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Primary Blood and Immune Cells for Research

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Primary Blood and Immune Cells for Research Primary blood and immune cells for research Lonza Walkersville, Inc. | © 2019 Agenda • Introduction and hematopoiesis • Products by tissue type • Tissue sources - overview • Applications • Donor variety • Support and resources Lonza Walkersville, Inc. | © 2019 2 Not every cell will work in your assay… Discover our expanded portfolio of blood and immune cells More than cells – support for wide ranging 01 Highest quality blood tissue and cells 05 workflows including transfection using Lonza’s Nucleofector™ Technology 02 Unmatched donor and cell variety 06 Global supply reach X-VIVO™ Serum-free Hematopoietic Cell Culture Full donor consent and IRB-approved collection 03 07 Media – with cell guarantee* facilities 04 World-class scientific support team 08 Certificate of Analysis, SDS in multiple languages * Lonza guarantees the performance of Clonetics™/Poietics™ Cells only if appropriate Clonetics™/Poietics™ Media and Reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support. Lonza Walkersville, Inc. | © 2019 3 The hematopoietic system From stem cells to T cells Stem Cell Lymphoid Progenitor Myeloid Progenitor Monocyte Basophil Eosinophil Neutrophil B cell T cell Natural Killer Dendritic Cell Macrophage Lonza Walkersville, Inc. | © 2019 4 Blood sources Peripheral Blood Mobilized Peripheral Bone Marrow Whole Blood Cord Blood (Leukopak) Blood (Leukopak) • The source of hematopoiesis • The result of leukapheresis: • Donor is treated with drug • Collected by standard • Obtained from the umbilical (the process by which blood a specific type of apheresis which “mobilizes” cells from venipuncture cord during birth cells are formed) in which white blood cells the bone marrow to the • Various volumes, containers, • Valuable for obtaining cells • Up to 100mL per donor can are separated from the rest peripheral blood for easier anticoagulants available in a more naïve state be obtained of the blood components collection, and in greater quantities • Multiple treatment regimens available Availability Availability Availability Availability Availability • Fresh whole bone marrow • fresh leukopaks in multiple • fresh mobilized leukopaks • Whole blood • source for isolated • source for characterized cells sizes (volume or total cell • source for large quantities of • Serum, plasma cryopreserved CD34+ count) Stem/Progenitor cells including mesenchymal stem CD34+ stem/progenitor cells • Erythrocytes cells, CD34+ stem/progenitor • Source for characterized cells, and mononuclear cells immune cells Lonza Walkersville, Inc. | © 2019 5 Donor variety Robust programs to find exactly the cells you need FEATURES BENEFITS • All donations from healthy, consenting donors following IRB- • Access the specific donor attributes you require for your approved protocols experiments • Donors from multiple geographic regions in the US with • Choose multiple donors to account for population diversity different ethnicities (west coast, northeast, mid-atlantic) • Obtain cells from a specific donor over time* • Dedicated teams to manage, recruit, recall, and support donors • Compliance with ethical and regulatory guidelines • “Made to order” requests are available (select your specific donor attributes) • Start your experiments immediately with in-stock cryopreserved cells • Large donor variety of select cryopreserved cells in stock and ready to ship * Every effort within ethical and IRB approved limits will be made to schedule specific donors for recall, but no guarantee can be made regarding availability Lonza Walkersville, Inc. | © 2019 6 Flexible and robust sourcing Donor variety Assays Tissue variety Cell variety Lonza Walkersville, Inc. | © 2019 7 Products by tissue type Lonza Walkersville, Inc. | © 2019 Peripheral blood leukopak Blood enriched for leukocytes APHERESIS PROCESS Process Leukopaks are the end product from leukapheresis, a • Your whole blood is process by which donors supply blood enriched for 01 collected through an leukocytes (white blood cells). intravenous tube one arm. • Collections are performed using the Spectra Optia® Apheresis System, from healthy, consenting adults, and following IRB-approved protocols. • Mononucleated cell-rich blood is continuously collected into a sterile bag using ACD-A as an anticoagulant The cells are • Roughly 12 donor blood volumes are processed for a 03 full procedure collected for storage. • Volume-based leukopaks : full / half / quarter The blood passes through The remainder of the an apheresis machine, blood is returned through Minimum Cell Count-based leukopaks: 10B/5B/2.5B 