeromyces Anaeromyces thermophilus were preserved as Orpinomyces sp.NFRI-13 Leuconostoc 2 respectively. The respectively. spp. (uniflagellated sp NFRI-6after sp.NFRI-1 Anaeromyces Lactococcus spp.were

57 ndri annual report 2013-14 58 ndri annual report 2013-14 pressure in first stage-6 kg/cm stage-6 first in pressure (steam kg/h 180 to TS) concentration-30% feed 3 kg/cm sample householdsfromsecondary sourcewere veterinary services in India. About 29,020 dairy factors thatdetermined thelikelihood ofusing The study has shown theimportance of identifying Factors DeterminingtheVeterinary Services smallholder incomeandemployment. on thecapacityofdairyfarmingtocontribution development and reduction of poverty will depend poverty andinequality.Promotionofeconomic income distribution, buthelps inreducing absolute through inclusivedairyingdoesnotworsen growth that confirms also It inequality. income crop. The income from dairy farmingreduces the compared todistributionofincomesthrough through dairying and other livestock farming the hypothesisofrelativeincomeequalisingeffect farm households, which otherwise corroborates distribution oftotalincomeforallcategories and livestocksource has aequalisingeffectonthe other hand,theincomefromofffarm,dairyfarm caveat thatother things are unchanged. Onthe a with 1.38% by inequality income total trigger would income crop in increase incremental 1% alleviation programmes. The study revealed that level anditshouldbeanintegralpartofpoverty has capacitytoreduce poverty atthehousehold related to land ownership. Therefore, dairying family income of thehouseholdsisalso inversely ownership whereas as income from dairying to total the householdlevelisinverselyrelatedtoland on dairyingtototalproductionexpenditureat The studyrevealedthattheproportionalexpenses Development Process Inclusion ofSmallholdersintheDairy first stage-4kg/cm first was increasedfrom120kg/h(steampressurein the basis of ISO guidelines for Graphic technology. the basis of ISOguidelinesforGraphictechnology. Machine vision system (MVS) was designed on dairy productsbasedoncolourmeasurement: Machine vision system for qualityevaluation of system wasestimatedtobearound3500kg/day and half hour cleaning time, the capacity of the plant wouldrunforthreeshiftsinadaywithone texture (8.0to9.0).Thuswithanassumptionthat observed as color (4.0 to 4.5), alsoflavor (7.5 were to 8.5) products and final the of scores sensory 2 and feed concentration 50% TS). The TS). 50% concentration feed and 2 D , secondstage-2kg/cm airy E conomics 2 , secondstage- , S 2 and tatistics has threemodulesfordatabase management crossbred cowsandbuffaloes. Thesoftware of economicsmilkproduction oflocalcattle, (MILKEcAP-1.0) was developed for the estimation Production Economic Analysis Package Web-enabled user friendly software: Milk Various ProductionSystems Standardised MethodologyandEstimatesfor Costs and Returns in Milk Production: Developing explanatory variables. services ornotafterincorporatingthevalueof whether householdwillbeusingtheveterinary empirical point of view is very important to predict marginalised dairy farmers. The model from the latestanimalhealthcaretechnologiesto suitable organisationalchangestodisseminate could becomemoreeffective,iftheyundertake The institutionswhichsupportveterinaryservices in theIndiansmallholder dairy farmingsystem. agriculture rathereconomic grounds particularly was justified onsocialandclimate resilient government involvementinveterinaryservices activities that optimise productivity gain.Direct linked tootherinnovationsandmarket-oriented dairy value chains, are sustainably enhanced when one elementof only services, being veterinary the farming systemmode.Thestudypointedoutthat integrated inthe treated shouldbe it manner rather that dairysectorshouldnotlookinisolated use veterinary services once again and reinforced agriculture positively influenced the likelihood to services. Principal source ofincomefrom veterinary positive relationshipbetweenherdsizeanduseof selected fortheanalysisinthisstudy.Therewasa illumination. used. ThesystemwascalibratedforD65standard For largesizeproducts,windowportcanbe designed forsmallersizedairyandfoodsamples. measurement. Product loading platform was for loadingofproductintothesystemcolour product onparallelplanes.Provisionwasmade with camera at aninclinationb.Light-cameraand geometries: a.Light and product on parallelplanes can beoperatedusinganyofthefollowingviewing architecture usingWindow 7 (32bit).The system acquisition and analysis unit was based on 64 bit computer, control unit,etc.wereintegrated.Data components ofthesystemsuchascamera, TinyCad (v.2.8).MVSwasfabricatedandvarious The controlcircuitwasdesignedusingthesoftware &M anagement demand andrequirement. Milkisabasicraw desirable productswhich meetthecustomer earning frommilkbypresenting innovativeand Value additionisamechanism toaccelerate the in Co-operativeSectorHaryana Value Chain Analysis ofMilk and Milk Products long termperspective. across gender is socially unsustainable from the less arduoustasks.Suchkindofdivisionlabour time (26% of work hours) in dairy and perform the dairy farming activities. Men spend much lesser in hours work their of 44% about spend and etc. cleaning, field, from fodder and grass bringing of season. The femalescarryoutthedrudgerous task Timila and Bhimal, especially inthesummer conventional feedresources like, leavesofBanjh, 25/l, respectively.Thefarmersareusingnon- price of cow and buffalo milk was households wereits members. The producer sample the of 35% only as smallholders the societies in theregionhavepooroutreachamong channels. Thedairycooperative milk marketing unorganised the mainlythrough marketed, is output milk the of 43% only production, In Uttrakhandregion,duetolowlevelofmilk unsustainable. existing productionsystemwouldberendered in thefastchangingmarketenvironment, that suggest practices farming dairy unscientific resources usedforgrazinganimalsand property very poor.Thedeplorableconditionofcommon practices followedbythelivestockkeepersare Chattisgarh. Thebreedingandmanagement predominantly ongrazinginthestudyareaof day, respectively).Theindigenousanimalssubsist in thetribalregionofChattisgarh(0.9and3.9l/ respectively) as compared to their counterparts in thehillregionofUttrakhand(2.2and6.0l/day, is higheramongthesmallholderdairyproducers cattle The averagemilkyieldoflocalandcrossbred Environments Production inUnder-developed System Production Sustainability ofSmallholderDairy on peranimalbasis. estimating the fixed and variable cost components process. Thesoftwarehasbuild-infeatureof used aswelloutputgeneratedintheproduction inputs variable and fixed of prices and quantity to thebreeding,feeding,managementofanimals, households and thedetailinformationpertaining enumeration ofthemilchanimalstock to villagelevelinformation, complete pertaining ` 22/l and ` of 0.11–0.97indriedacidcasein preparedfrom i.e., 25°,35° and 45 °C over a wateractivityrange desorption) characteristics at three temperatures, approaches, topredictsorption (adsorptionand based upontheaforementioned softcomputing Also, the intelligent models were developed between 5.54and9.54fordesorption. accuracy between5.48and10.60foradsorption sorption model;thatis,theGABmodelwithan for desorptionandthatofthebestclassicalempirical and 4.93foradsorptionbetween2.494.50 the connectionistmodelwithaccuracybetween2.52 Square percent error (RMS%) as compared to that of 0.23 and0.35fordesorptionintermsofRootMean between 0.09and0.34foradsorption to exhibitthebestwithpredictionaccuracyranging performance oftheneuro-fuzzyhybridmodelseems models) thatwerealsodevelopedinthisstudy.The models (i.e.,BET,Caurie,Halsey,Oswin,andGAB was comparedwiththatoftheconventionalempirical prediction potentialofthesesoftcomputingmodels The Nutrimix. fortified food, weaning millet–based water activityrangeof0.11–0.97inamilkandpearl temperatures, that is, 15, 25, 35, and 45°C over a (adsorption anddesorption)atfourdifferent been developed to predict the sorption isotherms Fuzzy Inference System (ANFIS) models have approaches: connectionist and Adaptive Neuro- Several intelligentmodelsbasedonsoftcomputing Techniques Selected Dairy Products Using SoftComputing Modelling theMoistureSorptionIsothermsin double tonnedmilkis`32perlitre,respectively. is `40,tonnedmilk36and ` 44,standardmilk full creammilkis is`329perkg, butter 377 perkg, state. Presently,theconsumerpriceofgheeis` ofthe locatedinvariousparts booths/outlets milk These milkandproductsaresoldatabout89 Paneer, Lassi, Chhach,Ice-cream,Ghee, butter, etc. dairy products,viz.,Paneer,KajuPinni,Milkcake, the plants also manufacture various value added respectively. Alongwithpasteurisedliquidmilk, andSirsa, Ballabgarh Rohtak, in Jind,Ambala, per day. The Co-operativeplants arelocated with amilkprocessing capacity of4.7lakhlitres Co-operative sector are 5dairyplantsinthe there and marketingofmilkinthestate.Presently, addition value procurement, in role significant playsa sector dairy Organised country. in the commodity milk isthesinglelargestagricultural to milkproducers.Intermsofvalueoutput, This also pricehelps to provide remunerative value addition. of milkisincreasedthrough unit material foralldairyproductsandearningper –– DairyEconomics, Statistics&Management ––

59 ndri annual report 2013-14 60 ndri annual report 2013-14 high earning ` commercial dairy farmswasfoundconsiderably dairy farms,respectively. Theprofitabilityof small, medium and large categories of commercial was foundtobe`226.85,220.15and ` 197.90on crores. The costof maintenanceofamilch animal farms weremakingtotalinvestmentupto`1.5 dairy commercial the of 58% Around increased. investment perSAUdecreasedastheherdsize per farm increased with herd size, whereas the 5.57 crores on large farms. The absolute investment farm rangedfrom` was intensivewiththeaverageinvestmentper the capitalinvestmentoncommercialdairyfarms ratio analyses.Itwasobservedintheanalysisthat Pradesh. The dataweresubjectedtotabularand dairy farms drawn from Jabalpur district of Madhya for thestudywerecollectedfrom60commercial enterprise to tap the market opportunities. The data dairy farminghastobetakenupasacommercial milk andproductsisincreasingrapidly,the dairy and milk production. As thedemand for witnessed considerableconcentrationtowards an appreciableextent.Indianlivestocksector and acquiredcommercialcharacteristicsto recent yearshasundergonemassivechanges The transformation in Indian dairy sector in the in JabalpurDistrictofMadhyaPradesh DairyFarms Economic ViabilityofCommercial acid casein. content in the fortified Nutrimix powder and dried models to effectively predict equilibrium moisture models and theconventionalempiricalsorption alternative technique over simple connectionist ANFIS model can potentially be used as a superior It was inferred that the hybrid soft computing RMS% between1.56and4.08 characteristics, the connectionist models attained desorption for while RMS%, 2.60 and 1.32 characteristics with anaccuracy ranging between connectionist modelspredictedtheadsorption The 4.95. and 1.4 between ranging RMS% with model empiricalGAB conventional best of the RMS% between 0.15 and 0.33 as compared to that desorption characteristics,ANFISmodelsattained for while 5.78; and 1.93 between RMS% attained à-vis thebestclassicalempiricalGABmodel,which accuracy ranging between 0.09 and 0.2 RMS% vis- predicted theadsorptioncharacteristicswithan models ANFIS The data. sorption the fitting for used were GAB) and Mizrahi modified Caurie, sorption models (i.e.,Oswin,Smith,Halseyand buffalo skimmilk.Severalconventionalempirical –– DairyEconomics, Statistics&Management –– 6.82. lakhs, ` 70.77 lakhsonsmall farms to 20.02 lakhs and ` 41.40 moderate togoodlevelofperformance. study, only a handful of 15 DCS had registered procurement. Outof344DCS analysedinthe due to poor membership and low level of milk societies (DCS)was farfrom satisfactory, primarily performance of most of the dairy cooperative indicated thatthephysical and thefinancial milk producing district in theregion.The results from samplehouseholdsinPulwama,thetop Cooperative Limited and primary datacollected secondary data collected from J&K Milk Producers onin Kashmirregion.Thestudywasbased performance and impact of dairy cooperatives The study wascarried outtoevaluatethe in KashmirRegion Co-operatives Dairy and Impactof Performance ranged between7to11%. total costsonfarmsandthereplacementcost the of 50% than more represented Concentrates productivity. tomaintain concentrates high farms werereplacementofanimalsandfeeding important packageofpracticesobservedonthe the increaseofherdsizeormilkproduction.The costs per animal or litreofmilk decreased with farms experiencedeconomiesofscaleasthe Thecommercialdairy productivity. milk higher farms could befurtherimproved byattaining financial performance.Theprofitabilityofthe returns to owner/investor and havefavourable promise higher farms werecapitalintensivebut commercial dairy the though It wasinferredthat on small,mediumandlargefarms,respectively. herd sizewasfound48,173and430milch animals animal and herd size, respectively. The breakeven than thebreak-evenpointmilkproductionper average herdsizekeptbyallfarms, were higher realised and farms. Theactualmilkproductivity small farms as compared tomedium and large nature. Alltheratiosweremorefavourableon in ayearandalsoanindicationofcapitalintensive once only investment ofcapital recovery revealing on commercialdairyfarmswaslittlemorethanone better thanstandardvalues.Thecapitalturnover times three were ratios efficiency and solvency dairy farms was favourable as theliquidity, average. The financial performance of commercial farms was estimated to be` 32.78 per litreon an The cost of milk production on commercial dairy day werereducingwiththeincrease of herd size. of herdsize,thenetreturnspermilchanimal increase returns perdaywereincreasingwith net medium andlargefarms,respectively.Whilethe lakhs ofnetreturnsperfarmannumonsmall, respectively, withanoverallaverageof` for small,mediumandlargeherdsizecategories was workedouttobe`23.12,22.93and24.30 Cost perlitreofmilkformilchcrossbredcow 1.43. an overallaverageof` of householdswith be of milkwasfoundto per litre return Net per litre. respectively, withanoverallaverageof` small, mediumandlargeherdsizecategories, worked outtobe`27.07,27.16and28.77for Cost perlitreofmilkformilchbuffalowas Chain inKarnalDistrictofHaryana Economic AnalysisofTraditionalMilkMarketing support servicestothecooperativemembers. of milch provisioning of animals inputthrough and aboveall,targetingonraisingtheproductivity milk coolers to facilitate milk collection twice a day, aggressive membership expansion, installing bulk procurement, severalstepsarerequiredsuchas procurement increases.Inordertoenhancethe proposition fortheDCSunlesslevelofmilk asustainable be priceswouldnot procurement From thelong-run perspective, payment of higher higher procurementpricespaidbytheco-operatives. participating incooperativemarketchanneldueto from milkproductionwashigheronhouseholds were providedtoitsmembers. Thenetincome region as no input or extension support services impact ontheproductivityofmilchanimalsin The cooperativesdidnothaveanysignificant alternate milkmarketingchannels. socially moreactive,preferredcooperativesto centres. Theyoungerfarmersandthosewhoare rather thanreaching out to themilk collection in theregionpreferdoor-stepcollectionofmilk location ofthemilk collection centres. The farmers in cooperatives were procurement prices and The importantdeterminantsofmarketparticipation marketing chainconsisting ofmilkvendors, marketed surplus disposed off through traditional 92% about that showed milk of pattern disposal to informalsectordispose offtheirmilk.The The householdinthestudy areahadpreference increased withincrease inherd size category. marketed surplustothehouseholdproduction of households,respectively.Thepercentage for small,medium and largeherd size category of the households was 67.98, 70.02 and 71.21 percent Marketed surplusaspercentageofmilkproduction an overallaverageof`0.73. 0.92 and`0.45across respective categories,with litre. Netreturnperlitrewas found tobe`0.53, ` 1.32,1.50,1.20across respective categories 23.35 per 23.35per 27.50 IV: Producer-MilkVendor-Contractor-Consumer Channel- Vendor-Consumer; Consumer; Channel-III:Producer-Milk Channel-I: Producer-Consumer;Channel-II:Producer-Creameries- litre), respectively. Creameries (`5.16/litre)andContractors3.56/ Vendor-A (` the intermediaries involved in the chain viz., and for crossbred cow milk ` observed lower, i.e.,forbuffalomilk`1.43/litre producer’smargins wereand producers,the intermediaries different receivedby of margins than Channel-IIIandIV.Asanoverallcomparison method showed that Channel-II was more efficient efficiency worked outby using Acharya’s modified spread waslowestincaseofChannel-II.Marketing in consumer’s rupee was highest aswellprice in theprocessofmilkmarketing.Producer’sshare comparison where atleast one agency was involved in toconsiderationfor The channelsweretaken comprising ofmilkcooperativesocieties. chain marketing milk modern by 8% about only creameries/private dairies and contractors, while household and dietary pattern has the significant the has pattern dietary and household as wellfunctionaldairyfoods. Theincome of conventional preferred purchaselocation for both on monthlybasis.Supermarket wasthehighest while functionaldairyfoods were liketoconsume consume conventionaldairy foodsondailybasis to like would consumers of 51% About month. in eachhouseholdwasfoundtobe0.32kg/ about 0.36kg/month.Lowfatdahiconsumption 0.77 kg/month while fortified dahi was consumed average, eachhousehold consumes Yakult about consumption expenditureonprobioticlassi.Onan expenditure was on probiotic drinks while lowest year. Inallincomegroups,highestconsumption consuming functionalfoodsfromlessthanone were foods dairy functional of consumers 76% about thefunctionaldairyfoods.Morethan know to interested were consumers of 68.42% knew the concept of functional dairy foods. About respondents of 83% total, of Out respectively. parlour and20fromretaildairyproductsstores, selected as 70 from supermarket, 30 from Amul from thosepurchaselocationsrespondentswere functional dairyfoodswereselectedrandomlyand Mumbai andPune.Thepurchaselocationsfor selected for analysis of consumption patternfrom foods wasveryscanty.Total120respondentswere State asinformationavailableforfunctionaldairy The presentstudywasconductedinMaharashtra Foods inMetropolitanMaharashtra Dairy Functional Fermented for Preferences A Study on Consumption Pattern and Consumer 7.45/litre),Vendor-B (` –– DairyEconomics, Statistics&Management –– 0.73/litre than 3.26/litre),

61 ndri annual report 2013-14 62 ndri annual report 2013-14 (0.47), fruitsandvegetables (0.79),spices(0.65), (0.37), edible oils and fats (0.29), fish meat and egg pulses and their products (0.48), sugar and jaggery liquid milk (0.46),cereal and theirsubstitute (0.27), expenditure elastic. The expenditure elasticityof relatively were items these that reflecting (1.22) and refreshment(1.29),totalnon-fooditems khoa, sweetsandotherproducts(1.09),restaurant (1.75), paneer(1.55), (1.72), desighee(1.12),butter more thanunityincaseofmilkpowder(1.84),curd study, expenditureelasticitywerefoundtobe households thanvegetarianhouseholds.Inthis found to be of highermagnitude in nonvegetarian percentage of total expenditure on fluid milk was and was foundtobe`700.27innonvegetarianclass monthly expenditureonmilkandproducts increase in family budget. Further thepercapita pattern ofmilkandproductsincreased with Consumption (0.25%). butter and (0.54%) curd powder (0.93%), (1.60%), khoa (1.00%),milk ghee desi (1.61%) paneer (3.17%), sweets by followed expenditure on liquid milk was the highest (4.28%) Among the individual milk and milk products, income. of level in increase the with 17.34%) to 10.14% (from increased products milk and milk on share ofexpenditure percentage observed that and semi-skilled labour class(` (` 530.23),businessandretailersclass408.18) (` higher quantityof milk and milk products groups revealedthatprofessionalclassconsume and milkproductsamongdifferentoccupational of milk non fooditems.Theconsumptionpattern 55.97 percentoftotalexpenditurewasspenton food items includingmilkandproducts on spent was 44.03% expenditure total this of out different occupationgroupswas` The average percapita monthlyexpenditurein to be 67.50%, 41.67%, 41.67%, 26.67%, respectively. whitener andbabyfood,khoacurdwerefound Households consuming butter,milk powder as atea paneer andsweetsotherproducts,respectively. 77.50% of sample households consume desi ghee, and 80.83% 90.83%, households sample all by The study revealed thatliquid milkwasconsumed in LucknowCity(UttarPradesh) Consumption Pattern ofMilk and Milk Products categories. most preferred brand among thelow fatdahi the probioticdahi.Danonelowfatdahiwas b-active probioticdahi was most preferred among was the most preferred brand while Mother Dairy dairy foods.AmongtheprobioticdrinksYakult effect onconsumptionexpenditureoffunctional –– DairyEconomics, Statistics&Management –– 1106.07) followedbygovernmentservice class ` 600.49invegetarianclass. Surprisingly, 277.95). It was also 277.95). It wasalso 4447.37and

major marketing functionaries and brokers were major marketing functionariesand brokerswere Sellers,buyersandtradersweretheRajasthan. by DepartmentofAnimal Husbandry, Govt.of framework. CattlefairsofRajasthan werecontrolled statistical analysisformedthe basisofanalytical randomly forprimarydatacollection. Tabularand and 30buyersfromeachcattlefairwereselected during 2000-01to2009-10.Asampleof30sellers basis ofmaximumaveragebovinetransaction Tejaji, JaswantandPuskarwereselectedonthe of 10cattlefairs,4viz.,Ramdev,Veer faced bydifferentmarketingfunctionaries.Out animals andmostimportantly,theconstraints pricesofdifferent affecting each channel,factors in efficiency marketing and costs associated with transaction ofdifferentlivestockspeciesalong functional structures,channelsoperatingfor detailed study ofexisting organisational and The presentstudy wasundertakenthrougha Organised CattleFairsofRajasthan Economic AnalysisofBovineMarketingin milk andproducts. significantly increased theper capitaexpenditure capita expenditureonfruitsandvegetables(FVEG) income of thehousehold (MINC) aswellper group showedthatanincreaseinthemonthly household (MINC).Theresultsfornonvegetarian food items (NFOOD)andmonthlyincome of the vegetables (FVEG)percapitaexpenditureonnon affected by per capita expenditureon fruits and on milkandproductswassignificantly vegetarian grouphouseholdpercapitaexpenditure expenditure onmilkandproducts.Incase of capita per the affect significantly and positively per capitamonthlyexpenditureonsugar(SUG) expenditure onfruitsandvegetables(FVEG), of thehousehold(MINC),percapitamonthly procedure and it was found that monthlyincome regression stepwise using by fitted was function look intotheeffectofthesefactors,consumption well associo-economicstatusofthehouseholds.To by expenditureonotheritemsofconsumptionas influenced greatly are products milk and milk of powder, curd,butter,paneer.Theconsumption lead togreaterchangesintheconsumptionofmilk anincreaseinincomewouldThis impliedthat that theseitemswererelativelyincomeelastic. curd (1.54), butter (1.72) and paneer (1.25) reflecting be more than unityin case of milk powder (1.49), total expenditure.Income elasticity werefoundto rise in decreases with expenditure proportionate i.e., essentialcommoditiesandindicatingthe these items were necessary for sample households, was observedtobelessthanunitysignifyingthat total foodincludingmilkand milk products (0.79) both inplainandhillyregions inthestudyarea. secondary enterprise for majority of respondents Agriculture was the primary and dairying was the Regions ofUttarakhand Farmers: A ComparativeStudy in Plain andHilly Communication Technology(ICT)byDairy Utilization PatternofInformation (96.67%) gotthehighestrankinKalyani. (84.44%) in Bangalore and ‘variety of fodder crops’ (80.00%) in Karnal, ‘conservation of green fodder’ care domain. ‘Round the yearfodder production’ (72.22%) in Kalyani got the highest rank in health ‘knowledge and diagnosis of common diseases’ and Bangalore in (90.00%) diseases’ common of diagnosis and ‘knowledge Karnal, in (77.78%) the highestrank.Similarly,‘vaccinationschedule’ (78.89%) in Bangalore and Kalyani, respectively got animal’ (86.67%) and ‘high yielding breeds of cattle’ animals to their region’ (80.00%), ‘selection of milch while farmersinKarnalranked‘SuitableCBdairy Dealers. Informationneedanalysisshowed Input Presidents, Stockmen,VeterinaryOfficersand were NDRI,Vendors,DairyFarmers,Panchayat system identified at Karnal, Kalyani and Bangalore The major actors of local milk production innovation NDRI Perspective Study of MilkProductionInnovationSystem:A where significant factors that influenced prices was highestinit.Age,generalappearance,breed categories asproducer’sshareinconsumer’srupee efficient channel was Farmer-Farmer for all bovine for marketing of different kinds of bovine. The most and vi)Farmer-LocalTrader-DistantTraderexisted Distant Trader-Farmer,v)Farmer-DistantTrader Trader-Farmer, iii) Farmer-Local Trader, iv) Farmer- channels, viz., i) Farmer-Farmer, ii) Farmer-Local distributed thanbovinebuyers.Sixmarketing bovine sellerswererelativelymoreinequitably Further, respectively. (10.88%), buffalo milch and (12.17%) bull buffalo (12.58%), stock young cattle (17.68%), cow milch by followed (30.80%) share sellers andbuyers,bullocksaccountedformajor buyers. Outoftotalbovinetransacted by sample states whichdiscourageditspurchasebyoutside on male cattle below 3 years age from going to other 4.93 and4.04percent,respectively.Therewasban expenditure duringsameperiodgrewataCAGRof over 1996-97to2010-11.However,incomeand respectively -1.94%, and -1.62 -1.51, of (CAGR) declined withaCompoundAnnualGrowthRate banned. Arrivalofcattle,buffaloandtotalanimals D airy E xtension for VSs for field visit, arbitrary target fixation for for fixation target arbitrary visit, field for VSs for facilities oftransport Lack relationship. negative posting, span of control and job stress had significant with jobperformanceandsatisfaction. Distanceof involvement had positive and significant relationship received, sourceofinformationutilization,andjob job satisfactionlevel.Age,serviceexperience,training level ofjobperformanceandmoderateresponseto of control. Majority of respondents had medium short serviceexperience(<8years)andmediumspan belonged toyoungagegroup(upto35years),had Majority ofVeterinarySurgeons (VS)weremale, Veterinary SurgeonsinHaryana Job PerformanceandSatisfactionof in bothregions. were the major constraints faced by thefarmers of ICTs and lackoftimeinteresttouseICTs cost and longhourspowercut,high or frequent failure lack ofpower,electricity of infrastructure, was foundtouseKCC.Inadequateavailability while inhillyregionsnoneoftherespondent mobile phoneforcallingKCC(KisanCallCentre) used plain region,morethanone-thirdrespondents ICT, owned by almost 98 percentrespondents. In Uttarakhand. Mobilewas most the widely used Opportunity Index (DOI), was low (0.12) in Availability of ICTs calculated using Digital fairs ofRajasthan. are suggestedtoimprovetheperformanceofcattle supply of technological inputs in adequate quantity states, improvingmarketinformationservicesand male cattle below 3 years age for taking to other imposedon ofban withdrawal transaction, after of infrastructure,permissiontobuyersleave Rawanna dateweremajorconstraints.Development as wellfeedsandfodderatfairsdelayin buffalo bulls.Forsellers,inadequateinfrastructure main reasonsforpurchasingcattlebullocksand in agricultural operations and transportations were reason forpurchasingmilchanimalswhereas,use of bovine.Rearingformilkpurposewasthemain were themajorreasonsforsaleofdifferenttypes cash requirementandscarcityoffeedsfodder Immediate prices. influenced that factors major mammary systemandbreedfordrybuffaloeswere for lactatingbuffaloesandgeneralappearance, Further, breed,milkyieldandgeneralappearance and breed were the major factors thateffected price. dry cows,generalappearance,mammarysystem breed and general appearance whereas in case of of bullocks.Incaselactatingcows,milkyield,

63 ndri annual report 2013-14 64 ndri annual report 2013-14 overall annualincomeof farmers. Theresult Rearing Pradesh Sustainability ofGangatiri study area. the majorconstraintsfacedbyfarmersin were inbuffaloes todetectheatsigns knowledge supplement feed/mineralmixtureandinadequate timely vaccinationfacilities,pooraffordabilityof practices. Highcostoftreatment,unavailability correlated withtheadoptionofbuffalohusbandry were foundhighlysignificantandpositively consumption, milksaleandoverallknowledge cosmopolite channel,landholding,herdsize,milk mass mediaexposure,personallocalite, in thestudyarea.Thefurtherexploredthat and calving interval, 450.2±35.1 days/buffalo found service perconception,2.57±0.45times/buffalo; days/buffalo; service period, 142±26 days/buffalo; 156.4±39.6 days/buffalo; age at first calving, 1482±162 buffalo; peakyield,8.1±1.9ltire/buffalo;dryperiod, buffalo, lactationmilkyield,1636.4±189litres/ day/buffalo; lactation length,293.5±27.1 days/ explored thataveragemilkyieldwas,5.36±1.2litre/ of respondents (50.00%) in the study area. The study Crop farmingwastheprimaryenterpriseformajority Farmers inUnnaoDistrictofUttarPradesh Buffalo Husbandry Practices among Dairy trainee aboutimproveddairyfarmingpractices. had increasedoverallknowledgeof that training wasconcluded It intraining. trainers faced by privileged sectionofsocietyweremajorconstraints in time,largeoperationalarea,dominationof KVKs in the studyarea. Non-availability of funds farmers in effective use of trainings conducted by problem weretheseriousconstraintsfacedbydairy for greenfoddercultivationand repeat breeding low priceofmilk,inadequateavailabilityland non-trainees. Highcostofcommercialfeedand to compared as (40%) care health and (43.33%) practices management (48.33%), feeding and Trainees have slightly more knowledge in breeding Madhya Pradesh of inSatnaDistrict Farming Dairy on Kendra Impact ofTrainingsorganizedbyKrishi Vigyan performing theirduties. constraints experienced by veterinary surgeons in misadvise offarmersbyquackswerethemajor poor research-extensionlinkageintheDAH&D, consideration ofideasandsuggestionstheVSs, non- DAH&D, the by Insemination) (Artificial Al –– DairyExtension –– Gangatiri cattle contributed 56.73% to 56.73% contributed cattle Breed in Eastern Uttar Breed inEasternUttar was found significantly higher for beneficiaries than health carepractices.Theoverall extentofadoption who adoptedmoreontheaspects ofbreedingand non-beneficiaries to compared as beneficiaries the were foundtohavegreaterextent ofadoptionamong that feeding, management and fodder production minimizing theirtimeandcost.Theresultsdepicted towardsKCCformedium levelofgratification their expressed beneficiaries the of 63.00% Around information onimproved dairy farming practices. the respondentscontactedKCClevel–IItoobtain of 64.67% About non-beneficiaries. to compared annual income and information utilizationas status, operational land holding, milk production, Kisan Call Centre beneficiaries had better educational Adoption amongDairyFarmersofTamilNadu Impact ofKisanCallCentreonTechnological perceived bytheDhangars. different diseases werethemajor constraints Scarcity offodderandwaterincidences veterinary hospital or call veterinary personnel. large ruminantstheytooktheiranimalstonearest available ethno-veterinarypractices whereas for small animals at theirownhome by usinglocally their treated dhangars the of (65%) Majority source offeedandfodderfortheirlivestock. respondents reportedthatgrazingwasthemain the of (80.80%) the of Majority Pandhapuri). and Kandhari) andtwobreed ofbuffalo(Nagpuri and Sangamneri), onebreedofcattle (Red and Madgyal),twobreedsofgoat(Osmanabadi reared mainlytwobreedsofsheep(Deccani Dhangars respondents. the of (62.50%) majority Livestock rearingwasthemainoccupationof Nomadic TribeofMaharashtra Livestock-rearing PracticesamongDhangar maintained easily with locallyavailable resources. rear underlocalclimaticconditionandcanbe Farmers perceivedthatGangatiri holding, milk production and extension contact. education status,herdoperational positively and significantly associated with Sustainability ofGangatiri cattle rearinghadlineagetowardsustainability. CSI score ranging from0.3to0.6.Suggestingthat 1, and most of respondents (67.59%) had medium index (CSI),whichrangedfrom0to sustainability rearing ofrespondentswasmeasuredascomposite only duringgovernmentprogramme. The cattle diseases. Majorityofrespondentsdidvaccination bloat andmastitisweremostfrequentlyoccurring practised grazingofcattlefor6-10hrs.FMD, (53.70%) respondents of most that showed cattle rearingwas is suitableto were inbothlowerandhigherlevelcategory. whereas 35.13% of livestock rearers of alpine region region wereinhigherlevelofadoptioncategory coastal of rearers livestock of 37.75% that found region werehavingpositivescore.Itwasalso alpine of households the 99.17% whereas value household of coastal region werehavingnegative eastern coastaldistricts.Vulnerabilityvalueofeach Andhra Pradeshwastheleastvulnerableamong vulnerable whereasEastGodavaridistrictof Pudukkottai districtofTamilNaduwasmost were aware of changing climatic scenario. Further, livestock rearers of coastal and alpine, respectively, of 81.67% and 91.87 that revealed study The Coastal andAlpineRegionsofIndia Climate ChangeAmongLivestock-rearersin Vulnerability andAdaptationStrategieson of dairying. was difficulty in providing answers on specific areas major constraint of 96.67% of the level - I KCC officials 1551 from mobile and one rupee coin booth. The inaccessibility ofKCC’stollfreenumber1800-180- was respondents beneficiary the of 90.89% by agro advisoryservices.Majorconstraintexpressed dairy farmers had favorable attitude towards KCC beneficiary the of 47.00% About non-beneficiaries. yield and inter-calving period among the elite cows yield and inter-calving period among theelite cows average lactationmilkyield,dailypeak The birthweightofcalves was 7.91±0.58kg.The and urineclearedonceinonetotwomonths. spread everyday as bedding material over thedung coastal districts of Karnataka. Greenleaves are distributed predominantly in Malnad and adjacent The Malnad Gidda are unique dwarf cattle Cattle ofMalnadRegionKarnataka Evaluation and Improvement of Indigenous Nagi -wondercowgave20calvesin 25years S outhern R egional S ± 208.93 TLC/ul and 10.36 ± 0.32 g/dl, respectively. ± 208.93TLC/ul and 10.36± 0.32g/dl, respectively. hemoglobin content inMalnad Gidda was 8729.17 as sacred.TheTotalLymphocyteCountandthe animal areknownasKapile/Kapila in 25years. Malnad Gidda cattle withpale brown wonder cow has already given birth to20 calves of 396 uniqueness of regular calving (inter calving period reproductive months, respectively.Theyhavethe 2.17 ±0.29liters,3.420.39litersand14.910.93 liters, 69.40 ± 522.33 was condition field under health foods. was foundthemajorconstraintfornotbuyingsuch of significance. Among the constraints, availability consumers aswelldoctorsatonepercentlevel withtheperceptionof correlated significantly and familyhealthstatuswerepositively foods health foods, nutritionalinformationabout foods, informationandknowledgeabouthealth consumption ofhealthfoods,purchasing income, dietary habit, mass media exposure, study revealedthattraitslikeage,education, taste eitherextremelygood or slightlygood. The yogurt perceived 67.78% good. slightly or good extremely either milk flavoured about opined stakeholders of 63.33% foods. health about respectively, werehavinghighlevelofperception 50%, and 75 producers, and doctors of cases the perception regardinghealthfoods; however,in of level low had consumers the of (55%) Majority “availability” and“prices”ofhealthfoodproducts. of respondents”, “health foods followedby health of preference influencing factor important most “Nutrient contents” of the healthfoods was the based healthfoodproductsaspartofregulardiet. oftherespondentschoosedifferent dairy- Majority towards HealthFoods Preference and Perception of Stakeholders tation + 33 under days) low input regime. , B Elite MalnadGiddacowwithcalf angalore andconsidered – the Nagi – the

65 ndri annual report 2013-14 66 ndri annual report 2013-14 due toassociativeeffectof differentfeedstuffs. CS. NochangeintheIVFK ofCSwasobserved drawn from 6 homogenous groups to formulate made into homogenous groups. Feedstuffswere Heterogenous feedstuffs having similar IVFK were constant to derive fermentation kinetics (IVFK). subjected to first order kinetic model with one rate intervalswas time different at measured. IVGP dietswere based roughage (CS), 4complete supplements 12 3 roughages, feedstuffs, concentrate on 34concentrate In vitrogasproduction(IVGP) Concentrate Supplement Development ofProtocoltoFormulateDesigner exhaust fansandreflectiveroofcoating,etc. fans with foggers, fans with gunnybagcurtains, fans, fanswithsprinklers, viz., crossventilation, or the other micro-climate modification strategies of thesemen stations adoptedcombinationofone 4.58±0.38 kgand54.25±8.9gm,respectively.Most mixture fedwas29.55±2.23kg,3.44±0.29 green fodder, straw/ hay, concentrate, and mineral of quantity the average an On (4.76%). tiles and (9.52%) sheets aluminium by followed material bull sheds had asbestos sheets (85.71%) as a roofing m, 0.67±0.04 m, 0.29±0.01 m, respectively. Mostly mangerwas2.07±0.18 offeeding anddepth width, 5.35±0.32 m,respectively.Theaveragelength, roof height ateave and ridge was 3.49±0.17 m and 12.04±1.83 m in acoveredareaandopenwas ± SE)perbull loafing/open area. The average floor space (Mean tethered roundtheclockwithoutanyaccess to all thebulls,however,bullsinsevenshedswere was East-West. Individual pens were provided to The orientation of bull sheds in allsemen stations 627 breedingbullsin21shedswereevaluated. different partsoftheSouthernIndiamaintaining Nine commercialsemenstationslocatedin Evaluation ofCommercialSemenStation Friesian, Jersey,andDeonicattle. and IGFBP-3genesamongMalnadGidda,Holstein degree ofgeneticvariationinGHR,GnRHR,IGF1 and 0.30, respectively.Ourstudyrevealed a high males andGradedHFcattlewas0.014,0.00,0.143, A bovine beta-caseingeneshowedthefrequencyof and buffalo breeds of the region. Genotyping in cattle milkwasfoundtobehigherthanother as estimatedbySandwichELISAinMalnadGidda collection andpropagation. The lactoferrin content males basedonmother’smilkyieldforsemen programme toavoidinbreedingandselectedelite exchange The SRSofNDRIhasintroducedbull –– Southern Regional Station,Bangalore –– 1 allele in Malnad Gidda, Deoni, Holstein Friesian allele inMalnadGidda,Deoni,HolsteinFriesian 2 and16.45±1.55m 2 , respectively.The W Digestible CP,NDFandcelluloseintakes(g/kg feedstuffs were selected on the basis of IVFK. Synergistic effect ofCS(SCS) improved when SCM. In the present study, it was observed that the SCM. Inthepresent study, itwas observed thatthe of occurrence the influenced factors genetic non respectively. Season being one of the important (23.95%), parity third and (25.15%) second by followed (25.75%) parity fourth in highest was incidenceofSCM the that was observed farmers. It marginal or small by practices hygiene sufficient mastitis inordinaryhousingwascontrolledby which indicated that the occurrence of subclinical floor kutchha with combination in roof tile with shed in cows of 3.17% as lowest be to found was SCM 8.47%. as floor concrete with roof asbestos up ofstoneslabfollowedbyanimalsunder made flooring and roof the on tiles indigenous with sheds at managed animals in 14.19% as district of Karnataka,SCM was recorded highest Kolar at field farmers’ at maintained animals study conductedon1005HFgraded/crossbred housing, parity,season,etc.Underthepresent various genetic andnonfactors including farmers. The occurrence of SCM depends on threat causes high economic losses to the dairy Sub clinicalmastitis(SCM),amajorandsilent Mastitis Role ofNon-geneticFactorsAffectingSubclinical ● ● ● ● ● ● ● findings: due to SCS. Project was concluded with following was observed solids (TS)andsolids-non-fat(SNF) 1 to 5 % (P = 0.17). Apparent improvement in total MBP (P<0.01)andmilkproductionwasimproved

0.75 however, improvement in SNF % was observed. observed, was yield milk in trend definite No between grainandOSM+AICPonMPP. Characteristic difference in IVFK was observed %) comparedtopelletfeeds. 17 to 13 increase mean (overall % 15 about by improved (MPP) production protein Microbial Digestible nutrient intake of NDF was improved. unaltered intheCS. remained was feedstuffs different of IVFK feedstuffs weregroupedtohomogenoussets. heterogenous rate fermentation on Based oilseed meals. IVGP of grains = agro-industrial-co-products ≥ ) were improved because of 15 % increase in increase % 15 of because improved were ) maintained assuchinloose housingsystemand In group II (untreated control), 10 heifers were feeding alongwithnutritional supplementation. total of10heifersweretied individuallyforstall of estrum and subsequent fertility.In group I, a CO-Synch andCIDRprotocoloninduction with SRS to evaluatetheeffectof supplemental feeding old (28 to42months)maintainedatCattleYard, 20 prepubertal Deoni heifers of 2.4 to 3.6 years Deoni animals.Hence,astudywasconducted on in calving first at age reduce and puberty induce explorevariousstrategiestoThere isaneedto Induction ofEstrusandFertilityAugmentation interventions. better nutritional and reproductive management reproductive performance in Deoni cattle through scope toaugment is further AFC andCIthere respectively. Thereisaneedtoreducedays as 434±4.7,2.7,86±1.6,71±4.0,148±3.7and282±0.4, days) andgestationlength(indays) wereobserved service (FTSS/BIindays),period(SP successful to first or interval breeding days), in service/voluntary waitingperiod(CTFS/VWP first to calving number), in (SPC conception per interval orintercalvingperiod(indays),services in parous Deoni cows, themean values for calving and 280.91,respectivelyinDeoniheifers. Similarly, days) was 994.50±16.84, 1324.32±19.38,2.33,95.58 (in service (FTSS/BIindays)andgestationlength in number), breeding interval or first to successful calving (AFCindays),servicesperconception(SPC first at age days), in (AFS service first at age for of the analysis revealed that theoverall mean±SE carried onfortheperiodof2002to2012.Results records ofDeoni cattle ofNDRI-SRSherdwas reproductive performance based on the pedigree on Therefore,thepresentinvestigation sector. of theseanimalsarescantyunderorganizedfarm importance. Studies on reproductive performance improvement oftheDeonigermpalsm is ofmost holder dairyproducers, theconservationand indigenous cattle and their rearing by small Pradesh. Consideringtheimportantroleof cattle of Maharastra, Karnatakaand Andhra Deoni isamediumsized,dualpurposeindigenous Organized FarmManagement Reproductive Performance of Deoni Cattle under to winterandrainyseason. level with less availability of fodder incomparison causing mastitiswerepresentatabovethreshold organisms the probably (9.88%), seasons rainy and (38.37%) winter by followed (52.33%) season highest incidenceofSCMwasrecordedinsummer (Cloprostenol-Vetmate between theSSCP pattern ofATP1A1 gene with observed was association significant The calving. the observed SNP positively affected the age at first negative association with lactation length, whereas yield. The genotype AA atposition 15739 showed a association withlactation length andlactation AA genotypeatposition4649showedpositive 4291, TTgenotypeatposition4394and4472 Deoni cows having CT genotype at position using Rhoads formula and used in analysis. was calculatedfrom recorded rectal temperature Deoni cattle. The Heat Tolerance Coefficient (HTC) and lactationyieldcomparedtoothergenotypesin length lactation higher had 82.5% of frequency a loci C3076T in fragment3comprisingexon3, with Statistical analysisrevealedthatTTgenotypeat and five identified SNPs were in the coding region. which elevenSNPswereinthenon-codingregion of out cattle, Deoni of gene ATP1A1 in identified were custom sequenced. SixteenSNPs were were identifiedandcorresponding PCRproducts differences intheSSCPpatterns,allelicvariants GLM procedure of SAS software. Based on the performances was investigated and analyzed using thermo-tolerance, productiveandreproductive with polymorphism/s identified of association breed ofcattleusingPCR-SSCPanalysis.The entire codingregionwascarriedoutinDeoni The geneticcharacterizationofATP1A1genein tooxidativestress.response whichissensitive ATP1A1 gene isacandidateforheatshock in DeoniCattle Thermo-Tolerance and Production Performance Association ofATP1A1 Gene Polymorphism with rate of80%. found pregnantwithasynchronizedpregnancy and ultrasonography. Outof10heifers,8were CIDR (EAZIBREED CIDR injection i/[email protected]/heiferandwasinsertedwith using progesterone based assay kit (HeatAid inseminated heifers onday30postinsemination and no AI was done. Pregnancy was confirmed in signs showed heat of theheifersincontrolgroup injection i/[email protected]/heiferat72hours.None group wasadministeredGnRH(Receptol programme. On day 0, each heifer in treatment deworming drugaweekbeforesynchronization start ofsupplementalfeedinganddeticking/ were administereddewormingdrugpriorto HeifersingroupI, farmmanagement. routine they hadfreeaccesstofeedandwaterunder and 72 hours afterPGF 48 time AI(FTAI)wasperformed at day 8.Fixed i/m @ 2 ml/heifer followed by CIDR removal on ® ) injection was administered ) injectionwasadministered –– Southern Regional Station,Bangalore –– 2 α injection, with GnRH with injection, α ® 1380).Onday7PGF ® ® 2 α ) )

67 ndri annual report 2013-14 68 ndri annual report 2013-14 (exon 2.6)andC2600Tinexon2of TLR2 2.3), of sixSNPsviz., was carriedout.Itfound thatthepresence SSCP analysis whole exon2andthen covering the TLR2 gene was amplified of in overlapping 2 fragments exon while fragment whole a as amplified carried outbyPCR-SSCPanalysis.Exon1was Molecular characterizationofTLR2genewas cows. crossbred Friesian exon 2in214Holstein genetic polymorphismofTLR2geneexon1and The presentstudywascarriedouttoinvestigate Holstein FriesianCrossbredCattle Association with SomaticCellScoreinMilkof Polymorphisms (SNPS) in TLR2 Gene and its Identification of Single Nucleotide resulted insilentmutations. and Jerseycattle HF cattle andexon10inboth HF in exon 3 and 4 inJerseycattle,exons 7 and 15 in Isoleucine to Valine.The detected polymorphisms 7947 resultedinsubstitutionofanaminoacidfrom gene. Theobserved polymorphism (AG)atposition HF revealedAGtransitionatposition7947ofASS confirm polymorphisms. The analysis of Exon 2 in corresponding PCRproductsweresequencedto SSCP patterns, allelic variants wereselected and PCR-SSCP analysis. Based on the differences in the in all15exonsofASSgeneweredeterminedby were detectedinthestudy.Thegeneticvariants high salt method. No carriers of citrullinemia 78 Jersey bulls was extracted from blood using gene. GenomicDNAfrom131IndianHolsteinand to characterize Argininosuccinate synthetase(ASS) mutant allele of citrullinemia in the population and and Jerseymalestoscreenforthepresenceof SSCP analyseswerecarriedoutinHolsteinFriesian In thepresentinvestigation,PCR-RFLPandPCR- by digestingtheresultingamplicon with Ava ASS thymine transitionatcodon86fromexon5ofthe Synthetase. The disease is caused by acytosineto Argininosuccinate enzyme the of deficiency a of dysfunction anddeathwithinoneweekasaresult (ASS). Affectedcalvesdisplaysevereneurological urea cycleenzyme,ArgininosuccinateSynthetase the of deficiency to due metabolism urea of error Citrullinemia is(recessiveautosomal)aninborn Males Synthetase Gene in Holstein Friesian and Jersey Molecular CharacterizationofArgininosuccinate for developingmarkersthermo-tolerance. ATP1A1 gene could be used as a candidate gene physiological parameterswhichindicatedthat –– Southern Regional Station,Bangalore –– gene that can be amplified by PCR and verified C2155A (exon2.5),G2281C A827G (exon2.2),C1088G G2410A (exon 2.5),G2410A II. (R significant difference of Coefficient of determinants of FLMYi.e.516.11±40.30kg.Oncomparison no information was close to its true phenotypic mean genotype excluding LSM estimated The 79.59%. information wasestimatedat518.33±39.51kgwith LSM ofFLMYwithoutincorporatinggenotype 46.05 kg with 79.73% coefficient of variation (CV). information was549.98± incorporating genotype (PPARGC1A). Least squares means (LSM) of FLMY Activating Receptor Gamma Co-activator-1-alpha (CYP11B1) and Peroxysome Proliferator of Cytochrome P450-11-beta hydroxylase-1 technique andincorporatinggenotypeinformation milk yieldwascomputedbyleastsquares lactation first of value breeding Genetic meriti.e. breeding and modern molecular techniques. lactation milkyield(FLMY)conventionalanimal Bangalore topredictthegeneticmeritoffirst Station ofNationalDairyResearchInstitute, maintained atCattleYard,SouthernRegional The study was conducted in 108 Deoni cattle Genotype Information Milk YieldinDeoniCattleIncorporating Estimation ofGeneticMeritFirstLactation than thegenotypeTT(LSM3.375±0.176). showed moderately significant lower SCS (p< 0.10) found that thegenotypeCC (LSM 3.089 ± 0.197) including allthegeneticeffectstogether,itwas used was analysis squares least for model fixed susceptible. SimilarlyforSNPC2600T,whena resistant tomastitisandgenotypeAGwashighly GG were more contrary, animalswithgenotype thus, susceptible to mastitis infection. Onthe an increased number ofsomatic cells in milkand crossbred animals.ThisSNPwasassociatedwith to presenceofSNPG2410A,inthestudiedHF AA (LSMforSCS3.375±0.176)resulteddue of genotypeGG(LSMforSCS3.089±0.197)to cell score (SCS). In this investigation, a change somatic with 0.1) (p< associated significantly be gene., SNPsG2410AandC2600Twerefoundto excluding genotypeinformation. information was more precise than the model r and 0.680,respectively.Lower AICandhigher incorporating genotypeinformation were1001.70 0.728. Whereas,thosevalues for themodelwithout model incorporatinggenotypewere830.16and the of value phenotypic true the with coefficient Criterion (AIC)andSpearmancorrelation 0.975 and0.973,respectively. AkakikeInformation and 94.69% and 95.11 was which information incorporating and without incorporating genotype true phenotypevaluewasobservedinthemodels s 2 valueindicatedthatthemodel withgenotype 2 -value) and Pearson correlation coefficient with (RLH) hydrolysate was 8 and 18 or 19%, respectively. powder mixbase(PMX)and, protease(PTH)orRL 13% and hydrolysate was 8 %. Protein content in the hydrolysis. Drymatter(DM) content ofresiduewas optimum basedonproteinrecovery anddegreeof were 20% to 10 and 2.0% to 1.5 of concentrations RL and Protease respectively. 20%, and 3 2, were higher whenprotease,trypsin andRL concentrations was hydrolysis and yield Protein mix. fish-CW of Protease, trypsinandRLwereusedforhydrolysis FW wasdilutedin1:2ratiowithwater,bufferorCW. product andreducingenvironmentcontamination. (FW), cheesewhey(CW)andRL(RL)tonutritional waste fish conserve to undertaken was study The to GrowingCalves Fish By-productasAnabolicDietSupplements and Dairy from Hydrolysates Protein of Synthesis CV inMYandapparentlyimprovedmilkfat. were comparable. ACPs supplementation reduced efficiency and composition MY, but, grain than ACPs on lesser were efficiency and production between groups.StudyrevealedthatMBP comparable was production milk of Efficiency in CG, T1, respectively T2, T3 and T4 (P=0.56). (1 to 9%). Milk fat was 3.65, 4.05, 4.0, 3.7 and 4.17%, TGs than %) (16 CG in higher was (CV) variation of Coefficient 0.72). = (P kg/d 3.90 and 3.90 2.78, was3.47,3.85, corrected MYincorrespondingGps Fat 4% (180). T4 and (54) T3 (40), T2 (69), T1 than (225) CG on more was (gN/d) MBP significantly. differed intakes (P<0.05) fiber detergent acid and (EE; P<0.01) extract CP (P<0.01),ether digestible straw intakewas comparable. Total(P<0.01)and intake from CS were different (P<0.01) but, salt and made isonitrogen by 1 to 3% urea. Nutrient 2% mixture, mineral 3% with fortified were (CS) Supplements (T4). 50%GGC+50%BGH and (T3) by sorghumgrain(CG),WB(T1),SH(T2),HBC kinetics. TwentyDeonicows were supplemented was evaluatedusingin vitro gasproduction(IVGP) groundnut shellswas observed. Biological value pigeon peaandsoyhusks(SH);and,almond bean, hyacinth gram(BGH), (HBC); bengal chunis andhyacinthbean (GGC) green gram (P<0.01) ofwheat(WB)anddeoiled ricebrans; difference in proximate and cell wall composition milk yield (MY) and milk composition. Significant products (ACPs) onmicrobial protein(MBP), and carbohydrates(CHO)inagri-industrial-co- Study evaluatedadvantageofcrudeprotein(CP) Cows Microbial Proteinand Milk Production in Deoni from Agro-Industrial Co-products onRumen Associative Effect ofCarbohydrateand Protein CaCl well followedbytheadditionof1drop1M of eachculturewasaddedin follows: 150µl plates. The turbidity test was standardized as bottom flat well 96 a in cultures selected the with screened was filtrate the and membrane) (PVDF skim milk were filtered through 0.45 µ syringe filter cultures wereused.Paneerwhey,cheesewheyand nos) i.e. 9Antagonisticculturesand7Department For theturbidity test, overnight starter cultures (16 viz., Turbidity test and Double layer plaque assay. TS and the lactose in thewheywas hydrolysed by 20% about to concentrated was whey paneer The were obtainedfromtheNCDC, NDRI,Karnal. report, probioticstrains of lassi andbuttermilk. Duringtheperiod under preparationofprobiotic whey inthe utilizing ofThe projectistakenupwiththeobjective Utilization ofLactoseHydrolysedWhey min andstoredat4 were preprocessed by centrifugingat3000gfor20 whey: 16andskimmilk:18samples.Thesamples collected for screening: cheesewhey:42,paneer the presenceofbacteriophages.Numbersamples Experimental Dairyofthestationandtestedfor and skimmilksampleswerecollectedfromthe The samplesfromcheesewhey,paneerwhey Resistance and Evaluation of Starter Culltures for the Phage Bacteriophages of Identification and Isolation 2% ofcommercialprotease. yielded hydrolysatethatwascomparableto1.5 catalyst as RL of 20% to 10 with ratio 1:2 in CW and was statistically insignificant. It was inferredthat FW (DMD) than PTH powder (72%) however, difference digestibility DM higher 7% had powder RLH 0.01). and RLH syrup was 8, 9, 7 and 8%, respectively (P< fermentation rateofPTHandRLHpowder, base (10%) were significantly (P< 0.01) different. syrup The and (7%) PMX of rate Fermentation syrup. (100%) sugar with ml/ml 282 to compared ml/ml and RLHwithsugarbase(60:40)was136130 of PMX,PTHandRLH,respectively.TGPon In vitrogasproductionwas157,212and214ml/g 37 wells andtheplatewasincubatedovernightat and skimmilkwasthenadded totherespective was collectedandstoredat4 solution and dahicultures.Theclearsupernatant possible presenceofphagesformozzarellacheese cheese whey and paneer whey.This indicates the and dahiculturewith cheese whey culture with supernatant inthewellforMozzarella cheese o C. Theresultsshowedthattherewasaclear 2 after that 150 µl of cheese whey, paneer whey afterthat150µlofcheesewhey,paneerwhey o C. Twotestswereattempted –– Southern Regional Station,Bangalore –– L. acidophilus cultures 0 C forfurthertests.

69 ndri annual report 2013-14 70 ndri annual report 2013-14 for extensionofitsshelflife. edible coatingscanberecommended for paneer paneer remainedgoodfor 20days.Therefore, coated film edible The days. 8 after unacceptable samples packaged in LDPE pouches became was leastpreferredasacoating.Uncoated paneer concentrate protein whey samples, film-coated (7.7) instarch-coated paneer. Amongst the edible acceptability ofcontrolwas5.8whileithighest stored well andwas of similar quality. Theoverall hand, paneercoated withstarch and casein uncoated paneerduringstorage.Ontheother considerable moistureandweightlossesfrom samples duringstorage.However,therewere the fat,proteinandashcontentsofdifferent in differences significant no were There storage. during intervals defined at changes sensorial and analyzed for physico-chemical, microbiological taken at0,5,10and15daysintervalwere Paneer samplescoated with ediblecoatingswere sensory characteristics of paneerwasdetermined. physico-chemical, microbialand coatings onthe 12°C upto15days.Theeffectofthesebiopolymer in 65µmthickLDPE bags and were stored at 10- After coatinganddrying,the samples were packed concentrate andtapiocastarchcoatingsdried. Paneer was coated withcasein, wheyprotein Products of Edible Filmsand Coatings forSelected Dairy Characterization andEvaluation Development, dahi. lassi withtheabovecombinationofwheyand were optimum for production of most desirable lassi preparation. Use of 20% water and 13% sugar the for dahi of 20% upto replaced be could whey while freshconcentratedandlactosehydrolysed whey mixture were used for lassi preparation, fermented wheyandfermentationofmilk revealed that milk can be replaced upto 10% when Thesensoryevaluationoftheproducts mixture. the fermenting milk andthen hydrolysed wheytothe then mixingwithdahi,andaddingthelactose fermenting the lactose hydrolysed whey and hydrolysed wheywithoutfermentation todahi, i.e., mixingoffreshconcentratedandlactose was added to milk at threedifferentconditions, The concentrated and lactose hydrolysed whey 12%). to adjusted (TS whey hydrolysed lactose replacing themilkwithconcentratedand achieved.Lassiwasprepared by partially could be at 42 was treated with 1% lactase enzyme and incubated using lactaseenzyme.Whentheconcentratedwhey –– Southern Regional Station,Bangalore –– 0 C for 60 min., about 50% lactose hydrolysis lactose 50% about min., 60 for C CLHW inkhoareduceda and lactose23.46,24.53respectively.Theadditionof content of35.08,29.9,fat22.0,21.7,protein15.64,16.45 controlkhoaandexperimentalhadmoisture that showed analysis chemical The moisture. 35% with 30% moisture content was comparable to control of parameters andrheological Thesensory parameters. CLHW wascomparabletocontrolkhoaforallsensory 10% of incorporation with made khoa. Thekhoa increased, withincreasingthelevelofCLHWin and chewinessdecreasedwhilecohesiveness showed thatthehardness,springiness,gumminess CLHW andcontrolkhoa.Therheologicalstudies higher browningcomparedtothatinkhoa with 10% with incorporation of 20% and 30% CLHW showed softness andgranularityofkhoa.Theprepared hydrolysed whey (CLHW) increased sweetness, lactose hydrolysis. The use of concentrated and lactose 74% in resulted minutes 120 for 45°C for incubated and (20%TS) whey concentrated to enzyme lactase of and lactosehydrolysedwheyinthepreparation Investigation wasattemptedtoutilizeconcentrated Hydrolysed WheyinthePreparationofKhoa Utilisation ofConcentratedandLactose KOH/100 g fat and ripening index of days ofripening.Maximum FFAof7.65mmol increasing levelsofincorporation tocheesetill150 moderately increasedduring theripeningwith protein productioninresistant starchaddedcheese the rateofincreasesloweddown. FFAandsoluble during initial90daysofripeningandthereafter, of increase inFFA and soluble protein content cheese starch addedcheese duringripening.Inulin added cheese compared to control and resistant progressed butthe pHdrop was maximum ininulin initially inallcheeseandagainincreased asripening cheese andripenedfor60days. pHdropped 4% of curd were incorporated at salting at2and ofprebiotics combinations with different curd of 2% and 1 at culture probiotic as rhamnosus functionality ofcheddarcheese.Lactobacillus with inulinandresistantstarchtoincreasethe An attemptwasmadetoprepareCheddarcheese Enhanced Functionality Technology ofSynbioticCheddarCheesewith was foundtoincreaseduringstorageperiod. count ofkhoapackedinallthepackagedmaterials a shelflifeofthreedaysat37°C.Thetotalbacterial pouches, vegetableparchmentpaperandAl-foilhad in threedifferentpackagedmaterialsthatisLDPE The experimentaland control khoasamples packed made by incorporation of 10% CLHW with with CLHW 10% of incorporation by made khoa . The findings showed that addition of 1.0% 1.0% of addition that showed findings The khoa. showed significantly (p<0.05) higher (p<0.05) significantly showed w , pH and increased acidity. , pHandincreasedacidity. 59.34% in Cheddar were rate rate khoa

observed at 150 cheese added starch resistant 4% in observed were index ripening and fat KOH/100g mmol ripening andthereafterthemaximum FFA of9.12 processed cheese were tried for flavouring purpose 45% of maida in biscuit formulation. Cheddar and biscuits. Rolledoatswereincorporated at25,35and Oats wereincorporatedin cheeseflavoured Biscuits byIncorporatingOats andCheese Process OptimizationforthePreparationof 30°C similartothecontrolsamples. 5°C andonlyforonedayat stored for9daysat be prepared withtheincorporationofwheycould shelf lifestudiesindicatedthatthelassi beverages observed to behigher thancontrolsamples. The The syneresisofwheyincorporatedlassiwas consistency, viscosityandindexofvalues. by reflected as effect thinning in resulted whey control samples.Theadditionofconcentrated with comparison in significantly differed whey prepared withincorporationofcheese/paneer oflassi characteristics ash. Thephysio-chemical 0.55% and lactose 3.13% protein, 2.22% fat, 2.08% TS, 17.98% were samples control of composition The ash. 0.6% and lactose 3.83% protein, 1.86% fat, 1.7% TS, 17.99% contained whey paneer from product the while ash 0.59% and lactose 3.76% whey contained 18.03% TS, 1.68% fat, 2.0% protein, paneer industry.Theoptimizedlassi from cheese and cheese of benefits economic for contributing whey couldbeutilizedasextendersandthus paneer and Cheese sugar. 10% incorporating by affecting thesensoryparametersoflassiproduced significantly without level 20% to up blended be concentrated whey with 11.5% TS could effectively for lassiproduction.Thestudyrevealedthat the productionofdahiwhichisbasematerial standards of toned milk which is generally used in the match to TS 11.5% to restricted was whey cheese and paneerwhey.The concentration of for the production of lassi byutilizing concentrated The studyaimedatoptimizingprocess conditions Production ofLassi Incorporation ofConcentratedWheyinthe added cheese. retained inresistantstarchaddedcheesethaninulin in synbiotic Cheddar cheese. More dietary fibre was texture thanothers.Similarpatternwasobserved and body brittle crumbly, inferior had but flavour added. Inulin incorporated cheese scored maximum during ripening irrespective of types of prebiotic showed more degradation of α-casein than β-casein th day ripening period. Electrophoretic study dayripeningperiod.Electrophoreticstudy

in 4% inulin added cheese till 90 days of of days 90 till cheese added inulin 4%

of 71.63% of

intense sweetenersinvarious proportionstoget and sweeteners bulk combining prepared by respectively (w/v).Thesweetener syrupswere solutions, sugar of 10-15% and 50% 30-40%, to mannitol solutionswerefound equalinsweetness 20% and xylitol 50% sorbitol, 60% sweeteners, Incaseofbulk respectively. solutions, sugar 18-20% and 10% 10%, to sweetness in equivalent acesulfame-Kandsucralosewereaspartame, holding at60 sweetness ofallthesweetenersupto30min respectively. There was no effect on quality of 1500 ppm,800ppmand1000 found tobe sucraloseandacesulfame-Kwereaspartame, The maximum acceptable concentrations of 12.5-25 ppm, 61.5 ppm, 2.8% and 2%, respectively. sucralose mixture were found to be12.5-25 ppm, acesulfame-K, aspartame,levulosemixture, Minimum detectable concentrationsofsucralose, and qualityofsugarsubstituteswasstudied. Firstly, thesweetnessintensity sugar substitutes. khoa jalebiusingsugar-freesyrupspreparedby The studywasconductedtodevelopthesugarless Jalebi usingSugarSubstitutes Technological StudiesonManufactureof biscuit formulation. rolled oats and Cheddar or processed cheese in quality biscuits can be prepared by incorporating biscuits, respectively.Itwasconcluded that good for Cheddar cheese and processed cheese added 0.60% and 0.62 4.32%; and 3.93% were contents β-glucan and moisture The incorporation. cheese seen thathardnessofbiscuitswasdecreased by processed cheesebiscuits,respectively.Itwas and Cheddar for acid oleic 0.169% to 0.082 and Similarly, FFAcontentswere0.104to0.190 processed cheesecontainingbiscuits,respectively. from 3.52 to 3.94% and 4.41 to 5.32% Cheddar and showed changesinmoisturecontent whichvaried and cheese incorporated biscuits. Storage study foroatof 165°Cfor25-27minwasoptimized based onthesensoryanalysis.Bakingcondition optimized were cheese processed of 40% and cheese Cheddar of 30% formulation biscuit the in and changesinvisco-elastic characteristics. While incorporated dough showed the reduced hardness oat 25% the to cheese of Addition acceptability. including overall highest inmostoftheattributes scored biscuits incorporated oat 25% containing Sensory evaluationresults revealed thatthesample 30%. to 0 for N 21.73 to 25.05 from hardness The oatincorporateddoughshowedthereduced oat incorporationthedoughhardnessreduced. basis. It was observed from textural studythatby flour on 50% and 40 30, viz. each, levels three at o C. Itwasfoundthat800ppm of –– Southern Regional Station,Bangalore –– Khoa

71 ndri annual report 2013-14 72 ndri annual report 2013-14 had ashelflifeof8daysat4 concentrated paneerorcheesewhey and ash.Dahipreparedusingblendsofmilk and protein content and higher level of lactose fat lower have they However, solids. total 11.5% were preparedusingblendsofwheyhaving homogenized after heattreatment.Dahisamples samples the for those with significantly differ not samples homogenizedbeforeheattreatmentdid and at theend of the10 hour fermentationinthe increase inacidity andpHlevelsduring net the and 4.3.Withregardtothestagehomogenization, corresponding values forpHwere4.4(control), 4.3, LA, respectively for control, 2:1 and 3:1 blends; the 0.808% and 0.803, 0.806, was acidity the samples, In thecaseofconcentratedcheesewheyadded pH were4.46(control),4.32and4.41,respectively. LA, respectively. The corresponding values for 0.824% 0.838, 0.815, was 3:1 and 2:1 of ratio the in from blendsofmilkandconcentratedpaneerwhey range. The acidity (% LA) of control and dahi made concentrated whey,however,waswithinthe product producedfromtheblendsofmilkand The totalacidityandpHofcontroldahithe blends. waslowerinmilk-whey incubation during of fermentation,asmeasuredbyacidityandpH, such adifference.Trialsshowed that thedegree for concentrated paneerwheycouldbeattributed level ofcalciumandlowerproteininthe of milkandconcentratedcheesewhey.Higher sensory properties than that made utilizing blends textural and concentrated paneerwheyhadbetter was preparedutilizingtheblendsofmilkand and wheycompared to control product. Dahi the dahisamplespreparedwithblendsofmilk in lower was gel the of firmness the that showed analysis of dahi.Thetexture for thepreparation utilize concentrated paneer whey and cheese whey In thepresentstudy,processwasoptimizedto Preparation ofDahi Utilization ofConcentrated Whey in the as control. observed to have similar sensory characteristics levulose andsucralosemixtureformulationswas with prepared that whereas chewy, and firmer developed sugar-free syrups were found slightly a total solids level of 11.5% could be blended with that paneerwheyorcheese whey concentratedto obtained inthisinvestigation, itcanbeconcluded to thecontrol product. Based on the results of 15 to 25%. 25%. to 15 of sugar syrup byadding maltodextrine in the range viscosity of the syrups was adjusted to that of 60% sweetness similar to that of 60% sugar syrup. The –– Southern Regional Station,Bangalore –– Khoa jalebi waspreparedusingthe 0 C which is comparable C whichiscomparable dough and baked at 120, 135 and 150°C for 120 min. dough andbakedat120,135 150°Cfor120min. semolina (20:6:1)toahomogeneous andsmooth was preparedbykneading chhana,sugarand podo duringbakingwerepredicted. physico-thermal andtextural propertiesofchhana using responsesurfacemethodology, andthe Baking conditionsofchhanapodowereoptimized Methodology of itsBakingProcessUsingResponseSurface Properties ofChhanaPodoandOptimization Evaluation ofPhysico-ThermalandTextural liter offortifiedmilkwasestimatedat`64. fortified products. The total cost of production per control and properties werecomparablebetween the physico-chemical,texturalandmicrobiological up to8daysofstorageat4°Candthechangesin even acceptable were milk fortified and control and polydextrose.Dahisamples prepared using emulsifier of presence the to due probably lower prepared fromfortifiedmilkwas,however, by subjective and objective methods, of the dahi properties. The texturalquality, as determined normal physico-chemicalandmicrobiological to dahiwithacceptablesensorypropertiesand three fortificants. Fortified milk could be converted The storage had littleimpact onthelevels ofallthe flavour of milk probably due to Maillard browning. milk whilesterilizationhadaffectedthecolourand fortified the of characteristics sensory the affect did not and boiling pasteurization like treatments phytosterols and soluble fibre (polydextrose). Heat requirement ofomega-3fattyacids(ALA), would providealmost theentirerecommended (serving size = 240 ml) of the fortified milk per day and 1gofpolydextroseper100g.Twoservings about 289mgofALA,412phytosterols control/normal milk. The fortified milk provided to comparable were milk fortified of properties milk. Proximatecompositionandphysico-chemical sensory and physico-chemical properties of liquid levels, respectively withoutaffectingtheinherent polydextrose can be incorporated at 0.5, 0.5 and 1% observed thatflaxseedoil,phytosterolsand combinations ofthefortificants tried,itwas different ofmilk.Basedonthe sensory attributes into milk atlevels thatwouldnotaffect the of flaxseedoil,phytosterolandpolydextrose Work was carried outto optimise theincorporation Soluble DietaryFibre with Omega-3 Fatty Acids, Phytosterols and Fortified Milk of Evaluation and Development acceptable quality. of dahi making for (v/v) 25% to up milk toned Chhana podo

amplitude was recorded atthe upper end of valueforhysteresis Thepeak andenergy. unit hysteresis amplitude, its of terms in quantified isotherm. Thehysteresisofthe controlsamplewas gravimetric method at20,30and4050 dietetic samples weredetermined bystatic sorption data forsugar soaked (control) and known wateractivity(0.11–0.97)and moisture desiccators containing saturatedsaltsolutions of investigated. Sampleswereequilibratedin jamun on itsmoisturesorptionpropertieswere sweeteners andbulkingagentindietetic The effectofsugarreplacementwithartificial Properties ofDieteticGulabJamun Moisture SorptionandThermodynamic temperature from120to150°C. followed logistic model kinetics (R 0.223 W/m.K, 0.112 to 0.105 mm to 0.359 from respectively ranged heat specific and volumetric conductivity, thermaldiffusivity up to 40 min and then decreased. The thermal springiness, cohesivenessandresilienceincreased with baking time and temperature whereas increasedhardness, chewinessandgumminess time and temperature. Textural parameters like area ratioincreasedwithincreaseinbaking like meancellarea,densityandtototal and 150°C.Ingeneral,crumbgraincharacteristics 120, 135 at 95.35, 101.49and112.08,respectively valueof15.67to initial of theproductfrom intervals.. Bakingincreasedthebrowningindex and thermal properties were measured at specific crust colour, crumb graincharacteristics, textural content, specific volume, weight loss, oven spring, During baking, the core temperature, moisture in themultilayer region(a control sampledepictedhysteresis phenomena agreed wellwithexperimental data. Onlythe 0.8. The predicted values for equilibrium moisture (R kinetics reaction order zero- and first- followed MJ/m behaviour of both products adequately up toa products adequately behaviour ofboth GAB’s modelwasfoundtodescribethesorption models. HendersonandCaurie’sisotherm Oswin, The experimental datawas modeled using GAB, water exhibitingnotemperaturedependency. curve withdistinctdeliquescenceinthecondensed II iostherm The sorptiondatamanifestedintype 3.55x10 Fick’s second law of diffusion and it increased from design. Moisture diffusivitywas modeled using face-centered centralcomposite responsesurface podo wasoptimizedas135°Cfor104minusing forbakingofchhana time-temperature combination 2 ≥0.98), respectively. All colour parameters colour All respectively. ≥0.98), 3 k. The crust and crumb moisture contents -7

to 5.98x10 -7

m 2 min -1 w withincreaseinair 0.30 -0.70)ofthe 2 /s and 3.09 to 2.00 /s and 3.09 to 2.00 2 =0.99). The gulab o w C. C. < price ranged from Rs.18-30/litre in the region while price rangedfromRs.18-30/litre intheregionwhile 2-5 dairy animals (64-76%). The milk procurement possessed respondents the of Majority (56-76%). marginal farm holding of less than one hectare with (62-70%) experience farming of years 10-20 with (54-68%), middle-aged were respondents small dairy holders revealed that majorityof the of profile socio-economic and socio-personal The dairy production systems in thesouthernregion. region andtostudytheconstraintsofsmallholder studies ofsuccessfulsmalldairyfarmersthe areas of southern region, to analyse selected case holder dairy production systems intheselected small of profile the study to objectives specific with up was taken region southern systems inthe The profile study on small holder dairy production Systems inSouthernRegion A Profile Study of Smallholder Dairy Production extract. peel orange with incorporated ghee in flavor and panelists didnotobserveanyundesiredcolour in ghee.Theresultswereencouragingas sensory observed changes flavor and color and prepared powder wasalsoincorporatedintocream,ghee orange peelpowder.Solventextractof (peroxide value,TBA and conjugateddienes)of get ghee.Gheewasanalysedforantioxidanteffect powder was incorporated to butter and clarified to for furtheruse.Onetotwopercentorangepeel peel, packedinLDPEandstoreddeepfreezer 60°C. Powderwaspreparedfromdehydrated was dehydratedatanoptimumtemperatureof flavor of ghee were conducted. Orange peel from orange peel and their effect on color and Preliminary studiesonextractionofantioxidants identified. were seeds grape and waste tomato products such as orange peel,pomegranate antioxidant BHAwithnaturalantioxidants.By- undertaken withanintentiontoreplacesynthetic extracts offruitandvegetableby-productswas The projectonextensionofshelf-lifegheeusing Fruit andVegetableBy-products Extension ofShelf-lifeGheeusingExtracts adsorption anddesorption. the dieteticsamplerecordedsimilarvaluesfor higher thanadsorptionforthecontrolsamples, isosteric heatandentropyduringdesorptionwas computed relative to its moisture content. The Thermodynamic properties oftheproducts were 439.82 J/kgwaterat20,30and40 7.40, 7.06,2.99unitsand622.35,1008.67,904.85, hysteresis unitsandenergywereestimatedat4.6, The 80%. - 70 between varied and loop hysteresis –– Southern Regional Station,Bangalore –– o C, respectively.

