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TERPNET 2013 2 TERPNET 2013 TERPNET 2013 11th International meeting Biosynthesis, Function and Biotechnology of Isoprenoids in Terrestrial and Marine Organisms A joint Meeting of TERPNET2013 Cost Action “Plant Engine” Cost Action “QualityFruit” “EU-SmartCell” June 1-5, 2013, Kolymvari, Crete, Greece Department of Pharmaceutical Science Aristotle University of Thessaloniki 3 TERPNET 2013 Contents TERPNET 2013 - Invitation and Introduction .......................................................................... 5 Terpnet Organization ................................................................................................................... 5 Local Organizing Committee .............................................................................................................. 7 Executive Committee of TERPNET ................................................................................................... 7 Financial support-Sponsors ......................................................................................................... 8 Program for TERPNET 2013 .............................................................................................................. 9 Abstracts and Oral presentations .............................................................................................. 29 List of Participants .................................................................................................................... 257 Index of Names .......................................................................................................................... 263 4 TERPNET 2013 TERPNET 2013 - Invitation and Introduction On behalf of TERPNET 2013, COST Action FA1006 “Plant Engine: Plant Metabolic Engineering”, COST Action FA1106 “QualityFruit” and SmartCell we are pleased to welcome you in 11th TERPNET meeting taking place June 1-5, 2013 in Kolymvari, Crete, Greece. Terpnet is an international affiliation of researchers working on all aspects of terpenes and isoprenoids, including investigations spanning from biosynthetic mechanisms to assessing the biological activities of these chemicals. This affiliation hosts bi-annual meetings that have been held in Europe, USA and Japan for the past two decades. The 10th meeting (TERPNET2011) was held in Kalmar, Sweden May 2011 and was organized by Professor Peter Brodelius, Linnaeus University. The 2013 meeting is organized by the Local Committee headed by Prof. Angelos Kanellis in close collaboration with the Executive Committee. Exceptionally, this event will be co-organized with COST Action FA1006 “Plant Engine”, COST Action FA1106 “QualityFruit” and EU project SmartCell. Cost Action FA1006 “Plant Engine” supports and enhances a Pan-European network, which combines resources, identifies target pathways and defines compounds, publicizes novel technologies and applications, sets standards for computational support, and develops synthetic approaches in plant metabolic engineering. The aim of COST action FA1106 “QualityFruit” is to foster European collaborations on fleshy fruits, such as tomato and grapevine. The main objective is to uncover new leads for improving fruit nutritional and sensory qualities by implementing integrated multidisciplinary approaches and advanced genomics and post-genomics technologies. Lastly, SmartCell is an EU Seventh Framework Program large collaborative project investigating the rational design of plant systems for the sustainable generation of value-added industrial products. In addition, prior to the main conference, the Final Meetings of two EU projects dealing with the biotechnology of plant secondary metabolites will take place, namely SmartCell and TERPMED. The scientific program of TERPNET 2013 covers all aspects of terpene/isoprenoid research including biosynthesis and structural biology, transport and regulation, genetics, metabolic engineering and biotechnology, synthetic biology applications as well as applications in agriculture and medicine. Plants, microorganisms, parasites and marine organisms are represented. The meeting takes place in small traditional village, Kolymvari, near Chania, Crete. The venue, the Orthodox Academy of Crete (OAC) is a modern Conference Center, situated in an 5 TERPNET 2013 exceptionally beautiful location only a few meters from the sea, and next to the Gonia Monastery (17th Century). We would like to thank Cost Actions FA1006 and FA1106 for sponsoring and co-organizing TERPPET2013. We are particularly indebted to the members of the TERPNET2013 Executive Committee and the local organizing committees as well as to the staff of the OAC Conference site for their efforts for the success of this Symposium. Appreciation is also extended to a number of private firms, which contributed to the success of this important event. Lastly, we acknowledge the help of the Group of Biotechnology of Pharmaceutical Plants of the Dept. of Pharmaceutical Science, Aristotle University. We wish you a pleasant stay in Kolymvari and an enjoyable TERPNET2013 Meeting. Angelos K. Kanellis, Heribert Warzecha, Mondher Bouzayen and Kirsi-Marja Oksman- Caldentey. 