Taiwania 65(2): 249‒252, 2020 DOI: 10.6165/tai.2020.65.249 NOTE Development and characterization of EST-SSR markers in creeping mazus (Mazus miquelii), and cross-amplification in five related species Masaya YAMAMOTO1,*, Daiki TAKAHASHI2, Chih-Chieh YU3, 4, Hiroaki SETOGUCHI2 1. Hyogo University of Teacher Education, 942-1 Shimokume, Kato-city, Hyogo 673-1494, Japan. 2. Graduate School of Human and Environmental Studies, Kyoto University, Yoshida Nihonmatsu, Sakyo-ku, Kyoto 606-8501, Japan. 3. CAS Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla 666303, China. 4. Center of Plant Ecology, Core Botanical Gardens, Chinese Academy of Sciences, Xishuangbanna 666303, China. *Corresponding author’s email:
[email protected]; Tel: +81-(0)795-44-2201 (Manuscript received 5 February 2020; Accepted 12 April 2020; Online published 18 April 2020) ABSTRACT: Simple sequence repeat (SSR) markers were developed from expressed sequence tags (ESTs) for Mazus miquelii (Mazaceae), one of the most widespread species of the genus found in mainland China to Japan and North America, with the goal of elucidating the hidden genetic diversity and pollination ecology of the species. Of the initial 48 EST-SSR markers designed based on transcriptome data, 36 loci were successfully amplified, 16 of which were polymorphic. Polymorphisms for these markers were tested on 72 individuals from three populations in Japan. Two to eleven alleles per locus were detected, and the levels of observed and expected heterozygosity ranged from 0.181 to 0.708 and 0.154 to 0.715, respectively. Most loci were amplified successfully in five related Japanese and Taiwanese species.