73 ndri annual report 2013-14 74 ndri annual report 2013-14 like, clean milk production practices, fodder programmes on dairy production technologies Theoff campustraining activity. dairy based SHGs expressedtheirwillingness totakeupthe motivated tostartdairybased activitiesandtwo village level meetings and group members were in doingdairybasedactivitieswerediscussed at andconstraints andtheirexpectations activities Finance Group.Thepreliminarysurveyabouttheir Center No143,promotedbySamasthaMicro promoted byPeoples Trust NGOand Group were BhuvaneshwariGroupatSuradenapura, with localNGOs.ThetwowomenSHGsselected Rural DistrictofKarnatakaState,inassociation groups from two different villages of Bangalore SHG, the project team has identified the two SHG year, inordertoestablishthedairybasedwomen interventions. Aspertheplanofworkforsecond institute also dairyprocessingactivitiesthrough and practices farming dairy scientific in groups two successfuldairybasedwomenselfhelp The projectisprimarilyfocusedondeveloping Women: AnActionResearch Entrepreneurship Rural Dairy Developmentof dairy developmentintheregion. to fillip a provides and problem the alleviating technological andgovernmentalinterventionfor of farm labour, etc.This indeed warrants suitable productivity of dairy animals, non-availability non-availability ofgreenanddryfodder,low milk procurementprice, high cost of cattle feed, enveloped acutewatershortage,non-remunerative constraints indairyfarmingtheregionprimarily The constraint analysis revealedthatthemajor experienced bytheselectedrespondents. study area, which traced the historyof dairying as selected successful smalldairyhouseholds in the The casestudyanalysiswasdonefromthetwo per dayintheregionrangedfrom 6.0-8.5 kg/day. Rs.17-23/litre Theaveragemilkyieldperanimal/ cost ofmilkproductionintheregionrangedfrom the milksalepricerangedfromRs.30-35/litre.The –– Southern Regional Station,Bangalore –– Successful DairyWomanwithKamadhenufromKolar, Karnataka knowledge about dairy farming with favourable knowledge aboutdairyfarming withfavourable had mediumlevelofextension participationand that amongtherespondents mostofthefarmers average of fouranimals per household. It was found land holding and a medium herd size with an and mostofthedairyfarmers possessedmarginal most of the respondents were in middle age group villages. Fromtheresultsitwasconcludedthat respondents wereselectedfromeachofthefour in entrepreneurshipofdairyfarming.Thirty the constraintsexperiencedbydairyfarmers entrepreneurship ofdairyfarmersandtoidentify influencing factors the analyse to farmers, dairy of profile psychological and economic personal, The studywasundertakentoanalysethesocio- Nadu Tamil in theNorthernDistrictsof Farmers Dairy of Profile Entrepreneurial on A Study board fordairydevelopmentintheregions. organized marketing facilities would provide spring network, othersupportingservicescoupledwith to milkproductionintheregion.Betterextension fillip give would which suitably, addressed be to and constraintsfacingthefarmingcommunityneed production areyieldingpositivereturnsintheregion positive netreturns.Crossbredandbuffalomilk cows, and for crossbred cows and buffaloes it was (LC &B).Thenetreturnswerenegativeforthelocal labour cost ranged between 24% (CB) to 30% percent of share The (CB). 64% to (B) 58% from varied cost and cow andbuffaloewascomputedat` production perlitreofmilk,forlocalcow,crossbred net returns.Inthecaseofhillregion,costmilk while, crossbredcowsandbuffaloesyieldedpositive The netreturnswerenegativeforthelocalcows, (CB). 29% to (B) 21% between ranged cost labour of cost varied from 61% (LC) to 64% (CB & B). The share ` and buffalo(B)wascomputedat` litre ofmilk,forlocalcow(LC),crossbred(CB) district. Inplainregion,costofmilkproductionper sample forthestudyfromplainandhillregionsof Nainital districtand100farmhouseholdsformedthe systems.The study was carried out during 2012-13 in Uttarakhand and it is closely associated with farming ruralmountainous areasofactivities inthe Dairy farmingisoneoftheimportanteconomic Nainital DistrictofUttarakhandState Economic AnalysisofMilkProductionin two SHGmemberswerecollectedandanalyzed. taken at present. The socio-economic profile of the the entrepreneurshiptrainingpackagesarebeing mobilizing theSHGmembers,preparationsfor etc. wereprovidedtothebeneficiaries.After cultivation, scientificanimalrearingpractices, 27.19, respectively. Share of Feed cost in the total 27.19,respectively.ShareofFeedcostinthetotal ` 26.88, respectively. Share of feed cost in the total 26.88,respectively.Shareoffeedcostinthetotal 27.61,` 26.47,` 19.17 and 19.17and 19.23 19.23 , blemkar e.g., 4 quintalleafpertreeyear). Freshtreeleaves biomass yield wasobserved in Maartrees(3.5to to 0.3quintalleafpertree year)andhighest tree(0.2 per treeyear)wasobserved inblemkar level (msl).Lowestbiomass yield (meanleaf located about4,000to10,000ftabovemeansea evaluated. Thesetreesaregenerallygrown/ collected and their nutrient compositions were spp.) (Symplocos cratagoides), (Symplocos ngeksingh walliasehiana), (Quercus phrengpa myrsinites), (Lingustrum humboltiana), (Salyx asitica), (Buddija blemkar are commonlyfedtoyakse.g., Some ofthelocallyavailabletreeleaveswhich provide good wood as well as used as fire woods. alsonot onlyusedforlivestockfeeding,but with various species of treefodders, which are In ArunachalPradesh,theforestsareendowed Leaves ofNorth-EasternIndia Manipulation ofRumen Fermentation usingTree production technologies. producers haveknowledgeofdifferentdairy milk non-member % 63.0 and members % 74.0 compared tothatofnonmembers (22.62g).Overall was observedhigher(49.37g)onmembersas consumption milk percapita Average state. the through intensivedairydevelopmentprogramsin Jharkhand (2milkinganimals)whichrequiredhelp incaseofof milkinganimalswereobserved by WestBengal,Bihar.Whilelowestnumber as comparedtononmemberhouseholds followed and Sikkim(4-5animals) on member households estimated andfoundhigherincaseofNagaland families. The stocking rateof milking animals was of dairyco-operatives onmemberhousehold of theEastern/NEH regionforimpact analysis Bihar, Orissa,Jharkhand,Sikkim,andNagaland) Bengal, (West insixstates wasconducted The study NE RegionofIndia on the Economy ofRuralHouseholds in Eastern/ Impact AnalysisofWomenDairyCooperatives The overallentrepreneurialbehaviourindexamong confidence. self and cosmopoliteness behaviour, ability, planninginformationseeking co-ordinating orientation, risk ability, making innovativeness, achievementmotivation,decision selected attributesofentrepreneurshipviz., behaviour indexamongtherespondentsto indicated medium level of entrepreneurial Thestudy enterprise. dairy towards attitude and domkar (Quercus fenestrate) (Quercus matekpa , domkar (Symplocus racemosa), (Symplocus (Costanpsis sp.), (Costanpsis maar , salyx E (Berberis spp.), (Berberis baggar astern maar, (Spiral maarma , zimbu khamsuma, R had been egional baggar, zimbu salyx

S phrengpa, NH from 1.8to9.7mEq/dlincubationmedium. vitro gasproductiontest.TVFAvaried 32.7, 28.3,27.8and31.8ml/gleafDMunderin myrsinites) (Lingustrum and zimbu humboltiana), (Salyx salyx asitica), (Buddija blemkar of production methane vitro In %). (60.2 sp.), was observedinmaar(Costanpsis 24.1 % on DM basis respectively. Highest IVTDMD cellulose contentvariedfrom45.6to57.9and13.2 and NDF as where % 15.1 to 11.3 and 96.1 to 88.9 OM andCPcontentoftreeleavesvariedfrom 48.8, 33.1, 31.8, 39.3 and 44.6 % DM on fresh basis. matekpa contained26.7,40.3,23.9,32.8,36.6,45.7, cross (41%) followed by 2-breed (33%) cross. The cross. (33%) 2-breed by followed (41%) cross revealed thatmajorityofanimals fallsin3-breed composition ofanimals present study,thegenetic of different geneticcombinations/groups. In the with respecttolactationtraits ofcrossbredcattle evaluate andcomparetheproduction performance and phenotypic parameters of lactation traits and to genetic to estimatethe factors onlactationtraits, used tostudytheeffectofdifferentnon-genetic animals extendedover39years(1974-2012)were crossbred Jersey 274 of lactations thirteen to first Data on731lactationrecords which comprises of at ERS,NDRI,Kalyani Evaluation ofLactationTraitsCrossbredCattle livestock inArunachalPradesh were thebesttreeleafs/fodder forfeedingtothe salyx (Salyx humboltiana) andmaar (Costanpsis sp.) concluded from the preliminary x 10 Number oftotalrumenprotozoavariedfrom22.5 and theabsenceofPolyplastron multivesiculatum. type populationduetopresenceofEpidinium mediumwasBrumen liquorandincubation in vitro.Rumenprotozoapresentthecollected myrsinites) leaf, respectively with incubation media sp.) (Costanpsis maar racemosa), (Symplocus asitica), (Buddija incubation ofblemkar was 12.3, 15.1, 14.7, 13.8 and 14.2 mg/dl due to faced bythefarmersinstudyarea. of landforgrazingwerepredominantconstraint remunerative priceformilkandnonavailability of farmingenterprise.Highcostcattlefeed,non milk yield, extension participation and knowledge annual income,landholding,livestockpossession, dairy farmingexperience,socialparticipation, viz.,education, independentvariables with the dairy farmers was positively and significantly related tation 3 3 -N concentration in the incubation medium to28.1x10 , K khamsuma, ngeksingh , alyani 3 permlincubationmedia.Itwas (Symplocus racemosa), (Symplocus domkar (Salyx humboltiana), (Salyx salyx (Lingustrum and zimbu (Costanpsis sp.) (Costanpsis maar studies that in vitro studies that leaf maarma tree leaves was 30.5, domkar and sp.

75 ndri annual report 2013-14 76 ndri annual report 2013-14 correlations ofTMYwithLLwerealsohigh(r 41 to0.99).Similarly,thegeneticandphenotypic LL ranged frommedium to moderatelyhigh(0. and geneticcorrelationsofMY305DwithTMY lactation traitsofcrossbred cattle. Thephenotypic appropriate geneticmodelforanalyzingthe environmental effectduetodam,wasthemost includes directgeneticeffectandpermanent that animal model.Hencetherepeatabilitymodel, to estimatesobtainedbysiremodelandsimple lactation traitsweremorepreciseascompared estimates for model, theheritability repeatability ignorance of maternal effects of dam, however, in of animals by sire model were biased due to that heritabilityestimates forlactationtraits respectively underthismodel.Thisstudyrevealed LL were 0.42±0.05, 0.42±0.06 and 0.13±0.07, heritability estimatesforMY305D,TMYand model. However,inrepeatabilitymodel,the and 0.19±0.05,respectivelyundersimpleanimal 0.45±0.05 0.49±0.04, were figures correspondence respectively undersiremodel,whereas,the animals were0.65±0.14,0.54±0.13,and0.25±0.09, estimates forMY305D,TMYandLLof heritability appropriate modelforlactationtraits.The the modelswerecomparedtoselectmost animal modelandrepeatabilityall siremodel,simple by animals wereestimated The heritabilitiesofMY305D,TMYandLL covariate forTMYofanimals. linear as (P<0.01) significant statistically found length ofanimals.Dayslactation(milking)were lactation on effect (P>0.05) non-significant had calved indifferentseasonsbuttheseasonofcalving 305 days milk yieldand TMY existed amongcows in differences (P<0.01) Significant animals. of LL and TMY MY305D, on effect (P<0.01) significant (P>0.05) forLL.Theperiodofcalvinghadhighly of animals but the effect was found non-significant had significant (P<0.01) effect on 305DMY and TMY 403.02±9.24 days.Thelactationnumberofanimals kg, respectivelywithaveragelactationlengthof animals were 2727.70±52.74 and 3306.36±68.57 (MY305D) andtotalmilkyield(TMY)ofcrossbred overall least-squaresmeansfor305-daysmilkyield –– EasternRegional Station,Kalyani –– 0.69, r g = 0.71)andsignificant. p = when greenfodderavailabilityisscanty. for theimprovement inmilk yield especially where/ to serveasagreensupplementforthedairycattle Fresh better feedconversionefficiency. treatment group thanincontrol group indicating the in lower (P<0.05) significantly was yield FCM milk constituents.Totaldrymatterintakeperkg in change significant any without 12.5% by yield FCM and 11.2% by yield milk in increase an caused T0 andT1,respectively.SupplementationofAzolla average valuesbeing7.04±0.15and7.92±0.09for milk (FCM)yieldalsoshowedsimilartrendwith in T1(7.14±0.08)thanT0(6.42±0.13).Fatcorrected (P<0.01) higher significantly was fortnights 6 over conventional ration.Theaveragemilkyield(kg/d) group (T1) 2kg of fresh Azolla recommendation ofNRC2001.Inthetreatment straw, greenfodderandconcentratemixtureasper group (T0) werefedonconventionalrationofpaddy a lactationtrialof90days.Theanimalsincontrol supplementation incrossbredcattlewasstudied nutrients. TheeffectoffreshAzollamicrophylla (210.7-296.7 gkg indicated that it was a good source of plant protein around 200-230g/sqm/day.Chemicalanalysis fecourse forlivestock.ThefreshyieldofAzollawas Azolla Dairy CattleRation Utilization ofSome Newer Feed Resources in Comparative Nutritional Evaluation and optimum productivityinthisagro-climaticregion. the forobtaining genotype suitable best the be ½ JerseyxTharparkar among allcrosses and therefore, theanimals having (2495.35±92.44 kg)andTMY(2948.68±121.01 305DMY lowest produced inheritance Jersey 50% other genetic groups. The animals having less than (3636.09±66.86 kg)ascompared toanimalsof milk (2981.65±51.14kg)andtotalyield Tharparkar crossesproducedhigher305-days the differentcrosses, the half-bredofJerseyand of animals.Among study exceptlactationlength under traits lactation the all on influence (P<0.01) The geneticgroupofanimalhadsignificant was found to have potential Azolla microphyllawasfoundtohavepotential was evaluated as alternative feed microphyllawasevaluatedasalternativefeed −1 DM) andfairsourceofother genetic combination may was supplemented over was supplemented over

3 conducted inadoptedvillages. infertility and veterinary aid campaigns were maturity andtominimize inter-calving interval, A.I. usinghighpedigreebulls.Toreduceageat and selective breedinginlocalbuffaloesthrough animals, cross-breeding wascontinued incows In ordertoupgradetheexistingbreedsofdairy ● ● ● ● carried outthroughtheseCentersare: activities of thedivision.Themajoractivitiesbeing Karnal andkeepstherecordsofallextension villagesaround adopted inthe work extension of Dairy Extension Division provide a base for The Field/FarmTechnician(FFT)Laboratory Field/Farm Technician(FFT)Laboratory provide thespotsolutionsto theirproblems. to updatetheknowledgeof dairy farmers and to three sangosthieswereorganizedatvillage Fifty Kissan Sanghosthies performance ofdairyanimals to improvetheproductiveandreproductive were alsoconducted.Specialattentionwasgiven ailments. Ecto-endo-parasite control programmes reproductive disorders and various Veterinary Camps were held and 3796 cases were treatedfor During theApril, 2013 – March, 2014, 61 Veterinary Infertility andVeterinaryAidCampaigns Activities ConductedinAdoptedVillagesfrom 7 6 5 4 3 2 1 Sr. N.

practices. of breeding,feedingandimprovedmanagement To educate farmers regarding scientific methods diseases. contagious against vaccination provide To To provide necessary treatment to the animals. campaigns. aid veterinary and fertility organize To TRANSFER OFTECHNOLOGY General treatment No. ofbuffalocalvesborn No. ofcross-breedcalvesborn Conception rate(%) A.I. inbuffaloes Conception rate(%) A.I. incows Activities conducted April, 13–March,2014 414 256 423 30.67 917 38.33 1769 No. ofcases D airy E xtension by NDRI. and apprisingthemaboutforinnovationsmade the visitorstofacilities programme bytaking visited NDRI.Extensiondivisioncoordinatedthe from 104Colleges/Institutions/Universities A totalof6121visitors(studentsandfaculty) 2014) Educational VisitsandTours(April13toMarch developed dairytechnologiesoftheinstitute. Samachar madethefarmerswellawareofnewly in differentstatesthroughoutthecountry.Dairy to dairy owners and other dairy entrepreneurs farmers,asaregularpractice the to distributed NDRI, Karnal.Dairy Samachar was sent/ and publishedbyDivisionofDairyExtension, Quarterly DairySamachar were compiled, edited Dairy Samachar constraints facedbythem. the practicesadoptedbyfarmersandalso their door,andthescientist couldunderstand technologies amongfarmingcommunity at dissemination of dairy production and processing linkages. This programme facilitated theeffective and identifiedthevillageasperfunctional Extension Scientist inthe team acted as coordinator selected villagesoneverysecondSaturday.The nearby visited groups andmanagement processing scientists consistingofspecialistsfromproduction, continued intheyear2013-14.AteamofNDRI Education at Farmers’ Door” initiated by NDRI The innovativeExtensionapproach“Dairy Dairy EducationatFarmers’Door(DEFD) programmes. the feedbackwasalsocollectedonextension day toproblems.Duringthese sessions their opportunities to the farmers to find the solutions to in these sessions which provided excellent Question/Answer sessionswerealsoarranged

77 ndri annual report 2013-14 78 ndri annual report 2013-14 the ruralareas. poor in poorest amongstthe for the opportunities economic statusoffarmersandcreatejob production andalliedactivitiesforimproving The main aim of KVK is to accelerateagricultural main targetgroup,arealwayskeptinmind. training and(iii)weakersectionofthesociety– work experience – the main method of imparting viz., (i)agriculturalproduction–theprimegoal,(ii) “Learning byDoing”.Threefundamentalprinciples based skillorientedtrainingprogrammesthrough Kendra has developed infrastructure to runtheneed became operationalinJuly1976.Subsequently,this Krishi VigyanKendra (KVK)atNDRI,Karnal museum site. one varietyofTurnipwerecultivatedatforage varieties of Berseem, two varieties of oats and Seasonal foddercrops:inRabiseasonthree museum. paragrass-1, Bahiagrass-1)wereplantedatfodder grasses (napier-7, Guinea-3, Nandi grass-1 Anjan-3, Perennial Grass:Presently16varietiesofperennial fodder productionmoduleshavebeendeveloped. was establishedatNDRIinwhichfollowingfour availability roundtheyear,a2acrefoddermuseum Fodder Museum:To maintain thegreenfodder Sponsored AtKVK Dairy Production On Campus Title ofthecourse Sponsored AtKVK Dairy Processing Crop Production Sponsored AtKVK Vermiculture Bee-keeping Fish Farming Home Science Horticulture Sponsored Total (A) Short IntegratedTrainingProgrammes (B) Total (A+B) Training ProgrammesOrganizedbyKVK(April,2013toMarch,2014) K rishi V igyan Duration (days) 1-14 3-5 4-5 1-2 5 5 3 3 3 4 4 5 K agencies andlinedepartments. government, non-governmentandvoluntary the KVKdeveloped close linkages with different moreeffective, programmes training making and terminatedindiscussionassembly.For farms, livedemonstrationunits,workshops trainings startedwithfieldunits,different economically viableforthefarmers.The enterprises makethe to consideration into entire conceptoffarmingsystemwastaken undertaken. Whiledesigningthecourses, training, followupextensionprogrammewas designed fordifferenttypesofclientele.After At KVK,needbasedtrainingcourseswere farming practices. technologies oftheinstituteandmoderndairy latest queries regarding particular /general their departments all over the countryinresponse to services to individual farmers / NGO’s / relevant services through postal, e-mail and telephonic The ExtensionDivisionprovidedregularadvisory Advisory Services in ensuingkharifseason. planted :100lemonplantshavetobe Horti-pastrol planted inensuingkharifseason. Silvi-pastrol :10speciesoffodderhavetobe endra No. ofcourses (KVK) 111 205 66 13 94 9 4 7 2 1 3 3 2 1 beneficiaries No. of 1968 3272 3026 6298 568 122 254 56 99 51 30 65 39 20 Mandays trained 15541 18832 9776 2735 1069 3291 266 495 153 195 506 156 100 90 is to conduct FLD in various crops to generate this region.OneoftheprimemandatesKVK production oftheoilseedandpulsecropsin Programme topromoteandpopularizethe Front LineDemonstration(FLD)isaNational OILSEEDS &PULSES(2013-14) FRONT LINEDEMONSTRATIONS(FLD)ON (Revenuegeneratedfromsponsoredcourses= • Extension ActivitiesofKVK • • Dairy Production Off Campus S. No. 1. 2. 3. 4. 5. 6. Dairy Processing Crop Production Vermiculture Home Science Total (B) Grand Total(A)+(B)+(C)

Insemination 1824calveswereborn. Artificial Through animals. dairy in A.Is. 3583 2796forpregnancydiagnosisand dehorning, treatment, 136for treatment, 115ofinfertility attended, outofwhich128caseswereforgeneral Karnal. Inthesecentres12611caseswere Gate, Taprana, Kailash,&PhusgarhandJundla activities intheadoptedvillages,namely: management health animal out carried KVK as toincreasethesoilfertility. pulse aswell grow summerMoongforgetting categories of land holding wereencouraged to promoted andthefarmershavingdifferent were MH-421 and SML-668 varieties Moong and alsotothefarmersofother states. of farmersandfarmwomen theKarnaldistrict production wasdemonstrated tovariousgroups crop Wheat in technology Tillage Zero Crop Mustard Gram Summer Moong Jowar Fodder(multicut) Maize Fodder Paddy ` 21,23,426/-) Varierty CS-56 CS-54 Pusa Vijay BG-1103 MH-421 Sudax Chari-1 African Tall PUSA-1121 PB-6 (1401) 1-2 1 1 1 1 • during 2013-14. Following FLDprogrammewereconducted of newlydevelopedcropproductiontechnology. production potential yield, andalsotoprovethe to studythefactors,whichenhanceoptimum production data and feedback information and • •

“National Nutrition Week”’ (1 feeding forinfants. theme ofthedaywas the importanceof breast 2013 inwhich 62 ruralwomen participated. The KVK was held on 27 of committee Advisory Scientific of meeting A and thelocalICARInstitutesparticipated. In this meeting officials of the line departments and actionplanfortheyear2014wasproposed. the progressreportofKVKwaspresented chairmanship of Director, NDRI Karnal inwhich in villageKailashDistt.Karnalon 05 KVK organized World Breast Milk Feeding Day production. women andcleanmilk children andpregnant nutritional dietsparticularly foradolescent were educatedaboutthelow cost homemade farmers andfarmwomenparticipated.They 2013) wasorganizedinKVKwhich85 Total No.ofDemo. 230 13 25 10 16 16 12 4 1 6 1 6 8 8 4 3 th December, 2013 under the 6874 363 576 90 16 93 14 Area (ha.) 3.23 3.22 1.61 1.21 –– KrishiVigyanKendra (KVK)–– 4.0 2.0 3.0 6.0 2.0 st -7 th September Av. yield 19468 th 423 636 August, 90 16 93 14 16.47 16.42 17.36 19.83 531.0 508.0 55.57 7.28 48.0

79 ndri annual report 2013-14 80 ndri annual report 2013-14 Services RenderedinAgriculturalTechnologyand ProductsfromApril,2013toMarch,2014 • • • Mandate ofATIC and alliedfields. and to havelatestinformationrelateddairying facilities ofATIC fortheorientationprogramme users, largenumberofstudentsareavailingthe extension workers visit this centre. Besides end number of entrepreneurs, practicingfarmers and centre for the end users visiting this centre. Large institutions locatedatKarnalareutilizedbythis institutes, researchstationsofICARandstate information availablefromotherresearch relevant NDRI, from information Besides fields. information ondairyingandalliedagricultural 2004. This centre is engaged indisseminating NDRI Karnal became operational in November at Centre Information Technology Agricultural • • • 1 No. S.

end userstotheresearchsystem. the from feedback for mechanism provide To problem solving. adopting amultidisciplinaryapproachto by services advisory farm the strengthen To to theclientele. Institute withaviewtodeliverqualityservices and technologiesdevelopedbytheResearch information aswellproducts for agricultural system delivery window single a provide To National DairyMela thatwas held on25 organizing in role important an played KVK direct seedingofrice. experiments andfrontlinedemonstrationson South Asiaprogrammeorganizedvarious for Initiative System Cereal the under KVK other states. the Karnaldistrict and also to thefarmers of various groups offarmers and farm women of cultivation ofwheatweredemonstrated to in technologies agriculture Conservation 27 including progressivedairyfamers, th February,2014.Morethan14000visitors technology products agricultural Guidance of Services/ Transaction A gricultural person oftheATIC sectors with the responsible Personal contactbytheservice Process T echnology th - I nformation • • 7. 6. 5. 4. 3. 2. 1. in disseminationofinformationtoitsusers: Presently ATICNDRIisusingfollowingmethods Dissemination ofTechnologicalInformation 48,65,782/. activities duringApril,2013toMarch,2014was` Total revenuegeneratedatKVKthroughvarious REVENUE GENERATED •

1200 days standard Service

25 on occasion of Innovationand Seed Dayand on education. and practices,farminputsagricultural information pertainingto farming skills agricultural of repository a as function To stakeholders inthestate. different the to services consultancy offer To Mela organizedbyNDRI,Karnal. Karnal; on15 2013 onFoundationDayatCCSHAU,Uchani, KVK organized exhibitions on 16 on exhibitions organized KVK country participatedintheMela. entrepreneurs and farm women across the th etc. varieties, area specific mineral Providing materialinputslikeimprovedseed Providing Publications. number (1800-180-1199) Information through toll free telephone Visits todairyfarm Audio/Video shows participating inMelasandExhibitions. Display ofmodels,etc;organizing/ Personal interactionwithvisitingfarmers. -27 th February, 2014 during National Dairy 2014 duringNational February, reported Year attended duringthe *No. ofsuch services C th October,2013atD.W.R.,Karnal entre 8802* (ATIC) beyond 100days services pending No. ofsuch supplements, th September, NIL * DetailofNo.Services BOOKS Sale ofSeeds&booksthroughATIC(April2013-March2014) 1. Sr. No. 1. Total 9. 8. 7. 6. 5. 4. 3. 2. 1. Seed ProductionUnit(RFS) SI. No. 2. 3. 4. 5. 6. Total lecture Dairy/Agriculture related information through video show and Detail ofservices Personal discussionwithsubject-matter-specialistondairyfarming Information throughdairy/agricultureliterature Information onseed/fertilizer/compost etc. &dairying (Toll-free)onagriculture telephone Information through Information throughe-mailonagriculture&dairyingetc. Sh. ArvindR.Kaushal,Secretary,ICAR &AdditionalSecretary,DAREdeliveringConvocationAddress Grand total Number ofbookssold:1646Books Vermi Compost FYM (Packets) FYM (Loose)inQuintal Paddy (Grain) Paddy (Grain) Mustard seed Mustard (Grain) Oats Turnip Paddy Name ofcrop at NDRIon14 - - - P-1121 Pusa-44 Chinese cabbage Chinese cabbage Kent - Pusa-44 Variety th February,2014 05 Pkt. 1736 220.00 750.00 340.00 35.00 121.00 4140.00 02.00 8390.00 Quantity (kg) - No. ofServices* –– Agricultural Technology Information Centre (ATIC) –– 2401 5681 8802 179 134 395 12 50.00 34720.00 3880.00 18750.00 4250.00 525.00 6050.00 124200.00 300.00 289960.00 Amount (Rs) 482685.00 645064.00 162379.00 No. ofPersons 15790 7167 2401 5681 134 395 12

81 ndri annual report 2013-14 82 ndri annual report 2013-14 4 regulations ofICAR. rates inaccordance withtheprescribedrules and awarded Institutescholarship atthefollowing Masters inDairyingand Ph.D. studentsare Scholarship andFellowships 2014-15. Husbandry at Eastern Campus, Kalyani from The InstituteisgoingtostartDiplomainAnimal Campus,Bangalore. atSouthern management processing equipments and dairy business and dairyproducts,engineeringaspects of dairy in dairy processing and quality control of milk The Diploma programme offers intensivetraining Husbandry Diploma inDairyTechnologyandAnimal 2014-15. Gynaecology disciplinefromthe academic session Agronomy (Forage Production) and Vet. Obst.& Production). The Institute is going to start Ph.D. disciplinesexceptAgronomy(Foragethe above The InstituteoffersDoctoralprogrammesinall Assurance" fromacademicsession2014-15. & Safety and M.Sc."FoodQuality Gynaecology) Reproduction, VeterinaryObsterics & The instituteisgoingtostartM.VSc.(Animal and xiii)Agronomy(ForageProduction). xii) Agricultural Extension/Veterinary Extension Economics; Economics/Livestock Agricultural xi) ix) AnimalNutrition;x)Physiology; viii) LivestockProductionandManagement; Biotechnology; vii)AnimalGeneticsandBreeding; Engineering; v)AnimalBiochemistry;vi) ii) DairyChemistry;iii)Technology;iv) the followingdisciplines:i)DairyMicrobiology; The Institute offers Master’s degree programme in Master’s andDoctoralDegreeProgrammes processing plants. and milkproducts;engineeringaspectsof in processingandqualitycontrol ofmilk training This 4yeardegreeprogrammeoffersintensive B.Tech. (DairyTechnology) on differentaspectsofdairying. provide broadbaseaswellspecializedtraining 2013-14. Thecourses have beensodesignedasto Deemed Universityduringtheacademic session The followingcourseswereofferedbyNDRI Academic Programmes DAIRY EDUCATION

Ph.D. (In-service) Ph.D. Ph.D. ICAR SeniorResearchFellowship Master’s degree ICAR JuniorResearchFellowship Ph.D. (In-service) Ph.D. Master’s degree Institute Scholarships Scholarship. Memorial Scholarships and Prof. R. D. Verma P. BhattacharyyaMemorial Trust,M.Malik Memorial, AlumniSilverJubilee Scholarship,Dr. Dr.I.S.Verma Trust, and SushilaBenMehta Memorial, Dr.P.G.NairAward,Bhogi Bhai Dr. L.C. Sikka Memorial Trust, Dr. D. Sundaresan and individualdonorsi.e.M/sNestleIndiaLtd., A number of scholarships are offered bycorporate state ofhis/herdomicile. is locatedoutsidethe the university/institute that per month is awarded by ICAR on merit provided The NationalTalent Scholarship(NTS) @` National TalentScholarship and ` 1000/-permonthfor3years as contingency. year; and ` year and` veterinarians) for1stand2nd ` as contingency. year; and10,000/-perannum year and` veterinarians) for1stand2nd ` 12,000/-permonth(fornon annum ascontingency. for twoyearsand`6,000/-per per month (for veterinarians) veterinarians) and` ` annum ascontingency. three yearsand`10,000/-per ` annum ascontingency. three yearsand`10,000/-per ` as contingency. years plus`6000/-perannum ` contingency. 8,640/- per month (fornon 8,640/-permonth 7,560/-permonthfortwo 14,000/- per month (for 10,500/-permonthfor 1,000/- per month for 1,000/-permonth ` 10,000/-perannumas 10,000/- per annum 10,000/- perannum 15,000/- for3rd 14,000/- for3rd 12,000/- 1,000/- 1,000/- 2013-14 was conducted by NDRIon10 by 2013-14 wasconducted for admissiontoPh.D.programmethesession An AllIndiaCompetitiveEntranceExamination Entrance Examination respectively atNASCComplex,NewDelhi. • major functionsoftheCellareasfollows: opt for higher studies in India and abroad. The addition to employment a number of students also ranges from Rs. 20,000 to 60,000/- per month. In (Govt./Cooperative/Multinationals). Salary are gettingemploymentinDairy/FoodIndustry campus interviews. Passed out studentsofNDRI employment inreputedorganizationsthrough Master inDairyingstudentswereprovided University. B.Tech.(DairyTechnology)and out students in various disciplines of the Deemed training andplacementservicesforthepassing Cellprovidescareer guidance,The placement Career Guidance,TrainingandPlacementCell • • • • of ICARon15 programme was held by theEducation Division Counselling foradmissiontoUG and PG Counselling forAdmissions was closedon30 programmes weremadeand theadmissionprocess Technology), M.Sc./M.V.Sc./M.Tech. and Ph.D. Diploma in Dairy Technology, B.Tech. (Dairy Admission for theacademic session 2013-14for Admissions i.e.Karnal,BangaloreandKalyani. centers three at

students incareerplanning. To counsel the undergraduate and post graduate processing. and food area ofdairying in the level engaged academic bodies atthenational and international and corporate of directory a compile To screening withprospectiveemployers. placement/ facilitating for students year final the of resume of compendia a prepare To by arrangingcampusinterviews. postgraduate studentsfromvariousdisciplines To evolve mechanism for placement of graduate/ community andindustry. to maintaincloserliaisonbetweenstudent To arrange seminars/workshops/presentations th , 27 th August,2013. th , 30 th Juneand1 st th July,2013, May, 2013 May,2013 in Master’sandPh.D.degree programmefor Merit certificatetotopperofeachdiscipline performance incoursework werealsoawarded. students inB.Tech.(DT)Programme basedon out pass total the of 20% to Merit Certificate overall performanceinCoursework. Director’s Gold,SilverandBronzeMedalsfor Master’s andDoctoralprogrammeswereawarded Three toppersstudentseachinB.Tech.(DT), Report. Deemed UniversitypresentedtheConvocation A. K.Srivastava, Director & Vice Chancellor, NDRI Research delivered theConvocationAddress. Dr. Kaushal, Secretary, Indian Councilof Agricultural Degrees Awardedduringthe12 the Ph.D.Programme. students includingthreeforeignnationalsjoined foreign national,joinedMasters’programme104 Technology), 129whereasstudentsincludingthree Technology, 29studentsjoinedB.Tech.(Dairy A totalof27studentsjoinedDiploma inDairy • Meetings Ph.D. Dairying in Master B.Tech. (DairyTechnology) • • • was held on 14 Twelfth Convocation of NDRIDeemedUniversity Twelfth ConvocationofNDRIDeemedUniversity •

Affairs washeldon11 Committee on Course Curricula and Academic 74 was heldon30 Scholarship, Financial andAcademic Progress held on28 andDisciplinewere Faculty, StudentsProblems 35 30 39 2014. 12 36 28 52 nd th th th th th th th January,2014. February,2014. meetingofAcademic Council washeldon October,2013. MeetingofAcademic Council washeldon , 53 , 75 meeting ofStanding Committee on rd th meetings of the Standing Committee on meetingsoftheStandingCommitteeon and76 th September,2013and30 th February, 2014. Shri Arvind R. th January,2014. th meetingoftheStanding th , 25 th October,2013and th Convocation : : : –– DairyEducation th January, 59 128 39

83 ndri annual report 2013-14 84 ndri annual report 2013-14 certificate. an amountof` for PerformanceEvaluation”. The award carries Sciences for Justifying Existence :A Key Issue topic “UsefulScienceVersus 2014 onthe January, Bandopadhyay deliveredthelectureon28th on Dr.S.K.Bandopadhyay,Member,ASRB. Dr. K.IyaOrationaward-2014wasbestowed Dr. K.IyaOrationAward-2014 and acertificate carries anamount of` Food in RelationtoHumanHealth”.Theaward February, 2014 on the topic “Environment and ICMR. Dr. Katoch delivered the orationon10th bestowed onDr.V.M.Katoch,DirectorGeneral, Award-2014been Oration Dr. N.Dastur Dr. N.DasturOrationAward-2014 20,000, acitation,shawlandcertificate Agriculture”. Theawardcarriesanamountof` 2014 onthetopic“YouthforTransormingIndian Ayyappan delivered the lecture on 11th February, DARE andDirectorGeneral,ICAR,NewDelhi.Dr. Secretay, onDr.S.Ayyappan, was bestowed Dr. D.SundaresanMemorialLectureAward-2014 Award-2014 Dr. D.SundaresanMemorialLecture theses ineach programme wererecommended Two Certificate. and Citation Medal, Gold carries Management Group were awarded. The award Ph.D. Programme of Production,Processing and Gold Medalsforbestthesisresearchworkin Best Master’sThesisAwards Groups) carrying a citation, a certificate and each inProduction,ProcessingandManagement Best ThesisAwardsforMaster’stheses(one Best ThesisAwards criteria werealsoawarded. eligibility performance incourseworkasperthe –– DairyEducation Production Social Science&Management Processing Group Production Social Science&Management Processing Group 20,000,acitation,shawland a 20,000,acitation,shawl Mr. DineshKumar Mr. SanjitMaiti Mr. ApurbaGiri Name oftheStudent Mr. DevPriya Mr. RanjithKumar Ms. SariT.P. Name oftheStudent Kundli, Distt.Sonepat,Haryana. Technology Entrepreneurship and Management, of Food of mutualinterestwithNational Institute NDRI signedMOUsforcollaboration intheareas Memorandum ofUnderstanding Signed P.N. Raju,Scientist,DairyTechnologyDivision. DivisionandDr. Dairy Chemistry Senior Scientist, UG andPGlevelsweregiventoDr.RajanSharma, Appreciation lettersforexcellenceinteachingat Livestock Production&ManagementatPGlevel. UG levelandDr.A.Kumaresan,SeniorScientist, Principal Scientist,DairyTechnologyDivisionat of studentswereconferredonDr.S.K.Kanawjia, motivation ofstudentsandoveralldevelopment for contentpreparation,delivery oflectures, motivate thefacultytoadopthighstandards excellenceandrecognize andpromoteteaching and post-graduatelevelsatNDRI,Karnalto Best TeacherAwardsatunder-graduateteaching “Best Teacher Award” for Excellence in Teaching Microbiology Division. “Best DivisionalAward”waspresentedtoDairy 2014ontherecommendationofjury, February, in education/research and consultancy on 1 2013 during made achievements significant and The Heads of Divisions presented theinnovations Achievements andInnovationsinTeaching Best Divisional Award forAcademic Awards in their respective groups as given below gave theirrecommendationsfortheBestThesis The awardcommittees evaluated thethesesand presented their theses before the Award Committee. ineachdiscipline.Thestudents committees by their respectivegroupsasgivenbelow: recommendations for theBestThesisAwardsin committees evaluatedthethesesandgavetheir theses beforetheAward Committee. Theaward in eachdiscipline.Thestudentspresentedtheir programme wererecommendedbycommittees ` Dr. D.Malakar Dr. S.K.Jha Dr. S.K.Kanawjia Name oftheGuide 5000wereawarded. Two theses ineach Dr. S.K.Singla Dr. B.S.Meena Dr. BimleshMann Name oftheGuide Animal Biotechnology Agriculture Extension Dairy Technology Discipline Animal Biotechnology Agriculture Extension Dairy Chemistry Discipline st

5 • • • • • •

Award Ceremonyon23 at 21 conferred National Dairy Research Institute NDRI was Swaminathan. of GreenRevolutioninIndia-Prof.M.S. the ChiefMinister,PunjabandArchitect Processing Industriesinthepresenceof &Food forAgriculture Minister ofState Pradesh, Sh.B.L.JoshiandTariqAnwar, jointly byH.E.Hon’bleGovernorofUttar presented toDr.A.K.Srivastava,Director at TajPalace,NewDelhi.Theawardwas function Award-2013 recentlyinaglittering was conferredAgricultureLeadership (NDRI) Institute Research Dairy National on 16 participated inICARAwardCeremony’2012 (Extension)Dr. SanchitaGarai,Scientist Malik, Head,DairyMicrobiology. Mitigation” undertheguidanceofDr.R.K. Spoilage BacteriaandStrategiesforits Resistance amongDairyPathogens& Science 2012”. Sheworkedon“Bacteriocin Thesis ResearchinAgricultureandAllied Nehru Award forPG Outstanding Doctoral Dr. GurpreetKaurwasawarded“Jawaharlal as theBestProjectunderComponent4. Cattle andBuffaloes”,whichwasadjudged Embryonic and Spermatogonial Stem Cells in “Characterization and Differentiation of ICAR fortheirworkinNAIPprojectentitled DG, and Dr.M.K.Singhwerefelicitatedby Manik, Dr.S.K.Singla,DheerSingh Dr. M.S. Chauhan, Dr.P. Palta,Dr.R.S. across India. leadership amongst Educational Institutes and uponNDRIrecognizingtalent bestowed Taj Lands End, Mumbai. The award was for the year2013-14 at 12 with Dairy TechnologyDivision, wasawarded Dr. S.K.Kanawajia,PrincipalScientist, Bengal”. Members ofNewAlluvialZonesWest and GroupDynamicsofSelfHelp on “InformationCommunicationBehaviour Thesis Research in Social Science. She worked Nehru Award”forOutstandingDoctoral on 14 Honours /awards ” (Undergraduate) “Best Teacher Award” (Undergraduate) th th st February,2014 July,2013andreceived Dewang MehtaBusinessSchool Dewang Education Leadership Award th rd Convocation held October,2013at “Jawaharlal

• • • • • • • • year 2013-14at12 Teacher Award”(Postgraduate)forthe Reproduction wasawardedwith“Best Dr. A. Kumaresan, Senior Scientist, Animal Sidhu Award Division was awarded Dr. BimleshMann,Head,DairyChemistry held atNIFTEM,Kundli,Sonepat. being held at Anand, Gujarat, from 6 Foods, HealthStatusand SocialWell- Sonipat from18 Mysore, DFRL,MysoreandNIFTEM, Food Conventionorganizedby CSIR-CFTRI, Dr. M.S. Tantia, Dr. Archana Verma, Dr. A. Dr. M.S.Tantia,Archana Verma, Dr. Dibyendu Chakraborty, Dr. Avtar Singh, December, 2013. Management from15 Functional Foods inHealthandDisease Conference onBioactive Compounds and Peptide from Milk”during theNational “Characterization ofNovelOsteogenic Nutra SeniorAward”forthepaperentitled, Animal Biochemistry Division won“Bio Dr. SumanKapila,PrincipalScientist, February, 2014. of 52 Award” forbiennium2011-12ontheoccasion BasuMemorial Division received“Sukumar Head,AnimalBiochemistry Y.S.Rajput, Dr. February, 2014. year 2013-14at12 as ateacheratNDRIforthe Contributions Appreciation” inrecognitionofOutstanding of Narender Raju,Scientistgot“Certificate P.Dr. and Sr.Scientist Sharma, Rajan Dr February, 2014. 6 of Indigenous Fermented Milk Products” at Biothickening Lactic Cultures for Preparation Award”on“NovelOral Presentation Dr. PradipBeharereceived“Best Mysore. held atBioresources andBiotechnology” “International ConferenceonBiodiversity, Biotechnology atAABS/SABSponsored Award” in the year 2014 by Society for Applied Bangalore wasawarded“BestZoologist Dr. K. P. Ramesha Principal Scientist,SRS, Research Institute, NewDelhi on 21 th InternationalConferenceonFermented nd ConvocationofIndianAgricultural (2012)” in the7 th - 21 th th Convocationheldon14 Convocationheldon14 st th -16 December, 2013.

“Prof Jiwan Singh th November, 2013 November,2013 th International th - 7 th th th st

85 ndri annual report 2013-14 86 ndri annual report 2013-14 • –– Honours/Awards –– • • • during 12 Nanoemulsions Encapsulating Curcumin” and BiologicalCharacteristicsofMicro/ work “Evaluation of Physico-chemical on “The21 Convention andInternationalSymposium ICFIN –2013)23 Technology: Impact on Nutrition and Health. at InternationalConferenceonFood Chakka basedFruitDesserts”presented paper entitled“Process Standardisation for Presentation in Oral Presentation”forthe K. Jayaraj Raowas awarded Aravindakshan, Dr.C.N. Pagote Mr. D.Joshna,A.Padmaja,P. Disease inSahiwalCattle” SNP Markers Associated with Cystic Ovarian Award” for researchpaper awarded “Second K. Chakravarty Award” forthepaperentitled“Perception Division received S. V.Singh,PrincipalScientist,DairyCattle Scientist, DairyExtensionDivision andDr. Sr. Scientist,Dr.K.S.Kadian, Principal Meena, Sr.Scientist,Mrs.RituChakravarty, Dr. R.SenthilKumar,Scientist, University heldon14 Nutritional Sciences(IFANS),NewDelhi. by InternationalInstituteofFoodand Nehru University, NewDelhi,organised JNU NewConventionCentre,Jawaharlal in the “BestM.TechThesisAward” TP got Ms. Sari Anand during28 Husbandry, AnandAgriculturalUniversity, by CollegeofVeterinaryScience&Animal under EraofClimateChange”conducted Productivity and Profitability Enhancement “New DimensionalApproachesforLivestock Seminaron National ofthe Management” on “Health,Breeding& Reproduction 29/01/2014 duringfourthtechnicalSession Commercial SemenProductionSystem”on “ presentation ofaresearchpaperentitled Best OralPresentation-IPrizefortheoral Dr. MukundA.Kataktalwarereceivedthe & TechnologyofJammu,R.S.Pura,Jammu. 2014 atSher-e-Kashmir Agricultural Sciences Developing Countries”fromFebruary,14-16, Practice, Education & Research in India and Bull Housing Management Practices under Bull Housing Management Practices under

processing group th Convocation of the NDRI Deemed ConvocationoftheNDRIDeemed st Road Map forVeterinary th Mr. and Mr. D. Kumar rd “Best PaperPresentation -30 –24 Best OralPresentation th th January,2014. February, 2014. th December,2013. in the32 “Identification of on dissertation “Second Best nd Dr. B.S. and

Annual Annual were Dr.

• • • • • bubalis Antioxidant Status in Buffalo Calves (Bubalus of ChromiumonImmuneResponseand for Deka Mastitis” inthe2 Employed byPMN,during Staphylococcal NET areWeaponsofPathogenKilling Delayed Neutrophil Cargo Removal and for thepaperentitled K. Dangwereawarded“BestPaperAward” I. Bhatt, Ms. M. Kaur, S. Toki, and Dr. A. Mr. D.K.Swain,M.S.Kushwaha, Mr. 20 fortheyears2009and2010. Indian JournalofDairyandBiosciences Vol. Packaging forFoodPreservation”byin for thearticle entitled “Antimicrobial of DairyProcessing Paper” intheCategory and Mr.D.H.Patelwereawarded“Best Mr. K.N.Papinwar, Dr.B.SurendraNath collage, BakshikaTalab,Lucknow,UP26 held atChandraBhanuGuptAgriculturePG in HolisticDevelopmentofRuralLivelihood, Social DimensionsofExtensionEducation Award” intheNationalSeminar2013on Dr. H. R. Meenagot“Best Paper Presentation 2013 atUAS,Bangalore,India. Perspective, heldfrom5 Agricultural Development - A Global Educational StrategiesforSustainable International ConferenceonExtension and CropLivestock Farming System” during for thepaperentitled Singh wereawarded“BestPaperAward” Chakravarty, Dr.K. S. Kadian Mr. S.R.Kumar,Dr.B.Meena, 2013 atSKUAST,Jammu. -27 Human Welfare held on 19 Animal Welfare(IAVNAW)Conferenceon Indian AcademyofVeterinaryNutritionand Dr. V. Mani, Dr. A. K. Dang, Mr. M.Kumar,Dr.H.Kaur,A. Tyagi, December, 2013inUAS,Bangalore. Conference whichwasheldon5 Change in Haryana” at International onClimate ofDairyFarmers and Adaptation and Production held at College of Veterinary and Production held atCollegeofVeterinary Approaches to Challenges in Animal Health and NationalSeminaronBiotechnological Veterinary ScienceandBiotechnology th the paperentitled April,2013 were awarded“BestPaperAward” ) duringSummerSeason”inthe2 nd MeetingofSociety “Delayed Apoptosis, “Ameliorative Effect “Climate Variability th th -8 - 21 th and and Dr. S. V. st December, September, September, Dr. R.S. th –8 nd th th

• • • • • ((Bubalus bubalis)” during Summer in 2 ((Bubalus Antioxidant StatusinBuffaloesCalves of ChromiumonImmuneResponseand the paperentitled“AmeliorativeEffect were awarded“BestPaperAward”for Dr. A. K DangMr.RijusmitaSarmaDeka Dr. AmrishKumarTyagi,Veena Mani, Kaur, Harjit Dr. Kumar, Mr. Muneendra Dairy FoodsforManaging Cardiovascular Prize)” forthepaperentitled“Probiotic been awardedthe“BestPaperAward(First have Sunita Grover V. K.BatishandDr. Anupama, Ms.AnjuA.Achuthan, Dr. Ms. Aparna Sudhakaran V; Ms. M. at Jammu. Nagpur, Maharashtra during 8 Symposiumheld atduring theNational ISSAR by Award” received “BestPaper Dr. A.ManimaranandKumaresan S. S. Layek, Dr. S. Ravi, Dr.T. K. Mohanty, Patbanda, Dr.R.Pathak,P.Boro, Ms. S. Kumari, Dr.Shiv Prasad, Dr. T. K. Mathura on6 Science andAnimalHusbandry,DUVASU, at Guwahati15 Sustainable FoodSecurity”ISBDtobeheld in National Symposium on “Buffalo for Buffaloes”. OralPresentation Periparturient Activity and Blood Antioxidant Status in Proliferation, NeutrophilPhagocytic Chromium Supplementation onLymphocyte Award” S. Kunduwereawarded“Best Paper Kewalramani, Dr.ChanderDattand K. Tyagi, Dr.HarjitKaur, Dr.Neelam Mani, Dr.MunnendraKumar,A. Mr. RijusmitaSarmaDeka,Dr.Veena January, 2014. Animal Welfare,(19 Indian AcademyofVeterinaryNutritionand Second NationalConference,organisedby Emission: An in vitro Proprophylactic Option to MitigateMethane Award” Dr. M.S.Maheshwereawarded“BestPaper Mr. S. P. Sawant, Dr. Madhu Mohini Agricultural Science&Technology,Jammu 19-21,2013 atSher-eKashmirUniversityof Production &HumanWelfareheldSept –Health Interactions for Optimum Livestock National IAVNAWConferenceon“Nutrition for thepaperentitled for thepaperentitled“Effectof th -7 th th -16 March,2014. th –21 th Study” presented in March,2013. st September,2013) “ Nitrate as th –10 and nd th

• • • • • • 12 from 12 by IndianDairyAssociationinChennai at XILDairyIndustryConferenceorganized Kaurreceived“BestPaperAward” Gurpreet Subrota Hati, Dr. R.K. Malik and Ms. Dr. ShilpaVij,Mr.ShitalBorkar, calendar year2012. Area” in the Indian Dairyman for the Diseases” published“Technical in Aspects organised by IDA during 12 during 41stDairyIndustryandConference Paneer PressingMechanism” Nut Half-turn “Batch CapacityOptimizationforKinematic of Dairy Science for the research paper Processing Area) published in Indian Journal awarded “BestPaperAward”(Dairy Malhotra were R. P. S.MinzandDr. Mr. Mr. Kumaresh Halder, Prof. Bikram Kumar, Science, . IndianJDairy Pathogens Disease Causing Antibiotics ResistancePatternofCommon paper publishedinIndianJ.DairyScience. published inIndianJournal of DairyScience Best PaperAward”(DairyProcessingArea) and Mr.P.S.Minzwereawarded “Second Mr. MaheshKumawat,Prof. BikramKumar during 12 Dairy IndustryConference held at Chennai by at 45 Direct Investment:Prospects&Challenges” the paper“Indian Dairy Sector and Foreign awarded Mr. Shiv Raj Singh Indian Dairy Association, New Delhiat45 turn NutPaneerPressingMechanism” for KinematicHalf- Optimization Capacity “Batch Paper Award”onthepaperentitled and Dr.R.Malhotra were awarded “Best Mr. K. Halder, Mr. B. Kumar, Mr. P. S. Minz, Chennai. by IDAduring12 41 AnOverview”during Sector: Manufacturing research paper“IndianDairyEquipment published inIndianDairymanforthe Paper Award”(CommercialAspetsArea) wereawarded“Best Sirohi Smita Dr. Mr. P.S.Minz,Dr.A.K.Dodejaand 2013 atChennai. st th Dairy Industry and Conference organised Dairy Industry and Conference organised -14 th 65:47-51. nd th December,2013atChennai. Dairy Industry Conference, held in Dairy Industry Conference,heldin -14 th “Best Theme Paper Award” -14 th th Dec. 2013fortheresearch December,2013. th and -14 th were Dr. K. K. Datta were December,2013at th -14 –– Honours/Awards –– th December, December, on on by by th

87 ndri annual report 2013-14 88 ndri annual report 2013-14 • –– Honours/Awards –– • • • • on 6 Upliftment ofRuralMasses”heldatNBAGR for Sustainable Management of Livestock for on “HarmonizingPhenomics and Genomics Symposium Murrah Buffaloes”.In:National Evaluation ofFertilityinHighProducing Presentation Award”entitled“Genetic Dr. A. Mahajan Dr. C.S.Patil,VijayKumarand Mr. V. Jamuna, Dr.A. K. Chakravarty, at Anand, Gujarat during 28 ISAPM during the National Symposium held S. Kerkettareceived“BestPosterAward”by Dr. L.Sreelaand R. Pathak,Dr.P.Boro, Dr. A.Manimaran,T.K.Patbanda, Dr. Kumaresan, Dr.T.K.Mohanty,S.Ravi, Ms. S.Kumari,Dr.ShivPrasad,A. bioinformatics” organized byDWR,Karnal. Symposium on “Emerging Trends in Agri- National (SLLP)’ at Lysozyme LikeProtein N-acetyl Glucosamine (NAG) with Sperm Poster Award”onpaper“Dockingof and Mr. S.Saini,Kalra,Dr.A.K.Mohanty organised by IDA during 12 during 41stDairyIndustryandConference Khoa usingResponseSurfaceMethodology” Process ParametersforManufactureof for theresearchpaper“Optimizationof 2014. -2013) National Institute of Food Technology -2013) National Institute of Food Technology Health and Disease Management (BFHDM Compounds andFunctional Foodsin at NationalConference on “Bioactive Lactobacilli SpeciesonHT-29 CellLines” Properties ofRiboflavin Producing Poster PresentationAward”on“Adhesion Ms. KiranThakurwasawarded“Best Hebbal, Bangaloreon 16 Biologicals andVeterinaryCollege,KVAFSU, Institute ofAnimalHealthandVeterinary Quality ServicetoFarmers”organizedat ReformsandGovernanceforRegularity Areas inVeterinaryResearch,Education, & and XX Annual ConventionofIAAVR 2013 atChennai. during 13 the AdvancementofVeterinaryResearch Poster Prize” bytheIndian Association for Dr. S. Jeyakumar, was awarded

International Conference th Dr.J.K.Kaushikwereawarded“Best -7 th February,2014. th Indian VeterinaryCongress received “Best Poster th -17 th th -14 th - 30 April,2013. th on “Thrust December, December, th January, “Best Dr. • • • • • 16 from 26 New DelhiinDairyCattleNutritionDivision Science andTechnology, Govt.ofIndia, Researchers awarded byDepatment of Fellowship” forAfricanInternational Doctoral Research under CV Raman Hawassa, Ethiopiacompletedhis“Post Dr. TegeneNegesse,HawassaUniversity, NDRI, Karnal. of AnimalProductionandmanagement’at January, 2013organizedby‘Indian Society Commercial FarmingandBeyond”28-30 Livestock Production : From Traditional to Annual Convention on “New Paradigms in Grown Fodders”In:NationalSeminar&XX of Organicallyvis-à-visConventionally the paperentitled“ChemicalComposition were awarded and Late Dr.P.K.Roy,Ex-PrincipalScientist Hebbal, Bangalore. April, 2013 Dairy Science College, KVAFSU, during Storage”presentedat41 Freezing ontheTexturalAttributesofPaneer Paper” for the poster paper entitled “Effect of Dr. C.N.Pagotewasawarded“BestPoster Mr. H.Sutariya,Dr.K.JayarajRao,and Mr. H.Dwarakanath,P.Gurumurthy, November, 2013. (NIFTEM) Sonepat,Haryanaon15 Entrepreneurship and Management in the Backdrop of FSSAI Act – 2006” 26 Scenario of Dairy Food Safety and Standards presented atNationalSeminaron“Changing Parameters onQualityofSuratiPanir” Manufacturing of “Influence entitled paper the Pagote Swapna, Mr.P.Aravindakshan, Dr.C.N. Ms. Shakuntala,Mr.P.Geetha,K.S. Goregaon (E),Mumbai. Authority (FSSAI)ofIndia. Hazards” bytheFoodSafetyandStandard Biological for Panel member of“Scientific Dr. Naresh Kumar was nominated as a Intake inMurrahBuffaloHeifers”. research was “Variability inResidual Feed of CommerceandIndustry.Histopic Facilitated byFederation of Indian Chamber th March2013,BombayExhibitionCentre, “Best Poster Paper” Dr. A.Chatterjee,SeniorScientist and th Juneto24 Dr. K.JayarajRaowasawarded “Best Poster Award” th December,2013.Itwas for the poster st DIC,14 th –16 th -27 for th - th th

• • • • • • •

Food SafetyandQualityheldduring9 for DevelopingCountriesandWorkshopon on EmergingFoodSafetyRisk:Challenges Lactobacilli fromDiverseNiches”in7 and Characterization of Riboflavin Producing on the topic entitled “Isolation, Identification Scientist Award” for her oral presentation Ms. KiranThakurreceived“BestYoung Nashville, Tennessee,USA. Conference forMinorityStudents,in the 2013AnnualBiomedicalResearch outstanding contribution as reviewer for to presented was appreciation of Certificate complex, ICAR,NewDelhi Dr. NareshKumarreceived“Appreciation” and for transferoftechnologiesonL.monocytogenes student awarded“AppreciationAward” Mr. MohammadRaies-UL-Haq,Ph.D. India” during2 Key ChallengesAheadforDairyIndustryin “Food SafetyStandardsinGlobalTrade : Certificate” forpresentingatopicentitled Dr. NareshKumarreceived“Appreciation of dairyprocessing. of his outstanding research work in the field for academicsession2012-13inrecognition 20 organized byPCDF,‘Parag’Lucknowon Marketing of Milk and Milk Products, Management, QualityAssuranceand during TwodaysSeminaronDairyAnimal Regulatory ComplianceinDairyIndustry” in GlobalTradeandKeyChallengesfor entitled “CurrentFoodSafetyStandards Certificate” receivedforpresentingatopic Dr. NareshKumarreceived“Appreciation India. January, 2014 at NIFTEM, Sonepat, Haryana, S. K.Singla,Dr.M.Chauhan, Dr.T.K. Upadhyay, Dr. Y. S. Rajput, September, 2013 (ACLAB7) held inNewDelhifrom6 Asian ConferenceonLacticAcidBacteria by NAIPon16 during “Agri-businessMeet”convened Planning CommissionShriKasturinandan th in recognition ofhis inrecognition A.K.Puniya Dr. -21 Enterococci Enterococci st February,2014. Head DCP,Dr.R.S.Manik, th nd by Honorable Member of Member Honorable by -17 InternationalConference Head ABC, th July,2013atNASC . Dr. R. C. th th -11 –8 th th th th

• • • • Dr. M.Mondal,Sr.Scientistreceived “Innovative “Fellow became Dr. M.Mondal,Sr.Scientist • • •

Biological Research on 26 Climate VariabilitybyGuglyCentrefor Achieving FoodSecurityintheFaceof OccasionofNationalWorkshop,on the for PopularizationofBiologicalScience was conferredwith“GCBRAward-2013” K.Ponnusamy Dr. “Fellowship byIndianDairyAssociation”. Dr. S. K. Singla Science, India” “Fellowships ofNationalAcademyDairy Head ERS,Kalyaniwereawardedthe Head SRS,Bangalore,Dr.T.K.Dutta, Principal ScientistsNDRI,Dr.S.Kulkarni, Datta, Dr.S. K. Kanawjia, Dr. Darshan Lal, Research Associationat15 year 2013byGlobalEconomic Progress and Sadbhavna GoldMedalAward”inthe Dr. K.P.Ramesha awarded“Indira Gandhi Profession. Outstanding ContributiontoVeterinary by KarnatakaVeterinaryAssociationfor Veterinarian Award”intheyear2011-13 Dr. K.P.Rameshaawarded“Best 2014. DBT, Govt.ofIndia,4thyear. Young BiotechnologistAward (IYBA)”of January, 2014. Biodiversity Science(F.A.B.Sc.)”from of AssociationfortheAdvancement of 3years. Independent Director–Technical foraperiod Nadu MilkFederation(Aavin)”asan Dr. S.Kulkarniwasnominatedto Biotechnology”. to Fellowofthe Society forApplied Dr. K.P.Rameshaawardwas“Conferred Service totheNationheldatBangalore. Conference onIndividual Achievement and Institute, Karnalduring25 organised by National Dairy Research Mela 2014Exhibition Position” intheDairy “First bagged Division Extension Dairy at Bhubaneswar. inFebruary2014. was awarded with a , Head Dairy Extension Extension , HeadDairy th November,2013 th -27 th International –– Honours/Awards –– th February, “Tamil

