DOCSLIB.ORG

Mycoplasma Ellychniae Sp. Nov., a Sterol-Requiring Mollicute from the Firefly Beetle Ellychnia Comma JOSEPH G

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Mycoplasma Ellychniae Sp. Nov., a Sterol-Requiring Mollicute from the Firefly Beetle Ellychnia Comma JOSEPH G INTERNATIONAL JOURNAL OF SYSTEMATICBACTERIOLOGY, July 1989, p. 284-289 Vol. 39, No. 3 0020-7713/89/030284-06$02.00/0 Copyright 0 1989, International Union of Microbiological Societies Mycoplasma ellychniae sp. nov., a Sterol-Requiring Mollicute from the Firefly Beetle Ellychnia comma JOSEPH G. TULLY,l* DAVID L. ROSE,l KEVIN J. HACKETT,2 ROBERT F. WHITCOMB,2 PATRICIA CARLE,' JOSEPH M. BOVE,3 DAVID E. COLFLESH,4 AND DAVID L. WILLIAMSON' Mycoplasma Section, Laboratory of Molecular Microbiology, National Institute of Allergy and Injectious Diseases, Frederick Cancer Research Facility, Frederick, Maryland 21 701 I; Insect Pathology Laboratory, United States Department of Agriculture, Beltsville, Maryland 2070j2; Laboratoire de Biologie Cellulaire et Mole'culaire, Institut Nationale de Recherche Agronomique, Pont-de-la-Maye, France3; and Department of Anatomical Sciences, State University of New York, Stony Brook, New York 1179# Strain ELCN-lT (T = type strain), which was isolated from the hemolymph of the firefly beetle Ellychniu corrusca (Co1eoptera:Lampyridae) in Maryland, was shown to be a sterol-requiring mollicute. Electron and dark-field microscopy showed that the organism consisted of small, nonhelical, nonmotile, pleomorphic coccoid cells. Individual cells were surrounded by a single cytoplasmic membrane, but no evidence of a cell wall was observed. The organism grew well in SP-4 broth medium containing fetal bovine serum, but failed to grow in formulations containing horse serum or bovine serum fraction supplements. Growth on solid media occurred only when agar cultures were incubated aerobically or in an atmosphere containing 5% carbon dioxide. Strain ELCN-lTcatabolized glucose but did not hydrolyze arginine or urea. The optimum temperature for growth was 30"C, while multiplication occurred over a temperature range of 18 to 32°C. Growth was not observed at 37°C. The genome of strain ELCN-lT was determined to be about 585 megadaltons. The guanine-plus-cytosine content of the deoxyribonucleic acid was found to be 27.5 mol%. This organism was serologically unrelated to the type strains of previously described Mycoplusmu species and to 18 other unclassified sterol-requiringisolates cultivated from various animal, plant, or insect sources. This is the first documented insect-derived species in the genus Mycoplasmu. Strain ELCN-1 (= ATCC 43707) is the type strain of Mycoplasma ellychniue sp. nov. The presence of wall-less procaryotes (class Mollicutes) in acholeplasmas (Acholeplasma axanthum, Acholeplasma oc- the microbial flora of insects and other arthropods first uli), while others were subsequently found to represent a became a recognized possibility with the 1967 discovery of new species (e.g., Acholeplasma florum) clearly associated mycoplasma-like organisms in plants infected with the leaf- with and known only from plant hosts (21). The occurrence hopper-borne mulberry dwarf disease agent (12). Although of acholeplasmas in insects was established when Clark and wall-less procaryotes have yet to be cultivated axenically colleagues (6) reported the isolation of sterol-nonrequiring from infected vectors or plants with mulberry disease or mollicutes from the guts of five different insect species. from any of the more than 300 other known mycoplasma-like Some of these isolates, including representatives from a organism-associated diseases, infections caused by myco- deerfly (Chrysops discalis) and a vespid wasp (Monobia plasma-like organisms have been associated consistently quadridens) and strains recovered from other insects (32), with specific insect vectors (22). By the early 1970s, two other important observations provided substantial evidence were identified as