Microbiology. Applied to Protecting the Line
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Determination of the Identity of an Unknown Liquid Group # My Name the Date My Period Partner #1 Name Partner #2 Name
Determination of the Identity of an unknown liquid Group # My Name The date My period Partner #1 name Partner #2 name Purpose: The purpose of this lab is to determine the identity of an unknown liquid by measuring its density, melting point, boiling point, and solubility in both water and alcohol, and then comparing the results to the values for known substances. Procedure: 1) Density determination Obtain a 10mL sample of the unknown liquid using a graduated cylinder Determine the mass of the 10mL sample Save the sample for further use 2) Melting point determination Set up an ice bath using a 600mL beaker Obtain a ~5mL sample of the unknown liquid in a clean dry test tube Place a thermometer in the test tube with the sample Place the test tube in the ice water bath Watch for signs of crystallization, noting the temperature of the sample when it occurs Save the sample for further use 3) Boiling point determination Set up a hot water bath using a 250mL beaker Begin heating the water in the beaker Obtain a ~10mL sample of the unknown in a clean, dry test tube Add a boiling stone to the test tube with the unknown Open the computer interface software, using a graph and digit display Place the temperature sensor in the test tube so it is in the unknown liquid Record the temperature of the sample in the test tube using the computer interface Watch for signs of boiling, noting the temperature of the unknown Dispose of the sample in the assigned waste container 4) Solubility determination Obtain two small (~1mL) samples of the unknown in two small test tubes Add an equal amount of deionized into one of the samples Add an equal amount of ethanol into the other Mix both samples thoroughly Compare the samples for solubility Dispose of the samples in the assigned waste container Observations: The unknown is a clear, colorless liquid. -
METHODS & STANDARD OPERATING PROCEDURES (Sops)
METHODS & STANDARD OPERATING PROCEDURES (SOPs) OF EMISSION TESTING IN HAZARDOUS WASTE INCINERATOR FOREWARD COVR PAGE TEAM CHAPTER TITLE PAGE NO Chapter - 1 Stack Monitoring – Material And Methodology For Isokinetic Sampling 1-28 Chapter - 2 Standard Operating Procedure (SOP) For Particulate Matter Determination 29-38 Chapter - 3 Determination Of Hydrogen Halides (Hx) And Halogens From Source Emission 39-54 Chapter - 4 Standard Operating Procedure For The Sampling Of Hydrogen Halides And Halogens From Source Emission 55-61 Determination Of Metals And Non Metals Emissions From Chapter - 5 Stationarysources 62-79 Standard Operating Procedure (Sop) For Sampling Of Metals And Non Chapter - 6 Metals & 80-97 Standard Operating Procedure (Sop) Of Sample Preparation For Analysis Of Metals And Non Metals Chapter - 7 Determination Of Polychlorinated Dibenzo-P-Dioxins And 98-127 Polychlorinated Dibenzofurans Standard Operating Procedure For Sampling Of Polychlorinated Chapter - 8 Dibenzo-P-Dioxins And Polychlorinated Dibenzofurans & 128-156 Standard Operating Procedure For Analysis Of Polychlorinated Dibenzo- P-Dioxins And Polychlorinated Dibenzofurans Project Team 1. Dr. B. Sengupta, Member Secretary - Overall supervision 2. Dr. S.D. Makhijani, Director - Report Editing 3. Sh. N.K. Verma, Ex Additional Director - Project co-ordination 4. Sh. P.M. Ansari, Additional Director - Report Finalisation 5. Dr. D.D. Basu, Senior Scientist - Guidance and supervision 6. Sh. Paritosh Kumar, Sr. Env. Engineer - Report processing 7. Sh. Dinabandhu Gouda, Env. Engineer - Data compilation, analysis and preparation of final report 8. Sh. G. Thirumurthy, AEE - - do - 9. Sh. Abhijit Pathak, SSA - - do - 10. Ms. Trapti Dubey, Jr. Professional (Scientist) - - do - 11. Er. Purushotham, Jr. Professional (Engineer) - - do - 12. -
Laboratory Equipment Reference Sheet