89 ndri annual report 2013-14 90 ndri annual report 2013-14

6 knowledge in basic and applied fields of Dairy of fields applied and basic in knowledge information andacquiredcurrentadvanced liaison withvariousorganizationstoexchange The scientistsoftheInstitutemaintainedclose 17. 16. 15. 14. 13. 12. 11. 10. 9. 8. 7. 6. 5. 4. 3. 2. 1. No Sl. lactic acidbacteria Diversity of exopolysaccharides produced by disease prophylactic attributeagainst Alzheimer’s Development ofgeriatric dairy food having productivity indairyanimals and health of monitoring efficient for approach Bioacoustics tool: A novel non-invasive (Collaboration withGADVASU,Ludhiana) capacitation andapoptosislikechanges” buffalo bullsemen byminimizing cryo of cryopreserved Improvement infertilizability postprandial hyperglycemia probiotics lactic acid bacteriafor controlling enzymes Digestive (a-glucosidase and a-amylase) of fattyacids. of enzymesinvolvedinbiohydrogation pattern a probioticforanimalsandgenesexpression Butyrivibrio spp.itssubsequentutilizationas Diversity studyofCLAproducingindigenous training instemcelltechnologypigs. Establishment offacilitiesforresearchand farm aninals. diagnostic markerforfertilityassessmentin characterization and expressionforusingas Anti-Mullerian hormone profile, its Buffalo genomeinformationresource. consumer market. for milk fortified vitamins) & (mineral nutrient Developing &evaluationofmultiplemicro- peri-partum. in vitro competence ofneutrophilicfunctioninvivoand ofimmuno- formodulation Molecular basis responses andimmuno-seneescene. allergic Application ofprobioticsinalleviating immune genesinIndiancattleandbuffalo Genetic diversityandhaplotypingofinnate partum uterineinfectioninbuffalo. underlying theinfertilityassociated withpost- mechanisms ofovariananduterinecellsfunction Elucidating molecularandcellular productive problemsincattle,buffaloandgoat. chromosomes for detectionofgeneticalandre- associated techniquesforcharacterizationof Fluorescent various productionsystem. standardized methodology and estimates for Cost andreturnsinmilkproduction:Developing National initiativeonclimate resilient agriculture Title oftheProject RESEARCH collaborations of highproducing cows and buffaloes in situhybridization(Fish)and Pradip Behare Kaushik Khamrui S. Lathwal M. S.Chauhan A.K. Puniya A. K.Tyagi M. S.Chauhan S. De Tripura/ Avijit Haldar NER S. De Sumit Arora A. K.Dang Rajeev Kapila S. De Dheer Singh B. R.Yadav Smita Sirohi R. C.Upadhyay Co-PI Name ofPI/ organizations/ agencies. fundsfromvarious attracted research programmes of pastperformanceinconductingvarious excellence and competence scientific The Science. DST DST DBT DBT DBT DBT DBT DBT DBT DBT DBT DBT DBT DBT DBT DAHD (ICAR) CRIDA Agency Funding 2013-16 2011-14 2013-16 2012-14 2012-15 2011-14 2012-16 2012-15 2012-14 2010-13 2010-13 2011-14 2012-15 2011-14 2011-14 2012-14 2010-17 Duration 21.96 22.00 64.09 17.77 22.60 50.00 37.38 27.97 27.47 42.59 50.00 53.72 42.46 59.79 43.11 61.58 3691.25 (` inLakh) Total Cost 35. 34. 33. 32. 31. 30. 29. 28. 27. 26. 25. 24. 23. 22. 21. 20. 19. 18. embryos. goat Development potencyofparthenogenetic fertility ofcrossbredbulls. Genetic basisofinferiorspermqualityand of probablemammarybiomarkers. identification for buffalo and cattle indigenous proteome duringlactationandinvolutionin Analysis of mammary gland transcriptome and in cattleandbuffaloes. embryonic, adultand spermatogonial stem cells Characterization and differentiation of oocyte maturation andembryogenesis in buffalo. regulation processoffolliculardevelopment, Elucidating thephysiologicalandgenomic Action research. Farmers’ doorthroughmobile extension unit: Dairy Extensioneducationandservicesat and growthperformanceincrossbredcalves. of silage Evaluating cornhybridonthequality of noveldairyproducts. and goat) and their application in the formulation components fromdifferentspices(camel,sheep Characterization of colostrum Bio-active commercially availableprobioticbacteria. Status ofantibioticresistanceamong diabetes mellitus-2. against hormone,asprophylactics inducing of glucagon likepeptide-1(GLP)-1), asatiety Screening probioticsandprebioticsforexpression ICAR environmental pollutants-outreachprojectof Monitoring ofdrugresiduesandother –ICAR north easternhillregion/easternpartof India livestockfarmingin integrated people through Upliftment ofsocio-economicconditiontribal Indigenous breedprogram(Sahiwalcattle) (VTC)-Rumen Microbes. culture type programme onveterinary Network cattle ofMalnadregionKarnataka-DAH&VS ofindigenous andimprovement Evaluation treated withrecombinantbovinesamatotropin animal safetyofdairyanimalsBos Confirmation oflactationperformanceand and cow. and lutinization:acomparativestudyinbuffalo mediated generegulationduringfolliculogenesis Understanding roleofmicroRNA(mRNA) as probioticsforanimals supplementation anditssubsequentutilization reductase with differentdietarysupplementary expression patternof linoleic acid isomerase and its potentialforCLAproductionandgene Phylogenetic study of ruminal bacteria and species D. Malakar S. De A. K.Mohanty M. S.Chauhan T. K.Datta Jancy Gupta A. K.Tyagi Raman Seth S. K.Tomar Sunita Grover N. K.Goel T. K.Dutta A. K.Gupta A. K.Puniya K. P.Ramesha A. Manimaran Dheer Singh A. K.Tyagi NAIP NAIP NAIP NAIP NAIP NABARD Ltd. India Pvt. Monsanto MFPI ICMR ICMR ICAR Fund ICAR ICAR ICAR Bangalore Karnataka Govt. of Bangalore ELANCO Asia Inc. Eli Lilly DST-DAAD DST March-14 2009- 2009-2014 March-14 2008 2008- -2014 2008 -2014 2010-13 2012 -13 2012-14 2012-15 2012-15 2009-13 2012-17 2010-13 2010-13 2011-13 2012-14 2011-14 2012- 14 –– Research Collaborations –– 117.40 151.21 330.02 374.91 394.18 14.36 8.5 87.26 43.00 54.06 71.00 50.00 25.00 17.25 64.72 185.00 8.25 5.00

91 ndri annual report 2013-14 92 ndri annual report 2013-14 –– Research Collaborations –– 59. 58. 57. 56. 55. 54. 53. 52. 51. 50. 49. 48. 47. 46. 45. 44. 43. 42. 41. 40. 39. 38. 37. 36. for betterinvitrofertilizingability. Enhancing developmentcompetence ofoocytes adhesion-promoting probioticsurface proteins. Countering gastrointestinaltract pathogensby of humanlactogerrin. Development oftransgenicgoatforproduction on fertility. spermatogenic cell markers for early prediction Infertility incrossbredbulls:Searchfor by pregnancyassociatedproteins(PAPs). Early detectionofpregnancyincow and buffalo buffalo underheatandnutritionalstress. eventsinsheepand ofpregnancy recognition maternal Deciphering themechanismofaberrant quality characteristicsofbuffalomilk Investigations onhighpressureinducedeffect controlled release. Micro-encapsulation ofbacteriocinsfortheir Scheme ondairymicrobesundernetworkmode. institute herd(CIRBHisar-125001). Network project onbuffalo improvement- Unit (CIRBHisar-125001). Network projectonbuffaloImprovement-Field Agricultural Research. andKnowledgeSysteminE-Publishing in ICAR (FMS) system financial including (MIS) system information ofmanagement Implementation Prelim examinationbyASRBICAR managing anon-linesystemforNET/ARS Developing commissioningoperating& management underNARS(eGranth) and information of digitallibrary Strengthening Strengthening statisticalcomputingforNARS. detection of adulterants using chemical biology. Detection andmitigationofdairypathogens metals andbacterialcontaminationinmilk. for analysis of pesticide residues aflatoxin, heavy andmicro-techniques Development ofbiosensors enhanced healthattributesbyICAR. A valuechainoncomposite dairyfoods with potential useinfunctionaldairyfoods. nutraceuticles frommilkandIndianherbsfor Novel Approachesfortheproductionof Development ofe-coursesforB.Tech. fibrous feeds. methanogenesis andutilizationofpoorquality and wild ruminants and impact of additives on Rumen microbialdiversityindomesticated animal management. Development ofwirelesssensornetworkfor and pashminagoats. quality animal production from elite buffaloes Value chainonzonefreeclonedembryosfor T. K.Datta J. K.Kaushik M. S.Chauhan A.Kumaresan A.K. Mohanty T. K.Datta A. K.Singh R. K.Malik R. K.Malik A.K. Chakravarty Avtar Singh Meena Malik J. K.Kewalramani A. K.Srivastava B. R.Yadav R. Malhotra Y. S.Rajput N. K.Goel A. K.Singh A. K.Singh A.A. Patel S. K.Sirohi T. K.Mohanty S. K.Singla NFBSFARA NFBSFARA NFBSFARA NFBSFARA NFBSFARA NFBSFARA NFBSFARA NFBSFARA Network Network Network NAIP NAIP NAIP NAIP NAIP NAIP NAIP NAIP NAIP NAIP NAIP NAIP NAIP 2013-16 2012-15 2012-16 2012-16 2012-15 2010-15 2011-13 2011-14 2010-13 2007-17 2007-17 20 14 Upto March 2014 Upto March 2014 Upto March 2014 Upto March March-14 2010- 2009-2014 2008-14 2009-14 2009-14 2009-14 2008-14 2008-14 2009-14 107.17 123.68 253.68 241.30 200.39 77.11 66.02 52.75 95.00 00.00 60.20 15.00 31.41 32.45 38.41 55.24 158.39 2256.01 357.98 230.38 362.76 78.89 248.62 824.11 74. 73. 72. 71. 70. 69. 68. 67. 66. 65. 64. 63. 62. 61. 60. Total milk fat and its validation under field conditions. of Quality based methodologytoascertainthe Development ofchromatographicandPCR tribal inDisasterProneAreasofUttarakhand. Preparedness oflivestock rearers among the peptides. aminopeptidases fortheproductionofbioactive Recombinant expression of lactobacilli fertility. animal buffalo milklactadherinforaugmenting Cloning andmolecular characterizationof from whey. tolerant andmetabolicallyengineeredyeast Whey to Biofuel: Bioithanal Production bystress designed fortheIndianMarketconditions. Development ofresilientprobioticfoods Preparation, characterizationandapplication. Mineral boundwithbasedingredients: of functionalbeverage. peptides andtheirapplicationforthedevelopment andimmunomodulatory antioxidative with Production of buffalo casein hydrolysates enriched Probiotics. Healthy Foods:ProductionofstableandActive embryonic stemcells. Development ofparthenogeneticgoatfrom selected adulterantsandcontaminantsinMilk Development ofstripbaseddetectionTests for validation ofbovinegrowthhormoneRIAKit. milk compositionduringlactationinbovinesand Estimation of hormonal profile, metabolites and detection inbuffaloes. Luteinizing hormone based sensor for estrus methane emissionindairyanimals. Evaluating theimpact of ration balancing on selected antibioticandpesticideresidue. Imprinted polymersforsensingandremovalof Dr. S.Ayyappan,Hon'bleSecretary DAREandDirectorGeneral,ICARfelicitatedbyNDRIduring Dr. D.SundaresanMemorialLecture on11 Vivek Sharma H. R.Meena J. K.Kaushik Aasma Turan Shilpa Vij R. K.Malik Sumit Arora Rajesh Kumar Surajit Mandal M. K.Singh Rajan Sharma Mahendra Singh Dheer Singh S. Kundu Y. S.Rajput th February, 2014 SERB ICSSR DBT DBT NFBSFARA DST MOFPI MFPI MFPI NFBSFARA (MOFPI) SERB DAE NFBSFARA NDDB NFBSFARA 2013-14 2014-2016 2013-2016 2013-16 2013014 2013-15 2013-15 2013-15 2013-15 2013-17 2013-14 2013-15 2013-16 2013-14 2013-15 –– Research Collaborations –– 14.85 29.23 45.48 39.35 112.4 99.98 46.85 50.00 35.82 76.33 49.84 24.96 141.25 58.20 13028.28 28.84

93 ndri annual report 2013-14 94 ndri annual report 2013-14 7 • • • • KRISHI VIGYANKENDRA(KVK) Balance wereprocured. Machine, Refractrometer,pHMeter,Moisture ProcessingUnit of Dairy up vocationintheseareas. For thislab.,allitems preservation andalsomobilizethesegroupstotake of dairy processing and fruitand vegetable field the in skills impart and awareness create to NGOs. This lab was established with theobjective different selfhelpgroupsofNDRI,DRDAand empowerment labforcapacitybuildingof women establish wastakento A newinitiative awareness inthefieldofdairyingandhome campaigns wereorganizedinordertocreate Forty threewomenempowermenttrainingand DAIRY EXTENSIONDIVISION Total Self HelpGroup(SHGs) Awareness campaign inwomen empowerment through Awareness campaignonchildcareandnutrition Awareness campaignonbalanceddiet preservation Capacity buildingoffarmwomeninfruitsandvegetables Awareness campaignoncleanmilkproductionpractices products Capacity buildingoffarm women invalueadded milk farming Capacity Buildingoffarm-womeninscientificdairy milk products farmwomen on smallscaleproductionofvalueadded Entrepreneurship developmentandcapacitybuildingof Name oftheProgramme

MAINSTREAMING OFGENDERISSUES EMPOWERMENT OFOMENAND district YamunaNagar(Haryana). preparation of milk products at KVKDamla in women 20 to provided was Training (Haryana). in KVK Damla district Yamuna Nagar Category institchingofladiesgarment SC of women 30 to training Provided enterprises infoodprocessing. Karnal toapprisethemabout varioussmall women from Gurgaon at HTI, Uchani district 20 the to provided was training days two A various villagesofKarnaldistrict. vegetables preservationfor130womenin days durationwereorganizedinfruitsand Four off campus training programmes of 1-2

viz. EcoMilkTesting Amritpur Kalan Kulwehri, Subri Subri Kulwehri, Subri Shahpur Shahpur, Bazidpur Subri, Bazidpur, Shahpur, Bazidpur Subri, Bazidpur, Empowerment Lab. Women On Campus/Village women weretrained. in theirfamilies.Bytheseprogramme,600farm from dairyingandmaintainhealthyenvironment women so that they could generate more income science and also improve the skills of the farm • • • • •

Week on5 Feeding Milk Breast World celebrated KVK ATMA, districtKutch. from Gujarat sponsored by Project Director, women 40 for Farming Dairy Scientific on A four day special programme was organized Karnal. Director, ATMA,AjmeratKVK,NDRI women fromRajasthansponsoredbyProject ATMA district Kangraapartone for 30 Office, Choupal, Shimla and Project Director Pradesh sponsored byBlock Development farming for40womenfromstateofHimachal dairy scientific on each duration days five organized were programmes training Two processing. kitchen gardening,childcareandmilk offoodgrains, storage like various topics district Karnalfor103ruralwomenon were organizedinvarious villagesof programmes training campus off Four from Karnaldistrict. entrepreneurship development forwomen were organized forskill development and programmes training campus on Following participated. district Karnalinwhich 62ruralwomen

th August2013invillageKailash Total Courses Conducted 14 16 43 1 2 1 4 1 4 Participants No. of 182 225 600 12 27 17 59 14 64 Total Preparation oflowcostnutritiousrecepies Food processing Block printing Clean milkproduction Embroidery Preparation ofmilkbasedproducts Preservation offruits&vegetables Topic On-campus trainingprogrammeorganized for womenatNDRI Duration 3-4 2-3 2-5 1-3 14 2 5 No. ofcourse –– Empowerment of Women and Mainstreaming of Gender Issues–– 2 2 4 3 1 1 1 No. ofwomenparticipants 287 31 71 76 54 18 19 18

95 ndri annual report 2013-14 96 ndri annual report 2013-14 8 PUBLICATIONS Farming. NDRIPublicationNo.101/2013. Dairy onOrganic Bulletin Nishant (2013)ATechnical Chander, Harika,A. S., Kumar, Naresh, Kumar, Kamboj, M. L., Rai, Saroj, Prasad, Shiv, Datt, Research Institute,Karnal. Smarika-2014. D. K.andMeena,B.S.(2014)DairyMela Upadhyay, Mridula,Singh,SohanVir,Gosain, Chandra,Kundu,S.S.,Ashutosh,Datt, No. 123/2014,pp:1-360+xiv. . NDRIPublication LivestockProductivity Enhancing (2014) AdvancedBreedingandAlliedTechnologiesfor Chakravarty, A.K.,Vohra,V.andBhakat,M. 2013. NDRIPublicationNo.113/2013,pp:1-35. A. andMohanty,T.K.(2014)NDRISireDirectory Chakravarty, A.K,Gupta,K.,Bhakat,M.,Singh, 1-27. of Dairy Bull. NDRI Publication No.106/2013, pp: Evaluation Pradeep, B. ( 2013)BreedingSoundness K., Gupta,A.Kumaresan,A.,Bhakat,M.and A. K.,Kumar,Nishant,Mohanty,T. Chakravarty, Publication No.115/2013,pp:1-20. Prajannan Kritrim Gaye Evam Bhains KiNasal Sudhar Mein Kayaryarat Singh, P.,Mohanty,T.K.andA.P.(2013) Bhakat, M.,Chakravarty,A.K.,Gupta, NDRI PublicationNo.114/2013,pp:1-64. and Baithalu,R.K.(2014)InFarmTrainingManual. Gupta, A.K.,Singh,P.,Prasad,S.,Kumaresan,A., Bhakat, M., Chakravarty, A. K., Mohanty, T. K., 111/2013. Janne KiJarooratKyon?NDRIPublicationNo. Anjali, A. (2013) DudharuGaayon Ki Sharirik Dasha NDRI PublicationNo.109/2013. Samdhan . Va Kaaran Samsya: Ki Utarne Dudh Mein Anjali, A.andUpadhyay,R.C.(2013)Bhainson Rakhrakhav Va Dekhbhaal Ki Bhainson Va Gaay Hetu Prajanan Atyadhik GarmiKeMausamMeinUchitPashu Anjali, A.,Upadhyay,R.C.andKumar,P.(2013) Ki Aavakshyakta Mein DudharuGaayon Mein SukshamPoshak Tatvon Anjali, A.andUpadhyay,R.C.(2013)GrishamKaal Institute Publications . NDRIPublicationNo.108/2013. . NDRIPublicationNo.97/2013. Published byNationalDairy . NDRI Kendra Anushandhan Ltd., Kanpuron14 organized forM/s NamasteIndiaFoods Pvt. Milk and Milk Products. For Training programme of Assurance Quality for Techniques Analytical Thakur, G.andSingh,V.K.(2014)Microbial Kumar, N.,Raghu,H.V.,Arora,S.,Tehri, NDRI PublicationNo.98/2013. on SensoryEvaluationofMilkandProducts. Khamrui, K. and Khetra, Y. (2013). Practical Manual (2013) Sabikhi, L.,Thompkinson, D. K.andKhetra,Y. Publication No./2014. . NDRI Materials ofPackaging Testing Products: ofDairy ofPackaging Manual S. (2014).Practical Raju, P.N.,Singh,A.K.,Meena,G.S.andGanguly, Industry. Microbiological Quality and Safety Monitoting in Dairy Raghu, H.V.,Kumar,NareshandMalik,R.K. 341-49. Karnal, Research Institute, Dairy National Technologies for Enhancing Livestock Productivity Technologies. In:Advance BreedingandAllied Assessment of Advance Breeding &Management Nagrale, B.andDatta,K.(2014)Economic No. 119/2014. for theDairyandFoodIndustries. Processes “Alternative Manualon S. (2014).Laboratory Meena, G.S.,Singh,A.K.,Raju,P.N.,andBarod, Publication No.121/2014. Practical ManualonBy-productsTechnology.NDRI Meena, G.S.,Gupta,V.K.andHussain,S.A.(2014). NDRI PublicationNo.110/2013. Liye Bygyanik Dairy FarmingKaByabaharik Gyan. Jancy (2013)KisanoKe and Gupta, Kumar, Senthil Meena, B.S.,Singh,Omvir,Mohammad,Asif, Towards Excellence. NDRIPublicationNo.118/2014. Malik, MeenaandSabikhi,Latha(2014)AJourney Biomarker Discovery.pp:120. in Proteomicsfor Advances P. andKumarS.Recent Mohanty, A.K.,Kaushik,J.Malakar,D.,Behare, 105/2013. and DairyDevelopment.NDRIPublicationNo. Chander (2014)MilkProductionManagement Lathwal, S.S.,Kumaresan, A., Prasad, Shiv, Dutt, Microbiology Division.pp:1-94. Laboratory Manual- Cheese Technology NDRIPublicationNo.124/2014. th -19 th January,2014atDairy NDRIPublication . 2 pp: nd .

. NDRI Publication No. 122/2014. pp: 1-64. No.122/2014. pp: . NDRIPublication Research Strengthening of Library Resources for Agricultural Yadav, B.R.andSingh, P.(2014)Manualon 112/2013. pp:1-60. Problems inFarmAnimals.NDRIPublicationNo. (2013) Yadav, B.R.,Tonk,R.K.andSrivastava,A. for QualityBuffaloProduction Prasad, ShivandChauhan,M.S.CloningResearch Singla, S.K.,Manik,R.S.,Singh,M.Palta,P., 207-16. Karnal,pp: ResearchInstitute, National Dairy Resilient LivestockandProductionSystem. R.C., Sirohi,S.andSingh,A.K.(Editors), Climate Buffalo Milk Production. In:Singh,S.V.,Upadhyay, Upadhyay, R.C.(2013)Water Footprint of Cattle and Sirohi, S.,Pandey,D.,Singh,S.V.,Bansod,S.and Bhumika Hetu ShukshamPosakTatvoKe Kshemta MeSudhar Evam, Upadhyay, R.C.(2014)GaonKiPartirodhak Singh, S.V.,Kumar, Suresh, Beenam, Singh, A. K. National DairyResearchInstitute,Karnal. Sambandhit Takniko Ka Hastantaran. Publishedby Vikash Pariyojana Ke Tahat Pasupalan Ebam Swasthya Sirohi, N. S. (2014) Pusar Gaon Mein Ekikrit Grameen Singh, Mohinder, Ponnusamy, K., Jha, S. K. and 1 -7,2014.pp:-182,NDRI,Karnal. Lecture CompendiumforTrainingProgramme. May Microbiological Quality Assurance for Dairy Supply Chain. Sharma, R.andKumar,N.(2013)Chemical “Analysis ofMilk,MilkProductsandCattleFeed (2014) Sharma, VivekArora,SumitandGoyal,Ankit Microbiology Division.pp:1-182. -7, 2013 by Dairy Chemistry Division & Dairy Training programme organized during May 1 Chain. For Supply Dairy for Assurance Quality Chaudhary, P.(2013)ChemicalandMicrobiological Sharma, R.,Kumar,N.,Raghu,H.V.and and DairyMicrobiologyDivision.pp:1-170. 08 –20 programme organizedforEgyptianScientiston Products”. ForTraining Safety ofLivestock Ensuring for Concepts Quality and Tehri,N.(2013)“Current Sharma, R.,Kumar, N.,Raghu,H.V.,Lawaniya, R. pp: 62. Edition. October.NDRIPublicationNo.25/2006. Status PaperonCytogeneticandReproductive Lecture Compendium of A Lecture th . NDRIPublicationNo.120/2014. April2013byDairyChemistryDivision . pp:81. Short Training on ”. K. (2013) Genetic and non-genetic factors affecting factors affecting non-genetic and Genetic (2013) K. Chakravarty, A.K.,Gupta, K.andSachdeva,G. Kokate, L.S., Singh, A., Banu,R.,Gandhi,R.S., buffaloes. J.AdvancedVety.Res., polymerphisms ofleptingene withinIndianwater Prasad, Jagdish(2013)ExploringDNA Kale, D.S.,Yadav,B.R.,Mukherjee,Anupama, Sci., 66(3):218-23. buffaloes forlifetimeperformances.IndianJ.Dairy Gupta, A.K.(2013)GeneticevaluationofMurrah Jamuna, V.,Chakravarty,A.K.,Patil,C.S.and 231-34. in Tharparkarcattle.IndianJ.Dairy Sci., 66(30): performance onthebasisofearlyexpressedtraits oflifetimeK. andAhmed,S.(2013)Prediction Hussain, A.,Gupta,A.K.,Manoj,M.,Dash,S. : 8-15. Sci., 1(1) Vety. J. lactation curvemodels.Indian curve formilkyieldinSahiwalcattlebydifferent V. (2013)Detectionofdifferentshapeslactation H., Sonawane,G.S.,Ahlawat,A.R.,andRaja,T. P. Dongre, V.B.,Gandhi,R.S.,Singh,A.,Vataliya, Indian J.Anim.Res.,47(4):321-26. Study ofvariousperformance traitsinRathiCattle. Avtar, Dangi, P. S, Singh, Rajbir, Pundir, R. K., Singh, with Mastitis incidence. J. Anim. Res., 3(1) : 103-08. Lactoferrin genePolymorphism and its association Soumya, N. P and Vohra, V. (2013) Identification of Chopra, Alka,Gupta,I.D.,Verma, Archana, No. KF668651 region, exon1andpartialcds.NCBIAccession (2013) Bosindicuslactoferrin(LF)gene,promoter Chopra, A.,Gupta,I.D.,Verma,A.andVohra,V. Murrah Buffalo.BuffaloBulletin, and its Association withIncidence of Mastitis in in Caspase Activating Recruitment Domain 15 Sonawane, Gokul S. (2013) Genetic Polymorphism I. D.and Chauhan, Indrasen,Verma,A.,Gupta, 5 :1-6. Networks. production indairycowsusingArtiifcialNeural Sharam, A.K.(2013)Lifetimemilkamount Chaturvedi, S.,Yadav,R.L.,Gupta,A.K.and Research Papers DAIRY CATTLEBREEDING Chaudhary, VijayandVerma,N.K.(2013) International J. Recent Res.andReview, International J.Recent 3:20-26. 32(2):723-28. –– Publications ––

97 ndri annual report 2013-14 98 ndri annual report 2013-14 and Raina, V. S. (2013) Influence of microclimate of Influence (2013) S. V. Raina, and Singh, A.P.,R., A.K.,Gupta,K. (2013): 11-18. accoustic sensing. discrimination of individual dairy cattlethrough and Chandra,G.(2013)Effectiveaccurate Chakravarty, A. K., Sharma, P. C., Sharma, V. V., Gupta,A.K.,Mohanty,T.Ruhil,P., Singh, Y.,Lathwal,S.S.,Rajput,N.,Raja,T. Sci., 67(1):1-11. connectionist models. J.Indian Society of Agril. Economic traitsinMurrahbuffaloeswith Malhotra, R.andRuhil,A.P.(2013)Predicting Sharma, A.K.,Jain,D.Chakravarty, crossbred bulls. IndianJ.Anim.Sci.,83(7):732-35. in aspiration needle fine of process the by altered (2013) Testicular biometry and semen quality is not S, Chakravarty,A. K.andVenkatasubramanian, V. Muhammad Aslam, M. K.,Mohanty, T. K.,Prasad, Rajak, S.K.,Kumaresan,A., Gaurav,M. K., J. Anim.Res.,48(1):6-10. gene promoterregioninBosindicuscattle. (2014) Sequencecharacterizationoflactoferrin Raja, K. N., Gupta. I. D. and Verma, Archana 81 (3):474–80. behavioral traits in crossbred cattle. Theriogenology, seminal andsexual and itsassociationwith gene P. N.(2014)Polymorphismofgrowthhormone Pal, Aruna,Chakravarty,A.K.andChatterjee, 10.4061 /2011/507346. International, CD14 GeneofCrossbred Cattle. MolecularBiology Molecular Characterization and SNP Detectionof Chatterjee, P.N.andChakravarty,A.K.(2013) Pal, Aruna,Sharma, A,Bhattacharya, T. K., (5) :402-06. traits inkaranfries cattle. IndianJ.Anim.Res..,47 trends in milk yield and Milk production efficiency K. (2013).Phenotypic,geneticandenvironmental Chakravarty A.K.,Gupta,K.andSachdeva,G. Nehra, Manju,Singh,Avtar,Gandhi,R.S., cattle. IndianJ.Anim.Res.,48(1):11-13. enzyme anditsassociationwithmastitisinSahiwal CD14 GenePolymorphism using HinfIrestriction Kumar, S.RajeshandChaudhari,M.V.(2013) I. D.,Verma,Archana, Kumar, Virendra,Gupta, Indian J.Anim.Sci.,83(4):385-89. monthly testdaymilkyieldinKaranFriescattle. –– Publications –– Article ID507346,13Pages,doi: , 146 Behaviour Sci.,146 Applied Anim. Indian

Chpater 16.pp:179-186. : (13)978-81-8321-293-9,ISBN: (10)81-8321-293-X. Complex-B, DurgaNursery Road, Udaipur.ISBN Agrotech Publishing Academy, 11A-Vinayak Lathwal, S.S.).Publishedby Mrs. Geeta Somani, management (Ed.Ruhil,A.P.; Mohanty,T.K.and In Dataminingtechniquesforfarmanimal Gupta, A. K., Manoj, M., and Kumar, A. (2013) Book Chapters/TechnicalArticles of Sahiwal Cattle.Indian J.Anim. Res.,47(2):172-74. RFLP withAluIRestrictionEnzymeinTLR4Gene Kumar, DineshandRajeshKumarS.(2013)PCR- Wakchaure, R.S.,Gupta,I.D.,Verma,Archana, traits ofdairybulls.Asian Pacific J. Reprod. 3(1):doi: exotic geneticinheritanceonsemenproduction A. and Prasad, S.(2014)Effect ofpedigree and T. K.,Gupta,A. K.,Chakravarty,A. K.,Manimaran, S.S.,Kumaresan,A.,Mohanty, Vijetha, B.T.,Layek, J. Anim.Sci.,84(2):79-82. used asatooltopredictejaculatequality?Indian crossbred bulls:Cantesticularmeasurementsbe Prasad, S.(2014)Breedingsoundnessevaluationin K., Muhammad Aslam, M. K.,Manimaran, A.and A. A., Mohanty,T.K.,Chakravarty,A.Gupta, Vijetha, B.T.,Rajak,S.K.,Layek,S.;Kumaresan, buffalo. BuffaloBulletin, traits and incidence of mastitis in Indian Murrah immune functiongenesassociatedwithproduction inproductionand variability S. (2013)Genetic Verma, Archana,Gupta,I.D.andGandhi,R. cattle. IndianJ.Anim.Res.,48(2):99-102. genotypes onmilkproductiontraitsinSahiwal Yadav, B. R. (2014) Effect of beta lactoglobulin Tolenkhomba, T.C.,Mayengbam,Pravaand Reprod., 3(1):1-5. J. Pacific production traitsofdairybulls.Asian pedigree andexoticgeneticinheritanceonsemen Manimaran, A.andPrasad,S.(2014)Effectsof A. K., A. K.,Chakravarty, Mohanty, T.K.,Gupta, Thippeswamy, V. B., Layek, S.S.,Kumaresan, A., partial cds.NCBIAccessionNoKC679298. A. (2013).BosindicusCARD15gene,exon12and N., Rajeshkumar,S.,Chauhan,I.andChopra, Soumya, N. P.,Gupta,I.D.,Verma, A., Raja, K. 431-34. humid period in India. characteristics of Murrahbuffalobullduringhot modification onsexualbehaviourandsemen 32(2):729. Indian J.Animal Sci., 83(4): Karnal. pp:42-49. Upadhyaya R C, Sirohi S and Singh, A K.), NDRI, Livestock andProductionSystem(Editors: Singh SV, greater tolerance toheatstress. In:Climate Resilient R. S. (2013) Selection of livestock breeds for Singh, Avtar,Sivalingam,JayakumarandGandhi, Publication No.123/2014,Karnal.pp:57-73. Chakravarty, AK,Vohra,VandBhakatM.)NDRI for EnhancingLivestockProductivity. (Editors: In: AdvancedBreedingandAlliedTechnologies lactation milkyieldusingtestdaymodels. Singh, AvtarandGian(2014)Predictionof pp :7-12. 6-72013. Resources,KarnalonFeb Animal Genetic Bureauof andNational Animal Biodiversity organized bySocietyforConservationofDomestic of LivestockforUpliftmentRuralMasses and Genomics forSustainable Management National SymposiumHarmonizingPhenomics under current scenario. InCompendium ofXI oflivestockdiversity inIndiaConservation Gandhi, R.S.andSingh,Avtar(2014) K. andPrasad,Shiv(2014)Post-thawed andfresh Galmessa, U.,Mohanty, T. K., Raina, V. S. Gupta, A. Ethiopia. J.Agril.Res.andDevelop., 3:82-93. the greaterAddis milk shed, central highlandsof of natural pasture hayfor feeding dairy cattle in status ofproduction,conservationandutilization Kitaw, Getu and Bediye. Seyoum (2013) The Feyissa, Fekede,Prasad,Shiv,Assefa,Getinet, Prod, 13:121-35. central highlandsofEthiopia.EthiopianJAnim composition ofnaturalpasturehayatHoletta, storage systemanddurationonmineral and Prasad,Shiv(2013)Effectsofharvestingstage, Feyissa, Fekede,Bediye,Seyoum,Assefa,Getinet Prod., (DOI10.1007/s11250-013-0477-1). semi-arid tropicalenvironment.Trop.Anim.Hlth. immune functionofcrossbred dairycowsunder Effect of micronutrient supplementation on the De, K., Pal,S., Prasad, S. and Dang, A. K. (2013) Buffalo Bulletin, Optimum Semen Production in Buffalo Bulls. and OtherManagementPracticesfor Nutritional Dahiya, Satbir Singh and Singh, Pawan (2013) Research Papers MANAGEMENT LIVESTOCK PRODUCTION 32(1):277-84. J. Sci.&SustainableDevelop., 1:1-12. intake, digestibilityand milk yield of dairy cows. concentrate mixture and their effect on nutrient and Rai,S.N.(2013)Inclusion ofpulsehullsin Mekete, Berhane, Prasad, Shiv, Assafa, Getnet Sci., 3(2):76-80. dairy farmers.Res. of commoninterestgroup(CIG)inempowering Uzmakalam, Bhakat,M.andSingh,A.(2012)Role Lal, Banwari,Chauhan,J.P.S.,Sankhala,Gopal, 12 (1-2):52-56 Toxicology, and Pharmacology Vety. dairy farm.J. ofclinicalmastitiscasesinorganizedtreatment Estimation of antimicrobial drug usage for Sreela. L,Kumar,KausalandPrasad,Shiv(2013) Manimaran, A.,Layek,S.S.,Kumaresan, cattle. Vety.Res.Forum4(2):85–89. fetus andsubsequentfertilityinfourcrossbred combination therapy on expulsion of mummified Behera, K.(2013)Effectofestradiol+cloprostenol Mohanty, T.K.,Prasad,Shiv,Layek,S.and Suresh,S.,Kumaresan, A.,Chand,Subhash, Knowledge, 13(2):404-08. Kran Fries Crossbred cows. Indian J. Traditional on milkproductionandimmunomodulationin (2014) Effect of Satavari (Asparagus recemosus) Kumar, Santosh,Mehla,R.KandSingh,Mahender Anim. Res.,47(10):439-42. performance of lactating crossbred cows. Effect offence-linefeedingonthebehaviourand Kumar, ChandanandKamboj,M.L.(2013) The IndianJ.Anim.Sci.,83(10):991-97. calves andtheirdamsindairycows–areview. ofweaning ontheperformanceandbehaviour Kamboj, M.L.andKumar,Ajesh(2013)Effectof Vety. Medicine,2(1):64-67 puberty of Sahiwal heifers, Int. J. Agril. Sci. and (Asparagus recemosus) on thebodyweightand Chouhan, N.(2014)EffectofthefedSatavari Jamara, M.S.,Mehla,R.K.,Singh,M.,Ali,M.M., Ethiopia. J.Agril.andSustainability2(1):1-21. in WesternOromiaMilkValueChain,Oromia, (2013) Dairyproduction potential and challenges Alganesh, Prasad, Shiv and Kebede, Late Mulugeta Galmessa, Ulfina,Dessalegn,Jiregna,Tola, Int. J.LivestockProd.,5(4):65-70. under computer assisted semen analyzer (CASA). spermatozoa motioncharacteristicofSahiwalbulls J. Anim.HusbandryandDairy –– Publications –– Indian J.

99 ndri annual report 2013-14 –– Publications ––

Mondal, Goutam, Sarkar, T. K., Khan, H. M., discrimination of individual dairy cattle through Medhi, Dinamani and Bhakat, M. (2013) Copper acoustic sensing. Applied Anim. Behavior Sci., 146: and zinc supplementation to corriedale lambs in an 11-18. organized farm of Kashmir valley: A preliminary study. Indian J. Anim. Nutr., 30(2): 145-48. Singh, M., Raina, V. S., Mohanty, T.K., Gupta, A. K., Tripathi, M. K. and Rao, T. K. S. (2013) Effect Pankaj, P.K., Raina, V. S., Pourouchottamane, of some selected amino acids on preservation of R., Venkatsubramanian, V., Mohanty, T. K. cattle bull semen in soybean based extender. Vety. and Muzamil, S. (2014) Relative efficiency of Practitioner, 14 (2): 261-62. sterilization methods for the treatment of glassware part of artificial vagina to be used for bovine semen Sreenath, D. D., Mehla, R. K., Md. Aslam, M. K., collection. Applied Biological Res., 16 (1):1-4. Prasad, Shiv and Manoj, M., (2013) Factors affecting first lactation rate in Karan Fries crossbred cows, Pankaj, P. K., Raina, V.S., Pourouchottamane, BIOINFOLET 10(4C): 1581-84. R., Venkatsubramanian, V., Mohanty, T. K. and Muzamil, S. (2013). Effect of deworming on Syridion, D., Layek, S. S., Mohanty, T.K., seminal characteristics of Karan Fries bulls. Applied Kumaresan, A., De, K., Manimaran, A., Prasad, Biological Res., 15 (2): 1-6. Shiv and Venkatasubramanian, V. (2013) Effect of production systems on milk quality parameters in Patbandha, T. K., Mohanty, T. K., Layek, S. S., Holstein friesian crossbred cows. Indian J. Dairy Kumaresan, A., Malhotra, R. , Kantwa, Suresh C., Sci., 66(5): 424-31. Ruhil, A. P. and Prasad, Shiv (2013). ROC analysis of pre-partum feeding time can accurately predict Vijetha, B.T, Rajak, S. K., Layek, S. S., Kumaresan, post-partum metritis development in Holstein A., Mohanty, T. K., Chakravarty, A. K., Gupta, A. Friesian (HF) crossbred cows. J. Vety. Behavior: K., Muhammad Aslam M.K, Manimaran, A. and Clinical Applications and Res. J. Vety. Behavior, 8 : Prasad, Shiv (2014) Breeding soundness evaluation 362-66. in crossbred bulls: Can testicular measurements be used as a tool to predict ejaculate quality? Indian J. Rajak, S. K., Kumaresan, A., Gaurav, M. K. Aslam, Anim. Sci., 84 (2): 79-82. M K Muhammad, Mohanty, T. K. Prasad, Shiv, Chakravarty, A. K. and Venkatasubramanian, V. Vijetha, B. T., Layek, S. S., Kumaresan, A., (2013) Testicular biometry and semen quality is not Mohanty, T. K., Gupta, A. K., Chakravarty, A. altered by the process of fine needle aspiration in K., Manimaran, A., Prasad, Shiv (2014) Effects of crossbred bulls. Indian J. Anim. Sci., 83 (7): 732–35. pedigree and exotic genetic inheritance on semen production traits of dairy bulls. Asian Pacific J. Ruhil, A. P., Mohanty, T. K., Rao, S.V.N., Lathwal, Reprod. 3(1): 13-17. S. S., Subramanian, V.Venkata (2013). Radio- Frequency Identification: A cost effective tool to Technical/Popular Articles improve livestock sector. The Indian J. Anim. Sci., 83(9) : 871-79. Kumar, Chandan and Kamboj, M.L. (2013). Occupational zoonoses: Human at risk. Livestock Sharma, Amit, Prasad Shiv, Singh, Y. and Line, 7(2): 21-24. Bishisth, R. (2013) Effect of polyherbal preparation supplementation on immunity and udder health of Kumar Chandan, Kamboj, M.L. and Kumar Ajesh periparturient Karan-Fries crossbred dairy cows. J. (2013). Livestock disaster management. Livestock Applied Anim. Res.. Published online http://dx.doi. Technology, 3(7): 34-35 org/10.1080/ 09712119. 2013.842477). Patbandha, T. K., Kumaresan, A., Mohanty T. K., Singh, Pawan, Kumar, Dharamendra, Kumar, Layek, S.S and Pathak, Rupal (2013) Understanding Pradeep, Singh, Inderjeet and Yadav, P.S. (2013) pig behavior for better management. The Pig J. Cryopreservation and quality assessment of 116-23. buffalo bull semen collected from farmer’s doorstep. Agril. Res., 2(2): 148-52. Patbandha, T. K., Bhakat, M., Mohanty, T. K., Kumaresan, A. and Layek, S. S. (2013) Behavioural

ndri annual report 2013-14 report ndri annual Singh, Yajuvendera, Lathwal, S. S., Rajput, understanding of transition cow for better herd Nitender, Raja, T. V., Gupta, A. K., Mohanty, health and animal welfare. Indian Dairyman, 65(7): 100 T. K., Ruhil A. P. (2013) Effective and accurate 64-67 –– Publications ––

Prakash, M. Arul, Kumaresan, A. and Manimaran, buffaloes under in vitro conditions. Indian J. Anim. A. (2014). Factors affecting reproductive efficiency Sci., 83: 1203-06. in dairy cows. Kalnadai Kathir (Dec.-Jan.) pp: 70. Datt C, Malik, S. and Kundu, S. S. (2013) Effect Prakash, M. Arul, Kumaresan, A., Manimaran, A. of substitution of maize with wild sotti (Curcuma and Boopathi, V. (2013) Importance of goat rearing. zeodaria) rhizomes in the diets of crossbred pigs on Kalnadai Kathir (Oct.-Nov.) pp: 68.-69 feed intake, growth and feed conversion ratio. Ind. Vet. J., 90: 37-38. Books Datt C., Malik, S., Haldar, Avijit, Singh, N. P., Datta, Bhakat, M., Mohanty, T. K., Prasad, Shiv, Mohanty, M. and Kundu, S. S. (2013) Effect of probiotics A. K., Bahera, Sudarshan, P. (2013) Practical Manual: supplementation on egg quality and production Management of Dairy Animals for class XII: CBSE. performance in Gramapriya birds under warm and humid climate. Indian J. Anim. Nutr., 30: 320-24. Bhakat, M., Mohanty, T. K., Prasad, Shiv, Mohanty, A. K., Sudarshan, Patbandha, T. K., Behera, K. Ishtiyak A. Mir, Sehgal, J. P. and Sirohi, S. K. (2013) (2013) Students Hand Book: Management of Dairy Isolation and hydrolytic enzymes production Animals (Theory) for class XII: CBSE. potential of fungal isolation from murrah buffaloes (Bubalus bubalis). Indian J. Anim. Nutr., 30 (2):162-68. Kumar, Chandan and Kamboj, M. L. (2013) Fence-line Feeding System for Dairy Cows. Lambert Jain P, Mohini, Madhu, Tyagi, A. K. and Singhal, Academic Publishing, Germany (pages 108). K. K. (2013) In vitro evaluation of feed on supplementation of herbal mixture at different Kumar, N., Mohammad, A., Bahera, P., Bhakat, levels. Indian J. Anim. Nutr., 30 (2) : 132-40. M. (2013) Practical Manual: Milk Production: CBSE. Jha, N., Sachinandan, De, Kundu, S. S. and Datt, Singh, Sohanvir, Kumaresan, A., Dutt, Chander, Chander (2013) Identification of methanotrophs in Mandal, Goutam, Manimaran, A. (2013) Animal the buffalo rumen by 16S rRNA sequence analysis. Nutrition and Reproduction Hand Book (for class XI) Indian J. Anim. Sci., 83: 1323-27. Theory. Published by CBSE, New Delhi Kumar, S., Choudhury, P. K. et al. (2013) New Training Manual aspects and strategies for methane mitigation from ruminants. Appiled Microbiology and Biotechnology, Lathwal, S.S., Singh, P., Bhakat, M., Kumar, R. and 1-14. Dutt, Chandra (2014) Training Manual on Baigyanic Pashupalan Evm Vikas for ATMA, Chittorgarh. pp: Kumar B and Sirohi, S. K. (2013) Effect of isolates of 64. fiber degrading bacteria on body weight changes, milk production and its composition, nutrient DAIRY CATTLE NUTRITION utilization in lactating murrah buffaloes. African J. Biotech., 12(12), pp: 3302-08. Research Papers Kumar, B, Sirohi, S. K., Puniya, A. K., Sheel, Ahmed H. A., Sirohi, S. K., Puniya, A. K. and Sheel, Rakesh and Ahmed, H. A. (2013) Morphological, Rakesh (2013) Influence of addition of propionic biochemical and molecular characterization of fiber acid producing bacteria on dry matter digestibility degrading bacterial isolation from buffalos rumen. and rumen fermentation profiles in wheat straw Indian J. Anim. Nutr., 30(3): 246-51. containing total mixed ration in vitro. Indian J. Anim. Nutr., 30 (3) : 272-80. Kumar, B., Sirohi, S. K. (2013) Effect of isolate of rumen fibrolytic bacterial culture supplementation Chand P, Sirohi, Smita and Sirohi, S. K. (2013) on fibrolytic strain in lactating murrah buffaloes. Production and demand estimates of livestock feed dol: 10.5455/vetworld, 14-17. and fodder in Rajsathan. Indian J. Anim. Nutr., 30 (2): 149-56. Kumar, M., Kaur, Harjit, Tyagi, Amrish, Mani, Veena, Deka, Rijusmita Sarma, Chandra, Gulab

Datt C, Kumar, Ajay and Kundu, S. S. (2013) Effect and Sharma, Vijay Kumar (2013) Assessment of 2013-14 report ndri annual of different levels of added selenium without or chromium content of feedstuffs, their estimated with arsenic on rumen fermentation parameters in requirement, and effects of dietary chromium 101 –– Publications ––

supplementation on nutrient utilization, growth Prusty, S., Kundu, S. S., Kumar, A., Ruhil, A. P. performance, and mineral balance in summer- and Datt, C. (2013) Quantitative prediction of exposed buffalo calves (Bubalus bubalis). Biological indigestible cell wall fraction of tropical livestock Trace Element Res., ISSN 0163-4984 DOI 10.1007/ forages. Indian J. Anim. Sci., 83 (12): 1347-50. s12011-013 Prusty, S., Kundu, S. S., Kumar, Ajay, Ruhil, A. P. Kumar, M., Kaur, Harjit, Tyagi, Amrish, and Datt, Chander (2013) Quantitative prediction Kewalramani, Neelam J., Mani, Veena, Deka, of indigestible cell wall fraction of tropical forages Rijusmita Sarma, Sharma, Vijay Kumar, Chandra, used in livestock feeding. Indian J. Anim. Sci., Gulab and Dang, Ajay Kumar (2013) Effect 83:1347-50. of feeding inorganic chromium on growth performance, endocrine variables, and energy Prusty, S., Mohini, Madhu, Kundu, S. S., Kumar, metabolites in winter-exposed buffalo calves Ajay and Datt, Chander. (2013) Methane emissions (Bubalus bubalis ). Biological Trace Element Res., from river buffaloes fed on green fodders in ISSN 0163-498 DOI 10.1007/s12011-013-9808-3 relation to the nutrient intake and digestibility. Vol.155-oct 2013. Trop. Anim. Health and Production. DOI 10.1007/ s11250-013-0447-7. Kumar, M., Kaur, Harjit, Phondba B. T., Deka, Sheel, R., Sirohi, S. K., Kumar, Brishketu and Rijusmita Sarma, Chandra, Gulab, Mani, Veena and Ahmed, H. A. (2013) Effect of mixture of medium Gupta, Neelam ( 2013) Effect of zinc treatments on chain fatty acids and unsaturated fatty rich oils on lead exposed periparturient bovine lymphocytes methane production and rumen fermentation in in vitro on their proliferation and superoxide vitro. Indian J. Anim. Nutr., 30(3): 256-61. dismutase (SOD) expression Indian J. Anim. Sci. 83 (12) : 1261–66. Sirohi, S. K. and Goel, Navneet (2013) Effect of various levels of bromochlormethane and Kumar, B. and Sirohi, S. K. (2013) “Effect of fiber level on in vitro methane production and cellulose degrading bacteria isolation from murrah fermentation pattern. Indian J. Anim. Sci., 83(8): buffaloes on in vitro fiber digestibility. Indian J. 798- 801. Anim. Nutr., 30(2): 128-31. Sirohi, S. K. and Goel, N. (2013) Effects of various Malik, S., Kumar, S., Sarkar, B., Doley, S., Datt, levels of bromochlomethane and fibber level on Chander and Datta, M. (2013) Evaluation of in vitro methane production and fermentation Tripura Brown, a native poultry germplasm at pattern. Indian J. Anim. Sci., 83 (8). the organized farm and field conditions. Ind. Vet. J. 90: 26-29. Sirohi, S. K., Choudhry, Prashant Kumar, Puniya, Anil Kumar, Singh, Dheer, Dagar, Sumit Singh Mondal, G, Sarkar, T. K., Khan, H. M., Medhi, and Singh, Nasib (2013) Ribosomal IT IS sequence- D. and Bhakat, M. (2013). Copper and Zinc based diversity ananlysis of anaerobic rumen supplementation to lambs: A preliminary study. fungi in cattle fed on high fiber diet. Annals of Indian J. Anim. Nutr., 30:145-48. Microbiology DOR 10.1007/s 13213-013-0620-2.

Mondal, G. and Kakati, B. K. (2013) Effect of Sirohi, S. K., Chudhry, Prem Prashant, Singh, supplementation of concentrate on performance Nasib, Singh, Dheer and Puniya, Anil Kumar of crossbred lambs in Kargil. Indian J. Anim. Nutr., (2013) The 16S rRNA and mcrA gene based 30:12-16. comparative diversity of methanogens in cattle fed on high fiber based diet. Gene, 523:161-66. Mondal, G, Kakati, B. K., Das, T. K. and Mughal, A. H. (2013) Evaluation of local feeds and performance Sirohi, S. K., Dagar, Sumit Singh Singh, Nasib, of sheep under field condition of Kargil. Indian J. Chaudhary, Prem Prashant, Punia, Anil Kumar Anim. Nutr., 30 : 99-102. and Singh, Dheer (2013) Differential rumen microbial dynamics and fermentation parameters Prusty, S., Mohini M., Kundu S. S., Kumar, A. in cattle fed on high fiber and high concentrate and Datt, C. (2013) Methane emissions from river diets. Indian J. Anim. Nutr., 30(1):60-66.

ndri annual report 2013-14 report ndri annual buffaloes fed on green fodders in relation to the nutriet intake and digestibility. Tropical Anim. Sirohi, S. K., Walli, T. K., Garg, M. R. and Kumar, 102 Health and Prod.. DOI 10.1007/s11250-013-0447-7. Brishketu (2013) Effect of formaldehyde treated –– Publications ––

mustard cake on nutrient utilization and milk Puniya, A. K., Kumar, Sanjay, Sirohi, S. K and production performance in crossred cows fed Singh, Kishan (2013) Livestock Methane Emission wheat straw based diet. Indian J. Anim. Nutr., 30(1). and Global Warming: Risks and Remedies. In book “Human and Animal Health” Sudhi Ranjan Garg Book Chapters (Ed.) published by Satish Serial Publishing House, Delhi-110033,ISBN 978-93-81226-30-8 pp:431-44. Chaudhary, P.P., Sirohi, Sunil Kumar, Ahmed, Haidar Ali (2013) Diversity and geographical Puniya, A. K, Kumar, Sanjay, Sirohi, Sunil Kumar distribution of rumen methanogens. In: Sirohi SK, and Singh, Kishan (2013) Livestock Methane Walli TK, Singh B, Singh N (Eds), Livestock Greenhouse Emission and Global Warming : Risks and Gases: Emission and Options for Mitigation. Satish Serial Remedies. In book “Human and Animal Health” Publishing House, Delhi, India. pp : 83-102. Sudhi Ranjan Garg (Ed.) published by Satish Serial Publishing House, Delhi-110033,ISBN 978-93- Datt, C., Kumar, Ajay, Puniya, Monika, Kundu, S.S. 81226-30-8 pp:431-444 and Das, T. K. (2013) Climate change and its impact on livestock production performance: In: Climatic Sirohi, S. K, Choudhury, Prasanta, Puniya, A. K. Variability: Impacts on Agriculture and Allied Sectors and Chaudhary, Prem Prashant (2013). Harnessing (Edited by: M. Datta, Dhiman Daschaudhuri and The Diversity of Rumen Microbes. In book S.V. Ngchan) New India Publishing Agency, New “Microbial Diversity and its Application” Barbudde, Delhi. Chapter 32, pages 254-272. S.B., R. Ramesh & N. P. Singh (Eds.) published by New India Publishing Agency : ISBN -9381450668. Goel, N., Sirohi, Sunil Kumar (2013) Saponins as a promising agent for rumen methane mitigation. Sirohi, S. K., Choudhury, Prasanta, Puniya, A. K. In: Sirohi SK, Walli TK, Singh B, Singh N (Eds), and Chaudhary, Prem Prashant (2013) Harnessing Livestock Greenhouse Gases: Emission and Options for The Diversity of Rumen Microbes. In book Mitigation. Satish Serial Publishing House, Delhi, “Microbial Diversity and its Application” Barbudde, India. pp 213-234. S.B., R. Ramesh & N.P.Singh (Eds.) published by New India Publishing Agency : ISBN -9381450668. Kumar, P. Choudhury, Sirohi, Sunil Kumar, Puniya, Anil Kumar, Chaudhary, Prem Prashant Sirohi, S. K., Walli, T. K., Singh, Bhupinder, Singh, (2013) Harnessing the diversity of rumen microbes Nasib (2013) Livestock Greenhouse Gases: Emission using molecular approaches. In: Sirohi SK, Walli and Options for Mitigation. Satish Serial Publishing TK, Singh B, Singh N (Eds), Livestock Greenhouse House, Delhi, India (ISBN 978-93-81226-52-0). Gases: Emission and Options for Mitigation. Satish Serial Publishing House, Delhi, India. pp 65-82. Sirohi, S. K, Pandey, Poonam, Mohini, Madhu (2013) Contribution of Ruminants towards global Kundu S.S., Das, T. K. Dutt C., Hooda O. K, warming in India vis a vis the world. In: Sirohi Ashutosh and Upadhyay R. C. (2014) Climate SK, Walli TK, Singh B, Singh N (Eds), Livestock change impacts management of livestock. In: Greenhouse Gases: Emission and Options for Climate Change and Crop Production (Edited by Mitigation. Satish Serial Publishing House, Delhi, Dagar J.C, Arunachalan A, Singh A.K) A publishers India. pp 15-26. and Distributors, Jaipur 302 003 Rajasthan. Sirohi, S.K, Mohini, Madhu, Puniya, Anil Kumar Pandey, P., Goel, Navneet, Sirohi, Sunil Kumar (2013) Mitigation options for enteric methane (2013) Plant bioactives as rumen fermentation emissions from dairy animals. In: Sirohi SK, modulators. In: Sirohi SK, Walli TK, Singh B, Walli TK, Singh B, Singh N (Eds), Livestock Singh N (Eds), Livestock Greenhouse Gases: Emission Greenhouse Gases: Emission and Options for and Options for Mitigation. Satish Serial Publishing Mitigation. Satish Serial Publishing House, Delhi, House, Delhi, India. pp 257-284. India. pp 125-142.

Puniya, A.K., Dagar, Sumit Singh, Kumar, Sanjay, Singh, N. and Sirohi, Sunil Kumar (2013) Sirohi, Sunil Kumar (2013) Biological approaches Biochemistry, bioenergetics and genetics of for reducing methane generation in ruminant, methanogenesis in rumen methanogenic archaea. In: Sirohi SK, Walli TK, Singh B, Singh N (Eds), In : Sirohi SK, Walli TK, Singh B, Singh N (Eds),

Livestock Greenhouse Gases: Emission and Options for Livestock Greenhouse Gases: Emission and Options for 2013-14 report ndri annual Mitigation. Satish Serial Publishing House, Delhi, Mitigation. Satish Serial Publishing House, Delhi, India. pp 293-300. India. pp 103-120. 103 –– Publications ––

Books production in peripartum Sahiwal cows. Asian Austr. J. Anim. Sci., 26 (11): 1569-76. Datt, Chander Singh, Sohanvir, Kumaresan, A., Mondal, Gautam and Manimaran, A. (2013) Chandra, G., Aggarwal, A. and Upadhyay, R. C. Animal Nutrition and Reproduction (Dairy (2013) Liver enzymatic profile of periparturient husbandry)-Practical Manual for Class-XI. CBSE, Sahiwal cows as affected by vitamin E Siksha Kendra 2, Community Centre, Preet Vihar, supplementation. Indian J. Dairy Sci., 66 (1) : 51-54. Delhi-110092, India. pp: 76. Chaudhari B. K., Singh, M., Maurya, P. K., Singh A. Kundu, S. S, Mani, Veena, Paul, S. S. and Dahia, K., Singh, J. K. (2012) Stress markers in the plasma S.S. (2013) Nutrient requirements of Animals- Cattle and milk of Murrah buffaloes during summer. and Buffaloes, published by ICAR Agricultural Reviews, 34 (1) 21- 35.

DAIRY CATTLE PHYSIOLOGY Choudhary B. K., Singh, J. K. Singh, M., Maurya, P. K. and Singh, A.K. (.2012). Management of Research Papers reproductive performance in buffalo during summer season. Wayamba J. Anim. Sci., ISSN: 2012- Aggarwal, A. and Chandra, G. (2013) Antioxidant 578 (w.w.w. wayamba Jourrnal. com). status, hormone levels and immunity in crossbred cows of different levels of production. Indian J. Dang, A. K., Mukherjee, J., Chaudhary, M., Prasad, Anim. Res., 47 (6) : 492 - 97. S., Mohanty, A. K., Kapila, S. and Kapila, R. (2014) In vitro phagocytic activity of blood and milk Behera, P., Kumar, R., Sandeep, I.V.R., Kapila, neutrophils against Saccharomyces cerevisiae R., Dang, A. K. and Kapila, S. (2013) Casein hydrolysates enhance osteoblast proliferation and in primiparous and multiparous Karan Fries differentiation in mouse bone marrow culture. Food crossbred cows throughout the dry period and Biosci., 2 : 24-30. lactation cycle. Indian J. Anim. Sci., 84 (3): 262–66. March 2014. Bhabesh, Mili, B., Pandita, S., Bharath Kumar, B.S., Singh, A. K., Mohini, Madhu and Manju Ashutosh Dang, A. K., Mukherjee, J., Jamwal, M., Singh, S., (2013) Xanthine oxidase activity during transition Mohanty, A. K., Prasad, S., Kapila S. and Kapila, period and its association with occurrence of R. (2013) Isolation of exfoliated somatic cells from postpartum infections in Murrah buffalo (Bubalus buffalo milk. Buffalo Bull., 32(1) : 53-58. bubalis) African J. Biotech., 12 (32) : 5101- 04. De, K., Pal, S., Prasad, S. and Dang, A. K. (2013) Bhan, C., Singh, S.V., Hooda, O. K., Upadhyay, R. Effect of micronutrient supplementation on the C. and Beenam, B. (2013) Influence of temperature immune function of crossbred dairy cows under variability on physiological, hematological and semi-arid tropical environment. Trop. Anim. Hlth. biochemical profile of growing and adult Karan Prod., (DOI 10.1007/s11250-013-0477-1). Fries cattle. Indian J. Anim. Sci., 83 (10) : 1090 – 96. Dipak, B., Upadhyay, R. C., Chaudhary, U. Chandra, Bhan, Singh, S. V., Upadhyay, R.C., B., Kumar, R., Singh, S.V., Ashutosh, Jagan Hooda, O.K. and Beenam, B. (2013) Impact Mohanarao G., Shamik P., Mukherjee, A., Das, T. of temperature variability on physiological, K., De, S. (2013) Seasonal variation in expression hematological and biochemical profile of growing pattern of genes under HSP70. Cell Stress and and adult Murrah buffaloes. J. Agrometeoro. 15 Chaperones. DOI 10.1007/s 12192-013-0469-0. (Special Issue-II) : 146-52 . Ganaie, A.H., Hooda, O.K., Singh, S.V., Ashutosh Chandra, G. Aggarwal, A. and Upadhyay, R.C. and Upadhyay, R.C. (2013) Effect of vitamin C (2013) Effect of vitamin E and zinc supplementation supplementation on immune status and oxidative on erythrocyte antioxidant enzymes and plasma stress in pregnant Murrah buffaloes during total antioxidant activity in Sahiwal cows. Indian thermal stress. Indian J. Anim. Sci. 83 (6): 649 – 55. J. Dairy Sci. 66 (5) : 412 - 17. Ganaie, A. H., Hooda, O. K., Singh, S.V., Ashutosh

ndri annual report 2013-14 report ndri annual Chandra, G. Aggarwal, A. and Upadhyay, R. C. and Upadhyay, R.C. (2012) Responses of vitamin (2013) Effect of vitamin E and zinc supplementation C supplementation on biochemical, hormonal 104 on energy metabolites, lipid peroxidation and milk and physiological parameters of pregnant Murrah –– Publications ––

buffaloes during hot-humid conditions. Indian J. Mukherjee, J., Kaswan, S., Chaudhary, M., Anim. Nutr., 29 (3): 214 - 21. Mohanty, A. K. and Dang, A. K. (2013) Blood and milk cell counts and phagocytic activity of Jamara, M.S., Mehla, R.K. Singh,M., Ali, M.M. neutrophils isolated from crossbred cows during Ali and Nirmala Chouhan.(2014) Effect of the fed different seasons. Indian Vet. J., 90: 48-51. Shatavari (Asparagus racemosus) on body weight and puberty of Sahiwal heifers. Int. J. Agric.Sci. & Mukherjee, J., Varshney, N., Chaudhury, M., Vet. Med. 2(1) : 64 - 67. Mohanty, A. K. and Dang, A. K. (2013) Immune response of the mammary gland during different Janjanam, J., Jamwal, M., Singh, S., Kumar, S., stages of lactation cycle in high versus low yielding Panigrahi, A. K., Hariprasad, G., Jena, M. K., Karan Fries crossbred cows. Livestock Sci., 154 : Anand, V., Kumar, S., Kaushik, J. K., Dang, A. 215-23. K., Mukesh, M., Mishra, B. P., Srinivasan, A., Reddy, V. S. and Mohanty, A. K. (2013) Proteome Nazir, A. Mir, Kumar, Parveen, and Ovais, Aarif analysis of functionally differentiated bovine (Bos (2013) Effect of Tinospora cordifolia supplementation indicus) mammary epithelial cells isolated from on certain biochemical parameters in lactating milk. Proteomics, 13: 3189–3204.doi: 10.1002/pmic. Int. J. 201300031. Murrah buffaloes during winter season. Scientific Res., 2 (7): 515 - 16. Kapila, R., Sebastian, R., Phani Varma D.V., Sharma, R., Kapasiya, M., Salingati, V., Kapila, Nazir, A. Mir, Kumar, Parveen, Wani, Sajad A., S and Dang, A. K. (2013) Comparative activation Shergojry, Shahid A., Ashutosh, and Aarif, Ovais of innate immunity on prolonged feeding of (2014) Milk production status of Murrah buffaloes Lactobacilli fermented milks. Microbiology and on Tinospora cordifolia supplemented diet with Immunology, 57 (11) : 778-84. special reference to immunological, metabolic and hormonal profile. Anim. Sci. Reporter, 8 (1): 18-25. Kumar, M., Kaur, H., Tyagi, A. K., Kewalramani., N. J., Mani, V., Deka, R. S. Sharma, V. K., Chandra, Pampori, Z. A. and Pandita, S. (2013) Analysis of G. and Dang, A. K. (2013) Effect of feeding age and sex bias in inos gene expression pre and inorganic chromium on growth performance, post endotoxin challenge in buffalo lymphocytes. endocrine variables, and energy metabolites in Vet. Pract., 14(1) : 130-32. winter-exposed buffalo calves (Bubalus bubalis). Biol. Trace Elem. Res., 155 (3) : 352-60. Pampori, Z. A. and Pandita, S. (2013) Gender and age bias in immune competence and oxidative Kumar, V. and Kumar, P. (2013) Impact of thermal stress markers in Murrah buffaloes (Bubalus bubalis) stress on rectal, skin surface temperatures, Indian J. Anim. Sci., 83 (7): 717–724. respiration rate, heat load index and heat storage in lactating murrah buffaloes (Bubalus bubalis). Buffalo Pampori, Z. A. and Pandita, S. (2014) Age and sex Bulletin, 32 (2) : 1141-44. related variability in physiological and immune responses to endotoxin challenge in Murrah Maurya, P. and Aggarwal, A. (2013) Cellular buffaloes (Bubalus bubalis) J. Appl. Anim. Res., adaptation and antioxidant activities in crossbred *DOI:* 10.1080/09712119.2014. 883318 Feb 2014 cows during transition period. Indian Vet. J., 90 published online. (8): 11-14. Pathan, M. M., Kaur, H., Mohanty, A. K., Kapila, S. Maurya, P. and Aggarwal, A. (2013) Effect of alpha and Dang, A. K. (2014). Comparative evaluation tocopherol acetate and zinc supplementation on immunity and reproductive health of Karan Fries of neutrophil competence and activity of cows and cows during dry period and early lactation. Vet. buffaloes around peripartum. TAAR: J. Applied Pract., 14 (1): 153 - 56. Anim. Res. (Manuscript ID: 896260, dx.doi.org/10 .1080/09712119.2014.896260). Michelle, C. Lallawmkimi, Singh, S. V., Upadhyay, R. C. and De, S. (2013) Impact of vitamin E Shanmugam M, Pandita, S. and Palta, P. (2013). supplementation on Heat Shock Protein 72 and Influence of gonadotropins on steroidogenesis by antioxidant enzymes in different stages of Murrah large follicle buffalo (Bubalus bubalis) granulosa 2013-14 report ndri annual buffaloes during seasonal stress. Indian J. Anim. cells under in vitro serum-free conditions” Indian Sci., 83 (9): 909 – 15. J. Anim. Sci., 83(12):1267-70. 105 –– Publications ––

Singh, A. K, Pandita, S., Upadhyay, R.C., Chandra, Singh, S.V., Kumaresan, A., Datt, Chander., Mondal, C., Chaudhari, B. K., Maurya, P. K. (2013) Effect Goutam and Maran, Mani (2013) Animal Nutrition of pre-partum supplementation of vitamin E and Reproduction (Dairy Husbandry), published by Murrah buffaloes on metabolic adaptation and Central Board of Secondary Education, pp: 138. growth performance of calves. Indian J. Anim. Res., 47(3):196-204. Datt, Chander., Singh, S.V., Kumaresan, A., Mondal, Goutam and Maran, Mani (2013) Animal Singh, M., (2013) Manipulating the Postpartum Nutrition and Reproduction (Dairy Husbandry) - Physiological and Metabolic Adaptation to Practical manual, published by Central Board of Secondary Education, pp: 76. Augment Milk Production in Bovines. Proc. XXIIth Annual conference of Society of Animal Physiologist of India (SAPI) on a theme Physiology ANIMAL BIOTECHNOLOGY and Nutri-genomics, held at DUVASU, Mathura from Nov., 19-21, pp: 117-85. Research Papers Augustine, R., Malik, H. N., Singhal, D. K., Mukherjee, Singh, M., and Prakash, B. S. (2013) Oxytocin A., Malakar, D., Thomas, S., and Kalarikkal, release response in hand and machine milking N. (2013). Electrospun polycaprolactone/ZnO Murrah buffaloes. Indian J. Anim. Sci., 83(63):615-17. nanocomposite membranes as biomaterials with antibacterial and cell adhesion properties. J. Singh, M., Choudhary, B. K., Singh, J. K., Singh, Polymer Res., DOI 10.1007/s10965-013-0347-6. A. K., Maurya, P. K. (.2013) Effect of thermal load on buffalo reproductive performance in buffalo Balhara, A. K., Gupta, M., Singh, S., Mohanty, A. during summer season. J. Biological Sci.,1 (1) : 1-8. K. and Singh, I. (2013). Early Pregnancy Diagnosis in Bovines: Current Status and Future Directions. Singh, S. P., Mehla, R. K. and Singh, M. (2012) The Scientific World .J Volume: Article ID 958540,10 Plasma hormones, metabolites, milk, production pp http://dx.doi.org/10.1155/2013/958540. and cholesterol levels in Murrah buffalos fed with Asparagus racemosus in transition and postpartum Bhardwaj, A., Nayan, V., De, S., and Goswami, period. Trop. Anim. Health Prod., 44:1827-32. S. L. (2013). Differential expression profiling of recombinant bovine inhibin-alpha at reduced Vyankanti, P. A., and Anand Laxmi (2014) Effect of temperature. Indian J. Anim. Res., 47 (1) : 61 - 65. thermal stress on functional properties of caprine De, A. K., Garg, S., Singhal, D. K., Malik, H. N., hepatocytes culture in vitro. J. Adv .Vet. Res., 4 : 1-5. Mukherjee, A., Jena, M. K., Kumar, S., Kaushik, J. K., Mohanty, A. K., Das, B. C., Bag, S., Bhanja, Popular Articles S. K. and Malakar, D. (2013). Derivation of goat Sehgal, J. P., Singh M. and Roy, A. K. (2013). embryonic stem cell-like cell lines from in vitro produced parthenogenetic blastocysts. Small “Feeding strategies for improving milk productivity Ruminant Res., 113 : 145 - 53. from cattle in urban and peri-urban areas” Livestock and Feed-trends; 2(4): 9-24. De, A. K. and Malakar, D. (2013). In vitro differentiation of developmentally competent Singh, A.K., Kushwaha, R., Vaidya, M.M., oocytes by monitoring Glucose 6 Phosphate Singh S.V. and Upadhyay R.C. (2013). Shelter Dehydrogenase (G6PDH) activity with brilliant Management: Sustainable Improvement of Animal cresyl blue stain in Jamunapari goat. Anim. Sci. Production and Health. Livestock Line, 7 (5): 13-17. Reporter, 7 (2) : 60 - 66.

