ANNUAL REPORT 2011

PBRG | BIOTECHNOLOGY RESEARCH GROUP

PBRG Plant Biotechnology Research Group

Institute for Biotechnology and Bioengineering Departamento de Biologia Vegetal Faculdade de Ciências de Lisboa Campo Grande 1749-016 Lisbon Portugal http://cbv.fc.ul.pt PBRG topics have been under study during 2011: of bioactive components.following Briefly, the plant secretory structur on the terpene synthase characterization of aromatic species, focusing medicinal flora potentialities, the molecular sessment of the National endemic aromatic/ ondary metabolites production, aiming the as- from Algarve, c) Characterization of the volatiles tection in Propolis from beehives maintained at Volatiles characterizationb) and acaricide de- propolis volatiles (col. CIMO-IPB/FCUP), used for growing pineapples ( systems of micropropagated Pinus spp./ 1. Research Topics riannual basis, are the technology. The main research goals, on a plu- also involved in advanced education on bio- (PBRG) is a research unit on plant sciences The Plant Biotechnology Research Group Objectives (Transnational Cooperation Portugal-Morocco), Ormenis mixta sition and biological activity of the volatiles from and d) Characterization of the chemical compo- in São Miguel (Azores, Portugal) (Col. CBA), 2. a) Establishment of in vitro co-culture model a) Chemosystematic survey of Portuguese Plant Biotechnology Research Group leiva , a warm organic matter rich soil and in vivo es and the prospecting Paronychia argentea genes, the study of Ananas comosus and in vitro sec- )

storage period, fresh-cut fruit and their changes throughout the 6. Nutritional quality evaluation of fresh and dicinal species (col. CQB-FCUL, CBA-FCUL), explain some of the traditional uses of the me- and isolated phenolic constituents, that could ous plant extracts, infusions and decoctions, 5. Evaluation of the biological activities of aque- structures of medicinal chemistry and ultrastructure of the secretory the morphology,4. Study of anatomy, histo- ymorphism in this species, on the molecular elucidation of essential oil pol- micropropagated caespititius to assist invasion mechanisms, and b) establishment of todes) for characterization of the nematodes Meloidogyne chitwoodi (Potato-Cyst Nema- tode) and Solanum tuberosum hairy roots/ Bursaphlenchus xylophilus (Pine Wilt Nema- genes: TPS1 and TPS2, gous expression of prev types in this species, and b) Study of heterolo- lish its relationship with the different chemo- cDNA library of T. caespititius in order to estab- full-sequence gene, and b) construction of a design and RACE-PCR, leading to cloning the ble for other , starting with primer caespititius based on conserved regions availa- 3. a) Searching for TPS genes in Thymus and/or aromatic ,

iously identified TPS

Évora), xylophilus pinaster cellular changes induced in pine tissues ( todes, and c) evaluation of the histological and inoculated and inoculated plants with nema- healthy and 7 nus pinaster Chemical characterization of Portuguese nematode, the pine woodties, namely in the control of fractions, for their potential nematicidal proper- . a) Assessment of essential oils, and their and (collab. NemaLab – ICAM, Univ. P. pinea) and in lab in Bursaphlenchus xylophilus, P. pinea mechanically wounded un- by

a virulent strain of volatiles from both b) Pi- B. P.  Major Achievements bacterial gene expression. domonas myrcene used as sole carbon source by 8. Identification of the ously shown, suggests that with the radical scav activity. The inhibition of lysozyme along batus the bloodstream to different organs. and lysozyme, allowing them to be carried in bind to the human transport proteins albumin enin), found in the rat plasma, are able to olites (rosmarinic acid, luteolin and apig- barbatus intrinsic fluorescence analysis proved that tion of pure compounds. In addition, protein components is different from the administra- metabolization and bioavailability of the tea cholinesterase. It was also showed that the brain, where it inhibits the enzyme acetyl- blood braininthe detected barrier and be cross the intestinal barrier, as well the gastrically or intraperi intra- administered acid), (rosmarinic herbal tea and its main constituent nus, it was demonstrated that inflammatory raw material CQB, FCUL). (col. ter extract is a very promising anti- Pursuing our studies in the tea constituents also inhibit lysozyme sp. M1 and their correlation with extract components and its metab- catabolic products of enger capabilit toneally to rats, can toneally to rats, can Plectranthus P. barbatus P. barbatus y, previ- P. bar- Pseu-

