SEARCHING FOR ANTICANCER AGENTS AND ANTIMALARIAL AGENTS FROM MADAGASCAR
Ende Pan
A thesis submitted to the faculty of the Virginia Polytechnic Institute and State University in partial fulfillment of the requirements for the degree of
Doctor of Philosophy In Chemistry
Dr. David G. I. Kingston Dr. Paul R. Carlier Dr. Harry W. Gibson Dr. Brian E. Hanson Dr. James M. Tanko
(December 8, 2010) Blacksburg, VA
Keywords: Natural Products, Anticancer, Antimalarial, Antiproliferative, Flavonoid, Cardenolide, Diphenylpropane, Alkaloid, Sesquiterpene lactone
SEARCHING FOR ANTICANCER AGENTS AND ANTIMALARIAL AGENTS FROM MADAGASCAR
Ende Pan
ABSTRACT
In our continuing search for biologically active natural products from Madagascar as part of an International Cooperative Biodiversity Group (ICBG) program, a total of four antiproliferative extracts were studied, leading to the isolation of twelve novel compounds with antiproliferative activity against the A2780 human ovarian cancer line, and one extract with antimalarial activities was studied, which led to the isolation of five new natural products with antimalarial activities against the Dd2 and HB3 malarial parasites.
The plants and their metabolites are discussed in the following order: one new xanthone and two known guttiferones from Symphonia tanalensis Jum. & H. Perrier
(Clusiaceae); four new diphenyl propanes and one new cyclohepta-dibenzofuran skeleton from Bussea sakalava (Fabaceae); four new cardiac glycosides from Leptadenia madagascariensis Decne. (Apocynaceae); two new and four known alkaloids from
Ambavia gerrardii (Baill.) Le Thomas (Annonaceae); five new sesquiterpene lactones from Polycline proteiformis Humbert (Asteraceae).
The structures of all compounds were determined by analysis of their mass spectrometric, 1D and 2D NMR, UV and IR spectroscopic and optical rotation data.
Other than structure elucidation, this dissertation also involve bioactivity evaluation of all the isolates, synthesis of two interesting alkaloids, as well as a proposal for the possible biosynthetic pathway of the new cyclohepta-dibenzofuran skeleton.
ACKNOWLEDGEMENTS
Firstly, I would like to thank my advisor, Dr. David G. I. Kingston, for giving me the opportunity to work in his lab at Virginia Tech. I feel grateful for his guidance, patience, concern and kindness throughout my time in Blacksburg. And I would also like to thank the other members of my committee, Drs. Paul R. Carlier, Harry W. Gibson, Brian E.
Hanson, James M. Tanko, and the former member, Dr. Larry T. Taylor. Their wisdom, advice and encouragement has been greatly appreciated. Special thanks are given to Drs.
Shugeng Cao and Liva Harinantenaina for teaching me so much about natural product chemistry and for providing me invaluable suggestions on research problems. I would like to thank Drs. Paul Roepe and John Alumna at Georgetown University for our collaborative effort on the antimalarial project.
Without the help and support from current and some of the former members of Dr.
Kingston’s group, the current work would not have been possible. So I would like to thank Ms. Peggy Brodie, Drs. Qiao Hong Chen, Yanpeng Hou, Brian Murphy, Vincent E.
Rasamison, Patricia Onocha, Chao Yang, and Jun Qi, Mr. Jielu Zhao, Ms. Yixi Liu, Mr.
Alex Xu, Mr. Yumin Dai and Mrs. Melody Windsor.
I would also like to thank the current and former departmental analytical service staff
Dr. Hugo Azurmendi, Dr. Mehdi Ashraf-Khorassani, Mr. William Bebout, Mr. Tom
Glass, Mr. Geno Iannaccone, and Dr. Carla Slebodnick for their assistance.
Finally, I could not come so far without love, support and encouragement from my family. I would like to thank my parents, Zexin Pan and Yun Sun for always loving me and supporting me so much over the years. I would like to thank my wife, Xian Wang, who has given up so much and always been there for me all these years.
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TABLE OF CONTENT
Page
LIST OF FIGURES ix
LIST OF SCHEMES xi
LIST OF TABLES xii
I. Introduction: natural product drug discovery 1
1.1 Introduction 1
1.2 Natural product drug discovery 2
1.3 Natural products medicines from different sources 4
1.4 Anticancer agents and antimalarial agents from natural resources 7
1.5 The ICBG (International Cooperative Biodiversity Groups) program 10
References 12
II. An antiproliferative xanthone and two guttiferones of Symphonia tanalensis from
the Madagascar rainforest 16
2.1 Introduction 16
2.1.1 Previous investigations of Symphonia 17
2.2.Results and Discussion 18
2.2.1 Structure elucidation of Compound 2.1 18
2.2.2 Identification of the know guttiferone I and A 21
2.2.3 Antiproliferative activities of isolated compounds 22
2.3 Experimental Section 23
iv
References 29
III. Four diphenylpropanes and a cycloheptadibenzofuran from Bussea sakalava from
the Madagascar dry forest 33
3.1 Introduction 33
3.1.1 Previous investigations of Bussea 34
3.2 Results and Discussion 34
3.2.1 Structure elucidation of bussealin A (3.1) 34
3.2.2 Structure elucidation of bussealin B (3.2) 36
3.2.3 Structure elucidation of bussealin C (3.3) 37
3.2.4 Structure elucidation of bussealin D (3.4) 38
3.2.5 Structure elucidation of bussealin E (3.5) 39
3.2.6 Possible biosynthesis of bussealin E (3.5) 42
3.2.7 Antiproliferative activities of bussealin A-E (3.1-3.5) 43
3.3 Experimental Section 44
References 48
IV. Cardenolides of Leptadenia madagascariensis from the Madagascar dry forest 52
4.1 Introduction 52
4.1.1 Previous investigations of Leptadenia 53
4.2 Results and Discussion 54
4.2.1 Structure elucidation of madagascarensilide A (4.1) 54
4.2.2 Structure elucidation of madagascarensilide B (4.2) 58
v
4.2.3 Structure elucidation of madagascarensilide C (4.3) 59
4.2.4 Structure elucidation of madagascarensilide D (4.4) 63
4.2.5 Antiproliferative activities of madagascarensilide A-D (4.1-4.4) 64
4.3 Experimental Section 65
References 69
V. Isolation and synthesis of antiproliferative eupolauridine alkaloids of
Ambavia gerrardii from the Madagascar dry forest 73
5.1 Introduction 73
5.1.1 Previous investigations of Annonaceae 74
5.2 Results and Discussion 75
5.2.1 Structure elucidation of compound 5.1 76
5.2.2 Structure elucidation of compound 5.2 77
5.2.3 Synthesis of compounds 5.1 and 5.2 77
5.2.4 Antiproliferative activities of compounds 5.1-5.6, 5.10 and 5.11 81
5.3 Experimental Section 82
References 90
VI. Five new antimalarial pseudoguaianolides of Polycline proteiformis from the
Madagascar dry forest 95
6.1 Introduction 95
6.1.1 Previous investigations of Asteraceae 95
6.2 Results and Discussion 96
vi
6.2.1 Structure elucidation of polyclinolide A (6.1) 97
6.2.2 Structure elucidation of polyclinolide B (6.2) 100
6.2.3 Structure elucidation of polyclinolide C (6.3) 101
6.2.4 Structure elucidation of polyclinolide D (6.4) 104
6.2.5 Structure elucidation of polyclinolide E (6.5) 105
6.2.6 Structure elucidation of compound 6.6 106
6.2.7 Bioactivities of polyclinolide A-E (6.1-6.5) and centaureidin (6.6) 106
6.3 Experimental Section 109
References 114
VII. Miscellaneous Plants Studied 118
7.1 Introduction 118
7.2 Anticancer extracts 118
7.2.1 Chadsia racemosa (Fabaceae) 118
7.2.2 Gastonia duplicate (Araliaceae) 119
7.2.3 Entada louvelii (Fabaceae) 119
7.2.4 Entada sp. (Fabaceae) 120
7.2.5 Leea guineensis (Vitaceae) 120
7.2.6 Droceloncia reticulate (Euphorbiaceae) 120
7.3 Antimalarial extracts 121
7.3.1 Phyllanthus muellerianus (Euphorbiaceae) 121
7.3.2 Microdemis Caseariaefoli (Pandaceae) 122
7.3.3 Majidea sp. (Sapindaceae) 123
vii
7.3.4 Terminalia septentrionalis (Combretaceae) 124
7.4 Structure elucidation on compounds isolated in Madagascar 124
References 125
VIII. General Conclusions 127
8.1 Anticancer extracts 127
8.2 Antimalarial extracts 128
APPENDIX 129
viii
LIST OF FIGURES
Figure 1.1 Chemical structures of 1.1-1.5 2
Figure 1.2 Chemical structures of 1.6-1.8 2
Figure 1.3 All new chemical entities, 01/1981-06/2006, by source (N ) 1184 3
Figure 1.4 Chemical structures of 1.9-1.12 4
Figure 1.5 Chemical structures of 1.13-1.15 5
Figure 1.6 Chemical structures of 1.16-1.18 6
Figure 1.7 Chemical structures of 1.19 and 1.20 6
Figure 1.8 Chemical structures of 1.21 and 1.22 7
Figure 1.9 Chemical structures of 1.23 and 1.24 8
Figure 1.10 Chemical structures of 1.25 and 1.26 8
Figure 1.11 Chemical structures of 1.27 and 1.28 9
Figure 1.12 Chemical structures of 1.29-1.31 10
Figure 2.1 Compounds isolated from Symphonia globulifera 17
Figure 2.2 Chemical structure of compound 2.1 20
Figure 2.3 NMR correlations of compound 2.1 21
Figure 2.4 Chemical structures of compound 2.2 and2.3 22
Figure 2.5 Chemical structures of compounds 2.4-2.6 22
Figure 3.1 Compounds isolated from Bussea sp. 34
Figure 3.2 Chemical structures of bussealin A-D (3.1-3.4) 36
Figure 3.3 Chemical structures of bussealin E (3.5) and 3.6 40
Figure 3.4 COSY, HMBC and NOESY correlations of 3.5 40
ix
Figure 4.1 Compounds from the genus Leptadenia 53
Figure 4.2 Chemical structures of madagascarensilides A (4.1) and B (4.2) 55
Figure 4.3 a) Key COSY (bold) and HMBC (arrows) correlations for 4.1
b) Key ROESY correlations for 4.1 57
Figure 4.4 Chemical structures of madagascarensilides C (4.3) and D (4.4) 63
Figure 4.5 a) Key COSY (bold) and HMBC (arrows) correlations for 4.3
b) Key ROESY correlations for 4.3 64
Figure 5.1. Compounds isolated from the family Annonaceae 74
Figure 5.2. Chemical structures of compounds 5.1-5.6 75
Figure 5.3. Contributing resonance structures (with positive charge at position 7 or 10) of
5.11, 5.2 80
Figure 6.1 Compounds isolated from the family Asteraceae 96
Figure 6.2 Chemical structures of polyclinolides A-E (6.1-6.5) and centaureidin (6.6) 97
Figure 6.3 a) Key COSY(bold) and HMBC (arrows) correlations of 6.1
b) Key NOESY correlations for 6.1 99
Figure 6.4 Anisotropic displacement ellipsoid drawing of 6.1 100
Figure 6.5 Chemical structures of 6.2 and 6.7 101
Figure 6.6 a) Key COSY (bold) and HMBC (arrows) correlations for 6.3
b) Key NOESY correlations for 6.3
c) Chemical structure of compound 6.8 103
Figure 6.7 COSY (bold) and HMBC (arrows) correlations for 3-hydroxy-3-methyl
pentanoate group at C-8 of 6.2 105
LIST OF SCHEMES
x
Scheme 2.1 96-well plate of A2780 cell line 24
Scheme 2.2 Separation of ethanol extract of Symphonia tanalensis 26
Scheme 3.1 Possible biosynthesis of cycloheptadibenzofuran 3.5 in B. sakalava 43
Scheme 3.2 Separation of ethanol extract of Bussea sakalava 43
Scheme 3.3 HPLC Chromatogram of fraction II 47
Scheme 4.1 Separation of ethanol extract of Leptadenia madagascariensis 67
Scheme 5.1 The synthesis of compound 5.1 and 5.2 78
Scheme 5.2 Separation of ethanol extract of Ambavia gerradii 85
Scheme 6.1 Separation of ethanol extract of Polycline proteiformis 110
LIST OF TABLES
xi
1 13 Table 2.1 H and C NMR Data in CD3OD for Compounds 2.1 19
1 13 Table 3.1 H and C NMR Data for bussealin A-D (3.1-3.4) in CD3OD 39
Table 3.2 1H and 13C NMR Data for bussealin E (3.5) 42
Table 4.1 1H and 13C NMR Data for madagascarensilides A-D (4.1-4.4) 61
Table 5.1 1H and 13C NMR Data for 5.1, 5.2, 5.10 and 5.11 81
Table 5.2 IC50 values of 5.1-5.6, 5.10 and 5.11 against A2780 and H460 cancer cell lines
82
Table 6.1 1H and 13C NMR Data for polyclinilide A-E (6.1-6.5) 107
xii
I. Introduction: natural product drug discovery
1.1 Introduction
The term “ Natural product ” is used commonly in reference to a chemical substance produced by a living organism. Natural products often have pharmacological or biological activities that are useful for pharmaceutical drug discovery and drug design.
The use of natural resources as medicines for human health can be traced back thousands of years, and the use of plants for treating different diseases has been recorded in the medical books of ancient China, India, and North Africa.1 Many of the aqueous, ethanolic, distilled, condensed, or dried plant extracts used had some beneficial medicinal properties for patients. Today, 80% of the world’s population still relies on traditional medicines.2
Beginning in the 19th century, man started to isolate the active components from medicinal plants. The first landmark was made by the French scientists Caventou and
Pelletier, who isolated quinine (1.1) from Cinchona bark.3 This discovery sparked a vast interest in searching for active compounds from plants. Before the 1930s, a series of natural products, such as morphine (1.2), codeine (1.3), digoxin (1.4) and atropine (1.5) were isolated from higher plants, and these compounds continue in clinical use today.1
1
Figure 1.1 Chemical structures of 1.1-1.5
Another milestone was made when the penicillins (1.6) were isolated from a species of Penicillium and used for treating serious infections during World War II.4 In recent years, the isolation of paclitaxel (1.7) in 1967 from the bark of the Western Pacific Yew
Taxus brevifolia and its use for treating cancers,5 and the isolation of artemisinin (1.8) from the Chinese herb Artemisia annua in 1972 and its use for treating malaria6 has increased the attention paid to the field of natural product drug discovery.
Figure 1.2 Chemical structures of 1.6-1.8
1.2 Natural product drug discovery
Natural products provide one of most important sources for novel drug discovery.
Many natural product and natural product derived compounds are being evaluated in clinical trials or in registration,7, 8 and according to a recent report, 28% of new approved drugs were natural product or products derived (Figure 1.1) from natural products among
2 the overall 1184 new chemical entities (NCE) covering all diseases/countries/sources in the years 01/1981-06/2006. 9
Figure 1.3 All new chemical entities, 01/1981-06/2006, by source (N ) 1184.9
B: Biological; usually a large (>45 residues) peptide or protein either isolated from an organism/cell line or produced by biotechnological means in a surrogate host. N: Natural product. ND: Derived from a natural product and is usually a semisynthetic modification. S: Totally synthetic drug, often found by random screening /modification of an existing agent. S*: Made by total synthesis, but the pharmacophore is/was from a natural product. V: Vaccine. Subcategory: NM: Natural product mimic
Of 974 small molecules from the above 1184 NCEs, 34% were natural products or natural product derived. In the area of cancer, from the late 1940s to 06/2006 for 155 approved anticancer small molecule drugs, 73% are other than synthetic products, with
47% actually being either natural products or their derivatives. The antiinfective area
(antibacterial, antifungal, antiparasitic, and antiviral) has been largely dependent on the structures of natural product, and 86 of total 180 small molecule drugs approved from
01/1981 to 06/2006 were natural products or derived therefrom.9 The influence of natural product structures are also quite significant in other areas. Searching for lead natural products as sources of novel structures is still one of the most important approaches for drug discovery.
1.3 Natural product medicines from different sources
3 Today, natural products are obtained not only from terrestrial plants but also from marine organisms, microorganisms and animals. Below are a few examples of natural product medicines from different categories.
1.3.1 Medicines derived from plants
With a long history of plants in traditional medicine, many compounds from plants have entered clinical use. In addition to quinine, paclitaxel and artemisinin, the anticancer drug camptothecin (1.9) was isolated from the bark and stems of the Chinese tree
Camptotheca acuminate.10 Galantamine (1.10) from the plant, Galanthus nivalis, is used to treat Alzheimer’s disease,11 while dronabinol (D9-THC) (1.11) and cannabidiol (1.12), which are used as adjunctive treatments for the symptomatic relief of neuropathic pain, were obtained from the Cannabis plant.12
Figure 1.4 Chemical structures of 1.9-1.12
1.3.2 Medicines derived from microorganisms
4 Microorganisms became an important source for natural product drug dicovery beginning with the success of the penicillins in treating infections. Fumagillin (1.13) was isolated from Aspergillus fumigatus and approved for use in the treatment of intestinal microsporidiosis in France in 2005.10 Depsipeptide (FR-901228, FK-228) (1.14) is an inhibitor of HDAC (histone deacetylase). It is isolated from Chromobacterium violaceum and now in phase II clinical trials for treating cancers.13 Marketed in Japan since 2002, amrubicin (1.15), a derivative of doxorubicin isolated from Streptomyces peucetius var caesius, is used in the treatment of lung cancer by inhibiting topoisomerase II.14
Figure 1.5 Chemical structures of 1.13-1.15
1.3.3 Medicines derived from marine organisms
Water covers more than 70% of the earth, but it was only in the 1950s that scientists started to search for medicinal natural products from the sea. The approval of spongouridine (1.16) and spongothymidine (1.17) from the Caribbean sponge
Cryptotheca crypta as anticancer drugs, catalyzed increased work on the discovery of marine natural products.15,16 Ziconotide (1.18), derived from the toxin of the cone snail species Conus magus, is used as a treatment for patients suffering from chronic pain.
5 Ziconotide acts by blocking the N-type calcium channel to inhibit the release of pro- nociceptive neurochemicals. 17
Figure 1.6 Chemical structures of 1.16-1.18
Trabectedin (1.19) is a tetrahydroisoquinoline alkaloid isolated from the ascidian
Ecteinascidia turbinata. It was approved by EMEA (European Medicines Agency) in
2007 for the treatment of advanced soft tissue sarcoma. Trabectedin is also in clinical trials for treating ovarian, breast and prostate cancers.18,19 Isolated from the bryozoan
Bugula neritina, bryostatin 1 (1.20) is a protein kinase C inhibitor. It currently in phase II clinical trials for cancer treatment.16,20
Figure 1.7 Chemical structures of 1.19 and 1.20
1.3.4 Medicines derived from terrestrial animals
6 Some compounds obtained from various terrestrial vertebrates and invertebrates could also be potential medicines due to their biochemical properties. Exenatide (1.21), a
39 amino acid peptide isolated from the saliva of the Gila monster Heloderma suspectum, acts as an adjunctive therapy to treat type 2 diabetes by improving glycemic control.21,22
The skin of the frog Epipedobates tricolor in Ecuador yielded epibatidine (1.22), which has been isolated and used as a lead compound for developing drugs for pain relief.23,24
Figure 1.8 Chemical structures of 1.21 and 1.22
1.4 Anticancer agents and antimalarial agents from natural products
1.4.1 Anticancer agents
Cancer is a class of diseases that causes millions of human deaths each year. It particularly refers to the rapid growth of abnormal cells, cell invasion to adjacent tissues, and sometimes spreading of abnormal cells to other locations. The World Trade
Organization (WHO) estimates 7.9 million deaths were due to cancer in 2007 around 13% of all deaths.25
The Search for anticancer drugs from natural products began in the late 1940s. Other than paclitaxel (1.7), there are several anticancer drugs available derived from natural products. Topotecan (1.23) and irinotecan (1.24) are semisynthetic, water soluble analogs of camptothecin (1.9). Topotecan was approved by the FDA in 1996 and is used as second-line therapy for advanced ovarian cancer. Irinotecan was approved in 2000 by
7 FDA for treating advanced colorectal cancer. By stabilizing the DNA-topoisomerase I covalent binary complex, topotecan and irinotecan inhibit DNA synthesis and cause cell death during the cell cycle.20
Figure 1.9 Chemical structures of 1.23 and 1.24
Isolated from Streptomyces peucetius derived strains, doxorubicin (1.25) was registered in the early 1970s and has been one of the most widely used drugs in cancer chemotheraphy. Its analog epirubicin (1.26), with better performance to doxorubicin in clinical trial, was approved by the FDA in 1999. Doxorubicin and epirubicin are used to treat different cancers by interacting with DNA and inhibiting topoisomerase II.20, 26
Figure 1.10 Chemical structures of 1.25 and 1.26
8 Vinca alkaloids represent one of the most important classes of anticancer agents. In distinction to paclitaxel, which acts as a promoter of tubulin polymerization, vinca alkaloids inhibit the assembly of tubulin into microtubules, and prevent the cells from undergoing division. Vinblastine (1.27) and vincristine (1.28) were isolated from the leaves of Cantharanthus roseus, a plant endemic to Madagascar, in the 1950s. Since the
1960s, the vinca alkaloids vinblastine and vincristine have been widely used for treating different types of cancer.20, 26
Figure 1.11 Chemical structures of 1.27 and 1.28
1.4.2 Antimalarial agents
Malaria is a mosquito-borne infectious disease caused by an eukaryotic protist of the genus Plasmodium. The disease is found in tropical regions throughout sub-Saharan
Africa, Southeast Asia, the Pacific Islands, India, and Central and South America. About half of the world’s population lives in malaria-endangered areas. In 2008, malaria kills nearly one million people out of 250 million malaria cases. A child dies of malaria every
30 seconds.27 Medicines for treating malaria are needed urgently.
9 Powdered bark from the cinchona tree containing the plasmodicidal quinoline alkaloids quinine (1.1) and quinidine (1.29) was the first antimalarial medicine and used to treat malarial for centuries.6
Figure 1.12 Chemical structures of 1.29-1.31
Chloroquine (1.30) was synthesized in 1934 and became the foundation of malaria therapy after World War II. However, a drug resistant strain began to emerge in the
1960s. The new antimalarial agent called artemisinin (1.8) was isolated in 1972 from
Chinese herb Artemisia annua, which is a plant used in traditional Chinese medicine for malaria treatment.6 Artemisinin is currently one of the most efficient antimalarial medicines in the market. Arterolane (OZ-277) (1.31), a synthetic trioxolane modelled on the artemisinin pharmacophore, is in phase II clinical trial.8
However, the growing threat of drug resistant strains due to gene mutation demands a more extensive searching for new antimalarial agents from natural products.
