2012-2013 Seminars
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Suppression of Spontaneous Genome Rearrangements in Yeast DNA Helicase Mutants
Suppression of spontaneous genome rearrangements in yeast DNA helicase mutants Kristina H. Schmidt*†‡ and Richard D. Kolodner*§¶ *Ludwig Institute for Cancer Research and §Departments of Medicine and Cellular and Molecular Medicine and Cancer Center, University of California at San Diego, La Jolla, CA 92093; and †Division of Cell Biology, Microbiology, and Molecular Biology, Department of Biology, University of South Florida, Tampa, FL 33620 Contributed by Richard D. Kolodner, October 2, 2006 (sent for review June 16, 2006) Saccharomyces cerevisiae mutants lacking two of the three DNA the hyperrecombination and DNA-damage sensitive phenotypes of helicases Sgs1, Srs2, and Rrm3 exhibit slow growth that is sup- srs2 mutants are suppressed by HR defects (31). The physical pressed by disrupting homologous recombination. Cells lacking interaction between Srs2 and Pol32, a structural subunit of DNA Sgs1 and Rrm3 accumulate gross-chromosomal rearrangements polymerase delta, suggests that Srs2 may act during DNA replica- (GCRs) that are suppressed by the DNA damage checkpoint and by tion (32). Srs2 also is required for proper activation of Rad53 in homologous recombination-defective mutations. In contrast, rrm3, response to DNA-damaging agents (7, 33), and Srs2 itself is srs2, and srs2 rrm3 mutants have wild-type GCR rates. GCR types in phosphorylated after cells are exposed to methyl-methanesulfon- helicase double mutants include telomere additions, transloca- ate, hydroxyurea, or UV light; however, the significance of this tions, and broken DNAs healed by a complex process of hairpin- phosphorylation is unknown (33). mediated inversion. Spontaneous activation of the Rad53 check- Unlike Sgs1 and Srs2, the Rrm3 helicase has 5Ј-to-3Ј polarity and point kinase in the rrm3 mutant depends on the Mec3͞Rad24 DNA shares homology throughout its helicase domain with the S. -
2008 MD/Phd Program
Robert Wood Johnson Medical School MD/PhD Program Symposium Thursday, July 31, 2008 Department of Molecular Biology Carl Icahn Laboratory Princeton University Princeton, New Jersey Program Continental Breakfast 8:30 to 9:00 a.m. Introductory Remarks 9:00 to 9:30 a.m. Terri Goss Kinzy, PhD Assistant Dean for Medical Scientist Training Director, UMDNJ-RWJMS/Rutgers/Princeton MD/PhD Program Professor, Department of Molecular Genetics, Microbiology, and Immunology, UMDNJ-RWJMS James Broach, PhD Associate Director, Lewis-Sigler Institute for Integrative Genomics, Princeton University Professor and Associate Chair, Department of Molecular Biology, Princeton University Student Presentations (Session 1) 9:30 to 10:30 a.m. Break 10:30 to 10:45 a.m. Student Presentations (Session 2) 10:45 to 11:45 a.m. Break 11:45 to 12:00 p.m. Dean’s Welcome Remarks 12:00 to 12:15 p.m. Peter Amenta, MD, PhD Interim Dean, UMDNJ-RWJMS Professor and Chair, Department of Pathology and Laboratory Medicine, UMDNJ-RWJMS Luncheon 12:15 to 2:00 p.m. Student Presentations (Session 3) 2:00 to 3:00 p.m. Break 3:00 to 3:15 p.m. Keynote Address 3:15 to 4:15 p.m. Leon Rosenberg, MD Professor, Department of Molecular Biology, Princeton University and Woodrow Wilson School of Public and International Affairs “Questions a Physician-Scientist Asks About Life” Concluding Remarks 4:15 to 4:30 p.m. Elizabeth Gavis, MD, PhD Princeton Liason, UMDNJ-RWJMS/Rutgers/Princeton MD/PhD Program Professor, Department of Molecular Biology, Princeton University Reception 4:30 p.m. Acknowledgments: The MD/PhD Symposium was made possible by the support of Dr. -
