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Table S1. 103 Ferroptosis-Related Genes Retrieved from the Genecards

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Table S1. 103 ferroptosis-related genes retrieved from the GeneCards. Gene Symbol Description Category GPX4 Glutathione Peroxidase 4 Protein Coding AIFM2 Apoptosis Inducing Factor Mitochondria Associated 2 Protein Coding TP53 Tumor Protein P53 Protein Coding ACSL4 Acyl-CoA Synthetase Long Chain Family Member 4 Protein Coding SLC7A11 Solute Carrier Family 7 Member 11 Protein Coding VDAC2 Voltage Dependent Anion Channel 2 Protein Coding VDAC3 Voltage Dependent Anion Channel 3 Protein Coding ATG5 Autophagy Related 5 Protein Coding ATG7 Autophagy Related 7 Protein Coding NCOA4 Nuclear Receptor Coactivator 4 Protein Coding HMOX1 Heme Oxygenase 1 Protein Coding SLC3A2 Solute Carrier Family 3 Member 2 Protein Coding ALOX15 Arachidonate 15-Lipoxygenase Protein Coding BECN1 Beclin 1 Protein Coding PRKAA1 Protein Kinase AMP-Activated Catalytic Subunit Alpha 1 Protein Coding SAT1 Spermidine/Spermine N1-Acetyltransferase 1 Protein Coding NF2 Neurofibromin 2 Protein Coding YAP1 Yes1 Associated Transcriptional Regulator Protein Coding FTH1 Ferritin Heavy Chain 1 Protein Coding TF Transferrin Protein Coding TFRC Transferrin Receptor Protein Coding FTL Ferritin Light Chain Protein Coding CYBB Cytochrome B-245 Beta Chain Protein Coding GSS Glutathione Synthetase Protein Coding CP Ceruloplasmin Protein Coding PRNP Prion Protein Protein Coding SLC11A2 Solute Carrier Family 11 Member 2 Protein Coding SLC40A1 Solute Carrier Family 40 Member 1 Protein Coding STEAP3 STEAP3 Metalloreductase Protein Coding ACSL1 Acyl-CoA Synthetase Long Chain Family Member 1 Protein Coding GCLC Glutamate-Cysteine Ligase Catalytic Subunit Protein Coding MAP1LC3A Microtubule Associated Protein 1 Light Chain 3 Alpha Protein Coding MAP1LC3B Microtubule Associated Protein 1 Light Chain 3 Beta Protein Coding SLC39A14 Solute Carrier Family 39 Member 14 Protein Coding SLC39A8 Solute Carrier Family 39 Member 8 Protein Coding ACSL5 Acyl-CoA Synthetase Long Chain Family Member 5 Protein Coding GCLM Glutamate-Cysteine Ligase Modifier Subunit Protein Coding PCBP1 Poly(RC) Binding Protein 1 Protein Coding PCBP2 Poly(RC) Binding Protein 2 Protein Coding ACSL3 Acyl-CoA Synthetase Long Chain Family Member 3 Protein Coding ACSL6 Acyl-CoA Synthetase Long Chain Family Member 6 Protein Coding SAT2 Spermidine/Spermine N1-Acetyltransferase Family Member 2 Protein Coding FTMT Ferritin Mitochondrial Protein Coding LPCAT3 Lysophosphatidylcholine Acyltransferase 3 Protein Coding MAP1LC3C Microtubule Associated Protein 1 Light Chain 3 Gamma Protein Coding MAP1LC3B2 Microtubule Associated Protein 1 Light Chain 3 Beta 2 Protein Coding BAP1 BRCA1 Associated Protein 1 Protein Coding PRDX6 Peroxiredoxin 6 Protein Coding SESN2 Sestrin 2 Protein Coding ARNTL Aryl Hydrocarbon Receptor Nuclear Translocator Like Protein Coding CISD1 CDGSH Iron