ge- wa- P. β -

Plant Biotechnology Research Group PBRG  F. vulgare na from extracts chloroformic ized peroxide scavengers, respectively. Deodor- tracts were also effective as DPPH and su- activity. activity. hydrodistillation influenced the antioxidant radicals and it was verified that the time of and superoxideDPPH radicals anioning officinalis tioxidant and anti-inflammatory activities. matic species Different solvent-extractsfrom several aro- lipoxygenase activity inhibition. lipoxygenase activity , T. camphoratus O. vulgare extracts were the best in scaveng- and

were investigated for their an- S. officinalis , and T. carnosus T. mastichina exhibited high , T. mastichi- O. vulgare ex- S. ,       1.1 terase inhibition capacity with an IC50 = tial oil showed the strongest acetylcholines- out the storage period showed that citric acid The evaluation of quality changes through- (col. NemaLab – ICAM, Univ. Évora). Ruta graveolens experiments. Moreover, the essential oil of alternative solvent vehicle for this kind of that acetone seems to be an appropriate properties of essential oils, it was noticed In the assays for testing the nematicidal among individuals of A high genetic variab in the extracts. with the highest amounts of phenols present ent zones of Algarve, showed a coincidence dro-alcoholic extracts of propolis, from differ- The evaluation of antioxidant activity hy- used. showed no citotoxicity to the gastric cell line ed the intracellular growth of nent, carvacrol significantly ( Central (Pico, Azores) and its main compo- T. caespititius Moreover, tivity. ation in terms anti-acetylcholinesterase ac- Thymus The essential oils from several Portuguese gies to help its eradication and/or control. mercial uses as well management strate- species may assist finding its potential com- tailed characterizationweed of this alien species in the Azores archipelago. The de- that can explain the invasive ability of this μ g/ml. species, showed a quite broad vari- essential oil from Planalto showed a notorious activity T. zygis Pittosporum undulatum subsp. ility was observed p <0.05) inhibit- H. pylori zygis essen- , and

Dandlen S.A., A.S. Lima, M.D. Mendes, M.G. M.D. Mendes, Dandlen S.A., A.S. Lima, Dandlen S.A., M.G. Miguel, J.M. Duarte, M.L. Selected Publications     partial characterizationof cDNA and gDNA sequence information. The placement was confirmed by comparing tively. Gene structure, intron and exon synthase and Tctps2 longing to different chemotypes. caespititius also achieved. gene codingfor 150. nal of Essential Oil Bearing Plants guese tylcholinesterase inhibitionPortu- activity of Figueiredo, L.G. Pedro, J.G. Barroso. Ace- Faleiro, M.J. Sousa, A.S. Lima, A.C. U. Minho). sion of Preliminary study of heterologous expres- Tctps1 Study of tive TPS genes in Identification and characterization2 puta- of kiwifruit. taining the nutritional properties of fresh-cut and 2% ascorbic acid dip was better for re- treatment was better for preserving firmness, chemotypes, established in the field. on characterized , putatively encoding for sabinene and Thymus Tctps2 Tctps1

micropropagated cultures be- Tctps2 was performed (col. CEB/IBB, γ -terpinenerespec- synthase, and species essential oils.