Mechanisms of Recombination: 50 Th Anniversary Meeting of the Holliday Model
Mechanisms of Recombination: 50 th Anniversary Meeting of the Holliday Model May 19-23, 2014 Alicante, Spain Monday, May 19 Afternoon Arrival 17:00 Registration 18:30 Welcome drinks 19:30 Keynote lectures: Scott Keeney (Memorial Sloan Kettering Cancer Center, US) Mechanism and regulation of meiotic recombination initiation David Sherratt (University of Oxford, UK) Recombination in live bacteria Dinner Tuesday, May 20 Breakfast Session 1 Chairperson : Lorraine Symington 09:00 – 09:25 Roland Kanaar (Erasmus MC, The Netherlands) Molecular mechanism of homologous recombination 09:25 – 09:50 Patrick Sung (Yale University, US) Regulation of homologous recombination by DNA helicases 09:50 – 10:15 Steve Kowalczykowski (University of California, Davis, US) Seeing recombination: one molecule and step at a time 10:15 – 10:30 Xiaodong Zhang (Imperial College London, UK) Structural characterisations of BRCA2 provide mechanistic insights into Rad51 binding and filament formation during homologous recombination Break 11:00 – 11:25 Akira Shinohara (Osaka University, Japan) Assembly and disassembly of Rad51 complex by Rad51 mediators and DNA helicases 11:25 – 11:50 Hiroshi Iwasaki (Tokyo Institute of Technology, Japan) The activation of Rad51-dirven DNA strand exchange reaction by the Swi5-Sfr1 complex 11:50 – 12:15 Wolf-Dietrich Heyer (University of California, Davis, US) Mechanism and regulation of recombinational DNA repair 12:15 – 12:40 Doug Bishop (University of Chicago, US) Recombinosome architecture and homolog bias in meiotic recombination 12:40 -
Virginia Zakian CV
VIRGINIA ARAXIE ZAKIAN Curriculum Vitae PRESENT POSITION AND ADDRESS Harry C. Wiess Professor in the Life Sciences Department of Molecular Biology Princeton University Princeton NJ 08544-1014 Phone: (609) 258-6770 ; FAX: (609) 258-1701 ; email: [email protected] CITIZENSHIP: U.S.A. RESEARCH INTERESTS Telomeres, DNA helicases, Replication fork progression, Chromosome stability, Genome integrity EDUCATION, RESEARCH EXPERIENCE, AND PROFESSIONAL POSITIONS A.B. 1970, Cornell University, College of Arts and Sciences, Ithaca, NY (Phi Beta Kappa, 1969; cum laude in Biology; distinction in all subjects; research Xenopus development, with Dr. A.W. Blackler). Ph.D. 1975, Yale University, Dept. of Biology (with Dr. Joseph G. Gall, DNA replication in Drosophila; NDF pre-doctoral fellowship). Postdoctoral Fellow 1975-76, Princeton University, Dept. of Biochemistry (with Dr. Arnold J. Levine, Replication of Adeno and SV40 viruses; NIH post doctoral fellowship) Postdoctoral Fellow 1976-78, University of Washington, Dept. of Genetics (with Dr. Walton L. Fangman, DNA replication in yeast; NIH post doctoral fellowship) Assistant Member 1979-83, Fred Hutchinson Cancer Research Center, Basic Sciences Associate Member 1984-1987, Fred Hutchinson Cancer Research Center, Basic Sciences Member 1987-1995, Fred Hutchinson Cancer Research Center, Basic Sciences (tenured position) Affiliate Faculty 1979-1995, U. of Washington (Depts. of Genetics and Pathology) Professor, Department of Molecular Biology, Princeton University, July 1995- to date Harry C. Wiess Professor -
