Dr Santosh Kumar Sahu Photo

Dr Santosh Kumar Sahu

Important web-links ORCID: https://orcid.org/0000-0002-5141-8103 Research gate: https://www.researchgate.net/profile/Santosh_Sahu15 Google scholar: https://scholar.google.com/citations?user=pZjZk-IAAAAJ&hl=en Photo

1. Name: Santosh Kumar Sahu 2. Designation: Assistant professor 3. Qualification: M.Sc. (Zool) (UU), PhD (Biotech) (IITM), Post Doc 1(ATP-independent Flippases in ER) (Weill Cornell Medical College, Cornell University, New York, USA), Post Doc 2 (Serotonin Receptor), (Center for Cellular and Molecular Biology, Hyderabad, India) 4. Phone: 09178939281 5. E.mail: [email protected] 6. Area of Interest: Biochemistry (With special reference to membrane biology) 7. Courses Taught: Biochemistry, Cell biology, Recombinant DNA technology, Enzymology 8. Research Guidance  M.Phil Submitted: 05, Ongoing: 01

Student name and Submitted/ Subject Guide/ Thesis Title Date of Roll No. Ongoing Co-guide submission Jesus Ray Submitted Zoology Co-guide Biochemical studies on phospholipase activity 05/05/2016 R.N. :13001N15008 of total venom from eastern Indian viper (Daboia russelli) Susmita Pati Submitted Biotechnology Co-guide Screening of Anticancer activity potential of 26/09/2016 R.N.:M16101N15003 Selected Medicinal Plants of Odisha and Evaluation of their Cytotoxic Potential on Malignant Cells Sibani Pal Submitted Biotechnology Guide Production of industrially important enzymes 01/09/2017 R.N.:M16101N16003 from mercury tolerant soil bacteria Rashmi Shee Submitted Biotechnology Guide Evaluation of the antimicrobial potential of 31/08/2018 R.N:M16101N170010 Nyctanthes arbor-tristis and Gymnema sylvetre against heavy metal and membrane mediated drug resistance in bacteria Upasana Sahoo Submitted Biotechnology Guide Characterization of phospholipase activity in a 16/08/2019 R.N: M16101N18009 Hg2+-tolerant Klebsiella pneumonaie Swastisarita Shaw Ongoing Biotechnology Guide Flippase-mediated bacterial drug resistance 2020 R.N: 19MPhil BT503 (class)  Phd Submitted: 01, (Ongoing): 03

Student and PhD Subject Broad Title of Synopsis Date of Regn number registration Sushrirekha Das Submitted Zoology Isolation and characterization of probiotic lactic acid 02/02/2016 Regn No. (Co-guide) bacteria and evaluation of their nutritional potential Sc./Zool/01/PhD/2016 for iron and folic acid utilization using animal model system Himadri G. Behuria Ongoing Biotechnology Stimulation of scramblase mediated apoptosis in 22/06/2016 (INSPIRE fellow, (Guide) malignant cells using indigenous phytochemicals of DST, Govt. of India) Odisha Regn No. Sc/BT/01/PhD/2016 Sangam Gupta Ongoing Biotechnology Purification and Characterization of Mercuric 22/06/2016 Regn No: (Guide) reductase (MerA) from Mercury resistant bacterial Sc/BT/05/PhD/2016 strain Gayatree Panda Ongoing Biotechnology Investigating the Mechanism of Multiple Peptide 12/12/2018 (INSPIRE fellow, (Guide) Resistance Factor (Lysyl-PG flippase)-mediated DST, Govt. of India) Antibiotic Resistance in S. aureus and Screening of Regn No its Phytochemical Inhibitors Sc/BT/11/PhD/2018

9. Publications

(I) JOURNAL

(A) INTERNATIONAL

 H.G. Behuria and S.K. Sahu, An anti-microbial terpenoid fraction from Gymnema sylvestre induces flip-flop of fluorescent-phospholipid analogs in model membrane, Applied Biochemistry and Biotechnology, 2020, IF: 2.3, DOI: https://doi.org/10.1007/s12010-020-03399-3, ISSN: 0273-2289 (Print) 1559-0291 (Online)

 H. G. Behuria, B. K. Biswal & S. K. Sahu, Electroformation of liposomes and phytosomes using copper electrode, Journal of Liposome Research, 2020, IF: 2.5, DOI: https://doi.org/10.1080/08982104.2020.1800729, Print ISSN: 0898-2104, Online ISSN: 1532-2394

 S. Pati, S. K. Sahu, S. Mohapatra, P. R. Debata, H. N. Thatoi, Screening and evaluation of anticancer activity of phytochemicals from three selected medicinal plants, (2019) 94399-JAS-ANSI (in press)

 S.K. Sahu, H. G. Behuria, S. Gupta, Copper and Iron Overload Protect E. coli from Exogenous H2O2 by Modulating Membrane Phospholipid Composition. Environment Sustainbility, (2019) 2, 23-32, ISSN: 2523-8922, DOI: https://doi.org/10.1007/s42398-019-00046-4