A.Jloridm. These observations confirmed a of insect association with mollicutes. Cultivation and char- direct ecological link between acholeplasmas on surfaces of acterization of the first helical mollicute (Spiroplasma citri) plants or flowers and an insect reservoir. More recently, from stubborn-diseased citrus plants (9, 26) had occurred, other acholeplasmas isolated from insect guts have turned and the organism was shown to be transmissible to citrus by out to be new species (e.g., Acholeplasma entomophilum) experimentally infected leafhoppers (11). In addition, the (31) or putative new species (30). then uncultivated corn stunt spiroplasma was shown to be The possibility that sterol-requiring Mycoplasma species transmitted by leafhoppers (see review in reference 34). may, like acholeplasmas, be specifically associated with Although the role of insects as vectors of plant-pathogenic insect-plant interactions has been suspected but not clearly spiroplasmas was established in these studies, it remained established previously (30). A number of putative new for Clark and colleagues to demonstrate that a diversity of species in the genus Mycoplasma have been cultivated from insects harbored a extraordinarly rich spiroplasma flora (5, plant sources and are in various stages of characterization 18). (30). Recently, 30 mollicutes isolated by Clark and col- The association of other mollicutes with insects followed leagues from the guts or hemolymphs of a wide range of the discovery of spiroplasmas in these hosts. The presence insect species were examined (32); 5 of these strains were of nonhelical mollicutes on plant surfaces was first well found to be sterol-requiring, nonhelical mollicutes. In this documented in 1979 (13, 23), when organisms with the paper we describe the detailed taxonomic features of strain general features of both Acholeplasmn and Mycoplasma ELCN-lT (T = type strain), which was isolated from he- species were isolated from vegetative and floral plant parts. molymph of the firefly beetle Ellychnia corrusca, and pro- Some of these isolates were serologically related to known pose that this organism be assigned to the genus Myco- plasma. This is the first species in this genus to be clearly * Corresponding author. shown to be specifically associated with insects. 284 VOL. 39, 1989 MYCOPLASMAELLYCHNIAE SP. NOV. 285 TABLE 1. Mollicute strains and antisera tested TABLE 1-Continued ~ ~~ Strain Source" Strain Source" ~ Mycoplasma agalactiae PG2T .................................. .NIH Mycoplasma pneumoniae FHT .................................. NIH Mycoplasma alkalescens D12T ................................. .MS-LMM Mycoplasma primatum HRC292T.............................. .MS-LMM Mycoplasma alvi IlsleyT .......................................... .MS-LMM Mycoplasma pullorum CKKT ................................... .MS-LMM Mycoplasma anatis 1340T........................................ .NIH Mycoplasma pulmonis PG34T.. ................................. .NIH Mycoplasma anseris 1219T ...................................... .MS-LMM Mycoplasma putrefaciens KS-lT.. ............................. .MS-LMM Mycoplasma arginini G230T..................................... .NIH Mycoplasma salivarium PG20T.. ............................... .NIH Mycoplasma arthritidis PG6T ................................... .NIH Mycoplasma spumans PG13T. ................................... NIH Mycoplasma bovigenitalium PGllT ........................... .NIH Mycoplasma sualvi Mayfield BT ............................... .MS-LMM Mycoplasma buvirhinis PG43T.. ................................ .NIH Mycoplasma subdolum TBT..................................... .MS-LMM Mycoplasma bovis DonettaT .................................... .MS-LMM Mycoplasma synoviae WVU 1853T ........................... .MS-LMM Mycoplasma bovoculi M165/69T ................................MS-LMM Mycoplasma testudinis 01008T.. ................................ .MS-LMM Mycoplasma buccale CH20247T ................................ NIH Mycoplasma verecundum 107T ................................. .MS-LMM Mycoplasma californicum ST-6T................................ MS-LMM Mycoplasma sp. strain California calf .............MS-LMM Mycoplasma canadense 275CT ..................................MS-LMM Mycoplusma sp. strain B5P (bovine joint) ...................MS-LMM Mycoplasma canis PG14 ..........................................NIH Mycoplasma sp. strain melaleuca (plant) .................... .MS-LMM Mycoplasma capricolum California KidT.................... .MS-LMM Mycoplasma sp. strain 831-C4 (lettuce) ...................... .MS-LMM Mycoplasma caviae G122T ...................................... .MS-LMM Mycoplasma sp. strain PPAV (apple) ........................ .MS-LMM Mycoplasma cavipharyngis 117CT............................. .MS-LMM Mycoplasma sp. strain F38 (caprine lung) .................. .MS-LMM Mycoplasma citelli RG-2CT ..................................... .MS-LMM Mycoplusma sp. strain M7806 (human abscess) ............MS-LMM Mycoplasma cloacale 383T ...................................... .MS-LMM Mycoplasma sp. strain 3306 (ovine genitalia) ...............MS-LMM Mycoplasma collis 58BT.......................................... .MS-LMM Mycoplasma sp. strain M2851 (equine uterus) ..............MS-LMM Mycoplasrna colurnbinasale 694T ...............................MS-LMM Mycoplasma sp. strain S5251 (seal lung) .................... .MS-LMM Mycoplasma columbinum MMP-lT .......................... ..MS-LMM Mycoplasma sp. strain HRC291 (primate throat) ..........MS-LMM Mycoplasma columborale MMP-4T ........................... .MS-LMM Mycoplasrna sp. strain 3446 (bovine fetus) ..................MS-LMM Mycoplasma conjunctivae HRC581T.. ........................ .MS-LMM Mycoplasma sp. strain G145 (goat leg). .....................
Load more

Recommended publications
  • The Role of Earthworm Gut-Associated Microorganisms in the Fate of Prions in Soil
    THE ROLE OF EARTHWORM GUT-ASSOCIATED MICROORGANISMS IN THE FATE OF PRIONS IN SOIL Von der Fakultät für Lebenswissenschaften der Technischen Universität Carolo-Wilhelmina zu Braunschweig zur Erlangung des Grades eines Doktors der Naturwissenschaften (Dr. rer. nat.) genehmigte D i s s e r t a t i o n von Taras Jur’evič Nechitaylo aus Krasnodar, Russland 2 Acknowledgement I would like to thank Prof. Dr. Kenneth N. Timmis for his guidance in the work and help. I thank Peter N. Golyshin for patience and strong support on this way. Many thanks to my other colleagues, which also taught me and made the life in the lab and studies easy: Manuel Ferrer, Alex Neef, Angelika Arnscheidt, Olga Golyshina, Tanja Chernikova, Christoph Gertler, Agnes Waliczek, Britta Scheithauer, Julia Sabirova, Oleg Kotsurbenko, and other wonderful labmates. I am also grateful to Michail Yakimov and Vitor Martins dos Santos for useful discussions and suggestions. I am very obliged to my family: my parents and my brother, my parents on low and of course to my wife, which made all of their best to support me. 3 Summary.....................................................………………………………………………... 5 1. Introduction...........................................................................................................……... 7 Prion diseases: early hypotheses...………...………………..........…......…......……….. 7 The basics of the prion concept………………………………………………….……... 8 Putative prion dissemination pathways………………………………………….……... 10 Earthworms: a putative factor of the dissemination of TSE infectivity in soil?.………. 11 Objectives of the study…………………………………………………………………. 16 2. Materials and Methods.............................…......................................................……….. 17 2.1 Sampling and general experimental design..................................................………. 17 2.2 Fluorescence in situ Hybridization (FISH)………..……………………….………. 18 2.2.1 FISH with soil, intestine, and casts samples…………………………….……... 18 Isolation of cells from environmental samples…………………………….……….
