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Volume 25 1, number 1,2, 22-26 FEB 07357 July 1989

Platelet-activating factor increases phosphate production and cytosolic free Ca2+ concentrations in cultured rat Kupffer cells

Rory A. Fisher+, Ram V. Sharma and Ramesh C. Bhalla

Departments of ‘Pharmacology and Anatomy, The University of Iowa, College of Medicine, Iowa City, IA 52242, USA

Received 15 April 1989; revised version received 19 May 1989

Platelet-activating factor (PAF) stimulates glycogenolysis in perfused livers but not in isolated hepatocytes [(1984) J. Biol. Chem. 259, 8685586881.PAF-induced glycogenolysis in liver is associated closely with a pronounced constriction of the hepatic vasculature [(1986) J. Biol. Chem. 261,644-649]. These and other observations suggest that PAF stimulates glyco- genolysis in liver indirectly by interactions with cells other than hepatocytes. We have evaluated effects of PAF on hepatic Kupffer cells, which regulate flow through the hepatic sinusoids. Application of PAF to PH]inositol-labeled Kupffer cells produced dose-dependent increases in PH]inositol phosphates with an EC,, value of 4 x lo-i0 M. Increases in production in response to PAF were inhibited by a specific PAF receptor antagonist, SRI 63-675 (2 x lo-’ M), and stimulus of protein kinase C, phorbol 12-myristate 13-acetate (1 x IO-’ M). Measurements of cytosolic free Ca2+ concentrations ([Caz’],) in single Kupffer cells loaded with Fura- demonstrated that application of PAF (2 x low9 M) resulted in significant increases in [Ca2’li. These observations lead us to propose that interactions of PAF with Kupffer cells may result in the hemodynamic and metabolic responses to PAF in liver.

Platelet-activating factor; Phospholipase C; Inositol phosphate; Ca*+; (Liver, Kupffer cell)

1. INTRODUCTION Ca2+ mobilization [2,6,7]. This apparent enigma appears due to degradation of phosphoinositides PAF (I-0-alkyl-2-acetyl-sn-glycero-3-phospho- by phospholipase AS following exposure of hepato- ) is a unique mediator which cytes to PAF [8]. Associated with the glycogeno- exerts potent glycogenolytic actions in perfused rat lytic effect of PAF in perfused livers is a pronounc- livers by mechanisms distinct from a-adrenergic ed constriction of the hepatic vasculature resulting agents or glucagon [l-6]. Most notable among the in several metabolic alterations consistent with differences between PAF and these other glyco- ischemia within hepatic sinusoids [1,3,9]. It is well genolytic agents is the absence of glycogenolytic ac- known that ischemia causes glycogenolysis in liver tions of PAF in isolated hepatocytes [2], the princi- [lo]. Thus, we have suggested that hepatocytes are ple storage site for glycogen in liver. Interestingly, not the primary site of action of PAF in the liver PAF stimulates degradation of phosphatidyl- and that glycogenolysis in response to this inositol bisphosphate in hepatocytes without con- mediator is a result of interactions with the hepatic sequent production of or vasculature leading to vasoconstriction [3,4]. This concept is supported by the absence of glycogeno- Correspondence address: R.A. Fisher, Dept of Pharmacology, lytic responses to pathophysiological concentra- The University of Iowa, College of Medicine, Iowa City, IA tions of PAF in retrograde-perfused livers and liver 52242, USA slices as well as localization studies demonstrating that [3H]PAF binds exclusively to periportal Abbreviations: PAF, platelet-activating factor; [Ca’+]i, free cytosolic Ca2+ concentration; PMA, Ilp-phorbol l@‘-myristate sinusoidal cells and not hepatocytes upon infusion 13cu-acetate; M199, medium 199; BSS, Earl’s balanced salt solu- into perfused livers [ 111. tion It seemed entirely appropriate to investigate

