Antimicrobial Effects of Silver Nanoparticles Student Lab Activity

Total Page：16

File Type：pdf, Size：1020Kb

Antimicrobial Effects of Silver Nanoparticles Student Lab Activity Purpose: This lab was developed to test claims that colloidal silver has anti-microbial effects. Many products are on the market that have nanoparticles of silver in them. These include socks, clothing and food containers. One of their advertising claims is that the silver nanoparticles inhibit the growth of bacteria and hence reduce odors and/or spoilage. Materials: 1 mM silver nitrate Scissors 38.8 mM (1%) sodium citrate Tweezers Hot plate E. coli bacteria culture Small test tubes Filter paper 250 mL beaker Q-tip swabs Disposable transfer pipets Incubator Agar plates Small plastic petri dishes Procedure: 1. Make colloidal silver using the following method: a. Add 2 mL of silver nitrate to a small test tube. b. Place this test tube in a 250 mL beaker of hot water. c. Leave in the beaker of hot water for about 10 minutes. d. Add 7 drops of sodium citrate to the test tube containing hot silver nitrate. e. Continue to heat until the silver nitrate solution turns color (yellowish). f. Remove test tubes and set in a test tube rack to cool. 2. Cut filter paper into small squares. 3. Place filter paper squares in a small petri dish and pour the test tube of colloidal silver over the squares. 4. Let the filter paper squares soak for about 10 minutes (can be even longer). 5. While waiting for the filter paper to soak, mark the bottom of a petri plate with initials, divide the bottom into sections and label each of the sections. Remember to set up control for comparison. 6. Use a 1 mL disposable transfer pipet and spread 1 drop of the bacteria culture on the agar plate using a Q-tip swab. 7. After 10 minutes of soaking, place filter paper squares in the designated areas. 8. Incubate the petri plates overnight at 37 degrees Celsius. 9. On the second day of this activity, the petri plates should be removed from the incubator and examined. Copyright © 2012 Board of Trustees, University of Illinois. All rights reserved. University of Illinois • Caltech • Stanford University • North Carolina A&T Analyze Data: 1. Draw your petri plate; be sure to include your labels. 2. Describe the bacterial growth in each labeled section. 3. How do you explain any differences that you observe? University of Illinois • Caltech • Stanford University • North Carolina A&T Questions: 1. Are products containing silver nanoparticles justified in claiming that these products have antimicrobial effects? 2. What conclusions can you draw about the effect of silver nanoparticles to bacterial cell growth based on these images? SEM images of Healthy E. coli (left) and E. coli cultured in the presence of silver nanoparticles (right) University of Illinois • Caltech • Stanford University • North Carolina A&T .

Copy Link

Recommended publications

Microbiology Laboratory Exercises Third Edition 2020
MICROBIOLOGY Laboratory Exercises Third Edition Keddis & Rauschenbach 2020 Photo Credits (in order of contribution): Diane Davis, Ines Rauschenbach & Ramaydalis Keddis Acknowledgements: Many thanks to those in the Department of Biochemistry and Microbiology, Rutgers University, who have through the years inspired our enthusiasm for the science and teaching of microbiology, with special thanks to Diane Davis, Douglas Eveleigh and Max Häggblom. Safety: The experiments included in this manual have been deemed safe by the authors when all necessary safety precautions are met. The authors recommend maintaining biosafety level 2 in the laboratory setting and using risk level 1 organisms for all exercises. License: This work is licensed under a Creative Commons Attribution- NonCommercial-NoDerivatives 4.0 International License Microbiology Laboratory Exercises Third Edition 2020 Ramaydalis Keddis, Ph.D. Ines Rauschenbach, Ph.D. Department of Biochemistry and Microbiology Rutgers, The State University of New Jersey CONTENTS PAGE Introduction Schedule ii Best Laboratory Practices Iii Working in a Microbiology Laboratory iv Exercises Preparation of a Culture Medium 1 Culturing and Handling Microorganisms 3 Isolation of a Pure Culture 5 Counting Bacterial Populations 8 Controlling Microorganisms 10 Disinfectants 10 Antimicrobial Agents: Susceptibility Testing 12 Hand Washing 14 The Lethal Effects of Ultraviolet Light 15 Selection of Fungi from Air 17 Microscopy 21 Morphology and Staining of Bacteria 26 Microbial Metabolism 30 Enzyme Assay 32 Metabolic
[Show full text]
Page 1 of 27 the Diagram Shows Two Thermometers. the Bulb of Each