Singh, A.K., Singh, S.V., Upadhyay, R.C. and Gadave, K. S., Panda, S., Singh, S., Kalra, S., Sharma, V.K. (2013). Environmental and Nutritional Malakar, D., Mohanty, A. K., Kaushik, J.K. (2013). Factors causing Mastitis. Livestock Line, 7 (4): 12. Structural and functional insights into the catalytic inactivity of the major fraction of buffalo milk Books xanthine oxidoreductase. PLOS ONE 9(1): e87618. doi:10.1371/journal.pone.0087618.

ndri annual report 2013-14 report ndri annual Singh, S.V., Upadhyay, R.C., Sirohi, S. and Singh A.K. (Eds) (2013) Climate resilient livestock and Janjanam, J., Jamwal, M., Singh, S., Kumar, S., 106 production system. ISBN: 978-93-5156-150-7, pp: 286. Panigrahi, A. K., Hariprasad, G., Jena, M. K., –– Publications ––

Anand, V., Kumar, S., Kaushik, J. K., Dang, A. K., glutathione level and embryonic gene expression. Mukesh, M., Mishra, B.P., Srinivasan, A., Reddy, J. Assisted Reproduction and Genetics, DOI 10.1007/ V. S. and Mohanty, A. K. (2013). Proteome analysis s10815-013-0116-9. of functionally differentiated bovine (Bos indicus) mammary epithelial cells isolated from milk. Mukherjee, A., Dass, G., Rao, J.M.G., Gohain, M., Proteomics, 13 : 3189 - 3204. Brahma, B., Datta, T. K. and De, S. (2013). Absolute copy number differences of Y chromosomal genes Kumar, P., Rajput, S., Verma, A., De, S., Datta, T. K. between crossbred (Bos taurus x Bos indicus) and (2013). Expression pattern of glucose metabolism Indicine bulls. J. Anim. Sci. and Biotech., 4 (1) : 15. genes in relation to development rate of buffalo (Bubalus bubalis) oocytes and in vitro-produced Palai, T. K., Bisoi, P. C., Maity, A., Behera, P.C., embryos. Theriogenology. 80 (8): 914 - 22. Sahoo, G., Polley, S. and S. De. (2013). Prolificacy in Raighar Goats is Independent of Fec B Gene. Kaushik, R., Singh, K. P., Kumari, A., Singh, M. K., Veterinary World 6 (8) : 479 - 81. Manik, R. S., Palta, P., Singla, S. K. and Chauhan, M.S. (2013). Isolation, characterization, and Patel, A.V., Singh, K. P., Varshney, N., Chauhan, M. EGFP expression in the buffalo (Bubalus bubalis) S., Palta, P., Singla, S. K. and Manik, R. S. (2013). mammary gland epithelial cell line. In Vitro Cellular Expression pattern of transcription factors during & Developmental Biology – Animal 49 (1) : 1 - 7. zygotic genome activation in buffalo (Bubalus bubalis) embryos produced in vitro. Indian J. Biotech., Kumar, R., George, A., Sharma, R., Singla, S. 12: 323 - 29. K., Chauhan, M. S., Manik, R. S. and Palta, P. (2013). Production of blastocysts from aggregates Patra, M.C., Rath, S. N., Pradhan, S. K., Maharana, of buffalo embryonic stem cells and putative J. and De, S. (2014). Molecular dynamics simulation tetraploid buffalo embryos. Indian J. Anim. Sci., 83 of human serum paraoxonase 1 in DPPC bilayer (10) : 1033 - 38. reveals a critical role of transmembrane helix H1 for HDL association. Biophysics of Structure and Kumar, S., Singh, M. K., Kumar, V., Palta, P., Singla, Mechanism 43: 35-51. S. K., Manik, R. S. and Chauhan, M. S. (2013). Characterization of the coding region of basic Priya, D., Selokar, N. L., Raja, A. K., Saini, M., fibroblast growth factor (FGF2) c-DNA (open Sahare, A. A., Nala, N., Palta, P., Chauhan, M. S., reading frame) in buffalo cumulus cells. Indian J. Manik, R. S. and Singla, S. K. (2014). Production of Anim. Sci., 83 (6): 72–00, July 2013/Article. wild buffalo (Bubalus arnee) embryos by interspecies Malik, H. N., Singhal, D. K., Mukherjee, A., Bara, somatic cell nuclear transfer using domestic buffalo N., Kumar, S., Saugandhika, S., Mohanty, A. K., (Bubalus bubalis) oocytes. Reproduction in Domestic Kaushik, J. K., Bag, S., Das, B. C., Bhanja, S. K. and Animals. doi: 10.1111/rda.12284. Malakar, D. (2013). A Single Blastomere Sexing of Caprine Embryos by Simultaneous Amplification Selokar, N. L., Saini, M., Palta, P., Chauhan, M. of Sex Chromosome Specific Sequence of SRY and S., Manik, R. S., Singla, S. K. (2014). Hope for Amelogenin Genes. Livestock Sci. 157: 351 - 57. Restoration of Dead Valuable Bulls through Cloning Using Donor Somatic Cells Isolated from Malik, H. N., Singhal, D. K., Saugandhika, S., Cryopreserved Semen. PLOS ONE 9(3): e90755. Dubey, A., Mukherjee, A., Singhal, R., Kumar, S., Mohanty, A. K., Kaushik, J. K., Bag, S., Das, B.C., Sharma R, Kamble N, George A, Chauhan MS, Bhanja, S. K. and Malakar, D. (2013). Generation of Singla S, Manik RS and Palta P. (2013). Effect of parthenogenetic goat blastocysts: Effect of different TGF-β1 Superfamily Members on Survival of activation methods and culture media. Zygote Buffalo (Bubalus bubalis) Embryonic Stem-like Cells. doi:10.1017/S0967199413000580. Reproduction in Domestic Animals: Jan 16. doi: 10.1111/rda.12126. Mukherjee, A., Malik, H.N., Saha, A.P., Dubey, A., Singhal, D. K., Boateng, S., Saugandhika, Singh, M. K., Saw, S., Singh, K. P., Saini, N., S., Kumar, S., De, S., Guha, S. K. and Malakar, Kaushik, R., Manik, R. S., Palta, P., Singla, S. K. D. (2013). Resveratrol treatment during goat and Chauhan M. S. (2014). Expression of apoptosis oocytes maturation enhances developmental related genes in buffalo embryos produced 2013-14 report ndri annual competence of parthenogenetic and hand-made through in vitro fertilization and parthenogenetic cloned blastocysts by modulating intracellular activation. Indian J. Anim. Sci., 84 (2): 146–151. 107 –– Publications ––

Singhal, D. K., Singhal, R., Malik, H. N., Kumar, Haq, M.R.U., Kapila, R. and Saliganti,V. (2014) S., Kumar, S., Mohanty, A. K., Kaushik, J. K., Consumption of β-casomorphins-7/5 induce Malakar, D. (2014). Molecular cloning, sequence inflammatory immune response in mice gut characterization and recombinant expression of through Th2 pathway. J. functional foods, 8 : 150–60. Nanog gene in goat fibroblast cells using lentiviral based expression system. Molecular Biology Reports. Jagan, Mohanrao and Atreja, S.K. DOI 10.1007/s11033-013-2931-6 ( 2013) Cryoprotective effect of cysteine on cryopreservation associated protein tyrosine Vinesh, P. V., Brahma, B., Kaur, R., Datta, T. K., phosphorylation in buffalo spermatozoa. Indian J. Goswami, S. L. and De, S. (2013). Characterization Ani. Sci., 83 (3): 247–250. of B-casein gene in Indian riverine buffalo. Gene. 2013; 527(2): 683 - 88. Kapila, R., Sebastian, R., Varma, V.P. D., Sharma, R., Kapasiya, M., Salingati, V., Kapila, S. Yadav, A., Singh, K. P., Singh, M. K., Saini, and Dang, A. K. (2013) Comparison of innate N., Palta, P., Manik, R. S., Singla, S. K., Upadhyay, immune activation after prolonged feeding of R. C. and Chauhan, M. S. (2013). Effect of milk fermented with three species of Lactobacilli. Physiologically Relevant Heat Shock on Microbiology and Immunology, 57 : 778–84. Development, Apoptosis and Expression of Some Genes in Buffalo (Bubalus bubalis) Embryos Kanyaiya, M., Sawale, P. D., Arora, S., Kapila, S. Produced In Vitro. Reproduction in Domestic and Singh, R. R. B. (2013) In vivo, effect of herb Animals: Apr 15. doi: 10.1111/rda.12175. (Withania somnifera) on immunomodulatory and antioxidative potential of milk in mice. Zandi, M., Muzaffar, M., Shah, S. M., Kaushik, Food and Agril. Immunology, http://dx.doi.org/ R., Singh, M. K., Palta, P., Singla, S. K., Manik, R. 10.1080/09540105. 2013. 837032. S. and Chauhan, M. S. (2013). WNT3A signaling pathway in buffalo (Bubalus bubalis) embryonic Kaushik, R., Sachdeva, B., Arora, S., Kapila, stem cells. Reproduction, Fertility and Development: S., Wadhwa, B.K. (2013).Bioavailability of May 9. doi.org/10.1071/RD13084. vitamin D2 and calcium from fortified milk. Food Chemistry, http://dx.doi.org/10.1016 ANIMAL BIOCHEMISTRY /j.foodchem.2013.09.150.

Research Papers Kumar, R., Singh, V. K. and Atreja, S. K. (2014) Behera, P., Kumar, R., Sandeep, I.V.R., Kapila Glutathirone-s- transferase Role in buffalo (Bubalus R., Dang, A. K., and Kapila, S. (2103) Casein bubalis) capacitation and cryopreservation. hydrolysates enhance osteoblast proliferation and Theriogenology, 81(4):587-89. differentiation in mouse bone marrow culture. Food Kolli, V., Upadhyay, R.C., Singh D. (2013) Biosci., 2: 24–30. Peripheral blood leukocytes transcriptomic Dang, A. K., Mukherjee, J., Jamwal, M., Singh, S., signature highlights the altered metabolic pathways Mohanty, A.K., Prasad Shiv, Kapila, S. and Kapila, by heat stress in zebu cattle. Res. Vet. Sci., 2013 Dec R. (2013) Isolation of exfoliated somatic cells from 9. pii: S0034-5288(13)00381-0. buffalo milk. BuffaloBulletin, 32 (1) : 53-58. Meena, S., Rajput, Y. S. and Sharma, R. (2014) Garsa, A. K., Kumariya, R., Kumar, A., Sood, S. Comparative fat digestibility of goat, camel, cow K. and Kapila, S. (2013).Bacteriocins production and buffalo milk. International Dairy J., 35: 153-56. and different strategies for their recovery and purification. Probiotics and Antimicrobial Proteins, Onteru, S. K., Gorbach, D. M., Young, J. M., Garrick, DOI 10.1007/s12602-013-9153-z D. J. Dekkers, J. C. and Rothschild, M. F. (2013) Whole-genome association studies for residual Haq, M.R.U., Kapila, R., Sharma, R., Saliganti, V. feed intake and related traits in pig. PLoS One, 8: and Kapila, S. (2014) Comparative evaluation of e61756. cow β-casein variants (A1/A2) consumption on

ndri annual report 2013-14 report ndri annual Th2-mediated inflammatory response in mouse Onteru, S. K and Singh, D. (2013) PPAR-ϒ: A gut . European J. Nutri., ( DOI 10.1007/s00394-013- master metabolic nuclear receptor. J Endocrinol 108 0606-7) 1-11. Reprod, 17: 1-18 (Review). –– Publications ––

Onnureddy, K, Vengalrao, Y., Mohanty T. K. Vedantam, S., Rani, R., Garg, M. and Atreja, S. K. and Singh, D. (2013) Metagenomic Analysis of (2014) Localization of angiotensin-II type 1(AT1) Uterine Microbiota in Postpartum Normal and receptors on buffalo spermatozoa:AT1 receptor Endometritic Water Buffaloes (Bubalus bubalis). J. activation during capacitation triggers rise in cyclic Buffalo Sci., 2 : 124-34. AMP and calcium. Molecular Biology Reports .Jan 17(Equp ahead of print). Pothuraju, R., Sharma, R. K., Chagalamarri, J., Jangra, S. and Kumar Kavadi, P. (2013), A Zhao, X., S., Onteru, M. Saatchi, D. Garrick, M. systematic review of Gymnema sylvestre in obesity Rothschild. 2013. A genome wide association study and diabetes management. J. Sci. in Food and Agri., (GWAS) for canine cryptorchidism in Siberain doi: 10.1002/jsfa.6458 Huskies. J. Anim. Breed. Genetic, doi: 10.1111/ jbg.12064. [Epub ahead of print]. Ros-Freixedes, R., Sadler, L. J., Onteru, S. K., Smith, R. M., Young, J. M., Johnson, A. K., Lonergan, S. Popular Articles Huff-Lonergan, M., E., Dekkers,,J. C. Rothschild, M. F.( 2013) Relationship between gilt behavior and Lata, K., Naik, L., Sharma, R. and Rajput, Y. S. (2013) meat quality using principle component analysis. Lateral Flow Assay - Concept and its Applications Meat Sci., 96: 264-69. in Food Analysis. Indian Food Industry, 32 (5): 22-32.

Sawale, P.D., Singh, R.R.B., Kapila, S., Arora, Naik, L., Lata, K., Sharma, R., Rajput, Y. S. and Abhisek (2013) Health implications and the science S., Rastogi, S. and Rawat, A.K.S. (2013) behind A1 and A2 milk. Food and Beverage News, Immunomodulatory and antioxidative potential of 16-31: 27-30. herb (Pueraria tuberosa) in mice using milk as the carrier. International J. Dairy Tech.., 66 (2): 202-06. Naik L., Sharma R., Rajput Y. S. and Manju G. (2013) Application of High Pressure Processing Shandilya, U. K., Kapila, R., Singh S., Dahiya D., Technology for Dairy Food Preservation - Future Kapila S. and Kansal V. K. (2013) Induction of Perspective: A Review. J. Anim. Prod. Adv., 3(8): immune tolerance to caseins and whey proteins 232-41. by oral intubation in mouse allergy model. J. Anim. Phy. and Anim. Nutr., DOI: 10.1111/jpn.12092. Sharma, R., Rajput, Y. S. and Barui, A. K. (2013) Rapid methods for detection of adulteration in milk Sharma, R., Kapila, R Raies Ul Haq M., Salingati, ., – present status. Indian Dairyman, 65 (4): 99-102. V., Kapasiya, M. and Kapila, S. (2013) Age- associated aberrations in mouse cellular and Book Chapters humoral immune responses. Aging Clinical and Experimental Res., DOI 10.1007/s40520-013-0190-y. Nayan, V., Onteru, S. K., Singh, D. (2013) Genomic technologies: A way forward for learning climate Sharma, R., Kapila, R., Kapila, S. (2013). Probiotics resilience through cellular responses to heat as Anti-Immunosenescence Agents. Food Reviews stress. In “Climate Resilient Livestock and Production International, 29:201–16. System”, Edited by Singh, S.V., Upadhyay, R. C., Sirohi, S., Singh, A.K. Published by National Dairy Singh, D., Sharma, I., Onteru, S. K. (2013) Fuel Research Institute, Karnal, Haryana, Chapter 16: Sensor PPARγ: a Potential Gateway for fertility 177-84. regulation in buffalo. Buffalo Bulletin, 32: 204-17. Nayan, V., Onteru, S. K., Singh, D. (2014) Epigenetics: Singh, V. K., Kumar, R., and Atreja, S. K. (2014) A promising paradigm for controlling fertility Cryo-survival, cryo-capacitation and oxidative in dairy animals. In “Contemporary Topics in Life stress assessment of buffalo spermatozoa Sciences”, Edited by: P.P. Mathur, Copyright © 2014, cryopreserved in new Soya Milk Extender. Narendra Publishing House, Chapter 4: 53–74. Livestock Sci., (1) In Press.

Sirohi, S. K., Chaudhary, P. P., Singh, N., Singh, DAIRY CHEMISTRY D., Puniya, A. K. (2013) The 16S rRNA and mcrA Research Papers gene based comparative diversity of methanogens 2013-14 report ndri annual in cattle fed on high fiber based diet. Gene, 10:523 Anupmarani, Sharma, Vivek, Arora, Sumit, Lal, (2): 161-6. Darshan and Kumar, Anil (2013) A rapid reversed- 109 –– Publications ––

phase thin layer chromatographic protocol for - Food Sci. and Tech.. 56: 421 - 26 http://dx.doi. detection of adulteration in ghee with vegetable org/10.1016/j.lwt. 2013.11.029. oils. J. Food Sci. Technol., ( DOI 10.1007/s13197- 013-1208-3). Mann, B. Kumari, A., Prajapati, K., Kumar, R. and Sharma, R. In vitro and in vivo assessment of the Arora, Sumit, Shendurse, Ashish M., Sharma, antioxidant activity of whey protein hydrolysates Vivek, Wadhwa, B. K. and Singh, A. K. (2013) prepared using commercial enzymes. J Anim. Sci. Assessment of stability of binary sweetener blend Vol. 91: E-Suppl. 2/J. Dairy Sci. Vol. 96: E-Suppl. (aspartame x acesulfame-K) during storage in 1 : 396. whey lemon beverage. J. Food Sci. and Tech., 50 (4) : 770–76. Meena, S., Rajput, Y. S. and Sharma, R. (2014) Comparative fat digestibility of goat, camel, cow Barui, A.K., Sharma, R., Rajput, Y. S. and Singh, S. and buffalo milk. International Dairy J., 35 : 153-56. (2013) A rapid paper chromatographic method for detection of anionic detergent in milk. J. Food Sci. Naik, L., Mann, B., Bajaj, R., Sangwan, R.S. & Tech.., 50: (4) : 826-29. and Sharma, R. (2012) Process optimization for the production of bio-functional whey Gupta, Aparna, Mann, Bimlesh, Kumar, Rajesh and protein hydrolysates: adopting response surface Bhagat, Ram, Wan, Sang (2013) “ACE-Inhibitory methodology. Int. J. Peptide & Therapeutics, 19: Activities of Cheddar Cheeses made with Adujuct 231 - 37. Cultures at Different Stages of Ripening.” Adv. Dairy Res.1:102, 2-6 Doi/0.4172/adv.1000102. Patel, Shriyesh, Shende, Sneha, Arora, Sumit, and Singh, A. K. (2013) Assessment of the anti-oxidant Kumar, Naveen and Seth, Raman (2013) potential of coriander extracts in ghee when stored Determination of melamine in milk by reversed at high temperature and during deep fat frying. phase high – performance liquid chromatography International J. Dairy Tech., 66 (2) 207-13. with diode array detection. Indian. J. Dairy. Sci., 66 (5) 388 – 92. Sari, T. P., Mann, Bimlesh, Sharma, Rajan, Kumar, Rajesh, Vikrant, Minaxi (2013) Process optimization Kanthale, Prashant, Kumar, Anil, Upadhayay, for the production of nanoencapsulated curcumin Neelam, Lal, Darshan, Rathod, Gopal and Sharma, and analysis for physico-chemical characteristics Vivek (2013) Qualitive Test for the detection of and antioxidant mechanism. International J. Biotech. extraneous thiocyanate in milk. J. Food Sci. & Tech., and Bioengineering Res., 4: 581-86. DOI 10-1007/s 13197-013-1174-9. Sawale, Pravin Digambar, Singh, Ram Ran Karuna, Meghwal, Sharma, Vivek, Lal, Darshan Bijoy, Kapila, Suman, Arora, Sumit, Rastogi, and Arora, Sumit (2013) Effect of cholesterol Subha and Rawat, Ajay Kumar Singh (2013) removal on granualation behavior of low- Immunomodulatory and antioxidative potential cholesterol ghee. International J. Dairy Tech., 66 (1) of herb (Pueraria tuberosa) in mice using milk as the : 98 -102. carrier. International J. Dairy Tech., 66 (2) : 256 - 63.

Kaushik, Ravinder, Sachdeva, Bhawana, Arora, Popular Articles Sumit and Wadhwa, B. K. (2014) Development of an analytical protocol for the estimation of vitamin Gandhi, Kamal, Kumar, Anil, Gosewade, Saurabh,

D2 in fortified toned milk.Food Chemistry 151: 225- Kaushik, Ravinder, Lal, Darshan (2013) X-Ray 230 10.1016/j.foodchem.2013.11.085. Crystallography and Its Applications in Dairy science: A Review. Research and Reviews: J. Dairy Kaushik, Ravinder Sachdeva, Bhawana, Arora, Sci. and Tech., 2 (1):21-23.ISSN: 2319-3409. Sumit, Kapila, Suman and Wadhwa, B. K. (2014)

Bioavailability of vitamin D2 and calcium from Gandhi, Kamal, Sarkar, Prabin, Kumar, Anil, Lal, fortified milk.Food Chemistry 147: 307-311.10.1016/ Darshan (2013) “MALDI-TOF MS: Applications in j.foodchem.2013.09.150. Dairy and related sectors”. Research and Reviews: J. Dairy Sci. and Tech., 2(2): 19-27.ISSN:2319 – 3409.

ndri annual report 2013-14 report ndri annual Kaushik, Ravinder, Sachdeva, Bhawana and Arora, Sumit (2014) Vitamin D2 stability in fortified milk Gandhi, Kamal, Kumar, Anil, Sarkar, Prabin, Lal, 110 during processing, packaging and storage LWT Darshan (2013) “NALDI-TOF MS: Applications in –– Publications ––

Dairy and related sectors”. Research and Reviews: J. Anuj Kumar, Patel, A. A., Singh, R. R. B. and Desai, Dairy Sci. and Tech., 2 (2) : 28 - 36. ISSN: 2319 – 3409. K. (2013) Alkali pre-soaking effects on acridity, colour parameters and oxalate content of elephant Gandhi, Kamal, Sarkar, Prabin, Aghav, Ashok, foot yam. J. Root Crops, 39(1): 88-95. Hazra, Tanmay, Lal, Darshan (2013) “Modified milk fat and its applications in Food products” Chopde, S., Kumar, B., Minz, P. S. and Sawale, P. Research and Reviews: J. Dairy Sci. and Tech., 2(3):16- (2013) Feasibility study for mechanized production 24. ISSN: 2319 – 3409. of Rabri. Asian J. Dairying and Foods Res., 32(1): 30-34. Goyal, Ankit, Sharma, Vivek, Upadhyay, Neelam, Gill, Sandeep, Sihag, Manvesh ( 2014) Flax and Giri, A. and Kanawjia, S. K. (2013) Estimation of flaxseed oil: an ancient medicine & modern production cost for omega-3 fatty acid incorporated functional food. J Food Sci. Technol. (DOI 10.1007/ processed cheese spread. International J. Sci. and s13197-013-1247-9). Res., 2(11): 278-82.

Lata, K., Naik, L., Sharma, R. and Rajput, Y. S. (2013) Giri, A., Kanawjia, S. K. and Khetra, Y. (2013) Lateral flow assay - concept and its applications in Textural and melting properties of processed food analysis. 32 (5), Sept.-Oct. : 22-32. cheese spread as affected by incorporation of different inulin levels. Food and Bioprocess Tech., Naik L., Sharma R., Rajput Y. S. and Manju, G. DOI: 10.1007/s11947-013-1235-0 (Published online) (2013) Application of high pressure processing technology for dairy food preservation - future Giri, A., Kanawjia, S. K. and Rajoria, A. (2014) perspective: A Review. J Anim Prod Adv., 3 (8): Effect of phytosterols on textural and melting 232-41. characteristics of cheese spread. Food Chemistry, 157: 240-45. Naik, L., Lata, K., Sharma, R. Rajput, Y. S. and Abhisek (2013) Health implications and the science Goyary, J., Owary, K. R., Basumatary, D., behind a1 and a2 milk. Food and Beverage News, Mushahary, N. M., Marumalla, T., Meena, G. 16(31): 27-30. S. and Kumar, N. (2013) Analyses of Synbiosis between Lactobacillus Bulgaricus and (Oat & Onion) Mann, Bimlesh, Sharma, Rajan, Kumar, Rajesh, Minaxi, and Sari, T. P. (2013) Nanoencapsulation Prebiotics. International J. Biotech. and Bioengineering of functional ingredients for their delivery in Res., 4 (5):445-50. functional dairy foods. Indian Dairyman, April 13. Gupta, H. R, Kanawjia, S. K., Salooja, M. K., 165-69. Devaraja, H. C. and Rajoria, A. (2013) Process optimization for the production of whey protein DAIRY TECHNOLOGY enriched dairy drink. Asian J. Dairy and Food Res., 32(4): 257-65. Research Papers

Babu, D., Verma, N. K. and Raju, P. N. (2013) Khatkar, S. K. and Gupta, V. K. (2013) Procurement cost of milk and milk products in Physicochemical and functional quality attributes organized sector of Tamil Nadu – A comparative of dairy whitener prepared from ultrafiltration analysis. Indian J. Dairy Sci., 66(2): 149-62. process. J. Food Processing and Preservation. doi: 10.1111/jfpp.12074. Divya, K. B., Sathish Kumar, M.H., Thompkinson, D. K. and Sabikhi, L. (2013) Sensory attributes, Khatkar, S. K., Gupta, V. K. and Khatkar, A.B. fatty acid profile and oxidative stability of low (2014) Studies on preparation of medium fat liquid fat buffalo milk supplemented with omega 3 dairy whitener from buffalo milk employing fatty acids and dietary fiber. Indian J. Dairy Sci., ultrafiltration process. J. Food Sci. and Tech., 66(6):469-76. DOI:10.1007/s13197-014-1259-0.

Ganguly, S. and Sabikhi, L. (2013) Effect of heat Makhal, S., Kanawjia, S. K. and Giri, A. (2013) A

treatment on nutritional profile of a composite dual-acidification process for the manufacture 2013-14 report ndri annual dairy-cereal substrate. Short Communication. of direct-acidified Cottage cheese. International J. Indian J. Dairy Sci., 66(6):545-47. Dairy Tech., 66(4): 552-61. 111 –– Publications ––

Mandal, S., Hati, S., Puniya, A. K., Khamrui, K. nutraceuticals: Food applications and interactions and Singh, K. (2014) Enhancement of survival of with food components. Critical Reviews in Food Sci. alginate-encapsulated Lactobacillus casei NCDC and Nutr., DOI: 10.1080/10408398.2011.649148. 298. J. Sci. of Food and Agri., Published online. DOI 10.1002/jsfa.6514. Raju, P. N. Ganguly, S. and Priyanka (2013) Antimicrobial food packaging materials. Food Meena, G.S., Kumar, N., Majumdar, G. C., Marketing and Tech., 4(5):40-42. Banerjee, R., Meena, P. K., and Yadav, V. (2014) Growth Characteristics Modeling of Lactobacillus Prasad, R., Jha, Alok, Sabikhi, L. and Kumar, A. and Acidophilus using RSM and ANN. Brazilian Unnikrishnan, V.S. (2013). Nutritional advantages Archives of Biology and Technology, 57 (1): 5-22. of oats and opportunities for its processing as value added foods - a review. J. Food Sci. and Tech.. DOI: Narayana, N. M. N. K. and Gupta, V. K. (2013) 10.1007/s13197-013-1072-1. Physical, textural and sensory attributes of plain set yoghurt made employing ultrafiltration technique Rao, K. H., Raju, P. N., Reddy, G.P. and Hussain, as affected by titratable acidity during incubation. S.A. (2013) Public-private-partnership and value Biology, Agricultural and Environmental Sciences. addition: A two pronged approach for sustainable Proceedings of 3rd International Symposium of dairy supply chain management. J. Supply Chain South Eastern university of Sri Lanka, 6-7 July 2013, Management, 10 (1): 15-25. Oluvil, Sri Lanka. pp : 19-24. Sathsih Kumar, M.H., Saxena, D. and Sabikhi, L. Narayana, N. M. N. K. and Gupta, Vijay K. (2013) (2013) Developments in whey-based beverages – a Effect of total milk solid content adjusted by adding review. Indian J. Dairy Sci., 66(4):281-87. ultrafiltered milk retentate on quality of cow milk set mango yoghurt. International J. Dairy Tech., 66(4) : 570-75. DAIRY MICROBIOLOGY

Parmar, P., Khamrui, K., Devaraja, H. C. and Singh, Research Papers R. R. B. (2013) The effects of alcoholic extract of Behare, P. V., Singh, R., Nagpal, R. And Rao, K. H. Arjuna (Terminalia arjuna Wight & Arn.) bark on (2013) Exopolysaccharides producing Lactobacillus stability of clarified butterfat. J. Medicinal Plants fermentum strain for enhancing rheological and Res., 7(35): 2545-50. sensory attributes of low-fat Dahi. J Food Sci. Technol., 50: 1228-32. Sawale, P.D., Singh, R.R.B. and Arora, S. (2013). Stability and quality of herb (Pueraria Tuberosa)- Chandran, A., Duary, R. K., Grover, S. and Batish, milk model system. J. Food Sci. and Tech., 1-7. V. K. (2013) Relative expression of bacterial and host specific genes associated with probiotic Saxena, D., Chakraborty, S. K., Sabikhi, L. and survival and viability in the mice gut fed with Singh, D. (2013) Process optimization for a nutritious low-calorie high-fiber whey-based Lactobacillus plantarum Lp9. Microbiol. Res.,168 : ready-to-serve watermelon beverage. J. Food 555-62. Sci. and Tech., DOI: 10.1007/s13197-013-1066-z. Grover, S., Sharma, V. K., Mallapa, R.H. and Batish, Published Online: June 25. V.K (2013) Draft genome sequence of lactobacillus fermentum Lf1, an Indian isolate of human gut Popular Articles origin. Genome Announc., 1(6). pii: e00883-13. doi: Hati, S., Prajapati, J. B., Mandal, S., Khamrui, K. 10.1128/genomeA.00883-13. (2013) Biofunctional whey-based beverages. Indian Dairyman. 65 (5) 62-69. Grover, S., Sharma, V. K, Mallapa, R. H. and Batish, V.K. (2013) Draft genome sequence of lactobacillus Hati, S., Mandal, S., Khamrui, K., Prajapati, J. B plantarum strain Lp91, a promising Indian probiotic (2013) Microencapsulation of probiotic cultures for isolate of human gut origin. Genome Announc., 1(6). preparation of yoghurt. International J. Fermented pii: e00976-13. doi: 10.1128. Foods. 2 (1) 47-61.

ndri annual report 2013-14 report ndri annual Hati, S, Vij, S., Brij, S., Kumari, V. and Mandal, Hussain, S.A., Raju, P. N., Singh, R.R.B. and S. (2013). Antioxidative activity and polyphenol 112 Patil, G.R. (2013). Potential herbs and herbal content in fermented soy milk supplemented with –– Publications ––

WPC-70 by probiotic lactobacilli. Int. Food Res. J., characteristics of shrikhand. Indian J. Dairy Sci., 20(5): 2125-2131. 66(1) : 11-21.

Iyer, R. and Tomar S. K. (2013) Determination of Renuka, Puniya, M., Sharma, A., Malik, R. K., folate/ folic acid level in milk by microbiological Upadhyay, R. C., Puniya, A. K. (2013) Influence assay, immuno assay and high performance liquid of pediocin and enterocinon in-vitro methane, chromatography. J. Dairy Res., 80: 233–239. gas production and digestibility. Intl. J. Current Microbiol Appl. Sci., 2(11):132-42. Kumar, A., Grover, S and Batish, V.K. (2013) Application of multiplex PCR assay based on uidR Singh, N. A., Kumar N., Raghu, H.V., Sharma, P.K., Singh, V.K., Khan, A. and Raghav, N. (2013) and flicH7genes for detection of Escherichia coli 0157:H7 in milk. J Gen. Appl. Microbiol., 59: 11-19. Spore inhibition based enzyme substrate assay for

monitoring of Aflatoxin 1M in milk. Toxicological & Kumar, R., Grover, S., Kaushik, J. K. and Batish, Environmental Chemistry, 95 (5): 755-77. V.K. (2013) IS30-Related transposon mediated insertional inactivation of bile salt hydrolase Singh, T. P., Malik, R. K., Kaur, G and Renuka. (bsh1) gene of lactobacillus plantarum strain Lp20. (2014) Safety assessment and evaluation of Microbiol. Res., http://dx.doi.org/10.1016/j. probiotic potential of Lactobacillus reuteri strains micres.2013.10.006. under in vitro conditions. Int. J. Curr. Microbiol. Appl. Sci., 3(2): 335-48. Kumar, S., Dagar, S. S., Puniya, A. K. and Upadhyay, R. C. (2013) Changes in methane Sudhakaran, A. V., Panwar, H., Chauhan, R., Duary, R. K., Rathore, R. K., Batish, V. K. and Grover, S. emission, rumen fermentation in response to diet (2013) Modulation of anti-inflammatory response and microbial interactions. Res. Vet. Sci., 94:263–68. in lipopolysaccharidestimulated human THP-1 cell Kumar, S., Dagar, S. S., Puniya, A. K. and line and mouse model at geneexpression level with Upadhyay, R. C. (2013) Microbial profiles,in vitro indigenous putative probiotic lactobacilli. Genes gas production, dry matter digestibility based on Nutr., 8 : 637–48. various ratios of roughage to concentrate. Ann Thakur G., Yadav A., Tehri N., Kumar N., Raghu Microbiol., 63 : 541–45. H. V., Singh N. and Singh V. K. (2013) Rapid and novel microscopy technique to detect germination Kumar., S., Raghu., H. V., Kumar., N., Singh, N. A. initiation and specificity in Bacillus spores. Int. J. and Malik, R. K. (2013) Spore based chromogenic Res.in Pure and Appl. Microbiol., 3(4): 134-38. assay for detection of β-lactam antibiotic in milk. Indian J. Dairy Sci., 66 : 6. Verma, N., Singh, N.A., Kumar, N., Singh, V.K. and Raghu, H.V. (2013) “Development of “field Mandal, S., Hati, S., Puniya, A. K., Khamrui, K. level” chromogenic assay for aflatoxin M detection and Singh, K. (2013) Enhancement of survival of 1 in milk.” Adv. Dairy Res.,1: 2. doi: 10.4172/2329- alginate encapsulated Lactobacillus casei NCDC 888X.1000108. 298. J. Sci. Food and Agri., DOI: 10.1002/jsfa.6514. Verma, N., Singh, N. A., Kumar N. and Raghu, Mandal, S., Hati, S., Puniya, A. K., Singh, R., Singh, H. V. (2013). Screening of different media for K. (2013) Development of synbiotic milk chocolate sporulation of Bacillus megaterium. Int. J. Microbiol. using encapsulated Lactobacillus casei NCDC 298. Res. and Reviews, 1 (4): 68-73. J. Food Process Preserv, 37(5):1031-37. Yadav, A.K., Tyagi, A., Kaushik, J.K., Saklani, A.C., Nanda, D. K., Singh, R., Tomar, S. K., Dash, S. Grover, S. and Batish, V.K. (2013). Role of surface K., Jayakumar, S., Arora, D. K., Chaudhary, R. layer collagen binding protein from indigenous and Kumar, D. (2013) Indian chilika curd – A Lactobacillus plantarum 91 in adhesion and its anti- potential dairy product for geographical indication adhesion potential against gut pathogen. Microbiol registration. Indian J. Traditional Knowledge, 12 (4): Res., 168 : 639-45. 707-13. Review Articles

Rejeesh R., Tomar., S. K., Singh, R. R. B. and Singh, 2013-14 report ndri annual A. K.. (2013) The role of mixed starter cultures in Grover, S., Rashmi, H.M. and Batish, V.K. enhancing the technotextural and microstructure (2013) Probiotics for human health new 113 –– Publications ––

innovations and emerging trends. Encyclopedia Hati, S., Mandal, S. and Prajapati, J. B. (2013) Novel of Metagenomics. (Editor: Karen E. Nelson) starters for value added fermented dairy products. http://www.springerreference.com/docs/html/ Curr. Res. Nutr. Food Sci., 1(1): 83-91. chapterdbid/304441.html Kumar, N., Thakur, G., Raghu, H. V., Singh, N., Hati, S., Mandal, S., Khamruim, K. and Prajapati, Sharma, P. K., Khan, A., Balhara, M., Avinash, J.B. (2013) Microencapsulation of probiotic cultures Lawaniya, R., Kouser, S., Tehri, N., Gopaul, Rajesh for preparation of yoghurt. Int. J. Fermented Foods, and Shivani, Arora (2013) bacterial spore based 2(1): 47-61. biosensor for detection of contaminants in milk. J. Food Process Technol., 4: 277. doi:10.4172/2157- Kumar, N., Thakur, G., Raghu, H.V., Singh, N., 7110.1000277. Sharma, P.K., Khan, A., Balhara, M., Avinash, Malik, R. K., Garg, S. and Singh, T. P. (2014) Lawaniya, R, Kouser, S., Tehri, N., Rajesh, G and Probiotics in combating life style disorders. Indian Arora, S. (2013) Bacterial Spore Based Biosensor for Dairyman, 66(1): 138-41. Detection of Contaminants in Milk. J. Food Process Technol., 4 : 277. doi:10.4172/2157-7110.1000277 Singh, T. P., Kaur, G., Malik, R. K. and Garg, S. (2013) Reinforcement of intestinal epithelial barrier Kumar, S., Choudhury, P. K., Carro, M. D., Griffith, by probiotics and their extracellular proteins. Int. G. W., Dagar, S. S., Puniya, M., Calabro, S., Ravella, J. Adv. Res., 1(10): 409-14. S. R., Dhewa, T., Upadhyay, R. C., Sirohi, S. K., Kundu, S. S., Wanapat, M. and Puniya, A. K (2014) . Book Chapters New aspects and strategies for methane mitigation from ruminants. Appl. Microbiol. Biotechnol., 98: Behare, P. V., Singh, R., Singh, R.P., Mohanty, A. 31-44. K., Kumar, S., Mondal, S., Tomar, S. K., Sharma, Y. and Panikar, A. (2013) Natural Biothickener of Pandey, N., Malik, R.K., Kaushik, J. K., Singroha, G. Lactic Acid Bacteria: Alternative for Commercial (2013) Gassericin A: a circular bacteriocin produced Additives. In: Dairy and Food Processing Industry: by lactic acid bacteria Lactobacillus gasseri. World J Recent Trends (Part I), edt. Mishra, B.K., BIOTECH ® Microbiol Biotechnol., 29(11) : 1977-87. BOOKS , New Delhi, pp: 105-14.

Panwar, H., Rashmi, H. M., Batish, V. K. and Grover, Dahiya, M., Vij, S. and Hati, S. (2013) Biofuels: S. (2013) Probiotics as potential biotherapeutics in Current Trends and Future Prospects” IN: Dairy the management of type 2 diabetes – Prospects and and Food Processing Industry Recent Trends— Part I —Published by: Biotech Books New Delhi, Perspectives. Diabetes Metabolism Res. and Reviews, pp: 77. 29: 103-12. DOI: 10.1002/dmrr.2376. Dagar, S. S., Kumar, S., Mudgil, P., Puniya, M., Sharma, P., Tomar, S. K., Goswami, P., Sangwan, Sirohi, S. K., Sehgal, J. P., Kundu, S. S., Puniya, A. V. and Singh, R. (2014) Antibiotic resistance among K. (2014) Anaerobic rumen fungi for the benefits of commercially available probiotics. Food Res. Int., livestock. In: Microbes in the Service of Mankind: 57 : 176–95. Tiny Bugs with Huge Impact. Eds: Nagpal, R., Kumar, A. and Singh, R.; I.K. International Singh, B. P., Vij, S. and Hati, S. (2014). Review- Publishers, New Delhi, pp: 125-39. Functional significance of bioactive peptides derived from soybean. Peptides, 54 : 171–79. Hati, S., Vij, S., Mandal, S., Khamrui, K. and Prajapati, J. B. (2014) Utilization of soy Thakur, G., Kumar, N., Raghu, H. V., Khan, A., oligosachharides during fermentation by Yadav, A., Singh, N. and Singh, V.K. (2013). Spore Lactobacilli in Oligosachharides: food sources, based biosensors for detection of contaminants in biological roles and health implications. Nova milk: A review. Int. J. Dairy Sci. Res., 2(2): 15-21. Science Publishers Inc. NY pp: 233.

General Articles Hati, S., Vij, S. and Mandal, S. (2013) Microbiology of Dahi, Lassi and Yoghurt. In: Dairy and Food

ndri annual report 2013-14 report ndri annual Garg, S., Malik, R. K., Singh, T. P and Renuka Processing Industry: Recent Trends (Part I), Ed. (2014) Child nutrition: a pillar to development. Int. Mishra, B.K., BIOTECH BOOKS®, New Delhi, pp: 114 J. Adv. Res., 2(1): 766-72. 231-47. –– Publications ––

Hati, S., Vij, S., Mishra, B.K. and Mandal, S. (2013) characteristics of cryogenic and ambient ground Nutritional and Therapeutic Value of Fermented turmeric, Int. Agrophysics, 28 : 111-17. (doi: 10.2478/ Soy Milk. In: Dairy and Food Processing Industry: intag-2013-0033). Recent Trends (Part II), Ed. Mishra, B.K., BIOTECH BOOKS®, New Delhi, pp: 77-88. Barnwal, P., Kumar, P., Singh, K. K., Mohite, A., Saxena, S. N. and Zachariah T. J. (2014) Grinding Hati, S., Mandal, S. and Vij, S. (2013) Functional characteristics of coriander, fenugreek and black Dairy Beverages. In: Animal Products Technology, pepper under cryogenic and ambient grinding Eds. Mandal, P.K. and Biswas, A.K., Studium Press conditions. Int. J. Seed Spices, 4(1): 63-70. (India) Pvt. Ltd., New Delhi, pp: 344-53. Barnwal, P., Singh, K. K., Kumar, R. and Saxena, Kaur, M., Kumar, H., Kumar, N. and Puniya, A. K. S. N. (2014) Selected thermal properties of cryo- (2013) Recent trends in production of fermented dairy ground coriander powder (var.RCR-41). J. Spices and food products. In, “Dairy and Food Processing and Aromatic Crops, 23(1): 45-50. Industry Recent Trend” Biotech Books, Part 1:141-61. Barnwal, P., Singh, K. K., Kumar, R. and Zachariah, Mandal, S., Hati, S. and Khamrui, K. (2013). T. J. (2013) Thermal properties of cryo-ground Functional Starters for Dairy Products. In: black pepper (var. Panniyur 1). J. Spices and ANIMAL PRODUCTS TECHNOLOGY, Eds. Aromatic Crops, 22 (2): 148-53. Mandal, P.K. and Biswas, A.K., Studium Press (India) Pvt. Ltd., New Delhi, pp: 203-15. Barnwal, P., Singh, K. K., Sharma, A., Choudhary, A. K. and Saxena, S. N. (2014) Effect of grinding Manju, G., Shaik Abdul Hussain, Mishra, S. conditions on physico-chemical, thermal and K. and Ram, C. (2013) Natural antimicrobials antioxidant properties of coriander powder. Int. J. for preservation of foods. In: Dairy and Food Seed Spices, 4(1): 25-32. Processing Industry -Recent Trends (Part I) (ISBN: 978-81-7622-298-3 (Part-I) pp: 204-30. Biotech Book Barnwal, P., Singh, K. K., Sharma, A., Choudhary, Publishers, New Delhi. A. K., Zachariah, T. J. and Saxena, S. N. (2014) Biochemical, Antioxidant and Thermal Properties Ram, C. and Kumar, V. (2013) Probiotics and Herbs: of Cryogenic and Ambient Ground Turmeric Emerging treatment options for non alcoholic fatty Powder. Int. Agril. Engineering J., 23(1): 39-46. liver diseases. In: Molecular Biology of Bacteria (ISBN: 978-1-62618-251-69 (e-Book) pp: 125-42. Barnwal, P., Mohite, A. M., Singh, K. K. and Nova Science Publishers, Inc. 400 Oser Avenue, Kumar, P. (2014) Selected physico-mechanical Suite 1600 Hauppauge, NY 11788-3619 characteristics of cryogenic and ambient ground turmeric. International Agrophysics, 28: 111-17. (doi: Manuals 10.2478/intag-2013-0033).

Behare, P., Ram, C., Mandal, S. and Tomar, S. K. Barnwal, P., Kumar, P., Singh, K. K., Mohite, A., (2013) Laboratory Manual on “Starter Cultures and Saxena, S. N. and Zachariah T. J. (2014) Grinding Fermented Milk Products”, Published by Director, characteristics of coriander, fenugreek and black NDRI (Deemed University), Karnal (Publication pepper under cryogenic and ambient grinding No. NDRI 107/2013). conditions. Int. J. Seed Spices, 4(1): 63-70.

Ram, C., Mandal, S. and Behare, P.V. (2013) Barnwal, P., Singh, K. K., Kumar, R. and Saxena, Laboratory Manual on “Laboratory Techniques S. N. (2014) Selected thermal properties of cryo- for Fluid Milk and Milk Products”, Published ground coriander powder (var.RCR-41). J. Spices by Director, National Dairy Research Institute and Aromatic Crops, 23(1): 45-50. (Deemed University), Karnal (Publication No. NDRI 99/2013). Barnwal, P., Singh, K. K., Kumar, R. and Zachariah, T. J. (2013) Thermal properties of cryo-ground DAIRY ENGINEERING black pepper (var. Panniyur 1). J. Spices and Aromatic Crops, 22 (2): 148-153. Research Papers

Barnwal, P., Singh, K. K., Sharma, A., Choudhary, 2013-14 report ndri annual Barnwal, P., Mohite, A. M., Singh, K. K. and A. K. and Saxena, S. N. (2014) Effect of grinding Kumar, P. (2014) Selected physico-mechanical conditions on physico-chemical, thermal and 115 –– Publications ––

antioxidant properties of coriander powder. Int. J. Popular Articles Seed Spices, 4(1): 25-32. Chopde, S.S., Minz, P.S., Pawar, N.R., and Barnwal, P., Singh, K. K., Sharma, A., Choudhary, Changade, S.P. (2013). Application of high pressure A. K., Zachariah, T. J. and Saxena, S. N. (2014) technology for cheese processing. Beverage and Food Biochemical, Antioxidant and Thermal Properties World, 40(7) : 29-31. of Cryogenic and Ambient Ground Turmeric Minz, P.S., Dodeja, A. K. and Halder, K. (2013) Powder. International Agril. Engineering J., 23(1): Reading your food label. Food Marketing and 39-46. Technology, 4(10) : 50-52. Chopde, S., Kumar, B., Minz, P. S. and Sawale, P. Minz, P. S., Behare, P. and Devaraja, H.C. (2013) Feasibility study for mechanized production (2013) Supercritical fluid extraction of bioactive of Rabri. Asian J. Dairying & Foods Res., 32 (1): 30-34. components for use in functional foods. Beverage Dabas, J. K., Kumar, S., Dodeja, A. K. and Kasana, and Food World, 40(9) : 59-60. K. S. (2014) Modeling of Horizontal Shell and Minz, P.S., Sirohi, S., Singh, R. and Chopde, S.S. Tube Dry Expansion Refrigerant Evaporator. (2013) Indian Dairy Processing Industry: An International J. Advanced Mechanical Engineering, Overview. Beverage and Food World, 40(7) : 29-31. 4(1): 33-54. Chopde, S.S., Minz, P.S., Dhotre, A.V., and Manikantan, M. R., Barnwal, P. and Goyal, R. Changade, S.P. (2013). High pressure technology K. (2014) Drying characteristics of paddy in an for milk processing: A review. Beverage and Food integrated dryer. J. Food Sci. and Tech., DOI 10.1007/ World, 40(7):54-56. s13197-013-1250-1.

Manikantan, M. R., Barnwal, P. and Goyal, R. Book Chapters K. (2014) Drying characteristics of paddy in an Dodeja, A.K. Minz, P.S., Raju, P.N., and Dabbas, integrated dryer. J. Food Sci. and Tech., DOI 10.1007/ J.K. (2013). Dairy In: Handbook of Agricultural s13197-013-1250-1. Engineering. ICAR, New Delhi, pp. 626-48. Sawhney, I. K., Sarkar, B.C., Patil, G. R. and Sharma, H. K. (2013) Moisture Sorption Isotherms Book and Thermodynamic Properties of Whey Protein Concentrate Powder from Buffalo Skim Milk. J. Behare, P., Minz, P.S., Mandal, S., Sathish, M.H. Food Processing and Preservation, DOI: 10.1111/ and Chichudde, D.A. (2013) Fluid Milk Processing jfpp.12148. (Dairy Technology): Student Handbook, CBSE, New Delhi. Saxena, R., Soni, A., Saxena, S. N., Rathore, S. S. and Barnwal, P. (2013) Cryogenic Grinding: A Dairy Economics Statistics & Physical Technique to Retain Volatile Content in Natural Products. International J. Modern Physics: Management Conference Series, 22 : 589-92. Research Papers Saxena, R., Soni, A., Saxena, S.N., Rathore, S. S. and Chand, P. and Sirohi, S. (2013). Sustainability of Barnwal, P. (2013) Cryogenic Grinding: A Physical dairy breeding practices: empirical evidences of Technique to Retain Volatile Content in Natural semi-arid eastern zone of Rajasthan. Res. J. Animl Products. International J. Modern Physics: Conference Husbandry and Dairy Sci., 4(3): 47–50. Series, 22: 589-92.

Sharma, A. K., and Sawhney, I. K. (2013) Modelling Chand, P., Sirohi, S. and Sirohi, S.K. (2013). moisture sorption characteristics in dried acid Production and Demand Estimates of Livestock casein using connectionist paradigm vis-à-vis Feed and Fodder in Rajasthan. Indian J. Anim. classical methods. J. Food Sci. and Tech., doi: Nutri., 30(2):149–56. 10.1007/s13197-013-0981-3. Chauhan, A. K., Chandel, B. S. and Yadav, J. (2013)

ndri annual report 2013-14 report ndri annual Singh, A. K. and Dodeja, A.K. (2013) Manufacture Impact assessment of mineral mixture supplement of Basundi Using Three Stage SSHE. Indian J. Dairy to dairy animals in Haryana. Indian J. Eco. and 116 Sci., 65(3):197-07. Develop., 9(3):207–11. –– Publications ––

Chauhan, A. K., Pushpa, Singh, R., Datta, K. K results and policy implications. Indian J. Anim. and Ali, M. M. (2014) Haryana state agriculture Sci., 83(1):86–90. marketing board: A catalyst for modern marketing. Indian J. Eco. and Develop., 10(1a):7–12. Singh, S. R., Feroze, S. M, Datta, K. K. and Gupta, R. K. (2013) Economics of milk production: A Das, G. and Jain, D. K. (2013) Factors affecting the case study of Ajmer district co-operative milk price of bullocks in the organised cattle fairs of producers’ union. Indian J. Anim. Prod. and Mgmt., Rajasthan. Indian J. Agril. Eco., 68(4):594–99. 28(3-4):201–08.

Ghule, A., Chauhan, A. K, Mahajan, S. and Singh, S. R., Mahajan, S. and Datta, K. K. (2013) Nagrale, B. (2014) Analysis of marketed surplus What the future beholds for small holder dairy and disposal pattern of milk in commercial dairy farming in India? Some anecdotal evidences farms in Maharashtra. Indian J. Eco. and Develop., from organised dairy industry. Indian J. Dairy Sci., 10(1a):161–66. 66(2):142–48. Mahajan, S., Chauhan, A. K., Datta, K. K., Azad, M. S. and Sharma, V. K. (2013) Economics of milk Singh, R., Chauhan, A. K., Chandel, B. S., Dhaka, production in rural and peri-urban dairy farms J. P. and Singh, S.B. (2013) Economic impact of in Ludhiana, India. Asian J. Dairy and Food Res., integrated dairy development project in north 32(1):25–29. eastern hilly region: Micro evidences from Meghalaya. Indian J. Dairy Sci., 66(1):65–71. Paul, D., Chandel, B. S. and Ray, J. (2013) Quantity and value of milk losses due to technical constraints- Thorat, V. S. and Sirohi, S. (2013) Reviewing a case of crossbred cows in north-eastern states of demand-supply balance of foodgrains by 2020. J. India. Indian J. Agril. Eco., 68(4) : 562–70. Resources, Energy and Develop., 10(1):1–10.

Patbandha, T. K., Mohanty, T. K., Layek, S. S., Zadeh, M. N., Sirohi, S., Mondal, B. and Saxena, Kumaresana, A., Kantwa, S. C., Malhotra, R., R. (2013) Strengthening Bilateral Trade relations Ruhil, A. P. and Prasad, S. (2013) ROC analysis between India and Iran: Opportunities in Livestock of prepartum feeding time can accurately predict Sector. J. Resources, Energy and Develop., 10(1):39–50. postpartum metritis development in HF crossbred cows. J. Vety. Behavior, 8 : 362–66. Popular Articles

Sharma, A. K. and Sawhney, I. K. (2013) Modelling Chandel, B. S. and Sirohi, S. (2013) Pricing of milk moisture sorption characteristics in dried acid and milk products – An overview. NDRI News, casein using connectionist paradigm vis-à-vis 18(3):14-16. classical methods. J. Food Sci. and Tech., DOI: 10.1007/s13197-013-0981-3. Online First. Minz, P.S., Sirohi, S., Singh, R. and Chopde, S.S. (2013). Indian dairy processing industry: An Sharma, A. K., Jain, D. K., Chakravarty, A. K., overview. Beverage and Food World, 40(7):29–31. Malhotra, R. and Ruhil, A. P. (2013) Predicting economic traits in Murrah buffaloes with Sharma, A., Chauhan, A. K. and Singh, R. (2012). connectionist models. J. Indian Society of Agricultural Productivity and resource use efficiency in milk Statistics, 67(1): 1–11. (Special Issue on Artificial production in tribal area of Dhar district (Madhya Intelligence in Agriculture). Pradesh). Productivity, 53(1):1–6. Singh, S. R. and Datta, K. K. (2013) Importance of socio-economic and institutional factors in the use Books of veterinary services by smallholder dairy farmers Chauhan, A. K. and Singh, R. (2013) Milk Marketing in India. Current Science, 105:580–86. and Entrepreneurship (Student Handbook). CBSE, Singh, S. R. and Datta, K.K. (2013) Future of small New Delhi. holders in the Indian dairy sector – Some anecdotal evidence. Indian J. Agril. Eco., 68(2):182–94. Nagrale, B. and Datta, K. K. (2014) Economic

Analysis of Milk Production in Vidarbha Region of 2013-14 report ndri annual Singh, S. R., and Datta K. K. (2013) Determinants India. Lambert Academic Publishing GmbH & Co. of veterinary services in Punjab – Empirical KG, Saarbrucken, Germany. ISBN- 9783847334781. 117 –– Publications ––

Singh, R. and Bahare, P. (2013). Milk Marketing and Singh, S. R. and Datta, K. K. (2013) Futuristic outlook Entrepreneurship (Practical Manual). Central Board to ensure food security through broad-based of Secondary Education (CBSE), New Delhi. livelihood activities (Chapter 12). In: Agrarian Crisis in India. Academic Foundation, pp: 321-36. Book Chapters and e-books Singh, R., Chauhan, A. K. and Chandel, B. S. Datta, K. K. and Lalrinsangpuii (2013) Policy (2013) Performance and achievement of dairy issues for sustainable growth of agriculture and development programmes and schemes in agribusiness in the north eastern states of India north eastern region (Chapter 24). In: Singh, R., (Chapter 41). In: Singh, R., Naik, D. and Feroze, Naik, D. and Feroze, S.M. (Editors), Agri-Business S.M. (Editors), Agri-business Potentials in India: Potentials in India: Experiences from Hill states. EBH Experiences from Hill States Singh. EBH Publishers Publishers (India), Guwahati, pp: 318-327. ISBN: (India) Guwahti. ISBN: 9789383252213, pp: 510-26. 9789383252213.

Paul, D. and Chandel, B.S. (2014) Prioritization of Singh, R. (2013) Dairy enterprise for sustainable constraints for improving milk production potential livelihood in north east hilly region (Chapter in north-eastern states (Chapter 27). In: Singh, R., 2). In: Sharma, P.K. and Dwivedi, S. (Editors), Hill Naik, D. and Feroze, S.M. (Editors), Agri-Business Agriculture Economics and Sustainability. New India Potentials in India-Experiences from Hill States. EBH Publishing Agency, New Delhi, pp: 13-30. ISBN: Publishers (India) Guwahati, India, pp: 339-52. 9789381450871. Sharma A. K. (2014) SAS programming essentials for scientific computing in dairy sciences. In: Dairy Extension Data Analysis in Dairy Sciences Research Using SAS. (Chapter 8). Available online at SSCNARS Research Papers Website at: http://www.iasri.res.in/sscnars/ Babu, G. P., Kadian, K. S., Sankhala, G., Kumar, N. content_dairy.htm S. and Pothuraju, R. (2013) Constraints perceived Sharma, A. K. (2013) SAS programming essentials by dairy farmers in accessing ICT-enabled village for statistical computing in dairy research. In: Data information centres of I-Kisan in Andhra Pradesh. Analysis in Dairy Sciences Research using SAS. Indian Indian J. Dairy Sci, 66 (5): 441-45. Agricultural Statistics Research Institute, Indian Agricultural Statistics Research Institute (ICAR), Babu, G. Prasad, Kadian, K. S., Kumar, R. Senthil New Delhi-110012, India. Available online: http:// and Subash, S. (2013) Use of ICT Enabled Services www.iasri.res.in/sscnars/dairysci/ 8.pdf/. for Empowerment of Dairy Farmers in Andhra Pradesh. J. Global Communication 6(2):103-08. Sharma, A. K. (2013) Connectionist approach to predictive modelling in dairy and food sciences. Chand, Subash, Meena, B. S., Kadian, K. S. and In: Paul, R.K., et al. (Editors) Recent Advances in Singh Anoop (2013) Knowledge of dairy farmers Statistical Modelling Techniques. Indian Agricultural on productive disorders of dairy animals. Vety. Statistics Research Institute, New Delhi - 110012, Practitioner, 14(1): 23-25. India. Available online: http://iasri.res.in/cbp/ ebook22.aspx?trainingApprovedId=CA FT-2013- Chand, Subhash., Meena, B. S. and Verma, Hukum 237&trainingTitle = Recent +Advances+in+Statis Chandra (2014) A study on farmers’ satisfaction tical+Modelling+Techniques with delivery of Veterinary services. Indian J. Anim. Res., 48 (1) : 67-70. Sharma, A.K. (2013) Recommender systems for Internet computing – An agricultural perspective. Chand, Subhash., Meena, B. S., Kadian, K. S. and In: Arora, A., et al. (Editors) Development of Web Singh, Anoop (2013) Knowledge of Dairy farmers Application for Agricultural Information Management, on reproductive disorders of dairy animals. Vety. pp: 321-326. Indian Agricultural Statistics Research Practitioner, 14(1) : 23-25. Institute (ICAR), New Delhi-110012, India. Available online: http://iasri.res.in/cbp/ Chand, Subhash, Meena, B. S., Kumar, Ashok, ebook22.aspx?trainingApprovedId=WS-2013- Yaday, S. P. and Soni, S. K. and Singh, Anoop

ndri annual report 2013-14 report ndri annual 191&training Titl e=Development%20of%20 (2013) Incidence of reproductive disorders of Web%20Application%20for%20Agricultural% dairy animals of Rajasthan. The Indian Vety. J.. 90 118 20Information%20 Management (3): 36-39. –– Publications ––

Datt, R., Jha, S. K. and Salaria, N. (2013) Farmers in Tamil Nadu Indian J. Anim. Res., Cosmopoliteness of Innovative Dairy Farmers: Case 47(2):137-41. Studies. J. Communication Studies, 31(1) April 2013. Meena, H. R. and Singh, Y. P. (2013) Importance of Datt, R., Jha, S. K., Salaria, N. and Thakur, A. K. information and communication technology tools (2013) Communication Behaviour of Farmers among livestock farmers: A review. Scientific J. Pure Pioneering Dairy-based Innovations at the and Applied Sci., 2013(2)2:1-9. Grassroot Level. Indian J. Dairy Sci., 66 (5) : Sept.- Oct. 2013. Meena, H. R., Jini, Doni, Seth, Pankaj and Meena, K. L. (2013). Job Satisfaction among the technical Datt, R., Jha, S. K. and Salaria, N. (2013) Curiosity workers of veterinary research institute. IJSR, 54(2) Scale for Innovative Farmers. J. Community :173-80. Mobilization and Sustainable Develop., 8(1): 165-68. Meena, B. S., Chauhan, Jitendra, Shanthy, T. Rajula Kadian, K. S. (2014) A tool to measure socio- and Singh, B. P. (2014) Adoption gap and its path economic status of dairy farmers. Indian J. Anim. analysis in feeding practices of dairy animals. Sci., 84 (1): 91-95 Indian Res. J. Ext. Edu. 14 (2) :74-77.

Jaisridhar, P., Sankhala, G., Kumar, Senthil and S. Meena, B.S., Srivastava, A.K., Sankhala, Gopal and Sangeetha (2013) Factors Determining Adoption Verma, H.C. (2013) Dairy Pashuon Ki Utpadak Aur of Scientific Dairy Farming with Special Reference Prajnan Kshamta Ka Jila Faizabad Main Adhyayan. to Farmer’s Call Centre. Pakistan J. Agri. Sci., 50(4) Krishaka Shodh Patrika 2 (1) 39 : 32. : 1-5. Murali, K., Elangovan, R. and Kumar, R. Senthil Kolekar, D. V., Meena, H. R. (2013) Accessibility, (2013)Analysis of Societal Value Dynamics (SVD) Efficiency and impact of extension service of Groundwater Utilization in South Taluk of deliveries to Rural milk producers under contract Coimbatore District, Tamil Nadu. India J. Applied dairy system. Anim. Sci. Reporter, 7(2): 67-74. Sci. Res., 9(4): 2455-62. Kolekar, D. V., Meena, H. R. and Bangar, Y.C. Ponnusamy, K, Nayak, Jyoti, Sarkar, Ananta, Arya, (2013) Dairy farmers attitudes and preference MPS and Prusty, Manoranjan (2013) Comparative towards contract dairy farming. The Indian J. Field performance and gender appraisal of random and Vet. 8(4) : 53-57. line planting in paddy(Oryza sativa) cultivation. Kolekar, D. V., Meena, H. R. and Bangar, Y.C. Indian J. Agri. Sci., 83(10) : 99-101 1. (2013) Constraints perceived by the contract Ponnusamy, K. (2013) Impact of public private farmers and integrating firm under contract dairy partnership in agriculture: A review Indian J. Agri. farming. Tamilnadu J. Vety. and Anim. Sci., 9 (2). Sci., 83(8) : 803-08. Kolekar, D. V., Meena, H. R., Sharma, N. K. and Bangar, Y. C. (2013) Economics of milk production Salaria, Nandani, Jha, Sujeet K., Ram Datt, Tak, Ata- under integrated contract dairy farming in Satara Ul-Munim and Wani, Sajad A. (2013) Constraints in district, Maharashtra Indian J. Dairy Sci., 66(2). the Management of the Model Dairy Plant: A Case Study. J. Community Mobilization and Sustainable Kolekar, D. V. Meena, H. R. Bangar, Y.C. ( 2013) Develop., 8(2): 186-89. Analysis of the efficiency of milk production under contract dairy farming. Indian Vet.J., 90 (8) : 105-06. Sridhar, Jai, Sankhala, G., Kadian, K. S., Kumar, S. and Sangeetha, P. (2013) Factors determing Kumar, R. Senthil, Kumar, Ram, Mohammad, Asif adoption of scientific dairy farming with special and Subash, S. (2013) Marketing Behavior of Dairy reference to farmers call centre of Tamil Nadu. Farmers in Tamil Nadu. J. Global Communication, Pakistan J. Agri. Sci., 50(4) : 549-53. 6(1) : 19-23. Subash, S., Kadian, K. S. and Kumar, S.R. (2013) The

Kumar, R. Senthil, Kumar, Ram, Mohammad, Asif, perceived constraints and suggestions on internet 2013-14 report ndri annual Jaisridhar. P. and Jaduon, Y. (2013) Knowledge use by the students of a national institute. J. Global Analysis and Entrepreneurial Traits among Dairy Communication, 6 (1): 40-43. 119 –– Publications ––

Talata R. and Jancy Gupta (2013). Organizational inland drainage zone of Rajasthan. Int. J. Agril. and Climate as Perceived by Veterinary Assistant Statistical Sci., 9 (2) : 657-60. Surgeons of Andhra Pradesh in India. J. Agri. Edu. and Extn., DOI: 10.1080/1389224X. 2013.803988. Southern Regional Station, Bangalore Popular Articles Kolekar, D. V., Singh, Subod Kumar, Seth, P. and Research Papers Meena, H. R. (2013) Contract Dairy Farming: to Devi, M. C. A., Dixit, P. K. and Subash, S. (2013) boost Indian Dairy. Livestock line, 6 (12): 42-46. “Prosperiety through Dairying: An Empirical Case of Woman Dairy Farmer from Karnataka”. Indian Meena, B.S. and Meena, D.K. (2013) Strategies to Dairyman, 116-19. meet out the feed and fodder requirement in deficit areas. Livestock Tech.. 3(6): 24-25. Franklin Magdaline Eljeeva Emerald, Heartwin A. Pushpadass, Menon Rekha Ravindra, Kulkarni, Meena, H. R. (2014) Mobile Phone Application Rao, Jayaraj and Nath, Battula Surendra (2013) in Agricultural Extension. In the compendium Modeling the heat and mass transfer during frying of ICAR sponsored 10 days short course on of gulab jamun. J. Food Processing and Preservation, Empowerment of farming communities through DOI: 10.1111/jfpp.12168. hybrid communication tools. Division of Extension Education, IVRI, Izatnagar-UP. pp : 72-78 Jadhav, P. V., Das, D. N., Chetana, K. R., Tarate, S. B. and Shome, B. R. (2013) Application of PCR Training Manual based technique for detection of common mastitis pathogens in milk samples of HF crossbred cattle. Sankhala, Gopal, Kumar, Rakesh, Meena, H. R. Global J. Biosci. and Biotech., (3): 409-12. and Meena, B. S. (2014) Commercial Dairy Farming during 28 Jan to 2 Feb.2014, Sponsored by ATMA, Heartwin, A. Pushpadass, F. Magdaline Eljeeva Jhunjhunu, Rajasthan, pp: 95. Emerald, Chaturvedi, Bharat and Rao, K. Jayaraj (2013) Moisture sorption behaviour and Book Chapter thermodynamic properties of gulabjamun mix. J. Food Processing and Preservation. doi:10.1111/ Chakravarty, Ritu and Mehta Nidhi. Coping with jfpp.12199. Climate Variability through ITK Practices for Resilience in Livestock Rearing, Book Chapter Heartwin A. Pushpadass, F. Magdaline Eljeeva (Chapter 22) in: Climate Resilient Livestock and Emerald, Rao, K. Jayaraj, Nath, B. Surendra and Production System. (2013). Book ISBN 978-93-5156- Chaturvedi. Bharath (2013) Prediction of shelf- 150-7.Eds. Singh Sohanvir, Upadhyay R.C., Sirohi life of gulabjamun mix using simulation and Smita and Singh A.K. pp : 234-49. mathematical modeling-based on moisture gain. J. Food Processing and Preservation, DOI: 10.1111/ Forage Research and jfpp.12111. Management Centre Kiran Kumar D. S. and Ghosh, Bikash C. (2013) Research Papers Development of ergogenic drink from hydrolysed whey. Indian J. Dairy Sci., 66 (5) : 371 – 81. Kumar, Rakesh (2013) Boosting barley (Hordium vulgare, L.) production through crop technology Kumar, Santosh, Aravindakshan, T., P., Sangeeta, demonstration in transitional plain of inland A., Pagote, C. N. and Jayaraj Rao, K. (2013) drainage zone of Rajasthan (India). Int. J. Agril. Development of mint flavoured yoghurt spread. Sci., 3 (9) : 26-28. Asian J. Dairy Food Res., 32 (1): 19-24.

