DOCSLIB.ORG

Basophil/Mast Cell Progenitors in the Spleen Marrow and by Increasing

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Basophil/Mast Cell Progenitors in the Spleen Marrow and by Increasing IL-3 Induces Basophil Expansion In Vivo by Directing Granulocyte-Monocyte Progenitors to Differentiate into Basophil Lineage-Restricted Progenitors in the Bone This information is current as Marrow and by Increasing the Number of of September 26, 2021. Basophil/Mast Cell Progenitors in the Spleen Keitaro Ohmori, Yuchun Luo, Yi Jia, Jun Nishida, Zhengqi Wang, Kevin D. Bunting, Demin Wang and Hua Huang Downloaded from J Immunol 2009; 182:2835-2841; ; doi: 10.4049/jimmunol.0802870 http://www.jimmunol.org/content/182/5/2835 http://www.jimmunol.org/ References This article cites 41 articles, 22 of which you can access for free at: http://www.jimmunol.org/content/182/5/2835.full#ref-list-1 Why The JI? Submit online. • Rapid Reviews! 30 days* from submission to initial decision by guest on September 26, 2021 • No Triage! Every submission reviewed by practicing scientists • Fast Publication! 4 weeks from acceptance to publication *average Subscription Information about subscribing to The Journal of Immunology is online at: http://jimmunol.org/subscription Permissions Submit copyright permission requests at: http://www.aai.org/About/Publications/JI/copyright.html Email Alerts Receive free email-alerts when new articles cite this article. Sign up at: http://jimmunol.org/alerts The Journal of Immunology is published twice each month by The American Association of Immunologists, Inc., 1451 Rockville Pike, Suite 650, Rockville, MD 20852 Copyright © 2009 by The American Association of Immunologists, Inc. All rights reserved. Print ISSN: 0022-1767 Online ISSN: 1550-6606. The Journal of Immunology IL-3 Induces Basophil Expansion In Vivo by Directing Granulocyte-Monocyte Progenitors to Differentiate into Basophil Lineage-Restricted Progenitors in the Bone Marrow and by Increasing the Number of Basophil/Mast Cell Progenitors in the Spleen1 Keitaro Ohmori,2* Yuchun Luo,* Yi Jia,* Jun Nishida,* Zhengqi Wang,† Kevin D. Bunting,† Demin Wang,‡§ and Hua Huang3*¶ Recent work has established important roles for basophils in regulating immune responses. To exert their biological functions, Downloaded from basophils need to be expanded to critical numbers. However, the mechanisms underlying basophil expansion remain unclear. In this study, we established that IL-3 played an important role in the rapid and specific expansion of basophils. We found that the IL-3 complex (IL-3 plus anti-IL-3 Ab) greatly facilitated the differentiation of GMPs into basophil lineage-restricted progenitors (BaPs) but not into eosinophil lineage-restricted progenitors or mast cells in the bone marrow. We also found that the IL-3 complex treatment resulted in ϳ4-fold increase in the number of basophil/mast cell progenitors (BMCPs) in the spleen. IL-3-driven basophil expansion depended on STAT5 signaling. We showed that GMPs but not common myeloid progenitors expressed low levels of IL-3 receptor. IL-3 receptor http://www.jimmunol.org/ expression was dramatically up-regulated in BaPs but not eosinophil lineage-restricted progenitors. Approximately 38% of BMCPs expressed the IL-3R␣-chain. The up-regulated IL-3 receptor expression was not affected by IL-3 or STAT5. Our findings demonstrate that IL-3 induced specific expansion of basophils by directing GMPs to differentiate into BaPs in the bone marrow and by increasing the number of BMCPs in the spleen. The Journal of Immunology, 2009, 182: 2835–2841. ecent work has established important roles for basophils dramatically in number after infection with intestinal nematode, in initiating (1–3) and augmenting Th2-type immune Nippostrongylus brasiliensis (11). They also increase in number responses to allergen challenges and parasitic infection after protease allergen challenge (2). ϩ R by guest on September 