DOCSLIB.ORG

Molecular Interactions of Colicin Fy with a Susceptible Bacterial Cell

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Molecular Interactions of Colicin Fy with a Susceptible Bacterial Cell MASARYK UNIVERSITY faculty of medicine department of biology BACTERIOCINOGENY OF YERSINIAE: MOLECULAR INTERACTIONS OF COLICIN FY WITH A SUSCEPTIBLE BACTERIAL CELL Brno, 2013 Juraj Bosák BIBLIOGRAPHIC IDENTIFICATION Name and Surname: Juraj Bosák Title 0f Thesis: Bacteriocinogeny of yersiniae: Molecular interactions of colicin FY with a susceptible bacterial cell Study Programme: Medical Biology 5103V022 Supervisor: doc. MUDr. David Šmajs, Ph.D. Defended in: 2013 Keywords: Antibacterial toxin, Bacteriocin, Colicin, Yersinia, Y. enterocolitica, Escherichia, Yersiniosis, Probiotics 6 ABSTRACT There are numerous antimicrobial agents that are produced by bacteria. The role of these substances is to improve fitness of the producer in a daily fight for survival. Bacteriocins are substances that inhibit growth of other bacteria. One important subgroup of bacteriocins is represented by colicins, antimicrobial agents produced by colicinogenic strains of Escherichia coli and other related species of the family Enterobacteriaceae. These toxins specifically inhibit closely related bacteria based on the presence of a specific receptor on the cell surface. Genus Yersinia comprises important human pathogens such as Y. pestis, Y. pseudotuberculosis, and Y. enterocolitica. Growth inhibition between various strains of yersiniae is quite common. Out of numerous identified bacteriocin-like substances produced by yersiniae, only two, pesticin I and enterocoliticin, were characterized in detail. In fact, these are the only two characterized bacteriocins known to attack yersiniae. Therefore, a search for fully characterized bacteriocins that act specifically against yersiniae has been demanded. In this study, we describe a novel colicin - colicin FY - isolated from a colicinogenic strain of Ye- rsinia frederiksenii; we show its complete plasmid sequence (pYF27601), mechanism of its toxicity, corresponding receptor (YiuR), and translocation routes into a susceptible bacterium. This is the first well characterized colicin, which is active mainly against strains of Y. enterocolitica. The universal susceptibility of Y. enterocolitica to colicin FY together with absence of activity on strains outside the genus Yersinia suggests potential therapeutic applications of colicin FY. 7 ABSTRAKT Bakterie produkují různé antimikrobiální látky, které je zvýhodňují vůči jiným bakteriím v každo- denním boji o přežití. Koliciny jsou významnou skupinou bakteriocinů; jsou produkovány některý- mi kmeny druhu Escherichia coli a příbuznými druhy čeledě Enterobacteriaceae. Koliciny se vyzna- čují inhibičním účinkem vůči příbuzným bakteriím, který je dán přítomností specifického receptoru na povrchu citlivé bakterie. U rodu Yersinia, zahrnující patogenní druhy Y. pestis, Y. pseudotuberculosis a Y. enterocolitica, byla popsána produkce různých antibakteriálních substancí, avšak pouze dvě – pesticin I a enterokoliticin - byly detailně charakterizovány. V současnosti jsou to jediné dva detailně charakterizované bakteri- ociny, které účinkují vůči yersiniím. Tato práce se zaměřila na charakterizaci nově objeveného antimikrobiálního agens produko- vaného nepatogenním kmenem Yersinia frederiksenii. Jedná se o nový typ kolicinu, který byl poj- menován kolicin FY. Byla získána kompletní sekvence plazmidu produkujícího kolicin FY a současně byl popsán mechanizmus účinku kolicinu FY. Protein YiuR byl identifikován jako receptor kolicinu FY. Kolicin FY se vyznačuje univerzálním účinkem vůči kmenům Y. enterocolitica. Současně neby- la pozorována inhibice jiných bakterií mimo rod Yersinia, což naznačuje potenciální terapeutické využití kolicinu FY. DECLARATION I hereby declare that I have worked independently, using only the primary and secondary sources listed in the bibliography, under the supervision of doc. MUDr. David Šmajs, Ph.D. .......................................................................... In Brno Mgr. Juraj Bosák ACKNOWLEDGEMENTS I would like to thank my supervisor doc. MUDr. David Šmajs, Ph.D. for his ideas, support, and pa- tience. Special thank belongs