02 04 • which separates the cells an intravenous tube in the • Donor attributes may be selected to suit experimental from the blood. other arm. design • Donors are compensated for time and effort per NIH CD3+ CD4+ T CD8+ T CD14+ CD19+ B Granulo- CD34+ NK Cells guidelines Pan T Cells Cells Mono Cells cytes Typical % < 0.01% 60% 32% 23% 11% 7% 5% 10% Lonza Walkersville, Inc. | © 2019 9 Peripheral blood products Leukopaks Available peripheral blood products Fresh Leukopaks • Full/half/quarter pak Direct shipment • Volume or cell count based Fresh Leukopak Leukapheresis is the Isolated Cells from Peripheral Blood – process used to collect Cells are isolated available as cyro cells and fresh cells (in US) blood enriched for following rigorous leukocytes (white blood SOPs and robust • PBMCs • Regulatory T Cells cells) QC/QA processes • CD14+ Monocytes • Naïve T Cells • CD4+ T Cells • B Cells • CD8+ T Cells • NK Cells • Memory T Cells • Dendritic Cells Lonza Walkersville, Inc. | © 2019 10 Mobilized Leukopak Obtain large quantities of CD34+ hematopoietic stem cells Mobilized leukopaks are the end product from the Regimen description leukapheresis from donors pre-treated with a mobilization drug (Neupogen® or Mozobil®) Neupogen® dosed at 10μg/kg/day 2 collection days • Mobilization drug causes cells in the bone marrow to be “mobilized” RegF for 5 days leading up to collection available (day 5 and day into the circulating peripheral blood. Easier to obtain blood via on day 5. 6) venipuncture compared to bone marrow aspiration. Additionally, up to 10x more CD34+ cells can be obtained from a mobilized leukopak Neupogen® dosed at 10μg/kg/day vs bone marrow aspiration 2 collection days for 5 days leading up to collection. RegH available (day 5 and day Mozobil® dosed at 240 μg/kg/day 6) Donors are healthy, consenting adults, and all protocols are on days 4 and 5 IRB approved. Mozobil® dosed at 20mg/mL, 1.2 MozA Single collection on day 5 mL single dose Multiple treatment regimens available (see table to right) CD34+ CD3+ CD4+ T CD8+ T CD14+ CD19+ Granulo- NK Cells Large quantities of CD34+ HSCs and CD14+ Monocytes can be HSCs Pan T Cells Cells Mono B Cells cytes obtained from mobilized leukopaks Typical % 1% 15% 10% 8% 25% 5% 5% 40% • Neupogen is a registered trademark of Amgen Inc. Mozobil is a registered trademark of Genzyme Corporation Lonza Walkersville, Inc. | © 2019 11 Mobilized Blood Products Mobilized leukopaks Available Mobilized Blood Products Leukapheresis is Fresh Mobilized Bone Marrow performed Leukopak Fresh Mobilized Leukopaks • Available as full leukopaks • Multiple treatment options • RegF • RegH • MozA Donor treated with mobilization agent Isolated Cells from Mobilized Blood • CD34+ Stem/Progenitor Cells • CD14+ Monocytes Cells are isolated following rigorous SOPs Apheresis and robust QC/QA processes Lonza Walkersville, Inc. | © 2019 12 Bone Marrow Products Available Bone Marrow Products Bone Marrow Aspiration Fresh Bone Marrow Biopsy needle • Multiple Volumes, up to 100mL per donor DONOR Skin Fresh Bone Marrow Bone Bone marrow Isolated Cells from Bone Marrow • Mesenchymal Stem Cells • CD34+ Stem/Progenitor Cells • Mononuclear Cells Cells are isolated following rigorous SOPs and robust QC/QA processes Lonza Walkersville, Inc. | © 2019 13 Cord Blood Products Available Cord Blood Products Isolated Cells from Cord Blood Cells are isolated • CD34+ Stem/Progenitor Cells • CD4+ T Cells following rigorous • Mononuclear Cells • CD8+ T Cells SOPs and robust Baby is born with umbilical cord • CD14+ Monocytes 01 QC/QA processes • Naïve T Cells and placenta attached. • CD3+ T Cells • NK Cells After the cord is tied and cut, 02 some blood is left in the blood vessels of the placenta and cord. This cord bleed is extracted 03 from the umbilical cord using a special collection bag. Lonza Walkersville, Inc. | © 2019 14 Whole blood products Available Whole Blood Products Fresh Whole Blood • Whole blood in • Multiple container types • Multiple anticoagulants • Multiple volumes • Plasma • Serum • Platelets • Erythrocytes Lonza Walkersville, Inc. | © 2019 15 Applications Lonza Walkersville, Inc. | © 2019 Key applications / research areas Regenerative medicine (MSCs, Cell & Gene Therapy (T Cells, CD34+ stem cells) Dendritic Cells, NK Cells) Immunogenicity screening (PBMCs, Humanized mice (CD34+ Stem Monocytes, Fresh Blood) Cells / PBMCs) Immuno-oncology (T Cells, B Cells) Drug Clearance (PBMCs) Large-quantity cell isolation CRISPR (T Cells, NK Cells) (Leukopaks) STING (T Cells) Lonza Walkersville, Inc. | © 2019 17 Resources • For additional product information and pricing visit: https://bioscience.lonza.com/hematopoietic-products • Find citations
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