The Yeast Pif1 Helicase Prevents Genomic Instability Caused by G-Quadruplex-Forming CEB1 Sequences in Vivo
The Yeast Pif1 Helicase Prevents Genomic Instability Caused by G-Quadruplex-Forming CEB1 Sequences In Vivo Cyril Ribeyre, Judith Lopes, Jean-Baptiste Boulé, Aurele Piazza, Aurore Guédin, Virginia Zakian, Jean-Louis Mergny, Alain Nicolas To cite this version: Cyril Ribeyre, Judith Lopes, Jean-Baptiste Boulé, Aurele Piazza, Aurore Guédin, et al.. The Yeast Pif1 Helicase Prevents Genomic Instability Caused by G-Quadruplex-Forming CEB1 Sequences In Vivo. PLoS Genetics, Public Library of Science, 2009, 5 (5), pp.e1000475. 10.1371/jour- nal.pgen.1000475. hal-02109281 HAL Id: hal-02109281 https://hal.archives-ouvertes.fr/hal-02109281 Submitted on 24 Apr 2019 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. The Yeast Pif1 Helicase Prevents Genomic Instability Caused by G-Quadruplex-Forming CEB1 Sequences In Vivo Cyril Ribeyre1.¤, Judith Lopes1., Jean-Baptiste Boule´ 1,2, Aure` le Piazza1, Aurore Gue´din3, Virginia A. Zakian2, Jean-Louis Mergny3, Alain Nicolas1* 1 Recombinaison et Instabilite´ Ge´ne´tique, Institut Curie Centre de Recherche, CNRS UMR3244, Universite´ Pierre et Marie Curie, Paris, France, 2 Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America, 3 Laboratoire de Biophysique, Museum National d’Histoire Naturelle USM 503, INSERM U565, CNRS UMR5153, Paris, France Abstract In budding yeast, the Pif1 DNA helicase is involved in the maintenance of both nuclear and mitochondrial genomes, but its role in these processes is still poorly understood. -
Yeast Chromosome Biology & Cell Cycle
Yeast Chromosome Biology & Cell Cycle July 15-July 20, 2018 Steamboat Grand, Steamboat Springs, Colorado Organizers: Jennifer Gerton, Investigator Stowers Institute for Medical Research Kansas City, Missouri M. K. Raghuraman (Raghu), Research Professor University of Washington Seattle, Washington Sunday, July 15, 2018 Time Title 4:00 p.m. – 9:00 p.m. Conference Registration 6:00 p.m. – 7:00 p.m. Welcome Reception, open bar 7:00 p.m. – 8:30 p.m. DINNER 8:45 p.m. – 10:00 p.m. Keynote Address David Morgan, University of California San Francisco Mechanisms underlying the initiation of chromosome segregation Monday, July 16, 2018 Time Title 7:30 a.m. – 9:00 a.m. BREAKFAST 7:30 a.m. – 12:00 p.m. Conference Registration 9:00 a.m. – 12:15 p.m. GENERAL SESSION 1: Chromosome Structure Chair: Frank Uhlmann, The Francis Crick Institute 9:10 a.m. – 9:35 a.m. Doug Koshland, University of California Berkeley Cohesin, a complex mechanism for DNA binding 9:35 a.m. – 10:00 a.m. Christian Haering, European Molecular Biology Laboratory Condensin complexes structure chromosomes by active loop extrusion 10:00 a.m. – 10:20 a.m. Damien D'Amours, University of Ottawa Condensin ATPase motifs contribute differentially to the maintenance of chromosome morphology and genome stability 10:20 a.m. – 10:50 a.m. MORNING COFFEE BREAK & GROUP PHOTO 10:50 a.m. – 11:15 a.m. Kerry Bloom, University of North Carolina at Chapel Hill Fork pausing allows centromere DNA loop formation and kinetochore assembly 11:15 a.m. – 11:40 p.m. -
April 5, 2018