1,8-cineole synthase, was T. caespititius T. were also identified and and identified also were Tctps2 T. caespititius, Tctps3 expression on

, of different , a putative , 14: 140-

Tctps1 Jour- T. , Miguel G., C. Cruz, M.L. Faleiro, M.T.F. Mendes M.D., A.S. Lima, H. Trindade, A.I.D. P.J.A. Madeira, M.H. Florêncio, L.Falé P.L.V., –541. activities. composition, antioxidant and antimicrobial fect of hydrodistillation time on the chemical Pedro. Simões, A.C. Figueiredo, Products archipelago (Portugal). undulatum and leaf volatiles analysis of Figueiredo. ISSR molecular characterization Correia, J.G. Barroso, L.G. Pedro, A.C. Function ronal acetylcholinesterase inhibitor. Plectranthus barbatus Ascensão, M.L.M. Serralheiro. Function of nosy sential oils. growth inhibition of Portuguese bial activity, cytotoxicity and intracellular Antimicro- Figueiredo. A.C. Barroso, J.G. ro, Faleiro, M. Miguel, M.L. , 21: 1012-1024. Salvia officinalis , 2: 130-136. , 33: 710-719. Natural Product Research Vent. naturalized in the Azores Brazilian Journal of Pharmacog- herbal tea as neu- L. essential oils: ef- Industrial Crops andIndustrial Crops J. Sousa, L.G. Ped- J. J.G. Barroso, L.G. Thymus Pittosporum , 25: 526 Food Food es-

