Mechanisms of Recombination: 50 th Anniversary Meeting of the Holliday Model May 19-23, 2014 Alicante, Spain Monday, May 19 Afternoon Arrival 17:00 Registration 18:30 Welcome drinks 19:30 Keynote lectures: Scott Keeney (Memorial Sloan Kettering Cancer Center, US) Mechanism and regulation of meiotic recombination initiation David Sherratt (University of Oxford, UK) Recombination in live bacteria Dinner Tuesday, May 20 Breakfast Session 1 Chairperson : Lorraine Symington 09:00 – 09:25 Roland Kanaar (Erasmus MC, The Netherlands) Molecular mechanism of homologous recombination 09:25 – 09:50 Patrick Sung (Yale University, US) Regulation of homologous recombination by DNA helicases 09:50 – 10:15 Steve Kowalczykowski (University of California, Davis, US) Seeing recombination: one molecule and step at a time 10:15 – 10:30 Xiaodong Zhang (Imperial College London, UK) Structural characterisations of BRCA2 provide mechanistic insights into Rad51 binding and filament formation during homologous recombination Break 11:00 – 11:25 Akira Shinohara (Osaka University, Japan) Assembly and disassembly of Rad51 complex by Rad51 mediators and DNA helicases 11:25 – 11:50 Hiroshi Iwasaki (Tokyo Institute of Technology, Japan) The activation of Rad51-dirven DNA strand exchange reaction by the Swi5-Sfr1 complex 11:50 – 12:15 Wolf-Dietrich Heyer (University of California, Davis, US) Mechanism and regulation of recombinational DNA repair 12:15 – 12:40 Doug Bishop (University of Chicago, US) Recombinosome architecture and homolog bias in meiotic recombination 12:40 – 13:00 Lumír Krej čí (Masaryk University, Czech Republic) Interplay between sumoylation and recombination Lunch 15:00 – 16:30 Poster session Session 2 Chairperson : Stephen West 16:30 – 16:50 Robin Holliday (Australia) 16:50 – 17:15 John Rouse (University of Dundee, UK) Regulation of DNA repair nucleases in mammals 17:15 – 17:35 Boris Pfander (Max-Planck Institute of Biochemistry, Germany) A cell cycle-regulated Slx4-Dpb11 signalling complex controls the resolution of DNA repair intermediates linked to stalled replication 17:35 – 17:55 Pierre-Henri Gaillard (Marseilles Cancer Research Center, France) tba 17:55 – 18:15 Miguel Blanco (London Research Institute, UK) Control of Yen1 activity and localization by Cdk and Cdc14 prevents genome instability 18:15 – 18:35 Petr Cejka (University of Zurich, Switzerland The Mlh1-Mlh3 heterodimer is an endonuclease that preferentially binds to Holliday junctions Break 19:05 – 19:25 Jeff Sekelsky (University of North Carolina, US) Functional, biochemical, and structural properties of Drosophila Holliday junction resolvases 19:25 – 19:45 Dana Branzei (IFOM-IEO Campus, Italy) Pathways involved in the resolution of mitotic DNA damage tolerance intermediates 19:45 – 20:10 Neil Hunter (University of California, Davis, US) Joint molecule metabolism during meiosis 20:10 – 20:35 Michael Lichten (National Cancer Institute, US) tba Dinner Wednesday, May 21 Breakfast Session 3 Chairperson : Scott Keeney 09:00 – 09:25 Ken Marians (Sloan-Kettering Institute, US) tba 09:25 – 09:50 Lorraine Symington (Columbia University, US) DNA end resection and repair pathway choice 09:50 – 10:15 Rodney Rothstein (Columbia University, US) Increased chromosome mobility facilitates homology search during double-strand break repair 10:15 – 10:30 David Leach (University of Edinburgh, UK) DNA double-strand break repair in E. coli Break 11:00 – 11:25 Stefan Jentsch (Max Planck Institute, Germany) Mechanisms of homology search 11:25 – 11:50 Susan Gasser (Friedrich Miescher Institute, Switzerland) Chromatin dynamics and the homology search 11:50 – 12:15 Monica Colaiacovo (Harvard Medical School, US) Germline maintenance and mechanisms of meiotic recombination in C. elegans 12:15 – 12:40 Galina