1.5 The ICBG (International Cooperative Biodiversity Groups) program
The tropical rain forests of the world provide a huge source for natural products, holding the potential for drug discovery. However, in the past several decades, those
10 forests are disappearing at a rapid rate, especially in developing countries, because of continuous logging and conversion of forests to agricultural uses.
In an attempt to address the interdependent issues of drug discovery, biodiversity conservation and economic development, the International Cooperative Biodiversity
Groups (ICBG) Program was initiated in 1993. It is currently jointly funded by the U. S.
National Institutes of Health (NIH), the National Science Foundation (NSF), the U. S.
Department of Agriculture (USDA), the U. S. Department of Energy (DOE) and the
National Oceanic and Atmospheric Administration (NOAA).28
The Kingston research group at Virginia Tech has been funded by the ICBG program since 1993, and has been collaborating with the Missouri Botanical Garden, Conservation
International, the three Madagascar centers Center National d’Application des
Recherches Pharmaceutiques (CNARP), Centre National de Recherche Oceanographique,
(CNRO), and Centre National de Recherche pour l’Environnement (CNRE) to study tropical plants, marine organisms and microorganisms in Madagascar. The companies
Eisai Inc. and Dow AgroSciences are also partners in this cooperative program.
As the fourth biggest island in the world, Madagascar has been isolated from Africa for over 150 million years. For this reason, 75% of the 200,000 plant and animal species found in Madagascar are endemic to the island. The Madagascar ICBG program is searching for new anticancer agents and antimalarial agents by isolation of bioactive natural products from different resources in Madagascar.
Plants, marine organisms and microorganisms are collected and screened for antimalarial activity in Madagascar, and screened for antiproliferative activity at Virginia
Tech. Potential active extracts are selective for fractionation and purification under the
11 guidance of bioassay. In the past twelve years, many new active antiproliferative compounds have been isolated. Due to the great biodiversity of the flora and fauna of
Madagascar, the isolated compounds covered various types of natural products from alkaloids, flavonoids to terpenoids and many others with all kinds of skeletons.29 The structural variety of the isolated compounds may increase the hit rate in the bioassay screening and be beneficial for drug discovery.
Searching for novel bioactive agents from Madagascar is the project which this dissertation will describe. The research was focused on the isolation and structure elucidation of bioactive natural products applying bioassay guided fractionation and modern analytical techniques including LC-MS, NMR, UV, IR, optical rotation and CD
(circular dichroism). The A2780 human ovarian cancer cell line was the most frequently used bioassay in addition to the occasional use of other cancer cell lines. HB3 and Dd2 malaria parasites were the most used bioassays for antimalarial extracts. This dissertation also involves the synthesis of bioactive natural products, specifically two new antiproliferative alkaloids from a Malagasy plant. The research work is discussed in detail in the following chapters.
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14 venom - further evidence for an exendin receptor on dispersed acini from guinea-pig
pancreas. J. Biol. Chem. 1992, 267, 7402-7405.
23. Spande, T. F.; Garraffo, H. M.; Edwards, M. W.; Yeh, H. J. C.; Pannell, L.; Daly, J.
W., Epibatidine - a novel (chloropyridyl)azabicycloheptane with potent analgesic
activity from an Ecuadorian poison frog. J. Am. Chem. Soc. 1992, 114, 3475-3478.
24. Carroll, F. I., Epibatidine structure-activity relationships. Bioorg. Med. Chem. Lett.
2004, 14, 1889-1896.
25. http://www.who.int/cancer/en/ accessed 12/6/10.
26. da Rocha, A. B.; Lopes, R. M.; Schwartsmann, G., Natural products in anticancer
therapy. Curr. Opin. Pharmacol. 2001, 1, 364-369.
27. http://www.who.int/features/factfiles/malaria/en/index.html accessed 12/6/10.
28. http://www.icbg.org/ accessed 12/6/10.
29. Hou, Y.; Harinantenaina, L., New and bioactive natural products isolated from
Madagascar plants and marine organisms. Curr. Med. Chem. 2010, 17, 1191-1219.
15 II. An antiproliferative xanthone and two guttiferones of Symphonia tanalensis from
the Madagascar rainforest1
This chapter is a slightly expanded version of a published article.2 Attributions from co-authors of the article are described as follows in the order of the names listed. The author of this dissertation (Mr. Ende Pan) conducted isolation and structure elucidation of the titled compounds, and drafted the manuscript. Dr. Shugeng Cao was a mentor for this work, and in particular, he provided invaluable advice and hints for structure elucidation of those compounds, and he also proofread the manuscript before submission. Ms. Peggy
Brodie performed the A2780 bioassay on the isolated fractions and compounds. Dr.
James S. Miller, Dr. Fidisoa Ratovoson and Dr. Chris Birkinshaw from Missouri
Botanical Garden did the plant collections and identification. Dr. Roland
Rakotondrajaona, Dr. Rabodo Andriantsiferana and Dr. Vincent E. Rasamison from
Madagascar carried out the initial plant extraction. Dr. David G. I. Kingston was a mentor for this work and the corresponding author for the published article. He provided critical suggestions for this work and crucial revisions to the manuscript.
2.1 Introduction
In our continuing search for biologically active natural products from tropical rainforests as part of an International Cooperative Biodiversity Group (ICBG) program,1,3 we obtained an ethanol extract of a plant identified as Symphonia tanalensis Jum. & H.
Perrier (Clusiaceae) from Madagascar. The extract showed moderate antiproliferative activity against the A2780 human ovarian cancer cell line with an IC50 value of 19 g/mL.
16 On the basis of the activity and the absence of previous phytochemical study on this species, S. tanalensis was selected for further investigation under the guidance of our bioassay.
2.1.1 Previous investigations of Symphonia
Previous studies on the genus Symphonia reported guttiferone analogues4-6 and xanthone derivatives,5-11 some of which showed anti-HIV,4 antioxidant5 and anti- plasmodial5, 7, 10-11 activities. The cytotoxicities of xanthones9, 12 and guttiferones13-14 have also been studied. Bioassay-guided fractionation by applying C18 open column and HPLC on the dichloromethane fraction yielded a new xanthone 2.1 and two guttiferones 2.2 and
2.3. Herein we report the structural elucidation of the new xanthone and the antiproliferative properties (against the A2780 human ovarian cancer cell line) of the isolated compounds.
Figure 2.1 Compounds isolated from Symphonia.
17 2.2 Results and Discussion
2.2.1 Structure elucidation of compound 2.1
Compound 2.1 was obtained as a yellow solid. Its positive ESI-MS revealed a pseudomolecular ion peak at m/z = 425.1590 [M+H]+ corresponding to a molecular
1 formula of C24H25O7, (calcd for C24H25O7 425.1600). The H NMR spectrum showed signals for one aromatic proton singlet at δH 7.21 (s), one methoxyl group at δH 3.95 (s), an olefinic proton at δH 5.22 (t, J = 7.5 Hz), two methylene protons at δH 3.35 (d, J = 7.5
Hz) and a pair of downfield methyl singlets at δH 1.68 (s) and 1.82 (s) which corresponded to a 3-methyl-2-butenyl group. In addition a pair of cis-coupled olefinic doublets (δH 7.04, d, J = 10.0 Hz; 5.69, d, J = 10.0 Hz) and a pair of methyl singlets at δH
1.48 (s) suggested the presence of a 3-oxygenated-3-methyl-butenyl group.
The 13C NMR spectrum of 2.1 exhibited 24 carbon signals (Table 2.1), including one resonances of a carbonyl at δC 181.7, one methoxyl group at δC 56.6, one 3-methyl-2- butenyl group (δC 22.0, 123.5, 131.9, 26.0, 18.1), and one 3-oxygenated-3-methyl- butenyl group (δC 116.6, 127.8, 79.1, 28.5, 28.5), as well as twelve aromatic carbons assignable to two isolated aromatic rings, seven of which were oxygenated due to their corresponding down field carbon chemical shifts (δC 160.8, 151.4, 159.0, 134.4, 143.8,
143.3, 147.3). The above data suggested that compound 2.1 was a xanthone similar to those previously isolated from the same genus Symphonia.5-11 In order to satisfy the degree of unsaturation number (13) implied by the molecular formula C24H24O7, the 3- oxygenated-3-methyl-butenyl group must be cyclized with a hydroxyl group of the xanthone moiety to form a gem-dimethyl dihydropyran. To assign the attachment of the functionalities, 2D NMR experiments were carried out.
18 1 13 a Table 2.1 H and C NMR Data (δ) in CD3OD for Compounds 2.1
position δH δC
1 160.8
2 113.1
3 151.4
4 103.5
4a 159.0
5 134.4
5a 143.8
6 143.3
7 147.3
8 7.21 (s) 96.7
8a 112.3
9 181.7
9a 102.2
1' 3.35 (d, 7.5) 22.0
2' 5.22 (t, 7.5) 123.5
3' 131.9
4' 1.68 (s) 26.0
5' 1.82 (s) 18.1
1'' 7.04 (d, 10.0) 116.6
2'' 5.69 (d, 10.0) 127.8
3'' 79.1
4'' 1.48 (s) 28.5
5'' 1.48 (s) 28.5
OCH3 3.95 (s) 56.6 a δ (ppm) 500 MHz for 1H and 125 MHz for 13C; multiplicities, J values (Hz) in parentheses.
HMQC (Heteronuclear Multiple Bond Coherence) allowed the assignment of all the
19 signals observed in the 1D NMR spectra. Careful interpretation of the 1H–1H COSY,
HMBC, ROESY and NOESY allowed us to confirm the xanthone nature of 2.1.
Figure 2.2 Chemical structure of compound 2.1.
1 1 H- H COSY correlations between the signal of H-1" (H7.04) and H-2" (H 5.69) as well as the HMBC correlations between H-1" and the two oxygenated aromatic carbons:
C-3 δC 151.4, C-4a δC 159.0 led us to conclude that the 3,3-dimethylpyrano ring was attached at C-3 and C-4. The allocation of the proton on the pentasubstituted aromatic ring was substantiated by the observation of 3J correlations between the singlet aromatic proton at H-8 (H 7.21) and the carbonyl carbon C-9, the two overlapped aromatic
2 carbon signals at C-5a (C 143.8) and at C-6 (C 143.3), and J correlations between H-8
(H 7.21) and the aromatic carbon at C-8a (C 112.3) and C-7 (C 147.3). The location of a methoxyl group on C-7 was deduced from the cross peak in the ROESY spectrum between the signals at δH 3.95 and at H-8. This was confirmed by the observation of
HMBC correlation between the signal of the methoxyl group and that of C-7.
20 OH COSY O 4 O OH HMBC ROESY 1 O OH O H NOESY . . Figure 2.3 NMR correlations of compound 2.1.
The hydrogen bonded proton observed at δH 13.30 in CDCl3 indicated a hydroxyl
1 1 group must be attached on C-1. H- H COSY correlations between the signal at H 3.35
(H2-1') and that at H 5.22 (H-2') as well as a HMBC long-range coupling between the signal at H-2’ and those of two methyl groups (H 1.68 and H 1.82) confirm the presence of a 3-methyl-2-butenyl group. The latter was assigned to be attached at the C-2 position due to the HMBC correlations observed from H-1' to C-1 and to C-2. Furthermore, a clear NOESY coorelation was observed between the hydrogen-bonded proton and H2-1' in the spectrum of 2.1 measured in CDCl3. On the basis of the molecular formula of 2.1, the remaining two hydroxyl groups must be located at C-5 and C-6. From the above data, the structure of 2.1 was determined to be 2-(3,3-dimethylallyl)-1,5,6-trihydroxy-7- methoxy-2'',2''-dimethylpyrano(6'',5'':3,4) xanthone.
2.2.2 Identification of the known guttiferone I and A
The structures of the two known guttiferone analogues (guttiferone I (2.2) and guttiferone A (2.3) ) isolated in the present study were deduced by comparison with data reported in the literature.4, 15
21
Figure 2.4 Chemical structures of compounds 2.2 and 2.3.
2.2.3 Antiproliferative activities of isolated compounds
Compound 2.1 showed a strong antiproliferative activitiy against the A2780 human ovarian cancer cell lines with an IC50 value of 3.8 μM. Lenta and co-workers isolated a very similar compound [2-(3,3-dimethylallyl)-1,5-dihydroxy-6,7-dimethoxy-2'',2''- dimethylpyrano (6'',5'':3,4)xanthone] (2.4) which showed good anti-plasmodial activity
(1.3 μM).5, 7 Although the cytotoxic activities of xanthones (against KB, MCF-7, HT-29,
Hela, et al) 16-18 have been reported in the literature, only few reports have investigated the properties of xanthones against the A2780 human ovarian cancer cell lines.19
Compound 2.1 showed better antiproliferative activity than two xanthones (2.5 and 2.6) previously reported in the literature.19
Figure 2.5 Chemical structures of compound 2.4-2.6.
22 Compounds 2.2 and 2.3 showed IC50s of 8.3 μM and 7.8 μM antiproliferative activity against the A2780 cell line.
2.3 Experimental Section
2.3.1. General experimental procedure
UV spectra were measured on a Shimadzu UV-1201 spectrophotometer. NMR spectra were recorded in CD3OD on either Varian INOVA 400 or JEOL Eclipse 500 spectrometers. The chemical shifts are given in δ (ppm) and coupling constants (J) are reported in Hz. Mass spectra were obtained on an Agilent 6220 TOF Mass Spectrometer.
HPLC was performed on a Shimadzu LC-10AT instrument with a semi-preparative C18
Varian Dynamax column (5m, 250 mm, 10 mm) and a preparative C18 Varian Dynamax column (8 μm, 250 mm, 21.4 mm). Optical rotation was measured on a JASCO P-2000 polarimeter.
2.3.2. Antiproliferative bioassay
Antiproliferative activities were obtained at Virginia Polytechnic Institute and State
University against the drug-sensitive A2780 human ovarian cancer cell line. Human ovarian cancer cells (A2780) grown to 95% confluency were harvested and re-suspended in growth medium (RPMI1640 supplemented with 10% fetal bovine serum and 2 μM L- glutamine). Cells were counted using a hemacytometer and a solution containing 2.5x105 cells per ml was prepared in growth media. Eleven columns of a 96 well microtitre plate were seeded with 199 μL of cell suspension per well, and the remaining column contained media only (one hundred percent inhibition control).
23
Scheme 2.1 96-well plate of A2780 cell line
The plate was incubated for 3 hours at 37ºC/5% CO2 to allow the cells to adhere to the wells. Following this incubation, potential cytotoxic agents, prepared in DMSO, were added to the wells in an appropriate series of concentrations, 1 μL per well. One column of wells was left with no inhibitor (zero percent inhibition control), and 4 dilutions of taxol (IC50 0.015 g/mL) were included as a positive control. (Scheme 2.1) The plate was incubated for 2 days at 37ºC/5% CO2, then the media gently shaken from the wells and replaced with reaction media (supplemented growth medium containing 1% Alamar blue), and incubated for another 3 hours. The level of Alamar blue converted to a fluorescent compound by living cells was then analyzed using a Cytofluor Series 4000 plate reader (Perseptive Biosystems) with an excitation wavelength of 530 nm, an emission wavelength of 590 nm, and gain of 45. The percent inhibition of cell growth
24 was calculated using the zero percent and one hundred percent controls present on the plate, and an IC50 value (concentration of cytotoxic agent which produces 50% inhibition) was calculated using a linear extrapolation of the data which lie either side of the 50% inhibition level. Samples were analyzed in triplicate on at least two separate occasions to produce a reliable IC50 value. The A2780 cell line is a drug-sensitive ovarian cancer cell line.20
2.3.3. Plant material
A sample of the leaves, fruit and inflorescence of Symphonia pauciflora Baker
(Clusiaceae) was collected in October 2000 as ROL 136. The collection was made by
Rolland Rakotodrajaona et al. from a plant growing in dense humid forest near the Parc
National de Zahamena at coordinates 17°33′15″S 48°53′23″ E at an elevation of 730 m.
Voucher specimens have been deposited at the Parc Botanique and Zoologique de
Tsimbazaza (TAN) and at the Centre National d'Application des Recherches
Pharmaceutiques (CNARP) in Antananarivo, Madagascar; the Missouri Botanical Garden in St. Louis, Missouri (MO); and the Muséum National d'Histoire Naturelle in Paris,
France (P).
2.3.4. Extraction and Isolation
Dried leaves, inflorescence and fruits of Symphonia tanalensis (370 g) were ground in a hammer mill, then extracted with ethanol by percolation for 24 hours at room temperature to give the crude extract MG 0724 (35.5 g), of which 7.8 g was shipped to
Virginia Polytechnic Institute and State University (VPISU) for further bioassay guided
25 isolation. The fractionation tree is shown in Scheme 2.2. The extract MG 0724 (IC50: 19
μg/mL, 2.3 g) was suspended in aqueous MeOH (MeOH-H2O, 9:1, 100 mL) and extracted with hexane (3 x 100 mL portions). The aqueous layer was then diluted to 60%
MeOH (v/v) with H2O and extracted with CH2Cl2 (3 x 150 mL portions). The hexane extract was evaporated in vacuo to leave 242 mg with an IC50 value of 11 μg/mL. 1.04 g of residue from CH2Cl2 extract also showed IC50: 11 μg/mL activity. The aqueous MeOH extract (998 mg) was inactive. The CH2Cl2 extract was first fractionated by a C18 open
Scheme 2.2 Separation of ethanol extract of Symphonia tanalensis
26 column, and four fractions were collected. Fractions A, B, C, and D (61, 748, 134, and
156 mg) had IC50 values of 20, 10, 16, and >25 μg/mL, respectively. Fraction B was separated by C18 preparative HPLC (90% MeOH-H2O), and 11 sub-fractions were obtained. Using Diol semi-preparative HPLC (5% EtOH in DCM), compound 2.1 (1.4 mg, tR 19.8 min) was purified from sub-fraction B-5. Compounds 2.2 (0.9 mg, tR 25.1 min) and 2.3 (1.2 mg, tR 27.2 min) were isolated from sub-fraction B-9 by Diol semi- preparative HPLC (5% EtOH in DCM).
2.3.5 2-(3,3-Dimethylallyl)-1,5,6-trihydroxy-7-methoxy-2'',2''-dimethylpyrano (6'',5'':3,4) xanthone (2.1)
Yellow solid; UV (MeOH) λmax nm (log ε) 206 (4.37), 219 (4.33), 271 (4.31), 337
1 13 (3.70), 376 (3.82) nm; H NMR (500 MHz, CD3OD) and C NMR (125 MHz, CD3OD),
+ see table 2.1; ESI-MS m/z 425.1590 [M+H] (calcd for C24H25O7 :425.1600)
2.3.6 Guttiferone I (2.2)
Yellow solid; [α]D -64 (c 0.2 CHCl3) UV (MeOH) λmax nm (log ε) 235 (4.05), 279
1 (4.15) nm; H NMR (500 MHz, CD3OD) 7.20 (d, J = 1.9 Hz, H-12), 6.96 (dd, J = 8.3, 1.9
Hz, H-16), 6.67 (d, J = 8.3 Hz, H-15), 5.16 (m, H-30), 5.06 (m, H-25), 4.90 (m, H-18),
2.72 (dd, J = 9.1, 13.5 Hz, H-17a), 2.55 (m, H-17b), 2.51 (m, H-29), 2.11 (m, H-24), 2.04
(m, H-7), 2.00 (m, H-32), 2.00 (m, H-33), 1.98 (m, H-6), 1.69 (s, H-38), 1.68 (s, H-21),
1.64 (s, H-20), 1.64 (s, H-27), 1.57 (s, H-36), 1.53 (s, H-37), 1.48 (s, H-28), 1.23 (s, H-
13 22), 1.01 (s, H-23) and C NMR (125 MHz, CD3OD) (209.8 (C-9), 196.0 (C-10), 195.3
(C-3), 195.0 (C-1), 152.6 (C-14), 146.2 (C-13), 139.2 (C-31), 135.7 (C-19), 133.8 (C-26),
27 132.3 (C-35), 129.5 (C-11), 125.5 (C-25), 125.3 (C-16), 125.3 (C-34), 120.8 (C-18),
120.5 (C-30), 117.9 (C-2), 117.3 (C-12), 115.1 (C-15), 68.0 (C-4), 61.7 (C-8), 47.8 (C-5),
41.0 (C-6), 41.0 (C-32), 40.7 (C-7), 31.8 (C-29), 30.1 (C-24), 27.7 (C-33), 27.3 (C-23),
27.1 (C-17), 26.4 (C-27), 26.0 (C-20), 26.0 (C-36), 23.3 (C-22), 18.3 (C-21), 18.2 (C-28),
+ 17.8 (C-37), 16.8 (C-38); ESI-MS m/z 603.3694 [M+H] (calcd for C38H51O6, 603.3686).
2.3.7 Guttiferone A (2.3)
Yellow solid; [α]D +28 (c 0.1 CHCl3) UV (MeOH) λmax nm (log ε) 235 (4.05), 280
1 (4.14) nm; H NMR (500 MHz, CD3OD) 7.21 (d, J = 2.0 Hz, H-12), 7.01 (dd, J = 8.4, 2.0
Hz, H-16), 6.69 (d, J = 8.3 Hz, H-15), 5.21 (m, H-30), 5.08 (m, H-35), 2.65 (m, H-17a),
2.61 (m, H-17b), 2.47 (m, H-29a), 2.44 (m, H-29b), 2.09 (m, H-7b), 2.09 (m, H-24a),
2.06 (m, H-24b), 1.97 (m, H-7a), 1.87 (m, H-34), 1.83 (m, H-6), 1.71 (s, H-32), 1.68 (s,
H-21), 1.67 (s, H-33), 1.67 (s, H-37), 1.66 (s, H-27), 1.64 (s, H-27), 1.60 (s, H-20), 1.52
(s, H-28), 1.39 (m, H-23a), 1.24 (s, H-22), 1.20 (m, H-23b) and 13C NMR (125 MHz,
CD3OD) 209.7 (C-9), 195.6 (C-10), 195.6 (C-1), 195.5 (C-3), 152.6 (C-14), 146.2 (C-13),
135.7 (C-19), 135.7 (C-31), 133.8 (C-26), 132.9 (C-36), 129.3 (C-11), 125.5 (C-25),
125.1 (C-35), 125.0 (C-16), 120.8 (C-18), 120.8 (C-30), 117.8 (C-2), 117.4 (C-12), 115.2
(C-15), 68.8 (C-4), 62.1 (C-8), 51.9 (C-5), 41.1 (C-6), 40.1 (C-7), 36.9 (C-23), 32.0 (C-
29), 29.8 (C-24), 26.6 (C-17), 26.3 (C-27), 26.3 (C-32), 26.0 (C-20), 25.9 (C-37), 23.6
(C-34), 19.6 (C-22), 18.3 (C-33), 18.2 (C-21), 18.1 (C-28), 17.7 (C-38); ESI-MS m/z
+ 603.3691 [M+H] (calcd for C38H51O6, 603.3686).