Biological Sciences Research Group Plant Biotechnology Research Group Publications commercial essential oils and their major com- Articles in International Peer-Reviewed Journals pounds. Molecules, 16: 7672-7690. Smail A., B. Lyoussi, M.G. Miguel, Antioxidant Ahmadi N., Negrão, S., Katsantonis, D., Frouin activity, cytotoxicity and intracellular growth inhi- activity of some Morrocan hydrosols. Journal of J., Ploux, J., Letourmy, P., Droc, G., Babo, P., bition of Portuguese Thymus essential oils. Bra- Medicinal Plants Research, 5: 6688-6696. Trindade, H., Bruschi, G.,·Greco, R., Oliveira, zilian Journal of Pharmacognosy, 21: 1012-1024. M.M., Piffanelli, P., Courtois, B., Targeted asso- Falé P.L.V., L. Ascensão, M.L.M. Serralheiro, ciation analysis identified japonica rice varieties P.I. Haris, Interaction between Plectranthus bar- achieving Na+/K+ homeostasis without the allelic batus herbal tea components and human serum Book chapters make-up of the salt tolerant indica variety Nona albumin and lysozyme: binding and activity stud- Barata A.M., F. Rocha, V. Lopes, E. Betten- Bokra. Theor Appl Genet, 123: 881–895. ies. Spectroscopy, 26:79-92. court, A.C. Figueiredo, Medicinal and Aromatic Albano S., M.G. Miguel, Biological activities of Falé P.L.V., P.J.A. Madeira, M.H. Florêncio, L. Plants – Portugal, In: Medicinal and Aromatic extracts of plants grown in Portugal. Industrial Ascensão, M.L.M. Serralheiro, Function of Plants of The World, [Eds. M. Ozturk, G.-F. B. Crops and Products, 33: 338-343. Plectranthus barbatus herbal tea as neuronal Ameenah], Encyclopedia of Life Support Sys- acetylcholinesterase inhibitor. Food Function, 2: Belhattab R., M. Boudjouref, J.G. Barroso, tems (EOLSS), Developed under the Auspices of 130-136. L.G. Pedro, A.C. Figueirido, Essential oil com- the UNESCO, Eolss Publishers, Oxford, UK, Miguel M.G., M.D. Antunes, Is propolis safe as position from Artemisia campestris grown in Al- Figueiredo A.C. and L.G. Pedro, Book Review: [http://www.eolss.net]. an alternative medicine? Journal of Pharmacy geria. Advances in Environmental Biology, 5: 429 Protocols for In Vitro Cultures and Secondary and Bioallied Sciences, 3: 479-495. Miguel M.G., Virgin olive oil: composition and -432. Metabolite Analysis of Aromatic and Medicinal Miguel M.G., S. Nunes, S.A. Dandlen, A.M. biological properties. In Fixed oils and fats of Dandlen S.A., M.G. Miguel, J.M. Duarte, M.L. Plants. S. Mohan Jain and Praveen K. Saxena Cavaco, M.D. Antunes, Antioxidant Activity of pharmaceutical importance. Recent Progress in Faleiro, M.J. Sousa, A.S. Lima, A.C. Figueire- (Eds), Humana Press, ISBN 978-1-60327-286-5. Propolis from Algarve. Advances in Environmen- Medicinal Plants. Vol. 33, chapter 10, Studium do, L.G. Pedro, J.G. Barroso, Acetylcholinester- Flavour Fragrance Journal, 26: 70. tal Biology, 5: 345-350. Press LLC, USA. Pp. 287-322. ase inhibition activity of Portuguese Thymus spe- Gago C., A.R. Sousa, M. Juliao, G. Miguel, D. cies essential oils. Journal of Essential Oil Bear- Antunes, T. Panagopoulos, Sustainable use of Neves M.A., M.G. Miguel, L.G. Pedro, J.G., ing Plants, 14: 140-150. energy in the storage of halophytes used for Barroso, A.C. Figueiredo, D.S. Martins, S.A. Dandlen, Variability of olive oil on stabil- Dandlen S.A., A.S. Lima, M.D. Mendes, M.G. food. International Journal of Energy and Envi- ity during storage. Acta Alimentaria, 40: 436-448. Miguel, M.L. Faleiro, M.J. Sousa, L.G. Pedro, ronment, 5: 