 S.K. Sahu, H. G. Behuria, Biochemical Characterization of H2O2-Induced Oxidative Stress in E.coli. Journal of Applied Microbiology and Biochemistry, 2 (2018) 3:10, ISSN 2576-1412, DOI: 10.21767/2576-1412.100026

 H. G. Behuria, R. Shee, B.K. Biswal S. Gupta, S.K. Sahu, Evaluation of Biotechnological Potential of Novel Mercury Tolerant Strain of Klebsiella Pneumonia. Journal of applied Microbiology and Biochemistry, 2 (2018) 1-7, ISSN 2576-1412, DOI: 10.21767/2576-1412.100027

 S.K. Sahu, R. B. Dalua, S. Sahoo, D. Parida, S. Ghosh, S. Padhi, S. Gupta, H. G. Behuria, Cobalt- Induced Cytotoxicity in E. coli (DH5α) is Mediated by Modulation of Cellular Phospholipid Composition, Journal of advanced microbiology, 5 (2016) 215-223. ISSN Online: 2349-7785

 S.K. Sahu, H. G. Behuria, S. Gupta, R. B. Dalua, S. Sahoo, D. Parida, S. Ghosh, S. Padhi, Biochemical Characterization of High Mercury Tolerance in a Pseudomonas Spp. Isolated from Industrial Effluent, American Journal of Current Microbiology 4 (2016) 41-54, ISSN Online: 2572-5815

 D. Nandi, S.R. Mishra, C.K. Sahu, S.K. Sahu, Ground water quality in the industrial belts of Balasore town, Odisha, International journal of earth and atmospheric sciences (2015): 2, 16-19, ISSN:2349-9222

 S.K. Sahu, H. G. Behuria, S. Gupta, B. Sahoo, Sequence analysis of a subset of plasma membrane proteins containing CXXC metal binding motif, International Journal of Knowledge Discovery in Bioinformatics (IJKDB) 5 (2015) 1-15. (10995), ISSN (printed): 1947-9115, https://hdoi.org/10.4018/IJKDB.2015070101

 S. K. Sahu, R. Saxena, A. Chattopadhyay, Cholesterol depletion modulates detergent resistant fraction of in plasma membrane leads to reduction of serotonin1A receptor in membrane, Molecular Membrane Biology 29 (2012) 290-298. (17890), IF: 1.647, ISSN : 0968-7688 (print), https://doi.org/10.3109/09687688.2012.688147

 B. Sahoo, S. K. Sahu, T. Swarnkar Prediction of sub-cellular localization of scramblase protein family, International Journal of Computer & Communication Technology (IJCCT) for (2010) 79-87. ISSN (PRINT): 0975 – 7449 [Special Issue of IJCCT Vol.1 Issue 2, 3, 4; 2010 for International Conference [ACCTA-2010], 3-5 August 2010]

 S. K. Sahu, A. Rajasekharan, S.N. Gummadi, GroES and GroEL are essential chaperones for folding of recombinant human phospholipid scramblase1 in E. coli, Biotechnology Letters 31 (2009) 1745-52. (3106), IF 2.154, ISSN: 0141-5492, https://doi.org/10.1007/s10529-009-0073-7

 S. K. Sahu, S. N. Gummadi, A. Gopalakrishna, Calcium binding studies of peptides of human phospholipid scramblases 1 to 4 suggest that scramblases are new class of calcium binding proteins in the cell, Biochemica et Biophysica Acta 1790 (2009) 1274-128. (2741), IF: 3.67, ISSN: ISSN 0304- 4165, https://doi.org/10.1016/j.bbagen.2009.06.008

 S. K. Sahu and S. N. Gummadi, Flippase activity in proteoliposomes reconstituted with Spinacea oleracea endoplasmic reticulum membrane proteins: evidence for biogenic membrane flippase in plants, Biochemistry 47 (2008) 10481-10490. (2884), ISSN: 0006-2960, IF: 2.952, https://doi.org/10.1021/bi8014339

 S. K. Sahu, S. N. Gummadi, A. Gopalakrishna, Over-expression of recombinant human phospholipid scramblase 1 in E. coli and its purification from inclusion bodies, Biotechnology Letters 37 (2008) 2131- 2137. (3106), IF 2.154, ISSN: 0141-5492, DOI: https://doi.org/10.1007/s10529-008-9797-z

 S. K. Sahu, S. N. Gummadi, N. Manoj, G. K. Aradhyam, Scramblases: an over view, Archives of Biochemistry and Biophysics 462 (2007) 103-114. IF:3.5, (2022), ISSN:0003-9861, DOI: https://doi.org/10.1016/j.abb.2007.04.002

(B) NATIONAL

 E. coli (DH5α) responds to H2O2-induced toxicity by modulating cellular phospholipid composition. S.K. Sahu, H. G. Behuria, S. Sahoo, S. H. Panda and N. Mohanty, H2O2-Induced Cytotoxicity in E. coli (DH5α) alters Cellular Phospholipid Composition, NOU J. Sc. & Tech.(in press)

(II) Books/ Book Chapters

(A) INTERNATIONAL

 S. N. Gummadi, D. S. Kumar, S. S. Dash, S. K. Sahu, Industrially important carbohydrate degrading enzymes from yeasts: pectinases, chitinases and β-1,3-glucanases, Yeast biotechnology: diversity and applications, DOI: 10.1007/978-1-4020-8292-4_30, Springer Netherlands, Dordrecht,, (2009), Chapter 30, Online ISBN: 978-1- 4020-8292-4, print ISBN:978-1-4020-8291-7, p. 673-691.