Laboratory Equipment Stirring Rod: Reference Sheet: Iron Ring: Description: Glass rod. Uses: To stir combinations; To use in pouring liquids. Evaporating Dish: Description: Iron ring with a screw fastener; Several Sizes Uses: To fasten to the ring stand as a support for an apparatus Description: Porcelain dish. Buret Clamp/Test Tube Clamp: Uses: As a container for small amounts of liquids being evaporated. Glass Plate: Description: Metal clamp with a screw fastener, swivel and lock nut, adjusting screw, and a curved clamp. Uses: To hold an apparatus; May be fastened to a ring stand. Mortar and Pestle: Description: Thick glass. Uses: Many uses; Should not be heated Description: Heavy porcelain dish with a grinder. Watch Glass: Uses: To grind chemicals to a powder. Spatula: Description: Curved glass. Uses: May be used as a beaker cover; May be used in evaporating very small amounts of Description: Made of metal or porcelain. liquid. Uses: To transfer solid chemicals in weighing. Funnel: Triangular File: Description: Metal file with three cutting edges. Uses: To scratch glass or file. Rubber Connector: Description: Glass or plastic. Uses: To hold filter paper; May be used in pouring Description: Short length of tubing. Medicine Dropper: Uses: To connect parts of an apparatus. Pinch Clamp: Description: Glass tip with a rubber bulb. Uses: To transfer small amounts of liquid. Forceps: Description: Metal clamp with finger grips. Uses: To clamp a rubber connector. Test Tube Rack: Description: Metal Uses: To pick up or hold small objects. Beaker: Description: Rack; May be wood, metal, or plastic. Uses: To hold test tubes in an upright position. -
Eight New Filter Bags from Whirl-Pak® Three New Hydrated Sponge
NEW PRODUCTS Eight new filter bags from whirl-pak® Specially designed for use with homogenizer blenders. Extra-heavy PET film and special features for easier and more efficient testing. Additional sizes and styles on page 11. Vertical Filter Bags Bags use a special laminated PET film for durability and a vertical nonwoven filter to separate solids for easy pipetting of the liquid sample. Available with or without a closure, each bag contains a 250 micron polyester filter adhered vertically along the side of the 3 mil (0.76 mm) bag. Easy-pour side seal allows you to simply pour liquid out of the bag without also emptying solids. Hydrated PolySponge™ Bags B01586 — 55 oz. With Closure three new Hydrated sponge products from whirl-pak® Make environmental surface sampling faster and easier with these pre-moistened bags. Durable high-density polyurethane sponge is ready to use and resists tearing and fraying. Each is hydrated with HiCap™ Neutralizing Broth. Additional information on page 12. Hydrated PolyProbe™ HiCap™ is a registered trademark of World Bioproducts, LLC. ctured under a ufa qua n li Whirl-Pak® Bags are manufactured under a quality management system certified to ISO9001, except B01299, B01350, a t y M m o , , , , , , , and which require an additional processing step an t B01392 B01422 B01423 B01475 B01478 B01590 B01591 B01592 age fied ISO9001ment system certi done outside of regular production. • Standard Methods Says — Standard Methods (18th edition, 1992, section 9060A, sample containers) states “For some applications, samples may be collected in pre-sterilized plastic bags.” Whirl-Pak® bags meet this description. -
LOWERING COSTS and SIMPLIFYING WORKFLOW with the Automate 2550 SYSTEM
HACETTEPE UNIVERSITY FACULTY OF MEDICINE HOSPITAL CENTRAL AND STAT LABORATORIES LOWERING COSTS AND SIMPLIFYING WORKFLOW WITH THE AutoMate 2550 SYSTEM Laboratory profile › 1,040-bed hospital located in Ankara, Turkey › Provides uninterrupted 24/7 inpatient, outpatient and emergency services › Employs 6 professors, 6 associate Hacettepe University Faculty of Medicine Hospital is one of the leading professors and 130 operators university hospitals in Turkey. The university is well known for its › Performs up to 6.5 million geographical location and adoption of ground breaking technologies. biochemistry and hormone While the reference site is preferred by a large number of patients, it is tests annually of particular interest to specific patient populations seeking test