26, 2021 (4–6), mediating anaphylaxis (7), and maintaining B cell memory IL-3, produced primarily by CD4 T cells (12–14), has been responses (8). However, due to the difficulty of obtaining a suffi- shown to be pivotal in expanding basophils. In vitro, IL-3 has been cient numbers of basophils, basophil biology has not yet been stud- demonstrated to induce basophil differentiation and to enhance ied extensively. acute IL-4 production in mouse basophils (15–17). Galli and col- Basophils are a minor cell population, constituting Ͻ1% of pe- leagues (18) reported that mice lacking IL-3 failed to show in- ripheral blood and bone marrow cells. Basophils are normally gen- creased numbers of basophils and failed to expel nematode Strong- erated from GMPs, which also give rise to neutrophils, monocytes, lyoides. Nonetheless, mechanisms underlying basophil expansion eosinophils, and mast cells. These cells share many common char- remain obscure. Mature basophils have a short half-life and re- acteristics with mast cells, such as expression of a high affinity spond to IL-3 stimulation with a limited proliferation capacity (13, immunoglobin (IgE) receptor (Fc␧RI), and contain many of the 19). Thus, it is likely that IL-3 might induce basophil expansion by granules that can be found in mast cells (9, 10). Basophils expand enhancing basophil lineage commitment. In this study, we report that administrating the IL-3 complex (IL-3 plus anti-IL-3 Ab) in vivo greatly facilitated the differenti- *Division of Allergy and Immunology, Departments of Medicine, National Jewish 4 Health, Denver, CO 80206; †Departments of Medicine, Case Western Reserve Uni- ation of GMPs into basophil lineage-restricted progenitors (BaPs) versity, Cleveland, OH 44106; ‡Blood Research Institute, Blood Center of Wisconsin, specifically in a STAT5 signaling-dependent manner and increased § Milwaukee, WI 53226; Department of Microbiology and Molecular Genetics, Med- the number of basophil/mast cell progenitors (BMCPs) in the ical College of Wisconsin, Milwaukee, WI 53226; and ¶Integrated Department of Immunology, University of Colorado Health Sciences Center, Denver, CO 80206 spleen. We showed that GMPs, but not common myeloid progen- Received for publication August 29, 2008. Accepted for publication December itors (CMPs), expressed low levels of IL-3 receptor. The IL-3 re- 22, 2008. ceptor expression was dramatically up-regulated in BaPs, but not The costs of publication of this article were defrayed in part by the payment of page eosinophil lineage-restricted progenitors (EoPs). We showed that charges. This article must therefore be hereby marked advertisement in accordance ϳ38% of BMCPs expressed the IL-3 receptor. The IL-3 receptor with 18 U.S.C. Section 1734 solely to indicate this fact. expression patterns might explain why IL-3 specifically expanded 1 This work is supported by grants from the National Institutes of Health R01 AI48568 and R01 AI068083 (H.H.), R01 HL073284 and R01 AI079087 (D.W.), Scholar Award basophils in vivo. from the Leukemia & Lymphoma Society (D.W.), and R01DK059380 (K.B.). 2 Current address: Tokyo University of Agriculture and Technology, 3-5-8 Saiwai- cho, Fuchu, Tokyo, Japan. 4 Abbreviations used in this paper: BaP, basophil lineage-restricted progenitor; 3 Address correspondence and reprint requests to Dr. Hua Huang, Departments of BMCP, basophil/mast cell progenitor; CMP, common myeloid progenitor; EoP, eo- Medicine and Immunology, National Jewish Health and University of Colorado sinophil lineage-restricted progenitor. Health Sciences Center, 1400 Jackson Street, Denver, CO 80206. E-mail address: [email protected] Copyright © 2009 by The American Association of Immunologists, Inc. 0022-1767/09/$2.00 www.jimmunol.org/cgi/doi/10.4049/jimmunol.0802870 2836 BASOPHIL DEVELOPMENT AND EXPANSION Downloaded from http://www.jimmunol.org/ by guest on September 26, 2021 FIGURE 1. IL-3 dramatically and specifically increases