 S.K. Sahu, Science, society and evolution of scientific attitude in human, Alumni Meet-2017, DOI: 10.28921/nrme.sc.1.2017.120, New Madras Education, Pvt., Ltd., ISBN: 978-81-934044-6-1, pp: 11- 16

 H.G. Behuria, N.S. Pal, R.N. Munda and S.K. Sahu, Preparation of giant unilammelar vesicles (GUVs) from bacterial polar lipid extract: Developing a prokaryotic model membrane system, Biotechnology for sustainable utilization of Bioresources, Astral International Pvt. Ltd, New Delhi, 2019, ISBN:978- 93-88982-00-9 (HB), Chapter 28, pp 309-320

 H.G. Behuria, G. Panda and S.K. Sahu, Evaluation of antimicrobial potential of Nyctanthes arbortristis leaf extracts, Biodiversity Conservation and Livelihood Management, Springers (In press)

 S.K. Sahu and H.G. Behuria, Heavy metal toxicity and their bioremediation using microbes, plants and Nano-biomaterials, Springers (in press)

 H.G. Behuria, G. Panda and S. K. Sahu, Electroformation of Giant Unilamellar Vesicles from Bacterial polar Lipid Extract using Copper Electrode, Green Technology for Environmental Management (In press)

10. SEMINARS/CONFERENCES AND WORKSHOPS

S.N. Authors/presenters/ Title Name of Conference/Workshop Organizing institute Month Year speaker (Dates) 1 S.K. Sahu short term course under short term course under continuing department of 19th-20th 2005 continuing education programme education programme on Biotechnology, Indian September on "MAMMALIAN CELL "MAMMALIAN CELL CULTURE" Institute of Technology CULTURE" S.K. Sahu, S.N. Flippases and Scramblases of  Osmania University, th th 2 INTERNATIONAL CONGRESS ON 6 to 8 2008 Gummadi and A. plasma membrane BIOPROCESSES IN FOOD Hyderabad Nov Gopalakrishna, INDUSTRIES (ICBFI, 2008) th  th th 3 S.K. Sahu, S.N. Mechanism of Phospholipid  77 Annual meeting of SOCIETY OF IIT, Madras, Chennai  18 -20 2008 Gummadi and A. Scrambling in plasma membrane BIOLOGICAL CHEMISTS (India) Dec Gopalakrishna, 4 S.K. Sahu  Flippases in Plasma membrane Keck Biomembrane Retreat  Cornell University, 8th-10th 2010 Ithaca, New York, USA. June 5 B. Sahoo, S. K.  Prediction of sub-cellular International Conference on Advances  CV Raman College, June 2010 Sahu, T. Swarnkar localization of scramblase in Computer, Communication IEEE, Bhubaneswar, protein family, International Technology and Applications 1 Odisha Journal of Computer & Communication Technology (IJCCT) for (2010) 79-87. 6 S. K. Sahu, R.  Cholesterol depletion modulates XXXV All India Cell Biology National Institute of (16th to 18th 2011 Saxena, A. detergent resistant fraction of in Conference (AICB) on membrane Science Education and Dec. Chattopadhyay, plasma membrane leads to dynamics and disease, Research, Bhubaneswar, reduction of serotonin1A receptor Odisha in membrane 7 S.K. Sahu  Stimulation of Malignant Cell- 4th international conference on stem Haffkine Institute, 19th to 22nd 2013 Specific Apoptotic Pathways by cells and cancer: proliferation, Mumbai, India Oct Targeting Plasma Membrane differentiation and apoptosis Microdomains 8 S.K. Sahu (speaker) E.coli (DH5α) responds to 2nd International conference on Frontiers National Institute of 22nd- 2015 and H.G. Behuria oxidative stress by modulating in Biological Sciences (InCoFIBS) Technology, Rourkela, 24th/Jan cellular phospholipid Odisha, India, composition.  9 S.K. Sahu, N.B. Kar, Bacteria respond to iron-stress National Seminar on current trends of Department of Zoology, 19th -20th 2014 S.H. Panda, H.G. by depleting phospholipid animal science research in India, North Orissa University March, . Behuria, S.R. Behera, content in plasma membrane N. Dewangan, S. Mohapatra, P. Nayak, N. Mohanty 10 S. K. Sahu*, H.G.  Biochemical Characterization of Challenges and Practices of Biodiversity Department of Zoology, 20th ‐21st 2015 2+ Behuria, R.B. Dalua, Hg - induced Oxidative Stress Conservation With Special Reference to North Orissa University, November, S. Sahoo, D. Parida, Response in Bacteria Isolated Herpetofauna Odisha, India from Hg2+-enriched industrial S.Ghosh and S.Padhi Effluents 11 S. K. Sahu*, H.G. Hg2+-induced Oxidative Stress National Seminar on Science and Indian Science Congress 9th to 11th 2015 Behuria, R.B. Dalua, Response in Bacteria is mediated Technology for Indigenous Association, December S. Sahoo, D. Parida, by Alteration of Cellular Development in India Bhubaneswar Chapter S.Ghosh, S.Padhi Phospholipid Composition and KIIT University,

