Mechanisms of Recombination: 50 th Anniversary Meeting of the Holliday Model
May 19-23, 2014 Alicante, Spain
Monday, May 19
Afternoon Arrival
17:00 Registration
18:30 Welcome drinks
19:30 Keynote lectures:
Scott Keeney (Memorial Sloan Kettering Cancer Center, US) Mechanism and regulation of meiotic recombination initiation
David Sherratt (University of Oxford, UK) Recombination in live bacteria
Dinner
Tuesday, May 20
Breakfast
Session 1 Chairperson : Lorraine Symington
09:00 – 09:25 Roland Kanaar (Erasmus MC, The Netherlands) Molecular mechanism of homologous recombination
09:25 – 09:50 Patrick Sung (Yale University, US) Regulation of homologous recombination by DNA helicases
09:50 – 10:15 Steve Kowalczykowski (University of California, Davis, US) Seeing recombination: one molecule and step at a time
10:15 – 10:30 Xiaodong Zhang (Imperial College London, UK) Structural characterisations of BRCA2 provide mechanistic insights into Rad51 binding and filament formation during homologous recombination
Break
11:00 – 11:25 Akira Shinohara (Osaka University, Japan) Assembly and disassembly of Rad51 complex by Rad51 mediators and DNA helicases
11:25 – 11:50 Hiroshi Iwasaki (Tokyo Institute of Technology, Japan) The activation of Rad51-dirven DNA strand exchange reaction by the Swi5-Sfr1 complex
11:50 – 12:15 Wolf-Dietrich Heyer (University of California, Davis, US) Mechanism and regulation of recombinational DNA repair
12:15 – 12:40 Doug Bishop (University of Chicago, US) Recombinosome architecture and homolog bias in meiotic recombination
12:40 – 13:00 Lumír Krej čí (Masaryk University, Czech Republic) Interplay between sumoylation and recombination
Lunch
15:00 – 16:30 Poster session
Session 2 Chairperson : Stephen West
16:30 – 16:50 Robin Holliday (Australia)
16:50 – 17:15 John Rouse (University of Dundee, UK) Regulation of DNA repair nucleases in mammals
17:15 – 17:35 Boris Pfander (Max-Planck Institute of Biochemistry, Germany) A cell cycle-regulated Slx4-Dpb11 signalling complex controls the resolution of DNA repair intermediates linked to stalled replication
17:35 – 17:55 Pierre-Henri Gaillard (Marseilles Cancer Research Center, France) tba
17:55 – 18:15 Miguel Blanco (London Research Institute, UK) Control of Yen1 activity and localization by Cdk and Cdc14 prevents genome instability
18:15 – 18:35 Petr Cejka (University of Zurich, Switzerland The Mlh1-Mlh3 heterodimer is an endonuclease that preferentially binds to Holliday junctions
Break
19:05 – 19:25 Jeff Sekelsky (University of North Carolina, US) Functional, biochemical, and structural properties of Drosophila Holliday junction resolvases
19:25 – 19:45 Dana Branzei (IFOM-IEO Campus, Italy) Pathways involved in the resolution of mitotic DNA damage tolerance intermediates
19:45 – 20:10 Neil Hunter (University of California, Davis, US) Joint molecule metabolism during meiosis
20:10 – 20:35 Michael Lichten (National Cancer Institute, US) tba
Dinner
Wednesday, May 21
Breakfast
Session 3 Chairperson : Scott Keeney
09:00 – 09:25 Ken Marians (Sloan-Kettering Institute, US) tba
09:25 – 09:50 Lorraine Symington (Columbia University, US) DNA end resection and repair pathway choice
09:50 – 10:15 Rodney Rothstein (Columbia University, US) Increased chromosome mobility facilitates homology search during double-strand break repair
10:15 – 10:30 David Leach (University of Edinburgh, UK) DNA double-strand break repair in E. coli
Break
11:00 – 11:25 Stefan Jentsch (Max Planck Institute, Germany) Mechanisms of homology search
11:25 – 11:50 Susan Gasser (Friedrich Miescher Institute, Switzerland) Chromatin dynamics and the homology search
11:50 – 12:15 Monica Colaiacovo (Harvard Medical School, US) Germline maintenance and mechanisms of meiotic recombination in C. elegans
