United States Patent (19) 11 Patent Number: 5,679,520 Hogan Et Al
US00567952OA United States Patent (19) 11 Patent Number: 5,679,520 Hogan et al. 45 Date of Patent: Oct. 21, 1997 54 NUCLEICACID PROBES AND METHODS 0133671 7/1984 European Pat. Off. FOR DETECTING EUBACTERIA 0155360 9/1985 European Pat. Off.. 0232085 8/1987 European Pat. Off.. (75 Inventors: James John Hogan; Richard Dana 0245129 11/1987 European Pat. Off.. Smith, both of San Diego; Jo Ann s: f E. Ea 3. - Kop, San Marcos; Sherrol Hoffa 8301073 3/1983 sign a. McDonough, San Diego, all of Calif. 8401 174 3/1984 WIPO 8402721 7/1984 WIPO. 73) Assignee: Strobe Incorporated, San Diego, 8803957 6/1988 WIPO. OTHER PUBLICATIONS 21 Appl. No.: 454,080 New England Biolabs Catalog New England Biolabs, Bev 22 Filed: May 30, 1995 erly, Mass., USA (1986/1987), pp. 60-62. Amikam et al., "Mycoplasmas (Mollicutes) Have a Low Related U.S. Application Data Number of rRNA Genes,” Journal of Bacteriology 158:376-378 (1984). 62 E. iii. 200,866, s f 5. E. Amikam et al., "Ribosomal RNA genes in Mycoplasma,” f 1991, State Go of sel. No. Nucleic Acids Research 10:4215-4222 (1982). 295,208, filed as PCT/US87/03009 Nov. 24, 1987, aban doned, which is a continuation-in-part of Ser. No. 83,542, (List continued on next page.) Aug. 7, 1987, abandoned, which is a continuation-in-part of Ser. No. 934,244, Nov. 24, 1986, abandoned. Primary Examiner-Ardin H. Marschel 51 Int. Cl. C12Q 1/68 Attorney, Agent, or Firm-Lyon & Lyon LLP 52 U.S. C. ............................. 435/6; 43.5/5: 435/810; 57 ABSTRACT 436/501; 536/23.1; 536/24.1; 536/24.3; 536/24.31; 536/24.32; 536/24.33; 935/77: Amethod for preparing probes, as well as several probes for 935/78 use in qualitative or quantitative hybridization assays are disclosed.
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