Odisha, India 12 S.Gupta, R.B. Dalua, Co2+-induced Cytotoxicity alters National Seminar on Science and Indian Science Congress 9th to 11th 2015 S. Sahoo, D. Parida, Cellular Phospholipid Technology for Indigenous Association, December S.Ghosh, S.Padhi, S. Composition in E.coli (DH5α) Development in India Bhubaneswar Chapter  K. Sahu* and KIIT University, Odisha, India 13 S. K. Sahu*, P. Biodiversity of Hg2+-tolerant National seminar on frontiers of Department of Wild Life February 2016 Mohapatra, A. K. bacteria in industrial effluents of Biodiversity and Remote Sensing, Rout, R.K. Sahu Odisha North Orissa University 14 S. K. Sahu* “Experimental Approaches  to One day National Symposium on Proteomics Society of 02-03-2016 2016 Proteomics” Proteomics India, Institute of Life Science, Bhubaneswar, Odisha, India 15 S. Das, S.H. Panda, Purification, characterization 57th Annual conference of association of Gauhati University, 24-27, 2016 S.K. Sahu, D.R.Das, and optimization of bacteriocin microbiologists of India and Assam, India Nov-2016 N. Mohanty by Lactobacillus rhamnosus and International symposium on microbes its application as biopreservative and Biosphere: What’s new What’ next in sprouts ? 16 S. Gupta and S.K. Molecular characterization  of National conference, Bhubaneswar KIIT, Bhubaneswer, 11-12, Dec, 2016 2+ Sahu some Hg resistant soil bacteria chapter Odisha, India 2016 isolated from industrial effluents 17 H.G. Behuria and Preparation of Giant Unilamellar National conference, Bhubaneswar KIIT, Bhubaneswer, 11-12, Dec, 2016 S.K. Sahu Vesicles (GUV) from Bacterial chapter Odisha, India 2016 Lipid Extract: Developing a Model Membrane System for Prokaryotes 18 S.K. Sahu Work shop on hands on training Three Days hands on training workshop P.G. Department of 8TH -10TH 2017 in Molecular biology and organized by Himedia Laboratories Pvt Biotechnology, North November immunology techniques” Ltd Orissa University, (National) Baripada, Odisha 19 H.G. Behuria and Preparation of Giant Unilamellar National Seminar on Challenges and Department of Physics, 23-24 Feb 2018 S.K. Sahu Vesicles from Bacterial Polar prospects in Physics North Orissa University 2018 Lipid extract 20 S.K. Sahu Purification and Characterization Recent Trends in Microbiology and MITS, BBSR, ODISHA 16-17 2018 of a Mercury-Sensitive Alkaline Biotechnology (RTMB-2018) March, 2+ Phosphatase from a Hg - 2018 tolerant Soil Bacterium 21 S.K. Sahu Biochemical Adaptation and  20th Odisha Bigyan “O” paribesh NISER, BBSR, Jatni 17-18 Nov 2018 Biotechnological Potential of a Congress 2018 Mercury Tolerant K. pneumoniae 22 S.K. Sahu Membrane-Mediated Drug  National Seminar on climate change and Department of Zoology, 22-23 Dec 2018 Resistance in Bacteria future of animals Prananath Autonomous 2018 college, Khordha in Collaboration with ZSO and P.G. Department of Zoology, Utkal University 23 S.K. Sahu Biological model membrane: an Multidisciplinary National Seth Phoolchand 29-30 Jan 2019 emerging tool in Basic and Conference on “Impact of Emerging Agrawal Smriti 2019 applied life science Science and Technology on Society: Mahavidyalya, Opportunity and Challenges” Nawapara-Rajim,  Raipur, Chhattisgarh, India 24 S.K. Sahu Biological Model Membranes:  Naional conference on advances in life Department of Life 27-28, Feb 2020 (Young scientist An emerging tool in Basic and sciences and Biotechnology: Life tech science and Depertment 2020 lecture) Applied Life Science 2020 of Biotechnology, Rama Devi Women’s University, Vidya Vihar, Bhubaneswar 25 S.K. Sahu Molecular mechanism of Corona National Webinar on “Recent Trends in Shri Muktanand College, 18-May, 2020 (Resource person) Virus infection and it’s Virology: COVID-19” Gangapur, Aurangabad, 2020 preventive measures Maharastra, India- 431109