the percentage and number of basophils. A, C57BL/6J mice were or were not injected with IL-3 alone, anti-IL-3 Ab alone or IL-3 mixed with anti-IL-3 Ab (IL-3 complex) i.v. via a tail vein. Three days after injection, the bone marrow cells were isolated and stained with PE-labeled anti-Fc␧RI␣ and FITC-labeled anti-c-kit Abs. Basophils were defined as Fc␧RI␣ϩc-kitϪ. Data are representative of five independent experiments. B, A time course analysis of basophil numbers in the bone marrow. Two to five mice were used for each time point. Error bars show the SD from two to five mice. C, C57BL/6J mice were or were not injected with the IL-3 complex. Three days after injection, the bone marrow, peripheral blood, spleen, and peritoneal cavity cells were isolated and stained with PE-labeled anti-Fc␧RI␣ and FITC-labeled anti-c-kit Abs for basophil or mast detection or with PE-labeled anti-Siglec-F Ab for eosinophils detection. Mast cells were defined as Fc␧RI␣ϩc-kitϩ cells. Eosinophils were marked as Siglec-Fϩ cells. D, C57BL/6J mice were or were not injected with the IL-3 complex. The total number of basophil, eosinophil, and mast cell were shown. Error bars show the SD from two to five mice. p values were analyzed using Student’s t test. Data are representative of three independent experiments. Materials and Methods with FITC-labeled anti-c-kit (2B8), biotin-labeled anti-Fc␧RI␣ (MAR-1), and Mice PE-labeled Siglec-F (E50–2440) Abs, followed by streptavidin-allophyco- cyanin. Basophils were further characterized with PE-labeled anti-Thy1.2 C57BL/6J and B6.SJL-Ptprca Pepcb/BoyJ (CD45.1) mice were purchased (30-H12) or PE-labeled anti-CD49b (DX5) Abs. Basophils were defined as from The Jackson Laboratory. A pair of STAT5ϩ/Ϫ breeder mice (20) was Fc␧RI␣ϩc-kitϪ, while mast cells were defined as Fc␧RI␣ϩc-kitϩ cells. Eo- provided by Dr. Lothar Hennighausen of the National Institutes of Health sinophils were marked as Siglec-Fϩ or Siglec-Fϩ and CCR3ϩ cells (22, (Bethesda, Maryland). These mice have been bred and maintained under 23). For detecting donor-derived basophils, Pacific Blue-labeled anti- pathogen-free conditions at the animal facility of National Jewish Health.
Load more

Recommended publications
  • White Blood Cells and Severe COVID-19: a Mendelian Randomization Study
    Journal of Personalized Medicine Article White Blood Cells and Severe COVID-19: A Mendelian Randomization Study Yitang Sun 1 , Jingqi Zhou 1,2 and Kaixiong Ye 1,3,* 1 Department of Genetics, Franklin College of Arts and Sciences, University of Georgia, Athens, GA 30602, USA; [email protected] (Y.S.); [email protected] (J.Z.) 2 School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China 3 Institute of Bioinformatics, University of Georgia, Athens, GA 30602, USA * Correspondence: [email protected]; Tel.: +1-706-542-5898; Fax: +1-706-542-3910 Abstract: Increasing evidence shows that white blood cells are associated with the risk of coronavirus disease 2019 (COVID-19), but the direction and causality of this association are not clear. To evaluate the causal associations between various white blood cell traits and the COVID-19 susceptibility and severity, we conducted two-sample bidirectional Mendelian Randomization (MR) analyses with summary statistics from the largest and most recent genome-wide association studies. Our MR results indicated causal protective effects of higher basophil count, basophil percentage of white blood cells, and myeloid white blood cell count on severe COVID-19, with odds ratios (OR) per standard deviation increment of 0.75 (95% CI: 0.60–0.95), 0.70 (95% CI: 0.54–0.92), and 0.85 (95% CI: 0.73–0.98), respectively. Neither COVID-19 severity nor susceptibility was associated with white blood cell traits in our reverse MR results. Genetically predicted high basophil count, basophil percentage of white blood cells, and myeloid white blood cell count are associated with a lower risk of developing severe COVID-19.
