PPEF1 monoclonal antibody (M01), clone 1F6-1A5

Catalog # : H00005475-M01 規格 : [ 100 ug ] List All

Specification Application Image

Product Mouse monoclonal antibody raised against a full length recombinant Western Blot (Transfected lysate) Description: PPEF1.

Immunogen: PPEF1 (AAH36026, 1 a.a. ~ 653 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Sequence: MGCSSSSTKTRRSDTSLRAALIIQNWYRGYKARLKARQHYALTIFQSIEY ADEQGQMQLSTFFSFMLENYTHIHKEELELRNQSLESEQDMRDRWDYV DSIDVPDSYNGPRLQFPLTCTDIDLLLEAFKEQQILHAHYVLEVLFETKKV enlarge LKQMPNFTHIQTSPSKEVTICGDLHGKLDDLFLIFYKNGLPSERNPYVFNG Western Blot (Recombinant DFVDRGKNSIEILMILCVSFLVYPNDLHLNRGNHEDFMMNLRYGFTKEILH protein) KYKLHGKRILQILEEFYAWLPIGTIVDNEILVIHGGISETTDLNLLHRVERNK MKSVLIPPTETNRDHDTDSKHNKVGVTFNAHGRIKTNGSPTEHLTEHEWE ELISA QIIDILWSDPRGKNGCFPNTCRGGGCYFGPDVTSKILNKYQLKMLIRSHE CKPEGYEICHDGKVVTIFSASNYYEEGSNRGAYIKLCSGTTPRFFQYQVT RNAi Knockdown (Antibody KATCFQPLRQRVDTMENSAIKILRERVISRKSDLTRAFQLQDHRKSGKLS validated) VSQWAFCMENILGLNLPWRSLSSNLVNIDQNGNVEYMSSFQNIRIEKPVQ EAHSTLVETLYRYRSDLEIIFNAIDTDHSGLISVEEFRAMWKLFSSHYNVHI DDSQVNKLANIMDLNKDGSIDFNEFLKAFYVVHRYEDLMKPDVTNLG

Host: Mouse

Reactivity: Human enlarge

Isotype: IgG1 Kappa

Quality Control Antibody Reactive Against Recombinant Protein. Testing:

Western Blot detection against Immunogen (97.57 KDa) .

Storage Buffer: In 1x PBS, pH 7.4

Storage Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. Instruction:

MSDS: Download

Datasheet: Download

Applications

Page 1 of 3 2016/5/21 Western Blot (Transfected lysate)

Western Blot analysis of PPEF1 expression in transfected 293T cell line by PPEF1 monoclonal antibody (M01), clone 1F6-1A5.

Lane 1: PPEF1 transfected lysate(75.8 KDa). Lane 2: Non-transfected lysate. Protocol Download

Western Blot (Recombinant protein) Protocol Download

ELISA

RNAi Knockdown (Antibody validated)

Western blot analysis of PPEF1 over-expressed 293 cell line, cotransfected with PPEF1 Validated Chimera RNAi ( Cat # H00005475-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PPEF1 monoclonal antibody (M01), clone 1F6-1A5 (Cat # H00005475-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Protocol Download

Gene Information

Entrez GeneID: 5475

GeneBank BC036026 Accession#:

Protein AAH36026 Accession#:

Gene Name: PPEF1

Gene Alias: PP7,PPEF,PPP7C

Gene protein phosphatase, EF-hand calcium binding domain 1 Description:

Page 2 of 3 2016/5/21 Omim ID: 300109

Gene Ontology: Hyperlink

Gene Summary: This gene encodes a member of the serine/threonine protein phosphatase with EF-hand motif family. The protein contains a protein phosphatase catalytic domain, and at least two EF-hand calcium- binding motifs in its C terminus. Although its substrate(s) is unknown, the encoded protein has been suggested to play a role in specific sensory neuron function and/or development. This gene shares high sequence similarity with the Drosophila retinal degeneration C (rdgC) gene. Several alternatively spliced transcript variants, each encoding a distinct isoform, have been described. [provided by RefSeq

Other OTTHUMP00000023006,protein phosphatase with EF hand calcium- Designations: binding domain 1,protein phosphatase, EF hand calcium-binding domain 1,protein phosphatase, serine/threonine type, with EF- hands,serine/threonine protein phosphatase 7

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