6 TERPNET 2013 Terpnet Organization Angelos K. Kanellis (Aristotle University of Thessaloniki, Greece - Chairman) Heribert Warzecha (Technische Universitaet Darmstadt, Germany) Mondher Bouzayen (UMR990 INRA/INP-ENSAT, France) Kirsi-Marja Oksman-Caldentey (VTT Technical Research Centre, Finland) Local Organizing Committee Vassilios Roussis (Kapodistrinian and National University of Athens) Kaliope Papadopoulou (University of Thessaly) Frangiskos Kolisis (National Technical University of Athens) Sotirios Kampranis (University of Crete) Antonios Makris (Institute of Agrobiotechnology/CERTH) Executive Committee of TERPNET Thomas J. Bach (University of Strasbourg, France) Jörg Bohlmann (University of British Columbia, Canada) Albert Boronat (University of Barcelona, Spain) Harro Bouwmeester (Wageningen University and Research Center, The Netherlands) Peter Brodelius (Linnaeus University, Sweden) Joe Chappell (University of Kentucky, USA) Jonathan Gershenzon (Max Planck Institute for Chemical Ecology, Germany) Werner Knöss (University of Bonn, Germany) Toshiya Muranaka (Yokohama City University, Japan) Joe Noel (Salk Institute for Biological Studies, USA) Anne Osbourn (John Innes Centre, UK) Reuben J. Peters (Iowa State University, USA) Eran Pichersky (University of Michigan, USA) 7 TERPNET 2013 Sponsors Lab Supplies Scientific BIOLine ® S c i e n t i f i c 8 TERPNET 2013 Program Outline of TERPNET 2013 Time Fri 31 May Sat 1 June Sun 2 June Mon 3 June Tue 4 June Wed 5 June Thu 6 June Session Session V VIII Terpenoids Session IX Terpenoids Roles in Fleshy Natural and Fruit Biology Roles for Pre- beyond Post- (sponsored by Terpenoids Symposium sponsored Symposium COST Action Introduction 8-9 Tour Official Opening by COST Tour FA1106 Jonathan Samara ACTION Samaria QUALITYFRUIT) Gershenzon Gorge Plant Gorge Mondher Dorothea Engine Bouzayen Tholl Heribert Jossi Hirschberg John Pickett Warzecha Jules Beekwilder Paul Fraser Session II Terpenoids and Pharmacognocy Matthias st Nees Rob Kalliopi in 21 Century Asaph Aharoni 9-10 Verpoorte Papdopoulou Werner Knöss Harry Klee Ludger Selected Talk Hermann Wessjohann Stuppner Reuben Peters Session III Exploring genomes, transcriptomes, Coffee Selected Talk proteomes and Gianfranco Diretto Break Selected Talk 10-11 Smart Cell metabolome to Stella Grando Efraim Selected Talk understand the Lewinsohn Coffee Break Meeting terpenome Philipp Zerbe Eric Schmelz Coffee Break Session X Elio Fantini TERPMED Session VI Plant and Johann FINAL Marine microbial Anders PROJECT Coffee Break terpenoids: production Rettberg 11-12 Meeting Joerg Bohlmann chemical systems for Benoit Kazuki Saito diversity, terpenoids Boachon function and Alain Dong biotechnology Goossens Lemeng Ernesto Mollo Paul Christou Selected Talk Vassilios Roussis Antonios 12-13 Selected Talk Shigeru Okada Lunch Makris Selected Talk Selected Talk Anneli Ritala 13-14 Lunch Lunch Lunch Session VII Session IV Understanding Terpenoids of structure and Toshiya biosynthesis function of Excursion Muranaka 14-15 and regulation terpene Selected Talk Albert Ferrer synthases Selected Talk Sarh O’ Connor Francis Mann Tariq Akhtar 9 TERPNET 2013 Selected Talk Selected Talk Qing Liu Selected Talk 15-16 Selected Talk Drik Pruefer Selected Talk Coffee Break Coffee Break Session XI New and Old Natalia Dudareva Feng Chen Approaches Coffee Break Sotirios Christina 16-17 Harro Kampranis White Bouwmeester Brigit Piechulla Joe Noel Martin Burke Selected Talk Selected Talk Session XII Terpenoids Registration and Selected Talk Industrial Selected Talk Selected Talk Applications 17-18 Selected Talk Selected Talk Michel Schalk Selected Talk Selected Talk Richard Burlingame Selected Talk Selected Talk Selected Talk General Selected Talk Selected Talk Discussion 18-19 Special Lecture I Selected Talk and Peter Brodersen Selected Talk Conclusion Session I Introduction to the Symposium Selected Talk Poster 19-20 Poster Session II Eran Poster Session I Session Pichersky Thomas Calikowski 20-21 Wine Tasting Dinner Dinner Farewell 21-22 and Welcome Dinner Dinner Gala Dinner 22-23 23-24 10 TERPNET 2013 Connor Program for TERPNET 2013 A joint Meeting of TERPNET2013, Cost Action “Plant Engine”, Cost Action QualityFruit” and “EU-SmartCell” Biosynthesis, Function and Biotechnology of Isoprenoids in Terrestrial and Marine Organisms Saturday June 1, 2013 16:00-19:00 Registration Session I 19:00-20:10 Introduction
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  • Morphological, Molecular and Phytochemical Variation in Some Thyme Genotypes

    Morphological, Molecular and Phytochemical Variation in Some Thyme Genotypes

    Bulletin of Environment, Pharmacology and Life Sciences Bull. Env. Pharmacol. Life Sci., Vol 5 [11] October 2016: 25-35 ©2015 Academy for Environment and Life Sciences, India Online ISSN 2277-1808 Journal’s URL:http://www.bepls.com CODEN: BEPLAD Global Impact Factor 0.533 Universal Impact Factor 0.9804 ORIGINAL ARTICLE OPEN ACCESS Morphological, Molecular and phytochemical Variation in Some Thyme Genotypes Marzieh Dalir1 and Abbas Safarnejad* 1MSc, Biotechnology,Mashhad Branch, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, Iran. Email:[email protected] 2*Corresponding author, Associate Professor, Faculty member of Khorasan Razavi Agricultural and Natural Resources Research Center or Mashhad Branch, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, Iran Email: [email protected], ABSTRACT Thyme is an important medicinal plant in cosmetic, pharmaceutical and food industries. The first step for breeding of thyme is evaluating of genetic variation and relationship between thyme’s accessions. Thirteen accessions of Thyme medicinal plant were studied in the aspect of morphology, chemical and molecular variation. ANOVA showed significant differences between accessions for total characterization tested. The dendrogram constructed on the basis of morphology similarities showed two major clusters. In order to evaluate the genetic variation, the genomic DNA extracted using modified medicinal CTAB protocol. The evaluation of the quality of DNA was examined with electrophoresis. Twenty primers were used for PCR analysis and only 9 primers showed clear bands. Out of 149 bands, 83/22% were the polymorphism. The data were analyzed with SPSS and POPGENE programs and the dendrogram was drawn based on UPGMA and showed three major clusters.