Lungkine (CXCL15), mouse Recombinant Mouse Lungkine (CXCL15)

Instruction Manual

Catalog Number D-66525 Synonyms C-X-C motif 15, Lungkine, Small-inducible B15, Cxcl15, Scyb15 Description Lungkine is a CXC chemokine that is expressed in epithelial cells and, to a lesser extent, in certain fetal tissues. No human homolog has been identified and a specific cell surface receptor has not yet been found. Lungkine expression in lung tissue is elevated in response to inflammation, at which time it acts to specifically recruit and direct them into the lung airway. Recombinant murine Lungkine is a 16.4 kDa non-glycosylated, single polypeptide chain consisting of 142 amino acid residues, including four conserved cysteine residues present in CXC . The rMu CXCL15 has been purified by proprietary chromatographic techniques. Quantity 20 µg Molecular Mass 16.4 kDa Source E. coli Biological-Activity Determined by its ability to chemoattract human neutrophils at concentration range of 20-100 ng/ml using a chemotaxis bioassay. Specific Activity see below Formulation White, lyophilized (freeze-dried) powder. Lyophilized from a (0.2 µm) sterile-filtered, concentrated solution in PBS (pH 7.4) and 0.02% Tween-20. Reconstitution Please Note: Always centrifuge product before opening vial. The lyophilized protein should be reconstituted with sterile, ultrapure water to a concentration of 0.1 - 1.0 mg/ml. For further dilution, carrier protein (0.1% BSA or HSA) should be added to avoid loss of bioactivity. Purity Greater than 95% (determined by SDS-PAGE and RP-HPLC analysis) Endotoxin Level < 0.1 ng per µg of rMu Lungkine (1EU/µg). Storage The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% endotoxin-free HSA or BSA; e.g. Cat.No. C-69500A). Avoid repeated freeze-thaw cycles.

05/2019