Edited by John Cairns, Cold Spring Harbor Laboratory; Gunther S. Stent, University of California, Berkeley; James D. Watson, Harvard University his landmark collection of essays, written by pioneers in the field of molecular biology, was first published T in 1966 as a 60th birthday tribute to Max Delbrück, a formative influence on many of today’s leading sci- entists. The book served as a valuable historical record as well as a remarkable portrait of a small, pioneering, close- knit scientific community that focused intensely on the most important questions in biology. This centennial edi- tion reflects a more personal side of Delbrück, and includes a series of photographs from his family’s albums. “Delbrück had been a kind of Gandhi of biology...It was his extraordinary personality that made him the spiritu- al force which affected the scientific and personal lives of so many people.” —Gunther S. Stent 2007, 394 pp., illus., timeline, photo gallery Hardcover $29 ISBN 978-087969800-3

CONTENTS Note from the Publisher The Injection of DNA into Cells Bacterial Conjugation Quantitatitve Tumor Virology Preface to the First Edition by Phage Elie L. Wollman H. Rubin John Cairns, Gunther S. Stent, James A.D. Hershey Story and Structure of the The Natural Selection Theory D. Watson Transfer of Parental Material to λ Transducing Phage of Antibody Formation; Ten Preface to the Expanded Edition Progeny J. Weigle Years Later John Cairns Lloyd M. Kozloff V. DNA Niels K. Jerne Electron Microscopy of Developing Growing Up in the Phage Group Cybernetics of the Insect I. ORIGINS OF MOLECULAR Optomotor Response BIOLOGY Bacteriophage J.D. Watson Edward Kellenberger Werner E. Reichardt Introduction: Waiting for Demonstration of the Teminal Redundancy, or All’s the Paradox III. PHAGE GENETICS Semiconservative Mode of Well that Ends Well Gunther S. Stent Phenotypic Mixing DNA Duplication George Streisinger APhysicist Looks at Biology Aaron Novick Matthew Meselson and Max Delbrück Mating Theory Franklin W. Stahl VII. REPRINTS Biochemical Genetics: Some N. Visconti The Autoradiography of DNA How Molecular Biology Started Recollections On the Physical Basis of Genetic John Cairns John C. Kendrew George W. Beadle Structure in Bacteriophage Φx: Multum in Parvo Reprinted from Scientific American, March 1967 The Target Theory A.D. Kaiser Robert L. Sinsheimer K.G. Zimmer Adventures in the rII Region The Relation between Nuclear and That Was the Molecular Biology That Was High Energy Phosphate Bonds: Seymour Benzer Cellular Division in Escherichia coli Ole Maaløe Gunther S. Stent Optional or Obligatory? Conditional Lethals Reprinted from Science, April 26, Herman M. Kalckar R.S. Edgar VI. RAMIFICATIONS OF 1968 MOLECULAR BIOLOGY II. THE PHAGE RENAISSANCE IV. BACTERIAL GENETICS Max Delbrück, 1906—1981 The Mammalian Cell Bacteriophage: One-Step Growth Mutations of Bacteria and of Gunther S. Stent T.T. Puck Emory L. Ellis Bacteriophage Reprinted from Genetics, May 1982 S.E. Luria The Plaque Technique and the Electron Microscopy of Phages Timeline Thomas F. Anderson Gene, Transforming Principle, Development of Quantitative and DNA Animal Virology Photo Gallery The Eclipse in the Bacteriophage Renato Dulbecco Life Cycle Rollin D. Hotchkiss A.H. Doermann Sexual Differentiation in Bacteria The Prophage and I William Hayes André Lwoff