to Petra Laiblová (Kotrsalová). She started this project and in fact discovered the new bacteriocin. My objective has been characterization of her discovery. Many thanks also belong to my colleagues from the Department of Biology, namely Lenka Micen- ková, Lenka Mikalová, Michal Strouhal, and Marie Zobaniková for always being here for me. Míša, thank you for your love and understanding during the years spent at The University. Your sup- port allowed finishing my studies. This study was supported by a grants from the Ministry of Health of the Czech Republic (NS9665-4/2008, NT13413-4/2012) and by institutional support from the Czech Republic (MSM0021622415) CONTENTS I. THEORETICAL PART .........................................................17 1. Theoretical background ........................................................19 1.1. Bacteriocins ................................................................19 1.2. Colicins ...................................................................20 1.2.1. Classification .................................................................20 1.2.2. Genetic organization ..........................................................22 1.2.3. Release from the producing cell .................................................24 1.2.4. Structure .....................................................................24 1.2.5. Mechanism of action ..........................................................27 1.2.5.1. Colicin interactions with the outer membrane ...................................27 1.2.5.1.1. Interaction partners in the outer membrane ...................................27 TonB-dependent transporters (TBDTs) ......................................27 Outer membrane porins ...................................................28 1.2.5.1.2. Interaction of colicins with primary receptors ..................................29 1.2.5.1.3. Interaction of colicins with secondary receptors (translocators) ...................31 Translocon of Tol-dependent colicins ........................................31 Translocon of TonB-dependent colicins ......................................32 1.2.5.2. Colicin interactions in the periplasmic space ....................................33 1.2.5.2.1. Interaction partners in the periplasmic space ..................................33 1.2.5.2.2. Interaction of colicins with the translocation system ............................34 1.2.5.3. Colicin interactions with the inner membrane ...................................35 1.2.5.3.1. Interaction of pore-forming colicins with the inner membrane ...................35 1.2.5.3.2. Interaction of enzymatic colicins with the inne membrane .......................36 1.2.5.4. Colicin interactions in the cytoplasm ...........................................37 II. EXPERIMENTAL PART ......................................................39 2. Specific aims .................................................................41 3. Materials and methods .........................................................43 3.1. Bacterial strains ................................................................43 3.2. Culture media .................................................................43 3.3. Detection of the colicin production ...............................................43 3.4. Preparation of crude colicin extracts ..............................................45 3.5. Colicin activity assay ............................................................45 3.6. Isolation of plasmids ............................................................45 3.7. PCR product purification ........................................................45 3.8. Preparation of chemocompetent cells and transformation ............................45 3.9. Preparation of electrocompetent cells and transformation ............................45 3.10. Plasmid in vitro mutagenesis and construction of plasmid library ....................46 3.11. DNA sequencing and sequence analysis. .46 3.12. Construction of the genome library ..............................................47 3.13. Chromosomal mutagenesis in vivo ...............................................47 3.14. Identification of receptor for colicin FY ...........................................47 3.15. DNA cloning. .47 3.16. Colicin purification and immunoblot analysis .....................................48 3.17. Channel-forming assay .........................................................48 3.18. 