DiMethyl-Histone H4-K20 pAb Cat. No.: 18-634 DiMethyl-Histone H4-K20 pAb Dot-blot analysis of all sorts of methylation peptides using Immunohistochemistry of paraffin-embedded rat testis DiMethyl-Histone H4-K20 antibody (18-634). using DiMethyl-Histone H4-K20 antibody (18-634) at dilution of 1:200 (40x lens). Immunofluorescence analysis of 293T cells using DiMethyl-Histone H4-K20 antibody (18-634). Blue: DAPI Immunohistochemistry of paraffin-embedded mouse testis for nuclear staining. using DiMethyl-Histone H4-K20 antibody (18-634) at dilution of 1:200 (40x lens). Specifications HOST SPECIES: Rabbit September 30, 2021 1 https://www.prosci-inc.com/dimethyl-histone-h4-k20-pab-18-634.html SPECIES REACTIVITY: Human, Mouse, Rat A synthetic methylated peptide corresponding to residues surrounding K20 of human IMMUNOGEN: histone H4 TESTED APPLICATIONS: ChIP, IF, IHC, IP, WB WB: ,1:500 - 1:2000 IHC: ,1:50 - 1:200 IF: ,1:50 - 1:200 APPLICATIONS: IP: ,1:50 - 1:200 ChIP: ,1:20 - 1:100 CHIP seq: ,1:20 - 1:100 POSITIVE CONTROL: 1) HeLa 2) H4 protein PREDICTED MOLECULAR Observed: 13kDa WEIGHT: Properties PURIFICATION: Affinity purification CLONALITY: Polyclonal ISOTYPE: IgG CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles. Additional Info OFFICIAL SYMBOL: H4K20me2 H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G, H4FG, HIST1H4D, H4/B, H4FB, HIST1H4E, H4/J, H4FJ, HIST1H4F, H4/C, H4FC, HIST1H4H, H4/H, H4FH, HIST1H4I, H4/M, ALTERNATE NAMES: H4FM, HIST1H4J, H4/E, H4FE, HIST1H4K, H4/D, H4FD, HIST1H4L, H4/K, H4FK, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, HIST2H4B, H4/O, H4FO, HIST4H4, Histone H4H4FA GENE ID: 8370 USER NOTE: Optimal dilutions for each application to be determined by the researcher. September 30, 2021 2 https://www.prosci-inc.com/dimethyl-histone-h4-k20-pab-18-634.html Background and References Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form BACKGROUND: higher order chromatin structures. This gene is intronless and encodes a replication- dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 30, 2021 3 https://www.prosci-inc.com/dimethyl-histone-h4-k20-pab-18-634.html.