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32 III. Four diphenylpropanes and a cycloheptadibenzofuran from
Bussea sakalava from the Madagascar Dry Forest1
This chapter is a slightly expanded version of a published article.2 Attributions from co-authors of the article are described as follows in the order of the names listed. The author of this dissertation (Mr. Ende Pan) conducted isolation and structure elucidation of the titled compounds, and drafted the manuscript. Dr. Liva Harinantanaina was a mentor for this work, and in particular, he provided invaluable advice and hints for structure elucidation of those compounds, and he also proofread the manuscript before submission.
Ms. Peggy Brodie performed the A2780 bioassay on the isolated fractions and compounds. Dr. James S. Miller, Dr. Richard Randrianaivo, Dr. Fidisoa Ratovoson and
Dr. Chris Birkinshaw from Missouri Botanical Garden did the plant collections and identification. Dr. Rabodo Andriantsiferana and Dr. Vincent E. Rasamison from
Madagascar carried out the initial plant extraction. Dr. David G. I. Kingston was a mentor for this work and the corresponding author for the published article. He provided critical suggestions for this work and crucial revisions to the manuscript.
3.1 Introduction
In our continuing search for biologically active natural products from tropical rainforests as part of an International Cooperative Biodiversity Group (ICBG) program, we obtained an ethanol extract from the roots of a plant identified as Bussea sakalava Du
Puy & R. Rabev. (Fabaceae) from Madagascar. This extract showed moderate antiproliferative activity against the A2780 human ovarian cancer cell line with an IC50
33 value of 10 g/mL. The extract was selected for examination on the basis of this activity and the absence of previous phytochemical studies of the species.
3.1.1 Previous investigations of Bussea
Previous studies on the genus Bussea indicated the presence of azetidine-2-carboxylic acid and 3-hydroxyproline in seeds of different Bussea species,3-4 and the cytotoxicity and high trypanocidal activity of a methanol extract of stem bark of Bussea occidentalis has been reported.5
Figure 3.1 Compounds isolated from Bussea
3.2 Results and Discussion
Fractionation of a dichloromethane fraction of an ethanol extract of B. sakalava by
C18 open column and high performance liquid chromatography (HPLC) yielded four new diphenylpropanes named bussealins A-D (3.1-3.4) and a cycloheptadibenzofuran derivative named bussealin E (3.5). Herein we report the structural elucidation of these new compounds and their antiproliferative properties against the A2780 human ovarian cancer cell line.
3.2.1 Structure elucidation of bussealin A (3.1)
34 Bussealin A (3.1) was obtained as an off-white solid. Its positive ESI-MS revealed a pseudomolecular ion peak at m/z 321.1338 [M + H]+ corresponding to molecular formula
-1 C17H21O6. The IR spectrum showed absorptions of OH (3367 cm ) and aromatic groups.
1 The H NMR spectrum (Table 3.1) exhibited a singlet at δH 6.18 (s, 2H) corresponding to a pair of aromatic protons of an A2 system, two aromatic doublets [δH 6.50 (d, J = 8.4 Hz) and 6.38 (d, J = 8.4 Hz)] of an AB system, two OCH3 groups [δH 3.75 (s) and 3.78 (s)], and a multiplet and two triplet methylene groups at δH 1.79 (m, 2H), 2.52 (t, J = 7.7 Hz,
2H) and 2.41 (t, J = 7.7 Hz, 2H) respectively. The 13C NMR spectrum of 3.1 exhibited signals for 17 carbons, including three methylene carbons (δC 36.5, 33.0, and 30.6), two
OCH3 groups (δC 56.5 and 60.8), and twelve aromatic carbons assignable to two isolated aromatic rings. Six of the aromatic carbons were oxygenated, as shown by their deshielded carbon chemical shifts (Table 3.1) and were consistent with the molecular formula. The above data suggested that 3.1 had a diphenyl propane skeleton. The complete 1H and 13C NMR assignments and the connectivities were determined from analysis of a combination of COSY, HMQC, and HMBC data. Three mutually coupled methylene groups were revealed by the cross peaks observed in the COSY spectrum. In the HMBC spectrum, H-1 (δH 2.41) showed correlations with C-2 (δC 33.0), C-3 (δC
30.6), C-1' (δC 140.2), and with C-2' and C-6', both of which had the same chemical shifts
(δC 108.7). The A2 substitution pattern of the A ring of 3.1 was established by HMBC correlations from the signal at δH 6.18 (H-2' and H-6') to C-1 (δC 36.5), C-1' (δC 140.2),
C-3' (δC 151.3), C-4' (δC 134.7) and C-6' and C-2' (δC 108.7), as well as the correlation from one OCH3 group at δH 3.75 to C-4' (δC 134.7). The proton substitutions on the B
3 ring were assigned based on the J HMBC correlations between H-3 (δH 2.52) and C-6''
35 (δC 120.5), and between H-5'' (δH 6.38) and C-1'' (δC 123.4). Moreover, the H-5'' proton showed HMBC correlations to C-6'' (δC 120.5), C-4'' (δC 147.8) and C-3'' (δC 134.9). The location of the remaining OCH3 group was at C-4'', as deduced from the HMBC correlation between the signal at δH 3.78 and that of C-4''. On the basis of the molecular formula of 3.1, the remaining four OH groups were located at C-2'' (δC 144.7), C-3'' (δC
134.9), C-3' (δC 151.3), and C-5' (δC 151.3). Bussealin A is thus assigned the structure
3',5',2'',3''-tetrahydroxy-4',4''-dimethoxy-1,3-diphenylpropane (3.1).
Figure 3.2 Chemical structure of bussealin A-D (3.1-3.4)
3.2.2 Structure elucidation of bussealin B (3.2)
Bussealin B (3.2) was obtained as an off-white solid. Its positive ESI-MS revealed a pseudomolecular ion peak at m/z 335.1512 [M + H]+ corresponding to molecular formula
1 C18H23O6. The H NMR spectrum (Table 1) showed two singlets of an AX system at δH
6.58 (s) and 6.60 (s), two aromatic doublets of an AB system at δH 6.51 (d, J = 8.4 Hz)
36 and 6.39 (d, J = 8.4 Hz), three OCH3 groups [δH 3.76 (s), 3.80 (s) and 3.83 (s)], and one multiplet and two triplet methylene groups at δH 1.76 and 2.54 (t, J = 7.8 Hz) and 2.50 (t,
J = 7.8 Hz). Inspection of the 1H and 13C NMR spectra of 3.2 revealed close similarities with those of 3.1, except for the presence of an additional OCH3 signal and the chemical shifts of the AX system of ring A. The fact that the chemical shifts of the carbons of ring
B of compounds 3.1 and 3.2 were superimposable (Table 3.1) indicated the presence of a
2'',3''-dihydroxy-4''-methoxyphenyl group in 3.2. Interpretation of HMBC and NOESY experiments allowed us to determine the location of the OCH3 groups to be at 2', 4', and
4''. The two singlet aromatic protons on ring A were assigned according to the
3 observation of J HMBC correlations from H-6' (δH 6.60) to C-1 (δC 30.4) and from H-3'
(δH 6.58) to C-1' (δC 124.7). Moreover, the proton signal of H-1 (δH 2.50) showed HMBC correlations with C-1' (δC 124.7), C-6' (δC 117.9) and the methoxylated carbon at C-2' (δC
152.2). This indicated that the third OCH3 group must be at C-4' or C-5'. NOESY correlations from H-3' (δH 6.58) to 2'-OMe (δH 3.76) and to 4'-OMe (δH 3.83) established the location of the methoxy group at C-4' and the hydroxy group at C-5'. The structure of bussealin B was thus assigned as 5',2'',3''-trihydroxy-2',4',4''-trimethoxy-1,3- diphenylpropane.
3.2.3 Structure elucidation of bussealin C (3.3)
Bussealin C (3.3) was obtained as an off-white solid. Its positive ESI-MS revealed a pseudomolecular ion peak at m/z 305.1384 [M + H]+ corresponding to molecular formula
1 13 C17H21O5. Its H NMR and C NMR spectra (Table 3.1) indicated that 3.3 is also a diphenylpropane with a 2'',3''-dihydroxy-4''-methoxyphenyl group substituted at C-3. The
37 1,3,4-trisubstituted A ring was determined by the proton coupling constants and HMBC correlations from H-2' (δH 6.64) and H-6' (δH 6.60) to C-1 (δC 36.1), and COSY correlations between H-5' (δH 6.79) and H-6' (δH 6.60). Furthermore, the HMBC
3 spectrum showed a J correlation from H-6' to the methoxylated carbon at C-4' (δC 147.0), which was confirmed by NOESY correlations between H-5' (δH 6.79) and 4'-OMe (δH
3.80). The above data coupled with the molecular formula led to assignment of the structure of bussealin C as 3',2'',3''-trihydroxy-4',4''-dimethoxy-1,3-diphenylpropane.
3.2.4 Structure elucidation of bussealin D (3.4)
Bussealin D (3.4) was obtained as an off-white solid. The positive ESI-MS exhibited a pseudomolecular ion peak at m/z 349.1648 [M + H]+ corresponding to the molecular
1 13 formula C19H25O6. The H NMR and C NMR spectra (Table 3.1) indicated that 3.4 had the same tetrasubstituted B ring with an OCH3 group at C-4'' as in compounds 3.1-3.3. In its 1H NMR spectrum, the coupling patterns and the locations of the aromatic proton resonances of ring A were very similar to those of 3.2.
The presence of three OCH3 groups and the substitution pattern of ring A of compound 3.4 were deduced by interpretation of the 1D and 2D NMR data. The HMBC spectrum of 3.4 showed correlations from H-1 (δH 6.79) to C-1' (δC 124.3), C-6' (δC
3 116.3) and to the methoxylated carbon at C-2' (δC 153.3). Furthermore, a clear J long- range correlation from the singlet proton H-3' (δH 6.61) to C-1' (δC 124.3) was also observed. Thus, the two remaining OCH3 groups were determined to be at C-4' (δC 149.1) and C-5' (δC 144.1). The structure of bussealin D was thus determined to be 2'',3''- dihydroxy-2',4',5',4''-tetramethoxy-1,3-diphenylpropane.
38
1 13 a Table 3.1 H and C NMR data for Bussealin A-D (3.1-3.4) in CD3OD
3.1 3.2 3.3 3.4 position 1H (J, Hz) 13C 1H (J, Hz) 13C 1H (J, Hz) 13C 1H (J, Hz) 13C
1 2.41 t (7.9) 36.5 2.50 t (7.8) 30.4 2.49 t (7.9) 36.1 2.55 t (7.7) 30.4
2 1.79 m 33.0 1.76 m 31.9 1.81 m 33.2 1.79 m 31.8
3 2.52 t (7.7) 30.6 2.54 t (7.8) 30.7 2.54 t (7.7) 30.6 2.55 t (7.7) 30.6
1' 140.2 124.7 137.2 124.3
2' 6.18 s 108.7 152.2 6.64 d (2.0) 116.5 153.3
3' 151.3 6.58 s 99.4 147.3 6. 61 s 99.6
4' 134.7 147.2 147.0 149.1
5' 151.3 141.0 6.79 d (8.2) 112.9 144.1 6.60 dd (8.2, 6' 6.18 s 108.7 6.60 s 117.9 120.6 6.75 s 116.3 2.0) 1'' 123.4 123.7 123.5 123.6
2'' 144.7 144.7 144.7 144.7
3'' 134.9 134.9 135.0 134.9
4'' 147.8 147.8 147.8 147.8
5'' 6.38 d (8.4) 103.8 6.39 d (8.4) 103.8 6.39 d (8.4) 103.9 6.39 d (8.4) 103.9
6'' 6.50 d (8.4) 120.5 6.51 d (8.4) 120.4 6.50 d (8.5) 120.5 6.51 d (8.3) 120.4
2'-OMe 3.76 s 56.8 3.78 s 56.6
4'-OMe 3.75 s 60.8 3.83 s 57.0 3.80 s 56.6 3.82 s 56.8
5'-OMe 3.76 s 57.6
4''-OMe 3.78 s 56.5 3.80 s 56.6 3.80 s 56.6 3.80 s 56.9 a δ (ppm) 500 MHz for 1H and 125 MHz for 13C; multiplicities; J values (Hz) in parentheses.
3.2.5 Structure elucidation of bussealin E (3.5)
39 The positive ESI-MS of bussealin E (3.5) displayed a pseudomolecular ion peak at
+ 1 m/z 331.1181 [M + H] corresponding to the molecular formula C18H19O6. The H NMR spectrum in CDCl3 showed signals for a singlet aromatic proton at δH 6.70, two OH groups (δH 5.75 and 5.69), three OCH3 groups at δH 4.24, 4.24, and 4.01, and three
13 methylene groups as multiplets at δH 3.13, 3.12 and 2.17. The C NMR spectrum of 3.5 exhibited 18 signals, assigned to three methylene (δC 35.5, 28.7, 24.3), three OCH3 (δC
60.8, 60.8 and 61.7), and twelve aromatic carbons of two isolated aromatic rings.
Figure 3.3 Chemical structure of bussealin E (3.5) and 3.6
Seven of the aromatic carbons were oxygenated, based on their deshielded chemical shifts (Table 3.2). The ten degrees of unsaturation implied by the molecular formula
1 1 C18H18O6 required two additional rings. Interpretation of H- H COSY, HMQC, HMBC
OH COSY
HO O Me HMBC
O O O NOESY Me Me
Figure 3.4 COSY, HMBC and NOESY correlations of 3.5 and NOESY spectra allowed assignment of the locations of the functionalities present in
3.5. In the COSY spectrum, the three methylene groups were mutually coupled. The
40 assignment of a singlet aromatic proton was substantiated by the observation of HMBC correlations from H-1 (δH 6.70) to C-10 (δC 35.5), C-3b (δC 118.2), and two oxygenated aromatic carbons at C-2 (δC 146.5) and C-3 (δC 129.7). HMBC correlations from the signal at δH 5.69 to C-1 (δC 110.1), C-2 (δC 146.5) and the methoxylated carbon at C-3 (δC
129.7) were observed, substantiating the location of a hydroxy group at C-2. The other hydroxy group was assigned to position 7 based on the observation of HMBC correlations from the signal at δH 5.75 to the carbon signals at C-6 (δC 136.5), C-7 (δC
142.3) and C-7a (δC 115.0). In addition, the signal at δH 5.75 showed NOESY correlations to H-8 (δH 3.13) and 6-OMe (δH 4.01). These observations required that the remaining OCH3 group be placed at C-5. Furthermore, the HMBC correlations observed from H-10 (δH 3.12) to C-1 (δC 110.1), C-10a (δC 131.7), C-3b (δC 118.2), C-8 (δC 28.7) and C-9 (δC 24.3) confirmed the location of the cycloheptadiene ring. The above data confirmed the cycloheptadibenzofuran skeleton of 3.5. Assignments of the 13C NMR signals of C-3a, C-4a and C-4b were made by comparing the measured data with those calculated by ACD/ChemSketch version 11.01. The calculated shifts were in excellent agreement with the observed values, and were all within the standard deviation of the software (5 ppm), except for C-7a. Therefore, the structure of 3.5 was assigned as 9,10- dihydro-2,7-dihydroxy-3,5,6-trimethoxy-8H-cyclohepta[klm]dibenzofuran.
It is noteworthy that bussealin E is the first cycloheptadibenzofuran isolated from natural sources, and the cycloheptadibenzofuran skeleton is rare among synthetic compounds; the only simple synthetic compound with this ring system is 9,10-dihydro-1- methyl-8H-cyclohepta[klm]dibenzofuran (3.6) and its 8-keto derivative.6
41 Table 3.2 1H and 13C NMR Data for Bussealin E (3.5)a
1 a 13 a 13 b 13 c position H (J, Hz) C C C
1 6.70 s 110.1 110.0 6.59 s
2 146.5 149.1
3 129.7 131.3
3a 146.2 148.7
3b 118.2 113.3
4a 140.7 139.7
4b 120.6 117.4
5 135.6 137.0
6 136.5 137.9
7 142.3 145.1
7a 115.0 109.7
8 3.13 m 28.7 28.9 3.08 m
9 2.17 m 24.3 24.2 2.12 m
10 3.12 m 35.5 34.5 3.07 m
10a 131.7 128.6
3-OCH3 4.24 s 60.8 61.5 4.18 s
2-OH 5.69 s
5-OCH3 4.24 s 60.8 61.6 4.08 s
6-OCH3 4.01 s 61.7 61.0 3.90 s
7-OH 5.75 s 1 13 a. In CDCl3; δ (ppm) 600 MHz for H and 150 MHz for C; multiplicities; J values (Hz) in parentheses. b. Calculated using ACD/ChemSketch version 11.01. 1 c. In CD3OD; (ppm) 600 MHz for H; multiplicities; J values (Hz) in parentheses.
3.2.6 Possible biosynthesis of bussealin E (3.5)
The presence of diphenylpropanes in B. sakalava suggests that bussealin E is biosynthesized by oxidative coupling of an appropriate precursor diphenylpropane. This
42 could be followed by nucleophilic attack from a phenolate anion on a carbonyl group followed by dehydration to afford the new cycloheptadibenzofuran skeleton (3.5) as indicated in Scheme 3.1.
Scheme 3.1. Possible biosynthesis of cycloheptadibenzofuran 3.5 in B. sakalava
3.2.6 Antiproliferative activities of bussealins A-E (3.1-3.5)
The bioactivity of diphenylpropanes has been widely studied. The diphenylpropane broussonin A inhibited respiratory syncytial-virus (RSV) more effectively than the standard antiviral drug ribavirin,7 and its anti-aromatase activity has also been evaluated.8
Broussonin B moderately inhibited a chymotrypsin-like activity of the proteasome.9 The
43 anti-inflammatory,10-11 antifungal,12 antivascular,13 adipogenic,14 and anti-hCNT3
(human concentrative nucleoside transporter 3)15 activities of diphenylpropane analogues have also been reported. Since there have been no previous studies on the properties of diphenylpropanes on human ovarian cancer cells, we investigated the antiproliferative activity of diphenylpropanes 3.1-3.4 against the A2780 human ovarian cancer cell line.
Bussealins A-D (3.1-3.4) showed only weak antiproliferative activities, with IC50 values of 36, 24, 36, and 40 μM, respectively. Bussealin E (3.5), with a new chemical skeleton, was also tested against the A2780 cell line, but it also only exhibited weak activity with an IC50 value of 45 μM. The new skeleton of bussealin E thus does not appear to confer any novel antiproliferative activity beyond that which is normal for diphenylpropanes.
3.3 Experimental Section
3.3.1 General experimental procedures
UV and IR spectra were measured on a Shimadzu UV-1201 spectrophotometer and a
MIDAC M-series FTIR spectrophotometer, respectively. NMR spectra were recorded in
CD3OD or CDCl3 on either JEOL Eclipse 500 or Bruker Avance 600 spectrometers. The chemical shifts are given in δ (ppm) and coupling constants (J) are reported in Hz. Mass spectra were obtained on an Agilent 6220 LC-TOF-MS. HPLC was performed on a
Shimadzu LC-10AT instrument with a semi-preparative C18 Varian Dynamax column (5
m, 250 x 10 mm).
3.3.2 Antiproliferative bioassays
44 The A2780 ovarian cancer cell line assay was performed at Virginia Polytechnic
Institute and State University as previously reported,16 except that the samples were added in 1L 100% DMSO per well instead of 20 L of 1:1 DMSO:H2O. The A2780 cell line is a drug-sensitive human ovarian cancer cell line.17
3.3.3 Plant Material
A sample of root of Bussea sakalava Du Puy & R. Rabev. (Fabaceae) was collected on January 25, 2007, near Ambolobozobe, Madagascar at coordinates 12°31'26''S
49°31'29''E, at an elevation of 20 m. Its assigned collection number is Rakotonandrasana et al. 1079. The genus Bussea Harms is a small genus including 7 species (5 from
Tropical Africa and 2 from Madagascar). B. sakalava is endemic to deciduous forest from western to northern Madagascar. The hard wood of this species is used in construction and as firewood.18 Voucher specimens have been deposited at the Parc
Botanique and Zoologique de Tsimbazaza (TAN) and at the Centre National d'Application des Recherches Pharmaceutiques (CNARP) in Antananarivo, Madagascar; the Missouri Botanical Garden in St. Louis, Missouri (MO); and the Muséum National d'Histoire Naturelle in Paris, France (P).
3.3.4 Extraction and Isolation
Dried roots of B. sakalava (275 g) were ground in a hammer mill, then extracted with ethanol by percolation for 24 hours at room temperature to give the crude extract MG
4273 (14.4 g), of which 3.0 g was shipped to Virginia Polytechnic Institute and State
University (VPISU) for bioassay-guided isolation. The fractionation tree is shown in
45 Scheme 3.2. Sample MG 4273 (IC50 9.6 g/mL, 2.1 g) was suspended in aqueous MeOH
(MeOH-H2O, 9:1, 100 mL) and extracted with hexane (3 x 100 mL portions). The aqueous layer was then diluted to 60% MeOH (v/v) with H2O and extracted with CH2Cl2
(3 x 150 mL portions). The hexane extract was evaporated in vacuo to leave 227 mg with an IC50 value of 19 g/mL. 102.9 mg of residue from the CH2Cl2 extract had an IC50 of
Scheme 3.2 Separation of ethanol extract of Bussea sakalava
10 g/mL. The aqueous MeOH extract (1.7 g) was inactive. The CH2Cl2 extract was selected for fractionation using an SPE cartridge over C18, and two fractions were collected. Fractions I and II (70.2 mg and 26.8 mg) had IC50 values of 8.6 and 15 g/mL,
46 respectively. Fraction I was separated by C18 HPLC (65% MeOH-H2O), and compounds
3.1 (3.3 mg tR 12.5 min), 3.2 ( 1.7 mg tR 18.6 min), 3.3 (2.0 mg tR 22.0 min), 3.4 (1.1 mg tR 29.5 min) and 3.5 (1.1 mg tR 26.5 min) were isolated. HPLC chromatogram of Fraction
I is shown in Scheme 3.3.
Scheme 3.3 HPLC Chromatogram of fraction II
3',5',2'',3''-tetrahydroxy-4',4''-dimethoxy-1,3-diphenyl-propane (3.1)
Off-white solid; UV (MeOH) λmax nm (log ε) 218 (4.40), 267 (3.69), 294 (3.52); IR
-1 1 13 max cm : 3367, 1648, 1450, 1115, 1024. H NMR (500 MHz, CD3OD) and C NMR
+ (125 MHz, CD3OD), see Table 3.1; ESI-MS m/z 321.1338 [M + H] (calcd for C17H21O6,
321.1338).
5',2'',3''-trihydroxy-2'',4',4''-trimethoxy-1,3-diphenyl-propane (3.2)
Off-white solid; UV (MeOH) λmax nm (log ε) 214 (4.25), 229 (sh) (4.10), 290 (3.59)
-1 1 13 nm; IR max cm : 3332, 1599, 1444, 1095, 1032; H NMR (500 MHz, CD3OD) and C
+ NMR (125 MHz, CD3OD), see Table 3.1; ESI-MS m/z 335.1512 [M + H] (calcd for
C18H23O6, 335.1495).