16S rRNA analysis .............................................................48 3.19. Bioserotype classification .......................................................49 3.20. Pulsed field gel electrophoresis ..................................................49 3.21. Detection of virulence factors ...................................................49 3.22. Antibiotic susceptibility assay ...................................................50 4. Results and discussion .........................................................51 4.1. Bacteriocin production in the genus Yersinia .......................................51 4.2. Activity spectrum of the bacteriocin of Y. frederiksenii 27601 .........................53 4.3. Isolation and sequencing of the colicinogenic plasmid YF27601 .......................54 4.4. Analysis of the colicinogenic region on pYF27601 ...................................56 4.5. Sequence analysis of colicin FY on the protein level ..................................57 4.6. Cloning, purification,
Load more

Recommended publications
  • UNITEL PROUDLY REPRESENTS the INTERNATIONAL TV DISTRIBUTION of Browse Through the Complete Unitel Catalogue of More Than 2,000 Titles At
    UNITEL PROUDLY REPRESENTS THE INTERNATIONAL TV DISTRIBUTION OF Browse through the complete Unitel catalogue of more than 2,000 titles at www.unitel.de Date: March 2018 FOR CO-PRODUCTION & PRESALES INQUIRIES PLEASE CONTACT: Unitel GmbH & Co. KG Gruenwalder Weg 28D · 82041 Oberhaching/Munich, Germany Tel: +49.89.673469-613 · Fax: +49.89.673469-610 · [email protected] Ernst Buchrucker Dr. Thomas Hieber Dr. Magdalena Herbst Managing Director Head of Business and Legal Affairs Head of Production [email protected] [email protected] [email protected] Tel: +49.89.673469-19 Tel: +49.89.673469-611 Tel: +49.89.673469-862 WORLD SALES C Major Entertainment GmbH Meerscheidtstr. 8 · 14057 Berlin, Germany Tel.: +49.30.303064-64 · [email protected] Elmar Kruse Niklas Arens Nishrin Schacherbauer Managing Director Sales Manager, Director Sales Sales Manager [email protected] & Marketing [email protected] [email protected] Nadja Joost Ira Rost Sales Manager, Director Live Events Sales Manager, Assistant to & Popular Music Managing Director [email protected] [email protected] CONTENT BRITTEN: GLORIANA Susan Bullock/Toby Spence/Kate Royal/Peter Coleman-Wright Conducted by: Paul Daniel OPERAS 3 Staged by: Richard Jones BALLETS 8 Cat. No. A02050015 | Length: 164' | Year: 2016 DONIZETTI: LA FILLE DU RÉGIMENT Natalie Dessay/Juan Diego Flórez/Felicity Palmer Conducted by: Bruno Campanella Staged by: Laurent Pelly Cat. No. A02050065 | Length: 131' | Year: 2016 OPERAS BELLINI: NORMA Sonya Yoncheva/Joseph Calleja/Sonia Ganassi/ Brindley Sherratt/La Fura dels Baus Conducted by: Antonio Pappano Staged by: Àlex Ollé Cat.
    [Show full text]
  • Verdi Otello
    VERDI OTELLO RICCARDO MUTI CHICAGO SYMPHONY ORCHESTRA ALEKSANDRS ANTONENKO KRASSIMIRA STOYANOVA CARLO GUELFI CHICAGO SYMPHONY CHORUS / DUAIN WOLFE Giuseppe Verdi (1813-1901) OTELLO CHICAGO SYMPHONY ORCHESTRA RICCARDO MUTI 3 verdi OTELLO Riccardo Muti, conductor Chicago Symphony Orchestra Otello (1887) Opera in four acts Music BY Giuseppe Verdi LIBretto Based on Shakespeare’S tragedy Othello, BY Arrigo Boito Othello, a Moor, general of the Venetian forces .........................Aleksandrs Antonenko Tenor Iago, his ensign .........................................................................Carlo Guelfi Baritone Cassio, a captain .......................................................................Juan Francisco Gatell Tenor Roderigo, a Venetian gentleman ................................................Michael Spyres Tenor Lodovico, ambassador of the Venetian Republic .......................Eric Owens Bass-baritone Montano, Otello’s predecessor as governor of Cyprus ..............Paolo Battaglia Bass A Herald ....................................................................................David Govertsen Bass Desdemona, wife of Otello ........................................................Krassimira Stoyanova Soprano Emilia, wife of Iago ....................................................................BarBara DI Castri Mezzo-soprano Soldiers and sailors of the Venetian Republic; Venetian ladies and gentlemen; Cypriot men, women, and children; men of the Greek, Dalmatian, and Albanian armies; an innkeeper and his four servers;