11. Projects/ Consultancy

S.N Title Funding Sanction letter Duration Amount Agency Number & Date 1 Purification and Characterization of DST, Govt. 1191/ST-(Bio)-02/2017 3 years 8.51 Lakhs mercuric reductase (Mer A) from highly of Odisha Dated: 01/03/2017 mercury resistant, biofilm forming bacterial strains isolated from mercury- enriched industrial wastes of Odisha

12. Professional experience

S.N. Position held Dates Thrust area of University/Institute research 4 Assistant 05/07/2012 to present Biochemistry  Department of Biotechnology, North Orissa professor (Membrane biology) University, Takatpur, Baripada, Odisha, India. 3 Post doctoral 31.03.2012 to 04.07.2012 Membrane biology P.G. Department of Zoology, Utkal University, research (RA, DST-PURSE ) Vanivihar, Bhubaneswar. 2 Post doctoral 01/01/ 2011 to 30.06.2011 Membrane biology  Center for Cellular and Molecular Biology, research (RA in JC Bose Project ) Hyderabad, India. 01.07.2011 to 30. 03. 2012 (DBT-RA) 1 Post doctoral 15/03/2010 to 31/12/2010 Biochemistry Weill Cornell Medical College, Cornell research (membrane biology) University, New York, USA.

13. Ongoing Research Activities

(A) Brief over view of ongoing research activities

Plasma membrane is the boundary that separates the living interior of a cell from its non-living exterior. However, it is not a passive structure, rather a highly flexible, dynamic entity that regulates multiple cellular functions. For example it determines (1) diffusion of metabolites across itself, (2) regulates lipid-protein interaction, (3) secretion of molecules and (4) entry of pathogens. It plays an active role in (5) entry of drugs and (6) cellular response to antimicrobial agents. Bacteria as well as eukaryotes respond to change in their environments by altering lipid and protein composition as well as organization in their membrane. Many antimicrobial drugs exhibit their bactericidal or bacteriostatic activity by changing their membrane organization and composition that in turn decreases membrane stability, leading to cell lysis. My research interest merges biochemistry, cell biology, membrane biology, microbiology, enzymology, nanotechnology and environmental biotechnology for sustainable utilization of indigenous bioresources for their therapeutic potential. To list a few of them are (i) The biotechnological potential of membranotropic anti-microbials/anti-cancer compounds from indigenous natural resources (e.g. medicinal plants), (ii) Design of liposomes and phytosomes for drug delivery into microbes as well as mammalian cells, (iii) Investigate the flippase and scramblase-mediated mechanism of drug resistance in multi-drug resistant bacteria, (iv) Utilize the natural flippase-inducing compounds for enhanced delivery of therapeutics into target cells.

ALKP

A brief overview of the research work performed in my laboratory. (I) Giant unilamellar vesicles (GUVs) prepared using copper electrodes is a model membrane for studying the membrane-mediated activities such as membranotropic phytochemicals (II) Purification of industrially important proteins from bacteria, (III) Stimulation of cardiolipin-domain formation by high (200 µM) Hg2+ content on the membrane of K. pneumoniae, (IV) High Hg2+-concentration in growth medium increases the resistance of a K. pneumoniae isolate (Genbank ID: MK639927.1) (https://www.ncbi.nlm.nih.gov/nuccore/MK639927.1) isolated from Hg2+-rich industrial effluents, (V) High alkaline phosphatase (ALKP) activity in a Hg2+-tolerant Klebsiella pneumoniae, (VI) Flow diagram showing purification of a CHCl3-extracted terpenoid fraction from Gymnema sylvestrae (CETFGS) that exhibits flippase activity in giant unilamellar vesicles

14. COURSES/TRAINING PROGRAMMES ATTENDED

Name of the Subject Organizer Duration of Grade Obtained Course/Programme course 6th Orientation NA Human Resource Development 25/07/2016 to A programme Center, UGC, JNU 19/08/2016 Refresher course 1 Physics with special Human Resource Development 25-05-2017 to 14- A reference to Nano- Center (HRDC), ISM (IIT) 06-2017 Biotechnology Dhanbad Refresher course 2 Life sciences Human Resource Development 15-02-2019 to 07- A Center (HRDC), HCU, Hyderabad 03-2019 15. ACCOLADES / SCHOLARSHIPS/FELLOWSHIPS

 Prized for outstanding service as seminar secretary in during M.Sc in P.G. Department of Zoology, Utkal University (2004)  Prized for standing 2nd in departmental english essay competition during M.Sc. (2003) in Department of Zoology, Utkal University. st  Prized for standing 1 in state science quiz competition during M.Sc. (2003).  Joint CSIR-UGC Junior Research Fellowship (JRF) and Eligibility for Lectureship-National Eligibility Test (NET) , December 2003 and June 2004.  Joint CSIR-UGC Junior Research Fellowship (JRF) and Eligibility for Lectureship-National Eligibility Test (NET), June 2004.  Research Associate-ship at Weill Cornell Medical College, NewYork, USA-  JC Bose fellowship at Center for Cellular and Molecular Biology, Hyderabad, India  DBT-Research Associate-ship, DBT-India, July, 2011.

