Laboratory Supplies and Equipment
Total Page:16
File Type:pdf, Size:1020Kb
Load more
Recommended publications
-
PP003: Large Scale Growth of M. Tuberculosis
SOP: PP003.2 Modified: 6/10/2010 Large-scale growth of Mycobacterium tuberculosis Materials and Reagents: 1. Biosafety cabinet (BSC) 2. Three 100 ml frozen stocks of M. tuberculosis (see SOP PP002) 3. Eight 2.8 L glass fernbach flasks containing 900 ml sterile GAS medium, capped with cotton and cheesecloth plugs wrapped in aluminum foil 4. Orbital platform shaker 5. Four 50 ml disposable pipets 6. Electric pipetter 7. Bunsen burner 8. Pipet boat 9. 10% Lysol I. C. solution 10. Autoclave 11. Forty 1 liter rollerbottles containing 400 ml sterile GAS medium 12. Two 25 ml disposable pipets 13. Rollerbottle apparatus 14. Rubbermaid transport cart 15. Vacuum pump 16. Five 4 liter Winchester bottles, sterile 17. Five 0.2 um Zap Cap S plus bottle filtration units 18. 225 ml Falcon centrifuge bottle 19. 225 ml centrifuge bottle rack 20. Harvard trip balance 21. Sorvall benchtop centrifuge 22. Sorvall benchtop centrifuge rotor, containing 225 ml centrifuge bottle hanging buckets 23. Sterile Milli-Q water 24. 4oC cold room (BHRB Molecular Biology room 101) 25. –80oC freezer (option, note 17) Upscale protocol: (note 1) 1. _____ Inside a biosafety cabinet in a BSL-3 facility, thaw three “100 ml” stocks of M. tuberculosis. 2. _____ Carefully pour the contents of one stock into one 2.8 L fernbach flask containing GAS medium. 3. _____ Flame opening and aseptically replace foil and cotton plug in mouth of the fernbach flask. 4. _____ Repeat for two remaining “100 ml” stocks. 5. _____ Place the inoculated fernbach flasks on an orbital platform shaker at 37oC for two weeks (notes 2 and 3). -
Zinc and Cadmium in Paper (Reaffirmation of T 438 Cm-96)
WI 050114.01 T 438 DRAFT NO. 5 DATE July 27, 2006 TAPPI WORKING GROUP CHAIRMAN J Ishley SUBJECT CATEGORY Fillers & Pigments Testing RELATED METHODS See “Additional Information” CAUTION: This Test Method may include safety precautions which are believed to be appropriate at the time of publication of the method. The intent of these is to alert the user of the method to safety issues related to such use. The user is responsible for determining that the safety precautions are complete and are appropriate to their use of the method, and for ensuring that suitable safety practices have not changed since publication of the method. This method may require the use, disposal, or both, of chemicals which may present serious health hazards to humans. Procedures for the handling of such substances are set forth on Material Safety Data Sheets which must be developed by all manufacturers and importers of potentially hazardous chemicals and maintained by all distributors of potentially hazardous chemicals. Prior to the use of this method, the user must determine whether any of the chemicals to be used or disposed of are potentially hazardous and, if so, must follow strictly the procedures specified by both the manufacturer, as well as local, state, and federal authorities for safe use and disposal of these chemicals. Zinc and cadmium in paper (Reaffirmation of T 438 cm-96) (no changes were made since last draft) 1. Scope and significance 1.1 This method maybe used for the determination of cadmium and zinc either in paper or in highly opaque pigments. Zinc is usually present in zinc oxide, zinc sulfide, or as lithopone (a combination of zinc sulfide and barium sulfate), which is occasionally used in filled paper, in paper coatings and in high-pressure laminates and wallpaper. -
Laboratory Glassware N Edition No
Laboratory Glassware n Edition No. 2 n Index Introduction 3 Ground joint glassware 13 Volumetric glassware 53 General laboratory glassware 65 Alphabetical index 76 Índice alfabético 77 Index Reference index 78 [email protected] Scharlau has been in the scientific glassware business for over 15 years Until now Scharlab S.L. had limited its sales to the Spanish market. However, now, coinciding with the inauguration of the new workshop next to our warehouse in Sentmenat, we are ready to export our scientific glassware to other countries. Standard and made to order Products for which there is regular demand are produced in larger Scharlau glassware quantities and then stocked for almost immediate supply. Other products are either