Q1. The diagram shows two thermometers. The bulb of each thermometer is covered with a piece of wet cotton wool. One of the thermometers is placed in the draught from a fan. The graph shows how the temperature of each thermometer changes with time. (a) Which of the graph lines, A or B, shows the temperature of the thermometer placed in the draught? Write the correct answer in the box. Page 1 of 27 Explain, in terms of evaporation, the reason for your answer. ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ (3) (b) A wet towel spread out and hung outside on a day without wind dries faster than an identical wet towel left rolled up in a plastic bag. Explain why. ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ ___________________________________________________________________ (2) (Total 5 marks) Q2. The picture shows a person taking a hot shower. (a) When a person uses the shower the mirror gets misty. Why? ___________________________________________________________________ ___________________________________________________________________
[Show full text]
Determination of the Identity of an Unknown Liquid Group # My Name the Date My Period Partner #1 Name Partner #2 Name
Determination of the Identity of an unknown liquid Group # My Name The date My period Partner #1 name Partner #2 name Purpose: The purpose of this lab is to determine the identity of an unknown liquid by measuring its density, melting point, boiling point, and solubility in both water and alcohol, and then comparing the results to the values for known substances. Procedure: 1) Density determination Obtain a 10mL sample of the unknown liquid using a graduated cylinder Determine the mass of the 10mL sample Save the sample for further use 2) Melting point determination Set up an ice bath using a 600mL beaker Obtain a ~5mL sample of the unknown liquid in a clean dry test tube Place a thermometer in the test tube with the sample Place the test tube in the ice water bath Watch for signs of crystallization, noting the temperature of the sample when it occurs Save the sample for further use 3) Boiling point determination Set up a hot water bath using a 250mL beaker Begin heating the water in the beaker Obtain a ~10mL sample of the unknown in a clean, dry test tube Add a boiling stone to the test tube with the unknown Open the computer interface software, using a graph and digit display Place the temperature sensor in the test tube so it is in the unknown liquid Record the temperature of the sample in the test tube using the computer interface Watch for signs of boiling, noting the temperature of the unknown Dispose of the sample in the assigned waste container 4) Solubility determination Obtain two small (~1mL) samples of the unknown in two small test tubes Add an equal amount of deionized into one of the samples Add an equal amount of ethanol into the other Mix both samples thoroughly Compare the samples for solubility Dispose of the samples in the assigned waste container Observations: The unknown is a clear, colorless liquid.
[Show full text]
Maintenance of Bacterial Strains
Maintenance of Bacterial Strains Short-term growth and maintenance The simplest method for obtaining strains is to order them from a microbiological supply company. Presque Isle, Ward, and Carolina Biological (see “Resources” page of the Microbes Web site: www.umsl.edu/~microbes) are economical sources of strains that are commonly used in the classroom. Another possible source is a local college or university that teaches a microbiology lab course. If they have strains already prepared for their class, they will probably be willing to provide you with a culture. This is probably not a reasonable ongoing source of strains, but can be useful in an emergency. For short-term maintenance and use, it is best to streak bacteria on some type of rich agar petri plate, such as nutrient agar. Label and date the plate on the bottom of the plate. Streak from the source culture using good sterile technique. The quadrant streak method (see the article on quadrant streaking) is good for obtaining well-isolated colonies. Incubate the plate only until good colonies have formed. Do not leave the culture in the incubator beyond that time, or the cells will die on the plate. Store the plate in the refrigerator. It will keep for about a month. For class use, it is best to streak a fresh plate 2-3 days before the strain will be needed for class. Use the fresh plate as the source of cultures for the class. For broth cultures for a class, it is best to grow a larger volume culture in a flask (if possible, with shaking) overnight and then dispense aliquots of the master culture into sterile test tubes using a sterile pipette.