    [Show full text]
  • Basophils Trigger Emphysema Development in a Murine Model of COPD Through IL-4–Mediated Generation of MMP-12–Producing Macrophages
    Basophils trigger emphysema development in a murine model of COPD through IL-4–mediated generation of MMP-12–producing macrophages Sho Shibataa,b, Kensuke Miyakea, Tomoya Tateishib, Soichiro Yoshikawaa, Yoshinori Yamanishia, Yasunari Miyazakib, Naohiko Inaseb, and Hajime Karasuyamaa,1 aDepartment of Immune Regulation, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), Tokyo 113-8510, Japan; and bDepartment of Respiratory Medicine, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), Tokyo 113-8510, Japan Edited by Kazuhiko Yamamoto, the University of Tokyo; Max Planck–The University of Tokyo Center for Integrative Inflammology, the University of Tokyo, Tokyo, Japan, and accepted by Editorial Board Member Tadatsugu Taniguchi November 2, 2018 (received for review August 13, 2018) Chronic obstructive pulmonary disease (COPD) is a leading cause of COPD even after smoking has stopped (9). As an alternative morbidity and mortality worldwide. It has generally been consid- approach, a protease-induced model was established (10) soon ered a non-Th2-type lung disorder, characterized by progressive after the discovery of the association between α1-antitrypsin airflow limitation with inflammation and emphysema, but its deficiency and emphysema in humans (11), implying that an im- cellular and molecular mechanism remains ill defined, compared balance between proteases and antiproteases in the lung may re- with that of asthma characterized by reversible airway obstruc- sult in emphysema formation. Intratracheal or intranasal instillation tion. Here we show a previously unappreciated role for basophils of elastolytic enzymes, such as papain, human neutrophil elastase, at the initiation phase of emphysema formation in an elastase- and porcine pancreatic elastase, is utilized to trigger emphysema induced murine model of COPD in that basophils represent less formation (10, 12, 13).
    [Show full text]
  • Understanding the Immune System: How It Works
    Understanding the Immune System How It Works U.S. DEPARTMENT OF HEALTH AND HUMAN SERVICES NATIONAL INSTITUTES OF HEALTH National Institute of Allergy and Infectious Diseases National Cancer Institute Understanding the Immune System How It Works U.S. DEPARTMENT OF HEALTH AND HUMAN SERVICES NATIONAL INSTITUTES OF HEALTH National Institute of Allergy and Infectious Diseases National Cancer Institute NIH Publication No. 03-5423 September 2003 www.niaid.nih.gov www.nci.nih.gov Contents 1 Introduction 2 Self and Nonself 3 The Structure of the Immune System 7 Immune Cells and Their Products 19 Mounting an Immune Response 24 Immunity: Natural and Acquired 28 Disorders of the Immune System 34 Immunology and Transplants 36 Immunity and Cancer 39 The Immune System and the Nervous System 40 Frontiers in Immunology 45 Summary 47 Glossary Introduction he immune system is a network of Tcells, tissues*, and organs that work together to defend the body against attacks by “foreign” invaders. These are primarily microbes (germs)—tiny, infection-causing Bacteria: organisms such as bacteria, viruses, streptococci parasites, and fungi. Because the human body provides an ideal environment for many microbes, they try to break in. It is the immune system’s job to keep them out or, failing that, to seek out and destroy them. Virus: When the immune system hits the wrong herpes virus target or is crippled, however, it can unleash a torrent of diseases, including allergy, arthritis, or AIDS. The immune system is amazingly complex. It can recognize and remember millions of Parasite: different enemies, and it can produce schistosome secretions and cells to match up with and wipe out each one of them.