    [Show full text]
  • Engineering the Genome of Minimal Bacteria Using CRISPR/Cas9 Tools Iason Tsarmpopoulos
    Engineering the genome of minimal bacteria using CRISPR/Cas9 tools Iason Tsarmpopoulos To cite this version: Iason Tsarmpopoulos. Engineering the genome of minimal bacteria using CRISPR/Cas9 tools. Mi- crobiology and Parasitology. Université de Bordeaux, 2017. English. NNT : 2017BORD0787. tel- 01834971 HAL Id: tel-01834971 https://tel.archives-ouvertes.fr/tel-01834971 Submitted on 11 Jul 2018 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. THÈSE PRÉSENTÉE POUR OBTENIR LE GRADE DE DOCTEUR DE L’UNIVERSITÉ DE BORDEAUX ÉCOLE DOCTORALE Science de la vie et de la Santé SPÉCIALITÉ Microbiologie and Immunologie Par Iason TSARMPOPOULOS Ingénierie de génome de bactéries minimales par des outils CRISPR/Cas9 Sous la direction de : Monsieur Pascal SIRAND-PUGNET Soutenue le jeudi 07 décembre 2017 à 14h00 Lieu : INRA, 71 avenue Edouard Bourlaux 33882 Villenave d'Ornon salle Amphithéâtre Josy et Colette Bové Membres du jury : Mme Cécile BEBEAR Université de Bordeaux et CHU de Bordeaux Président Mme Florence TARDY Anses-Laboratoire de Lyon Rapporteur M. Matthieu JULES Institut Micalis, INRA and AgroParisTech Rapporteur M. David BIKARD Institut Pasteur Examinateur M. Fabien DARFEUILLE INSERM U1212 - CNRS UMR 5320 Invité Mme Carole LARTIGUE-PRAT INRA - Université de Bordeaux Invité M.
    [Show full text]
  • Mycoplasma Orale “Types” 2 and 3, Respectively E
    INTERNATIONAL JOURNAL of SYSTEMATIC BACTERIOLOGY Vol. 24, No. 2 April 1974, p. 252-255 Printed in U.S.A. Copyright 0 1974 International Association of Microbiological Societies Proposal of Mycoplasma buccale nom. nov. and Mycoplasmafaucium nom. nov. for Mycoplasma orale “Types” 2 and 3, Respectively E. A. FREUNDT, D. TAYLOR-ROBINSON, R. H. PURCELL, R. M. CHANOCK, and F. T. BLACK Institute of Medical Microbiology, University of Aarhus, Aarhus, Denmark; MRC Clinical Research Centre, Harrow, Middlesex, England; and Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20014 Following recommendations made by the Subcommittee on the Taxonomy of Mycoplasrnatales of the International Committee on Systematic Bacteriology, it is proposed that Mycoplasma orale 2 and Mycoplasrna orale 3 be recognized as two separate species, Mycoplasrna buccale nom. nov. (type strain: CH20247; ATCC 23636) and Mycoplasrna fauciurn nom. nov. (type strain: DC-333; ATCC 25293), respectively. The general properties and distinctive characteristics of the newly named species are summarized. At present, three “types” of Mycoplasrna (22) be recognized as a species under the new orale are recognized: M. orale 1 Taylor- name Mycoplasrna buccale (L. adj. buccalis Robinson et al. 1964 (21), M. orale 2 Taylor- buccal), and (ii) Mycoplasrna orale 3 Fox et al. Robinson et al. 1965 (22), and M. orale 3 Fox 1969 (7) be recognized as a species under the et al. 1969 (7). However, the authors who new name Mycoplasma fauciurn (L. noun described the latter two “types” or “serotypes” fauces the throat; L. gen. pl. noun fauciurn of did, in fact, regard them as distinct new species throats).
