Acetyl- H4-K12 pAb Cat. No.: 15-437

Acetyl--K12 pAb

Immunohistochemistry of paraffin- embedded rat ovary using Acetyl-Histone Immunohistochemistry of paraffin-embedded mouse testis using H4-K12 antibody (15-437) at dilution of 1:200 Acetyl-Histone H4-K12 antibody (15-437) at dilution of 1:200 (40x lens). (40x lens).

Immunohistochemistry of paraffin- Immunofluorescence analysis of C6 cells using Acetyl-Histone H4-K12 embedded mouse spinal cord using Acetyl- antibody (15-437) at dilution of 1:100.C6 cells were treated by TSA (1 Histone H4-K12 antibody (15-437) at dilution uM) at 37? for 18 hours. Blue: DAPI for nuclear staining. of 1:200 (40x lens).

September 27, 2021 1 https://www.prosci-inc.com/acetyl-histone-h4-k12-pab-15-437.html Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H4-K12 antibody (15-437) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37? for 18 hours. Blue: DAPI for nuclear staining.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human, Mouse

IMMUNOGEN: A synthetic acetylated peptide around K12 of human Histone H4 (NP_001029249.1).

TESTED APPLICATIONS: IF, IHC, WB

WB: ,1:500 - 1:2000

APPLICATIONS: IHC: ,1:50 - 1:200

IF: ,1:50 - 1:200

Properties

PURIFICATION: Affinity purification

CLONALITY: Polyclonal

ISOTYPE: IgG

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles.

Additional Info

OFFICIAL SYMBOL: H4K12ac

September 27, 2021 2 https://www.prosci-inc.com/acetyl-histone-h4-k12-pab-15-437.html H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G, H4FG, HIST1H4D, H4/B, H4FB, HIST1H4E, H4/J, H4FJ, HIST1H4F, H4/C, H4FC, HIST1H4H, H4/H, H4FH, HIST1H4I, H4/M, ALTERNATE NAMES: H4FM, HIST1H4J, H4/E, H4FE, HIST1H4K, H4/D, H4FD, HIST1H4L, H4/K, H4FK, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, HIST2H4B, H4/O, H4FO, HIST4H4, Histone H4H4FA

GENE ID: 8366

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

Histones are basic nuclear that are responsible for the structure of the chromosomal fiber in . Two molecules of each of the four core (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called . The linker histone, H1, interacts with BACKGROUND: linker DNA between nucleosomes and functions in the compaction of into higher order structures. This is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on 6.

ANTIBODIES FOR RESEARCH USE ONLY.

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September 27, 2021 3 https://www.prosci-inc.com/acetyl-histone-h4-k12-pab-15-437.html