16. INVITED TALKS/RESOURCE PERSON

1. S.K. Sahu, Phospholipid scramblases, Sept 2011, P.G. Department of Zoology, Ravenshaw University, Odisha 2. S.K. Sahu, Phospholipid scramblases: emerging roles and significance, National seminar on cell science, June 2012, P.G. Department of Zoology, Utkal University, Odisha 3. S.K. Sahu, Heavy Metal Toxicity: Impact on Human Health and Environment with Special reference to Mercury, Sept, 2015, NOU connect, North Orissa University, Odisha 4. S.K. Sahu, Role of phospholipid scramblases in malignancy Oct 13, 2016, MPC Autonomous college, North Orissa University, Odisha 5. S.K. Sahu, “Cancer Biology” Jan 13, 2019, MPC Autonomous college, North Orissa University, Odisha

17. INNOVATIONS IN TEACHING METHDS/E-LEARNING/ONLINE TEACHING VIDEOS

(A) MEMBRANE BIOLOGY

S.N. Type of teaching Subjects to which the video is Access link on internet innovation useful 1 Teaching video in Cell Bio, Mol Bio, Biochemistry, http://youtu.be/vRRBsfAuR7k social media Lipidomics, Membrane biology 2 Teaching video in Cell Bio, Mol Bio, Biochemistry, http://youtu.be/kWdbZKB37fc social media Membrane biology