592-599. J.G. Barroso, A.C. Figueiredo, Antimicrobial Mendes M.D., A.S. Lima, H. Trindade, A.I.D. Palavra A.M.F., J.P. Coelho, J.G. Barroso, A.P. Correia, J.G. Barroso, L.G. Pedro, A.C. Rauter, J.M.N.A. Fareleira, A. Mainar, J.S. Figueiredo, ISSR molecular characterization and Urieta, B.P. Nobre, L. Gouveia, R.L. Mendes, leaf volatiles analysis of Pittosporum undulatum J.M.S. Cabral, J.M. Novais, Supercritical carbon Vent. naturalized in the Azores archipelago dioxide extraction of bioactive compounds from (Portugal). Industrial Crops and Products, 33: microalgae and volatile oils from aromatic plants. 710-719. Journal of Supercritical Fluids, 60: 21-27. Miguel G., C. Cruz, M.L. Faleiro, M.T.F. Pestana M, I. Domingos, F. Gama, S. Dandlen, Simões, A.C. Figueiredo, J.G. Barroso, L.G. M.G. Miguel, J.C. Pinto, A. de Varennes, P.J. Pedro, Salvia officinalis L. essential oils: effect of Correia, Strawberry recovers from iron chlorosis hydrodistillation time on the chemical composi- after foliar application of a grass-clipping extract. tion, antioxidant and antimicrobial activities. Nat- Journal of Plant Nutrition and Soil Science, 174: ural Product Research, 25: 526–541. 473-479. Miguel M.G., Anthocyanins: Antioxidant and/or Smail A., L. Badia, M.G. Miguel, Antioxidant anti-inflammatory activities. Journal of Applied and antiacetylcholinesterase activities of some Pharmaceutical Science, 1: 7-15. Other Communications Feijão M.D., G. Teixeira, A.I. Correia, L.G. Pe- dro, J.G. Barroso, A.C. Figueiredo (2011) Es- International Conferences sential oils from Lavandula luisieri (Rozeira) Ri- vas-Martinez grown at a montado ecosystem. Aazza S., B. Lyoussi, M.G. Miguel, A.C. 42th International Symposium on Essential Oils, Figueiredo, L.G. Pedro, J.G. Barroso (2011) Antalya, Turkey, p. 178. Antioxidant and acetylcholinesterase inhibitory Feijão M.D., G. Teixeira, T. Vasconcelos, L. activity from Citrus aurantium L., Citrus limon Rodrigues, A.I. Correia, J. Sanches, L.G. Pe- (L.) Burm. f., Cupressus sempervirens L., Euca- dro, J.G. Barroso, A.C. Figueiredo (2011) Es- lyptus globulus Labill., Foeniculum vulgare Mill. sential oil variability and trichomes morphology and Thymus vulgaris L. Moroccan essential from Lavandula pedunculata (Mill.) Cav. grown oils). 42th International Symposium on Essential at Mata Experimental do Escaroupim (Portugal). Oils, Antalya, Turkey, p. 72. 59th International Congress and Annual Meeting Anas E., A. El Amrani, J.J. Eddine, J.G. Bar- of the Society for Medicinal Plant and Natural on Energy & Environment, Cambridge, UK, pp. roso, L.G. Pedro, A.C. Figueiredo (2011) Yield Product Research (GA 2011), Antalya, Turkey, 23-25. and chemical composition of the essential oil with an updated literature survey, Fourth Inter- p. 1297. from Ormenis mixta grown in Morocco. 4ème national Chemical Conference, Riyadh, Saudi- Leitão C.A.E., L. Ascensão (2011) Structure, Figueiredo A.C., L.G. Pedro, J.G. Barroso Symposium International Plantes Aromatiques e Arabia, p. 146. development and histochemistry of the bracteal (2011) Hairy root cultures and the in vitro pro- Médicinales (SIPAM), Mohammedia, Morocco, Falcão S.I., A.C. Figueiredo, M. Vilas-Boas and receptacular colleters of Encyclia alboxan- duction of volatiles: an update. 42th Internatio- p. 160. (2011) Volatile composition of Northeast Portu- thina (Orchidaceae). 18th International Botanical nal Symposium on Essential Oils, Antalya, Tur- Antunes M.D.C., D.A.C. Rodrigues, A.M. Ca- guese propolis. 