results › Processes up to 2 million tests from a variety of panels. With many different systems performing on instruments in more than hundreds of standard and specialized tests, the hospital’s laboratories 20 different units are driven to ensure the delivery of results without error, while › Operates a Beckman Coulter containing costs and managing workload burden. standalone AutoMate 2550 pre- For more than 16 years, the facility—which has been held up as an and post-analytical automation system in the biochemistry example of excellence in its own region, as well as all over the country— laboratory, along with a Power has been using a standalone automation system with an aliquoter Processor system with two feature. With this technology, it has enhanced patient satisfaction, -
Chapter 2 2.1 Description of Kit Items
Manual of SEAT Friendly MCL Kit 7 Chapter 2 2.1 Description of Kit Items S.No. Item Name Figure/Setup Uses (Quantity/ kit) 1. Storage box opens on both sides. Contains necessary 1. Wooden box apparatus for doing with revolving experiments. top 2. Revolving top containing (1) dispensing bottles and vials for easy access to chemicals. 1. As a container for keeping liquids. 2. Microbeaker (10 mL) (12) 1. As a container for use in electrochemical experiments, 3. Beaker (50 thermochemistry experiments mL) preparation of salts, etc. (3) Steps for use 1. Bore the rubber cork to fit the stem of Hirsch funnel. 2. Cut the filter paper to fit the mesh of the funnel. The size should exactly fit the mesh, 4. Micro- neither big nor small. filtration 3. Fit the cork with funnel in unit the mouth of the boiling tube (2) (having a side tube). 4. Transfer the solution to be filtrated to the funnel. 5. Press the bulb with hand to evacuate it and place it on the side tube of the boiling tube while keeping it pressed. 6. Release the pressure. The bulb will suck the air inside the boiling tube allowing fast filtration. 8 Manual of SEAT Friendly MCL Kit S.No. Item Name Figure/Setup Uses (Quantity/Kit) 1. The watch glass is used to hold solids when being 5. Watch glass weighed. (2) 2. It should never be heated. 1. Flat plate with multiple “wells” used as micro test tubes. 2. Contains 96 rectangular matrix. 3. Each well of a micro plate typically holds somewhere between a few to a few hundred microlitres of liquid. -
Laboratory Supplies and Equipment
Laboratory Supplies and Equipment Beakers: 9 - 12 • Beakers with Handles • Printed Square Ratio Beakers • Griffin Style Molded Beakers • Tapered PP, PMP & PTFE Beakers • Heatable PTFE Beakers Bottles: 17 - 32 • Plastic Laboratory Bottles • Rectangular & Square Bottles Heatable PTFE Beakers Page 12 • Tamper Evident Plastic Bottles • Concertina Collapsible Bottle • Plastic Dispensing Bottles NEW Straight-Side Containers • Plastic Wash Bottles PETE with White PP Closures • PTFE Bottle Pourers Page 39 Containers: 38 - 42 • Screw Cap Plastic Jars & Containers • Snap Cap Plastic Jars & Containers • Hinged Lid Plastic Containers • Dispensing Plastic Containers • Graduated Plastic Containers • Disposable Plastic Containers Cylinders: 45 - 48 • Clear Plastic Cylinder, PMP • Translucent Plastic Cylinder, PP • Short Form Plastic Cylinder, PP • Four Liter Plastic Cylinder, PP NEW Polycarbonate Graduated Bottles with PP Closures Page 21 • Certified Plastic Cylinder, PMP • Hydrometer Jar, PP • Conical Shape Plastic Cylinder, PP Disposal Boxes: 54 - 55 • Bio-bin Waste Disposal Containers • Glass Disposal Boxes • Burn-upTM Bins • Plastic Recycling Boxes • Non-Hazardous Disposal Boxes Printed Cylinders Page 47 Drying Racks: 55 - 56 • Kartell Plastic Drying Rack, High Impact PS • Dynalon Mega-Peg Plastic Drying Rack • Azlon Epoxy Coated Drying Rack • Plastic Draining Baskets • Custom Size Drying Racks Available Burn-upTM Bins Page 54 Dynalon® Labware Table of Contents and Introduction ® Dynalon Labware, a leading wholesaler of plastic lab supplies throughout -
Protecting Consumers. Improving Lab Efficiency. 3M™ Petrifilm™ Plates and Reader Simply Brilliant