Kumar, Rakesh and Agarwal, S. K. (2013) Yield and Majumdar, Madhumita and Nath, B. Surendra yield attributes of wheat (Triticum aestivum L.) as (2013) Estimation of sucrose in gulab jamun by influenced by agrispon and fertonic at varying level Seliwanoff’s colorimetric method. Indian J. Dairy of fertility. Int. J. Agril. Sci., 3 (9) : 29-33. Sci., 66 : 101-07. ndri annual report 2013-14 report ndri annual Kumar Rakesh (2013) Evaluation of crop technology Prasad, Babu, Kadian, G. K. S., Kumar, R. Senthil 120 demonstration of mustard in transitional Plain of and Subash, S. (2013) Use of ICT-Enable Services –– Publications ––

for Empowerment of Dairy Farmers in Andhra Varalakshmi S., and Balasubramanyam, B.V. (2013). Pradesh. J. Global Communication. 6(2). Lactic acid bacteria – A boon to Biopreservation of Foods. Beverage and Food World, 40 : 46-48. Rachana, C. R, Nath, B. Surendra, Reshma, M. V. and Armughan, C. (2013) Variation in grainy Varalakshmi S., Balasubramanyam, B. V. and Nath, texture of commercial ghee in relation to laboratory B. Surendra (2014) Novel strains of lactic acid ghee and its blends. International J. Dairy Tech., 66 bacteria to increase the safety of fermented dairy : 90 -97 foods. Indian Dairyman, 147-48.

Rao, T. V. L. N., Ramesha, K. P., Barani, A., Chauhan, Veena N. and Nath, B. Surendra (2013) β-Glucan as S. S. and Basavaraju, M. (2013) Association of a functional ingredient in dairy foods – A Review. GSTP1 gene polymorphisms with performance Indian J. Dairy Sci., 66 : 461-68. traits in Deoni cattle. African J. Biotech., 12 (24) : 3768-73. Popular Articles

Rekha Menon Ravindra, Rao, K. J., Nath, B. S., Bandla, Srinivas (2013) Producer, processor and Ram, C. (2014) Extended shelf life flavoured dairy consumer triangulation in the growth of Indian drink using dissolved carbon dioxide. J. Food Sci. dairying for socioeconomic and trade prospects. and Tech., 51(1):130–35. 42nd Dairy Industry Conference on “Growth in Indian dairying & trade issues”. 12th to 14th Saravanan, B. C., Das, S., Siju S. J., Tewari, A. K., December 2013, Chennai. Sankar, M., Kataktalware, M. A. and Ramesha K. P. (2013) Babesia bigemina infection in yak Ghosh, Bikash C. (2014) Sorpuriya and Sorbhaja (Poephagus grunniens L.): Molecular detection and – milk sweet delicacies of West Bengal. Indian characterization. Vety. Parasitological: 194 : 58-64. Dairyman, 66 (2) : 68-71.

Shahid, A. S., Ramesha, K. P. Aarif, Ovais and Magdaline Eljeeva Emerald, F., Jayaraj Rao, K., Mir, Nazir Ahmad (2014) Genetic Polymorphisms Heartwin A. Pushpadass, Ghosh, B.C. and Kumar, within Exon 8, 9 and 10 of Heat Shock Protein A. 2013. Chhana podo-A traditional baked dairy (HSP)90 AA in Deoni cattle. Anim. Sci. Reporter, 1 product. Kisan World, 40(9): 33-34. 8 (1) 26-30.

Sivaram, M. and Nayana, K.G. (2013) Hedonic Pagote, C. N. and Jayaraj Rao, K. (2013) Khoa Price Analysis of Teak Logs. Small-scale Forestry jalebi – a new product for Indian dairy industry. . (U.K) 12: 597-609. Beverage and Food World, 40 (2): 57-58, 60.

Subash S., Kadian, K. S. and Kumar, R. Senthil Pagote, C. N. and Jayaraj Rao, K. (2014) Tokir – a (2013) The Perceived Constraints and Suggestions potential food ingredient. Beverage and Food World, on Internet Use by Students of a National Institute. 41 (2): 39-41. J. Global Communication, 6 (1). Ramesha, K. P. and Jayaraj Rao K. (2013) New Surendra Nath, B. and Kulkarni, Satish (2013) Ghee trends in Indian Dairy Sector. Comprehensive – Product with 30% MUFA and numerous nutritive Commodity Intelligence, 13(12): 23-26. properties, Food & Beverage News, 5 : 17 & 19. Ramesha, K.P. and Divya, P. (2014) Recent Varalakshmi S., Balasubramanyam, B.V., Nath, advances in animal genetics for enhancing dairy B. Surendra, Bagath, M. and Rajendran, D. (2014) animal productivity in India. Indian Dairyman. Jan. Use of novel lactic acid bacterial strains with 2014. 94-100. antagonistic activity for the preparation of safe indigenous fermented dairy foods (dahi and raita). J. Food Safety. doi: 10.1111/jfs.12091, 34 : 26–33. Subash, S., Devi, M. C. A. and Dixit, P. K. (2013) “Dairy Extension Services: Critical Issues and Varalakshmi S. and Kirubaharan J. John (2014) Strategies”. Indian Dairyman, 66(1). pp : 174-77.

Effect of Cyclophosphamide on the immune status 2013-14 report ndri annual of chickens vaccinated with Newcastle disease Subash S., Surendranath, B., Blasubramaniam, B. vaccine. Res. J. Biotech., 9 : 53 - 56. V. and Kulkarni, Satish (2013) “Human resource 121 –– Publications ––

Requirements for future Growth in Dairy Sector”. Kumar, Manoj, Dutta, T. K., Singh, Gitam and Indian Dairyman, 140-141. Chaturvedi, Indu (2013) Effect of Lactobacilli culture on the performance of pre-weaned Barbari kids. Swapna, K. S., Jayaraj Rao, K. and Pagote, C. N. Indian Res. J. Genet. & Biotech., 5(4): 278-86. (2013) Yak milk and its products. Indian Dairyman, 65 (12): 72-75. Mamta, Dutta. T. K. and Tripathi M. K. (2014) Effect of levels of concentrate on rumen fermentation and Vyawahare, A., Jayaraj Rao, K. and Pagote, C. methane production in vitro and in weaner barbari N. (2013) Computer vision system for colour kids. Ind. J. Small Ruminants, 20 (1): 24-30. measurement – fundamentals and applications in Mandal, A., Tomar, S. S., Roy, R. and Rout, P. food industry – a review. Research and Reviews: J. K. (2013) Genetic studies of growth traits in Food and Dairy Technol., 1 (2): 22-31. Jamunapari goats of India. Ind. J. Dairy Sci., 66(3): 130-33. Eastern Regional Station, Kalyani Mandal, A., Roy, P. K., Ghosh, M. K., Chatterjee, A. and Das, S. K. (2013) Genetic and environmental Research Papers effects on first lactation traits of Jersey crossbred cattle in an organised herd of Eastern India. Ind. J. Baruah, K. K., Dhali, A., Mech, A., Bora, B., Das, Dairy Sci., 66 (2): 130-33. J., Bora, R., Mondal, M., Sarmah, B. C., Deka, B. C., Rajkhowa, C. (2013) Effect of concentration Mondal M., Baruah, K. K., Chatterjee, A. and and addition method of glycerol on the quality of Ghosh, M. K. (2013) Characterization and Gene cryopreserved mithun (Bos frontalis) spermatozoa. Expression Profiling of Epididymal Sperm J. Anim. Physiol. Anim. Nutr. (Berl)., 97 (6) : 1051-8. Collected from Dead Mithun (Bos Frontalis) Biswas, J., Chakraborty, A., Saha, K., Das, U. S., Pal, Bulls and its Preservation.. Ind. J. Biotechnol. M., Pathak, P., Misra, S. K. and Mandal, A. (2013) Bioengineering Res., 4 (6): 535-42. Flow cytometric sorting of Sahiwal bulls semen Mehra, S., Rana, R., Kumar, V. and Mandal, A. coupled with artificial insemination at organised (2013) Evaluation of combined adjuvant vis-à-vis herd. Ind. J. Anim. Sci, 83 (12): 1275-78. single adjuvant for development of caprine pleura Chatterjee A., Sharma, Puneet, Ghosh, M. K., pneumonia vaccine in goats using indigenous Mandal, M. and Roy, P. K. (2013) Utilization Mycoplasma mycoides subsp. capri isolate. Ind. J. of Azolla Microphylla as Feed supplement for Anim. Sci., 83(5) : 473-76. crossbred cattle. Int. J. Agri. and Food Sci. Technol., 4(3): 207-14. Santra, A., Mandal, A., Konar, S., Banerjee, A., Chatterjee, A., Das, S. K. and Ghosh, M. K. (2013) Chaturvedi, I., Singh, P. K. and Dutta, T. K. (2013) Influence of graded replacement of paddy straw Effect of Herbal Feed on Goat Haematological and with brewer’s spent grain on rumen protozoa, Biochemical Profile.Int. J. Biotech. and Bioengineering enzyme profile and feed fermentation under in Res., 4(3): 257-62. vitro. Ind. J. Anim. Sci., 83 (8) : 806-10.

Chaudhary, U. B., Tripathi, M. K., Gupta, Santra, A., Banerjee, A. and Das, S. K. (2013) Effect Bhavna, Dutta, T. K. and Sirohi, H. V. (2013) of vegetable oils on ciliate protozoa, methane yield, Effect of inorganic and organic zinc and copper enzyme profile and rumen fermentation in vitro. supplementation on performance, Nutrient Anim. Nutr. Feed Technol., 13: 181-93. utilization, rumen fermentation and blood biochemistry of kids. Indian J. Anim. Sci., 83 (12): Santra, A., Konar, S., Banerjee, A. and Das, S. 1313–22. K. (2013) Effect of betel (Piper betle) leaves supplementation on nutrient utilization, milk yield Karunakaran, M., Devanathan, T. G., Jawahar, and its quality in lactating crossbred cows. Anim. Tilak Pon, Manimaran, K. Chitra, Anand, Dhali, Nutr. Feed Technol., 13: 281-90. A. and Selvaraju, S. (2013) Effect of heparin binding

ndri annual report 2013-14 report ndri annual proteins on the in vitro sperm characters and lipid Sharma, A. K., Mahapatra, Sushil. K., Mohanty, peroxidation status of frozen thawed bull semen. A. K. and Das, S. K. (2013) Purification of heparin 122 Ind. J. Anim. Sci., 83 (8): 788–90. binding oviduct specific proteins and its effect on –– Publications ––

in vitro embryo development in cattle. Ind. J. Expt. Chatterjee, A., Ghosh, M. K., Mandal, A., Das, S. K., Biol., 51 (5) : 347-51. Dutta, T. K., Roy, P. K., Santra, A., Singh, L. (2013) Nutritional Interventions for Sustainable Dairy Sharma, D. K. and Mandal, A. (2013) Factors Production in Eastern India. In: Dairying in Eastern affecting gastrointestinal parasite infections in India: Issues and Challenges., Edited by A. Mandal, goats in semi-arid rural ecosystems in India. Vety. A. Chatterjee, S. Bandyapadhyay, P. Dandapat, Sci. Develop., 3 : 5, 24-27. M.K. Ghosh, S. K. Das and T. K. Dutta, ERS-NDRI, Kalyani, Nadia, West Bengal, pp: 27-40. Popular Articles Chatterjee, A., Bhakat, C., Garai, S., Mondal, M., Mandal, Ajoy, Chatterjee, A., Ghosh, M. K., Das, Karunakaran, M. and Ghosh, M. K. (2014) Scientific S. K. and Dutta, T. K. (2013) Crossbreeding of Management of Calves. Scientific Dairy Farming cattle in India- its impact and issues. The North- Practices Ed: S. Garai, A. Chatterjee, A. Mandal East Veterinarian, Vol. XIII, No. 1. Apr-June, 2013, and M. Karunakaran . ERS of NDRI, Kalyani, West pp: 29-32. Bengal, pp: 31-34. Mandal, Ajoy and Dutta, T. K. (2013) Jakhrana-a Chatterjee, A., Goswami, A., Garai, S., Ghosh, M. threatened goat breed of India. Raksha Technical Review, Vol. III, Issue 2, June, 2013, pp. 43-46. K. and Bhakat, C. (2014) Backyard Azolla Production for feeding of Livestock. Scientific Dairy Farming Roy, P. K., Mandal, Ajoy, Chatterjee, A. and Ghosh, Practices Ed: S. Garai, A. Chatterjee, A. Mandal M. K. (2013) Good udder health and its significance and M. Karunakaran . ERS of NDRI, Kalyani, West in milk production of cattle. Raksha Technical Bengal, pp: 53-59 Review, Vol. III, Issue 4, December, 2013, pp. 19-22. Chatterjee, A., Ghosh, M. K., Mondal, M. and Bhakat, Champak (2013) Scope for Improvement Review Articles of Animal productivity though Efficient Utilization Das, S. K. (2011) Verification and its role in of Non-Conventional Feed Resources Advance reproduction. Livestock International. 15(4):10-11. in Livestock Production Management Technologies organised by Eastern Regional station, Indian Mandal A., Das. S. K., Karunakaran, M., Bhakat, Veterinary Research Institute, Belgachia, Kolkata, C. and Dutta. T. K. (2013). Application of genomic from Nov., 12 – Nov. 25, 2013, pp: 22-31. selection in livestock improvement. Res.and Rev.: J. Dairy Sci. and Technol., 2(3): 5-11. Das, S. K., Mandal, A., Chatterjee, A., Ghosh, M. K., Malakar, D., Mohanty, A. K., Dutta, T. K. Mondal, M., Baruah, K. K. and Rajkhowa, C. (2014) (2013) Reproductive Biotechniques in Livestock. Mithun: An Animal of Indian Pride. Livestock In: Dairying in Eastern India: Issues and Challenges., Research for Rural Development. Volume 26, Article A. Mandal, A. Chatterjee, S. Bandyapadhyay, #6. Retrieved April 1, 2014, from http://www. P. Dandapat, M.K. Ghosh, S. K. Das and T. K. lrrd.org/lrrd26/1/mond26006.html Dutta, ERS-NDRI, Kalyani, Nadia, West Bengal, pp: 51-63. Book Dutta, T. K., Chatterjee, A., Mandal, A., Ghosh, M. Mandal, A. Chatterjee, A. Bandyapadhyay, S., K., Das, S. K. (2013) Relevance of Micronutrients Dandapat, P., Ghosh, M. K., Das, S. K. and Dutta, T. K. (2013). Dairying in Eastern India: Issues and in Reproduction of Ruminants in Eastern India. Challenges, ERS-NDRI, Kalyani, Nadia, West In: Dairying in Eastern India: Issues and Challenges., Bengal. A. Mandal, A. Chatterjee, S. Bandyapadhyay, P. Dandapat, M.K. Ghosh, S. K. Das and T. K. Dutta, ERS-NDRI, Kalyani, Nadia, West Bengal, pp: 64-74. Book Chapters

Bhakat, C., Mandal, A., Karunakaran, M., Garai, Karunakaran, M., Chakurkar, E. B., Ratnakaran, S., Chatterjee, A. and Ghosh, M. K. (2014) U., Naik, P. K., Singh, N. P. (2013) Infertility Management of Dairy Animals for better fertility. problem in dairy cows. Compendium of ICAR

Scientific Dairy Farming Practices Ed: S. Garai, A. sponsored short course on IFS conducted during 2013-14 report ndri annual Chatterjee, A. Mandal and M. Karunakaran . ERS 4th - 13th June, 2013 at ICAR Research Complex for of NDRI, Kalyani, West Bengal, pp: 28-30. Goa, Old Goa. 123 –– Publications ––

Karunakaran, M. and Mondal, Mohan (2013) cattle Improvement in Eastern India. In: Dairying Sexually transmissible diseases in farm animals. in Eastern India: Issues and Challenges., Ed: by A. Compendium of Short Term Training Course on Mandal, A. Chatterjee, S. Bandyapadhyay, P. Laboratory Diagnosis of Animal Diseases and Dandapat, M.K. Ghosh, S. K. Das and T. K. Dutta, Zoonoses. From 12th – 25th September, 2013 Eastern published by the Director, NDRI at ERS-NDRI, Regional Station, Indian Veterinary Research Kalyani, Nadia, West Bengal, pp: 41-50. Institute, 37, Belgachia Road, Kolkata – 700 037. pp: 141-147. Mandal, A., Chatterjee, A., Karunakaran, M., Das, A. S. K., Ghosh, M. K. and Dutta, T. K. (2013) Karunakaran, M., Mondal, Mohan, Mandal, Management of infertility of cows: pros and cons. Ajoy and Dutta, T. K. (2013) Advances in Semen Scientific Dairy Farming Practices ERS of NDRI, Evaluation-Molecular Indicators of Bull Fertility. Kalyani, West Bengal, pp: 23-27. In: Advances in Livestock Production Management Technologies. pp: 37-50 Eastern Regional Station, Mondal, M., Baruah, K. K., Chatterjee, A., Ghosh, Indian Veterinary Research Institute 37, Belgachia M. K., Rajkhowa, C. (2013) Global gene expression Road, Kolkata - 700 037, during 12-25 November, in developing longissimus muscle from two different bos species. In: Compendium of First Mandal, M. Karunakaran, Das, S. K., Ghosh, M. K. International and Third National Conference on and Dutta, T. K. (2013) Breeding policies for dairy Biotechnology, Bioinformatics and Bioengineering cattle and buffaloes under different agro-climatic held at Tirupati, Andhra Pradesh during June 28- region of India. In: Advance in Livestock Production 29, 2013, pp: 50. Management Technologies” organised by Eastern Regional station, Indian Veterinary Research Mondal, M., Karunakaran, M., Chatterjee, A., Institute, Belgachia, Kolkata, from Nov., 12 – Nov. Bhakat, C., Das, S. K. and Ghosh, M. K. (2014) 25, pp: 22-31. Physiology of Female Reproductive System of the cow. Scientific Dairy Farming Practices Ed: S. Garai, Mandal, A., Chatterjee, A., Das, S. K., Ghosh, M. A. Chatterjee, A. Mandal and M. Karunakaran . K., Dutta, T. K. (2013) Scope and Challenges of ERS of NDRI, Kalyani, West Bengal, pp: 11-20.

Dr. N. N. Dastur Memorial Oration Award to Dr. V. M. Katoch, Director General, ICMR

Dr. K. K. Iya Memorial Oration Award to Dr. S. K. Bandyopadhyay, Member, Agricultural Scientist Recruitment Board ndri annual report 2013-14 report ndri annual

124

9 The followingtechnologieswerecommercialized Transfer ofTechnology and generatedatotalrevenueof` 184organizations providedservicesto Institute real needsofdairyindustry.During2013-14,the the fulfill to efforts research their fine-tune and such interactions helped the scientists to focus and industry.Feedbackfromindustrythrough for two-waycommunicationbetweenscientists Consultancy servicesalsoprovidedaviamedia scientists rendering such service as an incentive. the wassharedamong money ofthe also part the Institute’sinfrastructureand R&D work and consultancy serviceswasutilizedforstrengthening different organizations.Revenuegeneratedfrom from assistance loans/financial secure to them whichenabled projectreports, providing by helped manyprospectivedairyentrepreneurs In year2013-14,theConsultancyServices of NDRI on 6 industries inNDRI–IndustryMeetorganized from officials before presented were technologies dairy equipments,mineralmixtures,etc.These products, milk fortified foods, health safety, milk of technologiesthathaverelevanceinmilkquality, The scientistsatNDRIhavedevelopedanumber NDRI IndustryMeet whey jaljeera mix, bajralassi, butter melter, butter bajralassi, jaljeera mix, whey antibiotic detectioninmilk,whey-mango beverage, test, striptestfor neutralizers, strip testfor urea, interest intechnologies of detergent detection No. S. 1. 7. 6. 5. 4. 3. 2. COMMERCIALISATION OFTECHNOLOGIES CONSULTANCY, PATENTS& th January,2014.The industries have shown Commercialized Title ofTechnology Enterococci inmilk micro-technique fordetectionof A novel selective medium and detection ofdetergentinmilk New colourbasedtestforrapid Cation &anionmineralmixture Cation &anionmineralmixture Cation &anionmineralmixture Iron fortifiedbiscuits milk for detectionofL.monocytogenesin Two stageenzymebasedassay” I ndustrial 1, 54,42,239/-. 5 Signed) Licensing Agreements Mode ofTransfer(MoU/ 19 12 15 15 5 5 th th th th th th th July,2013 July,2013 July,2013 March,2014 February,2014 July,2013 July,2013 C onsultancy for submissiontopatentoffice. before thesearebeingrecommended applicability ITMC meetings for their noveltyand commercial 2014. Patentapplicationsareexaminedduring were heldon1 During theyear2013-14,fiveITMCmeetings pricing of technology is also decided byITMC. inventors tosubmitpatentapplications.The commercialization, etc. ITMC/ITMU encourages related tointellectualpropertymanagementand institute levelfortheallmandatorydecisions ITMC ishighestdecisionmakingbodyatthe (ITMC) Institute TechnologyManagementCommittee G.R. Patil,JointDirectorinaugurated themeet. New DelhiandSachdevaMilk Dairy, Sangrur.Dr. Pardesh; MART, Noida; GreenAgri Miles Pvt.Ltd., Kamdehnu HitkariManch, Bilaspur,Himachal Delhi; HetaudaDairyIndustries (P)Ltd.Nepal; Dairy FederationLtd, Jaipur; Delhi Milk Scheme, participation was from Rajasthan Co-operative in adaptionoftest by dairy industry. The industrial in milk were given toimpress upon the simplicity and striptest fordetectionofneutralizersandurea mixtures. Thelivedemonstrationsofdetergenttest andmineral machine, feedblocks making ghee July, 2013,27 S Purchaser ofTechnology Federation Ltd.,Jaipur Rajasthan Co-operativeDairy Meerut U.P. Feeds(P)Ltd., Livestock Shakti Jaipur, Rajasthan Shree JeeFeedSupplements, U.P. Kamdhenu Feeds,Saharanpur, M.P. Shree Shivani Agro. Indore, Pvt. Ltd.Hyderabad (India) Diagnostics Neugen Pvt. Ltd.Hyderabad (India) Diagnostics Neugen ervices th September,2013and 15 st April, 2013,7 th June2013,25 License Fee ` 6,00,000 ` 1,00,000 ` 25,000 ` 25,000 ` 55,000 ` 3,50,000 ` 7,50,000 th February, th

125 ndri annual report 2013-14 126 ndri annual report 2013-14 5. 4. 3. 2. 1. Patent ApplicationsSubmittedtoOffice –– Consultancy, Patents &Commercialisation of Technologies ––

DEL/2013) (BCM7). (PatentApplicationNo.3703/ Aptamer specificforBetacasomorphin7 No. 3472/DEL/2013) and processforthesame.(PatentApplication ofaddedureainmilk detection based A strip No. 2778/DEL/2013) antiresorptive activity.(PatentApplication A peptidewithosteoanabolicand (Patent ApplicationNo.1864/DEL/2013) B1. and M1 aflatoxin for specific Aptamers (Patent ApplicationNo.1357/DEL/2013) for detection of A novelenzymesubstratebasedrapidassay NDRI IndustryMeet 6 th organised on January,2014 L. monocytogenes in milk.

• • • • • • the ExtensionDivisionofNDRI The followingwereassessed and transferredby 3. 2. 1. List ofPatentgranted

Value addeddairybased foodproducts Scientific udder health management practices Information system on clean milk production Scientific calfrearingpractices High yielding location specific fodder crops Timely heatdetectionindairyanimals 22.11.2013) cow and buffalo milk. (Patent No. 257958, A PCRbasedmethodofdifferentiating 01.11.2013) cholesterol ghee.(Patent No.257783, Lab scale process for preparation of low No. 257068,30-08-2013) concentrate. base/ flavour cheddar Process formanufactureofspraydried Transfer ofMOUbetween RCDF andDr.G.R.Patil, Sh. PritamSingh-MD, Rajasthan representing Development, Govt.of Joint Director(Acad) RCDF andPrincipal representing NDRI NDRI andRCDF: Secretary, Dairy

(Patent

10 SRS, Bangaloreand23 projects of NDRI was taken upduring5 ofNDRIwastaken projects locational projects.Themid-termreviewofIRC ofmulti-disciplinary/multi- inrespect Institute ofthe groups/divisions the between linkages research proposalsandtoadviseonfosteringof completed researchprojects,toconsiderthenew (IRC) aretocriticallyreviewtheon-goingand The keyfunctionsofInstituteResearchCommittee Institute ResearchCommittee(IRC) NDRI Karnal. chairmanship ofDr.B.N.Mathur,FormerDirector, work in front line areas such as cloning, stem by scientistsandstudents in pursuingresearch this programme. The facilities arebeingused high costequipmentshave been procured under number of laboratories have been renovated and Technology, dairyeducationwillalsogetboost.A award ofNAIPprojecton‘e-coursesDairy is on basic and strategic research, but with the Although majorthrustofNAIPprojectsatNDRI is eitheraleadcentreorconsortiumpartner. There arenineteenNAIPprojects;inwhichNDRI National Agricultural Innovation Project (NAIP) Director (Res.),NDRIKarnal. A. K.Srivastava,Director/orDr.G.R.PatilJoint respectively. IRCmeetingswerechairedbyDr. Karnal washeldon4 the Institute. The meeting of theRAC of NDRI, see that these are consistent with themandate of and to the researchachievementsofInstitute global contextinthethrustareas and ii)toreview research programmes based onnationaland The mainfunctionsoftheRACarei)tosuggest Research AdvisoryCommittee(RAC) by R&Dactivities. visualized toachievethemandateofInstitute the Institutearemajor functions existingand documentation byundertakingpublicationsof database andtechnologybankresearch external mechanisms;developmentofresearch levels; researchmonitoringthroughinternaland research organizations at national and international development; establishmentoflinkageswith of researchendeavoursandinfrastructure managing researchfunctions.Strategicplanning approach forof professionalmanagement in recent years has necessitated introduction Karnal NDRI at activities R&D of Intensification August, 2013atNDRI,Karnal,12 EVALUATION (PME) RESEARCH PRIORITIZATION,MONITORINGAND th rd February, 2013 underthe February, July,2013ERS,Kalyani, th August,2013 th - 8 th

women andothergenderrelated issues;Success welfare,the development and empowerment of by theInstitutefor the Institute;Initiativestaken achievements andtechnologies developedby significant the highlighting by NDRI on reports preparedvariousother 2013-2014.Theunit Report of NDRI for inclusion inICAR/DARE Annual also prepared reports on research achievements also broughtoutduringthisperiod.Theunit News” -aquarterlynewsletterinEnglishwere Committee forNDRI,Karnal.Fourissuesof“NDRI Advisory Research by identified Programmmes were also documented under the Research through thiscell.ResearchProjectsoftheInstitute Report 2012-2013waseditedandpublished repute. Duringtheperiod under report,Annual various journals ofNationaland International Bulletins, etc.preparedattheInstituteandthrough Reports, MonthlyTechnicalReports/ Annual Reports,SixMonthlyQuarterly and disseminationofresearchoutputthrough of the Instituteis responsible for documentation The Research Documentation and Information Cell Research DocumentationandInformation and sixmonthlyreportssubmittedtoICAR. targets fixed for the next six months was updated and achievementsoftheprecedingsixmonths database ofresearchprojectscontainingthetargets the projects in operationduringtheyear2013.The A databaseofresearchprojectswasupdatedforall Database Management at NDRIforsmoothfunctioningofPIMSactivity. of alltheon-goingandcompleted researchprojects project atIASRI,NewDelhiandPIs PI ofthe with acted asanodalagencytofacilitateandcoordinate introduced incollaborationwithIASRI. Theunit computerization ofresearch projects under PIMS The PME Unit implemented on-line database/ Project InformationManagementSystem(PIMS) and CSIRlaboratories. opportunity toNDRIdeveloplinkageswithIITs scientist externaltoICARsystem.Ithasprovided to monitor progress. CAC is headedbyrenowned problems (ii)to provide technical guidance and (iii) were heldregularlyto(i)addressadministrative Consortium Advisory Committee (CAC) meetings Consortium Implementation Committee (CIC)and genomics. cell, nanotechnology,biosensor,proteomicsand 127 ndri annual report 2013-14 128 ndri annual report 2013-14 achievements (last 5 years) of the Institute. The unit achievements (last 5years) oftheInstitute. The unit commercialized; Preparedconcisereportonthe on technologiesdeveloped,transferredand under RighttoInformationAct,2005; organizations; IPR issues; Provided information of NDRI;Establishmentlinkageswithvarious to theInstitute;Institutionalperformanceinrespect Conference (2013)coveringrelevantpointsrelating regarding tentativeagendaitemsforDirector’s Newsletter. The unitprepared theinformation ARIS Newsletter, ICAR;IAUA Newsletter; VCI onNDRIforinclusion inof ICAR;Information meetings forpublicationinDirectoryofEvents important conferences,seminars,workshopsand Agriculture. Theunitalsopreparedinformationon made by the Parliamentary Standing Committee on of theInstitutewithrespecttorecommendations six months.Theunitalsopreparedcomments of next of theresearchachievementswithtargets Reports Progress Monthly Six events; significant month; MonthlyProgressReportsconsisting of thenext purchase andcivilworkstargets finance, research, of achievements the containing area of animal sciences; QuarterlyProgressReports and dairy industry;Transfer of technology inthe research technologiesforthefarmingcommunity extension activities of theInstitute; Development of Stories ofprogressivefarmersbasedonthe ‘Swarn’ maleclonedcalfwiththeteamofscientists that producedit

Symposia/Workshops. processed forpresentationatvariousSeminars/ repute. Inaddition,abstractsofpaperswerealso in variousjournalsofNationalandInternational scientists wereprocessed bythe unitfor publication questionnaires. Research papers submitted bythe sending thedesired information throughseveral by ordinated withotherOrganisations/Institutes Audit (external)queriesfromtimetoandco- prepared repliestoParliamentquestionsand National AgriculturalResearch System. This unit Agent for Management of Change intheIndian with theobjectivetomakeICAR as the Catalyst Monitoring andEvaluationSystemforNAIP,ICAR for submissiontoNationalDirectorNAIP unit also prepared and collated information The fields. allied or chain supply development, harvest technologies,packaging,newproducts at NDRI in the areas of food processing. Post on researchprojectsundertaken/undergoing ICAR;ConsolidatedinformationCommittee Director’s Conference/BOMofNDRI/Regional (AS) atRegionalStations/GoverningBody/ during themeetingsofQRT/RAC/VisitDDG action takenreportsonrecommendations emerged EFC memodocumentoftheInstitute;Prepared initiated theexercise for formulation of XII plan prepared reportonmid-termreviewofXIplan; Sh. ShashankAnand,Supdt.ofPolice,Karnallaunching iron andvitaminfortifiedmilkattheparlour –– Research Prioritization,Monitoring and Evalution ––

11 Financy)” atPhillipinesfrom8 the areaof“CarbonStockAssessment (Carbon Division, NDRI, Karnal attended the trainingin Dr. Sohanvir Singh, PrincipalScientist, DCP Center, Indianapolis, Indiana, USA from 8 of Animal Science(ASAS),IndianaConvention Science Association (ADSA) and American Society Joint AnnualMeeting2013oftheAmerican Dairy Division participatedandpresented apaperat Dr. BimleshMann,PrincipalScientist,DC Identification of Rumen Fungi” at U.K. from 11 from U.K. at Fungi” Rumen of Identification on “Development ofMolecular Markers for Microbiology Division was deputed fortraining 2013. Dr. A. K. Srivastava, in Foods(CCRVDS)atUSAfrom26 Codex Committee on Residue of Veterinary Drugs 31 May to2 Lanzhou University,Lanzhou,Chinafrom27 for DevelopingCountries(SAADC2013)”heldat Conference on“Sustainable Animal Agriculture presented leadtalkintheFourthInternational Dr. S. K. Sirohi Principal Scientist, DCN Division July, 2013. Division wasdeputedtoattend21 Dr. NareshKumar,PrincipalScientist,DM 20 University of Wisconsin-Madison (UW), USA from Programme Technology Transfer Course at NDRI, KarnalwasdeputedtoattendKhurama Dr. RajanSharma,Senior Scientist, DCDivision, Countries” at Sri Lanka from 5 Conservation and Management in SAARC “Farm AnimalGeneticsResources Evaluation deputed toattendtheconsultation meeting on Dr. A.K.Srivastava,Director,NDRI,Karnalwas 2013. 2013. participated inthe6 Mr. Pravin Sawale, Ph.D.(DairyTechnology) Kingdom w.e.f.26 Conference at The University of Sheffield, United Review Meeting atEthiopia during 20 participated inCGIARLivestockandFishAnnual Dr. A.K.Puniya,PrincipalScientist,Dairy th st July,2013. Julyto8 HUMAN RESOURCEDEVELOPMENT nd November,2013. th August,2013. S th cientists -28 th InternationalGranulation th Director June,2013. th th /O - 15 - 6

NDRI, Karnal th th -30 fficers th July,2013. st th September, sessionof -22 th August, August, nd th May, May, - 12 /S th - th th

cholars 10 Dr. DheerSingh,PS,ABC Division participated Research Projectfrom1 (DST-DAAD)JointWorkshop onIndoGerman the attend Dheer Singh,PS,ABCDivisionto Dr. Livestock ValueChain”ACCRG,Ghanafrom5 conference on“Mainstreaming in the participated Dr. B.S.Chandel,PS,DESMDivision,NDRI Congress during6 participated intheWorkshop on“Reproductive Dr. Mohan Mandal, Sr. Scientist, ERS, Kalyani 6 8 Commission in Food Science and Nutrition” from Koethen, Germany on “European Programme Programme at Hochsscule, Anhalf (FH) in Karnal availedEraasumuMundusScholarship Dr. R.K.Malik,Head,DMDivision,NDRI November, 2013. Immunologist held at Seoul, Korea from 7 Fall ConferenceoftheKoreanAssociation three ProbioticLactobacilliStrains”inthe2013 Evaluation ofImmunomodulatoryPotential for presentingposterentitled,“Comparative Biochemistry Division,got“TravelGrantAward” Dr. SumanKapila,PrincipalScientist,Animal Mr. TanedjeuSonfackKemgangstudentof Isalambad, Pakistanfrom23 and Agriculture Challenges and Opportunities” at participated intheRegionalWorkshopon“Youth Dr. A.K.Srivastava,Director,NDRI,Karnal Bangaldesh from16 Preservation andTransportation”atDhaka, Buffaloes SemenCollection,QualityAssurance, in thesixth meetingon“HighYielding during Dr. A.K. Chakravarty, PS& I/CABRC participated 23 Nutritional ConferenceatLahore,Pakistanfrom participated intheInternational Livestock Dr. A.K.Tyagi,PS,DCNDivision,NDRI from 16 Food Science Technology & Nutrition atBelgium NDRI, availedErasmus Mundus Scholarship in DCNDivision, PrincipalScientist, A.K.Tyagi, Dr. th th th rd November,2013. –21 WorldBuffaloCongressand7 -24 st th th May,2013. T October,2013. - 30 rained th September,2013. th –8 th A -17 th st broad May,2013atThailand. –30 th November,2013. rd th -24 June2013. th th October,2013. AsianBuffalo th – 8 th - th

129 ndri annual report 2013-14 130 ndri annual report 2013-14 SARDI, Australiafrom11 Biotechnology forAgriculturalResearch”at –– HumanResource Development –– University ofHongKong,on17 India: The WhiteGold for Nutritional Security”at in was invitedtodeliveralectureon“Dairying Dr. A.K.Srivastava,Director,NDRI,Karnal invited to speak at the Riddit Institutes Agrifood Riddit Institutes the invited tospeakat Dr. A.K.Srivastava,Director,NDRI,Karnalwas Hong Kong. at SchoolofBiologicalSciencestheUniversity Dr. O.K.Hooda,PS Mrs. Vimala,Sci. Mrs. M.K.Vedavathi,Sci. Dr. K.JayarajRao,PS Dr. B.SurendraNath,PS Dr. GautamKaul,PS Dr.(Mrs) BimleshMann,PS Dr. M.L.Kamboj,PS Dr. R.K.Baithalu,PS Dr. T.K.Mohanty,PS Dr. PawanSingh,PS Dr. M.S.Chauhan,PS Name &Designation Dr. NareshKumar,Sr.Sci. Dr. D.Malakar,PS Dr. A.K.Mohanty,PS Dr. M.S.Chauhan,PS Dr. H.R.Meena,Sci. Dr. AsifMohammad,Sci. K. Jha,Sr.Sci. Dr. Gopal Sankhala, Sr. Sci. Dr. S. Dr. B.S.Meena,Sr.Sci. Mrs. RituChakravarty,Sr.Sci. Dr. DheerSingh,PS Dr. S.Varalakshmi,PS Dr. S.Subash,PS Dr. M.A.Kataktalware,PS SCIENTISTS/OFFICERS PARTICIPATIONINWORKSHOPS/SEMINARS/ CONFERENCES/TRAININGS WITHININDIA th –14 of India”atCSWRI,Avikanagar. Management inSmall Ruminants in Hotand regions Workshop on“ClimateResillient Shelter&Stress Dairy ScienceCollege,Bangalore. Backdrop ofFSSAIAct2006”at Food Standardsinthe National Seminaron“ChangingScenarioofDairyand NASC NewDelhi. Nanotechnology” inBrainstormingWorkshopof Presented apaperentitled“Applicationof at FICCI,NewDelhi. National Conference on “Animal Disaster Management” Production” atNewDelhiorganizedbyIMV. Workshop on“AdvancementinSemen–analysisand Animals inBiotechnology”atJharnapani,Nagaland Delivered lectureon“StemCellandCloninginFarm Title ofWorkshop/Seminar/ConferencesTraining 2013) atHyderabad. Conference (NIABIIncubation Indian Agri-business “Emerging Trends in Biotechnology” at IVRI Izatnagar. Delivered lectureintheNationalSymposiumon PG collage,BakshiKaTalab,Lucknow,UP. Livelihood”, held atChandraBhanuGuptAgriculture Extension EducationinHolisticDevelopmentofRural National Seminar2013on“SocialDimensionsof Extension andResearch”heldatIARI,NewDelhi. Brain Stormingsessionon“DevelopmentinAgricultural Sciences, Hisar. Biotechnology, LLR University of Veterinary & Animal DBT-HRD ProgramatDepartmentofAnimal IAH&VB, Hebbal,Bangalore. and GovernanceforQualityServicestoFarmers”at Veterinary Research,Education,RegulatoryReforms International Conference on “Thrust Areas in th th February,2014. February, 2014 February, National UniversityofSingapore,Singapore of Medicine,NationalUniversitySingapore Department ofMicrobiology, YongLooLin School collaborative research programme during 19 discuss to North inPalmerston Institute Riddit the Summit inWellington,NewZealandandtovisit and Biotechnology”on25 Singapore SocietyforMicrobiology organized by Workshop on“NewApproachforMicrobialStudy Ms. KiranThakurparticipatedinHandson February, 2014. 2 26 23 17 16 3 Period 26 8 26 26 26 16 th th rd nd -26 th th th th th rd th th th -10 April,2013 May,2013 –27 –27 April,2013 April,2013 April,2013 -18 -17 April,2013 April,2013 th th th th April,2013 April,2013 th th April,2013 September,2013. April,2013 April,2013 th –21 st

Dr. A.Chatterjee,Sr.Sci. Dr. M.Mondal,Sr.Sci. Dr. DheerSingh,PS Dr. P.S.Minz,Sci. Dr. LathaSabikhi,PS Dr. S.K.Tomar,PS Dr. R.K.Malik,Head Dr. RajanSharma,PS Dr. K.Datta,Head Dr. ManiMaran,Sci. Dr. A.Kumaresan,PS Dr. T.K.Mohanty,PS Sh. MithleshKumar,Sr.F&AO Dr.(Mrs.) MadhuMohini,PS Dr. DheerSingh,PS Professor Dr. (Mrs.) Meena Malik, Associate Dr. Raghu,H.V.Sci. Dr. NareshGoel,PS Dr. RajanSharma,PS Dr. MukeshBhagat,Sr.Sci. Dr. A.Manimaran,Sci. Dr. Rubina Kumari Baithalu, Sci. Professor Dr.(Mrs.) MeenaMalik,Associate Dr. S.K.Tomar,PS Dr. S.Subash,PS Dr. Narender Raju Panjagari,Sci. Dr. V.K.Gupta,Head Dr. S.K.Tomar,PS held atTirupati,AndhraPradesh. “Biotechnology, BioinformaticsandBioengineering” First InternationalandThirdNationalConferenceon Science toCommercialization”at Delhi. DBT sponsoredBIRAC-CDSAregulatory meet“From Agriculture &Technology(MPUA&T),Udaipur. Prosperity” heldatMaharanaPratapUniversityof with QualityAssuranceforAugmentingRural National Seminar on “New Vistas in Food Processing Lalit, NewDelhi Organized byConfederation of Indian Industry, Hotel Solutions towardsaSecondWhiteRevolution” India DairySummit,2013on“SustainableandInclusive Station, ofNDRI,Bangalore. Foundation DayCelebrationsatSouthernRegional at NASCComplex,Pusa,NewDelhi. M/s ELANCOsponsoredrBSTStudiesReviewMeeting Area, Phase-II,Noida(UP) Audit Sensitization”atA-52,Sector62,Institutional Training Programmeon“FinancialManagement& Traububg” atIndiaHabitatCentre, NewDelhi Workshop on “IPCC Green House Gas Ubvebtirt Delhi. Biology, AllIndiaInstituteofMedicalSciences,New Reproductive Health”atDepartmentof Symposium on“Impact ofEndocrine Disruptors on Styles andStrategies”atSRMUniversity,Chennai. LearnersLearning Century ELTAI Conferenceon“21st Made OralPresentationat8thInternationaland44th Complex, NewDelhi. in milk” during Agri-Tech Investors Meet atNASC Presented thetechnologyof“Detectiondetergent Management” atNAARM,Hyderabad. Refresher Course on “Agricultural Research University atMadrasVety.College,Chennai. Importance OrganizedbyTamilNaduVety.&AS Emerging &TransboundaryDiseasesofGlobal at CIFA,Bhubaneshwar mandate area ofNDRI as a partof Module of FOCARS Three monthsProfessionalattachmenttrainingona Lucknow. ‘How ELT in theGlobalContextatAmityUniversity Presented apaperat“ELT Conference on ‘Who’, ‘What, 90th yearofestablishmentbySRS,NDRI,Bangalore MANAGE, Hyderabad. Animal HusbandryandFisheriesSectors”heldat Strengthen Strategiesto Workshop on“Extension Evaluation ofPackingMaterial”atMumbai. Training programmeon“TestingandQuality IASRI, NewDelhi. “ICT forCapacityBuildinginAgriculturalEducation” Agricultural EducationDivisionofICARonthetheme Annual Workshop of CAFT Directors, organized by the in Agriculture”heldatNASC,NewDelhi For Basic Strategies & Frontier Application Research Third AnnualReviewWorkshopof“NationalFund 28 30 21 24 1 31 20 29 20 18 18 15 15 17 18 1 20 11 26 22 st st July,2013 July,2013 th th st th st th th th th th th nd th th th th th th –22 May,2013 May to 16 -29 -21 –24 -19 -31 –31 –27 July,2013 July,2013 –20 -19 -27 -16 -12 –– HumanResource Development –– –23 th th th st st th th June,2013 nd th st th th th May,2013 July,2013 rd June,2013 July,2013 July,2013 July,2013 July,2013 May,2013 July,2013 July,2013 June,2013 July,2013 July,2013 th August, 2013 131 ndri annual report 2013-14 132 ndri annual report 2013-14 –– HumanResource Development –– Director (Admn.)&Registrar Sh. J. K.Kewalramani, Joint Sh. PradeepBehare,Sci. Dr. I.K.Sawhney,PS Dr. A.K.Singh,Sr.Sci. Dr. M.A.Kataktalware,PS Dr. Narender Raju Panjagari,Sci. Mr. K.L.Sampath,Sci. Mr.. B.R.S.Murthy,Sci. Mr. Chitranayak,Sci. Dr. S.Subash,PS Dr. S.Varalakshmi,PS Dr. M.A.Kataktalware,PS Dr. S.Jeyakumar,PS Dr. M.C.A.Devi,PS Dr. M.Sivaram,PS Dr. B.Srinivas,PS Dr. K.P.Ramesha,PS Dr. P.K.Dixit,PS Dr. B.SurendraNath,PS Dr. S.Kulkarni,Head Dr. D.Malakar,PS Dr. M.Sivaram,Sr.Sci. Dr. P.S.Minz,Sci. Dr. B.C.Ghosh,PS Dr. I.K.Sawhney,PS Dr. A.K.Dodeja,Head Dr. RajanSharma,PS Dr. S.N.Rai,EmeritusScientist Dr.(Mrs.) MadhuMohini,PS Mrs. Vimala,PS Mrs. M.K.Vedavathi,PS Dr. A.K.Chauhan,PS Dr. B.S.Chandel,PS Dr. K.Datta,Head JNU (Old)CampusofPalmsMarg,NewDelhi. Workshop on “RighttoInformation Act, 2005”atISTM, Indian HabitatCentre,NewDelhi. 7th Asian Conference on “Lactic Acid Bacteria” at Hilton, Andheri,Mumbai. Annual ConventionofDairyEngineers-2013atHotel New Delhi. Ashoka,Chankayapuri, Decade…Frame Work”atHotel National Workshopon“SkillingIndiaforNext Karnataka. Kanara, Sirsi,North and SRSofNDRI,Bangaloreat Malnad GiddaFarmers’organizedbyIVRI,Izatnagar and Entrepreneurs Karnataka, of Officers Veterinary Participated intheInterfaceMeetofScientistswith Solution” atJaipur(Rajasthan) of MilkIssues & a talkon“GoodNutrition&Quality National Nutrition Week one day seminar and to deliver Bangalore. Congress, held atDairy Tech. India 2013, at BIEC, South Asia’s 3rd Feed Dairy & Livestock Industry research paperatKottayam,Kerala. International Conferenceandtodelivertalkona (PDADMAS), Hebbal, Bangalore. Hebbal, (PDADMAS), of Animal and Disease SurveillanceMonitoring Software (SASforNARS)”atProjectDirectorate Training Programme on“DataAnalysis using SAS Dairy Products”heldatHotelHilton,Mumbai. National Seminaron “Mechanized Production of Indian cheese. milk, wheypowder,milk ediblecaseinand evaporated milk, sweetened condensed milk, fermented additives ofvariousstandards suchasmilkfat, food discussthe to Officials FSSAI with Meeting of Kashmir,Srinagar. Welfare” atSher-e-Kashmir, Univ. of Sci. & Technology for optimumLivestockProductionandHuman National Conferenceon“NutritionHealthInteractions Bangalore. Food SafetyManagement”atHindustanUnilever, Technical workshopon“RiskbasedApproachesfor of Kashmir,Srinagar. Security atSher-e-Kashmir,Univ.ofSci.&Technology Agricultural Growthfor Improving Rural Livelihood 21st AnnualAERAConference“Sustainable 23 6 2 30 16 7 24 9 24 2 1 19 18 10 th nd nd th st th October,2013 th th th rd th th th th -11 September,2013 -8 -3 -3 August,2013 September,2013 -28 - 21 September,2013 September,2013 -12 September,2013 th rd rd th September,2013 th September,2013 September,2013 st th August,2013 September,2013 September,2013 September,2013 Dr. A.K.Sharma,I/cComputer Dr. I.K.Sawhney,PS Mr. G.S.Meena,Sci. Dr. M.K.Singh,PS Dr. GautamKaul,PS Dr. ShaikAbdulHussain,Sci. Dr. K.JayarajRao,PS Dr. RajanSharma,PS Dr. B.C.Ghosh,PS Dr. P.N.Raju,Sci. Dr. P.N.Raju,Sci. Dr. S.Subash,PS Dr. M.C.A.Devi,PS Dr. PradipV.Behare,Sci. Dr. SurajitMandal,PS Dr. M.Manjunath,Sci. ERS Kalyani Dr. AnupamChatterjee,Sr.Sci. Dr. MohanMondal,Sr.Sci. Professor Dr.(Mrs.) MeenaMalik,Associate Dr. RajeshKumar,PS Dr. VivekSharma,PS MR. B.R.S.Murthy,Sci. Dr. S.Subash,PS Dr. S.Jeyakumar,PS Dr. M.Sivaram,PS Dr. B.Srinivas,PS Dr. K.P.Ramesha,PS Dr. P.K.Dixit,PS Dr. B.SurendraNath,PS Dr. C.N.Pagote,PS Dr. S.Kulkarni,Head Dr. K.Datta,Head Dr. M.S.Chauhan,PS Era HillsUniversityDehradun. in EngineeringApplication(ICMTEA-2013)”atGraphic International Conferenceon “Mathematical Techniques Processing atC.I.F.T,Cochin. and Pulsed Light Technology Technologies for Food National trainingon“ApplicationofHighPressure Deshmukh JNKVVCampus,Adhartal,Jabalpur,M.P. Gave a talk on Cell Sorting by flowcytometry at Nanaji talk atCMCLudhiana. Conference on“Medical Biochemistry” and todeliver Society ofIndia(DTSI)atHyderabad. by GLADEventsinAssociationwithDairyTechnology Made Presentation at“Dairy Summit2013”organized of IndustryandCommerce. BangaloreChamber Meridien, Bangaloreorganisedby Nutraceuticals &FunctionalFoods” atHotelLe Seminar on“TheFutureof Health&Wellness- & CIFT,Kochi. Incubation” conducted jointlybyNAARM, Hyderabad Hands onExperiencecumTraining“Business at Hyderabad. Development Units in NARS” organized by NAARM Orientation Workshopfor“BusinessPlanning& 8th NationalKVKConferenceatUAS,Bangalore. Health StatusandSocialWellBeing”atAAU,Anand 6th InternationalConference on“Fermented Foods, at IASRI,NewDelhi. Design for Development of Technology in Agriculture” Training programmeon“AdvancesinExperimental University, NewDelhi. (AFTENA- 2013)heldonatJawaharlalNehru Technologies &Environment–NewApproaches” 2nd InternationalConferenceon“AgricultureFood NAARM, Hyderabad. “Scientific ReportWritingandPresentation”at NAIP sponsoredNationalTrainingWorkshopon Sidharath, Delhi. Seminar on“FoodAnalysis”heldatHotelJaypee program OrganizedbyIDA(SZ)atBangalore. Dairying inIndia: “Driver for Agricultural Growth” Meghalaya. Eastern States” atAgricultural Universityof Barapani, National Seminar on “Agri-Business Potential for North College, Amritsar. person on“BasicTechniques……Cultures”atD.A.V. Delivered lecture intheworkshop to bearesource 24 18 21 14 9 7 25 24 November, 2013 23 6 23 19 17 14 11 8 29 th th th th th th th th th th th th th th rd rd st October,2013 -10 –9 November,2013 October,2013 -20 –31stOctober,2013 –22 October,2013 October,2013 November,2013 -30 October,2013 –26 –25 -25 Octoberto21 –– HumanResource Development –– th th November,2013 th th November,2013 th nd th th October,2013 October,2013 October2013 October,2013 October,2013 October,2013 st

133 ndri annual report 2013-14 134 ndri annual report 2013-14 –– HumanResource Development –– Dr. R.K.Malik,Head Dr. A.K.Mohanty,PS Dr. ArchanaVerma,PS Dr. DheerSingh,PS Dr. T.K.Datta,PS Dr. A.Manimaran,Sci. Dr. A.Kumaresan,Sr.Sci. Dr. R.C.Upadhyay,Head Dr. M.Sivaram,Sr.Sci. Dr. A.K.Sharma,I/cComputer Sh. PradeepBehare,Sci. Dr. KaushikKhamrui,Sr.Sci. Dr. RakeshKumar,Sr.Sci. Dr. S.K.Jha,Sr.Sci. Dr. S.Subash,PS Dr. M.C.A.Devi,PS Dr. A.K.Sharma,I/cComputer Dr. MukeshBhagat,Sr.Sci Dr. S.K.Tomer,Sr.Sci. Dr. SohanVirSingh,PS Dr. ParveenKumar,PS Dr. MahendraSingh,PS Dr. A.K.Sharma,I/cComputer Dr. I.K.Sawhney,PS Dr. B.C.Ghosh,PS Dr. RaghuH.V.,Sci. Dr.(Mrs.) LathaSabikhi,PS Dr. K.Ponnusamy,Head Dr. RaghuH.V.,Sci. Dr. R.K.Malik,Head Dr.(Mrs.) ShilpaVij,Sr.Sci. Dr. DheerSingh,PS organised byPunjabiUniversity,Patiala. International Conference on “Women and Development’’ New Delhi. National Seminar on ‘Crystallography’ at J.L.N.U. Allahabad. LalNehruNationalInstitute,Biotechnology atMoti ……Murrah Buffalo” organizedbyDepartment of Presented research paper on “Genetic Variability in Jammu. Drugs from Botanicals” atVeterinaryCollege,SKUAST, in TheraoeusticsandDevelopmentofAntiCancer Symposia on “Biopharmaceuticals and Nanotechnology 13th AnnualConferenceofISVPTandNational Asia” atKeralaForestResearchInstitute,Peechi. Volume andBiomassAllometricEquationsforSouth Discussion Meeting of the project “Inventory of Tree New Delhi. Sub Project“Developing…Examination”atASRB NAIP of aegis the under Officers Nodal of Training Health StatusandSocialWelfare” atAnand. 6th InternationalConferenceon “FermentedFood, University ofAgriculturalSciences, Bangalore. A GlobalPerspective” heldat Strategies for Sustainable Agricultural Development: 3rd InternationalConferenceon“ExtensionEducational Delhi. “Development of Web….Management” atIASRI, New Delivered expertlecture intheTraining programme DDUPC VV,Mathura. to AugmentFoodSecurityandAnimalWelfare”at on “Physiological and Nutri-genomic Interventions XII AnnualConferenceandNationalSymposium APIIT, Panipat. and CommunicationTechnologyICACCT-2013”at 7th InternationalConferenceon“AdvancedComputing College, Manipal. Interdisciplinary Collaboration”atKasturbaMedical Translational Research –PotentialBenefits of International HumboldtKollegon «BenchtoBedside Kundli. Food in Health and Disease Management” at NIFTEM, Conference on“Bioactive Compound & Functional Development” atNAARMHyderabad. Management Development Programme on“Leadership Relevance (FDMIR-2013)”atMDU,Rohtak. Innovations inMicrobiologyanditsInterdisciplinary International Symposium on “Frontier Discoveries and Kurukshetra. by JawaharNavodayaVidyalaya atNiwarsi, Distt. Regional LevelScienceCongress–2013organised Yeast-2013 atChandigarh. Conference on“8thInternational University, Varanasi. Reproductive Health,Zoology”atBanarasHindu A NationalSymposiumon“RecentAdvancesin 23 21 21 20 18 21 6 5 30 19 16 15 15 26 17 16 7 4 28 th th th th th th th th th th th th th th rd st st st th -7 -8 -7 December2013 -23 -23 - 22 –21 -19 –21 November,2013 November,2013 -17 -16 November,2013 -30 November,2013to –22 November,2013 th th th December,2013 December,2013 December,2013 th rd rd nd th th th November,2013 st st nd November,2013 November,2013 November,2013 November,2013 November,2013 November,2013 November,2013 November,2013 November,2013 Dr. BimleshMann,Head Mr. K.L.Sampath,Sci. Mrs. VedavathiM.K,Sci. Mrs.Vimala, Sci. Dr.(Mrs.) ShilpaVij,Sr.Sci. Dr. S.Subash,PS Dr. S.Varalakshmi,PS Dr. M.Sivaram,PS Dr. B.Srinivas,PS Dr. K.P.Ramesha,PS Dr. P.K.Dixit,PS Dr. C.N.Pagote,PS Dr. S.K.Kanawjia,PS Dr. K.S.Kadian,PS Dr. SohanVirSingh,PS Dr. S.Kulkarni,Head Dr. R.K.Malik,Head Dr. A.Kumaresan,Sr.Sci. Dr. R.C.Upadhyay,Head Dr. M.A.Kataktalware,PS Dr. S.K.Tomar,PS Dr. S.Varalakshmi,PS Dr. P.HeartwinAmaladhas,PS Dr. A.K.Dang,Sr.Sci. Dr. RajeevKapila,Sr.Sci. Sh. VishalAcharya,AF&AO Dr. I.D.Gupta,PS Dr.(Mrs.) JancyGupta,PS Dr. V.K.Gupta,Head Dr. ParveenKumar,PS Sh. S.George,Comptroller Dr.(Mrs.) MadhuMohini,PS …..Foods” atIIT,Madras. Presented researchproposalentitled“Preparationand (South zone)atChennaiTradeCentre,Chennai. 42nd DairyIndustry ConferenceorganizedbyIDA Programme” atMadrasVeterinaryCollege,Chennai. Sensitization on“E-courseforB.V.Sc.&AHDegree Pradesh. Dirang, Arunachal System” at Transhumance Rearing Yaks” duringtrainingprogrammeon“YakWelfarein Guest Speakeronthetopic“EnvironmentalStressons Bangalore. Society ofExtensionEducationatUAS,GKVK, Sciences, BangaloreandInternational of Agricultural the University Perspective” organizedby – AGlobal Strategies forSustainable Agricultural Development International Conferenceon“ExtensionEducational Research (DRR),Rajendranagar,Hyderabad. & NutritionalSecurity”heldat DirectorateofRice Consultation on“MilletsPromotionforHealth Global Institute andpresentedaposter. Organized byCentral Food Technological Research IFCON International Conference held at Mysore University, Triputi(AP). Biotechnology &HealthTherapecutics”atS.V. Societies onPhysiologyEthnopharmacology 5th Congress of Federation of India Physiological NIFM, Faridabad. Special TrainingProgrammeon“FinancialIssues”at CIFE, Mumbai. and AlliedSciences:Achievement&Challenges”at National Seminaron“NewTechnologyofAgricultural projects underNAIPatNewDelhi. Workshop on“Cross Cutting Experiences”inICTsub- Trade Centre,Chenai,TamilNadu. “Indian Dairying:GrowthandTradeIssues”atChennai General Body Meeting andtheAnnualConferenceon Information SystemandAudit(ICISA)Noida. & AuditSentization”atInternationalCentrefor Training Programmeon“FinancialManagement Improved AnimalHealthandNutrition”atLucknow National Symposiumon“ProductivityEnhancement 13 12 10 9 5 18 18 17 16 14 6 12 9 13 th th th th th th th th th th th th th th –13 December,2013 -8 -7 -14 -14 December,2013 -20 -19 -19 –20 –16 –15 December,2013 –– HumanResource Development –– th th December,2013 December,2013 th th th th th th th th th December,2013 December,2013 December,2013 December,2013 December,2013 December,2013 December,2013 December,2013 December,2013 135 ndri annual report 2013-14 136 ndri annual report 2013-14 –– HumanResource Development –– Dr. K.JayarajRao,PS Dr. BimleshMann,Head Dr. A.K.Singh,Sr.Sci. Dr. NareshKumar,Sr.Sci. Dr. Y.S.Rajput,Head,ABC Dr. K.P.Ramesha,PS Dr. Raghu,H.V.,Sci. Dr. NareshGoel,PS Dr. RajanSharma,PS Dr. Y.S.Rajput,Head Dr. Rubina Kumari Baithalu, Sci. Dr. T.K.Mohanty,PS Dr. K.S.Kadian,PS Dr. RajanSharma,PS Dr. YogeshKhetra,Sci. Sh. SathishKumarMH,Sci. Dr. RaghuH.V.,Sci. Sh. DevarajaH.C.,Sci. Dr. VijayKumarGupta,PS Dr. BimleshMann,Head Dr. A.Kumaresan,Sci. Dr. ShivPrasad,PS Dr. K.Datta,Head Professor Dr.(Mrs.) MeenaMalik,Associate PS Dr. F.MagdalineEljeevaEmerald, Dr. P.HeartwinAmaladhas,PS Dr. M.Manjunatha,PS Dr. Rashmi.H.M.,Sci. Dr. M.Mondal,Sr.Sci. Dr. A.K.Mohanty,PS Dr. M.S.Chauhan,PS Dr. ChandRam,Sci. Dr. NareshKumar,Sr.Sci. Dr. DheerSingh,PS Delhi. on NutritionandHealth(IFCIN-2013)atJNU,New International Conferenceon“FoodTechnologyImpact at NASCComplex,NewDelhi. Workshop on“ValuationandPricingofTechnologies” Animal GeneticResources”atNASC,NewDelhi. “Conservation andProductivityEnhancementoffarm to related onStrategy Brainstorming Workshop Workshop on Food Safety & Quality”, NIFTEM, Kundli. Safety Risks:ChallengesforDevelopingCountriesand 2nd InternationalConferenceon“Emerging Food Reproduction” atNagpur. Convention oftheIndian Society for Study of Animal Fecundity: GlobalPerspectiveanXXIVAnnual and forEnhancingAnimalFertility Biotechnology National Symposium on “Fronties Reproductive Security” atHAU,Hissar. Agricultural ResearchtoensureNationalFood National Seminaronthetheme“Reorientationof on CamelatBikaner. Buffalo, Goat and Sheep” at National Research Center Milk as Compared to Milk of different Species – Cow, Brainstorming Meet on“FunctionalValueofCamel Karnataka. of FoodScientistsandTechnologists (India), Mysore, Research & Education for Health Foods” Association “Nutritional SecuritythroughSustainableDevelopment 7th InternationalFood Convention (IFCON-2013) on and HealthofTharparkarCattle”atCAZRI, Jodhpur. Interface meetingon“ImprovementofProduction of ColcattaatISI,Kolkata. Diversity and Democracy” at Department of Eco. Uni. Annual InternationalConferenceon“Education organized by Department of Humanities at NIT,Trichy. at ofHumanities Department by organized Language Teaching:Challenges andOpportunities” Workshop on“GlobalTrendsand IssuesinEnglish (ICAR), Bangalore. 3rd InnovativeandProgressiveFarmersMeetatNIANP Delhi. Advances in Bioinformatics for‘omics’Data”atNew CAFT programmeon“ComputationalandStatistical Institute ofAnimalBiotechnology,,Hyderabad. atNationalEnhancement ofLivestockProductivity” Workshop on“ReproductiveBiotechnologiesfor India HabitatCentre,LodhiRoad,NewDelhi. Conference on “Food Safety and Environment” Toxin 04 atGoa. No.C4/4180001- Computer onBiosensorNAIPProject Birla Goa BITS, Pilani-KK proposed at CAC Meeting Training” underNAIPatPUSA,NewDelhi Workshop on“PanelwithCourseDirectorsofNational 23 26 10 9 8 6 3 18 10 5 28 24 22 21 20 20 18 13 th th th th th th th rd th th th th st th th th th th -7 -7 January,2014 –11 –10 Januaryto –30 December,2013 -21 –26 January,2014 February,2014 -21stJanuary,2014 -21stJanuary,2014 January,2014 January,2014 January,2014 – 24 th th January,2014 January,2014 th th st th th th December,2013 January,2014 January,2014 December,2013 December,2013 January,2014 Dr. SumitArora,PS Dr. M.Bhakat,Sci. Dr. M.A.Kataktalware,PS Dr. PawanSingh,PS Dr. RavinderMalhotra,PS Dr. A.K.Sharma,I/cComputer Dr. ArchanaVerma,PS Dr. Avtarsingh,PS Dr. D.Malakar,PS Dr. ArchanaVerma,PS Dr. DheerSingh,PS Dr. MukeshBhagat,Sci. Dr. A.Manimaran,Sci. Dr. Rubina Kumari Baithalu, Sci. Dr. M.S.Chauhan,PS Dr. DheerSingh,PS Dr. SohanVirSingh,PS Dr. K.P.Ramesha,PS Dr. Raghu,H.V.,Sci. Dr. RajanSharma,PS Dr. DheerSingh,PS Dr. PradyumanBarnwal,Sr.Sci. Dr. A.Kumaresan,Sci. Dr. T.K.Mohanty,PS Dr. ShilpaVij,PS Dr. K.P.Ramesha,PS Dr. RakeshKumar,Sr.Sci. Dr. MaganSingh,Sr.Sci. Mr. Chitranayak,Sci. Dr. A.Kumaresan,Sr.Sci. Dr. A.K.Roy,Sr.Sci. Dr. T.K.Mohanty,PS Dr. K.Ponnusamy,Head Dr. Rashmi.H.M.,Sci. Dr. R.K.Malik,Head Panchkula. in Haryana“EnrichingFoods,EnrichedFoods”at fortification food staple on consultation Stakeholder Agriculture University,Anand. Science&AHAnand,Anand at CollegeofVeterinary of IndianSocietyAnimal Production&Management under EraofClimateChange”&XXIAnnualConvention for Livestock Productivity & Profitability Enhancement National Seminaron“NewDimensionalApproaches Vishwa Vidayala,Sonepat. Computer & Application” at Bhagat Phool Singh Mahila ofStatistics AnnualConference of“Society 16th Pune. Bioinformatics” at YashadaAuditorium, Banner Road, &International Conferenceon“Biotechnology for UpliftmentofRuralMasses”atNBAGR,Karnal. and GenomicsforSustainableManagementofLivestock XI NationalSymposiumon“HarmonizingPhenomics of Jammu,Jammu. 101st Indian National Science Congress at University Bareilly. & 24thAnnualMeetingofISSRF”atIVRI,Izatnagar, Issues andStrategiesunderChangingClimateScenario International Conference on “Reproductive Health: and Biotechnology”atMysore. International Conference on“Biodiversity, Bioresources Products” organizedbyPCDF,Lucknow. Quality AssuranceandMarketing ofMilkand State LevelSeminaron”DairyAnimal Management, Hindu University,Varanasi. of Zoology,Banaras on “AnimalsofLASAI”atDept. Delivered a plenary lecture at the National Symposium Lal NehruUniversity,NewDelhi. Automobile and Aeronautical Engineering” atJawahar Mechanical Material Manufacturing Automotive, International Conference on “Innovative Trends in AIIMS, NewDelhi. Advisory CommitteeMeetingofNFBSFARAProjectat Nutraceuticals” held atSLIET,Longowal. in theDevelopmentofFunctional Foods and National Conference on“InnovativeTechniques Industry. ofIndian Confederation by organized Bangalore at “Interfacing InnovationandIPRforBusiness Success” IPR Summit-ConferenceonInnovationand Accelerating theImpactsofAgro-forestry”atDelhi. World Congresson“Agro-forestry“TreesforLife Researchers” atNAARMHyderabad. Workshop on“TechnologyManagementfor Five day NAIP Sponsored Management Development in Governance”atISTM,NewDelhi. Training on“ScienceTechnology&EmergingTrends at NewDelhi. Gut Function–Transforming Health andWell-Being” Probiotic symposiumon“Probiotics,Microbiomeand 6 28 24 1 6 3 6 30 20 16 15 15 14 13 10 27 3 15 th rd rd th st th -2 th th th th th th th th th th th th – 7 February,2014 –8 –7 –7 -31 -30 –31 -26 –21 -18 -16 -16 February,2014 –15 -14 –14 –– HumanResource Development –– nd th th th th February,2014 February,2014 February,2014 th th th th th th February,2014 February,2014 st st th th st January,2014 February,2014 February,2014 February,2014 February,2014 February,2014 January,2014 February,2014 January,2014 February,2014 February,2014 137 ndri annual report 2013-14 138 ndri annual report 2013-14 –– HumanResource Development –– Dr. M.Mondal,Sr.Sci. Dr. J.K.Kaushik,Sr.Sci. Dr. A.K.Mohanty,PS Dr. M.Mondal,Sr.Sci. Dr. K.Karunakaran,Sr.Sci. Dr. M.Mondal,Sr.Sci. Dr. A.Chatterjee,Sr.Sci. Dr. A.Mandal,Sr.Sci. Dr. C.Bhakat,PS Dr. A.Santra,PS Dr. SubrataK.Das,PS Dr. M.K.Ghosh,PS Dr. T.K.Dutta,PS Dr. B.SurendraNath,PS Dr. R.K.Malik,Head Dr. V.K.Gupta,Head Dr. M.S.Chauhan,PS Dr. DheerSingh,PS Dr. S.K.AtrejaPS Dr. M.S.Chauhan,PS Dr. A.K.Mohanty,PS Dr. P.N.Raju,Sci. Dr. Y.S.Rajput,Head Dr. J.K.Kaushik,Sr.Sci. Dr. M.S.Chauhan,PS Dr. V.K.Gupta,Head Dr. A.K.Chakravarty,PS Dr. DheerSingh,PS Dr. BimleshMann,Head Dr. NareshKumar,Sr.Sci. Dr. SumitArora,PS Dr. LathaSabikhi,PS Dr. R.K.Malik,Head Mithun, Nagaland Interface MeetingandMithunFestival heldatNRCon NAARM, Hyderabad. Project DevelopmentWorkshopofNationalFundat and FisheryScience(WBUAFS). Faculty ofDairy Technology, WB University of Animal in India –Post OperationFlood Scenario”organized by National Seminar on “Innovations in Dairy Processing NAARM, Hyderabad Workshop on“TechnologyEnhancedLearning”at and Cloning”atM.D.U.Rohtak. National Seminaron“Advances in StemCellResearch Gujrat. Department ofAnimalBiotechnology,AAU,Anand, National Training program on“Bioinformatics” at Anusandhan Sansthan,Mathura(UP). Evam Go- Vishwavidyalaya Vigyan Pashu Chikitsa and AnimalHusbandry,PanditDeenDayalUpadhyaya Veterinary Biochemistry CollegeofVeterinaryScience in AnimalHealthandProductionatDepartmentof National Seminaron“BiotechnologicalChallenges College atChennai. Systematic Approach to Training at Madras Veterinary Health &Production”atAH,DUVASU,Mathura. technological ApproacherstoChallengersinAnimal & Bio-technologyNationalSeminaron“Bio- 2nd AnnualMeetingofSocietyVeterinaryScience Institute ofOceanography”,DonaPaula,Goa. 25th BTisnetAnnualcoordinatormeetingat“National Medicine” atMDU,Rohtak Delivered guestlecture,“StemCellandHuman New Delhi. NAIP atNASCComplex, Component-1” organizedby for ManagementofChangeintheIndianNARS: Workshop on“ICARastheCatalyzingAgent in IndiaPostOperationFloodScenario”atNadia(WB) National Seminaron“InnovationinDairyProcessing Nanotechnology” atCAZRIJodhpur. Training Programme on“Application of Safety ofDairyProcessingIndustry”atNewDelhi. National Seminar on “Enhancing Productivity & Food Varanasi. organised byNational Academy of Dairy Science BHU, and Processing Technologies inEastern India” Meeting onProspectsandProblemsinDairyProduction Expert ConsultativeMeeton“ExpertConsultation 27 20 14 14 8 7 6 25 6 26 1 7 3 1 14 13 13 10 th th th th st rd st th March,2014 March,2014 th th th th th th th th th -8 March2014 -7 -7 March,2014 March,2014 th Februaryto Februaryto -28 -22 -15 -15 March,2014 March,2014 March,2014 -15 th th th March,2014 March,2014 March,2014 th nd th th th March,2014 March,2014 March,2014 March,2014 March,2014

