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Bacterial Hosts for Production of Bioactive Phenolics from Berry Fruits

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Bacterial Hosts for Production of Bioactive Phenolics from Berry Fruits Downloaded from orbit.dtu.dk on: Sep 24, 2021 BacHBerry:: BACterial Hosts for production of Bioactive phenolics from bERRY fruits Dudnik, Alexey; Almeida, A. Filipa; Andrade, Ricardo; Avila, Barbara; Bañados, Pilar; Barbay, Diane; Bassard, Jean Etienne; Benkoulouche, Mounir; Bott, Michael; Braga, Adelaide Total number of authors: 83 Published in: Phytochemistry Reviews Link to article, DOI: 10.1007/s11101-017-9532-2 Publication date: 2018 Document Version Peer reviewed version Link back to DTU Orbit Citation (APA): Dudnik, A., Almeida, A. F., Andrade, R., Avila, B., Bañados, P., Barbay, D., Bassard, J. E., Benkoulouche, M., Bott, M., Braga, A., Breitel, D., Brennan, R., Bulteau, L., Chanforan, C., Costa, I., Costa, R. S., Doostmohammadi, M., Faria, N., Feng, C., ... Forster, J. (2018). BacHBerry:: BACterial Hosts for production of Bioactive phenolics from bERRY fruits. Phytochemistry Reviews, 17(2), 291-326. https://doi.org/10.1007/s11101-017-9532-2 General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. Users may download and print one copy of any publication from the public portal for the purpose of private study or research. You may not further distribute the material or use it for any profit-making activity or commercial gain You may freely distribute the URL identifying the publication in the public portal If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. 1 TITLE 2 BacHBerry: BACterial Hosts for production of Bioactive phenolics from bERRY fruits 3 RUNNING TITLE 4 BacHBerry 5 6 AUTHORS 7 The list of contributing authors is given on p. 2. 8 9 *Corresponding author: 10 Alexey Dudnik 11 Email: [email protected] 12 Phone: + 45 93 51 11 01 13 Fax: +45 45 25 80 01 14 15 16 Keywords: berries; bioprospecting; microbial cell factories; polyphenols; sustainable production. 17 18 Footnote 19 This article is written by the BacHBerry consortium (www.bachberry.eu) and represents the collective effort 20 of all participating institutions. The authors are therefore listed in alphabetical order. 1 21 The list of authors 22 Alexey Dudnik1,*, A. Filipa Almeida15,20, Ricardo Andrade2, Barbara Avila3, Pilar Bañados3, Diane Barbay4, 23 Jean-Etienne Bassard5, Mounir Benkoulouche4, Michael Bott6, Adelaide Braga7, 21, Dario Breitel12, Rex 24 Brennan10, Laurent Bulteau2, Celine Chanforan8, Inês Costa15,20, Rafael S. Costa9, Mahdi 25 Doostmohammadi9,22, Nuno Faria7, 21, Chengyong Feng17, Armando Fernandes9, Patricia Ferreira7, 21, Roberto 26 Ferro1, Alexandre Foito10, Sabine Freitag10, Gonçalo Garcia15,20, Paula Gaspar1, Joana Godinho-Pereira15,20, 27 Björn Hamberger5, András Hartmann9, Harald Heider4, Carolina Jardim15,20, Alice Julien-Laferriere2, Nicolai 28 Kallscheuer6, Wolfgang Kerbe13, Oscar P. Kuipers11, Shanshan Li17, Nicola Love12, Alberto Marchetti- 29 Spaccamela2, Jan Marienhagen6, Cathie Martin12, Arnaud Mary2, Vincent Mazurek8, Camillo Meinhart13, 30 David Méndez Sevillano14, Regina Menezes15,20, Michael Naesby4, Morten H.H. Nørholm1, Finn T. Okkels8, 31 Joana Oliveira7, 21, Marcel Ottens14, Delphine Parrot2, Lei Pei13, Isabel Rocha7, 21, Rita Rosado-Ramos15,20, 32 Caroline Rousseau4, Marie-France Sagot2, Claudia Nunes dos Santos15,20, Markus Schmidt13, Tatiana 33 Shelenga16, Louise Shepherd10, Ana Rita Silva7, 21, Marcelo Henriques da Silva14, Olivier Simon8, Steen 34 Gustav Stahlhut1, Ana Solopova11, Artem Sorokin16, Derek Stewart10,19, Leen Stougie2, Shang Su17, Vera 35 Thole12, Olga Tikhonova16, Martin Trick12, Philippe Vain12, André Veríssimo9, Ana Vila-Santa9, Susana 36 Vinga9, Michael Vogt6, Liangsheng Wang17, Lijin Wang17, Wei Wei17, Sandra Youssef13, Ana Rute Neves18 37 & Jochen Forster1 38 39 1 The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, 40 Building 220, 2800 Kgs. Lyngby, Denmark 41 2 Institut National de Recherche en Informatique et Automatique, 43 Boulevard du 11 Novembre 1918, 42 69622 Villeurbanne Cedex, France 43 3 Facultad De Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Av. Vicuña 44 Mackenna Ote, 4860 Macul, Chile 45 4 Evolva, Duggingerstrasse 23, 4053 Reinach, Switzerland 46 5 Department of Plant and Environmental Science, University of Copenhagen, 1871 Frederiksberg, Denmark 2 47 6 Forschungszentrum Jülich GmbH, Institut für Bio- und Geowissenschaften, IBG-1: Biotechnologie, 48 Wilhelm-Johnen-Straße, 52428 Jülich, Germany 49 7 Biotempo, Avepark – Edif. Spinpark, Zona Industrial da Gandra, Barco, 4805-017 Guimarães, Portugal 50 8 Chr. Hansen