1º Congresso Ibérico de Apicul- Congress, Melbourne, Austrália. key, p. 32. vaco, M.G. Miguel (2011) Evaluation of Quality tura, Castelo Branco, Portugal, pp. 89-90. Lima A.S., B. Lukas, J. Novak, A.C. Figueire- Changes during Shelf-life in Minimally Pro- Figueiredo A.C., L.G. Pedro, J.G. Barroso Falé P.L.V., M.A. Filipe, L. Ascensão, M.L.M. do, L.G. Pedro, J.G. Barroso, H. Trindade cessed Kiwifruit. Acta Horticulturae, 913: 553- (2011) Volatile phytochemicals production by Serralheiro, L. Mira (2011). Activity of Plectran- (2011) Genomic characterization of γ-terpinene 557. hairy root cultures. Plant Engine – COST Action thus barbatus extract against inflammatory re- synthase from Thymus caespititius. 59th Inter- FA 1006, Murcia, Spain, p. 70. Bahcevandziev K., A.S. Lima, H. Trindade sponse in human neutrophils. International PSE national Congress and Annual Meeting of the (2011) -Estimation of nuclear DNA content in Symposium - Phytochemicals in Nutrition and Francisco A., L. Ascensão (2011). Osmopho- Society for Medicinal Plant and Natural Product various chemotypes of Thymus caespititius Brot. Health, Bari, Itália. res in the labellum of three Ophrys species: Research (GA 2011), Antalya, Turkey, p. 1361. (Lamiaceae) by flow cytometry, 1st Annual Con- structure and role in pollination of sexually de- Faria J.M.S., A.C. Figueiredo (2011) Compara- Lima S., J. Novak, M. Mendes, A.C. Figueire- ference, Plant Engine, Cost Meeting, Action ceptive orchids.18th International Botanical tive evaluation of the volatiles from micropropa- do, L. Pedro, J. Barroso, H. Trindade (2011) FA1006, Murcia, Spain, p: 28 Congress, Melbourne, Austrália. gated and two-years-old field grown Pinus pi- The molecular basis for chemotypes in Thymus Barbosa P., J.M.S. Faria, M.D. Mendes, L.S. naster and Pinus pinea plants. 7th International Francisco A., L. Ascensão (2011). Structure caespititius, 1st Annual Conference, Plant En- Dias, J.G. Barroso, L.G. Pedro, A.C. Figueire- Symposium on In Vitro Culture and Horticultural and function of the osmophores in three species gine, Cost Meeting, Action FA1006, Murcia, do, M. Mota (2011) Search for pinewood nema- Breeding, Ghent, Belgium, p. 43-44. of Ophrys, a genus of sexually deceptive polli- Spain, p: 43. tode control using essential oils isolated from Faria J.M.S., P. Barbosa, M. Mota, J. nated orchids. 2nd Joint Congress of Portugue- Portuguese aromatic flora. XLIII ONTA Annual Lopes V.R, A.M. Barata, F. Rocha, L.G. Ped- Sanches, A.C. Figueiredo (2011) Evaluation of se and Spanish Microscopy Societies, Aveiro, Meeting, Coimbra, Portugal, p. 160. ro, J.G. Barroso, A.C. Figueiredo (2011) Mor- the nematicidal activity against the pinewood Portugal. phological and chemical variability assessment Belhattab R., L. Amor, J.G. Barroso, L.G. nematode, Bursaphelenchus xylophilus, of es- Gago C., A.R. Sousa, M. Juliao, G. Miguel, D. from Portuguese Mentha pulegium L. Pedro, A.C. Figueiredo (2011) Essential oil sential oil-bearing plants from Portuguese flora. Antunes, T. Panagopoulos (2011) Refrigerati- (pennyroyal) accessions. Proceedings of the VIII from Artemisia herba-alba Asso grown wild in XLIII ONTA Annual Meeting, Coimbra, Portugal, on Needs for Sustainable Preservation of Horti- International Ethnobotany Symposium, Lisboa, Algeria: variability assessment and comparison p. 162. cultural Products. 6th International Conference Portugal, p. 573-583. Other Communications