ORKSHOPS/ CONFERENCES/SEMINARS/SYMPOSIA/WORKSHOPS/ 12 participation ofdelegatesfromIndiaandabroad.Sometheimportantonesarelistedasunder: The Institute hosted quite a good number of Seminars, Workshops and Short Courses with the Workshop onFMS/MIS. Entrepreneurship DevelopmentProgrammeon“CommercialDairyFarming”. and MilkProducts”. CAFT TrainingProgrammeon“InnovativeApproachesinProcessingandPackagingofMilk Brain StormingSessionon“BuffaloEstrusBiology”. Awareness Programme for Developing the Product “Multiple Micro-Nutrient Fortified Milk”. Training Programmeon“PasupalanVikasHetuCharaUtpadanEvamSanrakshan”. Brain Storming Session on “Transition Cow Management to Optimize Reproduction Efficiency”. Rajbhasha MukhyaSamaroh. Brain StormingSessionon“MilkGenomicsandHumanHealth”. of TraditionalIndianDairyProducts”. Training Programmeon“AdvancesinProduction,Functional,RheologicalandQualityAspects Rajbhasha Karyashala. Consultative MeetingofPricingMilkorganizedbyHaryanaKisanAyogatNDRI,Karnal. XVII NationalConferenceofAgriculturalStatistics. Dairy Foods”. Five DayTrainingProgrammeon“HandsTechnological AspectsofComposite World VeterinaryDay. National TrainingProgrammeon“ClimateResilientLivestockandProductionSystem”. Foundation DayofModelDairyPlant. Orientation ProgrammeforNewlyAdmittedStudents. Training Programme on “Chemical and Microbiological Quality Assurance for Dairy Supply”. and AlliedSciences”. One Day Awareness Programme and Workshop on “Intellectual Property Rights in Agriculture Narakas KarnalKeSadasyaKaryalayaHetu5DivseeyaAnuvaadPrashikshanKaryakaram. Expert ConsultativeMeetonProteinMalnutrition. Short Courseon“RecentAdvancesProteomicsforBiomarkerDiscovery”. Foundation DayCelebrationsofNDRIatSRS. National Symposiumon“ChildNutritionSecurityinIndiaWayForwardwithMilk”. World MilkDay. Training Programmeon“CommercialDairyFarming”. tRAINING pROGRAMMES/FIELDDAYSORGANISED Prof. V.N.Sharda,memberASRBaddressing thefacultyofNDRIatWorldMilkDayon1

7 26 7 28 27 18 16 11 7 9 28 31 27 December, 2013 23 27 18 2013 17 2 1 1 15 2013 6 2013 8 1 1 1 4 th th nd th st st st st th th th st th st July,2013 June,2013 June,2013 -7 October,2013 th th th st th th th rd th th th th th th –8 August,2013 -17 November,2013 November,2013 -13 –28 August,2013 June,2013 May,2013 September,2013 –19 –29 April,2013 April,2013 –28 Novemberto1 –22 November,2013 November,2013 September,2013 September,2013 -27 th th th May,2013 th th August,2013 July,2013 November, th th th th nd October,2013 April,2013 July,2013 April,2013 November, November, st

139 ndri annual report 2013-14 140 ndri annual report 2013-14 –– Conferences/ Seminars/ Symposia/Workshops/ Training Programmes/ FieldDaysOrganised –– Livestock Productivity”. CAFT TrainingProgrammeonAdvancedBreedingandAlliedTechnologies forEnhancing National DairyMela. Dr. D.SundaresanMemorialOration. N. DasturMemorialOration. Seminar on“EvolutiontoRevolutioninDairyEducation”. Training Programmeon“CommercialDairyFarming”. Dr. K.IyaMemorialOration. at SRS,Bangalore. Model TrainingCourseon“NewExtensionStrategiesforSustainableDairyDevelopment” Brain StormingSessionon“MicronutrientInitiative:ASilentSolutiontoHiddenHunger”. Directorate ofDairyDevelopment,Kerala. Short Trainingon“AnalysisofMilk,MilkproductsandCattleFeed”forDairyPersonnelfrom Development andProblemsofDairyFarmersinHaryana”. on “Cattle Stakeholders Meeting Degree programme. National WorkshopunderNAIPComponent-I“Developmentofe-courses”forB.Tech.(DT) Training Programmeon“StemCells”. Entrepreneurship DevelopmentProgrammeon“CommercialDairyFarming”. Brain StormingSessionon“PotentialofAnimalSystemforCLAEnhancement”. Production”. Training Programme on“Advanced Training onCloningResearch forQualityBuffalo Business MeetofDDMSNABARDHaryanaRegionalOffice. ERS, NDRI,Kalyani. Training programmeon“CleanMilkProduction”forTribalWomenFarmersunderTSPat National ScienceDay. NDRI-Industry Meet. Convocation ofNADSIandSeminaron“ClimateResilientDairying”. Dr. R. S. Paroda addressing the delegates during XVII National Conference of Agricultural Research Statisticians on Dr. R.S.Parodaaddressingthedelegates duringXVIINationalConferenceofAgriculturalResearchStatisticianson "National PrioritiesinAgriculturalStatistics andInformatics" 5 25 12 10 31 29 28 27 24 10 8 8 2 2013 10 18 13 2013 24 11 28 6 2014 8 7 th th th th th nd th st th th th th th th th th th th th th th th February,2014 February,2014 January,2014 January,2014 January,2014 –25 –23 January,2014 Januaryto January,2014 January,2014 January,2014 January,2014 –22 –17 –13 March,2014 –14 February,2014 –27 February,2014 February,2014 th rd nd th th th th March,2014 December, January,2014 December, March,2014 February, January,2014

13 29.09.2013 22.08.2013 18-19.8.2013 09.07.2013 21-22.05.2013 DISTINGUISHED VISITORS Programme (WAAPP). ProductivityAfrica Agricultural Nigerian delegationunderWest Husbandry Commissioner. India and Dairying &Fisheries, Govt.of Department ofAnimal Husbandry, Sh. A. K. Thakur, Delhi. for SecretaryGeneral,AARDO, New Division Research Head, & Officer led byDr.Khushnood Ali,Technical of Bangladesh,Camilla, Bangladesh Government ofthePeople’sRepublic Rural Development & Cooperative Minister ofLocal Government, for RuralDevelopment(BARD), Academy Bangladesh (Training), General, Mr. Md.MashiurRahman,Director United States of America, NewDelhi. Agricultural Specialist, Embassy of alongwith Counsellor forAgriculturalAffairs Mr. Allan Mustard,Minister- Centre, TheNetherlands. Wageningen University and Research Centre forGeneticResources ResourcesGroupAnimal Genetic Mr. SipkeJoostHiemstra,Head, , Director Dr. KamrulAhsan,Director Dr. A. S. Nanda, Animal Dr. Santosh Singh,Sr. Secretary, 29.03.2014 18.03.2014 13.02.2014 10.02.2014 03.02.2014 30.01.2014 16.01.2014 7-8.01.2014 24.10.2013 5. 4. 3. 2. 1. 01.10.2013

Veterinary andAnimalSciences. Lala Lajpat Rai University of Newly appointedfacultymembers, Pvt. Ltd. India Danone Yakult from Officials Iwama San and Mr. Vipin Biala- NARC, Nepal. Twelve member delegation from Gerais, Brazil. Universidade FederaldeMinas Mendonca and Dr. Mario Gracia, Dr. RodrigoMartinsAlvesde Government ofBhutan(RGOB). for Agriculture and Forest, Royal H. E.LyonpoYesheyDorji, Minister Australia 2014. Dr. GregoryHarperfromCSIRO, from USA. Twelve member student delegation with NDRI,Karnal. UPSC, WinterStudyTourattached IAS Officer Trainees (2013 batch), IAS, Animals (WSPA). World SocietyforProtectionof from delegation Fifteen member Secretary, FijiHighCommission. Mr. OmPrakash Goundar, Second Dr. GaneshChand,ViceChanceller. National DisasterManagement. Secretary forRuralMartimeDev.& Mr. FilipeAliferite,Permanent Commissioner. Mr. YogeshJKaran,High Fishries &Forest. Seruirato, Minister for Agriculture, His Excellency Mr.IniaBatikoto FIJI Delegation 141 ndri annual report 2013-14 142 ndri annual report 2013-14 14 PERSONNEL Om VirSingh,M.V.Sc.,Ph.D. Avtar Singh,Ph.D. I. D.Gupta,Ph.D. Archana Verma,Ph.D. A. K.Chakravarty,Ph.D. A. K.Gupta,Ph.D. Dairy CattleBreedingDivision Scientists andTechnicalOfficers R. A.Dey,Ph.D. P. K.Dixit,Ph.D. Meena Malik,M.Phil.Ph.D Y. S.Rajput,Ph.D. J. K.Kewalramani,B.Sc.,M.B.A. RTI Cell Ranjana, BA Shukantla Rani,B.A Simita Roy,B.A Nirmala Kumari,B.A Parvesh Lata,B.A Prem Kumari,B.A Shukla Vermani,B.A Sunita Chaudhary Deepak Chopra,B.A. Nirmal KumarJain,B.A. Dharam SinghMeena,B.A. Mukesh KumarDua,B.A. Braham Prakash,B.A. Rajbir, B.A. N. K.Verma Saroj Khurana,M.A. S. Meena,B.A. S. ShashiKala Ishwar Diyal,B.A. Ram Niwas,B.A. R. K.Bansal,M.A. A. G.Barapatre Kuna RamKisku Vishal Acharya,MA Ram Shankar,M.A.,P.G.D.T. Sarita Yadav,B.P.T.,PGDPMIR Mithlesh Kumar,M.Sc.,PGDCFA Ashok Mallick,B.Com. R. C.Meena,M.Com. I. K.Sawhney,M.Tech. S. K.Kanawjia,Ph.D. S. George,M.A.,PGDLA & Registrar J. K.Kewalramani,B.Sc.,M.B.A. G. R.Patil,Ph.D. G. R.Patil,Ph.D. A. K.Srivastava,Ph.D. General Administration Asstt. Admn.Officer(SRS,Bangalore) Private Secretary(ERS,Kalyani) Acting JointDirector(Research) Asstt. Pub.InformationOfficer Asstt. Pub.InformationOfficer Admn. Officer(ERS,Kalyani) Asstt. Fin.&AccountOfficer Public InformationOfficer Joint Director(Academic) Chief TechnicalOfficer Academic Coordinator Joint Director(Admn.) Asstt. Admn.Officer Asstt. Admn.Officer Asstt. Admn.Officer Asstt. Admn.Officer Asstt. Admn.Officer Asstt. Admn.Officer Asstt. Admn.Officer Asstt. Admn.Officer Asstt. Admn.Officer Asstt. Admn.Officer Asstt. Admn.Officer Asstt. Admn.Officer Appellate Authority Controller ofExams Information Officer Principal Scientist Principal Scientist Principal Scientist Principal Scientist Sr. Admn.Officer Sr. Admn.Officer Private Secretary Private Secretary Private Secretary Private Secretary Private Secretary Private Secretary Private Secretary Deputy Director Account Officer Security Officer Admn. Officer Acting Head Comptroller (Off. Lang.) Asstt. Pub. I Sr. Fin.& nstitute Director Y. P.Singh,B.Sc. Vikram Mool Chand Ashwani Kumar,M.Sc. Rakesh KumarTonk,Ph.D. Y. K.Panwar,M.A. Vinod Kumar,B.Sc. Karan Singh,M.Sc. Raj Bahadur,B.Sc. Goutam Mondal Nitin Tyagi, Chander Datt,Ph.D. P. S.Oberioi,Ph.D. A. K.Tyagi,Ph.D. Veena Mani,Ph.D. S. K.Tomar,Ph.D. Madhu Mohini,Ph.D. S. Thakur,Ph.D. Neelam Kewalramani,Ph.D. J. P.Sehgal,Ph.D. S. Kundu,Ph.D. Dairy CattleNutritionDivision Shiv Kumar,M.Sc. Prasant Kumar,M.Sc. A. Manimaran,M.Sc. Ramesh Chandra,Ph.D. A. Kumaresan,Ph.D. S. Lathwal,Ph.D. M. L.Kamboj,Ph.D. T. K.Mohanty,Ph.D. R. K.Mehla,Ph.D. Shiv Prasad,Ph.D. Livestock ProductionandManagementSection A. K.Mohanty,Ph.D. J. K.Kaushik,Ph.D. S. De,Ph.D. T. K.Datta,Ph.D. M. S.Chauhan,Ph.D. S. K.Singla,Ph.D. P. Palta,Ph.D. R. S.Manik,Ph.D. Animal BiotechnologyCentre Ashwani KumarRoy,Ph.D. Ashutosh, Ph.D. Manju Ashutosh,Ph.D. Anjuli Aggarwal,Ph.D. A. K.Dang,Ph.D. N. AnandLaxmi,Ph.D. Sohanvir Singh,Ph.D. Mahendra Singh,Ph.D. Parveen Kumar,Ph.D. Sujata Pandita,Ph.D. O. K.Hooda,Ph.D. R. C.Upadhayay,Ph.D. Dairy CattlePhysiologyDivision S taff Principal ScientistandIn-charge Principal Scientist&Incharge Asstt. ChiefTechnicalOfficer Asstt. ChiefTechnicalOfficer As on31 Sr. TechnicalOfficer Sr. TechnicalOfficer Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Technical Officer Technical Officer Technical Officer Technical Officer Technical Officer Technical Officer st March, 2014 Sr. Scientist Sr. Scientist Sr. Scientist Sr. Scientist Sr. Scientist Sr. Scientist Sr. Scientist Sr. Scientist Sr. Scientist Sr. Scientist Scientist Scientist Head Head Y. S.Rajput,Ph.D. Animal BiochemistryDivision D. Malakar,Ph.D. Shilpa Vij,Ph.D. Naresh Kumar,Ph.D. S. K.Tomar,Ph.D. A. K.Puniya,Ph.D. Sunita Grover,Ph.D. R. K.Malik,Ph.D. Dairy MicrobiologyDivision P. C.Singh,M.Sc. Rajesh Kumar,Ph.D. Rajan Sharma,Ph.D. Vivek Sharma,Ph.D. Sumit Arora,Ph.D. Raman Seth,Ph.D. Darshan Lal,Ph.D. Bimlesh Mann,Ph.D. Dairy ChemistryDivision Prathvi Raj Subhash Chander,Dip.(Electrical) Sanjeev Kumar,M.A. Probir Mondal,B.Sc. Officer A. K.Kohli,Dip.(Mech.Engg.) Hari RamGupta,M.Sc. A. K.Sharma,B.Sc. Experimental Dairy G. K.Goyal,Ph.D. Lehri Singh,M.Sc. Devaraja, H.C.,M.Sc. Sathish Kumar,M.H.,M.Sc. Ganga SahayMeena,M.Sc. Yogesh Khetra,M.Sc. Prateek Sharma,M.Sc. Narender RajuPanjagari,Ph.D. Kaushik Khamrui,Ph.D. A. K.Singh,Ph.D. Latha Sabikhi,Ph.D. S. K.Kanawjia,Ph.D. Vijay Kumar,Ph.D. Dairy TechnologyDivision Shisha Singh Ravi Kant,M.Sc. Ms. SuneetaMeena,M.Sc. Sunil KumarOnteru,Ph.D. S. K.Sood,Ph.D. Suman KapilaPh.D. Rajeev Kapila,Ph.D. Dheer Singh,Ph.D. Gautam Kaul,Ph.D. R. K.Sharma,Ph.D. S. K.Atreja,Ph.D. S. Kumar,Ph.D. M. K.Singh,Ph.D. Rajesh Kumar Asstt. ChiefTechnicalOfficer Asstt. ChiefTechnicalOfficer (Chief TechnicalOfficer) Chief TechnicalOfficer Asstt. ChiefTechnical Dairy Superintendent Sr. TechnicalOfficer Sr. TechnicalOfficer Sr. TechnicalOfficer Emeritus Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Technical Officer Technical Officer Technical Officer Sr. Scientist Sr. Scientist Sr. Scientist Sr. Scientist Sr. Scientist Scientist Scientist Scientist Scientist Scientist Scientist Scientist Scientist Scientist Head Head Head Head Tara Chand,B.Sc. Rishi KantaSingh,M.Sc. Uditya Chaudhary,Ph.D. A. K.Sharma,Ph.D. Ravinder Malhotra,Ph.D. Smita Sirohi,Ph.D. A. K.Chauhan,Ph.D. B. S.Chandel,Ph.D. K. Datta,Ph.D. Division Dairy Economics,StatisticsandManagement Pawan Kumar J. K.Dabas,M.Tech. Sunil Kumar,M.Sc. S. K.Chaudhary,A.M.I.E. Om Prakash,Diploma(Agri.Engg.) Prashant SaurabhMinz,M.Tech. P. Barnwal Bikram Kumar,M.Tech. I. K.Sawhney,M.Tech. A. K.Dodeja,Ph.D. Dairy EngineeringDivision Saroj Mehta,M.Sc.,B.Ed. Surender Gupta,Ph.D. Dalip K.Gosain,Ph.D. Centre Krishi VigyanKendra/Trainers’ Training Rajpal Sharma,B.A. Pramod Kumar,M.Sc. Kumar Bharat,M.A. P. S.Oberoi,Ph.D. (ATIC) Agricultural TechnologyInformationCentre Jai BhawanSharma,Dip.In(AH) Mridula Upadhayay,M.A. Parvinder Sharma,Ph.D. Asif Mohammad,Ph.D. R. SenthilKumar,Ph.D. Ritu Chakravarty,M.Sc. H. R.Meena,Ph.D. B. S.Meena,Ph.D. Gopal Sankhala,Ph.D. S. K.Jha,Ph.D. K. S.Kadian,Ph.D. Khajan Singh,Ph.D. Jancy Gupta,Ph.D. K. Ponnusamy,Ph.D. Dairy ExtensionDivision V. K.Batish,Ph.D. Chamela Ram R. P.Singh,Ph.D. Rashmi H.M.,M.Sc. P. V.Behare,Ph.D. Raghu H.V.,M.Sc. Surjit Mandal,Ph.D. Chand Ram,Ph.D. Principal Scientist&Incharge Chief TechnicalOfficer Chief TechnicalOfficer Chief TechnicalOfficer Chief TechnicalOfficer Chief TechnicalOfficer Chief TechnicalOfficer Progam Coordinator Sr. TechnicalOfficer Sr. TechnicalOfficer Sr. TechnicalOfficer Sr. TechnicalOfficer Sr. TechnicalOfficer Emeritus Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Technical Officer Technical Officer Technical Officer Technical Officer Chief Technical Scientist (SG) Scientist (SS) Sr. Scientist Sr. Scientist Sr. Scientist Sr. Scientist –– Personnel –– Scientist Scientist Scientist Scientist Scientist Scientist Scientist Scientist Officer Head Head Head 143 ndri annual report 2013-14 144 ndri annual report 2013-14 Dheer Singh,Ph.D. S. V.Singh,Ph.D. Gopal Sankala,Ph.D. A. K.Tyagi,Ph.D. I. K.Sawhney,Ph.D. S. K.Kanawjia,Ph.D. G. R.Patil,Ph.D. University Office Amarpal Singh,M.Sc. Subhash Chand,B.V.Sc. Jintendra Rana,M.Sc. R. T.Bansode,M.Sc. J. K.Pundir,B.V.Sc. Mukesh Bhakat,Ph.D. T. K.Mohanty,Ph.D. Pawan Singh A. K.Gupta,Ph.D. A. K.Chakravarty,Ph.D. Artificial BreedingResearchCentre Sahdev Singh,M.Sc. Praveen Kumar,M.V.Sc. K.P.S. Tomar,M.V.Sc. M. K.Srivastava,M.V.Sc. Animal HealthComplex Deepa Kumari,B.Sc.(HSc),MA Chaman Lal,Dip.(MotorMechanic) S. Raju,M.V.Sc. Rubina KumariBithalu Nishant Kumar,M.Sc. A. Manimaran,M.Sc. A. Kumaresan,Ph.D. Ramesh Chandra,Ph.D. M. L.Kamboj,Ph.D. T. K.Mohanty,Ph.D. S. Lathwal R. K.Mehla Shiv Prasad,Ph.D. Livestock ResearchCentre Mr RameshChand Dr RakeshKumar,B.Sc.(Agri.) Dr MaganSingh,Ph.D. Forage ResearchandManagementCentre Ravi Rawat,M.Sc.(Entomology) Sukhdev Singh Anil KumarDagar,M.Sc. Ramesh Chand,B.Sc.(Agri.) Uttam Kumar,Ph.D. Satish Kumar,M.Sc.(Horti.) Ashutosh, Ph.D. Forage Section Prem Singh D. V.Sharma Kulvir Singh,M.Sc. Mohar Singh,M.Sc. Rajeshwar Dayal,B.Sc. Satya Pal,M.V.Sc. –– Personnel –– Asstt. ChiefTechnicalOfficer Asstt. ChiefTechnicalOfficer Asstt. ChiefTechnicalOfficer Asstt. ChiefTechnicalOfficer Asstt. ChiefTechnicalOfficer Principal Scientist,Incharge Principal Scientist,Incharge Controller ofExaminations Joint Director(Academic) Sr. Scientist&Incharge Chief TechnicalOfficer Chief TechnicalOfficer Chief TechnicalOfficer Chief TechnicalOfficer Chief TechnicalOfficer Chief TechnicalOfficer Academic Coordinator Chief HostelWarden Sr. TechnicalOfficer Sr. TechnicalOfficer Sr. TechnicalOfficer Sr. TechnicalOfficer Sr. TechnicalOfficer Sr. TechnicalOfficer Sr. TechnicalOfficer Sr. TechnicalOfficer Principal Scientist Principal Scientist Principal Scientist Principal Scientist Technical Officer Technical Officer Technical Officer Technical Officer Technical Officer Technical Officer Hostel Warden Hostel Warden Hostel Warden Sr. Scientist Sr. Scientist Sr. Scientist Sr. Scientist Sr. Scientist Sr. Scientist Sr. Scientist Sr. Scientist Scientist Scientist Scientist Ganpat Rai,ITI Dharambir, B.A. S. K.Talwar,M.A.,P.G.D. G. D.Joshi,M.A. Gopal Sankhala,Ph.D. Communication Centre Jai Pal,BA,PGDCA Naresh KumarDahiya,M.Tech. Gian Singh,M.Sc. Anirudha KumarM.Sc.,PGDCA A. K.Sharma,Ph.D. Computer Centre B. P.Singh,M.A.,M.Lib.&Info.Sci. B. R.Yadav,Ph.D. Library Services Braj Kishor,M.A.,B.LibSci.&MSS Meena Malik,M.Phil.,Ph.D. G. R.Patil,Ph.D. Evaluation Cell Research Prioritization,Monitoringand M. P.Sharma Lakhvinder Singh Sarvan Kumar Ranbir Singh Balbir Singh Naresh Kanyana(ITIElectrical) Khem Chand(ITIElectrical) Sohan Lal,M.A.(Eco.)(ITIElectrical) Raj Kumar(ITIElectrical) Devinder Kumar(ITIWireman) Tek Chand(ITIElectrical) S. K.Saini Sarwan Kumar Pankaj Chawala,M.Tech.(Electrical) R. K.Bansal,B.E.(Civil) Incharge R. M.Chayal,Dip.CivilEngg. Maintenance Section Rajvir Singh,M.A.,PGDCA Deepak Chopra,B.A. Ashutosh, Ph.D. Security Section Kanchan Choudhary,M.A. (Off. Lang.) Ram ShankarGautam,M.A.P.G.D.T. Official LanguageUnit Ram Chander R. K.Mittal,B.E.(Mechanical) Vehicle MaintenanceSection Sarita Yadav,B.P.T.,PGDPMIR Latha Sabikhi,Ph.D. A. K.Singh,Ph.D. Ritu Chakravarty,M.Sc. Bimlesh Mann,Ph.D. Assistant ChiefTechnicalOfficer Asstt. ChiefTechnicalOfficer Asstt. ChiefTechnicalOfficer Joint Director(Research) Associate Prof.(English) (Chief TechnicalOfficer) Chief TechnicalOfficer, Chief TechnicalOfficer Chief TechnicalOfficer Sr. TechnicalOfficer Sr. TechnicalOfficer Sr. TechnicalOfficer Sr. TechnicalOfficer Sr. TechnicalOfficer Sr. TechnicalOfficer Security Supervisor Sports Coordinator I/c PlacementCell Technical Officer Technical Officer Technical Officer Technical Officer Technical Officer Technical Officer Technical Officer Technical Officer Technical Officer Technical Officer Technical Officer Technical Officer Technical Officer Technical Officer Technical Officer Technical Officer Technical Officer Deputy Director Hostel Warden Hostel Warden Admn. Officer Incharge Incharge Incharge Incharge Incharge P. M.Meena Prem Singh,M.A. Incharge Estate Section R. K.Malik,Ph.D. Health Complex R. S.Dhull Vinod Kumar D. Sherpa Hospitality Cell Manjunatha M.,Ph.D. S. Subhash,Ph.D. S. Varalakshmi,M.V.Sc. F. MagdalineEljeevaEmerald,ME(Agri.) S. Jeyakumar,Ph.D. M. Sivaram,Ph.D. Chitranayak, M.Tech. Mukund AmritraoKataktalware,Ph.D. Rekha RavindraMenon,M.Tech. P. HeartwinAmalaDhas,Ph.D. M. C.ArunmozhiDevi,Ph.D. D. N.Das,Ph.D. K. JayarajaRao,Ph.D. B. Srinivas,Ph.D. K. P.Ramesha,Ph.D. P. K.Dixit,Ph.D. B. V.Balasubramanyam,Ph.D. B. C.Ghosh,Ph.D. B. SurendraNath,Ph.D. C. N.Pagote,Ph.D. Satish Kulkarni,Ph.D. Southern RegionalStation,Bangalore Sushil KumarKamboj,M.Sc. Shish PalGupta & MidWifery Saroj Kathuria,DiplomaNursing Richa Walia,DiplomaNursing Manoj Kumar,M.B.B.S. Rekha Sharma,M.B.B.S. Inauguration of the Blood Donation Camp by Dr. Vandana Bhatia, Chief Medical Officer, Karnal Principal Scientist,Incharge Chief TechnicalOfficer, Chief MedicalOfficer Chief MedicalOfficer Sr. TechnicalOfficer Sr. TechnicalOfficer Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Technical Officer Technical Officer Technical Officer Technical Officer Technical Officer Technical Officer Sr. Scientist Sr. Scientist Sr. Scientist Sr. Scientist Sr. Scientist Sr. Scientist Sr. Scientist Scientist Scientist Scientist Scientist Head

H. C.Yadav,M.Tech. Prabir Saha,M.Sc. Somnath Dutta,M.V.Sc. Amitava Ghosh,M.V.Sc. Alokesh Goswami,M.Sc. Ajoy Mandal,Ph.D. M. K.Ghosh,Ph.D. Anupam Chatterjee,Ph.D. S. K.Das,Ph.D. Lotan Singh,Ph.D. R. A.Dey,Ph.D. P. K.Roy,Ph.D. T. K.Datta,Ph.D. Eastern RegionalStation,Kalyani Vimala, B.Sc. A. Louis,B.Sc. K. Ningaraju,M.V.Sc. Sreekanta Janakshi, M.C.A. K. GeethaKumari B. R.Srinivasamurthy,B.Sc. M. S.Nagarajaiah,Dip.(CivilEngg.) Meganathan, Dip.(Elect.Engg.) K. P.LakshminarayanappaDME(Mech.) Gurunath GoudaPatil,B.Sc. R. Keshvamurthy,B.Sc. P. G.Satisha,B.V.Sc. Thivija Kumari,M.A. K. L.Sampath,B.Sc. J. NageshwaraRao,Dip.(Mech) Veeraju, B.E.(Civil) B. K.Rajeshekaraiah,B.Sc. M. K.Vedavathi,B.Sc. M.A. Usha,B.Sc. P. Aravindakshan,M.Sc L. KrishnaMurthy,M.Sc. M. Sreenath,M.Sc. V.R.V. SurendranathNaik,M.D. M. Nanjundaswamy,Lib.Sci. P. Murugananthan,M.Lib.Sci. Asstt. ChiefTechnicalOfficer Asstt. ChiefTechnicalOfficer Asstt. ChiefTechnicalOfficer Asstt. ChiefTechnicalOfficer Asstt. ChiefTechnicalOfficer Asstt. ChiefTechnicalOfficer Asstt. ChiefTechnicalOfficer Chief TechnicalOfficer Chief TechnicalOfficer Chief TechnicalOfficer Chief TechnicalOfficer Chief TechnicalOfficer Chief TechnicalOfficer Chief MedicalOfficer Sr. TechnicalOfficer Sr. TechnicalOfficer Sr. TechnicalOfficer Sr. TechnicalOfficer Sr. TechnicalOfficer Sr. TechnicalOfficer Sr. TechnicalOfficer Principal Scientist Technical Officer Technical Officer Technical Officer Technical Officer Technical Officer Technical Officer Technical Officer Technical Officer Technical Officer Technical Officer Sr. Scientist Sr. Scientist Sr. Scientist Sr. Scientist Sr. Scientist Sr. Scientist –– Personnel –– Head 145 ndri annual report 2013-14 146 ndri annual report 2013-14 • • Promotions • • • • • • • • • • • • • • • • Joining

w.e.f. 1.1.2009. Bangalaore waspromoted toPrincipalScientist SRS, (AN) Scientist Sr Srinivas, Bandla Dr. w.e.f 1.1.2009. Bangalaore waspromoted toPrincipalScientist SRS, (AGB) Scientist Sr. Rao, Jayaraj K. Dr. Science University,Bidaron31.07.2013. Karnataka Veterinary,Animal and Fisheries on 01.08.2013aftergettingrelievedfrom SRS at joined (F/FT) T-6 Siddaramanna, Dr. w.e.f. 03.01.2014. as Head,DairyChemistryatNDRI,Karnal Dr. Bimlesh Kumari, Principal Scientist joined Division atNDRI,Karnalw.e.f.27.12.2013. Head (DM)joinedasHead,DairyMicrobiology Acting and Scientist Principal Malik, K. R. Dr. NDRI, Karnalw.e.f.11.09.2013. at joined (ASPE) Scientist Sr. Barnwal, P. Dr. at ERS,Kalyaniw.e.f.24.08.2013. Dr. Champak Bhakat, Sr. Scientist (LPM) joined at ERS,NDRI,Kalyaniw.e.f.08.08.2013. Dr. M. Karunakaran, Sr. Scientist (ARG) joined joined atSRS,NDRI,Bangalorew.e.f.01.08.2013. Science) (Animal Scientist Sr. Sivaram, M. Dr. joined atNDRI,Karnalw.e.f.22.07.2013. Dr. H. R. Meena, Sr. Scientist (Vety. Extension) joined atNDRI,Karnalw.e.f.25.6.2013. (Agronomy) Scientist Sr. Kumar, Rakesh Dr. joined atSRSw.e.f.11.6.2013. & Instrumentation)fromCIRCOT,Mumbai (Electronics Scientist Sr. Nayak, Chitra Dr. Division joined at NDRI,Karnalw.e.f.31.5.2013. Division joinedat Extension Dairy Head, Ponnusamy, K. Dr. from CIFT,Kochi. at NDRI,Karnalw.e.f.20.4.2013onhistransfer joined (ABT) Scientist Sr. Kumar, Rakesh Dr. ERS ofNDRI,Kalyaniw.e.f.16.4.2013. Dr. Mohan Mondal, Sr. Scientist (AP) joined at at NDRI,Karnalw.e.f.11.4.2013. Dr. Shaik Abdul Hussain, Scientist (DT) joined (LPM) joined at NDRI, Karnal w.e.f. 10.4.2013. Scientist Baithalu, Kumari Rubina (Ms.) Dr. NDRI, Kalyaniw.e.f.8.4.2013. Dr. Ashok Santra, Sr. Scientist joined at ERS of P ersonnel M • • • • • • • • • • • • • • • • • ilestones

25.02.2013. promoted as Sr. Technical Officer (F/FT) w.e.f. (F/FT) Officer Technical Patil, P. G. G. Sh. w.e.f. 13.01.2013. (F/FT) Officer Technical Sr. as promoted FT) (F/ Officer Technical Murthy, Keshava R. Sh. (F/FT) w.e.f.01.01.2013. promoted as Assistant Chief Technical Officer (F/FT) Officer Technical Sr. Chand, Tara Sh. Officer (F/FT)w.e.f.01.01.2013. Technical Chief as promoted (F/FT) Officer Technical Chief Assistant Chand, Ramesh Sh. Secretary w.e.f.17.08.2013 Private as promoted was PA Ranjna, Mrs. respectively. (Information) w.e.f.03.02.2000&03.02.2005, promoted asT-6(Information)&T-7/8 (Information) T-7/8 Ex. Kaur, Bhpinder Smt. T-6 (F/FT)w.e.f.01.01.2010. Sh. Deshwirt Sharma, T-5 (F/FT) promoted as (F/FT) w.e.f.01.01.2010. T-6 as promoted (F/FT) T-5 Singh, Prem Sh. (F/FT) w.e.f.10.01.2011. Sh. Sanjiv Kumar, T-5 (F/FT) promoted as T-6 FT) w.e.f.09.07.2009. (F/ T-5 as promoted (F/FT) T-4 Deepa, Mrs. promoted asScientist(SS)w.e.f.8.1.2011. Scientist Kalyani ERS, Mandal, Mohan Dr. w.e.f. 15.12.2012. ERS, KalyanipromotedtoPrincipalScientist (LPM) Scientist Senior Bhakat Champak Dr. grade w.e.f.4.7.2011. (SS) SRS, Bangalaore promoted to nexthigher Scientist Emerald, Eljeeva Magdaline F. Ms. 22.2.2011. Bangalaore promotedtoSeniorScientistw.e.f. Dr. Menon Rekha Ravindra, Scientist (SS) SRS, w.e.f. 02.09.2009. Bangalaore waspromotedtoSeniorScientist Dr. P. Heartwin Amaladhas, Scientist (SS) SRS, 16.08.2009. was promoted to Senior Scientist w.e.f. Dr. S. Jeyakumar, Scientist (SS) SRS, Bangalaore w.e.f 22-3-2012. Bangalaore waspromoted toPrincipalScientist SRS, (AGB) Scientist Sr. Das, N. D. Dr. • • • • • • • • • • • • • • • • • • • • •

with effectfrom02.09.2013. in thePB-4Rs.37400-67400+ RGPofRs.9000/- Scientist, Senior Amaladhas, Heartwin P. Dr. from 26.02.2013. Rs.15600-39000+ RGPofRs.8000/- witheffect PB-III Senior Scientistinthe to thepost promoted Scientist, Kataktalware, A. M. Dr. 10.02.2013. 39000 +RGPofRs.7000/-witheffectfrom Dr. Manjunath, Scientist, in the PB-III Rs.15600- from 16.08.2012. Rs.37400-67400+ RGPofRs.9000/-witheffect PB-4 the in Scientist, Senior Jeyakumar, S. Dr. Reproduction) (Animal Scientist Sr. Kumaresan, A. Dr. Dr. KaushikKhamrui,Sr.Scientist(DT) Dr. SenthilKumar,Scientist(D.Extension) Dr. SudarshanKumar,Scientist (ABTC) Dr. ManojKumarSingh,Scientist(ABTC) Dr. RishikantaSingh,Scientist(DESM) Advancement Scheme: to thenexthighergradeunderCareer promoted been have scientists following The 11.07.2013. w.e.f. (DM) Scientist Sr. Ram, Chand Dr. w.e.f. 01.09.2012. (LPM) Scientist Sr. Kamboj, Lal Madan Dr. 23.04.2012. w.e.f. (DC) Scientist Sr. Kumar, Rajesh Dr. 12.04.2012. Sharma, Vivek Dr. 12.04.2012. Sharma, Rajan Dr. 01.01.2011. w.e.f. (Biotech.) Scientist Sr. Viz, Shipla Dr. Principal Scientists: as promoted were Scientists Sr. following The Hyderabad. undergo FOCARS-99trainingatNAARM, 31.12.2013 tojointhepostofScientistand on relieved (L/T) T-3 Solanki, Chandan Sh. 01.07.2013. promoted asTechnicalOfficer(L/T)w.e.f. (L/T) Assistant Technical Sr. Pal, Jai Sh. 01.07.2013. promoted as Sr. Technical Officer (F/FT) w.e.f. (F/FT) Officer Technical Kumar, Vinod Sh. Sr. Scientist(DC)w.e.f. Sr. Scientist (DC)w.e.f. • • • Demise • • • • • • • • • • • • • • • Retirements/Transfers

(DCN) NDRIKarnalexpired on26.02.2014. Scientist Principal Sirohi, Kumar Sunil Dr. NDRI, Kalyaniexpiredon16.10.2013. Dr. P. K. Roy, Principal Scientist (LPM), ERS of 26.09.2013. & ActingHead,SRSBangaloreexpiredon Scientist Principal Retd. Vijayalakshmi, S. Dr. w.e.f. 31.3.2014. T-7/8 (L/T) retiredfrom Council’s service Officer Technical Chief Assistant Sarwar, Sh. Council’s servicew.e.f.31.03.2014. Dr. B.R. Yadav, Acting Head (DCB) retired from w.e.f. 28.02.2014. fromCouncil’sservice retired ERS, Kalyani Sh. Bhagwat Singh, Technical Officer T-5 (W/S) Council’s servicew.e.f.31.01.2014. Mr. K. R. Kisku, AO, ERS, Kalyani retired from service w.e.f.31.01.2014. FT) Dairy Extension retired from Council’s Sh. N.S. Sirohi, Chief Technical Officer T-9 (F/ 31.01.2014. FT) ATICretiredfromCouncil’sservicew.e.f. (F/ T-5 Officer Technical Sharma, Rajpal Sh. retried fromCouncil’s service w.e.f. 31.12.2013. (F/FT) Officer Technical Singh, Nihal Sh. from Council’sservicew.e.f.31.12.2013. Sh. Rishi Pal, Sr. Technical Officer (L/T) retried retried fromCouncil’s service w.e.f. 31.10.2013. Sh. Shiv Balak Rai, Balak Shiv Sh. join asADG(AP&B),ICAR,Hqrs.,NewDelhi. to 21.9.2013 on relieved was Gandhi S. R. Dr. to ERS,NDRIKalyaniw.e.f.26.08.2013. transferred fromNDRI, Karnal Extension) was (Dairy Scientist, Garai, Sanchita (Mrs.) Dr. 08.08.2013. retirement fromCouncil’sservicew.e.f. voluntary took (DT) Head Patel, A. A. Dr. service on26.06.2013. (FM) retired onsuperannuationfrom Council’s Scientist/Incharge Principal Harika, S. A. Dr. service on29.06.2013. retired onsuperannuationfromCouncil’s Dr. R. A. Dey, Senior Scientist (Agri. Extension) 30.04.2013. on superannuationfromCouncil’sservice (L/T) and Mr. S. C. Biswas, T-5 (L/T) retired T.L. Jaggi,T-5 (F/FT), Sh.Balbir Singh,T-5 Sh. Scientist, Principal Sachdeva, K. G. Dr.

Technical Officer (W/S) Officer Technical –– Personnel Milestones –– 147 ndri annual report 2013-14 148 ndri annual report 2013-14 MAINsTATION 15 the Institute. However, separate faculty of LPM carved out of Dairy Cattle Breeding Division of in June,2009afterbeing section cameintobeing The Livestock Production andManagement (LPM) (LPM) Livestock ProductionManagementSection Cattle BreedingDivision. integral partoftheresearchcomponentDairy Tharparkar) andMurrahbuffaloesisalsothe (Karan Fries,Karan Swiss,Sahiwaland of cattle Research Complex.Besidesthis,breedingherds Livestock Record Cell and Artificial Breeding Genetics Lab.,LivestockGenomicAnalysis Genetics Lab.,SireEvaluationMolecular The other stakeholders. developmental agencies,stategovernmentsand semen and breeding malestofarmers, livestock supplies superior germplasm intheform of frozen services tofarmersandvariousorganizations, TBI (SINED)intrainingprograms,consultancy Division. TheDivisionalsoassistsKVK/DTCand organized undertheaegisofCAFT (AG&B) inthe 30 NationalTrainingPrograms have so far been areas ofAnimalGeneticsandBreeding.Atotal Livestock DevelopmentOrganizationsinadvanced State Agricultural/VeterinaryUniversitiesand scientists/teachers from Research Institutes, research, teachingandimpartingtrainingto plan continueditsactivitiesonstrengthening Division of Dairy CattleBreeding during eighth Animalin GeneticsandBreeding established at The Center of Advanced Faculty Training(CAFT) genetic disordersandassessment of malefertility. and thermo-tolerance,screeningofyoungmalesfor production/reproduction traits,diseaseresistance molecular levelanditsassociation with studies at plans, geneticpolymorphism optimum breeding evolving selection criteria, development of faster multiplicationofsuperiorgermplasm, males andjudiciousselectionoffemales, Murrah buffaloes by progenytestingof breeding improvement ofcrossbredandSahiwalcattle The mainthrustresearchareasaregenetic related tomolecular genetics. and biotechnology genetics andbreedingincluding cytogenetics conducting researchintheareasofanimal The Divisionhasbeenactivelyinvolvedin Dairy CattleBreedingDivision(DCB) Division has Animal Breeding Lab., Biometrical Division has Animal Breeding Lab., Biometrical R esearch D fine instrumentation laboratory (2) laboratory for laboratory (2) laboratory instrumentation fine include(1)centralto time.Thecentralfacilities organizations seekingsuch supportfromtime not onlyfortheInstitutebut also for various sister are centralfacilitiesforresearch andeducation has builtupexcellentlaboratory facilities,which for chemicalandphysicalanalysis.TheDivision are equippedwithmodern analytical instruments in therespectiveaspects.Theresearchlaboratories States ofAmericatogainexpertiseandexposure in Europeancountries,CanadaandUnited repute areas and have visited advanced institutions of have beenadequatelytrainedinvarious priority occupying different positions. The faculty members team of highly qualified and experienced scientists forage production.TheDivisionhasawell-knit refinement in the discipline of animal nutrition and and fieldleveltechnologydevelopment and education throughvarious training programmes and participatesintheprocessofextension research, post-graduateprogrammesofeducation The Divisionundertakesbasicandapplied Dairy CattleNutritionDivision(DCN) dairy farms. ofcommercial forestablishment and entrepreneurs and to provide consultancy to theneedyfarmers of-the-art dairy animal production system to clients management practicesandtodemonstratestate- of package the standardize (iv)to production; divisions, intheupstreamareasofdairyanimal to carryoutresearchincollaborationwithdifferent infrastructure for high yieldingdairy animals; (iii) dairy animalmanagementfacilitiesand of-the-art state- of development (ii) breeds; identified the elite germplasm repository of dairy animals of The objectivesofthesectionare:(i)tomaintain training and extension activities of the Institute. Research Center of Institute besides supporting the bullsmaintainedattheAnimal Breeding breeding of theroutinemanagementcattleyardand LPM faculty isalsoshoulderingtheresponsibility engaged inteachingbothatUGandPGlevels.The management of dairy animals. The faculty of LPM is of packagepracticesontheroutinecareand technologies anddevelopment many transferable production andhasbeensuccessfulinevolving frontline areasofallappliedaspectsdairyanimal of LPM is engagedinconductingresearch in the programmes in LPM were continuing.The faculty 1976 and post graduate and doctorate degree alongwith BoardofStudieswasinexistencesince ivisions methane analysisequipmentusingSF Nutrition Divisiontilltheendof6 Division and subsequently of Dairy Cattle as asectionoferstwhileDairyHusbandry functioned The disciplineofAnimalPhysiology Dairy CattlePhysiologyDivision(DCP) nutrient utilizationand to observe the studyof a particular gene/locion interaction studiesarefullswinginthedivision few years precision nutrition and nutrientgene spectrophotometer, PCRmachineetc.Forthelast chromatography, HPLCsystem, atomic absorption spectrophotometer, gas-liquid available includeGC-massspectrophotometer, Some ofthesophisticatedinstruments laboratory. feed processingunit(6)nutritionalbiotechnology methanogenesis (4) qualitycontrollaboratory(5) for environmentrelatedstudiesincluding anaerobic rumen microbial work (3)laboratory counts in milk of bovines during different stages counts inmilkofbovinesduring residual milkandsecretion rates;somaticcell ofuddercapacities,administration, estimates augmentation oflactationthrough growthhormone of feeding andmilking; induction oflactation, and fertilityimprovement, optimum frequency of endocrinetechniquesforgrowthenhancement growth, reproduction and lactation; application interrelationships duringdifferentphasesof transfer; neonatephysiology;temporalendocrine parturition induction, superovulation andembryo and their applicationinhormone immunoassays; IgG (second antiserum andanti-rabbit antibody) indigenousproductionofprogesterone viability; fetal and pregnancy of confirmation for milk in application ofestronesulphatedetermination disorders, ovarian of identification and cyclicity pregnancy/non pregnancydetection,monitoring confirmation, estrus viz. improvement fertility for fluids body in measurement progesterone of application PGFM; and estradiol-17β prolactin, oxytocin, GH, LH, FSH, cortisol, testosterone, sensitive enzyme immunoassays for progesterone, steroid hormones; development and validation of radio-immunoassays forseveralproteinand (THI) mapsofIndia,developmentsensitive development oftemperaturehumidityindex notable salientachievementsofthedivision are: The technologies. level field of application and at Master’s and Doctoral levels and Development programmes ofeducationinAnimalPhysiology Animal Physiology;(ii)toundertakepostgraduate in variousaspectsof research andimparttraining division are:(i)toconductbasicandapplied an independentDivision.Theobjectivesofthe Plan. Intheyear1984,itreceivedstatusof vice versa. 15 N- Analyzer, 6 th technique, FiveYear Deemed University. programmes inBiotechnology fortheNDRI Processing and3)Toorganize M.Sc.andPh.D. of Biotechnology inDairyProduction and Dairy dairy products,2)Totrainmanpower inapplication safe andwholesome producing superiorquality, and for developing innovativedairy processes for programmes forimprovinganimalproductivity biotechnology orientedbasicandappliedresearch of theproposedDivisionare1)Toundertake Biotechnology) programmes.Theobjectives offers M.Sc./M.V.Sc./M.Tech and Ph.D.(Animal in dairy production and processing, the Centre also Besides research onareas relevanttobiotechnology created under various programs on biotechnology. by consolidatingalltheinfrastructurefacilities Biotechnology CentrewasreorganizedinJune1999 National AgriculturalResearchProject-II.Animal Genome LabandMolecularBiologyUnitunder strengthened byestablishmentofLivestock of Biotechnology. Biotechnology was further by financialsupportfromtheDepartment state-of-art EmbryoBiotechnologyCentre(EBC) ofa fortheestablishment formed thebasis improving animalproductivityinourcountry superior females of dairy breeds of ruminants for advances inreproductionandproductionto biotechnological ofrecent need forapplication Excellence onBiotechnology’program.Theurgent during mideightiesunderaUNDP‘Centreof Biotechnology wasinitiatedatNDRI,Karnal Animal BiotechnologyCentre(ABTC) on climateresilientlivestock. CIRB, HisarandSAU’s research for strengthening with otherICARInstitutesviz.IVRI,Izatnagarand established forcollaborativeresearchventures livestock wastakenup.Linkageshavealsobeen agriculture toclimatechangewithemphasison on impact, adaptation and vulnerability of Indian Project livestock productionsystem.ANetwork unique traitsincattleandbuffaloesforresilient going for basic and strategic research to identifying Climate ResilientAgriculture(NICRA)hasbeen NDDB andDST-DAAD. NationalInitiativeon BARC, World Bank, Department of Biotechnology, also undertakenresearchprojectsfundedbythe and Environmental Physiology. The Division has Reproductive Physiology, Lactation Physiology and main areasisnamelyGrowth in three thrust cycle forworkingbullocks.Presently,itsresearch and buffalobullocksoptimumwork-rest efficiency of crossbred and indigenous bovine of lactation;draughtevaluationsystem,work –– Research Divisions –– 149 ndri annual report 2013-14 150 ndri annual report 2013-14 Characterization of 8 amino acid transport systems Characterization of 8 amino acid transport systems and itsusefordetectionof sub-clinicalmastitis; Characterization and sequencing ofmilk lysozyme of conjugatedlinoleicacid anditsmechanism; linoleic acidinmilkproducts; Bodyslimmingeffect minerals fromdairyproducts.Levelsofconjugated mechanism; Bioavailabilityofvitaminsand of immunesystem,andelucidationitsmolecular intestinal andmammary cancerand improvement of dairygheeincoronaryheartdiseases, gastro- of diabetes;Validationnutraceuticalattributes against gastrointestinalcancer and management levels,protection serumcholesterol reducing dahi andvalidationoftheirhealth-benefitsin are: DevelopmentoffivetypesProbiotic The salientresearchachievementsoftheDivision facilities arealsoavailableintheDivision. out researchatlowtemperature.Cellculture incubators. Divisionhasacoldroomforcarrying low temperaturefreezersandCarbondioxide (Imaging) systems, ultra filtration unit, Ultra- machines, Freezedryer, Geldocumentation flaking Ice microscopes, fluorescent and Inverted Chromatography, GasLiquid PCR, ELISAPlatereaders,HighPressureLiquid Spectrofluorometer, spectrophotometers, Visible High speedcentrifuges,Micro-centrifuges,UV- viz. Alpha-andBeta-counters,Ultra-centrifuge, instrument rooms withmodern equipments processing andproduction.TheDivisionhas stem cellsresearchandBioinformaticsindairy cryopreservation of semen; Spermatogonial of buffalofertilitygenes;Spermfunctionsand dairy products;Nutrigenomics;Characterization Validation ofhealthbenefitclaimsIndian nutraceuticals andtheirmechanismofaction; towards Developmentofprobioticfoods;Dairy endeavors oftheDivisionarepresentlydirected Human NutritionandDietetics.Research Dairy ChemistryDivisionandthesectionof of thedisciplineAnimalBiochemistry into being in September 1984 with themerger The DivisionofAnimalBiochemistrycame Animal BiochemistryDivision(ABC) Proteomics Research,StructuralBiology,etc. Regenerative Biotechnology,AnimalGenomics, specialized laboratoriesonEmbryoBiotechnology, 20,000 sq.ft.andhas space ofmorethan working research atNDRI.Thenewlabfacilityoffersa biotechnology created in2007toconsolidatethe of-the-art biotechnologyresearchfacilitywas Genomics LabandProteomicsLab.Astate- Centre areEmbryoBiotechnologyLab,Animal The three main labs at theAnimal Biotechnology –– Research Divisions –– simple tests for the detection of adulteration of ofadulteration; offatfordetection availability test for simultaneous estimation of milk fat and of IndianStandards;modification ofGerber under theMinistryofHealth aswellBureau the CentralCommitteeofFoodStandards by nowprescribed of milkandproducts standards of SNFinmilk;formulationquality rapid andsimplemethodsforthedetermination minerals and trace elements and theirpartitioning; of variousprocessingparametersonthemajor influence membrane; globule fat milk of integrity andstructural residue;chemicalmakeup ghee of properties antioxidant flavour; residue ghee dairy products;revealingchemistry of ghee and evaluating roleofphospholipidsinstability milk fat; of buffalo milk andglyceridestructure milk andrasogolla;humanizationofbuffalo satisfactory productslikecheese,condensed and micellarstabilityenablingmanufactureof and alterationinitscalciumcaseinlevels of physico-chemical properties ofbuffalo milk products. The salientachievementsare:evaluation knowledge onthechemistryofmilkand industry. The Division has contributed significant quality controlrelatedproblemsofthedairy and toprovideR&Dsupporttowardschemical- for undergraduate and postgraduate courses products, toimparteducationalprogrammes understanding chemistryofmilkand fundamental and applied research for The mandateoftheDivisionistoconduct Dairy Chemistry(DC) hamster, etc. and for laboratoryanimalsviz.rat,mouse, rabbit that caterstotheneedofstudentsandscientists Sensor. TheDivisionmanagesSmallAnimalHouse Endocrinology, Reproductive Biochemistry and and Immunology;Bioinformatics;Molecular Prebiotics andProbiotics;Bioactive Peptides Nutregenomics, functional foods and therapeutics; The Division has thelaboratories for steroidogenesis andapoptosis. granulosa cells during (PI3K andMAPK)incattle in anestrusbuffaloes;Transductionpathways SSCP analysisofCYP19aromatase gene buffalo; regulation offertilityrelatedovariangenesin Expressionand hormonalof spermfunction; semen freezing;Signaltransductionmechanism maturation, capacitation,acrosomereactionand phases ofbuffalo;Biochemical changes insperm reproductive of profile Hormonal mechanism; its of lactation; Antiatherogenic properties ofmilk and in mammary gland and their induction at the onset of vitamin D An analyticalprotocoldevelopedfortheestimation oil detectioningheeusingRP-HPTLC; vegetable and neutralizersinmilk;Methoddevelopedfor ofaddedurea developedforthedetection tests testing ofmilk and milk products: Stripbased developedforDifferent analyticalmethods exchange andconcentration. milk; amulti-purposedevice for dialysis; buffer to detect adulteration of milk with 2.5 to 10% soya detection ofdetergentinmilk;acolourbasedtest and lowcholesterolghee;aplatformtestfor calcium fortified milk, coconut filled Gouda cheese for non-dairywhitener,lowfatfrozendessert, residues inmilkandproducts;methodologies on vitamins;statusofpesticidesandantibiotic dairy products; effect of processing treatments antioxidants inenhancingtheshelflifeoffatrich of WPC;renovationrancidgheeandrole lactoperoxidase;functional properties of buffalo of lactoferrin;characterizationandcrystallization role andbacteriostatic sources;structure vegetable and calves fistulated from rennet of preparation of butterandcheesecolourfromannattoseeds; ofmanufacturing methods milk andproducts; laboratory and e-Learning laboratory. The Division laboratory ande-Learning laboratory. The Division food technologylaboratory, dairyby-products foodlaboratory, infant laboratory, milks fermented indigenous milkproductslaboratory, cheeseand evaluation laboratory,packaging laboratory, got eightwell-equippedlaboratoriesviz.,sensory product manufacture. AtpresenttheDivision has activities intheareaofdairyprocessingand and applied research, training and consultancy Institute, Karnal.Itisinvolved in teaching,basic Research Dairy National at up set Divisions first The DairyTechnologyDivisionisoneofthe Dairy TechnologyDivision(DT) neutraceuticals andqualityassurance). and bioactivepeptides,minerals,functionalfoods/ Instrument room,Researchlabs(lipids,proteins labs, UndergraduateandPostgraduate lab, control the-art SeminarroomandLectureroom,Quality animal modelsystem.TheDivisionhasstate-of- proteins and assessed for antioxidant activityin of curcuminnanoemulsionoptimizedusingmilk their antioxidantactivity.Process for preparation 207). Buffalo α Buffalo 207). (24-34), β-CN (197-209), β-CN (199-209), β-CN (193- proteolytic from skimmilkbyfermentationusingselected body fat in ghee. Antimicrobial peptides identified assessed for detection of soybean oil and buffalo physico-chemical propertiesdifferentmethods strains including α including strains lactobacillus 2 in fortified toned milk and based on s -casein hydrolysatesassessed for S1 -CN a dairy industryandresearchorganizations. Besides, NCDC caterstotherequirement forculturesfrom a singularfacilityofitskind inthecountry.The National CollectionofDairyCultures(NCDC)is Organisms/Products and Rumen Microbiology. Health, Biopreservatives, Ultrastructure ofDairy Bioactive peptides, Quality Assurance and Public Milk Products,Probiotics,prebioticsand Engineering, StarterCulturesand Fermented areas related to Molecular Biology and Genetic Divisioncoversthe ofthe the researchwork Dairy MicrobiologyandBiotechnology.Broadly, research andteachingactivitiesinthefieldof The Division is currently engaged in handling universities/colleges. dairy industrypersonnelandstudentsfromother short termspecializedtrainingprogrammesfor analysis of milk and milk products and organizes as supplyofstarterculturesandmicrobiological offers contractual and consultancy services such and postgraduatelevelcourses.TheDivision educational programmes for both under graduate development ofhumanresourceandimparting DM facultyhas beenextensivelyinvolvedin as appliedaspectsofDairyMicrobiology.The well as basic both in significantly contributing The DivisionofDairyMicrobiologyhasbeen Dairy MicrobiologyDivision(DM) cookers, etc.arealsoavailableintheDivision. membrane processingunits,rotaryoven,extrusion cream manufacturingunit,HTSTmilkpasteuizer, and spraydryer.Pilot-scalevacuumdryer,ice cream-manufacturing unit, multistageevaporator ice equipments, manufacturing andghee butter and fermentedproductmanufacturingunits, cheese homogenizer, pasteurizer, like equipments products. Thedairyisequippedwithallmodern liters ofmilkintoadiverserangedairy Experimental Dairycapableofprocessing 10,000 the pilot/semi-commercialscaleisawellequipped concepts to laboratory work forscalingupofthe available totheDivision for undertaking R & D dairy. Another unique feature of the infrastructure necessary forundertakingcuttingedgeresearchin whichare MAPanalyseretc., osmosis units, packaging system,ultrafiltrationandreverse Soxhlet extractor,spectrophotometer,vacuum automatic units, flow air laminar analyser, visco dryer, water activity meter, viscometer, rapid colour meter,highspeedhomogenizer,freeze e.g., micro-fluidizer, food texture analyzer, instruments ofadvancedanalytical houses arange under NAIPprojectnoC4/418001-04 iscurrently