    [Show full text]
  • The Term Meaning White Blood Cells Is
    The Term Meaning White Blood Cells Is Micro Angelico still desalinate: venatic and unclimbed Elliot originate quite significatively but globes her proximocracoviennes and admeasuring catechetically. politely. Welsh Hazardableis attritional orand pollened, endows Sherwood voicelessly never as statant comb-outs Ira interpenetrates any output! Trophoblast cells are destined to give rise to many of the extraembryonic tissues. Genetic disorders and blood the cells is a permanent hair shaft, great care provider first line reported by lab technician may be included in circulation, the superficial veins. The part of the brain that controls coordinated movement. What Is A Medical Technologist? Inflammation of the liver. MPV is used along with platelet count to diagnose some diseases. After circulating in the bloodstream for about a day, monocytes enter body tissues to become macrophages, which can destroy some germs by surrounding and digesting them. The legacy of this great resource continues as the Merck Veterinary Manual in the US and Canada and the MSD Manual outside of North America. An abnormal increase in the volume of circulating blood. These small cells seem to sound an alarm when infectious agents invade your blood. Comparisons may be useful for a differential diagnosis. Finally, emotional or physical stress can also cause elevated white blood cell counts. The ability of the body to learn to fight specific infections after being exposed to the germs that cause them. However, you may also visit the Cookie Settings at any time to provide controlled consent, and you can withdraw your consent there. Severe pain that occurs suddenly and usually lasts a short while.
    [Show full text]
  • The Role of Eosinophils and Basophils in Allergic Diseases Considering Genetic Findings
    The role of eosinophils and basophils in allergic diseases considering genetic findings. Rachel Nadif, Farid Zerimech, Emmanuelle Bouzigon, Regis Matran To cite this version: Rachel Nadif, Farid Zerimech, Emmanuelle Bouzigon, Regis Matran. The role of eosinophils and ba- sophils in allergic diseases considering genetic findings.. Current Opinion in Allergy and Clinical Im- munology, Lippincott, Williams & Wilkins, 2013, 13 (5), pp.507-13. 10.1097/ACI.0b013e328364e9c0. inserm-00880260 HAL Id: inserm-00880260 https://www.hal.inserm.fr/inserm-00880260 Submitted on 3 Oct 2014 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. The role of eosinophils and basophils in allergic diseases considering genetic findings Rachel Nadifa,b, Farid Zerimechc,d, Emmanuelle Bouzigone,f, Regis Matranc,d Affiliations: aInserm, Centre for research in Epidemiology and Population Health (CESP), U1018, Respiratory and Environmental Epidemiology Team, F-94807, Villejuif, France bUniv Paris-Sud, UMRS 1018, F-94807, Villejuif, France cCHRU de Lille, F-59000, Lille, France dUniv Lille Nord de France, EA4483, F-59000, Lille, France eUniv Paris Diderot, Sorbonne Paris Cité, Institut Universitaire d’Hématologie, F-75007, Paris, France fInserm, UMR-946, F-75010, Paris, France Correspondence to Rachel Nadif, PhD, Inserm, Centre for research in Epidemiology and Population Health (CESP), U1018, Respiratory and Environmental Epidemiology Team, F-94807, Villejuif, France.
    [Show full text]
  • How Are White Blood Cells Classified?