STUPKA UNDERGRADUATE RESEARCH SYMPOSIUM April 5, 2018 Presented by the Undergraduates of the Roy J. Carver Department of Biochemistry, Biophysics & Molecular Biology EVENTS SCHEDULE THURSDAY 8:30 am Breakfast – Open to all – Hosted by BBMB Breakfast Club Keynote and Alumni Speaker Meet and Greet Atrium 11:00 am Alumni and Stupka Family Reception and Lunch Atrium 12:10 pm Dr. Virginia Zakian Lunch Undergraduates and Graduates Only 1102 MBB 1:10 pm Dr. Douglas Weibel Lunch Undergraduates and Graduates Only 1102 MBB 2:10 pm Poster Session 1 Atrium 3:10 pm Poster Session 2 Atrium 4:10 pm Welcome Wendy Wintersteen, President of Iowa State University Beate Schmittmann, Dean of the College of Liberal Arts and Sciences 1414 MBB 4:20 pm Jena Gilbertson – STUDENT SPEAKER Imaging the Non-uniform Spatial Distribution Energy Dense Metabolites for Efficient Capture and Chemical Storage of Solar Energy by Plants 4:35 pm Dr. Virginia Zakian – KEYNOTE SPEAKER Stressing at the ends: telomerase regulation 5:15 pm Matthew Cook – STUDENT SPEAKER Optimizing the biosynthetic pathway producing UDP-xylose 5:30 pm Break and Refreshments 5:50 pm Andrew Tonsager – STUDENT SPEAKER Investigating the Arabidopsis QQS gene and its impact on carbon-nitrogen partitioning in Saccharomyces cerevisiae 6:05 pm Anthony Cyr, M.D, Ph.D – ALUMNI SPEAKER Breaking the Cycle: Mitochondrial responses to traumatic injury in cells of the innate immune system 6:30 pm Dr. Douglas Weibel – KEYNOTE SPEAKER Mechanical Genomics Reveals New Bacterial Biochemistry 7:10 pm Poster Awards 1414 MBB 7:15 pm Dinner Atrium 2 STUPKA 2018 TABLE OF CONTENTS REMEMBERING ROB 4 STUPKA COMMITTEE 5 POSTER SESSIONS 6 – 7 SPEAKERS 8 – 13 STUPKA SCHOLARS 14 – 15 SPONSORS 16 6 0 0 2 E C N I S SAVE THE DATE FOR THE 14TH ANNUAL Stupka Symposium Thursday, April 4, 2019 email: [email protected] website: stupka.bb.iastate.edu UNDERGRADUATE RESEARCH SYMPOSIUM 3 REMEMBERING ROB Rob Stupka was an undergraduate student majoring in Biochemistry at Iowa State University. -
VIRGINIA ARAXIE ZAKIAN Curriculum Vitae PRESENT POSITION and ADDRESS Harry C
VIRGINIA ARAXIE ZAKIAN Curriculum Vitae PRESENT POSITION AND ADDRESS Harry C. Wiess Professor in the Life Sciences Department of Molecular Biology Princeton University Princeton NJ 08544-1014 Phone: (609) 258-6770 ; FAX: (609) 258-1701 ; email: [email protected] CITIZENSHIP: U.S.A. RESEARCH INTERESTS Telomeres, DNA helicases, Chromosome stability, Genome integrity EDUCATION, RESEARCH EXPERIENCE, AND PROFESSIONAL POSITIONS A.B. 1970, Cornell University, College of Arts and Sciences, Ithaca, NY (Phi Beta Kappa, 1969; cum laude in Biology; distinction in all subjects; research Xenopus development, with Dr. A.W. Blackler). Ph.D. 1975, Yale University, Dept. of Biology (with Dr. Joseph G. Gall, DNA replication in Drosophila; NDF pre-doctoral fellowship). Postdoctoral Fellow 1975-76, Princeton University, Dept. of Biochemistry (with Dr. Arnold J. Levine, Replication of Adeno and SV40 viruses; NIH post doctoral fellowship) Postdoctoral Fellow 1976-78, University of Washington, Dept. of Genetics (with Dr. Walton L. Fangman, DNA replication in yeast; NIH post doctoral fellowship) Assistant Member 1979-83, Fred Hutchinson Cancer Research Center, Basic Sciences Associate Member 1984-1987, Fred Hutchinson Cancer Research Center, Basic Sciences Member 1987-1995, Fred Hutchinson Cancer Research Center, Basic Sciences (tenured position) Affiliate Faculty 1979-1995, U. of Washington (Depts. of Genetics and Pathology) Professor, Department of Molecular Biology, Princeton University, July 1995- to date Harry C. Wiess Professor in the Life Sciences, -
Dr. Jorge Torres, Associate Professor 2016-2019, Associate Professor Dept