referral center withfunding (19.62crores) –– Research Divisions –– 151 ndri annual report 2013-14 152 ndri annual report 2013-14 economics of milk production and processing, with economics ofmilkproduction andprocessing,with in theDivisionwasonconducting researchin Year Plan.Intheearlystage, thefocusofresearch Management was created during the IV Five The Division of Dairy Economics, Statistics and (DES&M) Dairy Economics Statistics &Management during summer. specialized trainingfor thegraduate engineers in thedairyindustry.TheDivisionalsoconducts for applicationofmachinevisiontechnologies was established to initiatefrontier research and rabri and ghee. Recently, a machine vision lab burfi, basundi, khoa, of manufacture continuous SSHE is fully automatic state-of-the-artsystem for of gajarpakusingthreestageSSHE.Three rabri usingin-linesystem,coninousmanufacture machine, processforacceleratedproductionof of scaledupdesigncontinuouskhoamaking Recent research achievements include preparation separator attachmentforsmallscaleapplications. industrial scaled upandpaneergadgetcream khoa, paneer,rasgolla,malailaccha,etc.onthe developed prototypesforthemanufactureof of thedevelopedequipments.TheDivisionhas their collaborationindevelopment or inadoption ups withequipmentmanufacturersandusers for TheDivisionhastie- manufacturers. equipment and effortsarebeingmadetotransferthemthe Many oftheseequipmentshavebeenpatented for manufacturingindigenousmilkproducts. in developinganumberofprocessequipment decades, the division has achieved breakthrough and teaching activities. Duringthepast three and Equipment testing hall to support both research laboratories, Research&DevelopmentWorkshop addition to this,therearepost-graduateteaching Design, UnitOperationsandInstrumentation.In such asProcess Engineering,Process Equipment programmes and areas specific of needs the to facilities tocater Division hasresearchlaboratory research, training and industrial consultancy.The of theInstitute.Itiscontributinginteaching, one ofthemajorresearchdivisionssinceinception asDairy EngineeringDivisionwasestablished Dairy EngineeringDivision(DE) bioactive peptidesandvalueaddeddairyproducts. focus of DMDivision is onprobiotics,food safety, present, themainstate-of-the-art facilities.At Unit havebeenrenovatedwith Molecular Biology country. addressing the scientific issue on milk safety in our under progress for itsestablishmentatNDRI –– Research Divisions –– The laboratories of the Division including The laboratoriesoftheDivisionincluding technologies amongfarming community. dissemination of dairy production and processing February, 2009,tostrengthen theeffective Door” isinitiatedin Farmers’ at Education A newExtensionEducation Programme “Dairy as easyavailabilityoffeedbackfromthefarmers. and betterdissemination of technologiesaswell helps inunderstandingtheproblemsoffarmers activities strengthenthelinkageswithend users, These regularly. villages adopted workshops atthe andfieldaid campaigns,KisanSanghosthies transfer campaigns,infertilityandveterinary future. TheDivisionalsoorganizestechnology problems, butalsotorealizethevisionfor today’s for solutions find to organizations dairy for dairydevelopment.Interfacenotonlyhelpsthe together convergence ofallstakeholdersworking the Divisiontoprovideanopportunityfor Industry-Farmer Interfaceis also organizedby are organized by the Division. Research-Extension- as Dairy Mela and demonstrations, field days, etc The mainextensionprogrammeoftheinstitutesuch post graduateanddoctoral programmes of NDRI. engaged in human resource development through of dairyinnovations.Thefacultyhasalsobeen technology developmentandimpactstudies behaviour, informationmanagement,participatory Communication Technologies,organizational the division are intheareasofInformationand ResearchendeavorsofExtension Education. activities, besidesteachingandresearchin NDRI, KarnalinMay,1961toundertakeextension Dairy ExtensionDivisionwasestablishedat Dairy ExtensionDivision(DairyExtn.) programmes ofthescholars. staff researchprojectsandpost-graduate and economicimpactassessmentthroughthe economic reformsondairysector,climatechange aspects ofvaluechainmanagement,implication the Divisionenvisagedtoworkonadvanced From simpleeconomicanalysisofmilkproduction, priorities and conducting theresearch accordingly. the Division has been orienting its research to theresearch demands of the clientelesystems, divisional library andcomputer unit. Inresponse of scientific manpower,teaching andtraining aids, years, has developed good infrastructure interms evaluation andtransfer.TheDivision,overthe forward linkage factors for facilitating technology dairy enterprise encompassing backward and and broader aspectsof enveloped moreintricate periods, the researchprogrammes of theDivision thrust oncost-returnsstudies.Duringsubsequent • • respectively. The milking average of Sahiwal cows day, per head per FCM 4% kg 13.2 and 12.7 was by theNDRIviz.KaranSwiss andKaranFries performance ofthetwocrossbredstrainsdeveloped current yearwas1343882.4kg.Theproduction The totalmilkproductionoftheherdduring Livestock ResearchCentre(LRC) Grand Total On BookValue Public Auction Mode ofDisposal Male Calves upto6months Total Bucks Kids upto6months Male Goats Yearling 6-12 months Kids upto6months Female Age Group Age group Female Heifers Cows Total Teaser Bullocks/ (young) Male stock day: per Milk in Animals of Number Average Total MilkProduction(kg) Figures inparenthesesdicatesthetotalnumberofanimals sold. Auction ofanimalswasconducted on20 Milk productionatNDRI,Karnalduringtheyear2013-14(01.04.2013to31.03.2014) Sahiwal Sale ofLivestockduringtheyear2013-14(01.04.2013to 31.03.2014) 19 18 128 132 305 08 - 818693.00(226) 600893.00(52) 217800.00(174) Cattle Bovine StrengthofCattleandBuffaloesason31.03.2014 Tharparkar Alpine xBeetal 109 10 08 45 46 Flock StrengthofGoatsason31.03.2014 - - 107 12 04 28 28 35 -- S Karan Swiss Goats Buffalo Cattle upport 758094.00(48) 481494.00(37) 276600.00(11) Buffaloes 01 01 12 19 33 Cattle - - th &21 S st Karan Fries kg (KS-4395),respectively. and 25.0 (KF-7238) KS crossbred cowswas34.0kg current year.ThepeakmilkyieldbytheKFand buffalo (MU- 490) was 19.0 kg perdayduringthe of 20.0kginpeaklactation.BestyieldMurrah Sahiwal cow(SW-1931)producedbestmilkyield (6% FCM) kg per animal per day, respectively. One 9.2 and FCM) (4% 7.7 was buffaloes Murrah and ections September, 2013and21 Sannen xBeetal 15 25 108 01 181 330 - 141380.00(55) 40180.00(25) 101200.00(30) Goats 35 04 01 06 16 08 -- 1343882.4 kg 32 101 236 Local 01 01 - - - - - Total 45 52 293 09 378 778 01 st &22 Buffaloes Murrah 14 23 108 15 170 330 1718167.00 (329) 1122567.00 (114) 595600.00(215) Total - nd March2014. Total 142 16 05 34 44 43 -- Total Bovines 1108 59 75 401 24 548 01 153 ndri annual report 2013-14 154 ndri annual report 2013-14 –– Livestock Research Centre –– April May June July August September October November December January February March Average Months Animal Number Best yield(kg)inaday Overall average(kg)perday Milking average(kg)perday Average numberofdryanimalsperday Particulars Particulars Animal Number Best yield(kg)inaday Overall average(kg)perday Milking average(kg)perday Average numberofdryanimalsperday Average numberofanimalsinmilkperday Average numberofanimalsinmilkperday Month-wise MilkingAverage(kg)ofCows,BuffaloesandGoatsMaintainedatNDRI,Karnal Performance ofDairyAnimalsduringtheyear2013-14(01.04.2013to31.03.2014) Sahiwal No of milk/ mals ani- day 73 75 78 79 79 75 64 61 62 63 63 68 70 Flock performanceofGoatsduringtheyear2013-14(01.04.2013-31.03.2014) in animal/ yield Milk (kg)/ day 7.1 7.8 7.2 6.8 6.3 6.8 7.3 7.4 7.0 6.8 6.9 6.5 7.0 No of milk/ mals ani- Tharparkar day 16 18 20 18 17 17 12 10 12 11 12 18 15 in (kg)/ yield Milk mal/ day ani- 5.5 5.8 6.1 5.8 5.0 4.9 6.4 6.7 5.5 6.4 6.8 6.1 5.9 2013-14 (01.04.2013to31.03.2014) Cows No of milk/ Karan Swiss mals ani- day 17 16 13 12 14 16 18 17 18 17 15 14 15 in SW-1931 Sahiwal 20.0 3.8 7.0 57 70 yield Milk (kg)/ mal/ day ani- 11.9 11.1 12.8 11.9 12.5 12.6 11.5 12.3 12.1 12.7 11.6 12.2 12.6 No of milk/ mals ani- day Karan Fries 145 142 139 137 143 137 126 123 123 128 140 152 136 in Tharparkar TP-1183 17.0 2.5 5.9 20 15 yield Milk (kg)/ Alpine xBeetal mal/ ani- 13.0 12.1 12.0 10.8 10.4 10.9 12.3 13.1 13.8 13.1 13.5 13.8 12.4 day AB-28 No of 3.1 1.0 1.3 06 25 milk/ mals ani- day 105 101 101 109 106 103 101 98 99 96 97 94 97 Buffaloes in Murrah Karan Swiss Genetic Groups KS-4395 yield Milk 25.0 8.6 12.6 (kg)/ /day ani- mal 07 15 7.6 7.8 7.3 7.0 7.2 7.1 7.6 7.2 7.4 7.2 7.5 7.7 7.3 Genetic Groups in milk/ animals No of Alpine xBeetal day 28 30 31 32 31 26 18 12 23 25 24 23 25 Karan Fries KF-7238 yield (kg)/ 34.0 9.2 12.4 48 136 animal/ Milk day Sannen xBeetal 1.5 1.4 1.4 1.2 1.0 0.8 0.6 0.8 1.3 1.5 1.6 1.7 1.3 SB-209 2.7 0.7 1.1 Goats 05 07 in milk/ animals Sannen xBeetal No of day 04 07 07 07 06 05 03 03 10 12 11 10 07 MU-490 Murrah 19.0 4.5 7.3 65 101 animal/ yield Milk (kg)/ day 1.2 0.8 1.0 0.9 0.9 0.7 0.6 0.9 1.1 1.5 1.3 1.3 1.1 April,2013 May,2013 June,2013 July,2013 Month August,2013 No. ofobservations Average fat% September,2013 Range October,2013 No. ofobservations Average SNF% November,2013 Range December,2013 January, 2014 February, 2014 March, 2014 Average Months April, 13 May June July Total March, 14 February,14 January, 14 December November October September August Month-wise MilkingAverage(kg)asFatCorrectedMilkofCowsandBuffaloesMaintainedat Particulars Fat andSNFPercentageofCattleBuffaloesduring theyear2013-2014 Fodder andConcentrateduringtheyear2013-14(01.04.2013to31.03.2014) in milk/ animals No. of day Av. Sahiwal 4%Fat 68 75 78 79 79 75 64 61 62 63 63 68 70 13209.25 6362.50 13438.75 15832.75 188771.25 22925.50 18992.00 19813.50 19935.25 10380.25 12070.25 15547.50 20263.75 Green yield (kg.)/ Animals / Animals FCM 7.59 8.46 8.06 7.51 7.00 7.17 8.37 8.08 7.86 7.96 7.03 7.10 7.68 day Av. NDRI, Karnal(April2013-March2014) nml in animals Av. No.of 184 4.68 3.50-5.82 184 8.73 7.93-9.24 milk/day Tharparkar 4%Fat 12 18 20 18 17 17 12 10 12 11 12 18 15 Dry/Hay Sahiwal 6032.86 238.15 845.00 843.15 729.05 220.46 260.25 370.75 320.00 1265.80 810.25 45.00 85.00 Type ofFodder(Qntls.) yield (kg.)/ animals/ FCM First Lactation 8.70 6.51 6.70 6.64 5.43 5.41 7.53 8.17 6.53 7.14 6.60 7.04 6.87 day Av. 61 4.75 3.90-6.12 61 8.74 8.53-9.10 Tharparkar in milk/ animals Karan Swiss4%Fat No. of 6865.50 1501.00 368.50 8816.00 day Av. Silage 14 16 13 12 14 16 18 17 18 17 15 14 15 81.00 ------Cows yield (kg.)/ animals/ FCM 12.33 11.59 12.65 12.25 13.93 12.98 12.54 12.78 12.98 13.89 11.54 12.65 12.68 day Av. Karan Swiss 47 4.23 3.56-5.10 47 8.71 8.52-8.97 n milk/ in animals 13510.71 13488.25 15310.50 23163.65 19837.00 20656.65 20664.30 11646.05 12880.50 15592.50 20348.75 16521.25 203620.11 Karan Fries4%Fat No. of G. Total day Av. 159 142 139 137 143 137 126 123 123 128 140 152 137 522 4.26 3.45-5.64 522 8.71 8.12-9.07 Karan Fries yield (kg.)/ animals/ FCM 14.37 13.17 12.28 11.65 11.09 11.21 13.02 13.64 14.67 14.47 13.52 14.95 13.17 day Av. –– Livestock Research Centre –– 308 7.97 5.66-10.24 308 9.80 9.23-10.84 n milk/ in animals No. of Concentrate 94700.00 107712.00 119888.00 94100.00 94650.00 116200.00 100500.00 117200.00 105400.00 106100.00 106317.00 107233.00 1270000.00 day Av. 105 101 109 106 103 100 Murrah 6%Fat 98 99 96 97 94 97 94 Buffaloes (kgs) Murrah yield (kg.)/ animals/ FCM 8.97 9.37 8.50 9.55 9.32 8.56 9.72 9.11 9.76 9.43 8.22 9.68 9.18 day Av. 155 ndri annual report 2013-14 156 ndri annual report 2013-14 Range Average SNF% No ofobservations Range Average fat% No. ofobservations Production andReproductionPerformanceofCattleInitiatingtheirLactationduring2012 Average numberoflactations Calving interval(days) Service period(days) Dry period(days) Range Lactation length(days) Average lactose 305 orlessdaysmilkyield(kg) No. ofobservations Range Total milkyield(kg) Calving interval(days) Average protein Service period(days) No. ofobservations Particulars Range Dry period(days) Average Lactose Lactation length(days) No. ofobservations Range 305 orlessdaysmilkyield(kg) Average protein Total milkyield(kg) Age at1stcalving(Months) First Lactation Traits No. ofobservations Particulars First Lactation Protein andLactosePercentageofCattleBuffaloesduringtheyear2013-2014 Particulars 4.05-5.78 2.68-3.96 4.28-5.54 2.68-3.76 Sahiwal Sahiwal 4.90 3.28 4.91 3.30 847 847 184 184 8.03-9.70 3.19-6.18 Sahiwal 41.08 (35) 1748 (83) 1769 (83) and Completing in2013 Sahiwal 3.44 (83) 143 (60) 131 (61) 130 (49) 266 (82) 2262 (8) 2465 (8) 103 (7) 152 (7) 344 (8) 8.75 4.57 847 847 60 (3) Tharparkar Tharparkar 4.47-5.77 2.79-3.88 4.50-5.66 2.86-3.77 4.91 3.28 4.92 3.28 All Lactations All Lactations 174 174 All Lactations 61 61 Tharparkar Tharparkar 8.51-9.10 3.56-6.15 1428 (16) 1459 (16) 1.81 (16) 48.72 (6) 254 (16) 1462 (6) 1536 (6) Cows Cows 388 (9) 107 (9) 162 (9) 374 (4) 179 (4) 251 (6) 90 (4) 8.73 4.66 174 174 Karan Swiss Karan Swiss Cows 4.18-5.77 2.77-3.86 4.63-5.15 2.91-3.64 4.87 3.23 4.89 3.23 175 175 47 47 Karan Swiss Karan Swiss 8.47-9.01 3.52-5.49 3919 (19) 4197 (18) 2.52 (19) 426 (13) 137 (13) 331 (18) 3702 (6) 4492 (6) 3591 (6) 89 (13) 507 (2) 215 (2) 382 (6) 75 (2) 8.72 4.22 175 175 Karan Fries Karan Fries 4.24-5.99 2.46-3.98 4.34-5.76 2.76-3.80 1700 1700 4.86 3.20 4.84 3.20 522 522 Karan Fries 7.99-9.16 3.00-5.68 Karan Fries 3907 (157) 4497 (149) 1700 1700 2.73 (159) 41.43 (52) 8.71 4.27 337 (149) 3603 (50) 4168 (48) 425 (91) 165 (91) 415 (28) 153 (33) 363 (47) 75 (91) 67 (33) Buffaloes Murrah Buffaloes Murrah 5.04-6.83 3.07-4.68 5.05-6.22 3.19-4.45 Buffaloes 9.13-10.92 3.55-10.44 Murrah 1270 1270 5.81 3.87 5.81 3.91 308 308 41.87 (36) 2431 (82) 2333 (82) 2007 (26) 2209 (26) 2.24 (82) 1270 1270 Murrah 439 (31) 129 (64) 9.79 7.90 101 (31) 343 (82) 130 (20) 342 (26) 470 (8) 94 (8) Artificial BreedingResearchCentre Figures inparenthesesindicatenumberofanimals • • • Research Highlights and buffaloes. dissemination of superior germplasm of cattle management andfrozensementechnology training facilityonanimalbreeding/reproduction advance germplasm, semenprocessinglaboratory, bulls, cryopreservationfacilityforstorageof dairy animals/ male of management scientific The centeralsostrengthenedthefacilitiesfor of germplasmtofarmers. Parlour fordistribution buffalo bull, storage for dry fodder and Semen developed infrastructure formaintainingcloned Breeding BullCenterinthecountry.Thecentre -02 andMurrah-44),isthelargestInstitute Tharparkar-13, Karan-Fries- 34, Karan Swiss with acapacityof131bulls(Sahiwal-38, (ABRC) Centre Research Breeding Artificial The Lactation length(days) Lactation number Animal number Milk yield(kg) Lactation number Animal number Milk yield(kg) Best Lactation305days

ABRC. at available are % 60 than more PTM with strengthened and 6500 doses of frozen semen bull (Shresth)intheworldwasfurther buffalo cloned first of freezing semen The reduced to16.7and18.0months. of the young Murrah bulls has significantly initiating training and age at first ejaculation ejaculation inMurrahbulls.Averageagefor semen first at age and training initiating for management practicestoreducetheage feeding developed has centre The produce goodqualitysemen. not donated semen consistently) started to sahiwal bulls(more than threeyearsagebut eight of out five result a as and mucous) application of bio-stimulation (estrous innovative the developed centre The 1783 4686 1783 4063 427 5 5 • • • • • • 1274 2719 1274 2655 311

1 1 cattle. for multiplicationofTharparkar breedof seven out of16Tharparkarbullsmaintained bulls. Thecenteralsopreservedthesemenof crossbred bulls underProgenyTesting ofKF Fries Karan fifteen and Cattle’ Sahiwal of bulls under AICRP on ‘Genetic improvement on BuffaloImprovement”,sixteenSahiwal nine Murrahbullsunder“NetworkProject collected andpreservedthefrozensemenof of breedingbulls:During2013-14,thecenter Management and cryopreservation of semen production ofgermplasm. develop measures for improving furtherthe to bulls ofbreeding study the behaviour recording electronic the initiated centre The per MSPofcryopreservationsemen. found within the range of microbial load as semen of 59 breeding bulls and all bulls were of load microbiological screened centre The abnormalities. bulls werefound freefrom anychromosomal abnormalities of78breedingbullsandall chromosomal the screened centre The transmitted diseases. from sexually samples werefoundnegative semen ofbreedingbulls.Allbullsand Trichomoniasis of all breeding bulls and andBrucellosis, IBR,Campilobacteriasis andviraldiseasesviz.,TB, JD,bacterial transmitted sexually screened centre The information ofbulls. and softwareformonitoringmanagement The centre procured some critical equipments 4410 6107 4402 5830 379 1 3 11301 6791 6791 8511 479 5 5 5240 3915 562 498 498 2 2 157 ndri annual report 2013-14 158 ndri annual report 2013-14 Himachal Pradesh,Karnataka, MadhyaPradesh. Uttarakhand, U.P, Rajasthan, Bihar, Chhattisgarh, gaushalas of 11states viz., Delhi,Haryana,Punjab, dairy developmentorganizations/institutes/ KF andMurrahbullstofarmers andvarious 1,04,949 doses frozen semen of Sahiwal, Tharparkar, different states and also disseminated/supplied of Sahiwal, KF and Murrah bulls to farmers of disseminated 23,430mldosesliquidsemen of cattle and buffaloes. During theyearABRC improvement programme germplasm forgenetic The centreisdisseminatingsuperiormale Dissemination ofSuperiorGermplasm 1, 80,997dosesoffrozensemen and buffaloes. During2013-14,thecentre produced conservation ofsuperiormalegermplasmcattle The centre is involved inproduction and Production ofSuperiorGermplasm efficiency ofNDRIherds. reproductive the monitored and bulls breeding of insemination artificial 1293 performed center The Reproduction Management –– ArtificialBreeding Research Centre –– Over AllConceptionRate Particulars Conception Rate3 Conception Rate1 Conception Rate(%) No. ofService/Conception Av. AgeatConception(Month) Av. AgeatMaturity(Month) No. ofObservations Heifer No. ofService/Conception Service Period(days) No. ofObservations Cow/Buffalo 14 6136 6066 6044 6014 th 4 - - - - Set Murrah Bulls rd st service. service 15 6410 6290 6139 6007 6333 th 5 Reproductive StatusofNDRIHerdduring2013 - - - Set Breeding bullsatABRCduring2013-14 10 1817 1815 1727 1681 1852 1854 1909 1958 th 115.54 8 77.82 42.90 48.36 29.18 28.04 Set 1.47 1.32 SW 44 82 Sahiwal Bulls Govt. Frozen SemenStation,BAIF, JindandTripura mandatory trainingofARS, and participantsfrom training tonewlyappointed ARS scientists under Registration Scheme,Rohtak. ABRCimparted Dharoli, DistrictJindandstockman ofCentralHerd programme forworkersofFrozenSemenStation During theperiod, ABRC conducted four training Training Organized activities. to thefarmers inuse ofA.I., different breeding and Internationalparticipants.Advisoryservice farming forDAHDF,MOA,Govt.ofIndia,PLDB frozen sementechnologyandcommercialdairy breeding, artificial of aspects various on modules agencies/farmers. ABRCdevelopedTraining - ThreeandMurrahFive)togovernment (Karan Fries-Five,SahiwalFour,Tharparkar The centre distributed17surplusbreedingbulls 50.00 85.71 50.00 29.40 29.40 98.82 11 1.20 1.41 TP 2037 2030 2019 2004 2056 2066 2073 2094 29 5 th 8 Set 120.71 Breed 32.55 62.50 50.00 26.00 23.00 2.00 1.71 KS 1 7 12 7141 7134 7096 7088 7178 7205 7249 th 7 - Set Karan FriesBulls 116.96 35.40 67.75 40.65 28.19 27.04 1.38 1.33 113 KF 21 13 7322 7304 7289 7242 7462 7485 7521 7517 th 8 141.13 Set 42.58 76.04 44.27 32.51 29.42 MU 2.00 1.32 33 91 the graincomponentoffeed. programmes of institute and partial fulfillment of out therequirementsfortransferoftechnology of fodder seed andothergraincrops tomeet of fodder, some area is utilized for production the daytorequirement herd. Aftermeeting out nutritionalrequirementsoftheInstitute quantity ofgoodqualitygreenfoddertomeet and ManagementCentreistoproduceadequate Research ofForage The foremostresponsibility Centre Forage ResearchAndManagement semen during2013-14. the institutethroughsaleofliquidandfrozen ABRC generated`13,68,530/-for of theinstitute, and buffalogermplasmtoincreasetherevenue Looking in tothedemand and quality of the cattle Revenue Generation Crop Total Jower +Bajra Cowpea Bajra Makchari Maize +Makchari Cowpea +Bajra Maize +Jowar Jowar (Multicut) Jowar (Singlecut) Maize Mustard Lucerne Oats +Maize+Mustard Turnip Oats Berseem +Mustard Wheat Barley Crops Total Oats Production andProductivityofForageCropsinformGreenFodder November 2013 May 2013 Month Total Straw ProductionofWheat,Barley,OatsandPaddy Production andProductivityofGrainCrops Area(ha) 434.19 113.28 Area (ha) 19.37 23.82 40.00 13.86 97.52 13.44 13.24 66.56 2.30 8.38 8.90 3.24 2.75 5.91 1.62 72.11 50.41 17.65 4.05 Wheat Straw Paddy Straw Barley Straw 204451.25 qof fodder including 194741.50qgreen of grown. Duringtheperiodunderreportatotal were also grain cropsofoats,wheatandbarley turnip andwintermaizeinrabiseason.Similarly and lucerne,berseem,oatschinesecabbage, grass hybridsandcowpeaduringkharifseason fodder cropsofmaize,sorghum,sorghumSudan was producedfromhighyieldingvarietiesof In all,1,83,265.75quintalgoodqualitygreenfodder Oats Straw Total Transfer to PDC Meerut (excluding Pondarea) Hostels, PowerHouse,PlayGround Dairy MelaGround Dairy DemonstrationUnit(KVK) Farm Seed ProductionSection/Forageresearch Forage ProductionSection Unit Allocations oftheFarmLandtodifferentUnits Average yield(q/h) Crop Average yield(q/h) 519.23 154.86 288.28 466.91 562.96 131.09 172.73 600.96 260.44 254.85 478.68 396.10 437.03 639.06 348.57 823.35 34.51 39.98 9.27 - - Production (q) Production (q) Qty (q.) 1,379.75 1024.25 193.75 83.00 78.75 1,83,265.75 24038.75 24853.00 39485.50 54802.50 1,277.45 1194,25 1299.75 5584.00 4155.50 1824.00 4114.50 3609.75 6433.50 2341.00 8461.25 467.40 139.75 670.30 360.50 708.00 Area (ha) 357.22 2.83 3.44 13.52 40.25 256.68 40.5 159 ndri annual report 2013-14 160 ndri annual report 2013-14 Forage Production Section. Presently 16 varieties of Forage Production Section. Presently16varieties of the yearaforage museum was established at To maintain thegreenfodder availability round Forage Museum:2acre(87120squarefeet) made bushsystemwasalsointroduced. Teflon use. efficient for done were modification cum-seed spreaderwerepurchasedandsome implements likeRotoseeddrillandfertilizer- work efficiency,qualityofsowing.New new implementsandequipmentsforincreasing The ForageProductionSectionatNDRIintroduced Mechanization atNDRIFarm Total RevenuegenerateRs.28,65,901.25 Rs. 28,65,901.25Revenuewasgenerated. produced underRevolvingFundSchemeandtotal 17198 q. green fodder and 307.50 q. dry fodder was During theyear48.97q.seeds,191.50grains, Production Revolving FundSchemeonSeedGrain saved bytheuseoffarmproduceasseed/feed. realized andanamountofRs.18,38,580.00was tractors on lease anamount ofRs. 18,78,914.36 Through sale of farm produce and providing Revenue Generation silage andwheat/oatsstrawweresupplied. DM contentinthelushgreenforagesisverylow, required drymatterduringwintermonths,when provide herdduringleanperiodand to of the requirements nutritional the fulfill To produced. straw intheformofby-productthesecropswas of dryfodderaswheat,barley,paddyandoats conserved assilage11020.50qand1,379.75 was oats of fodder green of quantity Sufficient Forage Conservation of wheat,barleyandoatswas1277.45q. supplied tocattleyard.Totalproduction of grains fodder 8816.00 qsilage and 893.75 qdry fodder was Berseem Crop was was milk products. 2013-14 is`4,81,75,427/-fromthesaleofmilkand year financial the during generated revenue The HACCP-15000 byBIS,Chandigarh. management systemISO9001-2008and Experimental DairyiscertifiedunderQuality of ExperimentalDairy. being utilizedfordevelopment of infrastructure 1989-90. Revenuegeneratedthroughschemeis running under Revolving Fund Scheme since Experimental Dairy-selfsustainingdairyhasbeen were providedtrainingduringtheyear2013-2014. A totalno.of81studentsfromseveralinstitutions of various universities/colleges in the Dairy field. It also provides training facility to outside students scientists oftheNDRIDeemedUniversity. training facility to students and research facility to Experimental Dairyprovidespracticalteachingand Special Features Institute’s mainentrance. are soldthroughtheMilkParlourlocatedat cream, processedcheeseetc.Theseproducts ice lassi, kalakand, burfi, paneer, ghee, butter, dairy products such as,milkpowder,pasteurized used for convertingthesurplus milk into varietyof requirements of research and teaching, the plant is the operationofdairyplants.Aftermeeting to provide training facilities to thestudents in in the laboratories on the pilot scale as well as scaling upofnewproducts/processes developed infrastructure facilitiestothescientistsfor institute withthe objective of providing necessary Experimental DairyPlanthas been setupatthis Experimental DairyPlant systems together with multi-function laser printers. systems togetherwithmulti-function laserprinters. equipped withstate-of-the-art 64 bit i5 computer Computer Laboratoryfor students’teaching, to post-graduate students. There is awellestablished courses to under-graduate studentsand one course The ComputerCentreofferstwoScience administration; andtrainingtostudents/scholars. computational supporttothescientistsand Computer Centre is acentral facility to provide Computer Centre turnip wascultivatedatforagemuseumsite. berseem, twovarietiesofoatsandonevariety at foragemuseum. In rabiseason three varietiesof 1Anjan-3, paragrass-1, Bahia grass-1) wereplanted perennial grasses (napier-7,Guinea-3,Nandigrass- to research projects of the Institute including Institute ofthe researchprojects to pertaining data scientific processed Centre The Scientific DataProcessing support forthisERPsystem. Centre extendeditstechnicalandICTinfrastructure Computer The office. paperless and transparent implemented theMIS/FMSERPsolutionfora In anewinitiativebyICAR,theInstitutehas carried outbytheCentre. as well generation ofvarious reports wasalso bills andGPFtransactionspertainingtothestaff house developedsoftware.Theprocessingofpay support to theappropriate authorities using in- animal managementsystem, etc.,fordecision supply offeedsandfodders, herd performance, monthly MIS reports relating to milkproduction, Computer Centreprepareddaily,fortnightlyand Management InformationService exam atthiscentre. ICAR. Inall570candidatesundertooktheonline NET/ARS-Preliminary ExaminationbyASRB, Examination byASRB,ICAR”forconducting an onlinesystemforNET/ARS-Preliminary Commissioning, OperatingandManaging “Developing, NAIPSub-project, under the An onlineexaminationcentrehasbeenestablished ASRB OnlineExaminationCentre languages compilers. FORTRAN, C, C++ and Visual Programming round theclock.Thecomputersystemssupport students toaccesstheInternetandlibraryresources Institute LANthroughWi-Finetworktoenablethe and students.Studenthostelsarealsoconnectedto all the Scientists, Technical/Administrative Officers located buildings,therebyprovidingconnectivityto buildings andthroughADSLswitchesfordistantly through optical fiber/UTP cabling to cover the main Network (LAN) connecting all the divisions/sections The Institute has a well established Local Area NET 2014(I)OnlineExaminationconducted bytheASRBinprogressatNDRIKarnalCentre date. regularly updaedtokeepheinformationup-to- security featuresinplace.Thewebcontentwas rolebased isdynamicwith The NDRIWebsite Website level andnationalgovernmentagencies. university relatedinformationtovariousstate under ComputerCentre,whichdisseminates (HYPM). Also Statistical Cell is functioning and HalfYearlyProgress Monitoring System Agriculture EducationNetwork(NISAGENet), (PERMISNet), NationalInformationSystemfor Personnel ManagementInformationSystem implementation ofICARprogrammelike filtering, etc.AKMUisalsoundertakingthe prevention, applicationcontrolandWebcontent management), including firewall, VPN, intrusion (antivirus/antimalware, antispam, vulnerability devices enforce essential security mechanism and Network-Analyser-200DforAKMU.These procured state-of-the-artUTMFortiGate-600C Management (UTM)System.ComputerCentre by ERNETIndiaLtd.,viaUnifiedThreat India andthroughleasedline(1Mbps)provided National InformaticsCentre(NIC)Govt.of the provided by Network node(1Gbps) connectivity throughNationalKnowledge fully functionalwiththeInternetand e-mail (AKMU) establishedwiththehelpofICARis The AgricultureKnowledgeManagementUnit Agriculture KnowledgeManagementUnit computing software. developed scientific programme and SAS statistical scientists and162researchscholarsusingin-house analysis consultancyto190users comprising 28 data scientific provided Centre Computer The SSCNARS) isextensivelyutilisedforthepurpose. under theaegisofNAIPfundedsubproject: statistical computingsoftware(recentlyacquired by theresearchscholars.ThesophisticatedSAS students dissertations/thesesworksubmitted –– ComputerCentre –– 161 ndri annual report 2013-14 162 ndri annual report 2013-14 Exhibitions Organised Revenue Generation libraries withintegration/collaborationofOnline to have a union catalogue of all participating (eGranth)”. The project has major mandate and Information Management underNARS Sub-project “StrengtheningofDigitalLibrary The Library, NDRI is an active partner of NAIP Issue-Return andreservationfacilities. Document Scanning,PrintingandComputerised CD-ROM. TheLibraryalsoprovidesPhotocopying, on products milkanddairy including products Indian StandardsandISOonfood Abstract, DerwentBiotechnology AGRIS, Abstract, ROM ofCABAbstract,FoodScienceTechnology Services, CD-ROMLiteraturescanningthrough Documentation, Reference,CurrentAwareness The LibraryprovidesInternet,Email, advanced researchareasandforcommunication. Students use these togetcurrent information inthe workstations forstudentsandstaffoftheinstitute. fifty having section computer excellent an has Library CDs. 1,500 microfiches, 268 theses, 4,186 includes 51,460books,32,457boundjournals, developments. There are89,871volumes which technical scientific/ current ofthe track keep More than 200 periodicals are subscribed to of literature onDairyScience and related subjects. has animpressivecollection Library The Institute Library 9 7 5 4 2 1 2. 1 3 12 11 10 6 13 8 Krishi VasantatC.I.C.R.Nagpur. Buffalo MelaatCIBR,Hisar. Trade FairatPragatiMaidan,Delhi. Innovation-cum-Seed DayatDWR,Karnal. National WorkshoponOutScalingFarmInnovationatNASC,Delhi. Indian DairySummit-2013atDelhi. Kisan MelaonMaizeandSugarCaneatUchani,Karnal. Rabi KisanMelaatCSSRI,Karnal. National DairyMelaatNDRI,Karnal. Progressive PunjabAgricultureSummitatChatiChiri(Mohali),Punjab. Kisan SammellanatJajjhar,(Haryana). Kisan Mela organized byYoungAssociation atVillageRakharaDist. Patiala(Punjab) Kisan MelaatSugarcaneBreedingInstitute,Karnal. Total Photography Revenue fromDr.D.Sundaresan Auditorium VCD weresupplied. etc. werecarriedoutinICARand92Nos.ofDVD/ programmes of theInstituteandCattleshows, Dairy Mela,Exhibitions,KisanSangosthi,Cultural Convocation, Meeting, Conferences, National consisting of National & International Seminars, Video coverage of 55events of the Institute Scholars for theirresearches & research projects. &research staff,students were providedtothe & VCD, dubbing & mixing and photos editing The facilities of audio video recording on DVD Audio-Video andPhotoLab. Communication Centre Management System. on URL:library.ndri.res.in by usingKoha-Library complete onlinelibrarycatalogueisalsoavailable and researcharticlesetc.Inadditionofabove publications, reports,conferenceproceedings outputs, whichincludesvaluable books, institute Library digitized ~500 contents/records of records ofLibrary,NDRIavailableonWorldCat. in MARC21format.Presently~49,250catalogue journals and other resources on OCLC plateform catalogue/ databaseofalltheavailablebooks the participating libraries are to uploaded their Computer LibraryCenter(OCLC)”.Inthisproject 9 1 14 15 3 24 16 1 25 16 19 15 5 st th th st rd March,2014 February2014 th th th th th th th th -13 February,2014 -5 March,2014 -27 -19 January,2014 -27 October,2013 September,2013 September,2013 ` 9,25,085.00 ` 10,154.00 ` 9,14,931.00 th th September,2013 th th th February,2014 February2014 February,2014 November,2013 miscellaneous worksoftheInstitute. maintenance ofSTPandBioGasPlantsother and inspection of new buildings in theInstitute; sewage disposal systemintheInstitute;planning Authorities; maintenanceofthewatersupplyand Pollution ControlBoardandLocal State Haryana power failure; Liaison works with HSEB, CPWD, providing generator supply to office area in case of area as well as residential area of the Institute; maintenance ofelectricsupplytotheoffice overhead lines, street lights,service connections the labs/Institute;maintenanceofsub-station, etc.; newworksaddition/alterationsrequiredin electrical, civil,refrigerationandairconditioning providing theservices related to mechanical, Since 1979,Maintenance Sectionhasbeen Maintenance Section one formineral mixtures and oneforbakery for milkprocessingofdifferent milkproducts, with TBI under incubation programme, two organised. Fourincubatee companiesworked Animals for“OrissaGovernment” werealso Reproduction HealthManagement inDairy and onetrainingprogrammeonBreeding on Infertility Management for veterinary officials Farming and two Short Training Programmes Dairy Commercial on five and products milk Three coursesinDairyProcessingandrelated organised duringthefinancialyear2013-14. & EntrepreneurshipinDairying”(SINED) society inthenameof“SocietyforInnovation asa supportof DSTandregistered the with Technology Business Incubator (TBI) established Technology BusinessIncubator biochemical analyzer. automatic haematolyzerandasemi-auto a fully etc. Diagnostic clinical lab is well equippedwith various types ofmuscular, arthritis and ailments, back-ache, cardiac ailments, hypertension and from variouschronicailmentssuchaslower suffering patients the of benefit the for premises facilities. A Physiotherapy Unit exits at the complex assist theDoctorsinprovidingdesiredmedical staff, laboratorytechniciansandpharmacists nursing trained and qualified Well medicine. of Allopathy, Ayurveda and Homoeopathy systems in aid medical render Officers Medical Karnal. at well astotheothersisterICARInstitutessituated needs of theemployeesandstudentsatNDRIas established in1991.Itiscateringtothehealth The Human Health Complex (HHC) was Human HealthComplex • Special Features System (FSMS)ISO22000:2005. certified undertheFoodSafetyManagement in fullcapacity.ModelDairyPlantispresently (Ltrs.) ofmilkperdayandispresentlyrunning The Planthasbeendesignedtohandle60,000liters basis bytheNationalDairyDevelopmentBoard. turnkey on installed and assistance financial the established during1995atNDRI,Karnalthrough A state-of-the-art Commercial Dairy Plant was Model DairyPlant • • • • graduated in2013-14. and twoothervirtualincubateecompanies products. One virtual incubatee company joined

N.D.R.I. Deemed University duringthe4 training to thestudents of B.Tech. (DT) of the Model Dairy Plant provides one year In-plant is presentlyprocessing/packing 70–80 toned, doubletonedandskim milk).MDP standard, cream, (full variants different five and packaging of milk for Mother Dairyin processing in engaged currently is MDP of NDRI. a committee, whose Chairman is theDirector unit of ICAR, is independently managed by autonomous an (MDP), Plant Dairy Model scale undercommercialenvironment. concepts fromlaboratoryscaletoindustrial the scientistsofNDRIforscalingupR& D to facilities infrastructure provides also It problems inproductionmanagement. instills confidenceinhandlingreallife the moderncommercialDairyPlantand in gaining sufficient experience in managing infrastructure fortraining,whichhelpsthem complete with provided are students The no. ofstudentshavebeentrainedsofar. year ofthecoursecurriculum.Atotal451 th

163 ndri annual report 2013-14 164 ndri annual report 2013-14 • –– ModelDairyPlant –– •

Federations and its production is not taken butter purchasedfromdifferentState cow from ghee cow manufactures MDP Dairy. etc. andaspertherequirementsofMother Shahi Malai, Kewra, Thandai, Butter Scotch, different varietiesi.e.Vanilla,Strawberry, The icecreammanufacturedatMDPisof utilizing thefullcapacityofunit. thereby average for240daysoperation) day (yearly Mother Dairytothetuneof7000litersper MDP also processes and packs Ice-cream for for MotherDairy,Delhi. TLPD of polypack Milkinallthevarieties Dr. G.R.Patil,JointDirector(Academics) receiving'EducationLeadershipAward-2013' • •

packed atMDP. demand of different milk variants being day dayto meet to which issufficient per dayisaround60,000to70,000LPD, NMG rates. The average milk procurement and theirNGCmilkcollectioncentreson Dairy fromtheunitsofPunjab/Rajasthan Mother of behalf on milk receives MDP the MDPSaleCounter. manufactured atMDPisbeingsoldthrough sale of ghee 20-25MT/month. All the ghee as per demand.The average production/ cheese, paneerontrialbasis. manufacturing ofcottagecheese,processed in students to training impacts MDP

16 facilities areprovidedtoall thesectionsthrough the computers of different sections and internet A LAN withamodern web server networks students. communication and programming packages for to facilitatedataanalysis,documentation,e-mail There arenetworkconnected computer facilities various dairyproducts,effluentanalysis,etc. testing ofdairyequipments,manufacturing and microbiological analyses of dairy products, molecular genetics, screening of microbes, chemical facilities forcarryingoutresearchworkon The Campushasgoodlaboratoryandinfrastructural herd oftheInstitute. breed and50crossbred cattle constitutethedairy herd oftheInstitute.About 250zebucattle ofDeoni for meetingthefeedingrequirementofdairy utilized forthecultivationofvariousforagecrops area of46.3hectares.About21hectareslandis hostel facilities, etc.The campus envelopes a land staff dispensary, dairy herd,laboratories,library, infrastructure in terms of qualified staff, farmland, The campus is endowed with necessary Infrastructure • • • Mandate mandate andobjectives. region. The Station functions with thefollowing dairy farmersandindustryofthesouthern the research,trainingandextensionneedsof Station ofNDRI.Thestationhasbeencateringto Bangalore continuedastheSouthernRegional Quarters toKarnalin 1955, the establishmentat dairy industry. Upon shifting of the institute Head to meetthemanpowerrequirementsofNation’s institution instarting dairy education programmes Husbandry andDairying.Itwastheforerunner at Bangalorein1923asImperialInstituteofAnimal The NationalDairyResearchInstitutewasstarted

REGIONAL STATIONS Campus ofNDRIDeemedUniversity. D and HRD with the stationas the Southern Establish centers of advanced studies for R & assessment anddissemination. Establish centers for technology development, solutions. on continuing basisandevolvesuitable production, processingandmanagement Identify the region specific problems of dairy S outhern R egional S to theentrepreneurs.Theexcess ofmilkisbeing from variousinstitutions.Training isalsoimparted also providingin-planttraining tothestudents programmes andis involved inregulartraining is Thesection products. andmilk various milk renovated andhasallthefacilities formanufacturing The experimental dairy of the Instituteis recently Technical staff. staff strength of 164 including 22 Scientific and 38 and EducationTraining.TheCampushas Dairy Economics and Statistics,DairyExtension Chemistry andBacteriology,DairyEngineering, Forage Production, Dairy Technology, Dairy and ArtificialInsemination,CattleYard through differentsections viz., Animal Breeding programmes attheCampus arecarriedout The research,trainingandtransferoftechnology developed recently. auditorium withverygoodfacilitieshavebeen conferences, etc.,aseminarhallandmini seminars, ofmeetings, For facilitatingconduct up preliminaryinvestigations. facilities fortaking of thedispensaryalsohas The clinicallaboratory needs of students, staff and retired employees. dispensary attheCampuscaterstomedical andvisitors.The staff ofstudents stay for the There isagoodhostelandguesthousefacility has alsofacilityforonlinejournalreferral. and food science across the country.The Library students and professionals in the field of dairying The Libraryservesasareferralcentreformany Services and Inter Library Loan facilities to readers. Services, Reference News PaperClipping facility, also providesinternetservices,photocopying have beenupdatedintothesoftware.TheLibrary thebook about details and 9859Bibliographical for facilitatingtheautomationoflibraryfunctions abroad. Library Automation Software procured Institutions/organizations ofthecountry and also receivedongratisbasisfromdifferent Science. Inaddition,fewjournals were of Dairy Foreign Journalsrelevanttovariousdisciplines 1,295 reprints.Librarysubscribesto125Indian/ bound volumes of journals, 2,018 theses and The libraryisstockedwith12,467books.10,857 also beenestablished. laboratory forevaluationofdairyproductshas functioning atthecampus.Asensoryevaluation effluent-testing laboratory are established and are the server. Aneffluenttreatment plantand tation , B angalore 165 ndri annual report 2013-14 166 ndri annual report 2013-14 Training Programmes(2013) • • A totalrevenueof Animal Nutrition, Dairy Economics and Dairy Genetics and Breeding, Animal Biotechnology, Dairy Chemistry, DairyEngineering, Animal students inthedisciplinesofDairyTechnology, Engineering, guidance is being provided to the PG Tech Programme in DairyTechnologyand course workforPh.D.inDairyEngineeringandM. Dairy Extension.Inadditiontoconductingthe Animal Genetics & Breeding, Dairy Economics and Engineering, Chemistry, Dairy Technology, Dairy work arebeingofferedinthedisciplines of Dairy Ph.D. Programmesforcarryingoutdissertation Educational Programmes Education andTraining through theparlourofInstitute. also convertedintodifferentdairyproductsforsale of milkis sold asmarketmilkandsomequantity –– Southern Regional Station,Bangalore –– Scholarship/Fellowship Total Project Work Preparation ofCheeseandotherRelatedProducts Orientation onSensoryEvaluationofCheese,DahiandYoghurt In-lab Training(DT) In-lab Training(DE) In-plant Training(DT) Training onLivestockGenomicsandIPProtectioninBiotechnology Propagation andMaintenanceofStarterCulture Dairy CattleFeedAnalysisandformulation Commercial DairyProduction Name oftheTrainingProgrammes INSPIRE (SRF) RGNF-JRF ICAR-SRF/JRF NDRI Fellowship

Celebrations. SportswerealsoconductedCelebrations. the HindiDayandNational Festival for thestudentsofourcampus during Literary and cultural activities are conducted and AcademicInstitutions. MehsanaMilkUnion Agro ProductPvt.Ltd., India Pvt.Ltd.,GCMMF,IDMC,Hatsun the variousorganizationslikeM/s. Wrigley and recruitmentprocesswasconductedby the PGstudents for theircareer advancement to imparted was counseling and Guidance ` 2,46,846/-wascollectedtowardsthetrainingfeesforaboveprogrammesduringyear2013. • • of NDRI,Bangaloreon1 in DairyTechnologyProgrammewas held at SRS Counseling processforadmissionDiploma tothe programme. 2013. Thirtystudentshavebeenadmitted to this Technology has been started from August, A new programme viz. Diploma in Dairy the laboratoriesofStation. Universities also conducted their project work in other Colleges/Universities. PG students of other training wasgiventotheUGandPGstudentsof Apart fromthis,in-planttrainingandin-lab operative Federationsand Private Organisations. farmers/entrepreneurs and personnel from Co- Production andExtensionforthestudents,dairy Assurance, Dairy imparted inProcessing,Quality work. ShorttermTrainingProgrammesarebeing outtheirdissertationExtension forcarrying

Ph.D. Training inDecember2013. (Maharashtra) completed theirIn-plant Campus, MAFSU,Latur, DistUdgir College ofDairyTechnology, Veterinary from students year 3rd (DT) B.Tech Eight training inJune,2013 Latur (Maharashtra)completedtheirin-plant College ofDairyTechnology,Pusadand from students year final (DT) B.Tech Nine Day Celebrations. during theIndependenceDayandRepublic 02 01 02 10 No. ofBatches 17 03 01 01 01 01 05 01 01 01 03 st August,2013. No. ofParticipants M.Tech. 02 23 -- -- 87 03 05 15 02 01 22 03 04 04 30 • • • • Extension Activities •

Veterinary University,Tirupathion27 Technology College, Sri Venkateshwara organized for thirteenstudents from Dairy was programmes orientation day One Thrissur, KeraladuringJune2013. four batchesofProgressiveFarmersfrom Orientation programmes were organized for to startupacommercialdairyfarm. cultures for food industry and on knowhow and milk products, availability of dairy products, packaging and marketing of milk for dairybusiness,exportpotential and farmers,preparationofprojectreports programmes indairyingforruralwomen indigenous dairyproducts,training aspects, know–howon preparationof technologies andscientific dairyfarming operated milk machines, dairy processing informationregarding handdairy breeds, ofhighyielding availability project, dairy feasibility ofmodern and mechanized mega included needs information of profile The mail correspondenceandphoneenquiries. personal visitstotheInstitutethrough thirty oftheneedyclienteleduringtheir the to rendered was advice Technical activities. needs and wereexplained abouttheongoing the Institutetovariousunits/labsaspertheir the Institute.Thevisitors were takenround farmers, entrepreneurs andtraineesvisited educational institutesofsouthernregion, comprising ofstudents from various batches 22 in visitors 558 of no. total A at the institute was organized on 4 programme training cum visit exposure An 2013. Department ofAgriculture,Govt.Kerala. Thrissur, KeralaunderATMA programme, 2013 fortheIIHRtraineefarmersfrom Farmers' trainingprogramme th April th July • • • • • • •

31 on organized was programme refresher A (MANAGE), Hyderabad, Agricultural Extension Management by SRS,NDRIandNationalInstituteof Functionaries in Dairy Sector” was organized forExtension “New DimensionsinExtension on Programme Training Collaborative A Govt. fromdifferentstates. andState fromvariousCentral and Fisheries Department ofAnimalHusbandry,Dairying the Animal Husbandry Officers / Directors of Trainers’ Trainingprogrammefacilitatedfor ICAR andUAS,Bangalorefrom23 India KVKMeet2013jointlyorganizedby All on Conference National in Participated Centre (BIEC),Bangalore. 2013 atBangaloreInternationalExhibition women friendly technologieswas exhibited women empowermentshowcasing of husbandry and dairying. As aneventfor animal horticulture, agriculture, of field the on various income-generative activities in woman beneficiaries into creating awareness Bangalore. Theinitiativewasencouragethe 19 technologies heldduring23 & Processing,PackagingandAllied Products on Dairy Exhibition International 2013, India Tech Dairy in Participated Nadu, PondicherryandKarnataka. Tamil Meghalaya, Kerala, states ofPunjab, Animal Husbandry Departments from the Directors /AssistantfromState fourteen participants comprising Deputy The training programwas well attended by Sciences (UAS), from 7 2013 organizedbyUniversityofAgricultural Mela Krishi International in Participated of AgriculturalSciences(UAS),Bangalore. October, 2013atGKVKCampusofUniversity on 10 Kasargod, KeralaunderATMA programme request fortheprogressivedairyfarmersof Dairy FarmingPractices” was organized on One day training programme on, “Scientific during 7 organized byIIHR,Hesaraghatta,Bangalore Mahotsav (Women inAgriculture Day) Mahila Krishi Rashtriya in Participated Bangalore. CampusofUAS GKVK 2013 heldat th st May, 2013 for the NIANP trainees on September 2013 at SRS, NDRI Bangalore. September 2013atSRS,NDRIBangalore. th April2013. th and8 th December, 2013 at Lal Bagh December,2013atLalBagh –– Southern Regional Station,Bangalore –– th to11

held from16 rd to25 th November, th August, rd to25 th to to th

167 ndri annual report 2013-14 168 ndri annual report 2013-14 Milk andProductsSale(January2013toDecember2013) Experimental Dairy –– Southern Regional Station,Bangalore –– • • Dr. S. Ayyappan, Hon'ble Secretary, DARE and DG, ICAR at the Dr. S.Ayyappan,Hon'bleSecretary,DAREandDG,ICARatthe 1. S. No. 2. 3. 4. 5. 7. 8. 9. 10. 11. 12. 13. 14.

Krishi MahilaMahotsav2013organisedbyIIHRBangalore 14 atcptd n 42 in Participated NDRI participatedintheevent. of villages adopted the from beneficiaries was created during this event. Women for theirfarmandhome-madeproducts opportunity andmarketing participated Karnataka, AndhraPradeshandTamilNadu entrepreneurs fromvariousdistrictsof by theparticipatinginstitutes.Farmwomen Association (South Zone)heldduring12 Conference (DIC)organizedbyIndianDairy entrepreneurs and dairy farmers of the locale. dairy of benefit the for Tamil in prepared folder on‘CleanMilkProduction’was needed technicalinformation.Anextension visitors andneedyclientsfordisseminationof processing aspects was distributed to the Extension literature on dairy production and Center Chennai. th December, 2013 held at ChennaiTrade Milk sweet,kg Product Flavoured milk,pkt Process cheese,pkt Paneer, kg Butter, kg Gulabjamun mix,pkt Cheese purimix,pkt Chhana podo,pkt Kunda, cups Ice cream(S),cups Ice cream(O),cups Ice cream(O),FP Ice cream(S),FP nd DairyIndustry th to • • • • New Initiatives • •

Quantity sold development on12 on dynamics and group dairy entrepreneurship Ittamadu andP.Rampuravillagesweretrained Bidathi from members group SHG Two rural development. and dairying of field the in working NGOs involves association ofNDRIwithinterested Women: An Action Research. This project Entrepreneurship Development ofRural through implementation of IRC project SHGs women rural of Empowerment systems. constraint analysis of thesmall dairy farming status intheregionwhichincludes to elucidate thepresent dairy farming trend/ states southern ofallthe systems production dairy holder small on initiated was study A Dairy EducationatFarmersDoorProgram. implement Rural Extension Programme and to identified was villages of cluster new A milk andproducts. organized forthepubliconimportanceof June, 2013and awareness campaign was NDRI by students and staff of NDRI on 1 of SRS at observed was Day Milk World Rajankunte on8 SHGs ofselectedvillages,Suradenapuraand on scientific dairying was organized for women An interactive meeting and awareness program 1435.5 397.5 270.5 5702 5312 1091 1862 332 356 670 473 49 87 th July 2013. July2013. th July2013. Amount (`) 398400 238900 95400 58412 75740 14940 16020 56300 14000 18620 5676 4410 7370 Dairy Dairy st

be 808.03±87.0kginalactationlengthof201.31 purpose cows during lastoneyearwasfoundto The meanlactationmilkyieldofDeoni,thedual Deoni animalswasrecorded as 1460.0±67.9days. of calving first at age mean The respectively. kg, animals were3026.86± 149.50and 4992.63±379.33 monthly milkyieldforDeoniand HF crossbred indigenous breed.Duringlastoneyear,themean maintains “Deoni”,animportantdualpurpose Livestock farmlocatedatNDRI,SRS,Bangalore Livestock Farm a vitallinkbetweentheNDRI, Karnalandthefar- extension activitiestothese problems. Itservesas India and to offersolutions through research and of dairy productionineasternand north eastern Regional Stationistoidentify themajorconstraints The mainobjectiveofestablishing theEastern The maininstituteislocatedatKarnal,Haryana. premier institutedevoted to researchondairying. The NationalDairyResearchInstitute(NDRI)isa functioning withinthesamecampusfromMay,1987. laboratory buildingandtheentirestationstarted were graduallybuiltup.TheStationitsown where cattlesheds, forageunit,staffquarters,etc. West Bengalgranted100acresoflandatKalyani effect fromJune1,1968.In1978theGovernmentof Bengal, wasmergedwiththeE.R.S.ofN.D.R.I. the I.C.A.R.tillthenlocatedatHaringhata,West The RegionalAnimalNutritionResearchCentreof the AdministrativeBuildingofKalyaniUniversity. about 50kmnorthofCalcuttaandwaslocatedin shifted toKalyaniduring1966,Nadiadistrict, the CentralDairyinCalcutta1964andwas The EasternRegionalStationwasestablishedat HF Crossbred 15. Deoni Breed 16 17. 18. 19. 20. Total Yoghurt, cups Curd, pkt Khoa, kg Cream, kg Skim milk,lit Milk, lit Productive andReproductivePerformance(Mean±SE)ofDairyHerd Lactation (in days) 201.31± length 13.9 305 E Lactation 808.03± (in kg) 3013 ± yield 190.8 astern 87.0 Daily milkyield per lactation(in R 10.0 ±0.6 3.7±0.2 egional kg) S Section, GuestHouse,etc. Cattle Herd,FodderFarm, Library,Computer infrastructure facilitieslikeResearch Laboratories, started functioningduring 2005. TheStationhas and DairyExtension.Animal BiotechnologySection Economics&Statistics Forage Production,Dairy Production and Management, Animal Breeding, is mainlyfocusedonAnimalNutrition,Livestock Economics andDairyExtension.Since1986research Nutrition, Animal Breeding, Soil Science Dairy and duringtheperiodof1977-1985,relatedtoAnimal Nutrition and Dairy Chemistry and Bacteriology, the periodof1972-1976,itwas relatedtoAnimal During to AnimalNutrition. related were mainly The researchworkduringtheperiodof1964-1972, farmers ofthisregion. improve thesocio-economicconditionofdairy applied innatureandthethrustofresearchisto undertaken atthisstationis mainly strategicand Theresearchwork forperfection. trial after at theinstituteand provides appropriate feedback of the country for transfer of technology developed flung areas of the eastern and north eastern regions 398.71 ±23.7days. mean calving intervalinHFcrossbred cows were ± 0.62kgand21.63.19days,respectively.The and days toattainpeakyieldwere10.0± 0.6; 14.8 The meanvaluesfordailymilkyield,peakyield day lactationyieldwas recorded as3013±190.8kg. 446.83 ±23.7days.InHFcrossbredanimals,305 The meanintercalvingperiodinDeonicows was 3.7±0.2; 5.7±0.5kgand10±1.4daysrespectively. and days to attainpeakyield werereported as ±13.9 days.Themeandailymilkyield,peakyield attain peak tation Days to 10±1.4 yield 21.6± 3.19 45814 2568 979 140 10 26 , K 5.7±0.5 (in kg) yield 14.8± Peak 0.62 alyani (48.7 months) 1460.0 ±67.9 Age atfirst (in days) calving - 1266772 2322967 10231 30816 2400 5200 3360 (13.3 months) Inter calving 398.71 ±23.7 446.83 ±23.7 (15 months) (in days) period 169 ndri annual report 2013-14 170 ndri annual report 2013-14 to-day basis. and meteorologyarealsobeingmaintainedonday- fodder andfeedsupply,reproductiveperformance references. Inaddition,thedataofmilkproduction, VSAT, whichisusefulforsearchingliteratureand The institutehasinternetconnectivitythrough huge database and analysis of experimental data. for reference.Thecomputercenterfacilitatesthe various workshopsandseminarsarealsoavailable Reports of different Institutes and proceedings of periodicals in the field of Dairying. Besides, Annual books, 3360volumesofboundjournalsandother to theinstitutefarmanimals.Thelibrarycontains1505 offoddercrops areaforsupplying crops in27hectare section isengagedincultivationofqualityfodder cattle belongingtoJerseycrossbreds.FodderFarm The CattleYardofthestationmaintainsaround150 extension work. are activelyengagedinresearch,trainingand Dairy ExtensionandBiotechnologywhich AnimalBreeding,Dairy Economics, Management, Animal Nutrition,LivestockProduction& section etc.Therearesixresearchsectionsnamely Laboratories, Library,GuestHouse,Computer Farm, Research Cattle Herd,FodderProduction Research Institute hasinfrastructurefacilitieslike Dairy ofNational station regional The Eastern Support Sections in thisregion. great role to play in the field of dairy development economic conditions;thisresearchstationhasa resources and diversified agro-climatic and socio- of thenativestock,shortagefeedandfodder the easternregion,lowmilkproductionpotential Keeping inviewtheenormousdemandformilk –– EasternRegional Station,Kalyani –– Overall Average December November October September August July June May April March February January Months (2013-2014) Milk ProductionPerformanceatERS-NDRIherd(JanuarytoDecember,2013) Milk Production 110999.0 10011.0 10192.0 10720.0 8534.0 7708.5 8298.5 9631.0 9821.0 8935.5 9660.0 8777.5 8709.5 (kg) Wet Average (kg) 6.34 6.06 5.74 5.73 6.20 6.59 6.91 7.16 6.32 6.39 6.80 6.52 5.63 Livestock Farm farming activity. suitable technologies inorder to enhance dairy being madebytheInstitutetopopularizelatest village extensionprogrammes whereallefforts are for transfer through these trainings, field visits and up taken time. Thefeasibletechnologiesarebeing for dairyfarmersarealsobeingorganisedtimeto Management” and “AI and Veterinary FirstAid” on “ScientificDairyFarming”,“DairyCattle Trainingprogrammesand NGOsonrequest. for the farmers of Dairy Cooperative Societies programmes are organised at regular intervals visits, Farmer-ScientistInterface,orientation research work ofM.Sc. and Ph.D.scholars. Field This station has laboratory facilities forthe Reproductive PerformanceofERS-NDRIHerd Mortality (%) Inter calvingperiod(days) Service period(days) Conception rate(%) No. ofpregnantcows No. ofinseminatedcows Age atfirstcalving(month) Herd average(kg)/day Wet average(kg)/day Av. no.ofcow’sdry/day Av. no.ofcow’sinmilk/day Total milkproduction(kg) Herd strengthason31.12.2013 Particulars Herd Average (kg) 4.49 4.12 3.97 4.20 4.52 4.63 4.70 5.08 4.56 4.45 4.67 4.68 4.25 Average FAT 4.69 4.78 4.78 4.78 4.75 4.76 4.76 4.69 4.73 4.82 4.75 4.75 5.48 % Average SNF% Jersey Cross 110999.0 8.66 8.64 8.64 8.64 8.63 8.64 8.63 8.63 8.62 8.61 8.57 8.57 8.59 58.2 38.8 4.49 6.34 19.7 48.0 432 114 153 9.8 46 79 compost and alsofortrainingdemonstration compost unitusedforproductionofvermin- tractors, chaff cutter, etc.There is a small vermin- servicing ofagriculturalmachineries including forregular alsohasaminiworkshop Section Besides cultivationoffoddercrops,theForage either chaffedorunchaffedtotheCattleYard. manages harvestingandsupplyoffoddercrops quality foddercrops in about27hectaresareaand Forage FarmSectionisengagedincultivationof Forage Farm • Part ofIndia inNorthEastern HillRegion/EasternFarming of TribalPeopleThroughIntegratedLivestock TSP-Upliftment of Socio-economic Condition literature andreferences. through VSAT,whichisusefulforsearching of database. The Institute has Internet connectivity maintenance facilitates the The ComputerCenter Computer Centre Presently 20journalsaresubscribed. and seminarsarealsoavailableforreference. institutes andproceedingsofvariousworkshops of Dairying.Besides,AnnualReportsdifferent field the in periodicals other and journals bound The Librarycontains1505books,3360volumes of Library Total Hybrid napier+guineagrasspara Sorghum /sorghum+cowpea,sorghum+ricebean Oats/oats +mustard Maize /maize+cowpea Berseem/berseem +mustard,lucerne/lucerne+mustard,cowpeasole Type offodder

during theprogrammeatBali Island.Atotal were rendered healthcheck upand treatment 555 cows, 483 goat560poultryand47sheep farmers who rear livestock. A total number of fodder grasswerealsodistributed tothe (maize andcowpea)rooted slipsofsome Somefodderseeds Bengal ofWest district organized intheAyodhyahillsPurulia Health andvaccination camps werealso were demonstratedtothelivestockfarmers. dairy production management systems South 24ParganasofWestBengal.Different Bali Islandof camps wereorganizedinthe vaccination two and camps health Two Production ofDifferentFodderCropsatERSFarmduring2013-14 • crop production. theoretical and practical part of training infodder Section hasnecessaryfacilityforcoveringthe and demonstrationpurpose.TheForage training premises. Besides, thereisafodderherbariumfor of teak,shesham, direction, rainfall,etc.Thereareabout700plants temperature atdifferentdepth,windspeedand humidity, max.andmin.airtemperature,soil for variousmeteorologicalparameterslikerelative observations aretaken observatory whereregular purpose. Thereisanagri-meteorological • • •

the DepartmentofAnimal Resource Livestock Development Officers from 2014 to 31 NDRI fortribalwomenfarmersfrom12 Milk Production”wasorganizedatERS, “Clean on programme training day 3 One at AyodhyaPahar,Purulia. goats werevaccinatedduringtheprogramme programme atBaliIslandand152cows,206 cows, 195 goatswasvaccinated during the of250Pahar, Purulia.Atotalnumber treatment during the programme at Ayodhya 21 sheepwererenderedhealthcheckupand number of 459 cows, 352 goat, 73 poultry and March to14 ERS, NDRIfortribalfarmersfrom24 Dairy FarmingPractices”wasorganizedat One 8 day training programme on “Scientific participated. tribe womenfarmersofNadiadistrict from 5 Region for Tribal Farmers” was organized Dairy FarmingPractices for North Eastern “Scientific on programme training day 9 A actively participated. of Jalpaiguri district of North Bengalwere organized on20 Production forLivestock Feeding”was “Azolla on Demonstration and Training A Territorial Council(BTC),Assam. Farmers (under TSP Project) for Bodoland th –13 st March 2014. Twelve tribal farmers March 2014. Twelvetribal farmers etc. growing around the Institute etc. growing around theInstitute th th March2014.TwentySanthal December,2013for15Tribal th December,2013.Eighteen –– EasternRegional Station,Kalyani –– Production (Quintals) 12512.75 4789.60 2971.00 2272.30 1850.95 628.90 th March March th

171 ndri annual report 2013-14 172 ndri annual report 2013-14 • • Extension Activities • • • –– EasternRegional Station,Kalyani –– • •

Deworming” campwasorganizedon21 and “Anoestrous programme day One animals fortreatment. three (33) farmers came with eighty three (83) Chandamari” villageofNadiaDistrict.Thirty “Veterinary andFirstaidCamp” wasalso pukur, North24Parganas.One programme were adoptedofficiallyin2 Chandamari ofNadiadistrictWestBengal Dakhhin and Muratipur villages Two was organized on28 camp “Deworming” programme day One thirty (30)animalsfortreatment. Nadia District.Eleven(11)farmerscamewith February 2014intheMuratipur villageof organized on17 frontline fodder demonstration ” was camp, “vaccination programme day One Nadia district. dairy animalstonearerclusterofvillages providing A.I.facilitiesandtreatmentof for wasestablished village in Muratipur by thisinstitute.One“DairyVikashKendra” 22 on Mela Krishi in participated NDRI ERS, twenty five(25)animalsfor treatment. District. Eighteen(18)farmerscamewith the DakhhinChandamari villageof Nadia December, 2013. Development Officers(BLDO)on31 training totwentytwoBlockLivestock Nimpith, WestBengal,ERS-NDRIimparted invitation fromRamkrishnaAshramKVK, azolla cultivationwasorganizedonspecial on demonstration and training Campus Off attended theTrainingProgram. Development, Nadiadistrict,WestBengal was organizedon24 camp” “vaccination programme day One animals fortreatment. farmers camewithonehundredten(110) village ofNadiaDistrict.Fiftyone(51) nd February2014atChaltaberia Dutta th Jan2014intheMuratipur th Jan2014intheDakhin th February2014in nd February 2014 February st st

• • • • • • • •

methods. practical demonstrationofAzolla Cultivation Fourteen farmersparticipated instepby Dakhhin Chandamari villageon20 were organizedfor30farmwomenin demonstrations azolla cum training Two Kalna II,Burdwanparticipated. WBCADC, of official and members Group February 2014.ThirtynumberofSelfHelp - 29 One day exposure visit was organized on 17 actively participated. from MuratipurvillageofNadiadistrict Fourteen number ofwomen dairy farmers of Burdwan district on 30 organized atBurarvillage,RainaBlock was camp veterinary-cum-infertility A mela organizingcommitteeforthefarmers. programme wasalsoorganizedwithkrishi with farmers.OneDairyquizcompetition provided treatmentto20animalsandinteracted organized bythisinstitute.TeamofERS Producers UnionLtd.Krishnanagarfrom3 collaboration with Kishan Co-operative Milk dairyfarming practices”was organized in on “Dairy cooperatives and advance scientific Two days Orientation /Induction programme anoestrus andotherhealthrelatedproblems. farmers. Inall132animalsweretreated mixtures @500gmweredistributedto34 132 animals attended this camp. Mineral 2013. Atotalof52dairyfarmerswith in Muratipur village on14 in Muratipur organized at premises of one farmer’s house was demonstration azolla Frontline One Block of Nadia district on 20 (adopted villageofERSNDRI),Chakdah organized atDakhhinChandamarivillage was camp health veterinary-cum-animal A related problems. treated foranoestrousandotherhealth and Jagulipara).Thirtyfivecowswere adopted villagesofKVK,Budbud(Atpara 2014 and1 of CRIJAFon13 Budbud andKVK, of NDRI;ERSIVRI Farmers’ InterfacewasorganizedwithERS programme HealthCamp-cum-Scientist collaboration Inter-institutional One programme with50animals, Forty dairyfarmerswerepresentonthat Field day was organized on 20 on organized was day Field different districtsofWestBengal. th March2014for135dairyfarmersfrom st February2014. th August,2013inthetwo th February 2014. February th th July 2013. November th Jan2014. th January January rd th

ds fu;a=.k esa gSA laLFkku esa bl le; bl esa laLFkku gSA esa fu;a=.k ds ,oa dqylfpo ds fu;a=.k eas gSA tcfd foÙk&foHkkx dEiVªksyj LFkkiuk rFkk lqj{kk ds dk;Zdyki la;qDr funs'kd (iz'kklu) lqfoèkk,a miyCèk gSaA iz'kklfud dk;Zdyki tSls% Ø;] HkaMkj] laink vuqHkkx rFkk vuqj{k.k bathfu;jh vuqHkkx tSlh dsUnzh; dsUnz] daI;wVj dsUnz] tSolwpuk jk"Vªh; ,oa lsok iqLrdky; ,dd] ijke'kZ la;a=] Msjh iz;ksxkRed bUD;wcsVj] fctusl vuqlaèkku ,oa izcUèku dsUnz] i'kq LokLF; ifjlj] VsDuksyksth pkjk QkeZ] i'kqèku esa laLFkku gSaA Hkh dsUnz vuqlaèkku iztuu i'kq ,oa dsUnz izf'k{k.k Msjh rFkk dsUnz foKku Ïf"k (,fVd) dsUnz lwpuk izkS|ksfxdh Ïf"k ,d esa laLFkku gSaA gksrs laiUu vUrxZr ds izHkkx izcUèk ,oa lkaf[;dh vFkZ'kkL= Msjh rFkk foLrkj Msjh lw{ethofoKku] Msjh jlk;u] Msjh bathfu;fjax] 'kjhjfØ;k foKku] i'kq tho jlk;u] i'kq tSo izkS|ksfxdh] Msjh mRiknu ,oa izcUèku] Msjh i'kqiks"k.k] pkjk vuqlaèkku] Msjh i'kq i'kqèku iztuu] i'kq Msjh izHkkxksa@vuqHkkxksa rsjg ds laLFkku dk;ZØe laLFkku ds eq[;ky; rFkk blds nks {ks=h; dsUnzksa ij lg;ksx ds fy, iw.kZ :i esa ls fodkl lefiZr lalkèku jgk ekuo gSA rFkk o"kZ fodkl ,oa vuqlaèkku fy, ds dk;ZØeksa fodkl Msjh esa ns'k fd tks gS laLFkku vuqlaèkku jk"Vªh; Msjh vuqlaèkku laLFkku jk"Vª dk ,d vxz.kh ,oa izfrf"Br fodkl ds rhu eq[; {ks= vkSj vuqlaèkku ds laLFkku gSaA djrs iznku lg;ksx esa djkus funs'kd dks vuqlaèkku f'k{k.k ,oa foLrkj dk;Zdykiksa dks laiUu bl laLFkku dk dk;Zikyd vfèkdkjh gSA nksuksa la;qDr funs'kd] ifj"kn ,oa foLrkj ifj"kn lfefr;ksa dks lkSaik x;k gSA funs'kd fo|k ifj"kn] lykgdkj vuqlaèkku eaMy] izcaèk nkf;Ro dk fu.kZ; vkSj uhfr&fuèkkZj.k esa {ks= ds dk;Zdyki iz'kklfud vkSj f'k{kk foLrkj izf'k{k.k] f'k{k.k] vuqlaèkku] ds laLFkku gSA gh vuq:i ds i¼fr iz'kklfud dh fo'ofo|ky; ekU; laLFkku dh izcUèk iz.kkyh Hkkjrh; Ïf"k vuqlaèkku ifj"kn ds laxBukRed Lo:i ntkZ izkIr gSA dk;ZØeksa ds lapkyu gsrq laLFkku dks ekU; fo'ofo|ky; dks fy, vuqlaèkku ,oa lg;ksx iznku djus esa yxs gq, gSaA 'kSf{kd dsUnz LFkkuh; {ks= esa Ïf"k okrkoj.k ds vuq:i Msjh fodkl ds tks fd caxykSj vkSj dY;k.kh esa fLFkr gSA nf{k.k o iwohZ {ks=h; LFkkukUrfjr dj fn;k x;kA bl laLFkku ds nks {ks=h; dsUnz gsSa esa LFkkfir bl laLFkku ds eq[;ky; dks o"kZZ rFkk (iii) Msjh foLrkj@izcUèku gSaA lHkh vuqlaèkku ,oa foLrkj (i) Msjh mRiknu oSKkfud] 157 1955 esa djuky esa (ii) 1923 esa caxykSj Msjh izlaLdj.k fgUnh lkjka'k 260 rduhf'k;u] mYys[kuh; gS fd laLFkku ls ckg~; foÙkh; lgk;rk izkIr djus esa lQy jgk gSA ;gka ;g lh-,e-vkj-) rFkk i'kqikyu ,oa Ms;fjax foHkkx (Mh-,-,p-Mh-) (vkbZ- ifj"kn vuqlaèkku fpfdRlk Hkkjrh; (Mh-,-bZ-)] foHkkx ÅtkZ ijek.kq (,e-,Q-ih-vkbZ-)] ea=ky; m|ksx izlaLdj.k vuqlaèkku ds fy, jk"Vªh; QaM (,u-,Q-ch-,l-vkj-,-)] [kk| lw{etho C;wjks (,u-ch-,-vkbZ-,u-) Ïf"k ij ewy ,oa egRoiw.kZ jk"Vªh; Msjh fodkl cksMZ (,u-Mh-Mh-ch-) jk"Vªh; Ïf"k mi;ksxh (Mh-,l-Vh-) foHkkx izkS|ksfxdh ,oa foKku (Mh-ch-Vh-) foHkkx yxHkx lHkh vxz.kh jk"Vªh; QafMax ,tsfUl;ksa tSls tSoizkS|ksfxd vFkok lgk;d lg;ksxh gksxkA jk"Vªh; Msjh vuqlaèkku laLFkku gSA ftuesajk"Vªh;MsjhvuqlaèkkulaLFkku;krksusr`Rodjsxk vuqlaèkku ifj;kstukvksa dks foÙkh; lgk;rk nsuk Lohdkj fd;k dqN ls :i la;qDr us cSad fo'o gSaA lfEefyr ifj;ktuk,a vuqlaèkku la;qDr U;wthySaM baMks rFkk vk;jySaM baMks ,-Mh)] jgh gS ftlesa teZu 'kS{kf.kd fofue; lsok,a (Mh-,l-Vh&Mh-,- bl le; rhu vUrjkZ"Vªh; vuqlaèkku leUo; ifj;kstuk,a py esa vc rd lcls vfèkd gSA jk"Vªh; Msjh vuqlaèkku laLFkku esa igqap xbZ gS tks fd jk"Vªh; Msjh vuqlaèkku laLFkku ds bfrgkl QafMax ls ifj;kstukvksa vuqlaèkku foÙkh; vfrfjDr fy, ds djus lqn`<+ dks dk;ks± vuqlaèkku vuqlaèkku ifj;kstukvksa dk dk;Z py jgk FkkA ewy ,oa egRoiw.kZ o"kZZ vuqlaèkku Hkh lfEefyr gSA ctV dk dsUnzksa {ks=h; rFkk dsUnz foKku Ïf"k fo'ofo|ky; ctV dqy fy, ds 'kh"kZ ;kstuk xSj ,oa ;kstuk fy, ds 2013&14 ctV O;; okLrfod esa o"kZZ dk laLFkku ctV dk;Z djus ds fy, izksRlkfgr fd;k gSA lwpukvksa ds ifj;kstuk ij vkgkjksa fØ;kRed rFkk U;wVªkL;wfVdyl nkc] ,ck;fVd esa i'kqvksa {ks=h; uSuksizkS|ksfxdh] ck;kslSalj] ,Ecz;kstsfufll] VªkalfØiVksu] Dyksfuax] dh HkSal dksf'kdk] LVse dks oSKkfudksa us lgk;rk izkIr ls ,tsfUl;ksa QafMax vU; ,oa ,u-,-vkbZ-ih- ifj;kstuk dk lapkyu dj jgk gSA lsokjr gSA ds nkSjku dqy nkSjku ds 2013 14531-09 yk[k :i, LohÏr fd;k x;kA blesa ekuuh; 145 iz'kklfud ,oa esa ;kstuk ,oa xSj&;kstuk 'kh"kZ xSj&;kstuk ,oa ;kstuk esa 2013&14 yk[k :i, Fkk rFkk o"kZZ rFkk Fkk :i, yk[k 14501-47 ` vUr%laLFkkuh; rFkk vUr%laLFkkuh; 89

