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Edited by John Cairns, Cold Spring Harbor Laboratory; Gunther S. Stent, University of California, Berkeley; James D. Watson, Harvard University his landmark collection of essays, written by pioneers in the field of molecular biology, was first published T in 1966 as a 60th birthday tribute to Max Delbrück, a formative influence on many of today’s leading sci- entists. The book served as a valuable historical record as well as a remarkable portrait of a small, pioneering, close- knit scientific community that focused intensely on the most important questions in biology. This centennial edi- tion reflects a more personal side of Delbrück, and includes a series of photographs from his family’s albums. “Delbrück had been a kind of Gandhi of biology...It was his extraordinary personality that made him the spiritu- al force which affected the scientific and personal lives of so many people.” —Gunther S. Stent 2007, 394 pp., illus., timeline, photo gallery Hardcover $29 ISBN 978-087969800-3 CONTENTS Note from the Publisher The Injection of DNA into Cells Bacterial Conjugation Quantitatitve Tumor Virology Preface to the First Edition by Phage Elie L. Wollman H. Rubin John Cairns, Gunther S. Stent, James A.D. Hershey Story and Structure of the The Natural Selection Theory D. Watson Transfer of Parental Material to λ Transducing Phage of Antibody Formation; Ten Preface to the Expanded Edition Progeny J. Weigle Years Later John Cairns Lloyd M. Kozloff V. DNA Niels K. Jerne Electron Microscopy of Developing Growing Up in the Phage Group Cybernetics of the Insect I. ORIGINS OF MOLECULAR Optomotor Response BIOLOGY Bacteriophage J.D. Watson Edward Kellenberger Werner E. Reichardt Introduction: Waiting for Demonstration of the Teminal Redundancy, or All’s the Paradox III. PHAGE GENETICS Semiconservative Mode of Well that Ends Well Gunther S. Stent Phenotypic Mixing DNA Duplication George Streisinger APhysicist Looks at Biology Aaron Novick Matthew Meselson and Max Delbrück Mating Theory Franklin W. Stahl VII. REPRINTS Biochemical Genetics: Some N. Visconti The Autoradiography of DNA How Molecular Biology Started Recollections On the Physical Basis of Genetic John Cairns John C. Kendrew George W. Beadle Structure in Bacteriophage Φx: Multum in Parvo Reprinted from Scientific American, March 1967 The Target Theory A.D. Kaiser Robert L. Sinsheimer K.G. Zimmer Adventures in the rII Region The Relation between Nuclear and That Was the Molecular Biology That Was High Energy Phosphate Bonds: Seymour Benzer Cellular Division in Escherichia coli Ole Maaløe Gunther S. Stent Optional or Obligatory? Conditional Lethals Reprinted from Science, April 26, Herman M. Kalckar R.S. Edgar VI. RAMIFICATIONS OF 1968 MOLECULAR BIOLOGY II. THE PHAGE RENAISSANCE IV. BACTERIAL GENETICS Max Delbrück, 1906—1981 The Mammalian Cell Bacteriophage: One-Step Growth Mutations of Bacteria and of Gunther S. Stent T.T. Puck Emory L. Ellis Bacteriophage Reprinted from Genetics, May 1982 S.E. Luria The Plaque Technique and the Electron Microscopy of Phages Timeline Thomas F. Anderson Gene, Transforming Principle, Development of Quantitative and DNA Animal Virology Photo Gallery The Eclipse in the Bacteriophage Renato Dulbecco Life Cycle Rollin D. Hotchkiss A.H. Doermann Sexual Differentiation in Bacteria The Prophage and I William Hayes André Lwoff To order or request additional information, please visit our Web site or: Call: 1-800-843-4388 (Continental US and Canada) 516-422-4100 (All other locations) FAX: 516-422-4097 E-mail: [email protected] Write: Cold Spring Harbor Laboratory Press, 500 Sunnyside Blvd., Woodbury, NY 11797-2924 By Georgina Ferry Few scientists have thought more deeply about the nature of their calling and its impact on humanity than Max Perutz (1914-2002). Born in Vienna, Jewish by descent, lapsed Catholic by religion, he came to Cambridge in 1936, to join the lab of the legendary Communist thinker J.D. Bernal. There he began to explore the structures of the molecules that hold the secret of life. In 1940, he was interned and deported to Canada as an enemy alien, only to be brought back and set to work on a bizarre top secret war project. In 1947, he founded the small research group in which Francis Crick and James Watson discovered the structure of DNA: under his leadership it grew to become the world-famous Laboratory for Molecular Biology. Max himself explored the protein hemoglobin and his work, which won him a Nobel Prize in 1962, launched a new era of medicine, heralding today’s astonishing advances in the genetic basis of disease. Max Perutz’s story, wonderfully told by Georgina Ferry, brims with life. It has the zest of an adventure novel and is full of extraordinary characters. Max was demanding, passionate and driven but also humorous, compassionate and loving. Small in stature, he became a fearless mountain climber; drawing on his own experience as a refugee, he argued fearlessly for human rights; he could be ruthless but had a talent for friendship. An articulate and engaging advocate of science, he found new problems to engage his imagination until weeks before he died aged 88. About the author: Georgina Ferry is a former staff editor on New Scientist, and contributor to BBC Radio 4’s Science Now. Her books include the acclaimed biography Dorothy Hodgkin: A Life (1998); The Common Thread (2002, with Sir John Sulston) and A Computer Called LEO (2003). She lives in Oxford. 2007, 352 pp., illus., glossary, index Hardcover $39 ISBN 978-087969785-3 CONTENTS List of illustrations 5 Mountains and Mahomet 11Health and disease Preface and acknowledgements 6 How hemoglobin was not solved 12Truth always wins 1 Scenes from a Vienna childhood 7 Annus mirabilis Select bibliography 2 ‘It was Cambridge that made me’ 8 In search of solutions Notes 3 ‘The most dangerous characters of all’ 9 A structure for science – the LMB Glossary 4 Home and homeland 10The breathing molecule Index To order or request additional information, please visit our Web site or: Call: 1-800-843-4388 (Continental US and Canada) 516-422-4100 (All other locations) FAX: 516-422-4097 E-mail: [email protected] Write: Cold Spring Harbor Laboratory Press, 500 Sunnyside Blvd., Woodbury, NY 11797-2924 Gene Knockdown guaranteed. ™ HuSH-29 Downregulation of HIF1A shRNA Expression Expression by HuSH Contructs Plasmids • All shRNA vectors delivered as 5 ug of highly purifi ed, transfection-ready plasmid DNA. 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