Sulfur Domain 1 Protein Coding PROM2 Prominin 2 Protein Coding NEDD4 NEDD4 E3 Ubiquitin Protein Ligase Protein Coding CA9 Carbonic Anhydrase 9 Protein Coding ELAVL1 ELAV Like RNA Binding Protein 1 Protein Coding NFE2L2 Nuclear Factor, Erythroid 2 Like 2 Protein Coding ITGA6 Integrin Subunit Alpha 6 Protein Coding PRKAA2 Protein Kinase AMP-Activated Catalytic Subunit Alpha 2 Protein Coding FANCD2 FA Complementation Group D2 Protein Coding LAMP2 Lysosomal Associated Membrane Protein 2 Protein Coding ALOX12 Arachidonate 12-Lipoxygenase, 12S Type Protein Coding CD44 CD44 Molecule (Indian Blood Group) Protein Coding MAPK1 Mitogen-Activated Protein Kinase 1 Protein Coding MYC MYC Proto-Oncogene, BHLH Transcription Factor Protein Coding EGLN1 Egl-9 Family Hypoxia Inducible Factor 1 Protein Coding GOT1 Glutamic-Oxaloacetic Transaminase 1 Protein Coding MAP3K5 Mitogen-Activated Protein Kinase Kinase Kinase 5 Protein Coding ATF4 Activating Transcription Factor 4 Protein Coding FH Fumarate Hydratase Protein Coding HELLS Helicase, Lymphoid Specific Protein Coding SOCS1 Suppressor Of Cytokine Signaling 1 Protein Coding OTUB1 OTU Deubiquitinase, Ubiquitin Aldehyde Binding 1 Protein Coding CARS1 Cysteinyl-TRNA Synthetase 1 Protein Coding MIR9-1 MicroRNA 9-1 RNA Gene MIR137 MicroRNA 137 RNA Gene HMGB1 High Mobility Group Box 1 Protein Coding LINC00336 Long Intergenic Non-Protein Coding RNA 336 RNA Gene NFS1 NFS1 Cysteine Desulfurase Protein Coding PEBP1 Phosphatidylethanolamine Binding Protein 1 Protein Coding RB1 RB Transcriptional Corepressor 1 Protein Coding G3BP1 G3BP Stress Granule Assembly Factor 1 Protein Coding LINC00472 Long Intergenic Non-Protein Coding RNA 472 RNA Gene EPAS1 Endothelial PAS Domain Protein 1 Protein Coding HILPDA Hypoxia Inducible Lipid Droplet Associated Protein Coding PRC1 Protein Regulator Of Cytokinesis 1 Protein Coding NGB Neuroglobin Protein Coding MDM2 MDM2 Proto-Oncogene Protein Coding TIGAR TP53 Induced Glycolysis Regulatory Phosphatase Protein Coding VDAC1 Voltage Dependent Anion Channel 1 Protein Coding HSPB1 Heat Shock Protein Family B (Small) Member 1 Protein Coding HSPA5 Heat Shock Protein Family A (Hsp70) Member 5 Protein Coding CDKN2A Cyclin Dependent Kinase Inhibitor 2A Protein Coding CASP8 Caspase 8 Protein Coding CFTR CF Transmembrane Conductance Regulator Protein Coding AURKA Aurora Kinase A Protein Coding MIF Macrophage Migration Inhibitory Factor Protein Coding RIPK1 Receptor Interacting Serine/Threonine Kinase 1 Protein Coding MUC1 Mucin 1, Cell Surface Associated Protein Coding ALOX15B Arachidonate 15-Lipoxygenase Type B Protein Coding ANO6 Anoctamin 6 Protein Coding GUCY1A1 Guanylate Cyclase 1 Soluble Subunit Alpha 1 Protein Coding MT1G Metallothionein 1G Protein Coding MIR7-1 MicroRNA 7-1 RNA Gene GeneCards: https://www.genecards.org/ Table S2. The status of chromosome copy number aberrations of TCGA-UVM cases. Patient ID 3 CN (ABSOLUTE) 8q CN (ABSOLUTE) 6p CN (ABSOLUTE) TCGA-V3-A9ZY 2 2 3 TCGA-V4-A9EC 