12:15 – 12:40 Galina Petukhova (Uniformed Services University of Health Studies, US) Initiation of genetic recombination in mammals
12:40 – 13:00 Angelos Constantinou (Institute of Human Genetics, CNRS, France) Replisome surveillance by FANCD2
13:00 – 13:15 Xiaolan Zhao (Memorial Sloan-Kettering Cancer Center, US) Smc5/6- and SUMO-based regulation of recombination intermediate metabolism
Lunch
Session 4 Chairperson : Rodney Rothstein
17:00 – 17:25 Andrés Aguilera (CABIMER, Spain) Links between R-loops and chromatin modifications in recombination-mediated genome instability
17:25 – 17:50 James Haber (Brandeis University, US) Repair of endonuclease-induced DSBs
17:50 – 18:15 Titia de Lange (The Rockefeller University, US) Telomeres and the DDR
18:15 – 18:35 Michael Cox (University of Wisconsin - Madison, US) Towards facile genome editing with the RecA-dependent nuclease Ref
Break
19:05 – 19:30 Alain Nicolas (Institut Curie, France) Biological role of G-quadruplexes in replication and genome instability
19:30 – 19:55 Bernard de Massy (Institute of Human Genetics, CNRS, France) The programmed induction of DNA double strand breaks during meiosis: molecular mechanism and implications
19:55 – 20:20 Maria Jasin (Sloan-Kettering Institute, US) tba
20:20 – 20:35 Sharon Cantor (University of Massachusetts Medical School, US) A novel mechanism of therapy resistance in BRCA2-mutant cell
Dinner
Thursday, May 22
Breakfast
Session 5 Chairperson : Roland Kanaar
09:00 – 09:25 Agata Smogorzewska (The Rockefeller University, US) Fanconi anemia and Fanconi anemia-like disorders
09:25 – 09:50 Ian Hickson (Copenhagen University, Denmark) Functional consequences of defective Holliday junction dissolution
09:50 – 10:15 Jos Jonkers (Netherlands Cancer Institute, The Netherlands) tba
10:15 – 10:35 Simon Powell (Memorial Sloan Kettering Cancer Center, US) Site-specific DNA replication block in human cells elicits fork cleavage by SLX4 and Mus81
Break
11:05 – 11:30 Simon Boulton (London Research Institute, UK) Genome stability and the control of homologous recombination
11:30 – 11:55 Penny Jeggo (University of Sussex, UK) From non-homologous end-joining to homologous recombination; a regulated switch in repair mechanism usage
11:55 – 12:20 Dale Wigley (Institute of Cancer Research, UK) The interaction of AddAB helicase/nuclease with the recombination hotspot, Chi
12:20 – 12:45 John Tainer (Scripps Resarch Institute, US) MRE11-RAD50 conformations and pathway choice at dsDNA breaks
12:45 – 13:05 Julian Sale (MRC Laboratory of Molecular Biology, UK) Epigenetic instability induced by structured DNA and replication stress
Lunch
15:30 – 17:00 Poster Session
Session 6 Chairperson : Agata Smogorzewska
17:00 – 17:25 Virginia Zakian (Princeton University, US) Pif1 family helicases: multiple roles in preserving genome integrity
17:25 – 17:50 Marco Foiani (IFOM - F.I.R.C. Inst. of Molecular Oncology Foundation, Italy) ATR-mediated control of chromosome integrity
17:50 – 18:15 Steve Jackson (University of Cambridge, UK) Assembly and disassembly of protein complexes at sites of DNA double-strand breaks
18:15 – 18:30 Alessandro Sartori (University of Zurich, Switzerland) FANCD2 recruits CtIP to promote DNA-end resection during the repair of DNA interstrand crosslinks
Break
19:00 – 19:25 Luis Aragón (Imperial College London, UK) Smc5/6-dependent regulation of recombination intermediates at damaged replication forks
19:25 – 19:50 Massimo Lopes (University of Zurich, Switzerland) Remodelling of replication intermediates upon replication stress
19:50 – 20:15 John Petrini (Sloan-Kettering Institute, US) Genetic analysis of chromosome break metabolism in eukaryotic cells
Gala Dinner
Friday, May 23
Breakfast
Morning Departure
* Please note, these timings are provisional and are subject to change.