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PI4K2B Sirna Set I PI4K2B Sirna Set I

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PI4K2B Sirna Set I PI4K2B Sirna Set I Catalog # Aliquot Size P22-911B-05 3 x 5 nmol P22-911B-20 3 x 20 nmol P22-911B-50 3 x 50 nmol PI4K2B siRNA Set I siRNA duplexes targeted against three exon regions Catalog # P22-911B Lot # Z2073-40 Specificity Formulation PI4K2B siRNAs are designed to specifically knock-down The siRNAs are supplied as a lyophilized powder and human PI4K2B expression. shipped at room temperature. Product Description Reconstitution Protocol PI4K2B siRNA is a pool of three individual synthetic siRNA Briefly centrifuge the tubes (maximum RCF 4,000g) to duplexes designed to knock-down human PI4K2B mRNA collect lyophilized siRNA at the bottom of the tube. expression. Each siRNA is 19-25 bases in length. The gene Resuspend the siRNA in 50 µl of DEPC-treated water accession number is NM_018323. (supplied by researcher), which results in a 1x stock Gene Aliases solution (10 µM). Gently pipet the solution 3-5 times to mix FLJ11105; PI4KIIB; PIK42B and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage. Storage and Stability The lyophilized powder is stable for at least 4 weeks at Related Products room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or Product Name Catalog Number below -20oC. After resuspension, siRNA stock solutions ≥2 PI4K2A, Active P21-10G µM can undergo up to 50 freeze-thaw cycles without PI4K2B, Active P22-10G significant degradation. For long-term storage, it is PI4KB, Active P32-10G recommended that the siRNA is stored at -70oC. For most PI4KA Protein P31-30G favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Scientific Background PI4K2B is a member of the phosphatidylinositol 4-kinase family (PI4K) that phosphorylate phosphatidylinositol to generate phosphatidylinositol 4-phosphate which are PI4K2B siRNA Set I immediate precursor of several important signaling and siRNA duplexes targeted against three exon regions scaffolding molecules. PI4K2B is primarily a cytosolic PI4K Catalog # that is recruited to membranes where it stimulates P22-911B phosphatidylinositol 4,5-bisphosphate syntheses (1). Lot # Z2073-40 PI4K2B uses phosphatidylinositol as their primary substrate Packaging Specifications 2.5 nmol/tube for 3 x 5 nmol and has no activity on phosphatidylinositol Format Lyophilized powder o monophosphates. PI4K2B is a restricted minor Stability 1yr at -70 C from date of shipment Storage & Shipping The lyophilized powder is stable for at histocompatibility antigen in patients that had been least 4 weeks at room temperature. It is successfully treated with donor lymphocyte infusions for recommended that the lyophilized relapsed chronic myeloid leukemia after allogeneic stem and resuspended siRNAs are stored at or below -20oC. After resuspension, cell transplantation (2). siRNA stock solutions ≥2 µM can undergo up to 50 freeze-thaw cycles References without significant degradation. For 1. Balla, A. et.al: Characterization of type II long-term storage, it is recommended phosphatidylinositol 4-kinase isoforms reveals that the siRNA is stored at -70oC. For most favorable performance, avoid association of the enzymes with endosomal vesicular repeated handling and multiple compartments. J. Biol. Chem. 277: 20041-20050, freeze/thaw cycles. 2002. 2. Griffioen, M. et.al: Identification of phosphatidylinositol 4-kinase type II beta as HLA class II-restricted target in graft versus leukemia reactivity. Proc. Nat. Acad. Sci. 105: 3837-3842, 2008. To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS. .
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