Protecting Consumers. Improving lab efficiency. 3M™ Petrifilm™ Plates and Reader Simply brilliant. This red dot changed microbiology. Imagine what it can do for your lab. In today’s environment of heightened focus on food safety and increasingly stringent quality requirements, you want your lab to provide tests that are fast, reliable and consistently accurate. For more than 30 years, food safety professionals around the world have put their trust in 3M™ P e t r i fi l m ™ Plates. That’s because these ingenious “little red dots” have streamlined, standarised and simplifi ed the process of microbial quantitative indicator testing, improving productivity and helping ensure the highest levels of product quality. Simply effi cient. Maximise productivity Improve consistency Plates are sample-ready, eliminating the time-consuming, cumbersome step of Compared to the variability associated with agar preparation, 3M’s preparing agar dishes. Plus, plates such as the E.coli/Coliform Count and Staph standardised formula improves consistency across technicians, shifts and Express Count plates go even further by eliminating the need for a subsequent plants – worldwide. Each lot of Petrifi lm plates goes through rigorous quality confi rmation step required with traditional agar methods. In a study conducted testing at our ISO 9001 certifi ed manufacturing facility. This reduces the need with 292 food-processing plants, companies increased their QA personnel for lot qualifi cations in operations making their own agar. More than 200 effi ciency by an average of 80.5%, saving an average of 3.7 hours per day of evaluations from peer-review publications and validating agencies worldwide technician time by using 3M™ Petrifi lm™ Plates. -
High School Chemistry
RECOMMENDED MINIMUM CORE INVENTORY TO SUPPORT STANDARDS-BASED INSTRUCTION HIGH SCHOOL GRADES SCIENCES High School Chemistry Quantity per Quantity per lab classroom/ Description group adjacent work area SAFETY EQUIPMENT 2 Acid storage cabinet (one reserved exclusively for nitric acid) 1 Chemical spill kit 1 Chemical storage reference book 5 Chemical waste containers (Categories: corrosives, flammables, oxidizers, air/water reactive, toxic) 1 Emergency shower 1 Eye wash station 1 Fire blanket 1 Fire extinguisher 1 First aid kit 1 Flammables cabinet 1 Fume hood 1/student Goggles 1 Goggles sanitizer (holds 36 pairs of goggles) 1/student Lab aprons COMPUTER ASSISTED LEARNING 1 Television or digital projector 1 VGA Adapters for various digital devices EQUIPMENT/SUPPLIES 1 box Aluminum foil 100 Assorted rubber stoppers 1 Balance, analytical (0.001g precision) 5 Balance, electronic or manual (0.01g precision) 1 pkg of 50 Balloons, latex 4 Beakers, 50 mL 4 Beakers, 100 mL 2 Beakers, 250 mL Developed by California Science Teachers Association to support the implementation of the California Next Generation Science Standards. Approved by the CSTA Board of Directors November 17, 2015. Quantity per Quantity per lab classroom/ Description group adjacent work area 2 Beakers, 400 or 600 mL 1 Beakers, 1000 mL 1 Beaker tongs 1 Bell jar 4 Bottle, carboy round, LDPE 10 L 4 Bottle, carboy round, LDPE 4 L 10 Bottle, narrow mouth, 1000 mL 20 Bottle, narrow mouth, 125 mL 20 Bottle, narrow mouth, 250 mL 20 Bottle, narrow mouth, 500 mL 10 Bottle, wide mouth, 125 -
Manual on Laboratory Testing of Fishery Products
ISSN: 1995-4875 CRFM Special Publication. No.14 Manual on Laboratory Testing of Fishery Products The SPS Project is funded by the European Union under the 10th Economic Development Fund and is being implemented by the Inter-American Institute for Cooperation on Agriculture (IICA) with the following regional Partners: the CARICOM Secretariat, the Caribbean Regional Fisheries Mechanism (CRFM), El Comité Nacional para la Aplicació n de Medidas Sanitarias y Fitosanitarias de la Repú blica Dominicana (CNMSF) and CARIFORUM. Manual on Laboratory Testing of Fisheries Products Copyright © 2016 by Caribbean Regional Fisheries Mechanism (CRFM) All rights reserved. Reproduction, dissemination and use of material in this publication for educational or non- commercial purposes are authorized without prior written permission of the CRFM, provided the source is fully acknowledged. No part of this publication may be reproduced, disseminated or used for any commercial purposes or resold without the prior written permission of the CRFM. Prepared by: Christine Froese, Megapesca Lda., December 2016, under contract to the Inter- American Institute for Cooperation on Agriculture (IICA), through the 10th EDF funded Sanitary and Phytosanitary Project Correct Citation: Froese, C, 2016. Manual on Laboratory Testing of Fishery Products. CRFM Special Publication. No.14. 