Dr. Jorge Torres, Associate Professor 2016-2019, Associate Professor Dept. of Chem and Biochem, University of California, Los Angeles 2009-2016, Assistant Professor Dept. of Chem and Biochem, University of California, Los Angeles EDUCATION 2006-2009 Postdoctoral Fellow, Genentech Inc., South San Francisco, CA Department of Tumor Biology and Angiogenesis 2004-2005 Postdoctoral Fellow, Stanford University, Palo Alto, CA Department of Pathology 1998-2004 Ph.D., Princeton University, Princeton, NJ Department of Molecular Biology 1994-1998 B.S., University of California, Santa Barbara, CA Department of Molecular Cellular and Developmental Biology HONORS & AWARDS 2019 Ruth Kirschstein Diversity in Science Award, American Society for Biochemistry and Molecular Biology (ASBMB) 2019 Student Development Diversity, Equity and Inclusion (DEI) Award, UCLA 2016 Glenn T. Seaborg Award, UCLA 2014 American Cancer Society Research Scholar Award 2013 Faculty Career Development Award, UCLA 2013 Cottrell Scholar Award, Research Corporation for Science Advancement 2012 Herbert Newby McCoy Award, UCLA 2012 Faculty Career Development Award, UCLA 2011 Basil O’Connor Award, March of Dimes Foundation 2010 V Scholar Award, The V Foundation for Cancer Research 2009 John McTague Career Development Chair, UCLA 2005 Leukemia and Lymphoma Society Postdoctoral Fellowship 2005 Ruth L. Kirschstein National Research Service Award, NIH (declined) 2004 Stanford University Cancer Biology Postdoctoral Fellowship 2003 Leadership Alliance/Schering-Plough Fellowship 1999 Princeton -
VIRGINIA ARAXIE ZAKIAN Curriculum Vitae PRESENT POSITION and ADDRESS Harry C
VIRGINIA ARAXIE ZAKIAN Curriculum Vitae PRESENT POSITION AND ADDRESS Harry C. Wiess Professor in the Life Sciences Department of Molecular Biology Princeton University Princeton NJ 08544-1014 Phone: (609) 258-6770 ; FAX: (609) 258-1701 ; email: [email protected] CITIZENSHIP: U.S.A. RESEARCH INTERESTS Telomeres, DNA helicases, Replication fork progression, Chromosome stability, Genome integrity EDUCATION, RESEARCH EXPERIENCE, AND PROFESSIONAL POSITIONS A.B. 1970, Cornell University, College of Arts and Sciences, Ithaca, NY (Phi Beta Kappa, 1969; cum laude in Biology; distinction in all subjects; research Xenopus development, with Dr. A.W. Blackler). Ph.D. 1975, Yale University, Dept. of Biology (with Dr. Joseph G. Gall, DNA replication in Drosophila; NDF pre-doctoral fellowship). Postdoctoral Fellow 1975-76, Princeton University, Dept. of Biochemistry (with Dr. Arnold J. Levine, Replication of Adeno and SV40 viruses; NIH post-doctoral fellowship) Postdoctoral Fellow 1976-78, University of Washington, Dept. of Genetics (with Dr. Walton L. Fangman, DNA replication in yeast; NIH post-doctoral fellowship) Assistant Member 1979-83, Fred Hutchinson Cancer Research Center, Basic Sciences Associate Member 1984-1987, Fred Hutchinson Cancer Research Center, Basic Sciences Member 1987-1995, Fred Hutchinson Cancer Research Center, Basic Sciences (tenured position) Affiliate Faculty 1979-1995, U. of Washington (Depts. of Genetics and Pathology) Professor, Department of Molecular Biology, Princeton University, July 1995- to date Harry C. Wiess Professor -
1996 Virginia Zakian Biology Caught Ginger Zakian's Imagination in the Ninth Grade and She Decided at That Time to Become A