To order or request additional information, please visit our Web site or: Call: 1-800-843-4388 (Continental US and Canada) 516-422-4100 (All other locations) FAX: 516-422-4097 E-mail: [email protected] Write: Cold Spring Harbor Laboratory Press, 500 Sunnyside Blvd., Woodbury, NY 11797-2924 By Georgina Ferry Few scientists have thought more deeply about the nature of their calling and its impact on humanity than Max Perutz (1914-2002). Born in Vienna, Jewish by descent, lapsed Catholic by religion, he came to Cambridge in 1936, to join the lab of the legendary Communist thinker J.D. Bernal. There he began to explore the structures of the molecules that hold the secret of life. In 1940, he was interned and deported to Canada as an enemy alien, only to be brought back and set to work on a bizarre top secret war project. In 1947, he founded the small research group in which Francis Crick and James Watson discovered the structure of DNA: under his leadership it grew to become the world-famous Laboratory for Molecular Biology. Max himself explored the protein hemoglobin and his work, which won him a Nobel Prize in 1962, launched a new era of medicine, heralding today’s astonishing advances in the genetic basis of disease. Max Perutz’s story, wonderfully told by Georgina Ferry, brims with life. It has the zest of an adventure novel and is full of extraordinary characters. Max was demanding, passionate and driven but also humorous, compassionate and loving. Small in stature, he became a fearless mountain climber; drawing on his own experience as a refugee, he argued fearlessly for human rights; he could be ruthless but had a talent for friendship. An articulate and engaging advocate of science, he found new problems to engage his imagination until weeks before he died aged 88. About the author: Georgina Ferry is a former staff editor on New Scientist, and contributor to BBC Radio 4’s Science Now. Her books include the acclaimed biography Dorothy Hodgkin: A Life (1998); The Common Thread (2002, with Sir John Sulston) and A Computer Called LEO (2003). She lives in Oxford. 2007, 352 pp., illus., glossary, index Hardcover $39 ISBN 978-087969785-3

CONTENTS List of illustrations 5 Mountains and Mahomet 11Health and disease Preface and acknowledgements 6 How hemoglobin was not solved 12Truth always wins 1 Scenes from a Vienna childhood 7 Annus mirabilis Select bibliography 2 ‘It was Cambridge that made me’ 8 In search of solutions Notes 3 ‘The most dangerous characters of all’ 9 A structure for science – the LMB Glossary 4 Home and homeland 10The breathing molecule Index

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• A new, online source of trusted techniques in molecular and cellular biology • Contains cutting-edge and classic protocols presented step-by-step with cautions and troubleshooting • Frequently updated and annotated • Interactive, customizable, and fully searchable

old Spring Harbor Laboratory is renowned for its teaching of biomedical research techniques. C For decades, participants in its celebrated, hands-on courses and users of its laboratory manuals have gained access to the most authoritative and reliable methods in molecular and cellular biology. Now that access has moved online. Visit Cold Spring Harbor Protocols today and discover a rich, interactive source of new and classic research techniques. The site is fully searchable, with many tools that can be customized by users including topic-based alerting and personal folders. Through a web-based editorial process, users also have the opportunity to add refereed comments to each protocol. Links in the online protocols offer additional resources, and step-by-step instructions print out in a convenient form, complete with mate- rials, cautions, and troubleshooting advice. Each protocol is citable and presented and edited in the style that has made Molecular Cloning, Antibodies, Cells, and many other Cold Spring Harbor manuals essential to the work of scientists worldwide. The current collection of more than 1,000 protocols is continuously expanded, updated, and annotated by the originators and users of the techniques. CSH Protocols is created by Cold Spring Harbor Laboratory Press in association with HighWire Press of Stanford University. Cold Spring Harbor Protocols • Executive Editor: Dr. David Crotty ISSN 1559-6095 / online, monthly • Available exclusively via institutional site license

Subject Coverage Antibodies Molecular Biology Bioinformatics/Genomics Neuroscience Cell Biology Newly Added Protocols Chromatography Plant Biology Computational Biology Polymerase Chain DNA Delivery/Gene Transfer Reaction (PCR) Electrophoresis Proteins and Proteomics Genetics RNA Interference High Throughput Analysis (RNAi)/siRNA Imaging/Microscopy Stem Cells Immunology Transgenic Technology Laboratory Organisms