Published by Elsevier Science Publishers B. V. (Biomedical Division) 22 00145793/89/$3.50 0 1989 Federation of European Biochemical Societies Volume 25 1, number 1,2 FEBS LETTERS July 1989 agonist properties of PAF in hepatic Kupffer cells, agonist addition by removing the medium and adding 1 ml of which act as sphincters to regulate flow through the ice-cold methanol. Inositol phosphates were extracted by addi- hepatic sinusoids [12]. Observations in the per- tion of 1.5 ml of chloroform: water (2: 1) and samples of the aqueous phase were analyzed for total labeled inositol phos- fused liver indicate that biological responses to phates by batch elution from Dowex columns as described by PAF may involve mobilization of Ca2+ from a Brown et al. [17]. small non-hepatocyte pool [ 131 and Ca2+ mobiliza- [Ca”]i was measured in individual Kupffer cells using fluo- tion is an important signalling mechanism by which rescent videomicroscopy and digital image analysis of Fura-2- loaded cells as described by Sharma and Bhalla [ 181. These ex- various hormones and mediators regulate cell func- periments were performed with 3 day cultures following incuba- tion. Moreover, it is now well accepted that metab- tion in serum-free Ml99 for 20-24 h. Kupffer cells were loaded olism of inositol can lead to the with Fura- by incubation with Fura_2/acetoxymethyl ester generation of second messengers which regulate (5 x 10m6M) for 45 min at 37°C and then incubated an addi- [Ca2+]i [ 141. In the present communication we have tional 30 min in BSS at 37°C. Results are expressed as means + SE. Significance of dif- performed experiments to explore agonist effects ferences between samples was assessed using analysis of of PAF on inositol lipid hydrolysis and [Ca2+]i in variance and Sheffe’s post hoc analysis. hepatic Kupffer cells. These studies provide the first evidence that PAF exerts agonist effects on 3. RESULTS Kupffer cells at concentrations comparable to those producing biological responses in liver. Fig.1 illustrates the effect of PAF on inositol phosphate production in [3H]inositol-labeled Kupffer cell cultures. Exposure of Kupffer cells to 2. MATERIALS AND METHODS PAF (1 x 10m9M) resulted in a significant increase 2.1. Materials (gcO.01) in inositol phosphate production which PAF (l-O-hexadecyl-2-acetyl-sn-glycero-3-) was not observed in response to a 200-fold higher and lyso-PAF (I-O-hexadecyl-sn-glycero-3-phosphocholine) concentration of lyso-PAF. Incubation with vehi- were purchased from Bachem. myo-[2-‘H]Inositol was obtained cle (saline) alone did not increase [3H]inositol phos- from Amersham. SRI 63-675 was kindly provided by Dr Dean Handley, Sandoz Research Institute. All other chemicals were phates in Kupffer cells above values observed at obtained from Sigma. time 0. Stimulation of Kupffer cell inositol phosphate 2.2. Kupffer cell culture production by PAF was dose-dependent with half- Kupffer cells were isolated from livers of male Sprague- Dawley rats (200-250 g) employing previously described maximal responses occurring at approximately methods [ 151. Briefly, livers were digested by collagenase/pro- 4 x lo-” M PAF (fig.2). The exquisite sensitivity nase perfusion and sinusoidal cells were purified by centrifuga- of Kupffer cells to PAF can be appreciated by the tion on a metrizamide gradient. Cells were cultured in Ml99 small but significant (pcO.05) increase in inositol containing 10% fetal bovine serum (4 x lo6 cells/35 mm phosphate production observed in response to culture dish) at 37°C in a 5% CO2 atmosphere. Culture medium was replaced after overnight incubation resulting in the removal 1 x lo-” M PAF. Maximal responses to PAF were of non-adherent endothelial cells [16] and cultures were used for apparent at 3 x 1O-9 M. At maximal concentra- experiments 1 to 5 days after plating. The purity of these Kupf- tions, PAF increased [3H]inositol phosphates in fer cell cultures was 90-95% as assessed by positive peroxidase Kupffer cells over 700% compared to either saline staining, in agreement with previous histochemical as well as morphological and functional characterizations of these or time 0 (not shown) values. Preliminary ex- cultures [15,16]. For measurements of [Ca’+]r in single Kupffer periments employing HPLC separation of inositol cells, Kupffer cells were cultured on 25 mm glass coverslips. As phosphates have shown that increases in radioac- with plastic culture dishes, Kupffer cells but not endothelial cells tivity in inositol 1,4,5trisphosphate are apparent attached to the glass surface [16]. in Kupffer cells stimulated with PAF (2 x 10m9M) 2.3. Incubations and analyses for 15 s (not shown). All studies of inositol phosphate production in Kupffer cells Activation of platelets by PAF can be inhibited were performed at 37°C. For labeling inositol , cultures by PAF receptor antagonists and activators of pro- were incubated 20-24 h in serum-free Ml99 containing myo- tein kinase C [19-211. Activation of protein kinase [2-‘Hlinositol (l-2 pCi/ml). Cultures were rinsed and incubated in BSS for 60 min and then rinsed and incubated 15 min in BSS C inhibits receptor-stimulated phospholipase C in a containing 5 mM LiCl prior to addition of PAF and other variety of cells and may represent an important agents (time 0). Incubations were terminated 30 min following feedback mechanism for regulating receptor re-