1996 Virginia Zakian Biology caught Ginger Zakian’s imagination in the ninth grade and she decided at that time to become a scientist. After graduating from high school in a Philadelphia suburb as class valedictorian, Zakian attended Cornell University. She recalls that after 12 years of boredom, Cornell was "fantastic, I thought I had gone to heaven." During her freshman year, she was even able to do research in behavioral biology, studying the effects of crowding on guppy behavior. (One of the side benefits of this research was that it exempted her from the nightly curfew to which all female undergraduates were subject.) Her undergraduate research advisor was Tonie Blackler who was "the first teacher to challenge me academically." The first semester she worked in his lab, Blackler gave her a B+, not because she hadn't worked hard or well but because he felt she was 'intellectually lazy'. Presumably as planned, Blacker's lesson stayed with Zakian and motivated her to work harder. Zakian graduated cum laude in Biology in 1970. Following Cornell, Zakian moved to Yale because it had an excellent developmental biology program, a field that she thought she wanted to pursue. After a year of sampling various labs and not finding what she wanted, Joe Gall returned from sabbatical to Yale. The day he returned, Zakian and two other students were camped outside his door hoping to join his lab. Gall, unable to choose among them, took all three (the other two were Sharyn Endow and Patricia Pukkila). In Gall’s lab, Zakian worked on the replication of satellite DNA and was introduced to the wonders and complexity of eukaryotic chromosomes. -
Dr. Jorge Torres, Assistant Professor
CURRICULUM VITAE Dr. Jorge Torres, Assistant Professor 2009-Current Department of Chemistry and Biochemistry, University of California Los Angeles Office: 310-206-2092, Email: [email protected] EDUCATION Postdoctoral Fellow Genentech INC, South San Francisco, CA 2006-2009 Department of Tumor Biology and Angiogenesis Stanford University, Palo Alto, CA 2004-2005 Department of Pathology Ph.D. Princeton University, Princeton, NJ 2004 Department of Molecular Biology B.S. University of California, Santa Barbara, CA 1998 Department of Molecular Cellular and Developmental Biology HONORS & AWARDS American Cancer Society Research Scholar Award 2014 Faculty Career Development Award, UCLA 2013 Cottrell Scholar Award, Research Corporation for Science Advancement 2013 Herbert Newby McCoy Award 2012 Faculty Career Development Award, UCLA 2012 Basil OʼConnor Award, March of Dimes Foundation 2011 V Scholar Award, The V Foundation for Cancer Research 2010 John McTague Career Development Chair 2009 Leukemia and Lymphoma Society Postdoctoral Fellowship 2005 Ruth L. Kirschstein National Research Service Award, NIH (declined) 2005 Stanford University Cancer Biology Postdoctoral Fellowship 2004 Leadership Alliance/Schering-Plough Fellowship 2003 Princeton University Molecular Biology Teaching Award 1999 Ford Baccalaureate Incentive Award for Outstanding Undergraduate Research 1998 Graduation Commencement Speaker 1998 MEMBERSHIPS UCLA Biomedical Engineering Interdepartmental Program (BME-IDP) 2011-Current UCLA Chemistry-Biology Interface Training Program (CBI) 2010-Current American Society for Biochemistry and Molecular Biology (ASBMB) 2010-Current UCLA Jonsson Comprehensive Cancer Center (JCCC) 2009-Current UCLA Molecular Biology Institute (MBI) 2009-Current UCLA BMB and ACCESS Graduate Programs 2009-Current American Society for Cell Biology (ASCB) 2005-Current RESEARCH EXPERIENCE Postdoctoral Fellow Proteomic and functional characterization of the mitotic spindle 2006-2009 Advisor : Dr.