[Show full text]
Pouring Plates from Prepared Bottled Media
Pouring Plates from Prepared Bottled Media Primary Hazard Warning Never purchase living specimens without having a disposition strategy in place. When pouring bottles, agar is HOT! Burning can occur. Always handle hot agar bottles with heat-protective gloves. For added protection wear latex or nitrile gloves when working with bacteria, and always wash hands before and after with hot water and soap. Availability Agar is available for purchase year round. Information • Storage: Bottled agar can be stored at room temperature for about six months unless otherwise specified. Never put agar in the freezer. It will cause the agar to breakdown and become unusable. To prevent contamination keep all bottles and Petri dishes sealed until ready to use. • Pouring Plates • Materials Needed: • Draft-free enclosure or Laminar flow hood • 70% isopropyl alcohol • Petri dishes • Microwave or hot water bath or autoclave 1. Melt the agar using one of the following methods: a) Autoclave: Loosen the cap on the agar bottle and autoclave the bottle at 15 psi for five minutes. While wearing heat-protective gloves, carefully remove the hot bottle and let it cool to between 75–55°C before pouring. This takes approximately 15 minutes. b)Water Bath: Loosen the cap on the agar bottle and place it into a water bath. Water temperature should remain at around 100°C. Leave it in the water bath until the agar is completely melted. While wearing heat- protective gloves, carefully remove the hot bottle and let it cool to between 75–55°C before pouring. c) Microwave: Loosen the cap on the agar bottle before microwaving.
[Show full text]
Microlab® STAR™
Microlab ® STAR™ Microlab ® STAR ™ AUTOMATED WORKFLOW SOLUTIONS CENTERED AROUND YOUR ASSAY The STAR combines Hamilton's patented pipetting technology including precise lock-and-key tip attachment, unrivaled liquid level detection, and comprehensive volume ranges to create flexible liquid handling workstations. Available in three base platform sizes, the STAR portfolio incorporates countless options to automate your workflows. Hamilton Robotics has also partnered with top leaders in the biotechnology industry to provide Standard Solutions based on commonly automated applications. Offering ready-to-start protocols for a variety of applications such as NGS, ELISA, and forensic assays, our Standard Solutions provide a faster way to automate your processes. 2 1 PATENTED TECHNOLOGY The STAR utilizes Hamilton’s proprietary Compressed O-Ring Expansion (CO-RE®) technology. CO-RE minimizes the production of aerosols and allows disposable tips or washable, steel needles to be used on channels in the same run. 2 MULTI-FUNCTIONAL ARM Our technology offers high pipetting accuracy and precision, from sub-microliter to large volumes, using Independent Channels and/or the Multi-Probe Head (MPH). Labware transportation is possible with the iSWAP® or CO-RE Grippers. The STAR can incorporate a camera, tube transportation, and other channel tools on a single arm. Comprehensive pipetting range: ■■■0.5 μL to 1 mL using the 1 mL Independent Channel ■■■50 μL to 5 mL using the 5 mL Independent Channel ■■■1 μL to 1 mL using the CO-RE 96 MPH ■■■0.1 μL to 50 μL using the CO-RE 384 MPH 3 FLEXIBLE SETUP The high-capacity deck is customized specific to your workflow, accommodating a wide range of labware and automated devices that can easily be exchanged to support multiple assays on one platform.
[Show full text]
Laboratory Equipment Reference Sheet
Laboratory Equipment Stirring Rod: Reference Sheet: Iron Ring: Description: Glass rod. Uses: To stir combinations; To use in pouring liquids. Evaporating Dish: Description: Iron ring with a screw fastener; Several Sizes Uses: To fasten to the ring stand as a support for an apparatus Description: Porcelain dish. Buret Clamp/Test Tube Clamp: Uses: As a container for small amounts of liquids being evaporated. Glass Plate: Description: Metal clamp with a screw fastener, swivel and lock nut, adjusting screw, and a curved clamp. Uses: To hold an apparatus; May be fastened to a ring stand. Mortar and Pestle: Description: Thick glass. Uses: Many uses; Should not be heated Description: Heavy porcelain dish with a grinder. Watch Glass: Uses: To grind chemicals to a powder. Spatula: Description: Curved glass. Uses: May be used as a beaker cover; May be used in evaporating very small amounts of Description: Made of metal or porcelain. liquid. Uses: To transfer solid chemicals in weighing. Funnel: Triangular File: Description: Metal file with three cutting edges. Uses: To scratch glass or file. Rubber Connector: Description: Glass or plastic. Uses: To hold filter paper; May be used in pouring Description: Short length of tubing. Medicine Dropper: Uses: To connect parts of an apparatus. Pinch Clamp: Description: Glass tip with a rubber bulb. Uses: To transfer small amounts of liquid. Forceps: Description: Metal clamp with finger grips. Uses: To clamp a rubber connector. Test Tube Rack: Description: Metal Uses: To pick up or hold small objects. Beaker: Description: Rack; May be wood, metal, or plastic. Uses: To hold test tubes in an upright position.