89pp. ISSN: 1995-4875 ISBN: 978-976-8257-38-3 Cover Photo: Fishery Product Testing Laboratory, Saint Vincent Contents 1 INTRODUCTION ............................................................................................................................... -
Next Generation Microbiology for the Future
Next Generation Microbiology for the Future www.msk.or.kr | 1 2014 INTERNATIONAL MEETING of the MICROBIOLOGICAL SOCIETY of KOREA 2 | 2014 International Meeting of the Microbiological Society of Korea 2014 INTERNATIONAL MEETING of Next Generationthe MICROBIOLOGICAL Microbiology for the Future SOCIETY of KOREA Contents • Timetable ············································································································································ 4 • Floor Plan ··········································································································································· 5 • Scientific Programs ···························································································································· 6 • Plenary Lectures······························································································································· 23 PL1 ······································································································································· 24 PL2 ······································································································································· 25 PL3 ······································································································································· 26 PL4 ······································································································································· 27 • Symposia ·········································································································································· -
When Magnesium Metal Reacts with Hydrochloric Acid, Hydrogen Gas Is Produced
Clements—Chemistry I H Molar Volume of a Gas When magnesium metal reacts with hydrochloric acid, hydrogen gas is produced. The volume of this gas can be measured by using a eudiometer. A eudiometer is a giant test tube that has graduated volume measurements. Knowing the number of moles of magnesium used, you can calculate the volume of hydrogen produced per mole of magnesium consumed. The balanced equation for this reaction also allows you to determine the volume that one mole of gas occupies as a recorded temperature and pressure. Procedure Half fill a tall cylinder with tap water, and place about 1O0mL of tap water in a 25O-mL beaker. Obtain ~1OmL of 3M hydrochloric acid in a graduated cylinder. Acquire a piece of magnesium ribbon and measure and record its mass to the nearest 0.OOlg. Roll the magnesium ribbon into a loose coil. Tie the coil with a piece of thread in such a manner that all of the loops of the coil are tied together. With a funnel, carefully pour ~10mL of 3M HCl (aq) into the eudiometer. Using the room temperature water in the 250-mL beaker, very slowly pour water from the beaker into the eudiometer which is in a slightly tipped position. Be careful to layer the water over the acid so that they do not mix thoroughly. Add enough water to fill the eudiometer completely. Using the thread, lower the magnesium coil into the water in the eudiometer to a depth of ~5cm. Insert the rubber stopper into the mouth of the eudiometer to hold the thread in position.