2 2 2 TCGA-V4-A9EH 2 2 2 TCGA-V4-A9EY 2 2 3 (less than half) TCGA-V4-A9F7 2 2 3 TCGA-VD-A8KE 2 2 3 TCGA-VD-A8KO 2 2 3 TCGA-VD-AA8M 2 2 2 TCGA-VD-AA8Q 2 2 2 TCGA-VD-AA8R 2 2 2 TCGA-WC-A87T 2 2 3 (less than half) TCGA-WC-A87U 2 2 3 TCGA-WC-A880 2 2 3 TCGA-WC-A884 2 2 3 TCGA-YZ-A983 2 2 3 TCGA-V4-A9E5 2 3 4 TCGA-V4-A9E9 2 3 3 TCGA-V4-A9EA 2 3 3 TCGA-V4-A9EJ 2 3 4 TCGA-V4-A9EK 2 3 4 TCGA-V4-A9EM 2 4 3 TCGA-V4-A9ET 2 4 4 TCGA-V4-A9EW 2 2 4 TCGA-V4-A9EZ 2 3 3 TCGA-V4-A9F2 2 3 3 TCGA-V4-A9F4 2 3 4 TCGA-VD-A8K7 2 2 4 TCGA-VD-A8K9 2 3 5 TCGA-VD-A8KA 2 3 4 TCGA-VD-A8KB 2 4 4 TCGA-VD-A8KG 2 4 4 TCGA-VD-A8KJ 2 4 3 and 4 (roughly half) TCGA-VD-AA8S 2 3 4 TCGA-WC-A87W 2 2 3 TCGA-WC-A881 2 5 3 TCGA-WC-A885 2 3 4 TCGA-WC-AA9E 2 3 5 TCGA-YZ-A982 3 4 5 TCGA-V4-A9E8 1 3 3 TCGA-V4-A9ED 1 2 2 TCGA-V4-A9EF 1 4 2 TCGA-V4-A9EO 2, LOH 5 4 TCGA-V4-A9EQ 1 3 2 TCGA-V4-A9ES 1 2 2 TCGA-V4-A9F0 1 3 2 TCGA-V4-A9F8 1 4 2 TCGA-VD-A8KD 1 3 2 TCGA-VD-A8KF 1 3 2 TCGA-VD-A8KH 1 3 2 TCGA-VD-A8KK 1 3 2 TCGA-VD-A8KL 2, LOH 5 4 TCGA-VD-AA8O 2, LOH 4 3 TCGA-VD-AA8P 1 3 2 TCGA-VD-AA8T 1 3 2 TCGA-WC-A882 1 3 2 TCGA-WC-A888 2, Subclonal LOH 3 2 TCGA-WC-AA9A 1 3 2 TCGA-YZ-A980 1 3 2 TCGA-YZ-A984 2, LOH 4 3 TCGA-YZ-A985 1 2 2 TCGA-RZ-AB0B 1 7 2 TCGA-V3-A9ZX 1 7 3 TCGA-V4-A9E7 1 5 2 TCGA-V4-A9EE 1 5 2 TCGA-V4-A9EI 1 8 3 TCGA-V4-A9EL 1 7 2 TCGA-V4-A9EU 1 6 2 TCGA-V4-A9EV 1 5 2 TCGA-V4-A9EX 1 6 3 TCGA-V4-A9F1 1 5 2 TCGA-V4-A9F3 1 5 3 TCGA-V4-A9F5 1 4 2 TCGA-VD-A8K8 1 5 2 TCGA-VD-A8KI 1 5 3 TCGA-VD-A8KM 1 3 3 TCGA-VD-A8KN 1 4 2 TCGA-VD-AA8N 1 4 2 TCGA-WC-A87Y 1 4 2 TCGA-WC-A883 1 6 2 TCGA-WC-A88A 1 4 3 Table S3. Autophagy-related genes. No. Gene Symbol No. Gene Symbol No. Gene Symbol No. Gene Symbol No. Gene Symbol 1 ABL1 101 CSNK2A2 201 HTT 301 OSBPL7 401 SOGA3 2 ABL2 102 CTSA 202 IFI16 302 P4HB 402 SPHK1 3 ACER2 103 CTSB 203 IFNG 303 PAFAH1B2 403 SPNS1 4 ADRA1A 104 CTSD 204 IFT20 304 PARK2 404 SPTLC1 5 ADRB2 105 CTTN 205 IFT88 305 PARK7 405 SPTLC2 6 AKT1 106 CX3CL1 206 IKBKB 306 PARP1 406 SQSTM1 7 AMBRA1 107 CXCR4 207 IKBKE 307 PEA15 407 SREBF1 8 APOL1 108 DAP 208 IKBKG 308 PELP1 408 SREBF2 9 ARNT 109 DAPK1 209 IL10 309 PEX14 409 ST13 10 ARSA 110 DAPK2 210 IL10RA 310 PEX3 410 STAT3 11 ARSB 111 DAPK3 211 IL24 311 PHF23 411 STK11 12 ATF4 112 DAPL1 212 IL4 312 PIK3C3 412 STUB1 13 ATF6 113 DCN 213 IRGM 313 PIK3CA 413 STX12 14 ATG10 114 DDIT3 214 ITGA3 314 PIK3CB 414 SUPT5H 15 ATG101 115 DHRSX 215 ITGA6 315 PIK3R2 415 SVIP 16 ATG12 116 DIRAS3 216 ITGB1 316 PIK3R4 416 SYNPO2 17 ATG13 117 DLC1 217 ITGB4 317 PIKFYVE 417 TAB2 18 ATG14 118 DNAJB1 218 ITPR1 318 PIM2 418 TAB3 19 ATG16L1 119 DNAJB9 219 KAT5 319 PINK1 419 TBC1D12 20 ATG16L2 120 DNM1L 220 KAT8 320 PIP4K2A 420 TBC1D14 21 ATG2A 121 DRAM1 221 KDM4A 321 PIP4K2B 421 TBC1D25 22 ATG2B 122 DRAM2 222 KDR 322 PIP4K2C 422 TBK1 23 ATG3 123 EDEM1 223 KEAP1 323 PLEKHF1 423 TEX264 24 ATG4A 124 EEF1A1 224 KIAA0226 324 PLK2 424 TFEB 25 ATG4B 125 EEF1A2 225 KIAA1324 325 PLK3 425 TICAM1 26 ATG4C 126 EEF2 226 KIF25 326 POLDIP2 426 TLK2 27 ATG4D 127 EEF2K 