Petukhova (Uniformed Services University of Health Studies, US) Initiation of genetic recombination in mammals 12:40 – 13:00 Angelos Constantinou (Institute of Human Genetics, CNRS, France) Replisome surveillance by FANCD2 13:00 – 13:15 Xiaolan Zhao (Memorial Sloan-Kettering Cancer Center, US) Smc5/6- and SUMO-based regulation of recombination intermediate metabolism Lunch Session 4 Chairperson : Rodney Rothstein 17:00 – 17:25 Andrés Aguilera (CABIMER, Spain) Links between R-loops and chromatin modifications in recombination-mediated genome instability 17:25 – 17:50 James Haber (Brandeis University, US) Repair of endonuclease-induced DSBs 17:50 – 18:15 Titia de Lange (The Rockefeller University, US) Telomeres and the DDR 18:15 – 18:35 Michael Cox (University of Wisconsin - Madison, US) Towards facile genome editing with the RecA-dependent nuclease Ref Break 19:05 – 19:30 Alain Nicolas (Institut Curie, France) Biological role of G-quadruplexes in replication and genome instability 19:30 – 19:55 Bernard de Massy (Institute of Human Genetics, CNRS, France) The programmed induction of DNA double strand breaks during meiosis: molecular mechanism and implications 19:55 – 20:20 Maria Jasin (Sloan-Kettering Institute, US) tba 20:20 – 20:35 Sharon Cantor (University of Massachusetts Medical School, US) A novel mechanism of therapy resistance in BRCA2-mutant cell Dinner Thursday, May 22 Breakfast Session 5 Chairperson : Roland Kanaar 09:00 – 09:25 Agata Smogorzewska (The Rockefeller University, US) Fanconi anemia and Fanconi anemia-like disorders 09:25 – 09:50 Ian Hickson (Copenhagen University, Denmark) Functional consequences of defective Holliday junction dissolution 09:50 – 10:15 Jos Jonkers (Netherlands Cancer Institute, The Netherlands) tba 10:15 – 10:35 Simon Powell (Memorial Sloan Kettering Cancer Center, US) Site-specific DNA replication block in human cells elicits fork cleavage by SLX4 and Mus81 Break 11:05 – 11:30 Simon Boulton (London Research Institute, UK) Genome stability and the control of homologous recombination 11:30 – 11:55 Penny Jeggo (University of Sussex, UK) From non-homologous end-joining to homologous recombination; a regulated switch in repair mechanism usage 11:55 – 12:20 Dale Wigley (Institute of Cancer Research, UK) The interaction of AddAB helicase/nuclease with the recombination hotspot, Chi 12:20 – 12:45 John Tainer (Scripps Resarch Institute, US) MRE11-RAD50 conformations and pathway choice at dsDNA breaks 12:45 – 13:05 Julian Sale (MRC Laboratory of Molecular Biology, UK) Epigenetic instability induced by structured DNA and replication stress Lunch 15:30 – 17:00 Poster Session Session 6 Chairperson : Agata Smogorzewska 17:00 – 17:25 Virginia Zakian (Princeton University, US) Pif1 family helicases: multiple roles in preserving genome integrity 17:25 – 17:50 Marco Foiani (IFOM - F.I.R.C. Inst. of Molecular Oncology Foundation, Italy) ATR-mediated control of chromosome integrity 17:50 – 18:15 Steve Jackson (University of Cambridge, UK) Assembly and disassembly of protein complexes at sites of DNA double-strand breaks 18:15 – 18:30 Alessandro Sartori (University of Zurich, Switzerland) FANCD2 recruits CtIP to promote DNA-end resection during the repair of DNA interstrand crosslinks Break 19:00 – 19:25 Luis Aragón (Imperial College London, UK) Smc5/6-dependent regulation of recombination intermediates at damaged replication forks 19:25 – 19:50 Massimo Lopes (University of Zurich, Switzerland) Remodelling of replication intermediates upon replication stress 19:50 – 20:15 John Petrini (Sloan-Kettering Institute, US) Genetic analysis of chromosome break metabolism in eukaryotic cells Gala Dinner Friday, May 23 Breakfast Morning Departure * Please note, these timings are provisional and are subject to change. .