manufactured directly from glass tubing or are constructed from a number of semi-finished products. Quality Even today, scientific glassblowing remains a highly skilled hand craft and the quality of glassware depends on the skill of each blower. Careful selection of the raw glass ensures that our final products are free from imperfections such as air lines, scratches and stones. You will be able to judge for yourself the workmanship of our glassware products. Safety All our glassware is annealed and made stress free to avoid breakage. Fax: +34 93 715 67 25 Scharlab The Lab Sourcing Group 3 www.scharlab.com Glassware Scharlau glassware is made from borosilicate glass that meets the specifications of the following standards: BS ISO 3585, DIN 12217 Type 3.3 Borosilicate glass ASTM E-438 Type 1 Class A Borosilicate glass US Pharmacopoeia Type 1 Borosilicate glass European Pharmacopoeia Type 1 Glass The typical chemical composition of our borosilicate glass is as follows: O Si 2 81% B2O3 13% Na2O 4% Al2O3 2% Glass is an inorganic substance that on cooling becomes rigid without crystallising and therefore it has no melting point as such. -
Detection of Acid-Producing Bacteria Nachweis Von Säureproduzierenden Bakterien Détection De Bactéries Produisant Des Acides
(19) TZZ ¥ _T (11) EP 2 443 249 B1 (12) EUROPEAN PATENT SPECIFICATION (45) Date of publication and mention (51) Int Cl.: of the grant of the patent: C12Q 1/04 (2006.01) G01N 33/84 (2006.01) 19.11.2014 Bulletin 2014/47 (86) International application number: (21) Application number: 10790013.6 PCT/US2010/038569 (22) Date of filing: 15.06.2010 (87) International publication number: WO 2010/147918 (23.12.2010 Gazette 2010/51) (54) DETECTION OF ACID-PRODUCING BACTERIA NACHWEIS VON SÄUREPRODUZIERENDEN BAKTERIEN DÉTECTION DE BACTÉRIES PRODUISANT DES ACIDES (84) Designated Contracting States: (74) Representative: Isarpatent AL AT BE BG CH CY CZ DE DK EE ES FI FR GB Patent- und Rechtsanwälte GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO Friedrichstrasse 31 PL PT RO SE SI SK SM TR 80801 München (DE) (30) Priority: 15.06.2009 US 187107 P (56) References cited: 15.03.2010 US 314140 P US-A- 4 528 269 US-A- 5 098 832 US-A- 5 164 301 US-A- 5 601 998 (43) Date of publication of application: US-A- 5 601 998 US-A- 5 786 167 25.04.2012 Bulletin 2012/17 US-B2- 6 756 225 US-B2- 7 150 977 (73) Proprietor: 3M Innovative Properties Company • DARUKARADHYA J ET AL: "Selective Saint Paul, MN 55133-3427 (US) enumeration of Lactobacillus acidophilus, Bifidobacterium spp., starter lactic acid bacteria (72) Inventors: and non-starter lactic acid bacteria from Cheddar • YOUNG, Robert, F. cheese", INTERNATIONAL DAIRY JOURNAL, Saint Paul, Minnesota 55133-3427 (US) ELSEVIER APPLIED SCIENCE, BARKING, GB, • MACH, Patrick, A. -
Optimized Handling. the New Generation of Wet Stone Grinders
Optimized Handling. The new generation of wet stone grinders. Wet stone grinder FLEX and natural stone. A special relationship. Natural stone has its own laws. That call for special experts. Specialists who know how to read the stone. Who by the nuances and shades of the colours in the stone can recognise the unique masterpiece that a completely sculptured piece of work can evolve, but to realize this vision good tools are needed. For over 80 years FLEX is the competent and innovative partner for working with natural stone. Over this course of time a comprehensive range of machines has been developed in close co-operation with experienced stone masons. A product range that sets the industry standards for robustness and reliability also with handling and maintenance but especially by working safety when using water. The long-time competence of FLEX also includes the knowledge that FLEX engineers have gained about the nature of stone, its characteristics, and its sensitivities. That is why a FLEX machine of course does not damage or soil the valuable “natural stone”, but professionally processes and preserves it. As in every perfect relationship allows you to work to perfection. Wet stone grinder The FLEX PRCD safety switch ensures electrical safety when undertaking wet sanding. Is released practi- cally immediately (< 15 milliseconds)at the required response sensitivity. Complete with accidental restart safeguard on the power being interrupted. The contour plug is standard equipment on many wet grinders. It can be plugged only into the isolation transformer and not into the power outlet. The extensive FLEX natural stone range provides everything - from pressurized water tank, isolating transformers and safety vacuum cleaners to grinding discs, abrasive rings and diamond tools. -