[Show full text]
LOWERING COSTS and SIMPLIFYING WORKFLOW with the Automate 2550 SYSTEM
HACETTEPE UNIVERSITY FACULTY OF MEDICINE HOSPITAL CENTRAL AND STAT LABORATORIES LOWERING COSTS AND SIMPLIFYING WORKFLOW WITH THE AutoMate 2550 SYSTEM Laboratory profile › 1,040-bed hospital located in Ankara, Turkey › Provides uninterrupted 24/7 inpatient, outpatient and emergency services › Employs 6 professors, 6 associate Hacettepe University Faculty of Medicine Hospital is one of the leading professors and 130 operators university hospitals in Turkey. The university is well known for its › Performs up to 6.5 million geographical location and adoption of ground breaking technologies. biochemistry and hormone While the reference site is preferred by a large number of patients, it is tests annually of particular interest to specific patient populations seeking test results › Processes up to 2 million tests from a variety of panels. With many different systems performing on instruments in more than hundreds of standard and specialized tests, the hospital’s laboratories 20 different units are driven to ensure the delivery of results without error, while › Operates a Beckman Coulter containing costs and managing workload burden. standalone AutoMate 2550 pre- For more than 16 years, the facility—which has been held up as an and post-analytical automation system in the biochemistry example of excellence in its own region, as well as all over the country— laboratory, along with a Power has been using a standalone automation system with an aliquoter Processor system with two feature. With this technology, it has enhanced patient satisfaction,
[Show full text]
Chapter 2 2.1 Description of Kit Items
Manual of SEAT Friendly MCL Kit 7 Chapter 2 2.1 Description of Kit Items S.No. Item Name Figure/Setup Uses (Quantity/ kit) 1. Storage box opens on both sides. Contains necessary 1. Wooden box apparatus for doing with revolving experiments. top 2. Revolving top containing (1) dispensing bottles and vials for easy access to chemicals. 1. As a container for keeping liquids. 2. Microbeaker (10 mL) (12) 1. As a container for use in electrochemical experiments, 3. Beaker (50 thermochemistry experiments mL) preparation of salts, etc. (3) Steps for use 1. Bore the rubber cork to fit the stem of Hirsch funnel. 2. Cut the filter paper to fit the mesh of the funnel. The size should exactly fit the mesh, 4. Micro- neither big nor small. filtration 3. Fit the cork with funnel in unit the mouth of the boiling tube (2) (having a side tube). 4. Transfer the solution to be filtrated to the funnel. 5. Press the bulb with hand to evacuate it and place it on the side tube of the boiling tube while keeping it pressed. 6. Release the pressure. The bulb will suck the air inside the boiling tube allowing fast filtration. 8 Manual of SEAT Friendly MCL Kit S.No. Item Name Figure/Setup Uses (Quantity/Kit) 1. The watch glass is used to hold solids when being 5. Watch glass weighed. (2) 2. It should never be heated. 1. Flat plate with multiple “wells” used as micro test tubes. 2. Contains 96 rectangular matrix. 3. Each well of a micro plate typically holds somewhere between a few to a few hundred microlitres of liquid.