For pricing information or to request a free trial visit the website or: Phone:1-800-843-4388 (Continental US and Canada) or 516-422-4100 (all other locations) Fax: 516-422-4097 E-mail:[email protected] or WWW Site: www.cshlpress.com Write: Cold Spring Harbor Laboratory Press, 500 Sunnyside Blvd., Woodbury, NY 11797-2924 Chair, Department of Biostatistics and Computational Biology Dana-Farber Cancer Institute And Professor of Biostatistics Harvard School of Public Health

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Cold Spring Harbor Laboratory Meeting Epithelial-Mesenchymal Transition March 17 - 20, 2008 Cold Spring Harbor, New York Developmental/Cell Biologist Abstract Deadline: January 25 Associate/Full Professor Organized by: The Department of Biology at Temple University is expanding Senthil Muthuswamy, John Haley, Raghu Kalluri its research programs and anticipates multiple faculty hires Topics: over each of the next several years. The Department invites applications for an Associate/Full Professor (tenured/tenure- Biology of EMT in development, track) position in the area of Developmental/Cell Biology. We inflammation and fibrosis are especially interested in individuals who are using current Extracellular signaling and cell biology of EMT molecular genetic approaches to study basic mechanisms of developmental pathways and/or cell function. Individuals Intracellular regulators of EMT whose research programs complement and extend the EMT models and stem cells department’s strengths in vertebrate development, Pathobiology of EMT in cancer progression developmental neuroscience, RNA biology, molecular virology, and cancer biology are especially urged to apply. However, Keynotes: Mina Bissell, Robert Weinberg outstanding candidates in other areas also will be given full Chairs/Discussion Leaders: consideration. Applicants are expected to have a significant track record of funded research, as well as teaching at the Thomas Brabletz, Robert Cardiff, John Condeelis, graduate and undergraduate levels. Applicants should submit Steven Dubinett, Rebecca Fitzgerald, Raghu Kalluri, their curriculum vitae, a research program summary, and the Aris Moustakas, Senthil Muthuswamy, Angela Nieto, names and contact information of three references to: Dr. Richard Waring, Developmental/Cell Biologist Search Eric Neilson, Daniel Peeper, Frank Rauscher, Jeff Committee Chair, Department of Biology, Temple University, Rosen, Kathy Svoboda , Erik (Rik) Thompson ,Valerie 1900 North 12th Street, Philadelphia, PA 19122. E-mail: Weaver, Steve Weiss, Jim Woodgett, Liz Williams [email protected]. Review of applications will begin Abstracts are welcomed for selection as talks or posters. A limited number immediately and will continue until the position is filled. of partial stipends are available thanks to OSI Pharmaceuticals Temple University is an equal opportunity, equal access, Cold Spring Harbor Laboratory, Meetings & Courses Program, affirmative action employer committed to achieving a diverse 1 Bungtown Road, Cold Spring Harbor, NY 11724 community (AA, EOE, m/f/d/v). www.cshl.edu/meetings/ [email protected] Ph: (516) 367-8346 By Toby Freedman, Synapsis Search, Portola Valley, California s the world of biotechnology has grown in leaps and bounds, so too have the career opportunities. A But the choices can be daunting. What types of jobs are available? How do you get your foot in the door? What will your job entail if you become a “Preclinical Project Manager” or a “Process Scientist”? What’s the difference between biotech and pharma? Career Opportunities in Biotechnology and Drug Development provides a comprehensive and systematic overview of careers in the life science industry, with all their ups and downs. The author, Toby Freedman, Ph.D., has conducted interviews with hundreds of key players in the industry, who provide first–hand explanations of their day–to–day roles and responsibilities, and offer key insights into how they landed those jobs in the first place. Careers in everything from discovery research to venture capital are covered in detail. Each chapter includes valuable sections on preparing yourself for a prospective career: educational requirements and person- ality characteristics needed; recommendations of books, magazines, and Web site resources; and issues to consider regarding salary and compensation. The book also includes interviewing and job searching tips, as well as suggestions on writing a resume specifically for industry. Career Opportunities in Biotechnology and Drug Development is an essential guide for science graduates and medical, business, legal, high–tech or engineering professionals. With discussions of job security, future trends, and potential career paths, even those already working in industry will find helpful information on how to take advantage of opportunities available within their own companies and elsewhere. This book will help you make wiser and more informed decisions about what role you would like to play in the biotechnology and drug development industry. 2008, 409 pp., illus., index Hardcover $59.00 ISBN 978-087969725-9