47 3',2'',3''-Trihydroxy-4',4''-dimethoxy-1,3-diphenyl-propane (3.3)
Off-white solid; UV (MeOH) λmax nm (log ε) 208 (4.15), 267 (3.54), 289 (3.47) nm;
-1 1 13 IR max cm : 3338, 1656, 1450, 1115, 1024; H NMR (500 MHz, CD3OD) and C NMR
+ (125 MHz, CD3OD), see Table 3.1; ESI-MS m/z 305.1384 [M + H] (calcd for C17H21O5,
305.1389).
3',2'',3''-Trihydroxy-4',4''-dimethoxy-1,3-diphenyl-propane (3.4)
Off-white solid; UV (MeOH) λmax nm (log ε) 210 (4.21), 229 (sh) (4.01), 289 (3.48)
-1 1 13 nm; IR max cm : 3350, 1602, 1450, 1115, 1026; H NMR (500 MHz, CD3OD) and C
+ NMR (125 MHz, CD3OD), see Table 3.1; ESI-MS m/z 349.1648 [M + H] (calcd for
C19H25O6, 349.1651).
9,10-Dihydro-2,7-dihydroxy-3,5,6-trimethoxy-8H-cyclohepta[klm]dibenzofuran (3.5)
Off-white solid; UV (MeOH) λmax nm (log ε) 218 (4.25), 270 (3.78), 294 (3.73), 316
-1 1 (3.47)) nm; IR max cm : 3332, 1567, 1449, 1115, 1024; H NMR (600 MHz, CD3OD
13 and CDCl3) and C NMR (150 MHz, CD3OD), see Table 3.2; ESI-MS m/z 331.1181 [M
+ + H] (calcd for C18H19O6, 331.1182).
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14. Youn, U. J.; Lee, Y. S.; Jeong, H.; Lee, J.; Nam, J. W.; Lee, Y. J.; Hwang, E. S.; Lee,
J. H.; Lee, D.; Kang, S. S.; Seo, E. K., Identification of Antiadipogenic Constituents
of the Rhizomes of Anemarrhena asphodeloides. J. Nat. Prod. 2009, 72, 1895-1898.
15. Gupte, A.; Buolamwini, J. K., Synthesis and biological evaluation of phloridzin
analogs as human concentrative nucleoside transporter 3 (hCNT3) inhibitors. Bioorg.
Med. Chem. Lett. 2009, 19, 917-921.
16. Cao, S.; Brodie, P. J.; Miller, J. S.; Randrianaivo, R.; Ratovoson, F.; Birkinshaw, C.;
Andriantsiferana, R.; Rasamison, V. E.; Kingston, D. G. I., Antiproliferative
50 Xanthones of Terminalia calcicola from the Madagascar Rain Forest1. J. Nat. Prod.
2007, 70, 679-681.
17. Louie, K. G.; Behrens, B. C.; Kinsella, T. J.; Hamilton, T. C.; Grotzinger, K. R.;
McKoy, W. M.; Winker, M. A.; Ozols, R. F., Radiation survival parameters of
antineoplastic drug-sensitive and drug-resistant human ovarian-cancer cell-lines and
their modification by buthionine sulfoximine Cancer Res. 1985, 45, 2110-2115.
18. Du Puy, D. J.; Labat, J.-N.; Rabevohitra, R.; Villiers, J.-F.; Bosser, J.; Moat, J., The
Leguminosae of Madagascar. Royal Botanical Gardens: Kew 2002, 22-24.
IV. Cardenolides of Leptadenia madagascariensis from the Madagascar dry forest
This chapter is a slightly expanded version of a published article.2 Attributions from co-authors of the article are described as follows in the order of the names listed. The author of this dissertation (Mr. Ende Pan) conducted isolation and structure elucidation of
51 the titled compounds, and drafted the manuscript. Dr. Liva Harinantanaina was a mentor for this work, and in particular, he provided invaluable advice and hints for structure elucidation of those compounds, and he also proofread the manuscript before submission.
Ms. Peggy Brodie performed the A2780 bioassay on the isolated fractions and compounds. Dr. Martin Callmander and Dr. Stephan Rakotonandrasana from Missouri
Botanical Garden did the plant collections and identification. Dr. Etienne Rakotobe, and
Dr. Vincent E. Rasamison from Madagascar carried out the initial plant extraction. Dr.
Karen TenDyke, Dr. Yongchun Shen, and Dr. Edward M. Suh from Eisai Inc. performed the H460 bioassay on the isolated compounds. Dr. David G. I. Kingston was a mentor for this work and the corresponding author for the published article. He provided critical suggestions for this work and crucial revisions to the manuscript.
4. 1 Introduction
In our continuing search for biologically active natural products from tropical rainforests as part of an International Cooperative Biodiversity Group (ICBG) program,3-4 we obtained an EtOH extract from the roots of the plant Leptadenia madagascariensis
Decne. (Apocynaceae) from Madagascar. The extract exhibited good antiproliferative activity against the A2780 human ovarian cancer cell line, with an IC50 value of 10
g/mL. On the basis of this activity and the absence of any previous phytochemical studies on this species, the extract was selected for fractionation to isolate its active components by bioassay-guided fractionation.
4.1.1 Previous Investigations of Leptadenia
52 There are about twenty species in the genus Leptadenia, some of which are used in traditional medicine in Africa and India.5-7 Previous phytochemical investigations reported the presence of flavonoidsError! Bookmark not defined.,8-9 terpenoids6, 8, 10-11 polyoxypregnane esters,5, 12
Figure 4.1 Compounds from the genus Leptadenia pregnane glycosides,13-14 cardiac glycosides15 and alkaloids16 in these species. The medicinal use of plants containing cardiac glycosides was recorded as early as 1500 years ago. As an important class of natural products, cardiac glycosides are widely used for treating cardiac failure,17 and their cardiac activities and cytotoxicities are well known.Error! Bookmark not defined.,18-19 What is less well known is the fact that they
53 are also beginning to find use in cancer chemotherapy, and the first generation of anticancer cardiac glycosides is in clinical trials.20-21
4.2 Results and Discussion
The EtOH extract of the stems and leaves of L. madagascariensis was subjected to liquid-liquid partitioning to give active dichloromethane and MeOH fractions with IC50 values in the A2780 assay of 0.28 and 2.4 µg/mL, respectively. Fractionation by C18 open column and High Performance Liquid Chromatography (HPLC) on the MeOH fraction yielded two new cardenolides named madagascarensilide A (4.1) and madagascarensilide
B (4.2) (Figure 4.2). Similar purification of the CH2Cl2 fraction yielded the two additional new compounds madagascarensilides C and D (4.3 and 4.4, Figure 3). Herein we report the structural elucidation and the antiproliferative properties of the four isolates.
4.2.1 Structure elucidation of madagascarensilide A (4.1)
Madagascarensilide A (4.1) was obtained as a white solid. Its positive ion HRESIMS revealed a pseudomolecular ion peak at m/z 849.4255 [M+Na]+, corresponding to a
1 molecular formula of C42H66O16 for 4.1. Its H NMR spectrum in CD3OD showed signals at δH 5.04 dd (J = 18.6, 1.8 Hz), 4.92 dd (J = 18.6,1.8 Hz), and 5.90 s, characteristic of an
α, β-unsaturated lactone (Table 4.1). In addition, three anomeric proton signals were observed at δH 4.91 dd (J = 9.5, 1.8 Hz), 4.57 d (J = 7.7 Hz) and 4.37 d (J = 7.7 Hz). The
13C NMR spectrum contained 42 signals, which included signals for one methoxyl, four methyls, 12 methylenes (including one oxymethylene), 20 methines (including 15
54 oxymethines and one olefinic carbon), and five quaternary carbons (including one oxyquaternary carbon, one olefinic carbon and one carbonyl carbon), as indicated by an
HMQC spectrum (Table 4.1). The above data suggested that 4.1 is a cardiac glycoside with three sugar moieties.
Figure 4.2 Chemical structures of madagascarensilides A (4.1) and B (4.2).
In the aglycone of 4.1, two spin systems CH2-CH2-CH-CH2-CH-CH2-CH2-CH-CH-
CH2-CH2 (H2-1 through H2-2, H-3, H2-4, H-5, H2-6, H2-7, H-8, H-9 and H2-11 to H2-12) in rings A, B and C, and CH2-CH2-CH (H2-15 through H2-16 to H-17) in ring D (Figure
4.2) were identified in the COSY and TOCSY spectra. The connectivities of rings A, B,
C and D were assigned based on the interpretation of the HMBC spectrum. Long-range correlations from H3-19 to C-1, C-5, C-9 and C-10, and from H2-1 to C-9 indicated the connectivity of rings A and B. The relationship between rings C and D was established by the observation of correlations from H3-18 to C-12, C-13, the oxygenated quaternary carbon at C-14 and C-17, as well as those observed from H2-12 to C-17, and H2-15 to C-
8. Moreover, the α, β-unsaturated lactone was deduced to be connected to C-17 by the
HMBC correlation from H-17 to C-20, C21 and C22. The Rotating frame Overhauser
55 Effect SpectroscopY (ROESY) correlation between H3-19 and H-5 indicated that rings A and B are cis fused, while the trans orientation of H-8 and H-9 was deduced from the presence of correlations between H3-18 and H3-19 to H-8 (Figure 4.3). The correlations of H3-18 to C-14-OH, H-21, and H-22 in the ROESY spectrum in deuterated pyridine indicated cis fused C and D rings and the β-orientation of the -lactone at C-17. These data, together with a comparison of the 13C NMR data of 4.1 with those of the aglycone of coroloside and similar digitoxigenin glycosides, established the aglycone of 4.1 as digitoxigenin.22
The presence of three sugar units in 4.1 was indicated by the presence of three anomeric proton signals at δH 4.91, 4.57 and 4.37. Their spin systems were determined by
COSY and TOCSY correlations: H-1'-H2-2'-H-3'-H-4'-H-5'-H3-6', H-1''-H-2''-H-3''-H-4''-
H-5''-H3-6'', and H-1'''-H-2'''-H-3'''-H-4'''-H-5'''-H2-6'''. In addition, HMBC correlations from H-1' to C-3, H-1'' to C-4', and H-1''' to C-4'' built up the connectivity of the sugar units from C-1' to C-3, C-1'' to C-4' and C-1''' to C-4''. The relative conformations of the sugar moieties were determined by the coupling constants of the sugar protons and by analysis of the ROESY data. In the 1H NMR spectrum of 4.1 in deuterated pyridine, the coupling constants observed for H-1' (d, J = 9.5, 1.8) and for H-4' (dd, J = 9.7, 2.7) as well as the clear ROESY correlation between H-1' and H-5' indicated that H-1', H-4' and
H-5' are all axial, and so H-3' must be equatorial based on its 2.7 Hz coupling constant with H-4'.
56
Figure 4.3 a) Key COSY (bold) and HMBC (arrows) correlations for 4.1. b) Key ROESY correlations for 4.1.
The HMBC correlation between the methoxy protons at 3.69 ppm and C-3'' (δC 85.6) placed the methoxy group at C-3''. In the same manner, the coupling constants observed for H-1'' (d, J = 7.7 Hz), H-3'' (dd, J = 9.7, 2.9 Hz) and the ROESY correlation of H-5'' with H-1'' indicated that H-1'', H-2'', H-3'', H-5'' are axial, while H-4'' is equatorial. The coupling constants observed for the third sugar unit for H-1''' (d, J = 7.8 Hz) and H-2''' (t,
J = 7.8 Hz), with ROESY correlations from H-1''' to H-3''' and H-5''', as well as from H-
2''' to H-4''', led to the conclusion that all the protons in this sugar unit must be axial,
57 indicating it to be glucopyranose. Therefore, the structure of 4.1 was determined to be digitoxigenin 3-O-β-glucopyranosyl-(1->4)-O-β-digitalopyranosyl-(1->4)-O-β- digitoxopyranoside. The absolute stereochemistry of the glucose unit was assigned as D since L-glucose has never been observed in cardenolides. The digitoxose and digitalose units were also assigned as D-sugars based on their occurrence in other cardenolides in the D-form.Error! Bookmark not defined.,23
4.2.2 Structure elucidation of madagascarensilide B (4.2)
Madagascarensilide B (4.2) was obtained as a white solid. Its positive ion HRESIMS revealed a pseudomolecular ion peak at m/z 687.3728, corresponding to a molecular
1 formula of C36H56O11 for 4.2. The H NMR data of 2 in CD3OD were very similar to those of 4.1, and thus its structure was indicated to be a cardiac glycoside with two sugar units. The 1H and 13C NMR data arising from the aglycone and the sugar moiety attached at C-3 of 4.2 were essentially superposable with those of 4.1. In addition, the spin system
H-1''-H-2''-H-3''-H-4''-H-5''-H3-6'' was identified by analysis of the COSY data (Table
4.1). The HMBC correlation observed between the methoxy protons and C-3'' indicated that the second sugar moiety shares the same planar structure as the second sugar of 4.1.
The coupling constants observed at H-1'' (d, J = 7.7 Hz), H-2'' (dd, J = 9.7, 7.7 Hz), H-3''
(dd, J = 9.7, 3.1 Hz) and H-5'' (brq, J = 6.4 Hz), together with the ROESY correlation between H-1'' and H-5'' indicated that H-1'', H-2'', H-3'' and H-5'' are axial while H-4'' is equatorial. Thus the structure of 4.2 was assigned as digitoxigenin 3-O-β- digitalopyranosyl-(1->4)-O-β-digitoxopyranoside.
4.2.3 Structure elucidation of madagascarensilide C (4.3)
58 Madagascarensilide C (4.3) was obtained as a white solid. Its positive ion HRESIMS revealed a pseudomolecular ion peak at m/z 845.4315 [M+Na]+ corresponding to a
1 13 molecular formula of C43H66O15 for 4.3. The H and C NMR spectra in deuterated pyridine indicated compound 4.3 to be a cardenolide with three sugar units, with signals for three anomeric protons at δH 5.39, 5.17, and 4.69 ppm and one aldehyde at δH 10.42
(Table 4.1). In the aglycone of 4.3, three spin systems: CH2-CH2-CH-CH2 (H2-1 through
H2-2, H-3 to H2-4) for ring A; CH2-CH2-CH-CH-CH2-CH2 (H2-6 through H2-7, H-8, H-
9, H2-11 to H2-12) in rings B and C; and CH2-CH2-CH (H2-15 through H2-16 to H-17) in ring D were identified by COSY and TOCSY spectra. The connectivities of rings A, B, C and D were assigned based on an analysis of HMBC data. The long-range correlations arising from H-19 at δH 10.42 to C-1 and H-9 at δH 1.78 to C-19 at δC 208.9, as well as the correlations from Ha-4 and Ha-6 to C-10, and from the hydroxyl group signal at C-5 to C-
4 and C-6, indicate the connectivity of rings A and B. Meanwhile, the relationship between rings C and D was established by the observation of correlations from H3-18 to
C-12, C-13, C-14 and C-17, and from the hydroxy group at C-14 to C-8, C-13, C-14 and
C-15. The α,β-unsaturated -lactone was determined to be at C-17 by the HMBC correlation from H-17 to C-20, C21 and C22. Moreover the assigned 13C NMR chemical shifts of the aglycone of 4.3 in CD3OD (Table 4.1) are very similar to those of strophanthidin.24 From the above data, the planar structure of the aglycone of 3 was deduced to be strophanthidin.
The spin systems H-1'-H2-2'-H-3'-H-4'-H-5'-H3-6', H-1''-H2-2''-H-3''-H-4''-H-5''-H3-6'', and H-1'''-H2-2'''-H-3'''-H-4'''-H-5'''-H3-6''' of the sugar units were assigned by COSY and
TOCSY correlations. Long-range correlations from H-1' to C-3, H-1'' to C-4', and H-1'''
59 to C-4'' established the connectivity of the sugar units as depicted in Figure 4.4. The methoxy groups at C-3'' and C-3''' were substantiated by observation of HMBC correlations in deuterated pyridine between the methoxy signals (δH 3.51 and 3.40, each singlet) and the two carbon signals at δC 78.1 and 79.3 (C-3'' and C-3''', respectively).
The relative conformations of the sugar moieties were determined by analysis of the
ROESY data of 4.3 and the coupling constants of the sugar protons. The values of the coupling constants of H-1' (J = 9.6, 1.9 Hz) and H-4' (J = 9.7, 3.0 Hz) and the clear
ROESY correlation between H-1' and H-5' indicated that H-1', H-4' and H-5' are axial, while H-3' is equatorial. The coupling constants of H-1'' (dd, J = 9.7, 1.8 Hz) and H-4''
(dd, J = 9.7, 2.7 Hz) and the ROESY correlation between H-5'' and H-1'' suggested that
H-1'', H-4'', and H-5'' are axial, while H-3'' is equatorial. Similarly, the coupling constants of H-1''' (dd, J = 9.7, 2.1 Hz), H-3''' (ddd, J = 12.1, 4.7, 2.9 Hz) and H-5''' (qd, J = 6.4, 1.3
Hz), and the ROESY correlation between H-1''' and H-5''' indicate that H-1''', H-3''' and
H-5''' are axial and H-4''' is equatorial. Therefore, the structure of 4.3 was determined as strophanthidin 3-O-β-diginopyranosyl-(1->4)-O-β-cymaropyranosyl-(1->4)-O-β- digitoxopyranoside.
60 Table 4.1 1H and 13C NMR chemical shifts of madagascarensilides A (4.1), B (4.2), C (4.3) and D (4.4) a position 4.1b 4.1c 4.2b 4.3c 4.3b 4.4b 1H (J, Hz) 13C 1H (J, Hz) 13C 1H (J, Hz) 13C 1H (J, Hz) 13C 1H (J, Hz) 13C 1H (J, Hz) 13C
Aglycone 1 1.46 m 31.0 30.9 31.0 2.59m, 1.91m 18.9 1.72 m, 1.60m 18.9 18.9 2 1.63 m 27.5 27.5 28.1 2.19m, 1.68m 26.0 1.94m, 1.61m 25.9 25.9 3 4.02 m 74.5 4.27 m 73.6 4.26 m 74.5 4.33 m 75.3 4.15 m 76.3 4.15 m 76.3 4 1.83 m, 1.46 m 31.4 31.2 31.4 2.19m, 1.73m 36.2 2.17m, 1.62m 36.8 36.8 5 1.66 m 38.0 37.4 38.0 OH 4.89s 74.1 75.2 75.2 6 1.88 m, 1.26 m 27.9 27.5 27.5 2.34m, 1.82m 37.3 2.17m, 1.62m 37.2 37.2 7 1.78 m, 1.25 m 22.4 22.0 22.4 2.33 m, 1.46m 25.2 2.12m, 1.32m 25.2 25.2 8 1.63 m 42.7 42.3 42.7 2.31 m 42.3 1.94 m 42.6 42.6 9 1.73 m 36.9 36.3 36.9 1.78m 39.9 1.66m 40.4 40.4 10 36.3 35.9 36.3 56.1 56.1 56.1 11 1.43 m, 1.24 m 22.6 22.4 22.6 1.59m, 1.40m 23.0 1.56m, 1.51m 23.3 23.3 12 1.51 m 41.0 40.3 41.0 1.45 m, 1.35m 39.9 1.49 m, 143m 40.5 40.5 13 51.1 50.5 51.1 50.2 50.7 50.7 14 86.5 85.0 86.5 -OH, 5.66 84.8 85.9 85.9 15 2.18, 1.73 33.4 33.6 33.4 2.08m, 1.86m 32.5 2.17 m, 1.72 m 32.4 32.4 16 2.18, 1.88 28.1 27.7 27.9 2.10m, 2.02m 27.6 2.19 m, 2.14 m 27.9 27.9 17 2.83 m 52.1 2.79 m 51.9 2.83 m 52.1 2.79 m 51.5 2.82 m 51.7 2.82 m 51.8 18 0.88 s 16.4 0.89 s 16.6 0.88 s 16.4 1.01 16.4 0.85 s 16.2 0.85 s 16.2 19 0.94 s 24.3 1.02 s 24.3 0.95 s 24.3 10.42 208.9 10.05 s 209.9 10.05 s 209.9 20 178.5 176.4 178.5 176.1 178.2 178.2 5.04 dd (18.6, 1.8) 5.34 dd (18.2, 1.4) 5.03 dd (18.4, 1.5) 5.31 dd (18.2, 1.7) 5.03 dd (18.5, 1.7) 5.03 dd (18.5, 1.7) 21 75.4 74.1 75.4 74.4 75.3 75.3 4.92 dd (18.6,1.8) 5.06 dd (18.2,1.4) 4.92 dd (18.4,1.5) 5.05 dd (18.2, 1.7) 4.91dd (18.5, 1.7) 4.91dd (18.5, 1.7) 22 5.90 s 117.8 6.15 s 118.1 5.90 s 117.8 6.14 118.2 5.90 117.9 5.90 117.9 23 177.3 174.9 177.3 174.9 177.2 177.2
61 Sugar I 1' 4.91 dd (9.5, 1.8) 96.8 5.44 dd (9.5, 1.8) 96.9 4.91 dd (9.4, 1.5) 96.8 5.39 dd (9.6, 1.9) 98.0 4.91 dd (8.9, 1.9) 98.3 4.91 dd (8.9, 1.9) 98.3 2' 1.95 m, 1.73 m 39.0 2.43 m, 2.11 m 39.9 1.95 m, 1.73 m 38.9 2.34 m, 1.93 m 39.1 1.98 m, 1.71 m 38.7 1.98 m, 1.71 m 38.7 4.61 m 3' 4.24 m 68.7 4.71 m 68.1 4.26 m 68.7 67.8 4.24 m 68.3 4.24 m 68.3 -OH, 5.52 4' 3.23 m 84.3 3.67 dd (9.7, 2.7) 84.7 3.23 dd (9.5, 2.9) 84.1 3.50 dd (9.7, 3.0) 83.2 3.23 dd (9.5, 2.9) 83.5 3.23 m 83.5 5' 3.85 m 69.7 4.37 m 69.3 3.85 m 69.7 4.26 m 69.2 3.80 m 69.6 3.80 m 69.6 6' 1.31 d (6.5) 18.6 1.67 d (6.2). 19.2 1.29 d (6.2) 18.5 1.41d (6.3) 18.9 1.21 d (6.2) 18.4 1.21 d (6.1) 18.4 Sugar II 1'' 4.37 d (7.7) 106.2 4.77 d (7.7) 106.7 4.34 (7.7) 106.2 5.17 dd ( 9.7, 1.8) 100.1 4.84 m 100.7 4.84 m 100.6 2'' 3.66 m 71.4 4.42 m 71.7 3.55 dd (9.7, 7.7) 71.4 2.28 m, 1.76m 36.9 2.15 m, 1.62 m 36.2 2.15 m, 1.62 m 35.8 3'' 3.25 m 85.3 3.57 dd (9.7, 2.9) 85.6 3.12 dd (9.7, 3.1) 84.4 4.07 m 78.1 3.87 m 78.5 3.87 m 78.2 4'' 4.16 m 76.3 4.28 m 77.8 3.85 m 68.6 3.48 dd (9.7, 2.7) 83.3 3.30 m 83.5 3.30 m 83.7 5'' 3.66 m 71.8 3.75 m 71.0 3.63 bq (6.4) 71.6 4.20 m 69.5 3.86 m 70.1 3.86 m 70.0 6'' 1.29 d (6.5) 17.4 1.55 d (6.4) 17.9 1.27 d (6.4) 17.0 1.34 d 6.3 18.9 1.22 d (6.2) 18.6 1.22 d (6.2) 18.6 Sugar III 1''' 4.57 d (7.7) 103.9 5.10 d (7.8) 106.4 4.69 dd ( 9.7, 2.1) 103.1 4.55 dd (9.7, 2.0) 103.4 4.57 dd (9.7, 1.9) 103.4 2''' 3.22 m 76.0 4.02 t (7.8) 76.6 2.17 m, 2.29 m 33.1 1.93 m, 1.65 m 32.9 2.00 m, 1.81m 33.2 3''' 3.37 m 78.2 4.26 m 79.1 3.41 ddd (12.1, 4.7, 2.9) 79.3 3.35 m 79.2 3.47 m 80.6 3.91 m 4''' 3.26 m 71.8 4.25 m 72.2 67.1 3.67 m 67.7 3.99 m 74.6 -OH 6.00 5''' 3.26 m 78.0 3.98 m 78.9 3.56 qd (6.4, 1.3) 71.9 3.49 qd (6.6, 1.3) 72.0 3.53 m 71.8 6''' 3.88 m, 3.65 m 63.0 4.59 m, 4.38 m 63.4 1.55 d (6.4) 17.9 1.28 d (6.6) 17.2 1.30 d (6.4) 17.7 Sugar IV 1'''' 4.56 d (7.7) 104.6 2'''' 3.22 m 76.0 3'''' 3.36 m 78.2 4'''' 3.27 m 71.8 5'''' 3.25 m 78.0 6'''' 3.86m, 3.64m 63.0
3''-OCH3 3.52 s 58.8 3.69 s 59.5 3.46 57.3 3.51 s 59.1 3.44 s 58.4 3.42 s 58.0
3'''-OCH3 3.40 s 55.7 3.38 s 55.8 3.41 s 56.6 a δ (ppm) 500 MHz for 1H and 125 MHz for 13C; multiplicities; J values (Hz) in parentheses. b In CD3OD c In deuterated pyridine
62
Figure 4.4 Chemical structures of madagascarensilides C (4.3) and D (4.4)
4.2.4 Structure elucidation of madagascarensilide A (4.4)
Madagascarensilide D (4.4) was obtained as a white solid. Its positive ion HRESIMS revealed a pseudomolecular ion peak at m/z 1007.4844 [M+Na]+ corresponding to a
1 13 molecular formula of C49H76O20 for 4.4. The H and C NMR spectroscopic data of 4.4 were very similar to those of 4.3, suggesting that 4.4 is a cardenolide derivative with four sugar units (Table 4.1). Inspection of the carbon chemical shifts of 4.4 revealed a close similarity to those of 3 except for the downfield shifts of the signals arising from C-3'''
(+1.4) and C-4''' (+6.9) and the upfield shift of the signal of C-5''' (-0.2). These data indicated that the additional sugar unit in 4.4 is linked to the third sugar, and the position of attachment of this fourth sugar unit was confirmed to be at C-4''' due to observation of a clear HMBC correlation between H-1'''' and C-4'''. The 13C NMR data of the fourth sugar unit (δC 104.6, 76.0, 78.2, 71.8, 78.0, 63.0) were very similar to the those of the terminal β-glucopyranosyl unit of the tetrasaccharide adoligose B (β-Glc-β-Dgn-β-Cym-
β-Cym),25 indicating the sugar moieties of 4.4 to be β-Glc-β-Dgn-β-Cym-β-Dgx.