    How are white blood cells classified? Copyright 2017 by the Rector and Visitors of the University of Virginia How are white blood cells classified? Types of White Blood Cells: Neutrophil Eosinophil Basophil Lymphocyte Monocyte . The types of white blood cells are shown above. The next page will describe lymphocytes in further detail. A healthy individual has all of these white blood cells types, but within specific ranges. Deviation from these ranges can indicate acute illness or a chronic disease. A mnemonic that is often used to remember the relative amount of each white blood cell that should be present is “Never Let Monkeys Eat Bananas.” Never Neutrophil Highest amounts Let Lymphocyte Monkeys Monocyte Eat Eosinophil Bananas Basophil Lowest amounts . In other words, neutrophils should always be present in higher amounts compared to the other cell types. This will be described further in “A first step in diagnosing LGL leukemia: The blood smear.” Copyright 2017 by the Rector and Visitors of the University of Virginia How are white blood cells classified? Introduction: White blood cells are blood cells that fight infection and disease. Lymphocytes are a type of white blood cell. They can identify antigens (substances foreign to the body) and cause an immune response. There are three types of lymphocytes: T-cell, NK-cell, and B-cell. In healthy adults, 10-15% of the lymphocytes are large granular lymphocytes (LGLs). To learn more about LGL cells, see “A first step in diagnosing LGL leukemia: The blood smear.” A person is diagnosed with LGL leukemia if there is a clonal (copied) population of T-cells or NK-cells present.
    [Show full text]
  • Granulocytes in Helminth Infection - Who Is Calling the Shots?
    Current Medicinal Chemistry, 2012, 19, 1567-1586 1567 Granulocytes in Helminth Infection - Who is Calling the Shots? B.L. Makepeace1, C. Martin2, J.D. Turner3 and S. Specht*,4 1Department of Infection Biology, Institute of Infection & Global Health, University of Liverpool, Liverpool L69 7ZJ, UK 2USM 7245 MCAM, CNRS MNHN, Muséum National d’Histoire Naturelle, 61 rue Buffon, 75231 Paris Cedex 05, France 3Department of Molecular and Biochemical Parasitology, Liverpool School of Tropical Medicine, Liverpool, L3 5QA, UK 4Institute for Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, 53105 Bonn, Germany Abstract: Helminths are parasitic organisms that can be broadly described as “worms” due to their elongated body plan, but which otherwise differ in shape, development, migratory routes and the predilection site of the adults and larvae. They are divided into three major groups: trematodes (flukes), which are leaf-shaped, hermaphroditic (except for blood flukes) flatworms with oral and ventral suckers; cestodes (tapeworms), which are segmented, hermaphroditic flatworms that inhabit the intestinal lumen; and nematodes (roundworms), which are dioecious, cylindrical parasites that inhabit intestinal and peripheral tissue sites. Helminths exhibit a sublime co-evolution with the host´s immune system that has enabled them to successfully colonize almost all multicellular species present in every geographical environment, including over two billion humans. In the face of this challenge, the host immune system has evolved to strike a delicate balance between attempts to neutralize the infectious assault versus limitation of damage to host tissues. Among the most important cell types during helminthic invasion are granulocytes: eosinophils, neutrophils and basophils. Depending on the specific context, these leukocytes may have pivotal roles in host protection, immunopathology, or facilitation of helminth establishment.