International Conferences (cont) Lima A.S., C. Oliveira, L. Domingues, H. Trin- qualidade dos produtos hortícolas produzidos na dade (2011) Isolation and molecular cloning of Póvoa de Varzim”, Mestrado em Hortofruticultura Marin M., L. Ascensão, S. Duletic-Lausevic, γ terpinene synthase gene from Thymus caespi- (Universidade do Algarve). Supervisors: Maria P.A. Marin (2011) Light and electron microscopy titius, MicroBiotec Congress, Braga, p: 275. Dulce Carlos Antunes (CBV/IBB, UALG) e Maria in investigation of glandular trichomes of Satureja Raquel Lucas (UE). Rodrigues A.M., P.L.V. Falé, L. Ascensão, A.R. montana L. 10th Multinational Congress on Mi- Lino, M.L.M. Serralheiro (2011). Actividades Maria Inês Moreira Figueiredo Dias, croscopy (MCM 2011), Urbino, Itália. biológicas de três espécies da flora aromática “Caracterização química e molecular de amostras Mendes M.D., A.C. Figueiredo, M.M. Oliveira, Portuguesa. 1º Encontro Ciência do Centro de de Coriandrum sativum L. obtidas in vivo e in H. Trindade (2011) Expression of a putative Química e Bioquímica, Lisboa, Portugal. (winner vitro”, Mestrado em Biotecnologia (Escola Superi- monoterpene synthase along Thymus caespititius of the best poster award). or Agrária de Bragança). Supervisors: Maria João Sousa e Isabel Cristina Ferreira (ESA) e Helena micropropagation. 7th International Symposium Pedro L.G., A.C. Figueiredo, J.G. Barroso Trindade (CBV/IBB, FCUL). on In Vitro Culture and Horticultural Breeding, (2011) Métodos de obtenção dos óleos essen- Ghent, Belgium, p. 63. ciais, V Curso de Plantas Aromáticas e Medici- Marta Taveira Santos Castro Silva, “Avaliação nais em Terras do Pulo do Lobo, Mértola. da capacidade de extractos voláteis de plantas Mota L., A.C, Figueiredo, L. Pedro, J. Barroso, Burm. f., Eucalyptus globulus Labill., Foeniculum aromáticas para inibir a formação de biofilmes M.G. Miguel, L. Faleiro, L. Ascensão (2011) vulgare Mill. and Thymus vulgaris. 42nd Interna- bacterianos”, Mestrado em Biologia Celular e Chemical composition, antioxidant and antimicro- tional Symposium on Essential Oils, Antalaya, PhD. Thesis Biotecnologia Vegetal (FCUL). Supervisors: Carla bial activities of volatiles from Plectranthus barba- Turquia da Conceição Caramujo Rocha de Carvalho tus, P. neochilus and P. ornatus (Lamiaceae). Dayana Maria Teodoro Francino, “Anatomia (BERG/IBB, IST) e Ana Cristina Figueiredo (CBV/ 42th International Symposium on Essential Oils, National Conferences foliar de Chamaecrista Moench. (Leguminosae- IBB, FCUL) Antalya, Turkey, p. 129. Caesalpinioideae) como subsídio à taxonomia e Bahcevandziev K., R. Guilherme, F. Melo, P. à filogenia”. Supervisors: Lia Ascensão, (CBV, Natacha Sofia Greno de Moura, “Usos tradicio- Panagopoulos T., M.D.C. Antunes (2011) Spa- Mota, H. Trindade (2011) Estudo preliminar de FCUL, UL), Renata Meira and Aristéa Alves nais e avaliação da composição química dos cial distribution of qualitative characteristics and quimiotipos de Thymus caespititius com recurso Azevedo (DB, Federal Univ of Viçosa, MG, Bra- óleos essenciais de plantas aromáticas e medici- production factors of kiwifruit at “Beira Litoral”, à técnica da citometria de fluxo, 3º Colóquio sil). nais utilizadas em Lisboa e Bragança”, Mestrado Portugal. Acta Horticulturae, 913: 609-614. Nacional de Horticultura Biológica (Braga), pp: 42 em Biologia Celular e Biotecnologia Vegetal (FCUL). Supervisors: Ana Carvalho (Instituto Panagopoulos T., C. Gago, A.R. Sousa, M. Bahcevandziev K., R. Guilherme, F. Melo, P. M.Sc. Thesis Politécnico de Bragança), Ana Cristina Figueire- Juliao, G. Miguel, M.D.C. Antunes (2011) Ex- Mota, H. Trindade (2011) Estudo preliminar de Ana Margarida Rodrigues, “Actividades Biológi- do (CBV/IBB, FCUL) ploiting halophytes from Ria Formosa (Portugal) quimiotipos de Thymus caespititius com recurso cas e Estruturas Secretoras em Três Espécies de for fresh salads. 2nd Conference of COST Action à técnica da citometria de fluxo, Actas Portu- Asteraceae da Flora Aromática Portuguesa”. FA0901 ‘Ecology of halophytes and saline habi- guesas de Horticultura, 17: 48-51 Mestrado em Biologia Celular e Biotecnologia tats’, Torun, Poland. Dias, M.I., I. Ferreira, H. Trindade, M.J. Sousa (FCUL). Supervisors: Luísa Serralheiro (CQB, Rodrigues A.M., P.L.V. Falé, L. Ascensão, (2011) Caracterização química e molecular de FCUL), Lia Ascensão (CBV/IBB, FCUL) M.L.M. Serralheiro (2011). Acetylcholinesterase amostras de Coriandrum sativum L. obtidas in Inês Correia dos Santos Carvalho de Sena, inhibition and antioxidant activity of three species vivo e in vitro. I Jornadas de Jovens Investiga- “Avaliação da diversidade genética de popu- from the Portuguese Aromatic Flora. International dores da Escola Superior Agrária de Bragança, lações de Tuberaria major usando marcadores PSE Symposium - Phytochemicals in Nutrition Nov.11-12. de ISSR”. Mestrado Integrado em Engenharia and Health, Bari, Itália. Falé P.L.V., L. Ascensão, M.L.M. Serralheiro Biológica (Universidade do Algarve). Supervisors: Smail A., L. Badiâa, M.G. Miguel, A.C. (2011). Plectranthus barbatus no tratamento da Anabela Romano (CGB/IBB, UAlg) e Helena Trin- Figueiredo, L.G. Pedro, J.G. Barroso (2011) doença de Alzheimer. 1º Encontro Ciência do dade (CBV/IBB, FCUL). Antioxidant and acetylcholinesterase inhibitory Centro de Química e Bioquímica, Lisboa, Portu- Inês Margarida Pereira Ramos, “Perceção da activity from Citrus aurantium L., Citrus limon (L.) gal.