Compound 4.4 is thus strophanthidin 3-O-β-glucopyranosyl-(l->4)-O-β-diginopyranosyl-
(1->4)-O-β-cymaropyranosyl-(1->4)-O-β-digitoxopyranoside.
63
Figure 4.5 a) Key COSY (bold) and HMBC (arrows) correlations for 4.3.
b) Key ROESY correlations for 4.3.
4.2.5 Antiproliferative activities of madagascarensilide A-D (4.1-4.4)
Madagascarensilide A (4.1), B (4.2), C (4.3) and D (4.4) were tested for antiproliferative activity against the A2780 human ovarian cancer cell line. Compounds
4.1 and 4.3 were the most potent, having an IC50 value of 0.18 and 0.17 M, while compounds 4.2 and 4.4 were slightly less potent, with IC50 values of 0.21 and 0.29 M, respectively. It appears that the aldehyde group at position 10 between rings A and B on the aglycone does not significantly affect the activity of cardenolides against A2780 cells.
64 Cardenolides 4.1-4.4 were also evaluated in the H460 human lung cancer cell line.
Madagascarensilide A (4.1) showed strong activity with a IC50 value of 0.16 M.
Madagascarensilide B (4.2), C (4.3) and D (4.4) were also active with IC50 values of 0.68,
0.37, and 0.48 M, respectively.
4.3 Experimental Section
4.3.1 General experimental procedures
Optical rotations were recorded on a JASCO P-2000 polarimeter. UV and IR spectra were measured on a Shimadzu UV-1201 spectrophotometer and a MIDAC M-series
FTIR spectrophotometer, respectively. NMR spectra were obtained in CD3OD or deuterated pyridine on either JEOL Eclipse 500 or Bruker Avance 600 spectrometers.
The chemical shifts are given in δ (ppm) and coupling constants (J) are reported in Hz.
Mass spectra were obtained on an Agilent 6220 TOF Mass Spectrometer. HPLC was performed on a Shimadzu LC-10AT instrument with a semi-preparative C18 Varian
Dynamax column (5 m, 250 x 10 mm).
4.3.2 Antiproliferative bioassays
The A2780 ovarian cancer cell line assay was performed at Virginia Polytechnic
Institute and State University as previously reported,26 except that the samples were added in 1 L 100% DMSO per well instead of 20 L of 1:1 DMSO:H2O. The A2780 cell line is a drug-sensitive human ovarian cancer cell line.27 Assays against the NCI-
H460 lung cancer cell line were carried out at Eisai, Inc., as previously described for similar cell lines.28
65
4.3.3 Plant material
A sample of the roots of Leptadenia madagascariensis Decne. (Apocynaceae) was collected in 2007 2 km west of the village of Ambolobozobe, Madagascar, in degraded dry forest. Collection coordinates were 12° 31' 26'' S, 49° 31' 29'' E, and elevation 20 m.
Voucher specimens have been deposited at the Parc Botanique and Zoologique de
Tsimbazaza (TAN) and at the Centre National d'Application des Recherches
Pharmaceutiques (CNARP) in Antananarivo, Madagascar; the Missouri Botanical Garden in St. Louis, Missouri (MO); and the Muséum National d'Histoire Naturelle in Paris,
France (P), voucher number SR1092.
4.3.4 Extraction and Isolation
Dried roots of Leptadenia madagascariensis (275 g) were ground in a hammer mill, then extracted with EtOH by percolation for 24 hours at room temperature to give the crude extract MG 4294 (12.7 g), of which 3.34 g was shipped to Virginia Polytechnic
Institute and State University (VPISU) for bioassay guided isolation. The fractionation tree is shown in Scheme 4.1.The extract MG 4294 (IC50 3.6 g/mL, 2.0 g) was suspended in aqueous MeOH (MeOH-H2O, 9:1, 100 mL) and extracted with hexane (3 x 100 mL portions). The aqueous layer was then diluted to 60% MeOH (v/v) with H2O and extracted with CH2Cl2 (3 x 150 mL portions). The hexane extract was evaporated in vacuo to leave 352 mg with an IC50 value of 15 g/mL. The residue from the CH2Cl2 extract (223 mg) had an IC50 of 0.28 g/mL and the aqueous MeOH extract (1.22 g) had an IC50 of 2.4 g/mL. Fractionation of the aqueous MeOH extract by C18 open column
66 gave the four fractions I - IV (987.6, 70.4, 11.9 and 5.8 mg), with IC50 values of 10, 0.14,
16 and 14 g/mL, respectively.
Scheme 4.1 Separation of ethanol extract of Leptadenia madagascariensis
The most active fraction (fr-II) was separated further by C18 HPLC (solvent system:
MeOH-H2O 70:30), and compounds 4.1 (2.8 mg, tR 15.5 min) and 4.2 (0.7 mg, tR 21.4 min) were isolated. Fractionation of the CH2Cl2 extract on a C18 open column gave the
67 five fractions A - E (11.5, 67.3, 47.8, 44.6, and 8.8 mg) with IC50 values: >20, 0.11, 0.12,
0.97, and 6.9 g/mL, respectively. Fraction B was selected for further purification by
C18-HPLC (solvent system: gradient from MeOH: H2O 60:40 to 70:30 for 40 min) to afford compounds 4.3 (1.5 mg, tR 38.7 min) and 4.4 (5.0 mg, tR 28.0 min).
Digitoxigenin 3-O-β-glucopyranosyl-(1->4)-O-β-digitalopyranosyl(1->4)-O-β- digitoxopyranoside (4.1, madagascarensilide A)
23 Compound 4.1 was a white solid; [α]D +11 (c 0.28, MeOH); UV (MeOH) λmax nm
-1 -1 1 (log ε) 215 (4.1); IR max cm : 3396, 2934, 1739, 1449, 1372, 1073 cm . H NMR (500
13 MHz, CD3OD, d-pyridine) and C NMR (125 MHz, CD3OD, d-pyridine), see Table 4.1;
+ HRESI-MS m/z 849.4255 [M+Na] (calcd for C42H66NaO16 849.4249).
Digitoxigenin 3-O-β-digitalopyranosyl-(1->4)-O-β-digitoxopyranoside (4.2, madagascarensilide B)
23 Compound 4.2 was a white solid; [α]D +16 (c 0.07, MeOH); UV (MeOH) λmax nm
-1 -1 1 (log ε) 215 (3.6); IR max cm : 3224, 2940, 1739, 1595, 1355, 1078 cm . H NMR (500
13 MHz, CD3OD) and C NMR (125 MHz, CD3OD), see Table 4.1; HRESI-MS m/z
+ 687.3728 [M+Na] (calcd for C36H56NaO11 687.3720).
Strophanthidin 3-O-β-diginopyranosyl-(1->4)-O-β-cymaropyranosyl-(1->4)-O-β- digitoxopyranoside (4.3, madagascarensilide C)
23 Compound 4.3 was a white solid; [α]D +21 (c 0.15, MeOH); UV (MeOH) λmax nm
-1 -1 1 (log ε) 215 (3.9); IR max cm : 3459, 2939, 1740, 1374, 1072 cm . H NMR (500 MHz,
68 d-pyridine) and 13C NMR (125 MHz, d-pyridine), see Table 4.1; HRESI-MS m/z
+ 845.4315 [M+Na] (calcd for C43H66NaO15 845.4299).
Strophanthidin 3-O-β-glucopyranosyl-(l->4)-O-β-diginopyranosyl-(1->4)-O-β- cymaropyranosyl-(1->4)-O-β-digitoxopyranoside (4.4, madagascarensilide D)
23 Compound 4.4 was a white solid; [α]D +16 (c 0.51, MeOH); UV (MeOH) λmax nm
-1 -1 1 (log ε) 216 (4.1); IR max cm : 3445, 2937, 1736, 1372, 1074 cm . H NMR (500 MHz,
13 CD3OD) and C NMR (125 MHz, CD3OD), see Table 4.1; HRESI-MS m/z 1007.4844
+ [M+Na] (calcd for C49H76NaO20 1007.4828).
References
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Rakotonandrasana, S.; Rakotobe, E.; Rasamison, V. E.; Kingston, D. G. I., Four
Diphenylpropanes and a Cycloheptadibenzofuran from Bussea sakalava from the
Madagascar Dry Forest. J. Nat. Prod. ASAP.
2. Pan, E.; Harinantenaina, L.; Brodie, P. J.; Callmander, M.; Rakotonandrasana, S.;
Rakotobe, E.; Rasamison, V. E.; TenDyke, K.; Shen, Y.; Suh, E. M.; Kingston, D. G.
I., Cardenolides of Leptadenia madagascariensis from the Madagascar dry forest.
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E. M.; Kingston, D. G. I., Antiproliferative Compounds from Pongamiopsis
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study of Leptadenia heterophylla. Fitoterapia 1980, 51, 321-323.
7. Lhinhatrakool, T.; Sutthivaiyakit, S., 19-Nor- and 18,20-epoxy-cardenolides from the
leaves of Calotropis gigantea. J. Nat. Prod. 2006, 69, 1249-1251.
8. Krishna, P. V. G.; Rao, E. V.; Rao, D. V., Crystalline principles from leaves and
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11. Nikiema, J. B.; Vanhaelen-Fastre, R.; Vanhaelen, M.; Fontaine, J.; De Graef, C.;
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70 13. Cioffi, G.; Sanogo, R.; Vassallo, A.; Dal Piaz, F.; Autore, G.; Marzocco, S.; De
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Leptadenia reticulata Wight and Arn. Tetrahedron 1994, 50, 789-798.
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toxicological evaluation of alkaloids from Leptadenia pyrotechnica. Pharm. Biol.
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Nat. Rev. Drug Discovery 2008, 7, 926-935.
71 22. Nakamura, T.; Goda, Y.; Sakai, S.; Kondo, K.; Akiyama, H.; Toyoda, M.,
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Madagascar rainforest. J. Nat. Prod. 2008, 71, 325-329
72 V. Isolation and Synthesis of Antiproliferative Eupolauridine Alkaloids of Ambavia
gerrardii from the Madagascar Dry Forest
This chapter is a slightly expanded version of a prepared manuscript.1 Attributions from co-authors of the article are described as follows in the order of the names listed.
The author of this dissertation (Mr. Ende Pan) conducted isolation and structure elucidation of the titled compounds, and drafted the manuscript. Dr. Shugeng Cao was a mentor for this work, and in particular, he provided invaluable advice and hints for structure elucidation of those compounds, and he also proofread the manuscript before submission. Ms. Peggy Brodie performed the A2780 bioassay on the isolated fractions and compounds. Dr. Martin Callmander and Dr. Stephan Rakotonandrasana from
Missouri Botanical Garden did the plant collections and identification. Dr. Etienne
Rakotobe, and Dr. Vincent E. Rasamison from Madagascar carried out the initial plant extraction. Dr. Karen TenDyke, Dr. Yongchun Shen, and Dr. Edward M. Suh from Eisai
Inc. performed the H460 bioassay on the isolated compounds. Dr. David G. I. Kingston was a mentor for this work and the corresponding author for the published article. He provided critical suggestions for this work and crucial revisions to the manuscript.
5.1 Introduction
In our continuing search for biologically active natural products from tropical rainforests as part of an International Cooperative Biodiversity Group (ICBG) program, we obtained an ethanol extract from the root of a plant identified as Ambavia gerrrardii
(Baill.) Le Thomas (Annonaceae) from Madagascar. The extract exhibited good antiproliferative activity against the A2780 human ovarian cancer cell line with an IC50
73 value of 10 g/mL. On the basis of the activity and the absence of previous phytochemical study on this species, A. gerrardii was selected for fractionation to isolate its active components under the guidance of our bioassay.
5.1.1 Previous investigations of Annonaceae
The genus Ambavia (Annonaceae) consists of only two species, A. capuronii and A. gerrardii, both of which are endemic to Madagascar. Previous phytochemical studies of plant species in Annonaceae family have revealed the presence of cytotoxic acetogenins,2,3 miliusanes,4 styrylpyrones,5 polyacetylenes,6 diterpenoids,7,8 flavanones,9 and alkaloids6, 10.
Figure 5.1. Compounds isolated from the family Annonaceae 5.2 Results and Discussion
74 An EtOH extract of the roots of A. gerrardii was subjected to liquid-liquid partitioning between hexanes, CH2Cl2 and MeOH to give fractions with IC50 values of 14
µg/mL, 2.6 µg/mL and >100 µg/mL, respectively, in the A2780 assay. Fractionation of the active CH2Cl2 fraction by C18 open column and high performance liquid chromatography (HPLC) yielded the two new eupolauridine derivatives 5.1 and 5.2, as well as eupolauridine N-oxide (5.3), 8-methoxysampangin (5.4), eupolauridine (5.5) and sampangin (5.6). The structures of 5.1 and 5.2 were proposed on the basis of their UV,
IR, HRESIMS and 1H NMR spectra data, but the limited samples available combined with the absence of 13C NMR data and HMBC correlations made it necessary to confirm the structures by synthesis. The synthesis also provided material for the evaluation of the biological activities of 5.1 and 5.2. Herein we report the isolation and synthesis of 5.1 and
5.2 as well as the bioassay activities of all isolates.
Figure 5.2. Chemical structures of compounds 5.1-5.6
Eupolauridine N-oxide (5.3),11 8-methoxysampangin (5.4),12 eupolauridine (5.5)11 and sampangin (5.6)13-15 were all obtained as yellow solids. 8-Methoxysampangin was
75 isolated from a natural source for the first time. Eupolauridine has been previously isolated from the annonaceous plant Cananga odorata (Ylang ylang) collected in
Madagascar,16 and from the eupomatiaceous plant Eupomatia laurina.17
5.2.1 Structure elucidation of compound 5.1
Compound 5.1 was obtained as a yellow solid. Its UV absorptions in MeOH, with
λmax (log ε) 220 (4.15), 240 (3.98), 287 (3.82), 351 (3.28), and 369 (3.35) nm, indicated the presence of an extended aromatic chromophore, and were similar to that of eupolauridine 5.5. The IR spectroscopic data of compound 5.1, which showed absorptions at 1599, 1580, 1398, 1378, 1202, 848 and 808 cm-1, confirmed the existence of the aromatic and C-O functions. The positive ion HRESIMS of 5.1 revealed a pseudomolecular ion peak at m/z = 221.0702 [M+H]+ corresponding to a molecular
1 formula of C14H10N2O, (calcd for C14H9N2O: 221.0715). H NMR signals 5.1 in CD3OD exhibited AB, A'B́' and A''B́''X'' multiplets at low field: [δH 8.64 d (1H, J = 6.1 Hz), 8.55
(1H, d, J = 6.1 Hz), 7.78 (1H, d, J = 8.2 Hz), 7.61 (1H, d, J = 6.1 Hz), 7.51 (1H, d, J = 6.1
Hz), 7.42 (1H, d, J = 2.3 Hz), 6.86 (1H, dd, J = 8.2, 2.3 Hz)]. The above data together with the fact that 5.1 and 5.5 (C14H10N2) have the same unsaturation number (12) suggested that compound 5.1 was a hydroxylated eupolauridine. The position of the hydroxyl group was assigned at C-8 from the coupling patterns of the protons of the benzene ring of 5.1 [δH 7.78 (1H, d, J = 8.2 Hz), 6.86 (1H, dd, J = 8.2, 2.3 Hz) and 7.42
(1H, d, J = 2.3 Hz)] and comparison of the 1H-NMR spectroscopic data of 5.1 with those of 5.5 [δH 8.71 (2H, d, J = 6.0 Hz), 8.01 (2H, m), 7.67 (2H, d, J = 6.0 Hz), 7.55 (2H, m)].
Based on the above data, we proposed the structure of 8-hydroxyeupolauridine for compound 5.1.
76
5.2.2 Structure elucidation of compound 5.2
Compound 5.2 was also obtained as a yellow solid. It showed strong UV absorptions
(MeOH) at λmax (log ε) 225 (4.03), 254 (3.99), 292 (3.87), and 384 (3.36) nm. Its IR spectrum displayed aromatic absorptions at 1611, 1593, 1569, 1451, 1423, 846, 828 cm-1, a C-O stretch absorption at 1022 cm-1, and N-O stretch absorptions at 1487 and 1380 cm-
1. The characteristic alkyl C-H stretch absorptions were also observed at 2919 and 2851 cm-1. The positive ion HRESIMS of 5.2 displayed a pseudomolecular ion peak at m/z =
+ 251.0811 [M+H] , corresponding to a molecular formula of C15H11N2O2, (calcd for
C15H11N2O2: 251.0821). Its proton spectrum [AB, A'B́' and A''B́''X'' aromatic coupling pattern and one OCH3 group: δH 8.58 (1H, d, J = 6.0 Hz), 8.23 (1H, d, J = 7.1 Hz), 7.95
(1H, d, J = 8.2 Hz), 7.92 (1H, d, J = 2.4 Hz), 7.79 (1H, d, J = 7.1 Hz), 7.57 (1H, d, J = 6.0
Hz), 7.09 (1H, dd, J = 8.2, 2.4 Hz)] was very similar to that of 5.1 except for the presence of a signal for an additional methyl group at δ 3.96 (3H, s). The above spectroscopic data thus suggested compound 5.2 to be either 8-methoxyeupolauridine 1-oxide or 9- methoxyeupolauridine 1-oxide, but the available data did not permit a distinction between these two structures. It was thus necessary to synthesize 5.1 and 5.2 to confirm the structure of 5.1 and to provide additional material for bioassay, and to determine the position of the N-oxide on 5.2.
5.2.3 Synthesis of compounds 5.1 and 5.2
The synthesis of 8-hydroxyeupolauridine initially followed Wong’s method for synthesizing eupolauridine,18 using 5-methoxy-1,3-indanedione as the starting material
77 instead of the 1,3-indanedione of the published method. The key step to afford 7- methoxyonychin (5.9) involved the thermal rearrangement of an oxime O-crotyl ether, and this gave a very low yield of product (<10%) in our hands. We thus changed the synthetic strategy by modifying Bracher’s synthesis of eupolauridine (Scheme 1).19 Ethyl
3-(4-methoxyphenyl)-3-oxopropionate was deprotonated by NaH, which then underwent
Michael addition with crotonaldehyde to afford the intermediate α-(1-methyl-3- oxopropyl)-β-oxo-4-methoxyl-benzenepropanoic acetate, which reacted with hydroxylamine hydrochloride to give ethyl 2-(4-methoxy)phenyl-4-methyl-3- pyridinecarboxylate 5.7.
Scheme 5.1. Synthesis of 5.1, 5.2, and 5.11.
Although polyphosphoric acid was reported to catalyze the formation of onychin from ethyl 2-phenyl-4-methyl-3-pyridinecarboxylate in good yield,19 a very low yield of product was obtained when 5.7 was used as a substrate in the study. Compound 5.7 was
78 thus hydrolyzed to the corresponding nicotinic acid 5.8, which was then converted to its acyl chloride by treatment with thionyl chloride. A Friedel-Crafts reaction of the acyl chloride in chlorobenzene under reflux gave 7-methoxy onychin 5.9. The acidic methylene group of 5.9 reacted with dimethylformamide diethyl acetal to afford an enamine intermediate, which yielded 8-methoxy eupolauridine 5.10 through a ring closing reaction of the intermediate in the presence of ammonium acetate at 140 ºC.
Cleavage of the methyl ether of 5.10 in 48% hydrobromic acid gave 8- hydroxyeupolauridine in 1.7 % overall yield from ethyl 3-(4-methoxyphenyl)-3- oxopropionate. Comparison of the 1H NMR (Table 5.1) and HRESIMS data of 8- hydroxyeupolauridine with the data of compound 5.1 confirmed the proposed structure of the isolated natural product.
Mono-oxidation of 8-methoxyeupolauridine 5.10 by one equivalent of meta- chloroperoxybenzoic acid yielded two methoxyeupolauridine N-oxides in a 3.7:1 ratio in
2.7 % overall yield; the 1H NMR data for the major product matched the corresponding data of the natural product 5.2, and differed from that of the isomeric product 5.11.
Comparison of the 1H NMR data of 5.2 and 5.11 (Table 5.1) indicated that the proton signals of H-10 for 5.2 [δH 7.95 (d, J = 8.2 Hz)] were less deshielded than those of 5.11
[δH 8.21 (d, J = 8.4 Hz). On the other hand the signals for H-7 of 5.2 [δH 7.92 (d, J = 2.4
Hz)] were more deshielded than those of 5.11 [δH 7.61 (d, J = 2.3 Hz)].
79
Figure 5.3 Favored resonance structures of 5.2 and 5.11.
These differences enabled the structures of 5.2 and 5.11 to be assigned. The larger deshielding of H-10 of 5.11 and the larger deshielding of H-7 of 5.2 are consistent with the major resonance structures of each compound (Figure 5.2). HMBC correlations of 5.2 from both downfield shifted H-5 (δH 8.23) and H-7 (δH 7.92) to C-6 (δC 144.0) confirmed it to be the 9-methoxyeupolauridine 1-oxide.
80 Table 5.1 1H and 13C NMR spectra of compound 5.1, 5.2, 5.10 and 5.11a
5.1 5.2 5.10 5.11 position 1H (J, Hz) 13C 1H (J, Hz) 13C 1H (J, Hz) 1H (J, Hz)
2 8.55 d (6.1) 150.3 8.58 d (6.0) 149.6 8.57 d (5.7) 8.16 d (7.1)
3 7.61 d (6.1) 117.9 7.57 d (6.0) 117.8 7.28 d (6.0) 7.72 d (7.1)
3a 137.0 130.4
3b 122.7 125.0
4 7.51 d (6.1) 119.7 7.79 d (7.1) 124.4 7.35 d (5.7) 7.65 d (5.8)
5 8.64 d (6.1) 150.5 8.23 d (7.1) 145.8 8.63 d (5.7) 8.65 d (5.8)
6a 163.1 144.0
6b 143.0 136.3
7 7.42 d (2.3) 112.3 7.92 d (2.4) 113.5 7.49 d (1.4) 7.61 d (2.3)
8 164.2 164.5
9 6.86 dd (8.2, 2.3) 118.2 7.09 dd (8.2, 2.4) 117.1 6.89 dd (8.2, 1.4) 7.07 dd (8.4, 2.3)
10 7.78 d (8.2) 125.5 7.95 d (8.2) 125.4 7.81 d (8.2) 8.21 d (8.4)
10a 130.9 130.7
10b 163.6 159.6
OCH3 3.96 s 56.5 3.90 s 3.96 s a In CD3OD, δ (ppm); multiplicities; J values (Hz) in parentheses.
5.2.4 Antiproliferative activities of compounds 5.1-5.6, 5.10 and 5.11
Previous research has shown eupolauridine to have antifungal activity,20 and sampangin derivatives to have cytotoxic, antimalarial, and antifungal activities.21 8- hydroxyeupolauridine 5.1, 9-methoxyeupolauridine 1-oxide 5.2, 8-methoxyeupolauridine
5.10, and 8-methoxyeupolauridine 1-oxide 5.11, as well as eupolauridine N-oxide 5.3, 8- methoxysampangin 5.4, eupolauridine 5.5, and sampangin 5.6 were tested against the
81 A2780 human ovarian cancer cell line. Among these eight compounds, sampangin (5.6) was the most active against the A2780 cell line with an IC50 value of 0.60 M, but sampangin derivative 5.4 showed much weaker activity, with IC50 10.3 M. Among the eupolauridine analogues, compound 5.3 was the most active with an IC50 value of 3.5
M.
When tested against the H460 human lung cancer cell line, compounds 5.4 and 5.6 both exhibited strong activity with IC50 values of 0.57 M and 0.58 M, respectively.
Interestingly, only 5.3 among the eupolauridine analogues showed antiproliferative activity against this cell line, with an IC50 value of 1.77 M.
Table 5.2 IC50 (M) values of 5.1-5.6, 5.10 and 5.11 against the A2780 and H460 cancer cell lines
IC50 (M) Cancer cell line 5.1 5.2 5.3 5.4 5.5 5.6 5.10 5.11
A2480 12.7 54.3 3.5 10.3 35.7 0.60 11.1 50.3
H460 n/a n/a 1.77 0.57 n/a 0.58 n/a n/a
5.3 Experimental Section
5.3.1 General experimental procedures
UV and IR spectra were measured on a Shimadzu UV-1201 spectrophotometer and a
MIDAC M-series FTIR spectrophotometer, respectively. Melting points were obtained on a B-540 Büchi melting-point apparatus. NMR spectra were recorded in CDCl3,
CD3OD or D2O on either JEOL Eclipse 500 or Bruker 600 spectrometers. The chemical shifts are given in δ (ppm) and coupling constants (J) are reported in Hz. Mass spectra were obtained on an Agilent 6220 TOF Mass Spectrometer. HPLC was performed on a
82 Shimadzu LC-10AT instrument with a semi-preparative C18 or phenyl Varian Dynamax column (5 m, 250 x 10 mm).
5.3.2 Plant material
Roots of Ambavia gerradii (Baill.) Le Thomas (Annonaceae) were collected on July
15, 2005, in the Ambohibe dry forest near the village of Betsimiranja, Diana,
Antsiranana, Madagascar. The collection coordinates were 13° 02' 42" S, 049° 09' 11" E, and the elevation was 50 m. The plant sampled was a tree of about 16 m height with a diameter at breast height of 35 cm, and with green fruit; it occurs commonly in this area.
The tree was identified by R. Randrianaivo, and its identity was confirmed by G.E.
Schatz (Missouri Botanical Garden). Voucher specimens with voucher number RIR 1196 have been deposited in herbaria at the Parc Botanique and Zoologique de Tsimbazaza
(TAN), at the Centre National d'Application des Recherches Pharmaceutiques in
Antananarivo, Madagascar (CNARP), at the Missouri Botanical Garden in St. Louis,
Missouri (MO), and at the Muséum National d'Histoire Naturelle in Paris, France (P).
5.3.3 Extraction and Isolation
Dried roots of Ambavia gerrardii (250 g) were ground in a hammer mill, then extracted with EtOH by percolation for 24 hours at room temperature to give the crude extract MG 3311 (6.4 g), of which 3.0 g was available at Virginia Polytechnic Institute and State University (VPISU) for evaluation. The fractionation tree is shown in Scheme
5.2. The extract MG 3311 (IC50 3.6 μg/mL, 1.3 g) was suspended in aqueous MeOH
83 (MeOH-H2O, 9:1, 100 mL) and extracted with hexanes (3 x 100 mL portions). The aqueous layer was then diluted to 60% MeOH (v/v) with H2O and extracted with CH2Cl2
(3 x 150 mL portions). The hexanes extract was evaporated in vacuo to leave 138 mg with an IC50 value of 14 μg/mL. The residue from the CH2Cl2 extract (354 mg) had an
IC50 value of 2.6 μg/mL. The aqueous MeOH extract (715 mg) was inactive. The CH2Cl2 extract was selected for fractionation, and six fractions of 124, 44, 81, 26, and 38 mg were collected from a C18 open column eluted with MeOH/H2O (gradient from 60% to
100%). The first four fractions had IC50 values of 3.7, 1.9, 3, and 16 μg/mL, respectively, and the last fraction was inactive. Fractions I and II were selected for further work.
Separation of fraction I by C18 preparative HPLC (60% MeOH-H2O) yielded eight subfractions (IC50 19 μg/mL, inactive, inactive, 3.2 μg/mL, inactive, 2 μg/mL, 4.9 μg/mL and inactive), and the most active subfractions I-4 (IC50 3.2 μg/mL), and I-6 (IC50 2
μg/mL) were selected for further separation by phenyl HPLC (60% MeOH-H2O).
Compounds 5.1 (0.4 mg, tR 28.1min) and 5.3 (2.0 mg tR 31.4 min) were isolated from subfraction I-4, and compounds 5.2 (0.5 mg, tR 29.8 min), and 5.4 (1.0 mg, tR 36.0 min) were isolated from subfraciton I-6. Fraction II was applied to a C18 open column. The most active subfraction (IC50 0.14 g/mL) was subjected to phenyl HPLC (70% MeOH-
H2O) to give compounds 5.5 (1.9 mg, tR 25.3 min) and 5.6 (0.6 mg, tR 31.4 min).
84
Scheme 5.2 Separation of ethanol extract of Ambavia gerradii
8-Hydroxyeupolauridine (5.1)
Yellow solid; UV (MeOH) λmax nm (log ε) 220 (4.15), 240 (3.98), 287 (3.82), 351
-1 (3.28), 369 (3.35); IR max cm : 1638, 1599, 1580, 1398, 1378, 1289, 1243, 1202, 1091,
-1 1 1060, 1016, 994, 842 and 808 cm . H NMR (500 MHz, CD3OD), see Table 5.1; HRESI-
+ MS m/z 221.0713 [M+H] (calcd for C14H10N2O, 221.0715).
9-Methoxyeupolauridine 1-oxide (5.2)
Yellow solid; UV (CD3OD) λmax nm (log ε) 225 (4.03), 254 (3.99), 292 (3.87), 384
-1 (3.36); IR max cm : 2919, 2851, 1611, 1593, 1487, 1451, 1423, 1380, 1256, 1233, 1022,
85 -1 1 13 973, 846 and 828 cm . H NMR (500 MHz, CD3OD) and C NMR (125 MHz, CD3OD),
+ see Table 5.1; HRESI-MS m/z 251.0820 [M+H] (calcd for C15H11N2O2, 251.0821).
Eupolauridine N-oxide (5.3)
+ 1 Yellow solid; LC-MS m/z 221.1 [M+H] (calcd for C14H9N2O, 221.1). H NMR (500
MHz, CDCl3), see appendix.
8-Methoxysampangin (5.4)
+ 1 Yellow solid; LC-MS m/z 263.1 [M+H] (calcd for C16H11N2O2, 263.1). H NMR
(500 MHz, CDCl3), see appendix.
Eupolauridine (5.5)
+ 1 Yellow solid; LC-MS m/z 205.1 [M+H] (calcd for C14H9N2, 205.1). H NMR (500
MHz, CDCl3), see appendix.
Sampangin (5.6)
+ 1 Yellow solid; LC-MS m/z 233.0 [M+H] (calcd for C15H9N2O, 233.1). H NMR (500
MHz, CDCl3), see appendix.
5.3.4 Synthesis of 8-hydroxy eupolauridine and 9-methoxyl eupolauridine 1-oxide
Ethyl 2-(4-methoxy)phenyl-4-methyl-3-pyridinecarboxylate (5.7)
To a solution of 3.84 g (17 mmol) of ethyl 3-(4-methoxyphenyl)-3-oxopropionate in
14 mL dioxane in a 100 mL flask was added 48 mg (2 mmol) NaH, and then 1.68 g (24
86 mmol) crotonaldehyde dropwise in 6 mL dioxane. After the reaction mixture was stirred for another 30 min at room temperature, 4.9 g of H2NOH.HCl (70 mmol) and 20 mL glacial AcOH were added. The reaction mixture was stirred at 100-110 ºC for 90 minutes and then poured onto 100 g of ice, made basic with K2CO3 and extracted with ether (3 x
100 mL). The combined organic phase was extracted with 2N HCl (3 x 100 mL). The combined acidic aqueous phase was neutralized with K2CO3 and extracted with ether (3 x
100 mL), and the ether was dried over K2CO3 and evaporated under reduced pressure.
The oily crude product was purified by flash silica column chromatography
(hexane:EtOAc, 4:1) to afford ethyl 2-(4-methoxy)phenyl-4-methyl-3-
1 pyridinecarboxylate 5.7 (1.47 g, 32%). Yellow oil. H NMR (500 MHz, CDCl3) δH 8.53
(1H, d, J = 5.1 Hz), 7.54 (2H, d, J = 8.8 Hz), 7.08 (1H, d, J = 5.1 Hz), 6.94 (2H, d, J = 8.8
Hz), 4.17 (2H, q, J = 7.2 Hz), 3.82 (3H, s), 2.39 (3H, s), 1.08 (3H, t, J = 7.2 Hz). 13C
NMR (125 MHz, CDCl3) δC 168.8, 160.0, 156.0, 149.4, 145.4, 132.4, 129.6, 128.9,
123.1, 113.7, 61.3, 55.2, 19.3, 13.7. HRESI-MS m/z 272.1268 [M+H]+ (calcd for
C16H18NO3, 272.1287).
2-(4-Methoxyphenyl)-4-methyl-3-pyridinecarboxylic acid (5.8)
Compound 5.7 (670 mg, 2.5 mmol) was refluxed overnight in aqueous NaOH (40%,
10 mL). The solution was extracted with CHCl3 to remove any unreacted starting material. The pH value of the mixture was then adjusted to about 6, the solvent was removed under reduced pressure, and the residue was extracted with hot MeOH (3 x 10 mL). The MeOH extracts were combined and concentrated to a residue which was purified on a silica column eluted with MeOH:CH2Cl2 (3:1) to give 2-(4-
87 methoxy)phenyl-4-methyl-3-pyridinecarboxylic acid 5.8 (403 mg, 66%). Off-white solid,
1 Mp 198-200ºC. H NMR (500 MHz, D2O) δH 8.43 (1H, d, J = 6.2 Hz), 7.78 (1H, d, J =
6.2 Hz), 7.62 (2H, d, J = 9.0 Hz,), 7.13 (2H, d, J = 9.0 Hz), 3.89 (3H, s), 2.59 (3H, s). 13C
NMR (125 MHz, D2O) δC 171.6, 161.8, 155.4, 147.6, 139.1, 130.3, 126.7, 123.6, 115.0,
+ 55.7, 49.0, 20.2. HRESI-MS m/z 244.0958 [M+H] (calcd for C14H14NO3, 244.0974).
7-Methoxyonychin (5.9)
A solution of 5.8 (93 mg, 0.38 mmol) in 2 mL SOCl2 was refluxed 24 h under N2.
After the solvent was removed under vacuum, 51 mg (0.38 mmol) AlCl3 and 2 mL chlorobenzene were added to the flask and the mixture refluxed overnight under N2. The reaction was cooled and quenched by pouring into 10 mL saturated NaHCO3 solution.
The resulting mixture was extracted with CH2Cl2 (3 x 10 mL), dried over K2CO3, and the
CH2Cl2 phase was evaporated. The residue was purified by silica gel PTLC
(hexanes:EtOAc, 6:4) to afford 7-methoxyonychin (5.9) (21 mg, 25%). Yellow solid, Mp
1 134-136ºC. H NMR (500 MHz, CDCl3) δH 8.30 (1H, d, J = 5.2 Hz), 7.79 (1H, d, J = 8.2
Hz), 7.18 (1H, d, J = 2.3 Hz), 7.04 (1H, dd, J = 8.2, 2.3 Hz), 6.90 (1H, d, J = 5.2 Hz),
13 3.87 (3H, s), 2.59 (3H, s). C NMR (125 MHz, CDCl3) δC 192.4, 164.8, 162.5, 151.3,
148.2, 136.9, 134.5, 126.1, 125.0, 122.7, 120.4, 109.0, 55.8, 17.3. HRESI-MS m/z
+ 226.0865 [M+H] (calcd for C14H12NO2, 226.0868).
8-Methoxyeupolauridine (5.10)
A solution of 33 mg (0.15 mmol) of 5.9, plus N,N-dimethylformamide diethyl acetal in 1 mL DMF was stirred 2 h at 120 ºC under N2. NH4OAc (600 mg) was added to the
88 flask and the reaction mixture was stirred for another 30 min at 140 ºC, quenched with 5 mL water, and the solvent was extracted with EtOAc (3 x 5 mL). The organic phase was combined, dried over K2CO3 and concentrated to a residue which was separated on a silica TLC plate (hexanes:EtOAc, 6:4) to give 8-methoxy eupolauridine 5.10 (25.4 mg,
1 74%). Yellow solid, Mp 138-140ºC. H NMR (500 MHz, CDCl3) δH 8.63 (1H, d, J = 5.7
Hz), 8.57 (1H, d, J = 5.7 Hz), 7.81 (1H, d, J = 8.2 Hz), 7.49 (1H, d, J = 1.4 Hz), 7.35 (1H, d, J = 5.7 Hz), 7.28 (1H, d, J = 5.7 Hz), 6.89 (1H, dd, J = 8.2, 1.4 Hz), 3.89 (3H, s). 13C
NMR (125 MHz, CDCl3) δC 162.8, 162.5, 162.2, 149.7, 149.6, 142.0, 134.9, 131.9,
123.7, 121.3, 117.8, 116.3, 115.5, 109.0, 55.7. HRESI-MS m/z 235.0875 [M+H]+ (calcd for C15H11N2O, 235.0871).
8-Hydroxyeupolauridine (5.1)
A solution of 5.10 (6 mg, 0.026 mmol) in 1 mL of 48% HBr was refluxed for 24 h.
The mixture was then cooled and evaporated in vacuo to give a residue which was purified by PTLC on silica gel (hexanes:EtOAc, 6:4). 8-Hydroxyeupolauridine 5.1 (2.5 mg, 43 %) was collected as a yellow solid. Mp 278-280ºC. Its 1H NMR and HRESI-MS spectrum were identical to those of the isolated material. 13C NMR, see Table 5.1.
Preparation of 8-methoxyeupolauridine 1-oxide (5.11) and 9-methoxyeupolauridine 1- oxide (5.2)
A solution of 8-methoxyeupolauridine (5.10, 10 mg, 0.045 mmol) and meta- chloroperoxybenzoic acid, (7.4 mg, 0.043 mmol) in 1 mL CH2Cl2 was stirred at room temperature for 24 h. After the solvent was removed, the residue was separated by C18
89 HPLC (60% MeOH:H2O) to afford yellow solid 9-methoxyeupolauridine 1-oxide 5.2 (5.9 mg, 55%) (Mp 198-200ºC) and 8-methoxyeupolauridine 1-oxide 5.11 (1.6 mg, 15%). The
NMR and mass spectroscopic data for 5.2 were identical to those of the natural product.
8-Methoxyeupolauridine 1-oxide (5.11)
1 Yellow solid, Mp 218-220ºC. H NMR (600 MHz, CD3OD): δH 8.65 (1H, d, J = 5.8
Hz), 8.21 (1H, d, J = 8.5 Hz), 8.16 (1H, d, J = 7.1 Hz), 7.72 (1H, d, J = 7.1 Hz), 7.65 (1H, d, J = 5.8 Hz), 7.61 (1H, d, J = 2.3 Hz), 7.07 (1H, dd, J = 8.4, 2.4 Hz), 3.96 (3H, s). 13C
NMR (150 MHz, CD3OD) of 5.11: δC 164.6, 159.3, 149.8, 145.6, 144.5, 141.1, 130.7,
128.4, 126.3, 125.3, 123.1, 119.3, 116.5, 111.2, 56.5. HRESI-MS m/z 251.0807 [M+H]+
(calcd for C15H11N2O2, 251.0821).
5.3.5 Antiproliferative Bioassays
The A2780 ovarian cancer cell line assay was performed at Virginia Polytechnic
Institute and State University as previously reported,22 except that the samples were added in 1 L 100% DMSO per well instead of 20 L of 1:1 DMSO:H2O. The A2780 cell line is a drug-sensitive human ovarian cancer cell line.23 Assays against the NCI-
H460 lung cancer cell line were carried out at Eisai, Inc., as previously described.1
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94 VI. Five new antimalarial pseudoguaianolides of Polycline proteiformis from the
Madagascar dry forest
6.1 Introduction
In our continuing search for biologically active natural products from tropical rainforests, we obtained an ethanol extract from the aerial part of a plant identified as
Polycline proteiformis Humbert (Asteraceae) from Toliara, Madagascar. The extract exhibited good antimalarial activities against the chloroquine-sensitive HB3 strain and the chloroquine-resistant Dd2 strain of the malaria with IC50 value of 1.9 g/mL (HB3) and 1.6 g/mL (Dd2). On the basis of the activities and the absence of previous phytochemical study on this species, P. proteiformis was selected for fractionation to isolate its active components under the guidance of our bioassay.
6.1.1 Previous investigations of Asteraceae
There are only four species in the genus Polycline (Asteraceae): P. gracilis, P. proteiformis, P. psyllioides, and P. stuhlmannii. Previous phytochemical studies of plants belonging to the family Asteraceae have revealed the presence of antimalarial sesquiterpene lactones1-5 and flavonoids.6-7 Among all the compounds isolated from natural resources (from alkaloids, terpenes, flavonoids, limonoids, chalcones, peptides, xanthones, quinones and coumarins),8 the sesquiterpenoid artemisinin from a traditional
Chinese medicinal plant, Artemesia annua (Asteraceae)1 is still one of the best antimalarial agent available. Its derivatives have been widely used in artemisinin-based combination therapies (ACTs).9-10 The structures of artemisin and a representative set of compounds isolated from Asteraceae are shown in Figure 6.1.
95
Figure 6.1 Artemisinin and compounds isolated from the family Asteraceae
6.2 Results and Discussion
An EtOH extract of aerial parts of P. proteiformis was subjected to liquid-liquid partitioning to give hexanes, dichloromethane (CH2Cl2) and methanol fractions with IC50 values of 4 µg/mL, 1.5 µg/mL, and >10 µg/mL, respectively against the HB3 malaria parasite, and 2 µg/mL, 1.0 µg/mL and 8 µg/mL respectively against the Dd2 malaria parasite. Fractionation by C18 open column and High Performance Liquid
Chromatography (HPLC) of the active CH2Cl2 fraction yielded five new sesquiterpene lactones (6.1-6.5), as well as a flavonoid 6.6. Herein we report the structural elucidation, the antimalarial properties and the cytotoxicities of the isolates.
96
Figure 6.2 Chemical structures of polyclinolides A-E (6.1-6.5) and centaureidin (6.6)
6.2.1 Structure elucidation of polyclinolide A (6.1)
Polyclinolide A (6.1) was obtained as an off-white needle crystal. Its positive ion
HRESIMS revealed a pseudomolecular ion peak at m/z 437.2176 [M+H]+, corresponding to the molecular formula of C23H32O8. The IR spectrum showed strong absorption in the range 1740-1710 cm-1 which was consistent with the presence of ester, and lactone
1 groups. Its H NMR spectrum in CDCl3 showed four singlet methyl groups at δH 2.10,
2.09, 2.08 and 0.98, three doublet methyl groups at δH 1.16, 1.16 and 1.05, one exocyclic methylene (δH 6.27 and 5.65, each doublet, J = 3.2 Hz, H-13a and H-13b), two sets of methylenes at δH 2.64 (m, H-3a) and δH 1.32 (dd, J = 16.3, 2.7 Hz, H-3b), and at δH 2.33
(dt, J = 12.8, 3.1 Hz, H-9a) and δH 1.55 (m, H-9b), eight methine resonances [δH 1.88, m
(H-10); δH 2.67, m (H-1); δH 2.52, septet, J = 7.0 Hz (H-2'); δH 3.48, m (H-7); δH 4.11, ddd, J = 12.2, 9.0, 3.5 Hz (H-8); δH 4.95, d, J = 4.9 Hz (H-4); δH 5.03, ddd, J = 8.7, 8.7,
97 2.7 Hz, (H-2) and δH 5.06, d, J = 11 Hz (H-6), four of which were oxygenated (Table
13 6.1). The C NMR spectrum displayed signals arising from two acetoxy groups (δC
169.8 and 20.0) and (δC 170.2 and 21.4), one isobutyroxy group (δC 176.4, 33.9, 18.8 and
18.7) together with 15 signals [two quaternary carbons at δC 51.5 (C-5), 136.2 (C-11), a lactone carbonyl at δC 169.2 (C-12), one quaternary and one secondary methyls at δC 22.4
C-15) and 20.0 C-14), two methylenes at δC 37.9 (C-3) and 43.1 (C-9), four oxygen- bearing methines at δC 80.9 (C-8), 79.6 (C-4), 78.1 (C-6) and 75.5 (C-2), three methines at δC 51.3 (C-1), 46.3 (C-7) and 27.2 (C-10), an exocyclic methylene at δC 124.1 (C-13), as indicated by the HMQC spectrum (Table 6.1)] assignable to a sesquiterpene lactone.
Inspection of the 1H NMR spectral data revealed that polyclinolide A is very similar to pseudoguaianolides 6-angeloyloxypuchellin11 and 2-deacetyl-2-isobutyryl- chamissonolide12.
The complete 1H and 13C NMR assignments and connectivities were established from a combination of HMQC, COSY and HMBC data analyses. The COSY spectrum showed correlations that indicated the presence of spin systems: H-4, H-3, H-2, H-1, H-10, H-9,
H-8, H-7, H-6, and H-14, and H-2', H-3' and H-4' of the isobutyroxy moiety. In HMBC spectrum, the correlations from H3-15 to C-4, C-5, C-1, and C-6 as well as the correlations from H-4 to C-1 and to C-5 corroborated the presence of a five member ring fused at C-1 and C-5, with a seven member ring. The HMBC correlations from H2-9 to
C-1, C-8, C-7 and C-14, and from H-6 to C-5, C-7 and C-8, and from H2-13 to C-7 and the lactone carbonyl at C-12 suggested the presence of a γ-lactone ring, fused at C-7, C-8.
These data indicated that 6.1 was a pseudoguaianolide analogue.11-12 The HMBC correlations from two methyls (H3-3' and H3-4'), one septet methine (H-2'), and H-2 to C-
98 1', H-4 and H3-2'' to C-1'', H-6 and H3-2''' to C-1''' suggested the presence of an isobutyrate group at C-2, an acetate group at C-4, and another acetate group at C-6. To determine the relative stereochemistry of 6.1, NOESY (Nuclear Overhauser Effect
Spectroscopy) experiment was carried out. In the NOESY spectrum, the correlations from H-1 to H-7, H-9b, and H3-14, from H3-15 to H-2, H-4, H-6, H-8 and H-10, from H-
8 to H-6, H-9a and H-10 suggested the α orientations of H-1, H-7 and H3-14, and the β- orientations of H-2, H-4, H-6, H-8, H-10 and H3-15.
Figure 6.3. a) Key COSY(bold) and HMBC (arrows) correlations of 6.1 b) Key NOESY correlations for 6.1.
In order to determine the absolute stereochemistry, compound 6.1 was crystallized in methanol to afford a good quality of a single crystal nd its structure was confirmed by single crystal X-ray diffraction. An anisotropic displacement ellipsoid drawing is shown in Figure 6.4. The structure of 6.1 was thus established to be
(1S,2S,4R,5S,6S,7R,8S,10R)-2-isobutyryloxy-4-acetoxy-6-acetoxyguai-11(13)-en-8,12- olide (6.1, polyclinolide A)
99
Figure 6.4 Anisotropic displacement ellipsoid drawing of 6.1
6.2.2 Structure elucidation of polyclinolide B (6.2)
Polyclinolide B (6.2) was obtained as an off-white solid. Its positive ion HRESIMS revealed a pseudomolecular ion peak at m/z 417.1889 [M+Na]+, corresponding to the
-1 molecular formula of C21H30O7. The IR spectrum showed hydroxyl function (3474 cm ), and strong absorption in the range 1740-1710 cm-1 which was consistent with the
1 presence of ester, and lactone groups. Its H NMR spectrum in CDCl3 showed signals for two singlet methyl groups at δH 2.10 and 0.98, three doublet methyl groups at δH 1.14,
1.14 and 1.02, three multiplet methylenes (δH 2.66, 1.53; 2.42, 1.41; 5.53, 6.40), two of which were olefinic protons, and eight methines (δH 1.98, 2.24, 2.50, 3.04, 4.41, 4.64,
4.90 and 5.02,), four of which were oxygenated (Table 6.1). Inspection of the 1H and 13C
NMR data of 6.2 in CDCl3 showed a close similarity with the data of the previously isolated 2,4-diacetoxy-6-hydroxyguai-11(13)-en-8,12-olide 6.7,13 except for the presence of an isobutyrate group and an acetate group instead of two acetate groups at C-2 and C-4, respectively. The HMBC correlations from H-2 to C-1', one septet methine H-2', two methyls H3-3' and H3-4' to C-1', and from H-4 to C-1'' suggested the isobutyrate group
100 was located at C-2 and the acetate group at C-4. In the NOESY spectrum, the correlations from H-1 to H-6, H-7, H-9b, and H3-14, from H-6 to H-7 and H-1, and from H3-15 to H-2,
H-4, H-8 and H-10, suggested the α orientations of H-1, H-6, H-7 and H3-14, and the β- orientations of H-2, H-4, H-8, H-10 and H3-15. The absolute configuration of 6.2 was deduced by the comparison of its CD spectral data with those of 6.1. The characteristic
UV absorption of α,β-unsaturated lactone chromophore was shown at around 230 nm.14
The negative Cotton effect [] = 1.56 x 103 of 6.2 at 230 nm, which was very similar to
3 that of 6.1 ([]230 nm = 3.57 x 10 ), assigned the S configuration of C-7 according to the back octant rule.15 Therefore, polyclinolide B (6.2) was determined to be
(1S,2S,4R,5S,6R,7S,8S,10R)-2-isobutyryloxy-4-acetoxy-6-hydroxyguai-11(13)-en-8,12- olide (6.2, polyclinolide B)
Figure 6.5 Chemical structures of 6.4 and 6.7.
6.2.3 Structure elucidation of polyclinolide C (6.3)
Polyclinolide C (6.3) was obtained as an off-white solid. Its positive ion HRESIMS revealed a pseudomolecular ion peak at m/z 483.2003 [M+Na]+, corresponding to the molecular formula of C25H32O8 for 6.3. The IR spectrum showed hydroxyl function (3444 cm-1), and strong absorption in the range 1740-1710 cm-1 which was consistent with the
1 presence of ester, ketone and lactone groups. Its H NMR spectrum in CDCl3 showed
101 signals for three singlet methyl groups (δH 1.27, 1.26 and 1.07), one doublet methyl group
(δH 1.41 d, J = 7.2 Hz), two olefinic methyl groups (δH 1.78 m; 1.94 dq, J = 7.3, 1.5 Hz), one singlet methylene (δH 2.49), and three methines (δH 3.55 m, 3.19 m and 2.43 m).
Three oxygenated methines were also observed at δH 5.48 s, 5.34 (d, J = 3.7 Hz), δH 4.66 s. In the downfield region of the spectrum, there were five olefinic methines at δH 7.60 dd
(J = 6.0, 1.6 Hz), 6.80 s, 6.12 s, 6.12 m, 6.10 m. The 13C NMR spectrum displayed a set of signals ascribable to an (Z)-2-methyl-2-butenoyl (angeloyl) group (δC 166.1, 127.0,
13 139.7, 15.7, 20.4) and a 3-hydroxy-3-methylbutanoyl group (δC 171.6, 46.4, 69.2, 29.3,
16 29.2) together with 15 signals of a sesquiterpene lactone [two quaternary carbons at δC
55.3 and 130.8, a lactone carbonyl at δC 162.4 and a conjugated ketone at δC 207.7 and one quaternary and one secondary methyl (δC 18.8 and 19.2, respectively), two olefinic methines (δC 161.0 and 130.3, three oxygen-bearing methines (δC 88.5, 76.0 and 64.7), three methines (δC 50.5, 45.5 and 50.5), and an exocyclic methylene (δC 134.4), as indicated by the HMQC spectrum (Table 6.1)]. The complete 1H and 13C NMR assignments and connectivities were established from a combination of COSY, HMQC, and HMBC data. The COSY spectrum showed correlations that indicated the presence of the two spin systems: H-3, H-2, H-1, H-10, H-9, H-8, H-7, H-6, and H-14, and H-3' and
H-4' of the angeloyl moiety. In the HMBC spectrum, the correlations from H3-15 to C-4,
C-5, C-1, and C-6 as well as the correlations from H-3 to C-4 and C-5 corroborated the presence of an α, β-unsaturated five member ketone ring fused with a seven member ring.
The HMBC correlations from H-9 to C-12 at δC 162.4 and H-7 to C-11 at δC 130.8, C-12 at δC 162.4 and C-13 at δC 134.4 suggested the presence of a δ-lactone ring, fused at C-7,
C-8 and C-9. These data indicated that 6.3 was a pseudoguaianolide analogue.17
102 Inspection of the 13C NMR data of 6.3 indicated a close similarity to those of pseudoguaianolides previously isolated from Hymenoxys ivesiana.17 Spectrally, 6.3 differed from compound 6.8 only in the nature of the ester substituent at C-8. The locations of the (Z)-2-methyl-2-butenoate (angelate) group at C-6 and the 3-hydroxy-3- methyl butanoate group at C-8 were substantiated by the observation of HMBC cross peaks from H-6 to C-1', H-3' to C-1', H-4' to C-3' and C-2', and H-5' to C-1', C-2' and C-3' and from H-8 to C-1'', H2-2'' to C-1'', C-3'', C-4'' and C-5'', H3-4'' to C-2'', C-3'', and C-5'', and H3-5'' to C-2'', C-3'', and C-4''.
Figure 6.6 a) Key COSY (bold) and HMBC (arrows) correlations for 6.3. b) Key NOESY correlations for 6.3. c) Chemical structure of compound 6.8
To determine the relative stereochemistry of 6.3, a NOESY experiment was carried out. The NOESY correlations from H3-15 to H-10 and H-6, and H3-14 to H-1, H-9 and
H-8, as well as the cross peaks arising from H-7 to H-8 suggested α orientations of H-1,
H-7, H-8, H-9 and H3-14 and β orientations of H-6, H-10 and H3-15. Therefore, polyclinolide C (6.3) was determined to be 1α,7α,10βH-4-oxo-6α-angeloyloxy-8β-(3- hydroxy-3-methyl-butanoyloxy)pseudoguaia-2(3),11(13)-dien-9β,12-olide, as depicted in
Figure 6.6.
103 6.2.4 Structure elucidation of polyclinolide D (6.4)
Polyclinolide D (6.4) was obtained as an off-white solid. Its positive ion HRESIMS revealed a pseudomolecular ion peak at m/z 497.2143 [M+Na]+, corresponding to the
-1 molecular formula of C26H34O8. The IR spectrum showed hydroxyl function (3445 cm ), and strong absorption in the range 1740-1710 cm-1 which was consistent with the
1 presence of ester, ketone and lactone groups. The H NMR data of 6.4 in CDCl3 showed a close similarity of those of 6.3, except for the presence of a set of signals at δH 2.51 (1H, d J = 15.5 Hz), 2.42 (1H, d J = 15.5 Hz), 1.52 (2H, qd J = 7.5, 2.6 Hz), 0.90 (3H, t J = 7.5
Hz) and 1.19 (3H, s) instead of those for the 3-hydroxy-3-methylbutanoate group, which indicated the presence of a different side chain at C-8 in 6.4. The 13C NMR spectrum
18 displayed signals ascribable to a 3-hydroxy-3-methylpentanoate group (δC 171.7, 44.5,
71.4, 34.7, 8.2 and 26.2). The HMBC correlations from H-8 to C-1''; H-2'' to C-1'' and C-
3''; H-4'' to C-2'', C-3'', C-5'' and C-6'', H-5'' to C-3'', and H-6'' to C-2'' and C-3'' as well as the COSY cross peak from H-5'' and H-6'' confirmed the presence of a 3-hydroxy-3- methylpentanoate group and its location to be at C-8. The NOESY spectrum of 6.4 showed similar correlations as observed for 6.3. The correlations from H3-15 to H-10 and
H-6, and H3-14 to H-1, H-9 and H-8, as well as the correlation from H-7 to H-8 were observed. Thus the structure of 6.4 was concluded to be 1α,7α,10βH-4-oxo-6α- angeloyloxy-8β-(3-hydroxy-3-methyl-pentanoyloxy)pseudoguaia-2(3),11(13)-dien-
9β,12-olide.
104
Figure 6.7 COSY (bold) and HMBC (arrows) correlations for 3-hydroxy-3-methylpentanoate group at C-8 of 6.4.
6.2.5 Structure elucidation of polyclinolide E (6.5)
Polyclinolide E (6.5) was obtained as an off-white solid. Its positive ion HRESIMS revealed a pseudomolecular ion peak at m/z 489.2496 [M+H]+, corresponding to the
-1 molecular formula of C27H36O8. The IR spectrum showed hydroxyl function (3441 cm ), and strong absorption in the range 1740-1710 cm-1 which was consistent with the presence of ester, ketone and lactone groups. The similarity of the 1H and 13C NMR spectroscopic data of 6.5 as those of 6.4 (Table 6.1) suggested that 6.5 was a close related pseudoguaianolide analogue. The presence of an (E)-3-methyl-2-pentenoate group at C-6 was indicated by the observation of a set of signals at δH 5.53 (1H, s), 2.16 (2H, q J = 7.3
Hz), 2.15 (3H, s) and 1.05 (3H, t J = 7.3 Hz) in the 1H NMR spectrum, and the
13 corresponding C NMR data (δC 165.5, 113.3, 164.4, 33.9, 11.8 and 19.0) supported this conclusion.19 The HMBC correlations from H-6 to C-1', H-2'' to C-1'', C-3'', H-4'' to C-2',
C-3', C-5' and C-6', H-5' to C-3' and C-4',and H-6' to C-2', C-3' and C-4' as well as the
COSY cross peak from H-4' and H-5' confirmed an (E)-3-methyl-2-pentenoate group at
C-6. Moreover, the NOESY correlation observed in 6.5 were similar to those observed in
6.3 and 6.4. Clear correlations from H3-15 to H-10 and H-6, from H3-14 to H-1, H-9 and
105 H-8, from H-7 to H-8 of 5 as well as from H-2' to H-4' were observed. Thus the structure of 6.5 was concluded to be 1α,7α,10βH-4-oxo-6α-(E)-3-methyl-2-pentenoylxy-8β-(3- hydroxy-3-methyl-pentanoyloxy)pseudoguaia-2(3),11(13)-dien-9β,12-olide.
6.2.6 Structure elucidation of compound (6.6)
Compound 6.6 was determined to be the previously isolated flavonoid centaureidin by comparison of its MS and 13C NMR data with literature data.20
6.2.7 Bioactivities of polyclinilide A-E (6.1-6.5) and centaureidin (6.6)
Polyclinolide C (6.3) and D (6.4) were tested for their antimalarial activities against
HB3 and Dd2 malaria parasites. Polyclinolide C (6.3), showed strong activities against both malaria parasites with IC50 values of 0.7 M in each case. Polyclinolide D (6.4) had similar activities (IC50 0.8 M) against both parasites.
The cytotoxicities of compounds 6.1-6.6 were also evaluated against the A2780 human ovarian cancer cell line. Polyclinolide A-E had IC50 values of 2.2, 6.0, 0.9, 0.8, and 4.0 μM respectively against A2780 cell line. Centaureidin 6.6 was also cytotoxic with an IC50 value of 3.9 M.
Although polyclinolide C and D showed strong antimalarial activitites against both
HB3 and Dd2 strains, they will not be good lead compounds for antimalarial drug development due to their cytotoxicities.
106 Table 6.1 1H and 13C NMR chemical shifts of polyclinolides A (6.1), B (6.2), C (6.3), D (6.4) and E (6.5) a 6.1 6.2 6.3 6.4 6.5 position 1H (J, Hz) 13C 1H (J, Hz) 13C 1H (J, Hz) 13C 1H (J, Hz) 13C 1H (J, Hz) 13C
1 2.67 m 51.3 2.24 dd (10.8, 6.8) 52.8 3.19 m 50.5 3.19 m 50.5 3.23 m 50.2
2 5.03 ddd (8.7, 8.7, 2.7) 75.5 5.02 ddd (9.1, 6.9, 2.1) 77.2 7.60 bd (6.0) 161.0 7.60 bd (6.1) 161.0 7.60 bd (6.0) 161.1
2.64 m, 1.32 dd (16.3, 2.66 ddd (16.4, 9.0, 4.8), 3 37.9 38.1 6.11 m 130.3 6.11 m 130.3 6.10 m 130.3 2.7) 1.53 dd (16.5, 2.1)
4 4.95 d (4.9) 79.6 4.90 d (4.8) 82.4 207.7 207.7 207.8
5 51.5 51.3 55.3 55.3 55.2
6 5.06 (d, 11.0) 78.1 4.41 bs 65.0 5.34 d (3.7) 76.0 5.34 d (3.7) 76.0 5.31 d (3.7) 75.3
7 3.48 m 46.3 3.04 dq (9.1, 3.4) 52.0 3.55 m 45.5 3.56 m 45.5 3.51 m 45.7
8 4.11 ddd (12.2, 9.0, 3.5) 80.9 4.64 ddd (12.1, 9.1, 3.2) 75.7 5.48 s 64.7 5.48 s 64.7 5.49 s 65.0
2.33 dt (12.8, 3.1), 2.41 m, 9 43.1 44.0 4.66 s 88.5 4.66 s 88.5 4.66 s 88.6 1.55 m 1.41 m
10 1.88 m 27.2 1.98 m 29.0 2.43 m 35.4 2.43 m 35.4 2.43 m 35.4
11 136.2 136.8 130.8 130.8 131.2
12 169.2 169.3 162.4 162.4 162.5
6.27 d (3.2), 6.40 (d, 3.6), 13 124.1 121.0 6.80s, 6.11s 134.4 6.80s, 6.11s 134.3 6.78s, 6.10s 134.1 5.65 d( 3.2) 5.53(d, 3.6)
14 1.05 d (6.8) 20.0 1.02 d (6.6) 20.9 1.41 d (7.2) 19.2 1.41 d (7.2) 19.2 1.41 (7.2) 19.1
15 1.09 s 22.4 0.98 s 17.7 1.07 s 18.8 1.07 s 18.8 1.05 s 18.9
1' 176.4 176.4 166.1 166.1 165.5
2’ 2.52 septet (7.0) 33.9 2.50 septet (7.0) 34.0 127.0 127.0 5.53 113.3
3’ 1.16 d (7.0) 18.8 1.14 d (7.0) 18.8 6.09 m 139.7 6.09 m 139.6 164.4
4’ 1.16 d (7.0) 18.7 1.13 d (7.0) 18.7 1.94 dq (7.3, 1.5) 15.7 1.93 dq (7.2, 1.5) 15.7 2.16 q (7.3) 33.9
107 5’ 1.78 m 20.4 1.78 m 20.4 1.05 t (7.3) 11.8
6’ 2.15 s 19.0
1'' 169.8 170.5 171.6 171.7 171.8
2.51 d (15.5) 2.51 d (15.2) 2'' 2.08 s 20.0 2.10 s 21.3 2.49 s 46.4 44.5 44.6 2.42 d (15.5) 2.43d (15.2)
3'' 69.2 71.4 71.5
4'' 1.27 s 29.3 1.52 qd (7.5, 2.5) 34.7 1.52 m 34.7
5'' 1.26 s 29.2 0.90 t (7.5) 8.2 0.90 t (7.5) 8.3
6'' 1.19 s 26.2 1.19 s 26.2
1''' 2.09 s 170.2
2''' 21.4 a 1 13 In CDCl3, δ (ppm) 500 MHz for H and 125 MHz for C; multiplicities; J values (Hz) in parentheses.
108 6.3 Experimental Section
6.3.1 General experimental procedures
Optical rotations were recorded on a JASCO P-2000 polarimeter. UV and IR spectra were measured on a Shimadzu UV-1201 spectrophotometer and a MIDAC M-series
FTIR spectrophotometer, respectively. CD analysis was performed on a JASCO J-810 spectropolarimeter with a 1.0 cm cell in methanol. NMR spectra were recorded in CDCl3 on either JEOL Eclipse 500 or Bruker 600 spectrometers. The chemical shifts are given in δ (ppm) and coupling constants (J) are reported in Hz. Mass spectra were obtained on an Agilent 6220 TOF Mass Spectrometer. HPLC was performed on a Shimadzu LC-
10AT instrument with a semi-preparative C18 Varian Dynamax column (5 m, 250 x 10 mm).
6.3.2 Extraction and Isolation
Dried aerial part of Polycline proteiformis were ground in a hammer mill, then extracted with ethanol by percolation for 24 hours at room temperature to give the crude extract N110635 (5 g), of which 3 g was shipped to Virginia Polytechnic Institute and
State University (VPISU) for further bioassay guided isolation. The fractionation tree is shown in Scheme 6.1. The extract N110635 [IC50: 1.9 μg/mL (HB3), 1.6 μg/mL (Dd2), 2 g] was suspended in aqueous MeOH (MeOH-H2O, 9:1, 100 mL) and extracted with hexanes (3 x 100 mL portions). The aqueous layer was then diluted to 60% MeOH (v/v) with H2O and extracted with CH2Cl2 (3 x 150 mL portions). The hexanes extract was evaporated in vacuo to leave 186.5 mg with an IC50 value of 4.0 (HB3), 2.0 (Dd2) μg/mL.
109
Scheme 6.1 Separation of ethanol extract of Polycline proteiformis
470.1 mg of residue from the CH2Cl2 extract had IC50 of 1.5 (HB3), <1.0 (Dd2) μg/mL.
The aqueous MeOH extract (1.392 g) was less active with IC50 of >10.0 (HB3), 8.0
(Dd2). Therefore, the CH2Cl2 extract was selected for fractionation by C18 open column, and four fractions were collected. Fractions I, II, III, and IV (155.7, 149.7, 54.2 and 19.3 mg) had IC50 values of > 5.0 (HB3), > 5.0 (Dd2); 0.45 (HB3), 0.42 (Dd2); 5.0 (HB3), 3.5
(Dd2); > 5.0 (HB3), > 5.0 (Dd2) μg/mL, respectively. Fraction II was selected for further separation by C18 preparative HPLC (60% CH3CN-H2O). Compound 6.1 (2.2 mg, tR 22.1
110 min), 6.2 (2.2 mg, tR 14.6 min), 6.3 (2.3 mg, tR 13.2 min), 6.4 (2.9 mg, tR 16.5 min), 6.5
(1.1 mg, tR 20.2 min) and 6.6 (2.3 mg, tR 10.0 min) were isolated.
6.3.3 Bioassays
Antiplasmodial assays with the chloroquine-sensitive strain P. falciparum HB3 and chloroquine-resistant strain P. falciparum Dd2 were performed by Professor Roepe and his group at Georgetown University. Both assays used the previously reported SYBR green 1 method.21 The A2780 ovarian cancer cell line assay was performed by Ms. Peggy
Brodie at Virginia Polytechnic Institute and State University as previously reported,22 except that the samples were added in 1 L 100% DMSO per well instead of 20 L of
23 1:1 DMSO:H2O. The A2780 cell line is a drug-sensitive ovarian cancer cell line.
6.3.4 Plant material
A sample of the aerial part of Polycline proteiformis Humbert (Asteraceae) was collected in April 24th, 1998 near Toliara, Madagascar. This aromatic herbaceous plant can grow up to 60 cm height, with white flowers. Voucher specimens have been deposited at Smithsonian Institution, Washington D.C..
(1S,2S,4R,5S,6S,7R,8S,10R)-2-isobutyryloxy-4-acetoxy-6-acetoxyguai-11(13)-en-8,12- olide (6.1, polyclinolide A)
23 Compound 6.1 was an off-white needle crystal; [α]D -53 (c 0.1, CHCl3); CD [θ]230 -
-1 3570 (MeOH); UV (MeOH) λmax nm (log ε) 211 (4.0); IR νmax cm : 3463, 2965, 1733,
-1 1 13 1464, 1375, 1258, 1156, 1052, 1018 cm . H NMR (500 MHz, CDCl3) and C NMR
111 + (125 MHz, CDCl3), see Table 6.1; HRESI-MS m/z 437.2176 [M+H] , (calcd for
C23H33O8 (437.2175).
X-ray Crystallography of 6.1
A colorless needle (0.03 x 0.03 x 0.33 mm3) of 6.1 was centered on the goniometer of an Oxford Diffraction SuperNova diffractometer operating with CuKα radiation. The data collection routine, unit cell refinement, and data processing were carried out with the program CrysAlisPro.24 The Laue symmetry and systematic absences were consistent with the monoclinic space groups C2, Cm, and C2/m. As the molecule was known to be enantiomerically pure, the chiral space group, C2, was chosen. The structure was solved using SHELXS-9725 and refined using SHELXL-9725 via OLEX2.26 The final refinement model involved anisotropic displacement parameters for non-hydrogen atoms and a riding model for all hydrogen atoms. The absolute configuration was established from anomalous dispersion effects [Flack x = 0.02(15);27 Hooft P2(true) = 1.000, P3(true) =
1.000, P3(rac-twin) = 0.3x10-5; P3(false) = 0.7x10-25, y = 0.06(9)].28,29 Crystal data were obtained by Dr. Carla Slebodnick: C23H32O8, Mr = 436.49, monocyclic, a = 32.7331(12)
Å, b = 7.1315(3) Å, c = 9.7799(3) Å, α = 90.00, β = 92.961, γ = 90.00, V = 2279.94(13)
Å3, 13543 reflections, 287 parameters. The atomic coordinates and equivalent isotropic displacement parameters, as well as a full list of bond distances and angles, and the structure factor table are deposited as supplementary material at the Cambridge
Crystallographic Data Centre (Deposition No. CCDC 802814).
112 (1S,2S,4R,5S,6R,7S,8S,10R)-2-isobutyryloxy-4-acetoxy-6-hydroxyguai-11(13)-en-8,12- olide (6.2, polyclinolide B)
23 Compound 6.2 was an off-white solid; [α]D -4 (c 0.2, CHCl3); CD [θ]230 -1560
-1 (MeOH); UV (MeOH) λmax nm (log ε) 211 (3.9); IR νmax cm : 3474, 2972, 1729, 1464,
-1 1 13 1376, 1250, 1160, 1044, 1017 cm . H NMR (500 MHz, CDCl3) and C NMR (125
+ MHz, CDCl3), see Table 6.1; HRESI-MS m/z 417.1889 [M+Na] , calcd for C21H30NaO7
(417.1889).
1α,7α,10βH-4-oxo-6α-angeloyloxy-8β-(3-hydroxy-3-methyl-butanoyloxy)pseudoguaia-
2(3),11(13)-dien-9β,12-olide (6.3, polyclinolide C)
23 Compound 6.3 was an off-white solid; [α]D -69 (c 0.1, CHCl3); UV (MeOH) λmax
-1 nm (log ε) 220 (4.2); IR νmax cm : 3444, 2924, 1721, 1458, 1382, 1229, 1154, 1035,
-1 1 13 999.8 cm . H NMR (500 MHz, CDCl3) and C NMR (125 MHz, CDCl3), see Table
+ 6.1; HRESI-MS m/z 483.2003 [M+Na] , calcd for C25H32NaO8 (483.1995).
1α,7α,10βH-4-oxo-6α-angeloyloxy-8β-(3-hydroxy-3-methyl-pentanoyloxy)pseudoguaia-
2(3),11(13)-dien-9β,12-olide (6.4, polyclinolide D)
23 Compound 6.4 was an off-white solid; [α]D -66 (c 0.2, CHCl3); UV (MeOH) λmax
-1 nm (log ε) 220 (4.3); IR νmax cm : 3445, 2927, 1721, 1458, 1382, 1216, 1154, 1035,
-1 1 13 999.5 cm . H NMR (500 MHz, CDCl3) and C NMR (125 MHz, CDCl3), see Table
+ 6.1; HRESI-MS m/z 497.2143 [M+Na] , calcd for C26H34NaO8 (497.2151) .
113 1α,7α,10βH-4-oxo-6α-(E)-3-methyl-2-pentenoyloxy-8β-(3-hydroxy-3-methyl- pentanoyloxy)pseudoguaia-2(3),11(13)-dien-9β,12-olide (6.5, polyclinolide E)
23 Compound 6.5 was an off-white solid; [α]D -33 (c 0.1, CHCl3); UV (MeOH) λmax
-1 nm (log ε) 221 (4.3); IR νmax cm : 3441, 2923, 1721, 1458, 1380, 1216, 1142, 1034,
-1 1 13 1005.5 cm . H NMR (500 MHz, CDCl3) and C NMR (125 MHz, CDCl3), see Table
+ 6.1; HRESI-MS m/z 489.2496 [M+H] , calcd for C27H37O8 (489.2488).
References
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117 VII. Miscellaneous Plants Studied
7.1 Introduction
During the search for novel anticancer and antimalarial agents, some extracts yielded only known compounds. These known compounds isolated from various species are reported in this chapter, to provide a complete record of the work that was done and to document the botanical sources of the isolated compounds.
7.2 Anticancer extracts
7.2.1 Chadsia racemosa (Fabaceae)
An ethanol extract of the leaves of Chadsia racemosa (Fabaceae) (MG 3288, 1.9 g) yielded 728 mg of an active dichloromethane fraction with an IC50 value of 11 μg/mL against the A2780 human ovarian cancer line after liquid/liquid partition. The most active subfraction with an IC50 value of 8 μg/mL was separated over a C18 open column followed by PTLC to afford the two known flavonoids 7.1 (2.0 mg) and 7.2 (1.2 mg) with
IC50 values of around 19 μM. Their structures were determined by mass spectrometry to deduce their molecular formulas and by comparion of their 1H and 13C data with literature data.1
118 7.2.2 Gastonia duplicata (Araliaceae)
An ethanol extract of the leaves of Gastonia duplicate (Araliaceae) (MG 4016) displayed an IC50 value of 4.2 μg/mL against the A2780 human ovarian cancer line.
Liquid/liquid partitioning of 110 mg of crude extract followed by separation of 82 mg of the bioactive methanol fraction with an IC50 value of 7.2 g/mL over a reversed phase
C18 open column, followed by HPLC on a C18 column to afford the two known bioactive
2 triterpenoid saponins 7.3 (1.3 mg) and 7.4 (4.2 mg), with IC50 values less than 1.7 μM.
The isolation work was done by Brian T. Murphy and the structure elucidation was done by Ende Pan.
7.2.3 Entada louvelii (Fabaceae)
Comparison of the antiproliferative activity of the crude extract (MG 3303) with the activities of the fractions from liquid/liquid partitioning of 490 mg crude extract indicated that this procedure did not improve activity. The most active BuOH fraction (293 mg) had an IC50 of 17 μg/mL. This fraction was fractionated over a C18 open column, followed by HPLC on a cyano column. The activities of the fractions remained around 15
μg/mL. Due to the low bioactivity of the fractions, further separation of the plant was discontinued.
7.2.4 Entada sp. (Fabaceae)
119 The crude bark extract of this unknown species (MG 2125) was active in the A2780 cell line at 14 μg/mL. Liquid/liquid partitioning of 693 mg crude extract yielded 202 mg active n-BuOH fraction with an IC50 of 11 μg/mL. This active fraction was further fractionated over C18 open column. The most active two fractions were active at approximately 6 μg/mL. Proton NMR spectroscopy indicated that there were two or three triterpene saponins in the active fractions. Since these types of compounds are not attractive drug candidates, further separation of the plant was discontinued.
7.2.5 Leea guineensis (Vitaceae)
The crude root extract of Leea guineensis (MG 1929) was shown to be active against the A2780 cell line at 19 μg/mL. Liquid/liquid partitioning of 74 mg of extract afforded four active fractions. The dichloromethane and the n-butanol fractions were found to be the most active at 15 μg/mL and 17 μg/mL, respectively. Since most of the weight of the sample was in the n-BuOH fraction, further separation work was performed on this fraction. After separation on Sephadex LH20, followed by C18 open column and C18
HPLC, the subfractions showed no interesting activity. Thus, further separation of the plant was discontinued.
7.2.6 Droceloncia reticulate (Euphorbiaceae)
Liquid/liquid partitioning of 3.3 g crude leaf extract of Droceloncia reticulate (MG
3736) led to three fractions. The dichloromethane fraction was subject to C18 open column and gave four sub-fractions. The most active fraction showed anitproliferative activity against 2780 cell line with an IC50 value about 4μg/mL. This fraction was
120 subjected to C18 HPLC to afford 14 fractions. All the fractions had IC50 values higher than 7 μg/mL, so the bioactivities had been lost by the separation. Further separation of this plant was thus discontinued.
7.3 Antimalarial extracts
7.3.1 Phyllanthus muellerianus (Euphorbiaceae)
An ethanol extract of the leaves of Phyllanthus muellerianus (Euphorbiaceae) was available from Dr. Patricia Onocha, a Fulbright scholar in the Kingston group from 2007-
2008. The bioassay work was carried out by Dr. Paul Roepe, Georgetown University.
The extract had IC50 values lower than 1 g/mL against the HB3 strain and 1.5 g/mL against the Dd2 strain. Liquid/liquid partitioning of 12 g of crude extract followed by separation of the active dichloromethane fraction (1.2 g) over reversed phase C18 open column, and C18 HPLC has so far afforded the five known compounds 7.5 (0.7 mg), 7.6
(0.8 mg), 7.7 (10. mg), 7.8 (4.3 mg), and 7.9 (1.0 mg). Only 7.9 showed encouraging activities with IC50 values about 2 against the HB3 and Dd2 malaria parasite strains.
121 An ethanol extract (10 g) of the roots of Phyllanthus muellerianus (Euphorbiaceae) displayed an IC50 value 2.2 g/mL against the Dd2 malaria parasite. The extract was separated by liquid-liquid partition to give an active dichloromethane fraction. This dichloromethane fraction was further separated on a C18 open column, and the most active subfraction (0.6g, IC50: < 1g/mL) was separated by C18 HPLC. Compounds 7.10
(0.6 mg) and 7.11 (0.8 mg) were isolated, but neither of them had any significant activity against the malaria parasite.
The structure determination of 7.10 and 7.11 was done by mass spectrometric analysis of their molecular formula and comparison of their 1H and 13C NMR data and their optical rotation data with literature data.3-6
The search for more potent antimalarial compounds from Phyllanthus muellerianus is being continued by other group members.
7.3.2 Microdesmis caseariifolia (Pandaceae)
122 An ethanol extract (100 mg) of the leaves of Microdemis caseariaefoli (Pandaceae)
(N015451) displayed IC50 values of 5.4 g/mL against the HB3 strain and 6.0 g/mL against the Dd2 strain of P. falciparum. Liquid/liquid partitioning of the crude extract followed by separation of 29 mg active dichloromethane fraction [IC50: 0.8 g/mL (HB3) and 0.4 g/mL (Dd2)] over reversed phase C18 open column, then by C18 HPLC afforded
5 1 10 1.0 mg of N -(p-coumaroyl)-N ,N -diferuloylspermidine 7.12 (keayanidine B) with IC50 >
1g/mL against both parasites. Its structure was confirmed by analysis of its 1H NMR data and the comparison of its mass spectrometric data with literature data.7
7.3.3 Majidea sp. (Sapindaceae)
Liquid/liquid partitioning of 116 mg crude leaf extract of Majidea sp. (MG 2164) led to 3 fractions. The most active methanol aqueous fraction with IC50 11 μg/mL against the
FCM29 malaria parasite was subjected to separation on C18 and Sephadex LH 20 open columns to give an active fraction (13.8 mg) with IC50 value about 4 μg/mL. The
123 subfractions of this fraction failed to produce any bioactivity lower than 7 μg/mL. Further separation of this plant thus was stopped.
7.3.4 Terminalia septentrionalis (Combretaceae)
An ethanol extract (113 mg) of the leaf extract of Terminalia septentrionalis (MG
2316) with an IC50 value of 18 μg/mL against the FCM29 malaria parasite was subjected to liquid-liquid partition to lead to three fractions. The most active aqueous methanol fraction (IC50 14 μg/mL, 53 mg) was separated by C18 open column chromatography and phenyl HPLC. None of the subfractions showed any increase in the bioactivity as compared with the aqueous methanol fraction. Further separation of this plant was discontinued.
7.4 Structure elucidation of compounds isolated in Madagascar
Dr. Vincent Rasamison at CNARP (Centre National des Recherches Pharmaceutique) in Madagascar is working on the isolation of potent antimalarial compounds. As part of the ICBG program, the isolated bioactive compounds were then sent to Virginia Tech for structure determination.
Twigs and leaves of Vitex cauliflora (Verbenaceae) were collected in the eastern region of Madagascar in 2005. One triterpenoid called uvaol (7.13) was isolated from the extract.8 Its structure was identified by comparison of its NMR data with literature values.9 It did not show interesting antimalarial activity.
Compounds 7.14-7.17 were isolated from the roots of Vepris macrophylla (Rutaceae), and were determined to be known compounds, with IC50 values 12 μg/mL, 12 μg/mL, 14
124 μg/mL and not active against the A2780 cell line, respectively. The structure of compound 7.14 was determined by comparing its spectroscopic data with literature values,10 and the structures of compounds 7.15-7.17 were determined by analysis of their
MS and NMR spectra.
References
1. Ngadjui, B. T.; Kouam, S. F.; Dongo, E.; Kapche, G. W. F.; Abegaz, B. M.,
Prenylated flavonoids from the aerial parts of Dorstenia mannii. Phytochemistry 2000,
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Bioorg. Med. Chem. 2009, 17, 3118-3125.
125 4. Wu, S. J.; Wu, T. S., Cytotoxic arylnaphthalene lignans from Phyllanthus
oligospermus. Chem. Pharm. Bull. 2006, 54, 1223-1225.
5. Gonzalez, A. G.; Darias, V.; Alonso, G., Cytostatic lignans isolated from
Haplophyllum hispanicum. Planta Med. 1979, 36, 200-203.
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aurantiacum. Phytochemistry 1981, 20, 2217-2220.
7. Zamble, A.; Sahpaz, S.; Hennebelle, T.; Carato, P.; Bailleul, F., N-1,N-5,N-10-tris(4-
hydroxycinnamoyl)spermidines from Microdesmis keayana roots. Chem. Biodiversity
2006, 3, 982-989.
8. Rasamison, V. E.; Ranaivo-Harimanana, L.; Cao, S.; Pan, E.; Ratovoson, F.;
Randriantafika, F.; Rakotondrajaona, R.; Rakotonandrasana, S.; Andriantsiferana, R.;
Kingston, D. G. I., A new labdane diterpene from Vitex cauliflora Moldenke from the
Madagascar rainforest. Fitoterapia 2010, 81, 55-58.
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The secondary metabolites of aff. Samadera SAC-2825: An Australian
Simaroubaceae with unusual chemistry. Phytochemistry 1997, 44, 1109-1114.
126 VIII. General Conclusions
In our continuing search for biologically active natural products from tropical rainforests as part of an International Cooperative Biodiversity Group (ICBG) program, more than fifteen plants were selected for initial isolation. Six of them were further fractionated to yield twelve new and ten known compounds under the guidance of the
A2780 human ovarian cancer cell line. Four antimalarial extracts were selected for further separation to give five new and eight known compounds.
8.1 Anticancer extracts
Fractionation of the leaves, fruit and inflorescence of Symphonia tanenlesis led to the isolation of one novel xanthone and two known guttiferones. The xanthone showed moderate activity with IC50 3.8 μM , and both guttiferones also showed moderate activity against the A2780 cancer cell line with IC50 values of 8.3 μM and 7.8 μM.
Two new compounds were isolated from the roots of Bussea sakalava, four of which are diphenyl propanes. The other compound has a cycloheptadibenzofuran skeleton which has not been previously reported. A possible biosynthetic pathway of the cycloheptadibenzofuran was proposed.
Fractionation of the roots of Leptadenia madagascariensis led to four new cardenolide glycosides, all of which showed strong antiproliferative activity against
A2780 human ovarian cancer cell line (IC50: 0.18, 0.21, 0.17 and 0.29 M) and the H460 lung cancer cell line (IC50: 0.16, 0.68, 0.37 and 0.48 M).
127 Four known and two new alkaloids were isolated from the roots of Ambavia gerradii.
The structures of the new alkaloids were confirmed by total synthesis. Among these alkaloids, the known alkaloid sampagine showed the strongest antiproliferative activities against the A2780 cell line (IC50: 0.60 M) and H460 cell line (IC50:0.58 M).
8.2 Antimalarial extracts
Fractionation of aerial parts of Polycline proteiformis guided by assay against HB3 and Dd2 malaria parasites led to the isolation and structure elucidation of five new sesquiterpene lactones called pseudoguaianolides and the flavanoid centaureidin. Two of those new pseudoguaianolide displayed strong antimalarial activities against both parasites with IC50 values < 1.0 M. All of the pseudoguaianolides showed strong antiproliferative activities against the A2780 cell line. However, they are thus not good lead compounds for antimalarial drug development.
Five known compounds were isolated from the leaves of Phyllanthus muellerianus, none of which showed strong activities against HB3 and Dd2 malaria parasite, although the crude extract was tested to have strong activities against both malaria strains. Some other fractions were found to have strong antimalarial activities. Time did not permit the isolation of pure compounds from these fractions, and it is recommended that further exploration of this plant be undertaken to isolate potent antimalarial compounds.
128 APPENDIX (1H and 13C NMR spectra)
1 H NMR spectrum of 2.1 in CD3OD
13 C NMR spectrum of 2.1 in CD3OD
129 1 H NMR spectrum of 3.1 in CD3OD
13 C NMR spectrum of 3.1 in CD3OD
130 1 H NMR spectrum of 3.2 in CD3OD
13 C NMR spectrum of 3.2 in CD3OD
131 1 H NMR spectrum of 3.3 in CD3OD
13 C NMR spectrum of 3.3 in CD3OD
132 1 H NMR spectrum of 3.4 in CD3OD
13 C NMR spectrum of 3.4 in CD3OD
133 1 H NMR spectrum of 3.5 in CDCl3
13 C NMR spectrum of 3.5 in CDCl3
134 1H NMR spectrum of 4.1 in d-Pyridine
13C NMR spectrum of 4.1 in d-Pyridine
135 1 H NMR spectrum of 4.2 in CD3OD
13 C NMR spectrum of 4.2 in CD3OD
136 1H NMR spectrum of 4.3 in d-Pyridine
13C NMR spectrum of 4.3 in d-Pyridine
137 1 H NMR spectrum of 4.3 in CD3OD
13 C NMR spectrum of 4.3 in CD3OD
138 1 H NMR spectrum of 4.4 in CD3OD
13 C NMR spectrum of 4.4 in CD3OD
139 1 H NMR spectrum of 5.1 in CD3OD
13 C NMR spectrum of 5.1 in CD3OD
140 1 H NMR spectrum of 5.2 in CD3OD
13 C NMR spectrum of 5.2 in CD3OD
141 1 H NMR spectrum of 5.11 in CD3OD
13 C NMR spectrum of 5.11 in CD3OD
142 1 H NMR spectrum of 5.3 in CDCl3
1 H NMR spectrum of 5.4 in CDCl3
143 1 H NMR spectrum of 5.5 in CDCl3
1 H NMR spectrum of 5.6 in CDCl3
1 H NMR spectrum of 6.1 in CDCl3
144
13 C NMR spectrum of 6.1 in CDCl3
145 1 H NMR spectrum of 6.2 in CDCl3
13 C NMR spectrum of 6.2 in CDCl3
1 H NMR spectrum of 6.3 in CDCl3
146
13 C NMR spectrum of 6.3 in CDCl3
1 H NMR spectrum of 6.4 in CDCl3
147
13 C NMR spectrum of 6.4 in CDCl3
1 H NMR spectrum of 6.5 in CDCl3
148
13 C NMR spectrum of 6.5 in CDCl3
1 H NMR spectrum of 6.6 in CD3OD
149
13 C NMR spectrum of 6.6 in CD3OD
150