(B) ENZYMOLOGY Subjects to which the video is useful Access link on internet Properties and Kinetics of enzymes: 1. https://www.youtube.com/watch?v=Ea58_-D5Blc General characteristics of enzymes, 2. https://www.youtube.com/watch?v=d0jD8c9g_sI Nomenclature and Classification of 3. https://www.youtube.com/watch?v=EOLGFWu-v_w 4. https://www.youtube.com/watch?v=A9VYB3wFtEQ enzymes. Common enzymes (Biology of 5. https://www.youtube.com/watch?v=L9f4n2WatAU lysozyme, RNAseA, Chymotrypsin, 6. https://www.youtube.com/watch?v=TOtXqaifZ7w glutathione reductase and carbonic 7. https://www.youtube.com/watch?v=cu04nsGhkUw https://www.youtube.com/watch?v=ZAib92oC_sY anhydrase), Isozymes and their biological 8. 9. https://www.youtube.com/watch?v=NruaWQfzkr4 significance prosthetic groups, concept of 10. https://www.youtube.com/watch?v=bNVgUWBzRRw enzyme-substrate interaction. Forces of 11. https://www.youtube.com/watch?v=5A7F86vfO_U enzyme-substrate interaction 12. https://www.youtube.com/watch?v=EXpLhoKUaFU&t=15s 13. https://www.youtube.com/watch?v=0AWGikfy1Pk 14. https://www.youtube.com/watch?v=Kt0dfFc2keA Biology of lysozyme, RNAseA, 15. https://www.youtube.com/watch?v=Fsayq_wlG1Y Chymotrypsin, glutathione reductase and 16. https://www.youtube.com/watch?v=PnfYrdbZ0U4 carbonic anhydrase 17. https://www.youtube.com/watch?v=B56fIzpl52o 18. https://www.youtube.com/watch?v=lhnIu0UbLbg Isozymes and their biological significance 19. https://www.youtube.com/watch?v=fkfRBeqeSa0 concept of enzyme-substrate interaction 20. https://www.youtube.com/watch?v=ZAib92oC_sY 21. https://www.youtube.com/watch?v=U1__bK42NV0 Forces of enzyme-substrate interaction 22. https://www.youtube.com/watch?v=ZAib92oC_sY 23. https://www.youtube.com/watch?v=U1__bK42NV0 Rate of an enzymatic reaction (Rate 24. https://www.youtube.com/watch?v=N2Y8CFKCNcg determinants and rate constants), 25. https://www.youtube.com/watch?v=4spAz3hYJDI Michaelis-Menten equation 26. https://www.youtube.com/watch?v=akgSEO08TYI 27. https://www.youtube.com/watch?v=4p3q9s1i3yE 28. https://www.youtube.com/watch?v=lmSFTao3aMA 29. https://www.youtube.com/watch?v=ztPpZWlRVzI Determination of Vmax and Km of an 30. https://www.youtube.com/watch?v=0yrIPyKEens enzyme from Line-Weaver-Burk plot Enzyme inhibition 31. https://www.youtube.com/watch?v=qtSHgTyUTD4 32. https://www.youtube.com/watch?v=9Pnd6bRcqA0 Reversible inhibition 33. https://www.youtube.com/watch?v=qtSHgTyUTD4 34. https://www.youtube.com/watch?v=9Pnd6bRcqA0 Competitive inhibition 35. https://www.youtube.com/watch?v=leJ2cM3yXxs Uncompetitive inhibition 36. https://www.youtube.com/watch?v=plhgfudl_NU Mixed inhibition 37. https://www.youtube.com/watch?v=F4lwSOSBNSs Non-competitive inhibition 38. https://www.youtube.com/watch?v=Q-UgeFllg1M Irreversible inhibition 39. https://www.youtube.com/watch?v=XdG-JxNQCdY Non-Suicidal inhibition 40. https://www.youtube.com/watch?v=d24hKwF9gEU&t=19s Suicidal inhibition 41. https://www.youtube.com/watch?v=jWMe6ZoBscQ&t=39s 42. https://www.youtube.com/watch?v=dc5fVVxcq0Y Laws of thermodynamics, Energy 43. https://www.youtube.com/watch?v=Vjv996ukJRE diagram of enzyme catalyzed reaction, 44. https://www.youtube.com/watch?v=sFgvcU4rhCA Enthalpy and Entropy changes in 45. https://www.youtube.com/watch?v=ziwCDZXNRXM 46. https://www.youtube.com/watch?v=G0uB5pldUeo enzymatic reactions 47. https://www.youtube.com/watch?v=TllXoMVmCTw 48. https://www.youtube.com/watch?v=Z3YQP9MwpuQ 49. https://www.youtube.com/watch?v=U1__bK42NV0 Basic enzymatic reactions and their 50. https://www.youtube.com/watch?v=_7oPurE1VMs mechanisms, Mechanism of biochemical 51. https://www.youtube.com/watch?v=NdSOenEHnRY reactions 52. https://www.youtube.com/watch?v=U1__bK42NV0 Details of the mechanism of action for 53. https://www.youtube.com/watch?v=PnfYrdbZ0U4 Lysozyme, 54. https://www.youtube.com/watch?v=Y5-JCBPap1E 55. https://www.youtube.com/watch?v=GZPiH2_aAi8 Chymotrypsin 56. https://www.youtube.com/watch?v=Fsayq_wlG1Y 57. https://www.youtube.com/watch?v=25lB-luKlk0 58. https://www.youtube.com/watch?v=FKj6kOkfbQM 59. https://www.youtube.com/watch?v=zbOCy0IU1pA Glutathione Reductase 60. https://www.youtube.com/watch?v=lhnIu0UbLbg 61. https://www.youtube.com/watch?v=FlFftHlpWgA Carbonic anhydrase 62. https://www.youtube.com/watch?v=B56fIzpl52o 63. https://www.youtube.com/watch?v=0iz1W3lObxE Allosteric regulation of enzymes: 64. https://www.youtube.com/watch?v=YghX1jQXheg characteristics of allosteric regulation 65. https://www.youtube.com/watch?v=YwL6Npnpq2U (regulation of aspartate transcarbamoylase Covalent modification of enzymes 66. https://www.youtube.com/watch?v=gxhqKi3cAjk (regulation of protein phosphatase and 67. https://www.youtube.com/watch?v=il8zdoRLgow protein kinase A Regulation by proteolytic cleavage 68. https://www.youtube.com/watch?v=-WyOPWcO5JY (chymotrypsinogen) Regulation by isoenzymes 69. https://www.youtube.com/watch?v=fkfRBeqeSa0

18. MEMBER IN PROFESSIONAL BODIES

S.N. Professional body Member Duration 1 Proteomics Society of India Life member (Regd No. 326) 15/03/2016 to present

(19)

Students currently working in my laboratory

(20)

21. P.G. DISSERTATIONS GUIDED

S.N. STUDENT NAME/ROLL NO THESIS TITLE UNIVERSITY YEAR 1 Shibani Mohapatra DNA fingerprinting of new plant-type rice (NPT) (Oryza sativa) North Orissa 2013 Roll No 16101N114013 using SSR microsatellite marker University 2 Jyotirupa Das Effect of high Fe3+ on Mn2+ sensitivity of a Pseudomonas sp North Orissa 2013 Roll No. 16101N114005 University 3 Subhada P. Mohanty Effect of high iron concentration on membrane phospholipids North Orissa 2013 Roll No. 16101N114012 University 4 Sutapa Dandapat Effect of high iron concentration on total phospholipid content of a North Orissa 2013 Roll No 16101N114004 Pseudomonas sp. Isolated from iron-rich industrial effluent University 5 Chandan Hembram Production of siderophores by Pseudomonas sp. in iron-stressed North Orissa 2013 Roll No. 16101N114007 medium University 3+ 6 Subhasmita Saho Effect of high Fe on H2O2-sensitivity of Pseudomonas species North Orissa 2013 Roll No 16101N114020 University 7 Barsha Karmakar Effect of High iron concentration on regulation of intracellular iron North Orissa 2013 16101N114338 in Pseudomonas sp University 8 Himadri Gourav Behuria Mechanism of phospholipid-mediated signalling in E. coli (DH5α) North Orissa 2014 16101N124003 to survive oxidative stress University 9 Smruti Ranjan Behera Modulation of Phosholipid content in three different soil bacteria North Orissa 2014 Roll No 16101N124002 in response to oxidative stress University 10 Sasmita Mohapatra Regulation of iron homeostasis in three different soil bacteria North Orissa 2014 Roll No 16101N124015 University 11 Priranki Nayak Overexpression of proteins in response to oxidative stress in three North Orissa 2014 1610N12406 different bacteria isolated from Barbil iron mines University 12 Neha Dewangan Moderate iron protects against oxidative stress in a bacterium North Orissa 2014 16101N124006 isolated from iron-rich soil University