    [Show full text]
  • Basophil Activation Receptors Inhibit Mouse and Human Γ Fc
    Fcγ Receptors Inhibit Mouse and Human Basophil Activation Lydie Cassard, Friederike Jönsson, Ségolène Arnaud and Marc Daëron This information is current as of September 24, 2021. J Immunol published online 20 August 2012 http://www.jimmunol.org/content/early/2012/08/20/jimmun ol.1200968 Downloaded from Supplementary http://www.jimmunol.org/content/suppl/2012/08/20/jimmunol.120096 Material 8.DC1 Why The JI? Submit online. http://www.jimmunol.org/ • Rapid Reviews! 30 days* from submission to initial decision • No Triage! Every submission reviewed by practicing scientists • Fast Publication! 4 weeks from acceptance to publication *average by guest on September 24, 2021 Subscription Information about subscribing to The Journal of Immunology is online at: http://jimmunol.org/subscription Permissions Submit copyright permission requests at: http://www.aai.org/About/Publications/JI/copyright.html Email Alerts Receive free email-alerts when new articles cite this article. Sign up at: http://jimmunol.org/alerts The Journal of Immunology is published twice each month by The American Association of Immunologists, Inc., 1451 Rockville Pike, Suite 650, Rockville, MD 20852 Copyright © 2012 by The American Association of Immunologists, Inc. All rights reserved. Print ISSN: 0022-1767 Online ISSN: 1550-6606. Published August 20, 2012, doi:10.4049/jimmunol.1200968 The Journal of Immunology Fcg Receptors Inhibit Mouse and Human Basophil Activation Lydie Cassard,*,†,1 Friederike Jo¨nsson,*,†,1 Se´gole`ne Arnaud,*,† and Marc Dae¨ron*,† Besides high-affinity IgE receptors (Fc«RI), human basophils express activating (FcgRIIA) and inhibitory (FcgRIIB) low-affinity IgG receptors. IgG receptors (FcgR) were also found on mouse basophils, but not identified.
    [Show full text]
  • Blood and Immunity
    Chapter Ten BLOOD AND IMMUNITY Chapter Contents 10 Pretest Clinical Aspects of Immunity Blood Chapter Review Immunity Case Studies Word Parts Pertaining to Blood and Immunity Crossword Puzzle Clinical Aspects of Blood Objectives After study of this chapter you should be able to: 1. Describe the composition of the blood plasma. 7. Identify and use roots pertaining to blood 2. Describe and give the functions of the three types of chemistry. blood cells. 8. List and describe the major disorders of the blood. 3. Label pictures of the blood cells. 9. List and describe the major disorders of the 4. Explain the basis of blood types. immune system. 5. Define immunity and list the possible sources of 10. Describe the major tests used to study blood. immunity. 11. Interpret abbreviations used in blood studies. 6. Identify and use roots and suffixes pertaining to the 12. Analyse several case studies involving the blood. blood and immunity. Pretest 1. The scientific name for red blood cells 5. Substances produced by immune cells that is . counteract microorganisms and other foreign 2. The scientific name for white blood cells materials are called . is . 6. A deficiency of hemoglobin results in the disorder 3. Platelets, or thrombocytes, are involved in called . 7. A neoplasm involving overgrowth of white blood 4. The white blood cells active in adaptive immunity cells is called . are the . 225 226 ♦ PART THREE / Body Systems Other 1% Proteins 8% Plasma 55% Water 91% Whole blood Leukocytes and platelets Formed 0.9% elements 45% Erythrocytes 10 99.1% Figure 10-1 Composition of whole blood.
    [Show full text]
  • Primary Blood and Immune Cells for Research
    Primary blood and immune cells for research Lonza Walkersville, Inc. | © 2019 Agenda • Introduction and hematopoiesis • Products by tissue type • Tissue sources - overview • Applications • Donor variety • Support and resources Lonza Walkersville, Inc. | © 2019 2 Not every cell will work in your assay… Discover our expanded portfolio of blood and immune cells More than cells – support for wide ranging 01 Highest quality blood tissue and cells 05 workflows including transfection using Lonza’s Nucleofector™ Technology 02 Unmatched donor and cell variety 06 Global supply reach X-VIVO™ Serum-free Hematopoietic Cell Culture Full donor consent and IRB-approved collection 03 07 Media – with cell guarantee* facilities 04 World-class scientific support team 08 Certificate of Analysis, SDS in multiple languages * Lonza guarantees the performance of Clonetics™/Poietics™ Cells only if appropriate Clonetics™/Poietics™ Media and Reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support. Lonza Walkersville, Inc. | © 2019 3 The hematopoietic system From stem cells to T cells Stem Cell Lymphoid Progenitor Myeloid Progenitor Monocyte Basophil Eosinophil Neutrophil B cell T cell Natural Killer Dendritic Cell Macrophage Lonza Walkersville, Inc.