23 Volume 25 1, number 1.2 FEBS LETTERS July 1989

3000 A z ii 2000 E P s

f ‘000 0

Fig.1. Effect of PAF on inositol phosphate production in Fig.3. Effects of SRI 63-675 and PMA on PAF-stimulated in- cultured rat Kupffer cells. Kupffer cells were incubated with ositol phosphate production in cultured rat Kupffer cells. SRI saline, lyso-PAF (2 x lo-’ M) or PAF (1 x 10e9 M) for 30 min 63-675 (2 x lo-’ M) and PMA (1 x 10m7M) were added to before termination of incubation and measurement of inositol Kupffer cells 15 min prior to addition of saline or PAF phosphates as described under section 2. Values shown repre- (2 x low9 M) for 30 min. Incubations and inositol phosphate sent means f SE (n = 3). measurements were performed as described under section 2. Values shown represent means of duplicate experiments. sponses [14]. To characterize PAF receptor re- sponses in Kupffer cells further, PAF-stimulated tion of Kupffer cells with PMA produced increases inositol phosphate production was evaluated in the in [3H]inositol phosphates of approximately 100%. presence of a PAF receptor antagonist (SRI Similar results have been reported in MDCK cells 63-675) [22] and activator of protein kinase C where PMA increased basal levels of inositol (PMA). Fig.3 shows that PAF-stimulated inositol monophosphate but inhibited receptor-mediated phosphate production in Kupffer cells was reduced production of inositol phosphates [23]. to approximately 10% and 35% of control values In view of our findings that PAF receptors are by SRI 63-675 (2 x IO-’ M) and PMA coupled to activation of phosphoinositide phos- (1 x lo-’ M), respectively. Interestingly, incuba- pholipase C in Kupffer cells, preliminary ex- periments were performed to evaluate effects of PAF on [Ca’+]i in Kupffer cells. [Ca’+]i in un- - 4000 b 1 140 z 3000 5^ 120 5 2000 = 100 .z I? 1000 T F. N 80 I 0 (3! 1 0 g 60

rim 40 Saline -11 -10 -9 -8 -7 -6

[PAFlloglO, WI Ii0 2io Fig.2. Dose response of PAF on inositol phosphate production Seconds in cultured rat Kupffer cells. Kupffer cells were incubated with saline or various concentrations of PAF for 30 min before ter- Fig.4. Effect of PAF on [Ca*+]i in an individual rat Kupffer cell. mination of incubation and measurement of inositol phosphates PAF was added to the microscope chamber at time 0 as an equal as described under section 2. Values shown represent means k volume addition to provide a final PAF concentration of SE (n = 3). 2 x 10e9 M. [Ca*+]i was measured as described under section 2.

24 Volume 251, number 1,2 FEBS LETTERS July 1989 stimulated Kupffer cells averaged 62 + 8 nM i.e., 2 x lo-” to 2 x 10e9 M [3,4]. EC& values for (n = 12). Fig.4 illustrates a representative effect of these effects of PAF in perfused livers PAF (2 x 10m9M) on [Ca’+]r in a single Kupffer (-2 x lo-” M) [4] are very similar to values cell. Although maximal increases in [Ca2+]i in re- observed in this study for effects of PAF on Kupf- sponse to PAF exposure were apparent at the first fer cell inositol phosphate production. These sampling period (10 s) in some cells, typically there observations support the concept that Kupffer cells was a delay as shown in fig.4. These variations are a primary site of PAF action in liver. were observed even with different cells on the same Recently, Kuiper et al. [24] reported that PAF coverslip suggesting some cell-cell variability in re- stimulates production of prostaglandin Dz from sponses to PAF. After the peak increase in [Ca’+]i Kupffer cells. These authors demonstrated that was attained, [Ca’+]i decreased to a level which re- prostaglandin D2 increases glycogenolysis in mained elevated above basal values for the dura- hepatocytes and perfused livers and suggested that tion of our experiments (3 min). In 7 individual PAF stimulates glycogenolysis in liver indirectly by cells, exposure to PAF increased [Ca2+]i to stimulation of Kupffer cell prostaglandin D2 pro- 118 + 23 nM (p