    [Show full text]
  • (12) United States Patent (10) Patent No.: US 8,835,117 B2 Mitchell Et Al
    US008835117B2 (12) United States Patent (10) Patent No.: US 8,835,117 B2 Mitchell et al. (45) Date of Patent: Sep. 16, 2014 (54) NUCLEICACIDS FOR DETECTION AND OTHER PUBLICATIONS DISCRIMINATION OF GENOTYPES OF CHLAMYDOPHILA PSITTAC Nazarenko, I. Methods in Molecular Biology (2006) 335: 95-114.* Jeffrey et al. Microbiology (2007) 153: 2679-2688.* 75) Inventors: Stephaniep L. Mitchell, Somerville, MA Geens et al. Journal of Clinical Microbiology (2005) 43(5): 2456 (US); Jonas M. Winchell, Lilburn, GA 2461. (US) Geens et al., “Development of a Chlamydophila psittaci species specific and genotype-specific real-time PCR.” Vet. Res., 36: 787 (73) Assignee: The United States of America as 797, 2005. represented by the Secretary of the Geens et al., “Sequencing of the Chlamydophila psittaci OmpA Gene Department of Health and Human Reveals a New Genotype, E/B, and the Need for a Rapid Discrimi Services, Centers for Disease Control natory Genotyping Method.” J. Clin. Microbiol. 43(5): 2456-2461, and Prevention, Washington, DC (US) 2005. Heddema, “Genotyping of Chlamydophila psittaci in Human (*) Notice: Subject to any disclaimer, the term of this Samples.” Emerging Infectious Diseases, 12(12): 1989-1990, 2006. patent is extended or adjusted under 35 Menard, “Development of a real-time PCR for the detection of U.S.C. 154(b) by 82 days. Chlamydia psittaci,” J. Med. Microbiol. 55(Pt. 4): 471-473, 2006. Mitchellet al., “Genotyping of Chlamydophilapsittaci by Real-Time (21) Appl. No.: 13/322,787 PCR and High-Resolution Melt Analysis,” Journal of Clinical Microbiology, 47(1): 175-181, 2009. (22) PCT Filed: May 28, 2010 Sachse et al., “Genotyping of Chlamydophila psittaci using a new DNA microarray assay based on sequence analysis of ompA genes.” (86).
    [Show full text]
  • Mycoplasma Pneumoniae Terminal Organelle
    MYCOPLASMA PNEUMONIAE TERMINAL ORGANELLE DEVELOPMENT AND GLIDING MOTILITY by BENJAMIN MICHAEL HASSELBRING (Under the Direction of Duncan Charles Krause) ABSTRACT With a minimal genome containing less than 700 open reading frames and a cell volume < 10% of that of model prokaryotes, Mycoplasma pneumoniae is considered among the smallest and simplest organisms capable of self-replication. And yet, this unique wall-less bacterium exhibits a remarkable level of cellular complexity with a dynamic cytoskeleton and a morphological asymmetry highlighted by a polar, membrane-bound terminal organelle containing an elaborate macromolecular core. The M. pneumoniae terminal organelle functions in distinct, and seemingly disparate cellular processes that include cytadherence, cell division, and presumably gliding motility, as individual cells translocate over surfaces with the cell pole harboring the structure engaged as the leading end. While recent years have witnessed a dramatic increase in the knowledge of protein interactions required for core stability and adhesin trafficking, the mechanism of M. pneumoniae gliding has not been defined nor have interdependencies between the various terminal organelle functions been assessed. The studies presented in the current volume describe the first genetic and molecular investigations into the location, components, architecture, and regulation of the M. pneumoniae gliding machinery. The data indicate that cytadherence and gliding motility are separable properties, and identify a subset of M. pneumoniae proteins contributing directly to the latter process. Characterizations of novel gliding-deficient mutants confirm that the terminal organelle contains the molecular gliding machinery, revealing that with the loss of a single terminal organelle cytoskeletal element, protein P41, terminal organelles detach from the cell body but retain gliding function.
    [Show full text]
  • Coleoptera: Lampyridae)
    Brigham Young University BYU ScholarsArchive Theses and Dissertations 2020-03-23 Advances in the Systematics and Evolutionary Understanding of Fireflies (Coleoptera: Lampyridae) Gavin Jon Martin Brigham Young University Follow this and additional works at: https://scholarsarchive.byu.edu/etd Part of the Life Sciences Commons BYU ScholarsArchive Citation Martin, Gavin Jon, "Advances in the Systematics and Evolutionary Understanding of Fireflies (Coleoptera: Lampyridae)" (2020). Theses and Dissertations. 8895. https://scholarsarchive.byu.edu/etd/8895 This Dissertation is brought to you for free and open access by BYU ScholarsArchive. It has been accepted for inclusion in Theses and Dissertations by an authorized administrator of BYU ScholarsArchive. For more information, please contact [email protected]. Advances in the Systematics and Evolutionary Understanding of Fireflies (Coleoptera: Lampyridae) Gavin Jon Martin A dissertation submitted to the faculty of Brigham Young University in partial fulfillment of the requirements for the degree of Doctor of Philosophy Seth M. Bybee, Chair Marc A. Branham Jamie L. Jensen Kathrin F. Stanger-Hall Michael F. Whiting Department of Biology Brigham Young University Copyright © 2020 Gavin Jon Martin All Rights Reserved ABSTRACT Advances in the Systematics and Evolutionary Understanding of Fireflies (Coleoptera: Lampyridae) Gavin Jon Martin Department of Biology, BYU Doctor of Philosophy Fireflies are a cosmopolitan group of bioluminescent beetles classified in the family Lampyridae. The first catalogue of Lampyridae was published in 1907 and since that time, the classification and systematics of fireflies have been in flux. Several more recent catalogues and classification schemes have been published, but rarely have they taken phylogenetic history into account. Here I infer the first large scale anchored hybrid enrichment phylogeny for the fireflies and use this phylogeny as a backbone to inform classification.