    [Show full text]
  • Regulatory T Cells Do Not Suppress Rather Activate Human Basophils by IL-3 and STAT5-Dependent Mechanisms Mrinmoy Das, Emmanuel Stephen-Victor, Jagadeesh Bayry
    Regulatory T cells do not suppress rather activate human basophils by IL-3 and STAT5-dependent mechanisms Mrinmoy Das, Emmanuel Stephen-Victor, Jagadeesh Bayry To cite this version: Mrinmoy Das, Emmanuel Stephen-Victor, Jagadeesh Bayry. Regulatory T cells do not suppress rather activate human basophils by IL-3 and STAT5-dependent mechanisms. OncoImmunology, Taylor & Francis, 2020, 9, pp.1773193. 10.1080/2162402X.2020.1773193. inserm-02879887 HAL Id: inserm-02879887 https://www.hal.inserm.fr/inserm-02879887 Submitted on 24 Jun 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Distributed under a Creative Commons Attribution - NonCommercial| 4.0 International License OncoImmunology ISSN: (Print) (Online) Journal homepage: https://www.tandfonline.com/loi/koni20 Regulatory T cells do not suppress rather activate human basophils by IL-3 and STAT5-dependent mechanisms Mrinmoy Das , Emmanuel Stephen-Victor & Jagadeesh Bayry To cite this article: Mrinmoy Das , Emmanuel Stephen-Victor & Jagadeesh Bayry (2020) Regulatory T cells do not suppress rather activate human basophils by IL-3 and STAT5-dependent mechanisms, OncoImmunology, 9:1, 1773193, DOI: 10.1080/2162402X.2020.1773193 To link to this article: https://doi.org/10.1080/2162402X.2020.1773193 © 2020 The Author(s).
    [Show full text]
  • Extracellular DNA Traps by Basophils Independent Formation Of
    NADPH Oxidase−Independent Formation of Extracellular DNA Traps by Basophils Mahbubul Morshed, Ruslan Hlushchuk, Dagmar Simon, Andrew F. Walls, Kazushige Obata-Ninomiya, Hajime This information is current as Karasuyama, Valentin Djonov, Alexander Eggel, Thomas of September 26, 2021. Kaufmann, Hans-Uwe Simon and Shida Yousefi J Immunol 2014; 192:5314-5323; Prepublished online 25 April 2014; doi: 10.4049/jimmunol.1303418 Downloaded from http://www.jimmunol.org/content/192/11/5314 Supplementary http://www.jimmunol.org/content/suppl/2014/04/25/jimmunol.130341 Material 8.DCSupplemental http://www.jimmunol.org/ References This article cites 43 articles, 14 of which you can access for free at: http://www.jimmunol.org/content/192/11/5314.full#ref-list-1 Why The JI? Submit online. • Rapid Reviews! 30 days* from submission to initial decision by guest on September 26, 2021 • No Triage! Every submission reviewed by practicing scientists • Fast Publication! 4 weeks from acceptance to publication *average Subscription Information about subscribing to The Journal of Immunology is online at: http://jimmunol.org/subscription Permissions Submit copyright permission requests at: http://www.aai.org/About/Publications/JI/copyright.html Email Alerts Receive free email-alerts when new articles cite this article. Sign up at: http://jimmunol.org/alerts The Journal of Immunology is published twice each month by The American Association of Immunologists, Inc., 1451 Rockville Pike, Suite 650, Rockville, MD 20852 Copyright © 2014 by The American Association of Immunologists, Inc. All rights reserved. Print ISSN: 0022-1767 Online ISSN: 1550-6606. The Journal of Immunology NADPH Oxidase–Independent Formation of Extracellular DNA Traps by Basophils Mahbubul Morshed,* Ruslan Hlushchuk,† Dagmar Simon,‡ Andrew F.
    [Show full text]