227 KIF5B 327 PPP1R15A 427 TM9SF1 28 ATG5 128 EGFR 228 KLHL22 328 PRKAA1 428 TMEM150A 29 ATG7 129 EIF2AK2 229 KLHL24 329 PRKAA2 429 TMEM150B 30 ATG9A 130 EIF2AK3 230 KLHL3 330 PRKAB1 430 TMEM150C 31 ATG9B 131 EIF2AK4 231 LACRT 331 PRKAB2 431 TMEM59 32 ATIC 132 EIF2S1 232 LAMP1 332 PRKACA 432 TMEM74 33 ATM 133 EIF4EBP1 233 LAMP2 333 PRKAG1 433 TNFSF10 34 ATP13A2 134 EIF4G1 234 LAMP3 334 PRKAG2 434 TOMM7 35 ATP6V0A1 135 EIF4G2 235 LAMTOR1 335 PRKAG3 435 TP53 36 ATP6V0A2 136 EP300 236 LAMTOR2 336 PRKAR1A 436 TP53INP1 37 ATP6V0B 137 EPM2A 237 LAMTOR3 337 PRKCD 437 TP53INP2 38 ATP6V0C 138 ERBB2 238 LAMTOR4 338 PRKCQ 438 TP63 39 ATP6V0D1 139 ERCC4 239 LAMTOR5 339 PRKD1 439 TP73 40 ATP6V0D2 140 ERN1 240 LARP1 340 PSAP 440 TPCN1 41 ATP6V0E1 141 ERO1L 241 LEP 341 PTEN 441 TPCN2 42 ATP6V0E2 142 EXOC1
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  • Usbiological Datasheet

    Usbiological Datasheet

    ABI2 (ABI-2, ABI2B, AblBP3, AIP-1, argBPIA, SSH3BP2, ARGBPIA, Abl interactor 2) Catalog number 144114 Supplier United States Biological ABI2 (ABL Interactor 2), is a protein that in humans is encoded by the ABI2 gene. By analysis of a YAC and a BAC, Machado et al. (2000) mapped the ABI2 gene to 2q31-q33. ABI2 possesses a basic N terminus with homology to a homeodomain protein; a central serine-rich region; 3 PEST sequences, which are implicated in susceptibility to protein degradation; several proline-rich stretches; and an acidic C terminus with multiple phosphorylation sites and an SH3 domain. Dai and Pendergast (1995) suggested that the ABI proteins may function to coordinate the cytoplasmic and nuclear functions of the ABL1 tyrosine kinase. UniProt Number Q9NYB9 Gene ID ABI2 Applications Suitable for use in Western Blot. Recommended Dilution Optimal dilutions to be determined by the researcher. Storage and Handling Store at -20˚C for one year. After reconstitution, store at 4˚C for one month. Can also be aliquoted and stored frozen at -20˚C for long term. Avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Immunogen A synthetic peptide corresponding to a sequence at the N-terminal of human ABI2, identical to the related mouse and rat sequences. Formulation Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
  • DYSREGULATION of LONG-CHAIN ACYL-Coa SYNTHETASES in CANCER and THEIR TARGETING STRATEGIES in ANTICANCER THERAPY Md Amir Hossain1, Jun Ma2, Yong Yang1 1

    DYSREGULATION of LONG-CHAIN ACYL-Coa SYNTHETASES in CANCER and THEIR TARGETING STRATEGIES in ANTICANCER THERAPY Md Amir Hossain1, Jun Ma2, Yong Yang1 1