1-85 djksM+ jkf'k dh ,d ijke'kZ 550 fuiq.k lgk;d deZpkjh djksM+ :i, rd :i, djksM+ 132-35 ckã 75 173 ndri annual report 2013-14 174 ndri annual report 2013-14 ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ vuqlaèkku dh gSA iznku lgk;rk Hkh fy, ds p;u ds rduhfd;ksa ,oa midj.k vkèkqfud esa fucaèkksa 'kksèk vius dks Nk=ksa us vuqnku izkIr ls ,u-,-vkbZ-ih- x,A fd, izkIr QaM Hkh fy, ds djus lqn`<+ dks vadhdj.k rFkk ifjdyu ,oa ewY;kadu rFkk ekuhVfjax –– ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ fgUnh lkjka'k dh vksj fofHkUu ln`';rk iznf'kZr djrs ik, x,A vkbZ-th-vkbZ rFkk ,u-Mh-,-5 vfHkxzkgd Mh-,l-vkj-,u-, vU; Lruèkkfj;ksa esa ik, tkus okys thu ds leku gSaA vkj- Mksesu laxBu ds iSVuZ dk vuqlaj.k djrs ik, x, tks fd HkSal ds fØ;kRed vkj-vkbZ-th-vkbZ- rFkk ,e-Mh-,-5 thu zS+ dh s kckCkV kfkkka s izo`Rr ls dksf'kdkvksa cgqiztuu{ke LVSe QkbcjksCykLV dksf'kdk,a mRiUu dh xbZA ds cdjh izkS<+ dh s Ezkfd Ve kfkkka s fMEck.kqtu dksf'kdk,a ls mRiUu dh xbZA dksf'kdkvksa LVSe ,Ecz;ksfud ds cdjh fd, x,A fy, HkSal ds Lruh; ,ihFkhfyy dksf'kdkvksa esa vfHkO;aftr Ekuo bUlqfyu thu Vªkalthfud dksf'kdkvksa esa mRiknu ds fodflr fd, x,A fy, ds VªkalQSD'ku esa dksf'kdkvksa LVSe ,Ecz;ksfud dh HkSal fy, ds vfr&vfHkO;atuk mudh dUlVªDV thu 2 ,Q-th-,Q- rFkk ,y-vkbZ-,Q ,u-,-,u-vks-th-] ds HkSal fd, x,A mRiUu CykLVksflLV DyksuM iztkrh; vUr% djds iz;ksx ?kjsyw HkSal (cqcsyl cqcsfyl) ls fMEck.kqtudksf'kdkvksa dk oU; HkSal vjuk (cqcsyl vjuh) ls iznkrk dksf'kdkvksa rFkk jgkA mRiUu djus esa fd;k x;kA ;g cNM+k dks cNM+s bl iz;ksx dk dksf'kdkvksa lkseSfVd fo;ksftr ls o"kZZ iwoZ gks pqdh FkhA bl lkaM ds iz'khfrr fge nzfor oh;Z Fkk pwafd ;g ,d ,sls lkaM dk Dyksu Fkk ftldh e`R;q dbZ fnukad ua0 lkaM ijhf{kr larfr ,d jghA ,d Js"B lkaM ,e-;w- lkaM Js"B ,d flrEcj] tUe dk ^iwf.kZek* dVM+h DyksuM ,d ls gS mRiknd nqXèk mPpre esa bfrgkl ds laLFkku fd tks dhfrZ* ^d.kZ dksf'kdkvksa ds }kjk mRiUu gksus dh fof'k"Vrk izkIr gSa LVSe fo;ksftr ls oh;Z rkts bles rFkk Fkk fdyksxzke 55 Hkkj bldk le; ds tUe rFkk gqvk mRiUu }kjk izlo fnukad 10 vxLr] –– dks mRiUu gqvkA ;g dVM+k lkekU; dVM+k ;g gqvkA mRiUu dks 18-32010 dks gqvkA ;g dVM+h ;g gqvkA dks 2013 2013 dks mRiUu gqvkA ;g dVM+k Js"B dk DyksuM dVM+k ^Lo.kZ* dVM+k DyksuM dk 5926 ls ,d DyksuU dVM+k DyksuU ,d ls 4393 fnu rd thfor rd fnu 21 12 ?kaVs rd thfor 6

¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ Ýht xk;ksa (29-56 xk;ksa Ýht fu;ksftr eSFkqu ds fy, Js"B xk;ksa ds :i esa dqy leku ikbZ xbZA ckabfMax MkbukfeDl nwèk ls fo;ksftr ns'kh ysDVksQsfju ds ykSg dh jsd&,y-,Q- x,A fd, mRiUu esa [kehj ,Q-) (jsd&,y- ysDVksQsfjul dh cdjh rFkk HkSal iqul±;kstd mRiUu dh esa xbZA dksfy rFkk (,Q-ch-ih-bZ-) izksVhu (lh-ch-ih-) ckabfMax QkbczksuksfDVu izksVhu ckabfMax dkysxu iqulaZ;ksu ij bldh ckè;rk fuèkkZfjr dh xbZA lh-,-3) izksVhu mRiUu fd;k x;k rFkk fMEck.kqtudksf'kdkvksa lEcfUèkr ,Økslkse LieZ iqul±;ksth dk HkSal x;k rFkk ,d Lruh; dksf'kdk ykbu ls ifj'kq¼ fd;k x;kA fd;k mRiUu rRo fujksèkd Y;wdsfe;k iqul±;ksth dk HkSal kaka h Hkèk l lcfk ikVus ds vkblksQkeZ vuqØfer fd, izksVhuksa x,A lacafèkr ls xHkkZèkku dh HkSalksa fØ;kRed foHksnh dj.k esa Hkwfedk gks ldrh gSA xbZ ftudh fd HkSalksa dh Lruh; ,fiFkhfyy dksf'kdkvksa ds fofHkUu izdkj ls vfHkO;aftr lksyg izksVhuksa dh igpku dh xbZA dh igpku dh ck;ksekdZjl laHkkfor rhu ds nqXèkL=o.k dh igpku dh xbZA ldsarksa izksVhu esa dksf'kdkvksa ,fiFkhfyvy Lruh; nkSjku nqXèkL=o.k iznf'kZr laHkkO; ekxZ dh fofHkUu voLFkkvksa ds nqXèk&mRiknu rFkk nqXèk L=o.k vofLFkfr ds lkFk lEcUèk esa loafèkZr fd;k x;kA rFkk bUVjY;wfdu vfHkO;atuk dks ekius ds fy, ij[kuyh ,u-,y-vkj fcuk rFkk lkFk ds fo|ekurk dh vkj-vkj- ih- fof'k"V fofHkUu dks dksf'kdkvksa ukfHkdh; eksuksa jDr ckã ls HkSalksa rFkk xksi'kqvksa Hkkjrh; x;kA fd;k vè;;u dk vfHkO;atuk izkjafHkd dh thuksa ,u-,y-vkj- fofHkUu esa VkfUly) rFkk Lihyhu (jDr] vo;oksa bE;wu fofHkUu ls vfHkO;aftr ik, x,A dk;Z dk è;ku fd, fcuk] fofHkUu Årdksa esa loZO;kid :i ds Ård esa :i ds vo;oksa bE;wu vkj-,y-thu ds HkSal 13-90 vis{kk dh fd-xzk-) bZ-ih-,-(4114 vkSlr rFkk fd-xzk-) vkSlr bZ-ih-,- mRiknu nqXèk vkSlr dk fnu 305 vkSlr nqXèk mRiknu rFkk vkSlr % rFkk 5528 fd 4620 fd 12-29 % ) dk p;u fd;k x;kA budk vkSlr budk x;kA fd;k p;u dk ) 4158 rFkk -xzk FkkA % vfèkd FkkA Js"B FkkA vfèkd -xzk Fkk tks fd i'kq vkSlr (4250 8648 fd rFkk fd-xzk 4841 -xzk ds chp Fkk fnu dk fnu 305 (,l-ih-,- 3 fofHkUu 11 68 dju

¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ fnu vFkok de nqXèk&L=o.k dky nqXèk mRiknu nqXèk dky nqXèk&L=o.k de vFkok fnu 305 Js"B MSe rFkk (bZ-ih-Mh-) vUrj iwokZuqekfur iwoZ visf{kr lØhfuax LFkkukUrj.k laokgd ik, x,A lkbVkstsusfVdy dh cNM+ksa ikap tUesa ;s izR;kjksi.k Hkwz.k HkSalksa esa FkuSyk dh de laHkkouk FkhA eqjkZg thuksVkbiM ,-ch- lkFk ds 1881&vkj-,Q-,y-ih- vU; thuksVkbi dh rqyuk esa lh-Mh-14 thu ds ,p-ih-okbZ FkhA laHkkouk vfèkd esa i'kqvksa ,-,-lh-lh-,p-,p-thuksVkbi rFkk Fkh laHkkouk de dh jksx FkuSyk esa HkSalksa eqjkZg okyh ysDVksQsfju thu ds ,-,-lh lh th-th- la;qdr thuksVkbi egRoiw.kZ lEcUèk ugha ik;k x;kA 100 , ;g Øe'k% 0-0102 rFkk Øe'k% ckjEckjrk lEiw.kZ dh ,-,-thuksVkbi rFkk xksi'kqvksa esa lkghoky xbZA dh fodflr vkj&vkj-,Q-,y-ih-,LlSa ,l dj iz;ksx dk lh-,p-oh ,p-ih-okbZ- fy, ds thuksVkbfiax ,l-,u-ih- esa xksi'kqvksa 2-036 ds ch rFkk 8 100 , (dsyxzsuqfyu) thu dss lkghoky rFkk dju Ýht xksi'kqvksa esa lEiw.kZ dksfM±x rFkk Mh- 4348&6861 fd-xzk- ds chp FkkA p;fur cNM+ksa dk bZ-ih- dju Ýht cNM+ksa dk Mse Js"B vkjf{kr x;kA j[kk vkjf{kr dks cNM+ksa Ýht dju 18 dqy ij vkèkkj ds bZ-ih-Mh- rFkk vuq:irk 'kkjhfjd ,oa Js"B ds Hkfo"; esa iztuu ds fy, ;qok cNM+ksa ds p;u ds fy, MSe esa dju Ýht xk;ksa esa fnlEcj gksekstkbxksVl ds :i esa thuksVkbiMM FksA xksi'kq (111) lkghoky lHkh tcfd Fkh Øe'k% ckjEckjrk dh thuksVkbi th esa xksi'kqvksa (114) Ýht rFkk ds-ch- ftad fQaxj (,Q-bZ-tsM-,y-) tSls thulo Øe'k% ,Ecz;ksfud QksLczsu esa xksi'kqvksa Ýht d.kZ rFkk Lkkghoky dks iz'khfrr oh;Z dh dqy {ks= esa vkuqoakf'kdh lqèkkj ds fy, fofHkUu ,tsfUl;ksaa@Ï"kdksa dh xbZ rFkk Ï"kdksa ds i'kq lewgksa esa 545 cNfM+;ka) mRiUu gqbZA 3562 Ïf=e xHkkZèkku esa ls 5-03 rFkk 8 thuksVkbi rFkk uSnkfud FkuSyk dh ?kVuk esa dksbZ fnu ds nqXèk mRiknu] iztuu fo'ks"krkvksa iztuu mRiknu] nqXèk ds fnu 305 0-973] 2 (,-@th-) ,l-,u-ih-yksdj ij th-th-]th-,- ds-ch- rd foLrkfjr ik, x,A d.kZ x,A ik, foLrkfjr rd ds-ch- 3-789 0-013 ikbZ xbZ tcfd d.kZ Ýht xksi'kqvksa esa 12-28 2-080 ds-ch-fo|eku FksA 0-02 rFkk % ds chp FkkA 215 Mkst csph xbZA th rFkk th th@13 13 2012 rd {ks=h; ifjfLFkfr;ksa 0-007 FkhA xksi'kqvksa esa ,l 47-04 , 100 305 fnu ds nqXèk mRiknu 5* Qysafdax {ks=ksa esa Øe'k% 1167 ( % rFkk 0-965 xHkkZèkku nj izkIr ds fy, ih-lh- fy, ds 8 th th@13 13 622 cNM+s rFkk th@13 12 0-884] 3-772 0-035 ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ fu"iknu {kerk ds fy, Vh-,p-vkbZ Js"B dh fo'ks"kdksa tuu{kerk esa HkSalksa eqjkZg vUrxZr ds ,p-vkbZ- Vh- FkzslgksyM+ fy, ds fo'ks"kdksa tuu{kerk esa HkSalksa eqjkZg vkiwfrZ dhA dh oh;Z iz'khfrr dks laxBuksa fodkl Msjh rFkk Ï"kdksa us dsUnz laLFkku vuqlaèkku Msjh jk"Vªh; dh vis{kk 3151-29 fd-xzk- rd c<+ x;k tks fd i'kq mRiknu lewg ds vkSlr nqXèk L=o.k nqXèk vkSlr dk HkSlksa eqjkZg Js"B ds fy, ikap eqjkZg >ksVksa dks p;fur fd;k x;kA HkSal lqèkkj ij usVodZ ifj;kstuk ds vUrxZr ijh{k.k eSFkqu eqjkZg dVM+ksa dks Hkfo"; esa iztuu gsrq vkjf{kr j[kk x;kA (2747&4090 fd-xzk- ds chp) ds vkèkkj ij dqy nl Js"B zsh min yxHkx mRiknu izksVhu la'kksfèkr fd, x,A isybV vkgkj dh rqyuk esa lw{etSoh; dksf'kdh; fHkÙkh dh ikP; iks"kd vUr%xzZg.k rFkk lsY;wyksl ij lEiw.kZ lkUnz.k fMtkbu ij vkèkkj ds xfr fd.ou ewY;kadu fd;k x;kA ij dsUnzksa oh;Z O;kolkf;d ukS fLFkr esa Hkkxksa fofHkUu ds Hkkjr nf{k.k vkfFkZd gkfu mBkuh iM+rh gSA miuSnkfud FkuSyk (,l-lh- Hkkjh dks Ï"kdksa Msjh ftlls gS gksrh o`f¼ blls esa ykxr mipkj rFkk gS djrk deh esa mRikndrk ;g rks gS gksrk miuSnkfud FkuSyk] FkuSyk dh uSnkfud ;fn gSa :i dkj.k esa fof'k"V LFkkukUrfjr rFkk izeq[k ,d dk {kfr vkfFkZd mPp dks Ï"kdksa Msjh (,l-lh-,e-) FkuSyk miuSnkfud fe-xzk-@,e-,y-ds chp) ikbZ xbZA ds nwèk esa ysDVksQsfju dh ek=k mPprj (48-98 ls thoudky esa iwjs vius us xk;ksa esa la[;k dkQh FkkA ekl 14-91$093 esa lqèkkj ik;k x;kA fMtkbuM lkUnz.k lEiwjdksa (lh-,l) ds lkFk ,l-,u-,Q- yhVj] 40 Js"B mRiknu rFkk vUr%&C;kWar vofèk Øe'k% esa vkSlr nqXèkL=o.k nqXèk mRiknu] nSfud nqXèk mRiknu] ls yksdfiz; gSA {ks=h; ifjfLFkfr;ksa ds vUrxZr Js"B xk;ksa uke ds o"kkZxaèkh ;g rFkk gS nsrh nwèk rd ekl ukSa rFkk gS Hkwfedk fuHkkrh gSaA xk;sa izfro"kZZ ,d cNM+s dks tUe nsus okyh ,oa Hkkjh o"kkZ okys eyukn dh {ks=h; iz.kkyh esa egRoiw.kZ {ks=ksa rVh; ds dukZVd rFkk gS Qyrh&Qwyrh ij pjkbZ vfèkdrj fd tks gS xksi'kq cksSuh Js"B ,d fxnk eyukn ls 17 % ) la'kksèku gqvkA fd.ou xfrth ds vkèkkj ij vkèkkj ds xfrth fd.ou gqvkA la'kksèku ) 75 vkadh xbZA miks".kh; thyok;qoh; ifjfLFkfr;ksa 47-60 vfèkd FkkA yhVj] 2-17$029 15 cNM+s mRiUu fd,A eyukn fxnk xksi'kqvksa 'kSMksa esa j[ks x, j[ks esa 'kSMksa 21 15 % li.Z kl o`f¼ vkSlr (lEiw.kZ hj rFkk yhVj 3-42$039 iztud lkaMksa dk lkaMksa iztud 627 <

75 gksuh pkfg,A Mkst+ dh Mkst+ 62]054 –– 522-33$69 fgUnh lkjka'k 157-10 13 –– 175 ndri annual report 2013-14 176 ndri annual report 2013-14 ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ –– ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ fgUnh lkjka'k miyCèk gksrk gS] dh vuqdwyre mRikndrk cUkk, j[kus ds ckjs esa ;g lkspk tkrk gS fd ;g gjs pkjs ls i;kZIr ek=k esa mRikndrk ij izfrdwy izHkko iM+rk gSA foVkfeu , mudh ftlds dk rkacsa tcfd gS gksrk lqèkkj esa mRikndrk ,oa vlaØkE;rk mudh rks gS tkrk fd;k lEiwfjr fooksa esa foVkfeu bZ- rFkk ,- ,oa ftad tc Msjh i'kqvksa ds ifjikjV; nkSjku vfHkO;atuk dk egRo Hkh vfèkd FkkA ds ½rq xzh"e ij vofèk;ksa lHkh esa rqyuk dh ifjfLFkfr;ksa i'pkr izlo rFkk izloiwoZ nkSjku ds ½rq xzh"e rFkk ifjfLFkfr;ksa ,u-,- vfHkO;aatuk C;kWus ds ,e-vkj- dklist+&3 esa xk;ksa djuÝht rFkk lkghoky nksuksa esa xzh"e ½rq ds nkSjku ih&53 dk vijsxqys'ku FkkA xk;ksa Ýht dju rFkk lkghoky nkSjku ds lafèkdky FksA Ýht xk;ksa esa ch-,-,dl-@ch-lh-,y-&2 vuqikr vfèkdre iwoZC;kar rFkk i'pC;kWar nksuksa ds nkSjku lkghoky rFkk dju nkSjku ds ½rq xzh"e esa rqyuk dh ifjfLFkfr;ksa FkeksZU;wVªy mRiknu rFkk dksfVZlksy Lrjksa dks de djrk gSA rFkk pje tyok;qqoh; ifjfLFkfr;ksa ds nkSjku eqDr jsfMdy Lrj] vlaØkE;rk rFkk vkgkj vUr%xzZg.k esa lqèkkj djrk gS lkghoky cNM+ksa esa Øksfe;e izksik;ksusV dk lEiwj.k LokLFk~; Js"B Lohdk;Zrk bafxr djrh gSA dh xksi'kqvksa Fkkjikjdj vis{kk dh xksi'kqvksa Ýht dju (ih< mPp us thuksa mÙkjnk;h fy, ds ih-,e-bZ-,y-) rFkk (,e-lh-vkj- jatdrk Ropk nkSjku ds ½rq 'khr vis{kk dh ½rq xzh"e esa Fkkjikjdj (Vh-ih-) rFkk dju Ýht (ds-,e-) vksljksa nksuksa esa thuksa rFkk izksVhuksa dh igpkuA HkSal esa VªkalfØiVkse rFkk izksVhvkse }kjk Å"ek nkc ds lacaèk vfHkO;atuk iSVuZ vuqØfer dh xbZA ,p-,l-,Q ,l&1]24 ,p-,l-ih&79 rFkk xksi'kq ulyksa esa ij[kuyh QkbczksCykLV dksf'kdk ykbuksa esa fd;k x;kA ewY;kaadu djds iz;ksx dk rduhd ih-lh-vkj- ek=kRed dk izHkko ds nkc Å"ek ij QkbczksCykLV Roph; ds HkSal o`f¼ djus dk O;kid volj gSA xfrfofèk;ksa ds ekè;e ls fMvksfu xksi'kq esa iztuu izn'kZu dh vko';drkgSrFkkJs"Biks"kf.kd,oaiztuuizcUèku xksi'kq gS rFkk ,-,Q-lh- ,oa lh-vkbZ- ds fnuksa dks de djus fMvksfu ,d eè;e vkdkj dk] nksgjs mís'; okyk Lons'kh vkokl] leewY;rk] ½rqvksa vkfn ij fuHkZj djrk gSA tSls rRoksa xSj&vkuqokaf'kd ,ao vkuqoakf'kd fofHkUu ,e-) –– fnu ij egRoiw.kZ ksVksa ds HkSalksa eqjkZg Lrj dk dkjd fodkl raf=dk rFkk dkjd&1 fodkl tSls bUlqfyu dkjdksa] fodkl IykT+ek izHkko izfrdwy iM+kA eSuu vkfyxkslsdsjkbM ds lkFk vUrxZr gsIVkslkbV laoèkZuksa ds mipkj ls ds ifjfLFkfr;ksa gkbijFkfeZd xbZA ikbZ xzk-) ds lEiwj.k ls fu;a=.k oxZ dh rqyuk esa 0-01) FkkA 0-01) ik, x,A 1 eqjkZg HkSalksa ds dVM+ksa rFkk dVfM+;ksa 1-0 rFkk β xzk-)] ykbflu (60 L=o.k ij[kuyh esa o`f¼ esa ij[kuyh L=o.k 1-5 ih-ih-,e- Lrj ij 27 fu ds xzk-@fnu) xzk-) rFkk

¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ izkIr fd, x, rFkk Øe'k% ,Q vuqØe Vh- U;wDyksVkbM ,p- x,A ,Q fd, ,l] foLrkfjr Vkbi ehFksukstsul) rFkk vuqØfer U;wDyksVkbM budksfMax ,Q-Vh-,Q-,l- thul vkaf'kd (,slhVkstsul) }kjk iz;ksx dk fofèk ih-lh-vkj- FkkA ;qDr vkgkj ls mPp vkgkj {kerk rFkk vYi feFksu mRiknu cNM+ksa esa fuEu vif'k"V vkgkj vUrxzZg.k (vkj-,Q-vkbZ-) eè;e ,oa mPp vkj-,Q-vkbZ- oxks± dh rqyuk esa lkghoky jk'ku esa iz;ksx fd;k tk ldrk gSA ds i'kqvksa okys djus tqxkyh esa :i ds L=ksr ukbVªkstu ehFksu mRiknu esa lEiw.kZ deh ds lkFk lLrs fd.ou ;ksX; ij vkèkkj Mh-,u- fd x;k ik;k ;g x;kA ik;k izHkkoh essa djus de dks feFksu fcuk fd, izHkkfor dks iSjkehVjksa fd.ou :esu vU; Mkst ,u bu foVªks vè;;u ds vkèkkj ij czkseksDyksjksehFksu dh laHkkO;rk iznf'kZr dhA dh djus iz;ksx esa :i ds la;kstd vkgkj ls mi;ksx ds rRoksa iks"kd ls vkgkjksa fyXukslsY;qyksfld rFkk mRiknu nqXèk nSfud djus] izkIr Hkkj 'kjhj us fo;ksftr ls nzo vkgkj ij j[ks x, LFkk;h :i ls ukM+h of.kZr HkSalksa ds :esu okys js'ks mPp gSaA ekStwn ls :i izeq[k esa xksi'kqvksa ladj ,Q-Mh-&1 ds thokf.od laoZèku ij vkgkfjr dju Ýht ;qDr mPp js'ks okys vkgkj rFkk vkj- Qyso Hkwlh Qsflul LVªsu dh xsgWw QkbyksVkbi ehFksuksczfocsDVj fd gSa crkrs ifj.kke gSA gksrh tek esa Øeksa rhu fyh, ds djus iznku tkudkjh iwjh Js"B dh fofoèkrk Fkekstul esa :esu esa 16 ,lvkj vkj ,u , thu dh rqyuk esa vks-Vh-;w xksi'kqvksa ls feJ.k esa fNydk mrjs gq, fcukSys dh [kyh foyk;d lRo esa dh ykxr dks djus izkIr Hkkj 'kjhj fd-xzk- izfr esa cNfM+;ksa Ýht dju fd;k x;kA mÙkjnk;h fofu;ked izfØ;k dks vizR;{k :i ls ifjofrZr lqèkkj gqvk rFkkfi ekWal ,oa nwèk esa esa ikFkos lh-,y-,- p;kip; :esu lap;u ls ds vfèkfeJ.k ds fy, vEyksa olh; vo'kksf"kr ls iz;ksx ds Qkbcfjlksyoul C;wVªhfocfjvks Vkbi dk;ks± esa lqèkkj gqvkA ,Q-,- de djus esa ykHkizn izHkko gqvk ,u-bZ- rFkk ukbVªkstu] ftxj fd.ou ;wfj;k jDr rFkk gqvk lqèkkj esa ykHk tek fd, x,A 1571282 rFkk la[;k csufdV rFkk ckyk jkèkk] 10 Hkkx lks;kchu izfrLFkkfir djds fd;k tk ldrk gSA ,lvkj vkj ,u , thul (ehFksuks FkzksQl rFkk FkzksQl (ehFksuks thul , ,u vkj ,lvkj 16 II ehFksuksVªkQl ds fy, Dyksu uke vuqØe 15 Hkkx fcukSys dh [kyh vFkok ,d lkfUnzr 1571285 ds vUrxZr thu cSad MSVkcsl esa izfr'kr dsfYl;e ukbVªsV dsfYl;e izfr'kr 3 1570827] 1571273] 1] dq.Mw] I rFkk 4,u

¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ,d lkr vehuks vEy ;qDr isIVkbM (isIVkbM lh) (cksu) dksf'kdkvksa dks c<+krk gSA rFkk ftlesa ,- lh- b- dks ckfèkr djus dh {kerk gS eTTk ckfèkr djrk gSA isIVkbM lh tks fd ,d ,sVhavkDlhMsaV gS djds dksde ek=k dh dSFkiflu rFkk ,flM fjfllVuV VkjVjsV tSls% fpUgksa foHknsu vksfLV;ksDykLV rFkk djds de dks l[;k dh QkLQsVl ,flM fjfllVuV] VkjVjsV ls gksrk gSA iSIVkbM lh vksfLV;ksDykLV dh fØ;k'khyrk dks ,ydkbu QkLQsVl rFkk vksfLV;ksdSyflu dh vfèkd ek=k Hkh djrk gS] ftldk irk foHknsu fpUgksa dksytu Vkbi&1 vksfLV;ksCykLV dksf'kdkvksa dks c<+krk gS vkSj mudk foHksnu HkSal dsflu ls fudkys x, vkf.od vkèkkj ij izdk'k Mkyk x;kA ,aVªksdksdl ds 'kfDr izfrjksèk izfrdwy xbZA ds ihfMvksflu }kjk Qsdsfyl dh fodflr izfØ;k dh djus rS;kj vkS|ksfxd pht+ Ogs ls izHkkodkjh ikÅMj ;qDr ihfMvksflu nwèk ,oa nqXèk mRiknksa dh 'ksYQ ykbQ c<+kus esa iz;ksx ds fy, wk s RU , VqdM+s] igpku dh xbZA ,IVkelZ ds fo?kVu fu;rkad ,d fy, ds chVkdkslksekfQZu&7 mRiUu x,A fd, ls nwèk chVkdklksekfQZu&7 ds Lohdj.k fl¼kar ds izek.k l`ftr ,1 }kjk ,IVkejl LdhoM Vh ,p2 iSFkos lq>krk gSA pwgksa esa lqLi"V gS tks fd fu"dZ'kr% izkS<+koLFkk izkS<+ ds deh nkSjku ,d esa o`f¼ dh vlaØkE;rk f=nks"k&fo"kZ;d rFkk dksf'kdh; eè;LFk vlaØkE; izfrfØ;k] fpdkfyd iznkgd izksS<+ksa esa :X.krk rFkk eR;Zrk dks c<+kus dk izeq[k dkj.k gSA okys fodkjksa iznkgd rFkk jksxksa vlaØfer bE;wukslsus,Sl ch-,l-,e&7 x,A Fkkjikjdj) ds lhfer la[;k ds uewuksa esa vuqifLFkr ik, rFkk (lkghoky xksi'kqvksa ds uLyksa Lons'kh ,1 ysfdu x, ik, esa (ds-,Q-) fLol dju rFkk (ds-,Q-) Ýht dju β β vfèkd (0-2 ,e-th-@th xquk rhu vis{kk dh nwèk , ,1 fueqZfDr dh lh-,e-&7 ifjorZdksa ls ,1 thuksVkbiM dju Ýht ds nwèk ls ch- ,1 rFkk ,1 ls fo;ksftr dh xbZA izngu dks vfHkizsfjr djus esa izR;{k :i ls yxs gq, gSaA 7 dks vksjy bUV;wcs'ku us vkxs iqf"V dh fd ;s isIVkbM xV O;kolkf;d :i esa la'ysf"kr ch-lh-,e- nwèk dh rqyuk esa Vh-,p2 iSFkos ds ^ykbd* }kjk vfèkfefJr fd;kA ,2 dks izfrfØ;k bE;wu xV ds pwgksa vkgkj ds dslhu dks nksuksa ,1 rFkk 2 , vysy Lons'kh ladj xksi'kq dslhu ds , ^ykbd* (,Z ,2) (,Z ^ykbd* ,2 fd u gSa fueqZDr ls 2) β 1 ^ykbd* ifjofrZ;ksa (,1 , dslhu ds , ds dslhu β 7 vehuksa ,flM dk cuk iSIVkbM dslhu) FkhA ^ykbd* ifjofrZ;ksa (,1 ifjofrZ;ksa ^ykbd* 1 5 rFkk ch-lh-,e- ,IVkelZ dh ,IVkelZ 15 1 rFkk ,1 –– 7-0&156 fgUnh lkjka'k β dslhu ,e –– 2)

177 ndri annual report 2013-14 178 ndri annual report 2013-14 ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ –– ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ fgUnh lkjka'k djrs gSaA fpf=r ls :i Li"V dks vkØe.k ds ,iksiVksfll lfØ; ikFkos ds }kjk HkSalksa ds 'kqØk.kqvksa ds Øk;kslaj{k.k ds nkSjku ds rqyukRed fo'ys"k.k dklist+ fuHkZj ,oa dklist+ Lora= igyqvksa ,iksiVksfVd esa 'kqØk.kqvksa Øk;ksifjjf{kr ,oa rkts xbZA dh tkap dh la[;k thokf.od fof'k"V (cqcyl chp ds cqcsfyl) HkSalksa ty ,sMksesfVªfVd rFkk lkekU; i'pkr izlo fo'ys"k.k esVkthuksfed ds ekbØksck;ksVk xHkkZ'; p;kip; ekxZ ij izdk'k MkykA ifjofrZr }kjk nkc ÅtkZ esa xksi'kqvksa tscw us fo'ys"k.k ds ladsr VªkalfØiVksfed Y;wdkslkbVl jDr isjhQsjy oHU tf;s l nXk iks (3 miHkksx nqXèk ls tkfr;ksa fofHkUu esa vkU= fØ;k'khyrk ij ldkjkRed izHkko ns[ks x,A vkgkjh; lEiwj.k ls pwgksa esa mPp olh; vkgkj ifjfLFkfr;ksa ds nwèk fdf.or ;qDr dslh- ,y- (izksck;ksfVd) izfrtSoh; ,usykbVl ds lkUnz.k esa xgjk vuqiz;ksx gSa lkefxz;ksa dh muds fo'ys"k.k ds fy, vYi lkUnz.k esa ekStwn pqEcdh; uSukslkexzh dks iz;ksx'kkyk esa rS;kj fd;k x;kA bu ugha ik, x,A O;kolkf;d rS;kjh) dh vkgkjh; lekos'ku ls la;ksth izHkko ikÅMjksa (vè;;u esa iz;qDr ,yksohjk vo;ohl rFkk ftesusek flyosLVj dh vkS"kèkh; rFkkfi] gSA djrk bafxr dks laHkkO;rk dh ,y-jSeuksll) (fo'ks"kdj izfrtSoh;ksa esa jksdus dks o`f¼ dh LFkwyrk esa pwgksa vkgkj olk vPp lEiwj.k vkgkjh; ds vo;oksa vkS"kèkh; rFkk nwèk fdf.or (,y-VSeuksll@,y-IykusVje) (izfrtSoh;) izksck;ksfVd uhyk ls tkaph tkrh gSA ekU;rk lw{ed.kksa esa jax ds ifjorZu yky ls cSaxuh vFkok Lo.kZ lw{ed.kksa dh ijr dh vko';drk gksrh gS rFkk bldh fl¼kUr ds izek.k l`ftr fd, x,A fofèk Lohdj.k esa ds ,e1 ,IVkelZ vQykVkfDlu ds }kjk lkFk ,IVkelZ p;fur eku fu;af=r oxZ ds rqY; gSA izfrvkDlhdkjd {kerk mPprj gS rFkk cdjh ds nwèk dk gSA vU; oxks± dh rqyuk esa cdjh rFkk ÅWaV ds nwèk ds fy, ,Fkzkstsfud lwpdkad ,oa dksyLVªksy fu{ksi.èk dks de djrk h j d vo;r gsh S Fk lh ekU;rk bldh Lo.kZlw{ed.kksa esa jax rFkk ds ifjorZu ls tkaph gS tkrh gSA gksrh vko';drk dh ijr dh lw{ed.kksa Lo.kZ lkFk ds ,IVkelZ esa fofèk FksA chp ds ,e djrk gSA nwèk ds lkFk (3 o`f¼ esa Lrj ds dksyLVªksy mRlftZr ls ey vkgkj nqXèk ls tkfr;ksa fofHkUu gSA tkrk fn;k lkFk ds vkgkj Hkjiwj ls dksyLVªksy tc gS jksdrk dks o`f¼ esa Lrj DyksLVªksy –– % ) vkgkfjr lHkh oxks± esa ftxj esa % l) IykT+ek olk)

¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ;u ikfvskfd sVssll krka ,l tkfr;ksa (24&34)] ,l1&lh,u- ysDVkscsflyl izksfVvksykbfVd p;fur fuèkkZfjr dh xbZA olk rFkk lks;kchu rsy dh tkap ds fy,] fofHkUu fofèk;ka 'kkjhfjd&jklk;fud xq.kksa ds vkèkkj ij esa HkSal ds 'kjhj dh fo'ys"k.kkRed fofèk fodflr dh xbZA iqf"Vdj VksUM nwèk esa foVkfeu Mh2 ds vkdyu ds fy, ,d dh tkap ds fy, ,d fofèk fodflr dh xbZA vkj-ih-&,p-ih-Vh-,y-lh dk iz;ksx dj ?kh esa ouLifr rsy fLVªi vkèkkfjr ijh{k.k fodflr fd, x,A fy, ds tkap dh rVLFkhdkjdksa ,oa ;wfj;k fefJr esa nwèk fofHkUu ftaxj fd.od tSoekdZj gSA (usuksVkDlhflVh) ds dkj.k dksf'kdk {kfr ds l{ke ladsrd rqyuk esa rhu xquk ls Hkh vfèkd fo"kkDr FkkA lw{efo"kkDrrk dh ,u-,l-,u) (usuksikfVZdyl lw{ed.kksa eslksiksjl Vh-) (MCY;w-lh-,u- usuksV;wcl dkcZu (eYVhokYM) cgqfHkRrh; fnu rd lqjf{kr j[kk tk ldrk gSA Øk;ksifjjf{kr djuds ds fy, jsQjhtjsVj rkieku ij dks 'kqØk.kqvksa ds xk;ksa djuÝht rFkk HkSal ij foLrkjd fd;k x;k rFkk ijEijkxr :i ls iz;qDr vaMihrd ;qDr fodflr foLrkjd nwèk lks;k vkèkkfjr QkbVks ujohu rS;kj gsrq iz;ksx rqjUr esa :i lkfUnzr ij Lrj xzkeh.k ljapuk fuEu FkhA bU;wfyu ,oa izfrjksèkh LVkpZ ;qDr pht+ nksuksa dh

179 ndri annual report 2013-14 180 ndri annual report 2013-14 ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ –– ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ fgUnh lkjka'k izR;FkhZ vè;;uksa dk fo'ys"k.k djus ds fy, fd, x,A vfèkdre djus rFkk {ks= ds lQy y?kq Msjh Ï"kdksa ds p;fur dsl esa y?kqèkkjh Msjh mRiknu iz.kkfy;ksa dh ckèkkvksa dk vè;;u izksQkby vè;;u izkjaHk fd, x,A ;g vè;;u nf{k.k {ks= ij mRiknu Msjh y?kqèkkjh esa {ks= nf{k.k lkFk ds mís';ksa fof'k"V fy, ds djus vè;;u dk izksQkby dh iz.kkfy;ksa mRiknu Msjh èkkjd y?kq esa {ks=ksa p;fur ds {ks= nf{k.k fd, x,A dh vè;;u rqyukRed nwèk ij fo'ks"krkvksa lkfUnzr HkkSfrd&jklk;fud esa iz.kkyh gLrpkfyr ,oa ;a=hÏr laj{k.k vè;;u fd, x,A ÅtkZ nkSjku ds fuekZ.k [kksvk esa ,l-,l-,p-bZ- LVst Fkzh ;Vhel nè NMkZ kk) h kzr lksiZ'ku vknzZrk dh fo'ks"krkvksa dh tkap dh vkgkj) xbZA NqM+kbZ (nwèk iqf"Vdj tLrk U;wVªhfeDl ,oa ykSg vkèkkfjr ij cktjs ,oa nwèk fy, cyÏr laoguh fofèk fMtkbu ,oa fodflr dh xbZA bu ykbu iz.kkyh dk iz;ksx dj jcM+h ds Rofjr mRiknu ds x;kA ljQst Å"ek fofuek;d (,l-,l-,p-bZ-) la'kksfèkr fd;k LØsIM LVst Fkzh fy, ds mRiknu ds xktjikd fujUrj fy, e'khu foft+u iz.kkyh fodflr dh xbZZA jax ekiu ij vkèkkfjr Msjh mRiknksa ds xq.koRrk ewY;kadu ds dk Ldsy vi fMtkbu rS;kj fd;k x;kA lkfUnzr nqXèk lapkyu ds fy, fujUrj [kksvk fuekZ.k e'khu k=; r i nè ea zrhkk vfkVs d fy, ds chtk.kq&laonh vif'k"Vksa izkS|ksfxdh (fd.od) fodflr izfrthok.kq dh xbZA esa nwèk ij Lrj {ks=h; xbZA ekbØks osy ck;ksfpi vkèkkfjr tkap iz.kkfy;ka fodflr dh ,LlS rFkk muds tehZuksthfud lcLVªsV rFkk fefuV;qjkbT+M vkèkkfjr chtk.kq fy, ds tkap dh ,y-eksukslokbVkstsul dj iz;ksx dk fd.odksa ekdZj LisDVªe izfr lw{etSoh; fØ;kdyki iznf'kZr djrs gSaA Vªkal& isFkkstul rFkk jíh lw{e ouLifr;ksa dh rqyuk esa O;kid Fkkbeksy] cksuZ QwM vFkkZr~ us dkjokØksy rFkk b;wtuksy fluseyfMgkbM] la?kVd tSolfØ; ikni vkj-,l-2 dk iz;ksx fd;k tk ldrk gSA rFkk ,y-QjesUVe fy, bE;wukseksM~;wys'ku ds mipkj ds cpko] gkbijdksysLVªkyhfe;k ds laØe.k isFkkstu nqXèk mRiknu dh ykxr dh mRiknu nqXèk gSaA djrh gS rFkk fuf'pr vkuqokaf'kd vlekurk iznf'kZr djrs Msjh i'kqvksa (64&67 i'kqvksa Msjh 2&5 –– :i,@yhVj ds chp gSA chp ds :i,@yhVj 17&23 ekbØks&cSy IysV ij IysV ekbØks&cSy 96 % ) ds Lokeh gSaA {ks= esa {ks= gSaA Lokeh ds )

¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ f'k{k.k ,oa izf'k{k.k ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ efgyk Msjh lgdkjh lfefr;ksa dk la?kVukRed fo'ys"k.kA ij vFkZO;oLFkk dh ifjokjksa xzkeh.k esa {ks= iwohZ ds Hkkjr esa fyax Øksekslkse fuewZy ik;k x;kA iwohZ {ks=h; dsUnz esa tqM+oka ladj cNM+ksa&,d dsl vè;;u sh 'q kk ea q uhr vgj akkka dk lalkèkuksa rqyukRed iks"kf.kd vkgkj ewY;kadu ,oa mi;ksxA uohure dqN esa jk'ku i'kq Msjh fodkl fd;k x;kA fy, p;fur {kerk esa lqèkkj ds fy, vkuqokaf'kd ekMy dk if'peh caxky ij ladj xk;ksa ds nqXèk L=o.k fo'ks"kdksa ds jk"Vªh; Msjh vuqlaèkku laLFkku ds iwohZ {ks=h; dsUnz] dY;k.kh] mRiUu fd, x,A Hkzw.k ls dksf'kdkvksa LVSe Hkz.kh; ds xksi'kqvksa ls ij[kuyh dke pyk;k x;kA ls vkgkj ds fd.ou :esu dj iz;ksx dk iÙkksa ds isM+ksa ds cNM+ksa esa fodkl izn'kZu esa lqèkkj ds fy, mÙkjh&iwohZ Hkkjr eg zro ,-pt- v; fZ du rFkk djus vftZr l'kfDrdj.k dk vR;fèkd izHkkoh ekxZ vk; ik;k x;kA (,l-,p-th-) izLrko lewg ij izdk'k Mkyk x;kA egRoiw.kZ Hkwfedk ij cy nsus ds fy, uohu izoÙkuksa ds xq.kksa igpku dh xbZA rnuUrj esa Ms;fjax esa izkajfHkd izoÙkdksa dh dh rRoksa vfHkizsj.k rFkk fo'ks"krkvksa dh izoÙkdksa izkjafHkd iznku dh xbZA vFkZ'kkL= fo"k;ksa esa ekLVj mikfèk ds 'kksèkdk;Z gsrq lqfoèkk,a izkS|ksfxdh] i'kqèku mRiknu ,oa izcaèku] i'kq iks"k.k rFkk Msjh tSo i'kq ij dY;k.kh dsUnz] {ks=h; iwohZ izdkj blh x;kA mikfèk ds fy, Fkhfll dk;z djus ds fy, MkDVjsV esa mÙkjnkf;Ro vFkZ'kkL= lkSaik Msjh rFkk izkS|ksfxdh Msjh jlk;u] Msjh iztuu] i'kq rFkk fy, ds djus dk;Z 'kksèk esa fo"kZ; izkS|ksfxdh Msjh rFkk jlk;u Msjh fy, ds dk;ZØe mikfèk vofèk esa] Nk=ksa dks nf{k.k {ks=h; dsUnz] caxykSj ij ekLVj {ks=h; dsUnzksa ij f'k{k.k dks lqn`<+ fd;k x;k gSA fjiksVkZèkhu gSa buesa laLFkku esa ij Msjh izkS|ksfxdh esa fMIyksek ikB~;Øe Hkh izkjaHk fd;k gSA o"kZZ vfrfjDr us laLFkku ds izkS|ksfxdh) (Msjh ch-VSd rFkk mikfèk ikB~;Øeksa ,oa ekLVj pkyw ij fo"kZ;ksa fofHkUu esa laLFkku izkFkfedrk vkèkkj ij lqèkkjus dh vko';drk gSA lkeuk dj jgs gS tks fd Msjh dk fodkl ckèkkvksa lh dks cgqr jksd Ï"kd Msjh jgs fd gSa gS gksrk rFkk iznf'kZr ;g bUgsa 15 fons'kh Nk= Hkh lfEefyr gSaA 837 Nk= gSa ftuesa ls nf{k.k {ks=h; dsUnz] caxykSj dsUnz] {ks=h; nf{k.k ls 2013&14 645 yM+ds ,oa 192 yM+fd;ka ¹ ¹ ¹ ¹ ¹ ¹ foLrkj dk;Zdyki ¹ ¹ ¹ ¹ ¹ ¹ ij lQyrkiwoZd lkSais x,A Msjh foKku dkystksa dks vkWu ykbu rFkk vkQ ykbu eksM+ fodflr fd, x, rFkk jkT; Ïf"k fo'ofo|ky;ksa ds lHkh ds dk;ZØe izkS|ksfxdh) (Msjh ch-VSd egkfuns'kd] Hkkjrh; Ïf"k vuqlaèkku ifj"kn] ubZ fnYyh ubZ ifj"kn] vuqlaèkku Ïf"k Hkkjrh; egkfuns'kd] v¸;Iiu] ,l- Mk- rFkk gqvk laiUu O;k[;ku Lekjd }kjk fn;k x;kA rFkk fnukad Hkkjrh; fpfdRlk vuqlaèkku ifj"kn] ubZ fnYyh egkfuns'kd] Mk-oh-,l-dVksp] dks fy, ds o"kZZ fn;k bl O;k[;ku x;k eaMy] ubZ fnYyh }kjk fn;k x;kA Mk- ,u-,u-nLrwj Lekjd p;u oSKkfud Ïf"k lnL;] caèkksikè;k;] ,l-ds- Mk- dks Mk- ds-ds-vb;k Lekjd O;k[;ku fnukad djus ds fy, fn;k x;kA djus ds fy, izfrLi¼kZ ,oa izsj.kk dh Hkkouk dks izksRlkfgr ekLVj ,oa MkDVjy Nk=ksa dks mPp xq.koRrk dk 'kksèk dk;Z ds nwljs lIrkg rd euk;k x;kA ^Js"B Fkhfll iqjLdkj* Hkh 'kSf{kd ekl tuojh] x;kA fd;k vk;kstu dk xfrfofèk;ksa 'kS{kf.kd dbZ tSlh vkfn p;u dk Nk=ksa fy, ds 'kksèk&fucaèkksa MkDVjy ,oa fofHkUu izHkkxkè;{kksa }kjk f'k{k.k esa uoizoÙkZu] Js"B ekLVj rFkk izLrqrhdj.k dh miyfCèk;ksa 'kSf{kd rFkk O;k[;ku lekjksg ls iwoZ 'kSf{kd ekl euk;kA bl lIrkg esa Lekjd jk"Vªh; Msjh vuqlaèkku laLFkku us o"kZZ vk;qDr bl volj ij fof'k"V vfrfFk FksA i'kqikyu ,l-gksukikxksy] Mk- rFkk fnYyh ubZ ifj"kn] ikBd] miegkfuns'kd (i'kq foKku) Hkkjrh; Ïf"k vuqlaèkku esfjV izek.ki= rFkk iqjLdkj iznku fd,A Mk- ds-,e-,y- mn~cksèku izLrqr fd;k rFkk Nk=ksa dks mikfèk;ka] Lo.kZ ind] Hkkjrh; Ïf"k vuqlaèkku ifj"kn] ubZ lfpo fnYyh dkS'ky] vkj- us vjfoUn viuk Jh x;kA nh{kkUr fd;k vk;ksftr laLFkku dk ckjgoka nh{kkUr lekjksg nwljk pj.k Hkh iwjk dj fy;k x;k gSA dk djus fØ;kfUor dks ikB~;Øeksa bu fy, ds uohuhdj.k fujUrj rFkk iqujh{k.k gq, ns[krs dks QhMcSd fu;fer x;kA ikB~;Øeksa dk iqujh{k.k fd;k x;k rFkk bUgsa vfUre :i fn;k bu }kjk iqujh{kdksa Nk= rFkk fjO;wj ihj fy, ds fMyhojh Ïf"k fo'ofo|ky;ksa (,l-,-;w-) dks vkWu ykbu rFkk vkQ ykbu iwoZLukrd dk;ZØe izLrqr djus okys vU; laLFkkuksa rFkk jkT; gSA x;k fy;k dj iwjk bZ&ikB~;Øe fy, ds ikB~;Øe mikfèk ,u-,-vkbZ-ih-la?kVd xr ,d o"kZZ ds nkSjku fodflr izkS|ksfxfd;ksa ds gLrkarj.k viuk, x, xkWoksa esa Msjh Ï"kdksa rFkk Ï"kd efgykvksa ds fy, 11 Qjojh] 2014 ls nwljs lIrkg ls Qjojh 1 ds vUrxZr ch-VSd (Msjh izkS|ksxdh) izkS|ksxdh) (Msjh ch-VSd vUrxZr ds 2014 dks ikapok Mk- Mh- lqUnjslu 14 Qjojh] 2013&14 esa nh{kkUr 28 tuojh] Z ikB~;Øe bZ- 49 2014 dks 2014 2014

¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ us dqy us Qksu Ýh Vksy }kjk ds bZ-esy rFkk dkyl lkfgRo] izdkf'kr ppkZvksa] O;fDrxr ds jkT;ksa fofHkUu us dsUnz ,fVd ds laLFkku vuqlaèkku Msjh jk"Vªh; (ifjlj ij ,oa ifjlj ls ckgj) vk;ksftr fd,A ij igyqvksa fofHkUu ds Ms;fjax ,oa {ks=ksa lacafèkr ds Ïf"k tSls foKku x`g ikyu] eRL; ds fgr ds fy, Qly mRiknu ckxokuh] eèkqeD[kh ikyu] 6873 Ï"kdksa] xzkeh.k efgykvksa] xzkeh.k ;qokvksa ,oa ds m|fe;ksa ns'k iwjs us dsUnz izf'k{k.k Msjh rFkk dsUnz foKku Ïf"k tkudkjh izkIr dhA esa ckjs ds lqfoèkkvksa miyCèk ij laLFkku ,oa miyfCèk;ksa us laLFkku ij gks jgs fofHkUu vuqlaèkku] f'k{k.k rFkk izlkj 6121 vkxUrqdksa (Nk= ,oa ladk;) us Hkze.k fd;kA bu lewgksa esa laLFkku iz;ksxA ls iznku djuk rFkk Ïf=e xHkkZèkku (,-vkbZ-) dk O;kid dh {kerk dk fuekZ.k fd;kA i'kqfpfdRlk lsokvksa dks rhozrk rFkk i'kqfpfdRlk] izkjafHkd mipkj esa csjkstxkj xzkeh.k ;qokvksa ih-Mh- xHkkZèkku] Ïf=e ikyu] Msjh }kjk i¼fr djuky ftys ds us ykHk mBk;kA ftlls x, fd, vk;ksftr dk;ZØe laLFkku ifjlj ij pkj efgyk m|ferk ,oa {kerk fuekZ.k loafèkZr Msjh mRiknksa dks y?kqLrj ij mRiknu djus ewY; ds gSA fy, ldrh j[k dk;e okrkoj.k LoLFk esa ifjokjksa vius&vius rFkk gSa ldrh dj vftZr vk; vfèkd ls 511 xzkeh.k efgykvksa us ykHk mBk;ka xzkeh.k efgyk,a Ms;fjax l'kfDrdj.k izf'k{k.k ,oa esa f'kfoj vk;ksftr xkWaoksa fd, ckgj x,A ds blls ifjlj fy, ds efgykvksa tkx:d fd;k x;kA Hkh esa ckjs ds mRiknju ds pkjs gjs o"kZZ iwjs rFkk izcaèku dk pkjs esa vofèk dh deh dh pkjk djuk] rS;kj mRikn ekinaM] LoPN nqXèk mRiknu izfØ;kvksa] ewY; loafèkZr Msjh cpko jksx fu;a=.k] FkuSyk izfØ;kvksa izcaèku iztuu dks fy, ds la;qDr :i ls ,d cht fcØh dsUnz izkjaHk fd;k x;k gSA Ïf"k fo'ofo|ky; yqfèk;ku }kjk laLFkku ds ,fVd dsUnz ij ds fy, igyh ckj jk"Vªh; Msjh vuqlaèkku laLFkku rFkk iatkc ( dEiksLV ( `

@&) rFkk chtksa dh fcØh] ,Q-okbZ-,e-] oehZ oehZ ,Q-okbZ-,e-] fcØh] dh chtksa rFkk 1]62]379@&) ` fdlku laxksf"B;ka vk;ksftr dh xbZA Ï"kdksa xbZA dh vk;ksftr laxksf"B;ka fdlku 53

` 6 O;fDr;ksa dks ohfM;ks 'kks] O;k[;kuksa] O;k[;kuksa] 'kks] ohfM;ks dks O;fDr;ksa 1]54]790

4]82]685@&) }kjk vftZr fd;kA Ï"kdksa ds fgr ] dkystksa@laLFkkuksa@fo'ofo|ky;ksa ls dqy ls dkystksa@laLFkkuksa@fo'ofo|ky;ksa 104 45] @& dk jktLo] iqLrdksa dh fcØh }kjk fcØh dh iqLrdksa jktLo] dk 064@& 6 CykWdksa esa fy, x, lsok,a iznku dhA dsUnz dsUnz dhA iznku lsok,a 8802 z'{. dk;ZØe izf'k{k.k 230 xzkeh.k efgykvksa xzkeh.k 64 12 xkWoksa esa oSKkfud –– fgUnh lkjka'k efgyk 34 ––

181 ndri annual report 2013-14 182 ndri annual report 2013-14 ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ vkèkkjh; lajpuk –– ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ fgUnh lkjka'k bZ-daVSaV viyksfMax ds fy, lajfpr gSA vku ykbu fMfyojh ds fy, bZ-daVSUV l`tu] la'kksèku ,oa rFkk gS lqlfTtr lkFk ds midj.kksa vko';d lHkh ;g xbZA dh laLFkkfir iz;ksx'kkyk fodkl bZ-daVSUV esa izHkkx rFkk okYd bu dksYM :e izkIr fd, x,A f'kad&jSi iSdsftax e'khu] Qyksa dk xwnk fudkyus dh e'khu fQfyax e'khu] lCth izlaLdj.k e'khu] LisDVªksQksVksehVj] Ýhtj] vkblØhe vkblØhe ÝwV QhMj] IysuVjh feJd] csdjh vksou] loZ di lkQV ykbu] dSfuax e'khu] iSfdax Hkjus] cksrysa u, e'khu] fuekZ.k ikLrk esa tSls midj.k la[;k dkQh vUrxZr ds ifj;kstuk ch-ih-Mh- kjk; è;ka s y ikèu kZ s O;ogkj esa ekuhVfjax iz.kkyh Hkh QkeZ laLFkkfir dh xbZA i'kqèku fy, ds vè;;uksa vkpj.kh; ,oa lqlfTtr fd;k x;kA ok;jySl laosnh vkèkkfjr tyok;q fu;a=.k d{k fodflr zky] a;Vhr qè nsu zky rk i'kq rFkk iz.kkyh fØ;kdyki nksgu vuqJo.k iz.kkyh izkjaHk ,oa ewY;kafdr dh xbZA nqXèk daI;wVjhÏr fMLiSaflax iz.kkyh] daI;wVjhÏr iz.kkfy;ksa] vkokl rhu gsrq vR;kèkqfud dY;k.k i'kq izHkkoh ,oa vkjke dss xk;ksa Msjh lqfoèkk,a ,oa vU; ewy vuqlaèkku lqfoèkk,a lfEefyr gSaA vks-2 bUD;wcsVj vkfn) fgLVksyksth lqfoèkk,a] izksfVvksfeDl (tSo lqj{kk dsfcusV] bUojfVM Qy~jkslSaV ekbØksLdksi] lh- LFkkfir dh xbZ gSA lqfoèkkvksa esa dksf'kdk laoèkZu lqfoèkk,a laLFkku ij ,d vR;kèkqfud ^Fkhfj;ksthuksyksth vuqlaèkku iz;ksx'kkyk* Msjh ,u-,Q-ch-,l-,Q- jk"Vªh; vUrxZr ds ifj;kstuk ,-vkj-,- iz;ksx'kkyk% ^Fkhfj;ksthuksyksth* fodkl (ch-ih-Mh-) ,dd l`ftr fd;k x;k gSA ,oa fu;kstu O;olk; uohu vUrxZr ds ,u-,-vkbZ-ih- Qhft;ks&ftuksfed iz;ksx'kkyk LFkkfir dh rFkk xbZ gSA QsuksVkbi p;kip; fy, ds vuqlaèkku izeq[k ,oa vxz.kh esa i'kqfokku x;kA i'kqèku fd;k LFkkfir dsUnz okyh vuqlaèkku j[kus dk;e dks ifjorZuksa tyok;qoh; ;qDr ls lqfoèkkvksa dh iz.kkyh vfHkys[ku O;ogkj rFkk fØ;kRed 'kjhj 'kSYVj] i'kq fMtkbuM dLVe pSEcj] i'kq dysjksehVj] lh-vks- vk;ksftr djus ds fy, dkQh mi;ksxh gksxhA dk;Z fodkl fodkl@ekud lalkèku fodkl@ekuo ,oa gSA ;g iz;ksx'kkyk ,Q-,l-,l-,-vkbZ- ds fy, vuqlaèkku xbZ dh l`ftr iz;ksx'kkyk* ^jsQjy ,d fy, ds lqj{kk ,oa xq.koRrk ^nqXèk ij laLFkku vuqlaèkku Msjh jk"Vªh; –– 2 i;kZoj.k pSEcj] lkbØksehfVªd ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ djus dk izLrkoA ds dadjhV Q'kks± ds lqèkkj ,oa Hkwfe fodkl dks dk;kZfUor jk"Vªh; Msjh vuqlaèkku laLFkku ds iwohZ {ks=h; dsUnz] dY;k.kh ij i'kq'kkyk dksjhMksj dh [email protected] jk"Vªh; Msjh vuqlaèkku laLFkku ds iwohZ {ks=h; dsUnz] dY;k.kh esa eSVy lM+dksa dh dkjisfVax ,oa uohdj.kA jk"Vªh; Msjh vuqlaèkku laLFkku ds iwohZ {ks=h; dsUnz] dY;k.kh ij pkjnhokjh dh ejEerA jk"Vªh; Msjh vuqlaèkku laLFkku ds iwohZ {ks=h; dsUnz] dY;k.kh Nk=ksa ds Nk=kokl ds ^ch rFkk bZ-* foax dk uohdj.kA ij caxykSj n-{ksds] ds laLFkku vuqlaèkku Msjh jk"Vªh; k i lLku k k csZ xu ,a apk dk uohdj.kA lajpuk ,oa yxkuk cksMZ uke dk laLFkku ij }kj eq[; laLFkku ds caxykSj laLFkku] vuqlaèkku Msjh jk"Vªh; Mh-lqUnjslu Lekjd lHkkHkou dh ejEer ,oa uohdj.kA d{k@lfefr d{k ds dejksa dh ejEer ,oa uohdj.kA lsehukj ds vuqHkkx QkeZ ifjlj dk;kZy; rFkk odZ'kki QSal dh ejEer (8 ds CykWd ua- fofHkUu 'kSM+ksa ,oa dejksa dh ejEer ,oa uohdj.k rFkk QkeZ {ks= ijh{kk dsUnz ds :i esa ifjofrZr fd;k x;kA Msjh bathfu;fjax izHkkx ds ;wVhfyVh vuqHkkx dks laLFkku ds dk fuekZ.kA lqfoèkkvksa uohu dh d{kksa gky@cSBd lEesyu vfrfjDr vUrjjk"Vªh; Nk=kokl esa dkQh ejEer dk;ZA lHkh Nk=koklksa esa bUVjusV lqfoèkk,a fodflr dh xbZA vkWu&ykbu ,-,l-vkj-ch- ijh{kk dsUnz pkyw fd;k x;kA pkjk laxzgky; l`ftr fd;k x;kA jk"Vªh; Msjh vuqlaèkku laLFkku ij yxHkx dejs ,dy gSA esa Nk=kokl bl Nk=ksa ds fy, u;k Nk=kokl ^ueZnk Nk=kokl* cuk;k x;kA cuk, x,A lHkh dejksa ds lkFk ckFk:e Hkh cus gq, gSaA fd;k x;A dqy dkosjh Nk=kokl dk u;k foax l`ftr fd;k x;k rFkk pkyw laoèkZu iz;ksx'kkyk Hkh laLFkkfir dh xbZ gSA ÅÙkd ,d vfrfjDr blds rFkk gS x;k fd;k lqlfTtr dks uohure :i nsus ds fy, bls vR;kèkqfud lqfoèkkvksa ls Msjh lw{etho foKku izHkkx dh vkf.od tho foKku ,dd

7 esa 'kSM ,oa laxzg.k lqfoèkk iznku djuk] ekStwnk 52 desj&(,dy dejs) yM+fd;ksa ds fy, Nk=ksa ds jgus dh O;oLFkk gSA lHkh gSA O;oLFkk dh jgus ds Nk=ksa 150 *ÅpkbZ) rd CykWd ua-

6 esa rkj yxkukA 2 ,dM+ Hkwfe ij

¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ x,A fd, forfjr izek.ki= dks izfrHkkfx;ksa ,oa iqjLdkj udn dks fotsrkvksa ds izfr;ksfxrkvksa vk;ksftr nkSjku ds ekl uoEcj] dk;ZØe forj.k iqjLdkj ,oa lekjksg eq[; jktHkk"kk us Hkkx fy;kA dk;Z'kkyk vk;ksftr dh xbZ ftlesa laLFkku ds rFkk fVIi.k vfrfjDr blds x,A fd, iznf'kZr iksLVj* ^'kksèki=@ ,d gsrq Nk=ksa 'kksèk ,oa vfèkdkfj;ksa rduhdh oSKkfudksa 'kqHkkjaHk dk ekl bl x;kA fd;k vk;kstu dk lfefr ds lnL; dk;kZy;ksa gsrq fnukad fnukad izfr;ksfxrk ys[ku elkSnk ,oa fVIi.k gsrq dkfeZdksa ds oxks± lHkh ds laLFkku ls mís'; ds c<+kus i=kpkj nSfud x;kA elkSnk ys[ku ,oa Hkk"k.k izfr;ksfxrk dk vk;kstu Hkh fd;k flrEcj] fnukad Hkh ckj bl Hkkafr dh o"kks± foxr esa laLFkku ofj- ,dd] jktHkk"kk izHkkjh] (jktHkk"kk)@iz'kklu mifuns'kd (iz'kklu) funs'kd la;qDr ls vksj dh laLFkku esa cSBdks bu xbZA dh vk;ksftr esa vè;{krk dh 'kekZ Mh-ds- Mk- dsUnzh; e`nk yo.krk vuqlaèkku laLFkku esa ogka ds funs'kd ekl esa laiUu gqbZ ,oa nwljh N%ekgh cSBd fnlEcj ekl esa twu cSBd leh{kk N%ekgh igyh dh lfefr dk;kZUo;u laLFkku ds funs'kd egksn; dh vè;{krk esa uxj jktHkk"kk gsrq fnukad gsrq fyfidksa ofj"B ,oa fyfidksa ds laLFkku vfrfjDr blds dk;ZØe esa dk;ZØe bl x;kA fd;k vk;kstu dk izfr;ksfxrk xhr&xk;u dks djus dk iz;kl fd;k tkrk gSA fd, tkus okys dk;ks± dh :i js[kk rS;kj dj mUgsa fØ;kfUor dk;ks± dh izxfr dk voyksdu fd;k tkrk gS rFkk Hkfo"; esa esa jktHkk"kk dk;kZUo;u ds {ks= esa laLFkku }kjk fd, tk jgs pkj esa cSBdksa bu xbZA dh vk;ksftr cSBd frekgh izR;sd vFkkZr o"kZ dh lfefr dk;kZUo;u jktHkk"kk laLFkku rd- 21-92013 dks laiUu gqbZA uxj jktHkk"kk dk;kZUo;u dks vk;ksftr fd;k x;k ftlesa jktHkk"kk ftlesa x;k fd;k vk;ksftr dks 2013 ls 2013 vfèkdkjh jktHkk"kk ,dd us Hkkx fy;kA flrEcj] 28 dk;kZy;ksa ds dk;kZy;ksa 18 uoEcj] 11 dks jktHkk"kk iz'kklfud jktHkk"kk dks 2013 dkfeZdksa us Hkkx fy;kA Hkkx us dkfeZdksa 39 rd jktHkk"kk ekl jktHkk"kk rd 2013 25 flrEcj] jktHkk"kk dk;Zdky ( 69 dkfeZdksa jktHkk"kk ,dd 2013 11 17

¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ ¹ 2013&14 ds lkStU; ls vk;ksftr fd;k x;kA Hkkx fy;kA ;g dk;ZØe dsUnzh; vuqokn C;wjks] ubZ fnYyh fofHkUu dk;kZy;ksa ds yxHkx ftlesa x;k fd;k vk;ksftr dk;ZØe izf'k{k.k vuqokn fnukad esa rRokèkku ds lfefr dk;kZUo;u jktHkk"kk uxj esa laLFkku }kjk fd;k tkrk gSA ,dd bl dk;Z vuqokn dk izdkj vusd ,oa O;k[;ku Kkiu bR;kfn fofHkUu lekjksgksa dh izsl foKfIr;ka] Hkk"k.k] ds 'kksèk lkjka'k] okf"kZd izfrosnu] iz'kklfud i=@ifji=@ laLFkku ds oSKkfudksa ls oSKkfud ,oa yksdfiz; ys[k] Nk=ksa esa dke&dkt c<+sA ewY;kafdr dj iqjLÏr fd;k tkrk gS rkfd jktHkk"kk (fganh) ewy :i ls fyf[kr ,oa izdkf'kr ys[k@vkys[k@'kksèki=ksa dks }kjk muds esa if=dkvksa fofHkUu }kjk oSKkfudksa vUrxZr ds ;kstuk izksRlkgu bl gS pkyw esa laLFkku ls o"kks± dbZ foxr ;kstuk izksRlkgu ys[ku ys[k oSKkfud fganh ewy rd igqap ldsA yksxks vfèkd ls vfèkd Kku bldk ftlls gS Hkstrs gsrq ys[k jktHkk"kk (fganh) esa fy[k dj bl if=dk ls izdkf'kr fy, vR;Ur mi;ksxh gSA oSKkfud vius fo"k; ls lacafèkr lQy izdk'ku gqvkA ;g izdk'ku Ï"kdksa ,oa i'kqikydksa ds laLFkku dh okf"kZd x`g if=dk ^nqXèk xaxk* ds r`rh; vad dk iqjLdkj jktHkk"kk f}rh; eè; ds dk;Zdykiksa gsrq djuky fLFkr yxHkx mYys[kuh; lacaèkh dk;kZUo;u jktHkk"kk jgs tk fd, esa laLFkku }kjk djuky lfefr] dk;kZUo;u jktHkk"kk uxj o"kZ vUrxZr ds ;kstuk izksRlkgu ys[ku elkSnk ,oa fVIi.k esa fganh ls :i ewy 26-062013 dks iznku fd;k x;kA dkfeZdksa dks iqjLÏr fd;k x;kA d d kp nl; laf{kIr fnolh; ikap ,d rd 19-72013 ) ds fy, laLFkku ds laLFkku fy, ds 2012&13 35 dkfeZdksa ,oa vfèkdkfj;ksa us 66 dsUnzh; dk;kZy;ksa fnukad 2012&13 09

183 ndri annual report 2013-14 184 ndri annual report 2013-14