Standard Operating Procedures
Standard Operating Procedures 1 Standard Operating Procedures OVERVIEW In the following laboratory exercises you will be introduced to some of the glassware and tech- niques used by chemists to isolate components from natural or synthetic mixtures and to purify the individual compounds and characterize them by determining some of their physical proper- ties. While working collaboratively with your group members you will become acquainted with: a) Volumetric glassware b) Liquid-liquid extraction apparatus c) Distillation apparatus OBJECTIVES After finishing these sessions and reporting your results to your mentor, you should be able to: • Prepare solutions of exact concentrations • Separate liquid-liquid mixtures • Purify compounds by recrystallization • Separate mixtures by simple and fractional distillation 2 EXPERIMENT 1 Glassware Calibration, Primary and Secondary Standards, and Manual Titrations PART 1. Volumetric Glassware Calibration Volumetric glassware is used to either contain or deliver liquids at a specified temperature. Glassware manufacturers indicate this by inscribing on the volumetric ware the initials TC (to contain) or TD (to deliver) along with the calibration temperature, which is usually 20°C1. Volumetric glassware must be scrupulously clean before use. The presence of streaks or droplets is an indication of the presence of a grease film. To eliminate grease from glassware, scrub with detergent solution, rinse with tap water, and finally rinse with a small portion of distilled water. Volumetric flasks (TC) A volumetric flask has a large round bottom with only one graduation mark positioned on the long narrow neck. Graduation Mark Stopper The position of the mark facilitates the accurate and precise reading of the meniscus. If the flask is used to prepare a solution starting with a solid compound, add small amounts of sol- vent until the entire solid dissolves. -
Q Path Adhesive Slide
In Vitro Diagnostic Medical Device For professional use only Q PATH ADHESIVE SLIDE According to standard ISO 18113-2 : 2009, Point 7 Requirements for instructions or use and consolidated Directive 98/79/EC PRODUCT NAME Cat. No Description Pack Size 10048001 Q path Adhesive Slide white Box of 72 slides 10048002 Q path Adhesive Slide blue Box of 72 slides 10048003 Q path Adhesive Slide green Box of 72 slides 10048004 Q path Adhesive Slide yellow Box of 72 slides 10048005 Q path Adhesive Slide pink Box of 72 slides INTENDED PURPOSE These microscope slides may be used for the routine diagnosis of suspensions of cells and samples of tissue. Given that the possibilities for their use are highly varied, these glass slides should only be handled by users who hold a professional qualification that complies with national legislation. These microscope slides are intended for single use only. Any re-use or inappropriate treatment of their surface could lead to inaccurate results, the deterioration of preparations, and erroneous diagnoses. WARNING AND PRECAUTIONS Before use, please read all this information carefully. Microscope slides and cover slips are intended for single use by qualified personnel. They must under no circumstances be used more than once, and must be disposed of after use or at the end of the archive period in an appropriate manner as potentially infectious waste. If you observe any damage to the packaging, or any signs of glass fragments, the slides and cover slips must not be used, due to the risk of injury from glass splinters. Recommendation: In order to reduce the risk of injury due to sharp edges as much as possible, we strongly recommend the use of our microscope slides with polished edges. -
7. Heating and Cooling Technology Contents