    [Show full text]
  • Chlamydia Trachomatis and Genital Mycoplasmas: Pathogens with an Impact on Human Reproductive Health
    Hindawi Publishing Corporation Journal of Pathogens Volume 2014, Article ID 183167, 15 pages http://dx.doi.org/10.1155/2014/183167 Review Article Chlamydia trachomatis and Genital Mycoplasmas: Pathogens with an Impact on Human Reproductive Health SunIanica Ljubin-Sternak1 and Tomislav MeštroviT2 1 Teaching Institute of Public Health “Dr Andrija Stampar”ˇ and School of Medicine, University of Zagreb, Salataˇ 3b, 10000 Zagreb, Croatia 2Clinical Microbiology and Parasitology Unit, Polyclinic “Dr Zora Profozi´c”, Bosutska 19, 10000 Zagreb, Croatia Correspondence should be addressed to Suncanicaˇ Ljubin-Sternak; [email protected] Received 29 September 2014; Revised 9 December 2014; Accepted 11 December 2014; Published 31 December 2014 Academic Editor: Nongnuch Vanittanakom Copyright © 2014 S. Ljubin-Sternak and T. Meˇstrovic.´ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The most prevalent, curable sexually important diseases are those caused by Chlamydia trachomatis (C. trachomatis) and genital mycoplasmas. An important characteristic of these infections is their ability to cause long-term sequels in upper genital tract, thus potentially affecting the reproductive health in both sexes. Pelvic inflammatory disease (PID), tubal factor infertility (TFI), and ectopic pregnancy (EP) are well documented complications of C. trachomatis infection in women. The role of genital mycoplasmas in development of PID, TFI, and EP requires further evaluation, but growing evidence supports a significant role for these in the pathogenesis of chorioamnionitis, premature membrane rupture, and preterm labor in pregnant woman. Both C. trachomatis and genital mycoplasmas can affect the quality of sperm and possibly influence the fertility of men.
    [Show full text]
  • Mycoplasma Hominis Infection in Spontaneous Abortions in Thrace Population: Detection By
    etics & E en m G b ry n o a l o Iliopoulou et al., Human Genet Embryol 2017, 7:3 m g u y H Human Genetics & Embryology DOI: 10.4172/2161-0436.1000142 ISSN: 2161-0436 Research Article Open Access Mycoplasma hominis Infection in Spontaneous Abortions in Thrace Population: Detection by PCR Iliopoulou S1, Pagonopoulou O2, Tsigalou C3, Deftereou TE1, Koutlaki N4, Tsikouras P4, Papadatou V1, Tologkos S1, Alexiadis T1, Alexopoulou SP1 and Lambropoulou M1* 1Department of Histology-Embryology, Democritus University of Thrace, Alexandroupolis, Greece 2Department of Physiology, Democritus University of Thrace, Alexandroupolis, Greec 3Department of Medical Biopathology, University General Hospital of Alexandroupolis, Greece 4Department of Obstetrics and Gynecology, Democritus University of Thrace, Alexandroupolis, Greece Abstract Objective: The main purpose of this study was the detection of the bacterial strain Mycoplasma hominis, which is one of the 14 Mycoplasma species, in spontaneous abortions. Methods: Placental tissues from 59 miscarriages of the first and second trimester from different areas of Thrace were used. DNA was extracted using a specific kit and the presence of theMycoplasma hominis strain was detected by PCR. Results: Among the samples that were examined 2 were proven to be infected by Mycoplasma hominis. Conclusion: The results of our research imply that a small percentage (~3.6%) of spontaneous abortions could be due to the presence of Mycoplasma hominis. PCR is a reliable, sensitive and easy method for the determination of this type of infection and could be applied in the clinical routine. Keywords: Mycoplasma hominis; PCR; Placenta; Miscarriage It is known that Mycoplasma hominis is associated with harmful effects on women’s reproductive health, as recurrent spontaneous Introduction abortion [10] and with pregnancy complications, as ectopic pregnancy, Mycoplasma hominis is a prokaryotic microorganism with stable preterm birth, preterm prelabour rupture of the membranes (PPROM), morphology.
