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For research purposes only, not for human use

Product Data Sheet

HIST1H4E siRNA (Human)

Catalog #

CRH5487

Source
Reactivity
Applications

Synthetic
H
RNAi

Description Specificity

siRNA to inhibit HIST1H4E expression using RNA interference HIST1H4E siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Form

Lyophilized powder

Gene Symbol Alternative Names Entrez Gene SwissProt

HIST1H4E H4/E; H4FE; Histone H4 8367 (Human) P62805 (Human)

Purity

> 97%

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency. We offers pre-designed sets of 3 different target-specific siRNA oligo duplexes of human HIST1H4E gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2’-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo. We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis.

Components Directions for Use

Application key: E- ELISA, WB- Western blot, IH- Immunohistochemistry, IF- Immunofluorescence, FC- Flow cytometry, IC- Immunocytochemistry, IP- Immunoprecipitation, ChIP- Chromatin Immunoprecipitation, EMSA- Electrophoretic Mobility Shift Assay, BL- Blocking, SE- Sandwich ELISA, CBE- Cell-based ELISA, RNAi- RNA interference Species reactivity key: H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, RbRabbit, S- Sheep, Z- Zebrafish

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For research purposes only, not for human use

Product Data Sheet

Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Storage/Stability

Shipped at 4 °C. Store at -20 °C for one year.

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