PRODUCT OVERVIEW TaqMan® OpenArray® Pharmacogenomics (PGx) Panel

TaqMan® OpenArray® Pharmacogenomics (PGx) Panel Genotyping analysis of drug metabolism enzymes and associated transport proteins

The TaqMan® OpenArray® Trusted TaqMan® performance samples, studies, and labs. Assays Pharmacogenomics (PGx) Panel is Each TaqMan® DME Genotyping were selected for optimal relevance a powerful tool to help researchers Assay contains two allele-specific to current pharmacogenomics study genetic variation probes and a primer pair to detect studies and organized for a simplified in relation to drug action and its the specific SNP target. Both the workflow. potential application to medical probes and primers uniquely align ® ® treatment. within the genome, enabling the The TaqMan OpenArray PGx TaqMan® genotyping technology to Panel provides valuable data for ® ® The TaqMan OpenArray PGx provide superior specificity. It is this the study of drug interactions in Panel was developed for quick and specificity that allows these assays several research areas. Targeted easy screening of known high- to detect targets residing in highly relate to areas of study such value target genes associated with homologous families that may as cardiovascular (CYP2D6, CYP2C19, drug metabolism enzymes and include pseudogenes. TaqMan® Drug NAT1, NAT2), analgesics (CYP2C9, associated transport proteins. Metabolism Enzyme Genotyping CYP2D6), rheumatology (CYP2C9, The panel consists of 158 drug Assays were developed using a high TPMT), neurology (CYP2C19, CYP2D6), metabolism enzyme (DME) assays level of bioinformatics and wet-lab and musculoskeletal (CYP2C19). A derived from the PharmaADME stringency. All assays have passed total of 29 genes are covered across Core Marker Set (Table 1). The performance tests involving 180 158 unique assays. ® OpenArray plate format allows for unique DNA samples from four high-throughput genotyping with To review a gene list for the PGx different populations. ™ a workflow that is cost-effective panel run on the QuantStudio 12K and easy to use. As a result, the Coverage of key genes for Flex system, go to www.lifetech.com/ TaqMan® OpenArray® PGx Panel pharmacogenomics research studies quantstudiopgxpanel. ® ® is a highly effective solution for The TaqMan OpenArray PGx Panel To review a gene list for the PGx pharmacogenomics applications. is preformatted, cost-effective, panel on the OpenArray Real-Time and easy to use. Results are PCR System, go to www.lifetech. reproducible and consistent across com/pgxpanel.

Table 1. List of gene targets in the TaqMan® OpenArray® Pharmacogenomics (PGx) Panel. PharmaADME Number Class* of genes Human gene symbols Phase I 12 CYP1A1, CYP1A2, CYP2A6, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP2E1, CYP3A4, CYP3A5, DPYD

Phase II 6 GSTP1, NAT1, NAT2, UGT1A1, UGT2B7, UGT2B15 Transporter 11 ABCB1, ABCC2, ABCG2, SLC15A2, SLC22A1, SLC22A2, SLC22A6, SLCO1B1, SLCO1B3, SLCO2B1, TPMT

*Source: www.pharmaadme.org Over 10,000 data points in a single run These panels combine the efficiency of the OpenArray® high-throughput platform with the robust genotyping performance of TaqMan® Assays. Each TaqMan® OpenArray® PGx Panel is configured to run 16 samples per OpenArray® plate. Samples are organized into each array as seen in Figure 1. The QuantStudio™ 12K Flex Real-Time PCR System can run four plates at once, allowing for 64 samples and generating over 10,000 data points in a single run. This allows high-throughput profiling while saving precious sample and lowering reagent costs.

Figure 2. SNP genotyping plot for CYP2C19*2 obtained using the QuantStudio™ 12K Flex System with OpenArray® Block.

Figure 1. TaqMan® OpenArray® PGx Panel sample arrangement. A total of 16 samples can be run on each TaqMan® Open Array® PGx Panel. For each sample, 158 wells are used. The TaqMan® OpenArray® PGx Panel is available for both the QuantStudio™ 12K Flex Real-Time PCR System and the OpenArray® Real-Time PCR System. Users can be confident of high-quality results on any OpenArray® platform. Figures 2 and 3 depict the breadth of data that can be generated using the TaqMan® OpenArray® PGx Panel with the QuantStudio™

12K Flex system. Figure 3. SNP genotyping plot obtained for CYP2D6*4 using the QuantStudio™ 12K Flex System with OpenArray® Block.

Ordering information Product Quantity Cat. No. TaqMan® OpenArray® PGx Panel (for use with the OpenArray® Real-Time PCR System) 1 plate 4475375 TaqMan® OpenArray® PGx Panel (for use with the QuantStudio™ 12K Flex system) 1 plate 4475395

Related products required for use with OpenArray® panels Product Quantity Cat. No. TaqMan® OpenArray® Genotyping Accessories Kit (for use with the OpenArray® Real-Time PCR System) Enough for 10 plates 4404572 QuantStudio™ 12K Flex OpenArray® Accessories Kit Enough for 10 plates 4469576

Contact your local Life Technologies support representative for more information on the panel as well as sample preparation details.

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For Research Use Only. Not for use in diagnostic procedures. ©2012 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation and/or its affiliate(s) or their respective owners. TaqMan and AmpliTaq Gold are registered trademarks of Roche Molecular Systems, Inc., used under permission and license. CO27396 1112