    Hossain et al RJLBPCS 2020 www.rjlbpcs.com Life Science Informatics Publications Original Review Article DOI: 10.26479/2020.0603.06 DYSREGULATION OF LONG-CHAIN ACYL-CoA SYNTHETASES IN CANCER AND THEIR TARGETING STRATEGIES IN ANTICANCER THERAPY Md Amir Hossain1, Jun Ma2, Yong Yang1 1. Academic Institute of Pharmaceutical Science, China Pharmaceutical University, Nanjing, China. 2. Ningxia Baoshihua Hospital, Ningxia, China. ABSTRACT: Cancer is a leading cause of death worldwide, and the number of cases globally continues to increase. Cancer is caused by certain changes in genes that are involved in controlling cell functions and cell division. Notably, metabolic dysregulation is one the hallmarks of cancer, and increases in fatty acid metabolism have been demonstrated to promote the growth and survival of a variety of cancers. In human, fatty acids either can be breakdown into acetyl-CoA through catabolic metabolism and aid in ATP generation or in the anabolic metabolism they can incorporate into triacylglycerol and phospholipid. Importantly, both of these pathways need activation of fatty acids, and the key players in this activation of fatty acids are the long-chain acyl-CoA synthetases (ACSLs) that are commonly dysregulated in cancer and associated with oncogenesis and survival. Therefore, it provides a rationale to target ACSLs in cancer. This review summarizes the current understanding of long-chain acyl-CoA synthetases in cancer and their targeting opportunities. KEYWORDS: Acyl-CoA synthetases, cancer, fatty acid, lipid metabolism. Article History: Received: April 15, 2020; Revised: May 10, 2020; Accepted: May 30, 2020. Corresponding Author: Prof. Yong Yang* Academic Institute of Pharmaceutical Science, China Pharmaceutical University, Nanjing, China.
  • Supplementary Materials

    Supplementary Materials

    Supplementary materials Supplementary Table S1: MGNC compound library Ingredien Molecule Caco- Mol ID MW AlogP OB (%) BBB DL FASA- HL t Name Name 2 shengdi MOL012254 campesterol 400.8 7.63 37.58 1.34 0.98 0.7 0.21 20.2 shengdi MOL000519 coniferin 314.4 3.16 31.11 0.42 -0.2 0.3 0.27 74.6 beta- shengdi MOL000359 414.8 8.08 36.91 1.32 0.99 0.8 0.23 20.2 sitosterol pachymic shengdi MOL000289 528.9 6.54 33.63 0.1 -0.6 0.8 0 9.27 acid Poricoic acid shengdi MOL000291 484.7 5.64 30.52 -0.08 -0.9 0.8 0 8.67 B Chrysanthem shengdi MOL004492 585 8.24 38.72 0.51 -1 0.6 0.3 17.5 axanthin 20- shengdi MOL011455 Hexadecano 418.6 1.91 32.7 -0.24 -0.4 0.7 0.29 104 ylingenol huanglian MOL001454 berberine 336.4 3.45 36.86 1.24 0.57 0.8 0.19 6.57 huanglian MOL013352 Obacunone 454.6 2.68 43.29 0.01 -0.4 0.8 0.31 -13 huanglian MOL002894 berberrubine 322.4 3.2 35.74 1.07 0.17 0.7 0.24 6.46 huanglian MOL002897 epiberberine 336.4 3.45 43.09 1.17 0.4 0.8 0.19 6.1 huanglian MOL002903 (R)-Canadine 339.4 3.4 55.37 1.04 0.57 0.8 0.2 6.41 huanglian MOL002904 Berlambine 351.4 2.49 36.68 0.97 0.17 0.8 0.28 7.33 Corchorosid huanglian MOL002907 404.6 1.34 105 -0.91 -1.3 0.8 0.29 6.68 e A_qt Magnogrand huanglian MOL000622 266.4 1.18 63.71 0.02 -0.2 0.2 0.3 3.17 iolide huanglian MOL000762 Palmidin A 510.5 4.52 35.36 -0.38 -1.5 0.7 0.39 33.2 huanglian MOL000785 palmatine 352.4 3.65 64.6 1.33 0.37 0.7 0.13 2.25 huanglian MOL000098 quercetin 302.3 1.5 46.43 0.05 -0.8 0.3 0.38 14.4 huanglian MOL001458 coptisine 320.3 3.25 30.67 1.21 0.32 0.9 0.26 9.33 huanglian MOL002668 Worenine