13 Susmita Sahoo Mechanism of H2O2-induced toxicity in E. coli (DH5α): North Orissa 2015 16101N134016 Biochemical characterization of lipid-mediated cytotoxicity in University bacteria 2+ 14 Subhasmita Padhi Effect of Cu on H2O2-induced toxicity in E. coli (DH5 α): a North Orissa 2015 Roll No. 16101N134011 biochemical study University

15 Debendra Parida Effect of CoCl2 n oxidative modification of lipids in E. coli (DH5 North Orissa 2015 16101N134012 α): mechanism of Co-mediated toxicity in bacteria University

16 Simarani Ghosh Effect of iron on H2O2-induced oxidative stress in E. coli (DH5α): North Orissa 2015 Roll No 16101N134005 Biochemical studies on mechanism of lipid-mediated oxidative University stress in bacteria 17 Pankaj Ranjan Mohapatra Alteration of plasma membrane organisation in bacteria induced North Orissa 2016 Roll No 16101N144010 by mercury toxicity University 18 Rakesh kumar Sahoo Isolation and Biochemical characterization of mercury-resistant North Orissa 2016 16101N144019 bacteria isolated from mercury-rich industrial effluents University 19 Balaram Naik Alkaline phosphatase activity in some mercury resistant bacterial North Orissa 2016 16101N144012 strain isolated from industrial effluents University 20 Asish Kumar Rout Mechanism of oxidative stress resistance in some mercury tolerant North Orissa 2016 16101N144016 bacteria isolated from industrial effluents University 21 Sonali Prava Dasmohapatra Screening of mercury tolerant bacterial strains from heavy metal- North Orissa 2017 Roll No 16101N154004 enriched soil in water samples and characterization of their University mercury reductase activity 22 Saswati Nanda Isolation and characterization of alkaline phosphatase-producing North Orissa 2017 Roll No 16101N154013 bacteria from soil samples of industrial area University 23 Baisali Dalabehera Evaluation of amylase activity in bacteria from soil and water North Orissa 2017 Roll No 16101N154003 samples of Industrial wastes to isolate potential amylase-producing University strain 24 Suryasnata Behera Study of membrane-mediated cytotoxicity by using GUV as a North Orissa 2018 16101N164003 model membrane against phytochemicals extracted from University Nyctanthes arbortristis 25 Milli Rani Das Purification and characterization of antimicrobials from North Orissa 2018 16101N164005 phytochemicals extracts of Nyctanthes arbortristis University 26 Vandana Swain Biochemical characterization of Alkaline phosphatise from K. North Orissa 2018 16101N164019 pneumonaie University 27 Manmath Rout Evaluation of the potential antimicrobial acivity of phytochemicals North Orissa 2018 Roll No 16101N164017 extracted from Gymnema Sylvestrae and Nyctanthes arbortristis University 28 Suprava S. Dash Evaluation of antibiotic resistance in a mercury tolerant K. North Orissa 2019 Roll No 16101N174005 pneumonaie University 29 Sonali Mohapatra Characterization of MerA activity in a mercury tolerant K. North Orissa 2019 16101N174014 Pneumonaie isolate University 30 Dayanidhi Sahoo Purification and characterization of bioactive compounds from North Orissa 2019 16101N174024 Gymnema sylvestrae University 31 Deepika Mohapatra Purification and characterization of antimicrobial principle from North Orissa 2019 16101N174016 chloroform extract of Gymnema sylvestrae University 32 Pranabkanti Dutta Membrane lipid modifications and its effect on Drug resistance in North Orissa 2020 16101N184008 S. aureus (Review only) University 33 Subhasis Satapathy Resistance of Klebsiella pneumoniae to β-lactam antibiotics North Orissa 2020 16101N184026 (Review only) University 34 Sukumar Rout Antimicrobial studies on Gymnema sylvestre (Review only) North Orissa 2020 16101N184023 University 35 Kshanaprava Mohanta Synthesis of green liposomal nanoparticles (Review only) North Orissa 2020 16101N184014 University 36 Kaustuv Padhi Membranolytic Potential of Nyctanthes arbor-tristis (Review only) North Orissa 2020 16101N184018 University