[Show full text]
Laboratory Supplies and Equipment
Laboratory Supplies and Equipment Beakers: 9 - 12 • Beakers with Handles • Printed Square Ratio Beakers • Griffin Style Molded Beakers • Tapered PP, PMP & PTFE Beakers • Heatable PTFE Beakers Bottles: 17 - 32 • Plastic Laboratory Bottles • Rectangular & Square Bottles Heatable PTFE Beakers Page 12 • Tamper Evident Plastic Bottles • Concertina Collapsible Bottle • Plastic Dispensing Bottles NEW Straight-Side Containers • Plastic Wash Bottles PETE with White PP Closures • PTFE Bottle Pourers Page 39 Containers: 38 - 42 • Screw Cap Plastic Jars & Containers • Snap Cap Plastic Jars & Containers • Hinged Lid Plastic Containers • Dispensing Plastic Containers • Graduated Plastic Containers • Disposable Plastic Containers Cylinders: 45 - 48 • Clear Plastic Cylinder, PMP • Translucent Plastic Cylinder, PP • Short Form Plastic Cylinder, PP • Four Liter Plastic Cylinder, PP NEW Polycarbonate Graduated Bottles with PP Closures Page 21 • Certified Plastic Cylinder, PMP • Hydrometer Jar, PP • Conical Shape Plastic Cylinder, PP Disposal Boxes: 54 - 55 • Bio-bin Waste Disposal Containers • Glass Disposal Boxes • Burn-upTM Bins • Plastic Recycling Boxes • Non-Hazardous Disposal Boxes Printed Cylinders Page 47 Drying Racks: 55 - 56 • Kartell Plastic Drying Rack, High Impact PS • Dynalon Mega-Peg Plastic Drying Rack • Azlon Epoxy Coated Drying Rack • Plastic Draining Baskets • Custom Size Drying Racks Available Burn-upTM Bins Page 54 Dynalon® Labware Table of Contents and Introduction ® Dynalon Labware, a leading wholesaler of plastic lab supplies throughout
[Show full text]
ED149972.Pdf
-74 ..t.rcrVtr.,,,.. r . ", 0 ' 4^Az7 .. ' . DOCUMNIT ant= 2D 149 972 SE 023- 413 AUTHOR . We'sterhold, Arnold F.., Ed.; 'Bennett; Ernest C., . Ed. , _ TITLE : Ahanual of Simplified Laboratory Methods for . Operators of 'Wastewater Treatment Facilities.. INSTITUTION' Illinois State Epvircidental.Protection Agencyc , .. - 'Springfield.. PUB DATE Apr 74 NOTE 94p.; Pages 1-1 through 1-12 (General Introduction) removed due to copyright restrictions; Section 8 'missing; Contains occasional light type; Best Copy / Available EDRS PRICE .M7-$0.83 HC-$4.67 Plus Postage. DESCRIPTORS, Chemistry; Environmental Education; *Environmental Technicians; Independent Study; *Instructional Materials; Job Skills; *Laboratory Tchniques; *Pollution; *Post Seccndary Education; Public Health; *Water Pollution Control IDENTIFIERS cf*Waste Water Treatment ABSTRACT . ./ This manual is designed to provide the seall wastewater treatment plant operator, as well as the new or inexperienced operator, with simplified methods for laboratory analysis of'water"and wastewater. It is emphasized that this manual is not a replacement for standard methods but a guide for plantswith insufficient equipment to pefform analyses ifi..accordance with ,standard methods. Eacj, of the sections is designed to becomplete I within itself. The tests and measurements 'presented include: acids, biochethical oxygen demand (BOD);40674plved oxygen, residues, sludge, a'nd suspended solids. (CS) el. -V or 4. ***********44*************************************i*******i*******4S*** I4 * Reproductions
[Show full text]
High School Chemistry
RECOMMENDED MINIMUM CORE INVENTORY TO SUPPORT STANDARDS-BASED INSTRUCTION HIGH SCHOOL GRADES SCIENCES High School Chemistry Quantity per Quantity per lab classroom/ Description group adjacent work area SAFETY EQUIPMENT 2 Acid storage cabinet (one reserved exclusively for nitric acid) 1 Chemical spill kit 1 Chemical storage reference book 5 Chemical waste containers (Categories: corrosives, flammables, oxidizers, air/water reactive, toxic) 1 Emergency shower 1 Eye wash station 1 Fire blanket 1 Fire extinguisher 1 First aid kit 1 Flammables cabinet 1 Fume hood 1/student Goggles 1 Goggles sanitizer (holds 36 pairs of goggles) 1/student Lab aprons COMPUTER ASSISTED LEARNING 1 Television or digital projector 1 VGA Adapters for various digital devices EQUIPMENT/SUPPLIES 1 box Aluminum foil 100 Assorted rubber stoppers 1 Balance, analytical (0.001g precision) 5 Balance, electronic or manual (0.01g precision) 1 pkg of 50 Balloons, latex 4 Beakers, 50 mL 4 Beakers, 100 mL 2 Beakers, 250 mL Developed by California Science Teachers Association to support the implementation of the California Next Generation Science Standards. Approved by the CSTA Board of Directors November 17, 2015. Quantity per Quantity per lab classroom/ Description group adjacent work area 2 Beakers, 400 or 600 mL 1 Beakers, 1000 mL 1 Beaker tongs 1 Bell jar 4 Bottle, carboy round, LDPE 10 L 4 Bottle, carboy round, LDPE 4 L 10 Bottle, narrow mouth, 1000 mL 20 Bottle, narrow mouth, 125 mL 20 Bottle, narrow mouth, 250 mL 20 Bottle, narrow mouth, 500 mL 10 Bottle, wide mouth, 125
[Show full text]