DiMethyl- H4-K20 pAb Cat. No.: 18-634

DiMethyl--K20 pAb

Dot-blot analysis of all sorts of methylation peptides using Immunohistochemistry of paraffin-embedded rat testis DiMethyl-Histone H4-K20 antibody (18-634). using DiMethyl-Histone H4-K20 antibody (18-634) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of 293T cells using DiMethyl-Histone H4-K20 antibody (18-634). Blue: DAPI Immunohistochemistry of paraffin-embedded mouse testis for nuclear staining. using DiMethyl-Histone H4-K20 antibody (18-634) at dilution of 1:200 (40x lens).

Specifications

HOST SPECIES: Rabbit

September 30, 2021 1 https://www.prosci-inc.com/dimethyl-histone-h4-k20-pab-18-634.html SPECIES REACTIVITY: Human, Mouse, Rat

A synthetic methylated peptide corresponding to residues surrounding K20 of human IMMUNOGEN: histone H4

TESTED APPLICATIONS: ChIP, IF, IHC, IP, WB

WB: ,1:500 - 1:2000

IHC: ,1:50 - 1:200

IF: ,1:50 - 1:200 APPLICATIONS: IP: ,1:50 - 1:200

ChIP: ,1:20 - 1:100

CHIP seq: ,1:20 - 1:100

POSITIVE CONTROL: 1) HeLa

2) H4

PREDICTED MOLECULAR Observed: 13kDa WEIGHT:

Properties

PURIFICATION: Affinity purification

CLONALITY: Polyclonal

ISOTYPE: IgG

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles.

Additional Info

OFFICIAL SYMBOL: H4K20me2

H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G, H4FG, HIST1H4D, H4/B, H4FB, HIST1H4E, H4/J, H4FJ, HIST1H4F, H4/C, H4FC, HIST1H4H, H4/H, H4FH, HIST1H4I, H4/M, ALTERNATE NAMES: H4FM, HIST1H4J, H4/E, H4FE, HIST1H4K, H4/D, H4FD, HIST1H4L, H4/K, H4FK, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, HIST2H4B, H4/O, H4FO, HIST4H4, Histone H4H4FA

GENE ID: 8370

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

September 30, 2021 2 https://www.prosci-inc.com/dimethyl-histone-h4-k20-pab-18-634.html Background and References

Histones are basic nuclear that are responsible for the structure of the chromosomal fiber in . This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core (H2A, H2B, H3, and H4). The fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the to form BACKGROUND: higher order chromatin structures. This is intronless and encodes a replication- dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on 1. This gene is one of four histone in the cluster that are duplicated; this record represents the centromeric copy.

ANTIBODIES FOR RESEARCH USE ONLY.

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September 30, 2021 3 https://www.prosci-inc.com/dimethyl-histone-h4-k20-pab-18-634.html