CONTENTS PART I: INDUSTRYOVERVIEW: 9. Project Management: The Product 19. Sales: Generating Revenue and Educating LANDING A JOB IN INDUSTRY Development “Orchestra Conductors” Customers 1. The Pros and Cons of Working in Industry: 10. Clinical Development: Developing New 20. Technical Applications and Support: Getting Why Make the Transition? Products to Benefit Human Health Paid to Be the Expert 2. How to Excel in Industry: What to Expect 11. Medical Affairs: Working in the 21. Corporate Communications: and What is Expected of You Post–Approval World Communication between External and 3. So You Want a Job in Biotechnology and 12. Regulatory Affairs: The Final Challenge— Internal Worlds Drug Development...: Finding Your Way In Passing the FDA Test 22. Executive Leadership and Entrepreneurship: 4. The Biotechnology Industry Resume: Putting 13. Quality: Consistently Making Good The Business Builders Your Best Foot Forward Products 23. Law: Providing Legal Advice and Protecting 5. The Informational Interview: Researching 14. Operations: Ensuring that Processes Run Property Your Options Smoothly and Efficiently 24. Health Care Finance: Venture Capital, 6. The Biotechnology and Drug Development 15. Bio/Pharmaceutical Product Development: Institutional Investing, Investment Banking, Industry: An Overview The Chemistry Has to Be Good and Equity Research 16. Life Science Information Management: 25. Management Consulting: The Strategy PART II: CAREER OPPORTUNITIES The Melding of Computer and Advisors IN BIOTECHNOLOGY AND DRUG Biological Sciences 26. Recruiting: The Business of Matchmaking DEVELOPMENT COMPANIES 17. Business and Corporate Development: Index 7. Discovery Research: The Idea Makers Why Big Deals Really Are a Big Deal 8. Preclinical Research: The Bridge between 18. Marketing: Communicating a Discovery Research and Clinical Development Message to Customers

To order or request additional information, please visit our Web site or: Call: 1-800-843-4388 (Continental US and Canada) 516-422-4100 (All other locations) FAX: 516-422-4097 E-mail: [email protected] Write: Cold Spring Harbor Laboratory Press, 500 Sunnyside Blvd., Woodbury, NY 11797-2924 Edited by Michael P. Weiner, RainDance Technologies, Inc., Guilford, Connecticut; Stacey B. Gabriel, The Broad Institute, Massachusetts Institute of Technology, Cambridge, and J. Claiborne Stephens, Motif BioSciences, New York enetic Variation: A Laboratory Manual is the first compendium of protocols specifically geared towards G genetic variation studies, and includes thorough discussions on their applications for human and model organ- ism studies. Intended for graduate students and professional scientists in clinical and research settings, it covers the complete spectrum of genetic variation—from SNPs and microsatellites to more complex DNA alterations, includ- ing copy number variation. Written and edited by leading scientists in the field, the early sections of the manual are devoted to study design and generating genotype data, the use of resources such as HapMap and dbSNP, as well as experimental, statistical, and bioinformatic approaches for analyzing the data. The final sections include descrip- tions of genetic variation in model organisms and discussions of recent insights into human genetic ancestry, foren- sics, and human variation. 2007, 793 pp., illus., appendix, index Hardcover $240 ISBN 978-087969779-2 Paperback $165 ISBN 978-087969780-8