    [Show full text]
  • Comprehensive Analysis of Risk Factors for Periodontitis Focusing on the Saliva Microbiome and Polymorphism
    International Journal of Environmental Research and Public Health Article Comprehensive Analysis of Risk Factors for Periodontitis Focusing on the Saliva Microbiome and Polymorphism Naoki Toyama 1,* , Daisuke Ekuni 1 , Daisuke Matsui 2, Teruhide Koyama 2 , Masahiro Nakatochi 3, Yukihide Momozawa 4, Michiaki Kubo 4 and Manabu Morita 1 1 Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan; [email protected] (D.E.); [email protected] (M.M.) 2 Department of Epidemiology for Community Health and Medicine, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kamigyo-ku, Kyoto 602-8566, Japan; [email protected] (D.M.); [email protected] (T.K.) 3 Public Health Informatics Unit, Department of Integrated Health Sciences, Nagoya University Graduate School of Medicine, Nagoya 461-8673, Japan; [email protected] 4 Laboratory for Genotyping Development, RIKEN Center for Integrative Medical Sciences, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama City 230-0045, Japan; [email protected] (Y.M.); [email protected] (M.K.) * Correspondence: [email protected]; Tel.: +81-86-235-6712 Abstract: Few studies have exhaustively assessed relationships among polymorphisms, the micro- biome, and periodontitis. The objective of the present study was to assess associations simultaneously among polymorphisms, the microbiome, and periodontitis. We used propensity score matching with a 1:1 ratio to select subjects, and then 22 individuals (mean age ± standard deviation, 60.7 ± 9.9 years) Citation: Toyama, N.; Ekuni, D.; were analyzed.
    [Show full text]
  • Mycoplasma Spermatophilum, a New Species Isolated from Human Spermatozoa and Cervix AURIOL C
    INTERNATIONALJOURNAL OF SYSTEMATICBACTERIOLOGY, Apr. 1991, p. 229-233 Vol. 41, No. 2 0020-7713/91/020229-05$02.oo/o Copyright 0 1991, International Union of Microbiological Societies Mycoplasma spermatophilum, a New Species Isolated from Human Spermatozoa and Cervix AURIOL C. HILL Medical Research Council Toxicology Unit, Woodmansterne Road, Carshalton, Surrey SM5 4EF, United Kingdom A mycoplasma isolated from human spermatozoa and a human cervix was shown to be serologically distinct from 98 previously recognized MycopEasma and AchoZepZasma spp. Six mycoplasma colonies were cloned and examined in detail for morphology, growth, and biochemical characteristics; five of these were from sperm samples and one was from a cervix. These strains were closely related and had the following properties: guanine-plus-cytosine content of 32 mol%, requirement for sterol, and anaerobic growth. Glucose was not metabolized, and arginine and urea were not hydrolyzed. Strain AH159 (= NCTC 11720) is the type strain of a new species, Mycoplasma spermatophilum. Twelve named Mycoplasma and Acholeplasma species colony isolated from each patient was cloned to produce a have been isolated from the respiratory or genital tracts of pure culture; this was done by initially filtering a broth humans (6). Mycoplasma buccale, Mycoplasma faucium, culture through a 220-nm-pore-size membrane filter, cultur- Mycoplasma lipophilum , Mycoplasma orale, Mycoplasma ing the filtrate on solid medium, transferring a single result- pneumoniae, and Mycoplasma salivarium are found almost ing colony to another agar plate, and inoculating the subse- exclusively in respiratory tracts. Mycoplasma fermentans quent growth into broth. This whole procedure was repeated has been found infrequently in urogenital tracts, while My- an additional four times; thus, the organisms were filter coplasma primatum, a species commonly present in nonhu- cloned five times (29).
