309 Identification of estradiol/ERa-regulated genes in the mouse pituitary Hyun Joon Kim1,2,*, Mary C Gieske1,3,*, Kourtney L Trudgen1,*, Susan Hudgins-Spivey1, Beob Gyun Kim4, Andree Krust5,6, Pierre Chambon5,6, Jae-Wook Jeong7, Eric Blalock8 and CheMyong Ko1,3 1Division of Reproductive Sciences, Department of Clinical Sciences, University of Kentucky, Lexington, Kentucky 40536, USA 2Department of Anatomy and Neurobiology, Institute of Health Sciences, Medical Research Center for Neural Dysfunction, School of Medicine, Gyeongsang National University, Jinju, Korea 3Department of Biology, University of Kentucky, Lexington, Kentucky 40536, USA 4Department of Animal Science and Environment, Konkuk University, Seoul 143-701, Korea 5Institut de Genetique et de Biologie Moleculaire et Cellulaire (CNRS, INSERM, ULP, College de France), 67404 Illkirch Cedex, Strasbourg, France 6Institut Clinique de la Souris, BP10142, 67404 Illkirch Cedex, Strasbourg, France 7Department of Obstetrics, Gynecology and Reproductive Biology, Michigan State University College of Human Medicine, Grand Rapids, Michigan 49503, USA 8Department of Molecular and Biomedical Pharmacology, University of Kentucky, Lexington, Kentucky 40536, USA (Correspondence should be addressed to C Ko who is now at Division of Clinical and Reproductive Sciences, College of Health Sciences, University of Kentucky, Lexington, Kentucky 40536, USA; Email:
[email protected]) *(H J Kim, M C Gieske, and K L Trudgen contributed equally to this work) Abstract Estrogen acts to prime the pituitary prior to the GnRH- pathway in the pituitary. This approach substantiates ERa induced LH surge by undiscovered mechanisms. This study regulation of membrane potential regulators and intracellular aimed to identify the key components that mediate estrogen vesicle transporters, among others, but not the basic action in priming the pituitary.