Natural Colors A/S, Agern Alle 24, 2970 Hørsholm, Denmark 51 9 IDMEC, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais 1, 1049-001 Lisboa, 52 Portugal 53 10 The James Hutton Institute, Invergowrie, DD2 5DA Dundee, Scotland, United Kingdom 54 11 Groningen Biomolecular Sciences and Biotechnology Institute, Department of Molecular Genetics, 55 University of Groningen, Linnaeusborg, Nijenborgh 7, 9747 AG Groningen, The Netherlands 56 12 John Innes Centre, Norwich Research Park, NR4 7UH Norwich, United Kingdom 57 13 Biofaction KG, Kundmanngasse 39/12, 1030 Vienna, Austria 58 14 Department of Biotechnology, Delft University of Technology, van der Maasweg 9, 2629 HZ Delft, The 59 Netherlands 60 15 Instituto de Biologia Experimental e Tecnológica, Av. República, Qta. do Marquês, 2780-157 Oeiras, 61 Portugal 62 16 Fruit Crops Genetic Resources Department, N. I. Vavilov Research Institute of Plant Industry, B. 63 Morskaya Street 42-44, 190000 St. Petersburg, Russia 64 17 Institute of Botany, The Chinese Academy of Sciences, 20 Nanxincun, Xiangshan, 100093 Beijing, China 65 18 Chr. Hansen Holding A/S, Bøge Alle 10-12, 2970 Hørsholm, Denmark 66 19Institute of Mechanical, Process and Energy Engineering, School of Engineering and Physical Sciences, 67 Heriot Watt University, Edinburgh, Scotland, UK 68 20Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da 69 República, 2780-157 Oeiras, Portugal. 70 21Centre of Biological Engineering, University of Minho, Campus de Gualtar 4710-057, Braga, Portugal 71 22School of Mathematics, University of Edinburgh, EH9 3FD Edinburgh, Scotland, United Kingdom 3 72 Abstract 73 BACterial Hosts for production of Bioactive phenolics from bERRY fruits (BacHBerry) was a three-year 74 project funded by the Seventh Framework Programme (FP7) of the European Union that ran between 75 November 2013 and October 2016. The overall aim of the project was to establish a sustainable and 76 economically-feasible strategy for the production of novel high-value phenolic compounds isolated from 77 berry fruits using bacterial platforms. The project aimed at covering all stages of the discovery and pre- 78 commercialization process, including berry collection, screening and characterization of their bioactive 79 components, identification and functional characterization of the corresponding biosynthetic pathways, and 80 construction of Gram-positive bacterial cell factories producing phenolic compounds. Further activities 81 included optimization of polyphenol extraction methods from bacterial cultures, scale-up of production by 82 fermentation up to pilot scale, as well as societal and economic analyses of the processes. This review article 83 summarizes some of the key findings obtained throughout the duration of the project. 84 4 85 Introduction 86 BacHBerry (BACterial Hosts for production of Bioactive phenolics from bERRY fruits, www.bachberry.eu) 87 has received funding from the European Commission’s Seventh Framework Programme (FP7) under the 88 theme KBBE.2013.3.1-01: “Plant High Value Products - from discovery to final product” with the goal of 89 bringing together experts from the fields of plant biology, industrial biotechnology, analytical chemistry, and 90 social sciences in order to assemble and validate a complete pipeline for discovery and microbial production 91 of novel phenolic compounds of plant origin. The consortium consisted of twelve academic groups, five 92 small and medium sized enterprises and one large enterprise from eleven different countries, namely Austria, 93 Chile, China, Denmark, France, Germany, The Netherlands, Portugal, Russia, Switzerland and the United 94 Kingdom. The diversity of partners has allowed access to a variety of expertise and to plant material that had 95 not been characterized extensively. 96 Polyphenols as a source of bioactive molecules 97 Historically, plants have been not only important sources of nutrients and energy in the human diet but also 98 have provided the fundamentals of traditional and modern medicine. The Plantae kingdom has been 99 estimated to produce more than 200,000 different chemical compounds (Weckwerth 2003), including 100 compounds with proven and potential medical applications. This is reflected in the high number of 101 pharmaceutical products based on, or derived from, plant natural products, such as aspirin, morphine, 102 quinine, paclitaxel and artemisinin (Cragg and Newman 2013). Among the different classes of specialized 103 (i.e. secondary) metabolites phenylpropanoids stand out due to their diversity and ubiquity in the plant 104 kingdom. Fleshy fruits, a rich source of bioactive components,
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