    [Show full text]
  • Geoffrey Baer, Who Each Friday Night Will Welcome Local Contestants Whose Knowledge of Trivia About Our City Will Be Put to the Test
    From the President & CEO The Guide The Member Magazine Dear Member, for WTTW and WFMT This month, WTTW is excited to premiere a new series for Chicago trivia buffs and Renée Crown Public Media Center curious explorers alike. On March 26, join us for The Great Chicago Quiz Show hosted by 5400 North Saint Louis Avenue Chicago, Illinois 60625 WTTW’s Geoffrey Baer, who each Friday night will welcome local contestants whose knowledge of trivia about our city will be put to the test. And on premiere night and after, visit Main Switchboard (773) 583-5000 wttw.com/quiz where you can play along at home. Turn to Member and Viewer Services page 4 for a behind-the-scenes interview with Geoffrey and (773) 509-1111 x 6 producer Eddie Griffin. We’ll also mark Women’s History Month with American Websites wttw.com Masters profiles of novelist Flannery O’Connor and wfmt.com choreographer Twyla Tharp; a POV documentary, And She Could Be Next, that explores a defiant movement of women of Publisher color transforming politics; and Not Done: Women Remaking Anne Gleason America, tracing the last five years of women’s fight for Art Director Tom Peth equality. On wttw.com, other Women’s History Month subjects include Emily Taft Douglas, WTTW Contributors a pioneering female Illinois politician, actress, and wife of Senator Paul Douglas who served Julia Maish in the U.S. House of Representatives; the past and present of Chicago’s Women’s Park and Lisa Tipton WFMT Contributors Gardens, designed by a team of female architects and featuring a statue by Louise Bourgeois; Andrea Lamoreaux and restaurateur Niquenya Collins and her newly launched Afro-Caribbean restaurant and catering business, Cocoa Chili.
    [Show full text]
  • Methanococcoides Burtonii: the Role of the Hydrophobic Proteome and Variations in Cellular Morphology
    Cold Adaptation in the Antarctic Archaeon Methanococcoides burtonii: The Role of the Hydrophobic Proteome and Variations in Cellular Morphology Thesis submitted in partial fulfilment of the requirements for the Degree of Doctor of Philosophy (Ph.D.) Dominic W. Burg School of Biotechnology and Biomolecular Sciences University of New South Wales 2009 THE UNIVERSITY OF NEW SOUTH WALES Thesis/Dissertation Sheet Surname or Family name: Burg First name: Dominic Other name/s: William Abbreviation for degree as given in the University calendar: PhD School: Biotechnology and Biomolecular sciences Faculty: Science Title: Cold adaptation in the Antarctic archaeon Methanococcoides burtonii: The role of the hydrophobic proteome and variations in cellular morphology Abstract Very little is known about the hydrophobic proteins of psychrophiles and their roles in cold adaptation. In light of this situation, methods were developed to analyse the hydrophobic proteome (HPP) of the model psychrophilic archaeon Methanococcoides burtonii. Central to this analysis was a novel differential solubility fractionation procedure, which resulted in a significant increase in the efficiency of resolving the HPP. Over 50% of the detected proteins were not identified in previous whole cell extract analyses, and these underwent an intensive manual annotation process producing high quality functional assignments. Utilising the functional assignments, biological context analysis of the HPP was performed, revealing novel and often unique biology. The analysis acted as a platform for differential proteomics of the organism’s response to both temperature and substrate using stable isotope labelling. The results of which revealed that low temperature growth was associated with an increase in the abundance of surface and secreted proteins, and translation apparatus.
    [Show full text]
  • City of Penticton
    2021-04-28 City of Penticton Deceased Report Regular and Cremation - