Meeting of the ERFP WG "Ex situ 19/06/2019 conservation"

Portuguese Animal Germplasm Bank animal genetic resources

Rosa Lino Neto Pereira

Unit of Biotechnology and Genetic Resources Estação Zootécnica Nacional, Santarém Portugal [email protected]

ERFP the Ex situ Working Group - Madrid 2019 May

Portugal has a rich and diverse animal genetic heritage with 62

autochthonous Burro de Miranda

breeds Bisaro

Several of the Pedrês domestic animal Garrano 62 Preta de Montesinho autochthonous Serra da Estrela breeds of Portugal Malhado de Alcobaça are currently in an Barbado da Terceira endangered status Churra do Campo

Garvonesa Barrocal Algarvio

Madrid, May 22nd 2019 1 Meeting of the ERFP WG "Ex situ 19/06/2019 conservation"

North Portuguese Animal Center Germplasm Bank

South

ERFP the Ex situ Working Group - Madrid 2019 May

Semen

Cattle Sheep Goats Bordaleira ED Algarvia Algarvia Minho Bravia Embryos Campaniça Charnequeira Barrosã Churra Algarvia Preta de Breed N♀ Total Brava Churra Badana Montesinho Ch Galega 6 20 Churra do Campo Serpentina Churra do Minho Serrana (3 Jarmelista Chur G Mirandesa ecotipos) Mer Prta 29 161 ChuG Brangançana 6 breeds, 59 Algarvia 7 41 Merina Branca animais, 12588 Serpentina 14 93 Merina da Beira doses Baixa Bravia 2 10 Mirandesa Merina Preta Serrana 4 41 Preta Mondegueira Alentejana 10 51 Saloia Pigs and Brava Serra da Estrela Mertolenga 2 9 horses Ovarian tissue 15 breeds, 235 14 breeds, 92 1 breed each animals, 175 834 animals, 12842 Algarvia 3 6 ov doses dosesadapt Horta et al. 2012 ERFP

Madrid, May 22nd 2019 2 Meeting of the ERFP WG "Ex situ 19/06/2019 conservation"

DNA

 DNA samples from all animals integrating BPGA Blood and hair samples

Analysis of molecular markers to estimate intra- and inter-racial genetic variability and to measure genetic distances between breeds were done to perform their characterization

ERFP the Ex situ Working Group - Madrid 2019 May

DGAV and INIAV joint mission

 Principal goal – to ensure the recovery and maintenance of germplasm from all Portuguese breeds: • Gamete • Embryos • Somatic cells • DNA To guarantee its long-term conservation, in accordance with the recommendations of the competent international organizations

ERFP the Ex situ Working Group - Madrid 2019 May

Madrid, May 22nd 2019 3 Meeting of the ERFP WG "Ex situ 19/06/2019 conservation"

DGAV and INIAV joint mission

 Guarantee their availability for use in the selection and conservation programs of the Portuguese breeds, according to the norms established in the BPGA rules Promote the exchange of information and, when appropriate, genetic material with foreign counterparts

ERFP the Ex situ Working Group - Madrid 2019 May

Portuguese Animal Germplasm Bank

Sept 2010

Semen and Working Embryos Genetic Storage Reservoir Centre

Nuclear BPGA Committee genetic reservoir  2 INIAV

 2 DGAV

 3 from breeders associations

BPGA

ERFP the Ex situ Working Group - Madrid 2019 May

Madrid, May 22nd 2019 4 Meeting of the ERFP WG "Ex situ 19/06/2019 conservation"

Semen from donors standing at a semen collection Centre approved by the competent authority in accordance with Directive 88/407/EEC  Embryos must be collected and processed by approved and supervised embryo collection teams Donors health status must ensure that the risk of spreading animal disease is eliminated Conservation proposes

ERFP the Ex situ Working Group - Madrid 2019 May

Madrid, May 22nd 2019 5 Meeting of the ERFP WG "Ex situ 19/06/2019 conservation"

Portuguese Breeds

Cattle Horses Chicken Ovinos Pigs Dogs Goats DonKeys Algarvia Burro de Bord . Entre Douro Cão de Castro 1 Alentejana Amarela Alentejana Miranda e Minho Laboreiro Algarvia Burro da Cão da Serra da 2 Bravia Branca Campaniça Bisara Graciosa Estrela Arouquesa Charnequeira Pedrês Malhado de 3 Garrana Churra Algarvia Rafeiro do Alentejo Portuguesa Alcobaça Barrosã Preta Preta Cão de Fila de S. 4 Lusitana Churra Badana Montesinho Lusitânica Miguel Brava de Lide Churra da Terra Cão da Serra da 5 Serrana Sorraia Quente Aires Brava dos Serpentina Poneida 6 Churra do Campo Podengo Português Açores Terceira Cachena Perdigueiro 7 Churra do Minho Português Churra Galega Garvonesa Cão de Água 8 Bragançana Português Branca Jarmelista Churra Galega Cão de Gado 9 Bragançana Preta Transmontano Marinhoa Churra Galega Barbado da 10 Mirandesa Terceira Churra Cão do Barrocal 11 Maronesa Mondegueira Algarvio 12 Mertolenga Merina Branca

13 Minhota Merina Preta Merino da Beira 14 Mirandesa Baixa Preta 15 Saloia

16 Ramo Grande Serra da Estrela

Madrid, May 22nd 2019 6