Product Datasheet

Gemtuzumab Antibody (975155) [Unconjugated] MAB96171-SP

Unit Size: 25 ug Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Updated 10/23/2017 v.20.1

Earn rewards for product reviews and publications. Submit a publication at www.novusbio.com/publications Submit a review at www.novusbio.com/reviews/destination/MAB96171 Page 1 of 3 v.20.1 Updated 10/23/2017 MAB96171-SP Gemtuzumab Antibody (975155) [Unconjugated] Product Information Unit Size 25 ug Concentration Please see the vial label for concentration. If unlisted please contact technical services. Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. Clonality Monoclonal Clone 975155 Reconstitution Instructions Reconstitute at 0.5 mg/mL in sterile PBS. Isotype IgG2a Conjugate Unconjugated Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS. Product Description Host Mouse Species Multi-Species Specificity/Sensitivity Detects Gemtuzumab in Western blots and direct ELISAs. No cross-reactivity with Cetuximab, Ibritumomab, Rituximab, Adalimumab, Tositumomab, Brentuximab, Trastuzumab, Solanezumab, Teprotumumab, and Aducanumab is observed. Immunogen Full length Gemtuzumab Notes This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand. Product Application Details Applications Western Blot, ELISA, Flow Cytometry Recommended Dilutions Western Blot 0.5 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, ELISA

Images Western blot shows Rituximab,Trastuzumab, Gemtuzumab, and Brentuximab biosimilars. PVDF membrane was probed with 0.5 µg/mL of Anti-Gemtuzumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB96171) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Gemtuzumab at approximately 240 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1. Page 2 of 3 v.20.1 Updated 10/23/2017 U937 Human histiocytic lymphoma cell line was stained with anti-Siglec- 3/CD33 (Gemtuzumab) (Catalog # MAB9635), then Anti-Gemtuzumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB96171, filled histogram) or Mouse IgG2A Isotype Control Antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti- Mouse IgG Secondary Antibody (Catalog # F0101B).

A panel of biosimilars was coated at 1 μg/mL in 96-well polystyrene plates. Anti-Gemtuzumab (Anti-Idiotype) Antibody (Catalog # MAB96171, 333 ng/mL) detected Gemtuzumab. No cross-reactivity with Cetuximab, Ibritumomab, Rituximab, Adalimumab, Tositumomab, Brentuximab, Trastuzumab, Solanezumab, Teprotumumab, and Aducanumab was observed.

Biotinylated biosimilars were serially diluted and incubated with Anti- Gemtuzumab (Anti-Idiotype) Antibody (Catalog # MAB96171, green line) coated at 1 μg/mL on a 96-well polystyrene plate. No cross-reactivity with Rituximab (black line) or biotinylated IgG control (orange line) was observed.

The anti-Idiotype antibody (catalog number MAB96171) directed against Gemtuzumab was coated at 4 μg/mL in a 96-well polystyrene plate to capture the biosimilar Gemtuzumab. Biotinylated Anti-Gemtuzumab used at 0.5 μg/mLwas used as the detection antibody before incubation with Streptavidin-HRP. Other biosimilars did not bridge the anti-idotype antibody, showing specificity of the anti-Gemtuzumab (anti-Idiotype) antibody. Novus Biologicals USA Novus Biologicals Canada 8100 Southpark Way, A-8 461 North Service Road West, Unit B37 Littleton, CO 80120 Oakville, ON L6M 2V5 USA Canada Phone: 303.730.1950 Phone: 905.827.6400 Toll Free: 1.888.506.6887 Toll Free: 855.668.8722 Fax: 303.730.1966 Fax: 905.827.6402 [email protected] [email protected]

Novus Biologicals Europe General Contact Information 19 Barton Lane www.novusbio.com Abingdon Science Park Technical Support: [email protected] Abingdon, OX14 3NB, United Kingdom Orders: [email protected] Phone: (44) (0) 1235 529449 General: [email protected] Free Phone: 0800 37 34 15 Fax: (44) (0) 1235 533420 [email protected]

Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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