CONTENTS 1. Ethical Issues in Human Genetic Research: The 12. Molecular Inversion Probes and Universal Tag 21. Haploview: Visualization and Analysis of SNP Global Experience, K. Arnold and J. van der Walt Arrays: Application to Highplex Targeted SNP Genotype Data, J.C. Barrett Genotyping, G. Karlin-Neumann, M. Sedova, SECTION 1: STUDY DESIGN 22. Considerations for Copy Number Analysis of R. Sapolsky, J. Forman, Y. Wang, M. Moorhead, FFPE Samples, S. Jacobs Introduction and M.Faham 2. Population Choice as a Consideration for 23. Assessing Significance in Genetic Association 13. Whole-Genome Genotyping, S.B. Gabriel and Studies, M.J. Daly Genetic Analysis Study Design, J.C. Stephens M.P. Weiner and M. Bamshad 24. Assessing Human Variation Data for Signatures 3. Power Calculations, D.M. Evans and S. Purcell PART 3: COPY NUMBER AND COMPLEX of Natural Selection, M. Bamshad and VARIATION ANALYSIS 4. Genetic Anaylsis: Moving between Linkage and J.C. Stephens Association, A.V. Smith 14. Comparative Genomic Hybridization to Detect SECTION 4: VARIATION STUDIES IN Variation in the Copy Number of Large DNA 5. NCBI dbSNP Database: Content and Searching, MODEL ORGANISMS Segments, I.N. Holcomb and B.J. Trask M.L. Feolo and S.T. Sherry Introduction 15. Representational Oligonucleotide Microarray 6. Using the HapMap Web Site, A.V. Smith 25. Arabidopsis, Y. Li and J.O. Borevitz Analysis Detection of Genetic Variation, 26. Maize, W.B. Barbazuk, A.-P. Hsia, H.D. Chen, SECTION 2: LABORATORY PROTOCOLS R. Lucito Y. Fu, K. Ohtsu, and P.S. Schnable Introduction 16. Whole-Genome Sampling Analysis to Detect 27. Rice, H. Leung, K.L. McNally, and D. Mackill PART 1: PREPARATION OF DNA AND RNA Copy Number Changes in FFPE Samples, 7. Isolation of Plant DNA for Genotyping Analysis, S. Jacobs 28. The Mouse, C.M. Wade and M.J. Daly N.M. Springer 17. Molecular Inversion Probe Targeted 29. The Rat, E. Cuppen, N. Hübner, H.J. Jacob, 8. Preparing RNA from Plant Tissues, A.-P. Hsia, Genotyping: Application to Copy Number and A.E. Kwitek H.D. Chen, K. Ohtsu, and P.S. Schnable Determination, G. Karlin-Neumann, 30. The Cat, M.J. Lipinski, N. Billings, L.A. Lyons 9. Preparing DNA from Mammalian Sources, M. Sedova, R. Sapolsky, S. Lin, Y. Wang, 31. The Dog, K. Lindblad-Toh and E.A. Ostrander A. Sahota, A.I. Brooks, and J.A. Tischfield M. Moorhead, and M. Faham 32. The Chimpanzee, T.S. Mikkelsen, M.C. Zody, and K. Lindblad-Toh PART 2: SNP VARIATION ANALYSIS 18. Microsatellite Markers for Linkage and Association Studies, J. Gulcher 10. Intermediate-Throughput Laboratory-Scale SECTION 5: INSIGHTS INTO HUMAN Genotyping Solutions, S.J. Macdonald SECTION 3: DATA ANALYSIS VARIATION 11. Intermediate-Throughput Laboratory-Scale Introduction Introduction Genotyping Protocols, E. Cuppen, 19. Considerations for SNP Selection, C. Carlson 33. Genealogical Markers: mtDNA and the Y S.J. Macdonald, C. Ha, P.-Y. Kwok, W.B. 20. Selection and Evaluation of tag-SNPs Using Chromosome, M. Stoneking and M. Kayser Barbazuk, A.-P. Hsia, H.D. Chen, Y. Fu, Tagger and HapMap, P. de Bakker 34. Forensic DNA Testing, J.M. Butler K. Ohtsu, and P.S. Schnable 35. The Human Genome: What Lies Ahead, M.P. Weiner and J.C. Stephens APPENDIX: Cautions INDEX To order or request additional information: Call: 1-800-843-4388 (Continental US and Canada) 516-422-4100 (All other locations) FAX: 516-422-4097 E-mail: [email protected] Write: Cold Spring Harbor Laboratory Press, 500 Sunnyside Blvd., Woodbury, NY 11797-2924 The Home for RNA Scientists

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