GENERAL CATALOGUE EDITION 18 7. Heating and cooling technology Contents Cooling 730 Refrigerators and Freezers ex protected 730 Refrigerators and Freezers 733 Low and Ultra low temperature freezers 737 Ice machines 744 Cryogenic tubes 746 Cryogenic boxes 749 Cryogenic racks 756 Cryogenic accessories 757 Transport boxes 759 Dewar flasks 760 Cryogenic storage tanks 763 Liquid nitrogen-Accessories 766 Temperature regulators 767 Heating-Thermostats 767 Cooling-Thermostats 779 Circulator baths 787 Dynamic Temperature Control Systems 791 Temperature Control Accessories 794 Heating 798 Water baths 798 Shaking water baths 805 Heating baths 810 Microwaves 814 Universal, Heating and Drying incubators 815 Vacuum drying incubators 819 Incubators 821 Cooling incubators 825 CO2-Incubators 829 Shaking incubators 833 Test incubators 847 Ovens, Furnaces 854 Hotplates 858 Incubation hoods 863 Temperature controllers, thermostats 868 729 www.wenk-labtec.com 7. Heating and cooling technology GENERAL CATALOGUE EDITION 18 Cooling/Refrigerators and Freezers ex protected 1 Spark-free laboratory refrigerators The BGR 120 laboratory guidelines state that interiors of refrigerators in which AQUALYTIC dangerous, explosive atmospheres can develop, must be spark-free. Such explosive atmospheres can be created by stored flammable liquids, for example. The laboratory refrigerators by Aqualytic® meet these requirements. The interiors are free from sparking sources and are therefore explosion-proof. With glass shelves. Variable temperature control from +2°C to +10°C. Temperature is continuously controlled by thermostat. Capacity External Internal Weight Power Temp. PK Cat. No. dimensions dimensions range (W x D x H) (W x D x H) LmmmmkgW°C 150 602 x 600 x 885 515 x 443 x 700 37.00 120 +2 .. -
Funnels and Filters
51 FUNNELS AND FILTERS Buchner Funnels 260MM (10.25") INSIDE DIAMETER, 203MM (8") OVERALL HEIGHT, 127MM (5") RIM TO PLATE CATALOG NO. DESCRIPTION PRICE/EACH H14620-0000 Funnel with Coarse Porosity Fixed Plate $216.00 H14620-1260 Funnel with Coarse Porosity Removable Plate 216.00 H14625-3510 Funnel with Medium Porosity Fixed Plate 216.00 H14626-3510 Funnel with Medium Porosity Removable Plate 216.00 H14627-0000 Funnel with Perforated Fixed Plate 216.00 Table-Top H14627-1260 Funnel with Perforated Removable Plate 216.00 Buchner 457MM (18") INSIDE DIAMETER, 292MM (11.5") OVERALL HEIGHT, 203MM (8") RIM TO PLATE Funnels CATALOG NO. DESCRIPTION PRICE/EACH With Fritware® Porous H14621-0000 Funnel with Coarse Porosity Fixed Plate $540.00 Filter or HDPE H14621-1457 Funnel with Coarse Porosity Removable Plate 540.00 H14625-3518 Funnel with Medium Porosity Fixed Plate 540.00 Perforated Plate H14626-3518 Funnel with Medium Porosity Removable Plate 540.00 Polyethylene funnels are H14628-0000 Funnel with Perforated Fixed Plate 465.00 one piece design with a H14627-1457 Funnel with Perforated Removable Plate 465.00 FIXED or REMOVABLE Custom sizes available upon request. 610MM (24") INSIDE DIAMETER, 330MM (13") OVERALL HEIGHT, 267MM (10.25") RIM TO PLATE porous filter plate CATALOG NO. DESCRIPTION PRICE/EACH supported by a multiple ring grid. Filter plates are made of high density H14622-0000 Funnel with Coarse Porosity Fixed Plate $1125.00 1 polyethylene 6.4mm ( ⁄4") thick with a non-porous ring at the periphery of the plate H14622-1610 Funnel with Coarse Porosity Removable Plate 1125.00 1 H14625-3524 Funnel with Medium Porosity Fixed Plate 1125.00 which seals filter paper when used. -
Federal Register/Vol. 73, No. 121/Monday, June 23, 2008/Notices
Federal Register / Vol. 73, No. 121 / Monday, June 23, 2008 / Notices 35395 specific injury research that focuses on originally published in the Federal DATES: Written comments concerning prevention and control. Register in 1999 (64 FR 14086), ‘‘Notice the change in the volume of liquid in Matters to be Discussed: The meeting will Regarding Requirement for Annual the pH determination and the addition include the review, discussion, and Submission of the Quantity of Nicotine of four commercial smokeless tobacco evaluation of individual research grant and Contained in Smokeless Tobacco product categories must be received on cooperative agreement applications submitted in response to Fiscal Year 2008 Products Manufactured, Imported, or or before July 23, 2008. Requests for Applications related to the Packaged in the United States,’’ and ADDRESSES: Comments should be following individual research announcement: revised in the Federal Register on marked ‘‘Comments on Revised Protocol ‘‘Elimination of Health Disparities Through March 14, 2008 (73 FR 13903), for Analysis of Nicotine’’ and mailed to Translation Research (R18), Request for implements the requirement of the the Centers for Disease Control and Application (RFA) CD08–001 for the National Comprehensive Smokeless Tobacco Prevention, National Center for Chronic Center for Environmental Health Health Education Act (CSTHEA) of 1986 Disease Prevention and Health Applications.’’ (15 U.S.C. 4401 et seq., Pub. L. 99–252) Promotion, Office on Smoking and Agenda items are subject to change as that each entity manufacturing, priorities dictate. Health, Attention: Matthew McKenna, Contact Person for More Information: Jane packaging, or importing smokeless M.D., Director, 4770 Buford Highway Suen, PhD, M.S., Executive Secretary, NCIPC tobacco products shall annually provide NE., MS K–50, Atlanta, Georgia 30341– IRG, CDC, 4770 Buford Highway, NE., M/S the Secretary of Health and Human 3724. -
SC-300 Pro Kit Manual 1 Version 2.3D Materials Provided in the Kit
Vinmetrica SC-300 Pro Kit™ User Manual Vinmetrica SC-300 Pro Kit is a simple and robust device that provides high accuracy in determination of sulfite (SO2), pH and titratable acidity (TA) levels in wines, ciders, and other liquids. These are essential parameters to control in the effort to make high quality wines. The Pro kit includes lab accessories for the SC-300 Analyzer. Table of Contents: Materials Provided in the Kit............................................................................................................... 2 Things you will need............................................................................................................................ 2 Why Test for SO2, pH and TA?............................................................................................................ 3 Theory of Operation............................................................................................................................. 4 Setup.................................................................................................................................................... 5 Setting up the SC-300 for the first time................................................................................... 5 Assembling the Pro Kit Equipment......................................................................................... 6 Burette Maintenance................................................................................................................ 8 Burette Reading ...................................................................................................................... -
Glass Container Styles
Glass Container Styles Glass Bottles & Jars Boston Round Bottles Boston Round Bottles are general use bottles that are perfect for liquids, product storage, and field or plant sampling projects. They feature a round body, rounded shoulders and narrow screw neck opening. These environmentally sensitive bottles help eliminate waste and help to ensure product integrity for long term storage. Clear / Flint Boston Round Bottles offers maximum visibility and sample integrity. Amber & Cobalt Blue Boston Round Bottles protect contents from UV rays and are ideal for light sensitive products. Composite Test Jars Clear / Flint Composite Test Jars are clear wide-mouth straight sided jars that are ideal for sampling and provide maximum content visibility. These bottles are designed without shoulders for maximum storage capacity. French Square Bottles Clear / Flint French Square Bottles provide maximum content visibility. The space saving design saves on shelf and storage space. The wide mouth opening is ideal for mixing, storing and sampling. Graduated Medium Round Bottles Bottle Beakers® also known as Graduated Medium Rounds are excellent for use with biological and pathological specimens, but can also be used for storing industrial laboratory chemicals and reagents. These clear / flint bottles are designed with a slight shoulder for easy pouring and handling. Graduated in ml and ounces. Mix, measure, and store in the same container. Media Bottles Media Bottles are manufactured from PYREX® borosilicate glass for chemical and thermal resistance and can be used for storage as well as mixing and sampling. Regular Media Bottles have permanent white enamel graduations and marking spots. PYREXPlus® Media Bottles have a protective PVC coating helps prevent glass from shattering and reduces spills.