    [Show full text]
  • APUTS) Reporting Terminology and Codes Microbiology (V1.0
    AUSTRALIAN PATHOLOGY UNITS AND TERMINOLOGY (APUTS) Reporting Terminology and Codes Microbiology (v1.0) 1 12/02/2013 APUTS Report Information Model - Urine Microbiology Page 1 of 1 Specimen Type Specimen Macro Time Glucose Bilirubin Ketones Specific Gravity pH Chemistry Protein Urobilinogen Nitrites Haemoglobin Leucocyte Esterases White blood cell count Red blood cells Cells Epithelial cells Bacteria Microscopy Parasites Microorganisms Yeasts Casts Crystals Other elements Antibacterial Activity No growth Mixed growth Urine MCS No significant growth Klebsiella sp. Bacteria ESBL Klebsiella pneumoniae Identification Virus Fungi Growth of >10^8 org/L 10^7 to 10^8 organism/L of mixed Range or number Colony Count growth of 3 organisms 19090-0 Culture Organism 1 630-4 LOINC >10^8 organisms/L LOINC Significant growth e.g. Ampicillin 18864-9 LOINC Antibiotics Susceptibility Method Released/suppressed None Organism 2 Organism 3 Organism 4 None Consistent with UTI Probable contamination Growth unlikely to be significant Comment Please submit a repeat specimen for testing if clinically indicated Catheter comments Sterile pyuria Notification to infection control and public health departments PUTS Urine Microbiology Information Model v1.mmap - 12/02/2013 - Mindjet 12/02/2013 APUTS Report Terminology and Codes - Microbiology - Urine Page 1 of 3 RCPA Pathology Units and Terminology Standardisation Project - Terminology for Reporting Pathology: Microbiology : Urine Microbiology Report v1 LOINC LOINC LOINC LOINC LOINC LOINC LOINC Urine Microbiology Report
    [Show full text]
  • Microbial Signatures of Oral Dysbiosis, Periodontitis and Edentulism 2 Revealed by Gene Meter Methodology 3 4 M
    bioRxiv preprint doi: https://doi.org/10.1101/070367; this version posted August 19, 2016. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 Microbial Signatures of Oral Dysbiosis, Periodontitis and Edentulism 2 Revealed by Gene Meter Methodology 3 4 M. Colby Hunter1, Alex E. Pozhitkov2, and Peter A. Noble#3 5 6 *Corresponding author, Peter A Noble, Email: [email protected] 7 8 Authors’ affiliations: 9 10 1. Program in Microbiology, Alabama State University, Montgomery, AL 36101 11 2. Department of Oral Health, University of Washington, Box 3574444, Seattle, Washington 12 98195-7444 Ph: 206-409-6664 13 3. Department of Periodontics, University of Washington, Box 3574444, Seattle, Washington 14 98195-7444 Ph: 206-409-6664 15 16 Authors’ emails: 17 18 Hunter: [email protected] 19 Pozhitkov: [email protected] 20 Noble: [email protected] 21 22 bioRxiv preprint doi: https://doi.org/10.1101/070367; this version posted August 19, 2016. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 23 ABSTRACT (N=305 WORDS) 24 25 Conceptual models suggest certain microorganisms (e.g., the red complex) are indicative of a specific 26 disease state (e.g., periodontitis); however, recent studies have questioned the validity of these models. 27 Here, the abundances of 500+ microbial species were determined in 16 patients with clinical signs of 28 one of the following oral conditions: periodontitis, established caries, edentulism, and oral health.
    [Show full text]