25 Volume 25 1, number 1,2 FEBS LETTERS July 1989 studies in perfused livers have suggested the possi- I71 Shukla, S.D., Buxton, D.B., Olson, M.S. and Hanahan, ble involvement of increases in Kupffer cell [Ca2+]i D.J. (1983) J. Biol. Chem. 258, 10212-10214. in the biologic actions of PAF [13]. PI Okayasu, T., Hasegawa, K. and Ishibashi, T. (1987) J. Lipid Res. 28, 760-767. In view of the foregoing evidence, it seems ap- I91 Hill, C.E. and Olson, M.S. (1987) Biochem. J. 247, propriate to suggest that Kupffer cells play a 207-214. primary role in hemodynamic and metabolic re- UOI Hems, D.A. and Whitton, P.D. (1980) Physiol. Rev. 60, sponses to PAF in liver. We propose that PAF l-50. Vll Hill, C.E., Miwa, M., Sheridan, P.J., Hanahan, D.J. and binding to Kupffer cells increases [Ca2+]i resulting Olson, M.S. (1988) Biochem. J. 253, 651-657. in hepatic vasoconstriction by alterations in Kupf- WI McCuskey, R.S. (1966) Am. J. Anat. 119, 455-478. fer cell shape or ‘sphincter cell’ activity. Hydrolysis El31 Lapointe, D.S., Hanahan, D.J. and Olson, M.S. (1987) of inositol lipids by receptor-mediated phospho- 26, 1568-1574. lipase C activation leads to production of second 1141 Berridge, M.J. (1987) Annu. Rev. Biochem. 56, 159-193. [I51 Pilaro, A.M. and Laskin, D.L. (1986) J. Leukocyte Biol. messengers which can increase [Ca2+]i by effects on 40, 29-41. Ca2+ mobilization and/or Ca2+ influx [ 141. While I161 Van Bossuyt, H. and Wisse, E. (1988) J. Hepatol. 7, we have not defined the mechanisms underlying 45-56. PAF-induced increases in [Ca2+]i in Kupffer cells, P71 Brown, E., Kendall, D.A. and Nahorski, S.R. (1984) J. Neurochem. 42, 1379-1387. our results are consistent with a role for inositol [181 Sharma, R.V. and Bhalla, R.C. (1989) Hypertension 13, in lipid biosignalling in this process. press. 1191 Tokumura, A., Homma, H. and Hanahan, D.J. (1985) J. Biol. Chem. 260, 12710-12714. WI Maclntyre, D.E., McNicol, A. and Drummond, A.H. REFERENCES (1985) FEBS Lett. 180, 160-164. WI Homma, H. and Hanahan, D.J. (1988) Arch. Biochem. 111Buxton, D.B., Shukla, SD., Hanahan, D.J. and Olson, Biophys. 262, 32-39. M.S. (1984) J. Biol. Chem. 259, 1468-1471. [221 Handley, D.A., Van Valen, R.G., Winslow, C.M., PI Fisher, R.A., Shukla, S.D., Debuysere, M.S., Hanahan, Tomesch, J.C. and Saunders, R.N. (1987) Thromb. D.J. and Olson, M.S. (1984) J. Biol. Chem. 259, Haemost. 57, 187-190. 8685-8688. 1231 Slivka, S.R. and Insel, P.A. (1988) J. Biol. Chem. 263, [31 Buxton, D.B., Fisher, R.A., Hanahan, D.J. and Olson, 14640-14647. M.S. (1986) J. Biol. Chem. 261, 644-649. 1241 Kuiper, J., Derijke, Y.B., Zijlstra, F.J., Van Waas, M.P. I41 Fisher, R.A., Kumar, R., Hanahan, D.J. and Olson, M.S. and Van Berkel, T.J.C. (1988) Biochem. Biophys. Res. (1986) J. Biol. Chem. 261, 8817-8823. Commun. 157, 1288-1295. [51 Mendlovic, F., Corvera, S. and Garcia-Sainz, J.A. (1984) 1251 Lapointe, D.S. and Olson, MS. (1988) J. Cell Biol. 107, Biochem. Biophys. Res. Commun. 123, 507-514. 858A (Abstr.). 161 Charest, R., Prpic, V., Exton, J.H. and Blackmore, P.F. WI Chao, W., Siafaka-Kapadai, A., Olson, M.S. and (1985) Biochem. J. 227, 79-90. Hanahan, D.J. (1989) Biochem. J. 257, 823-829.

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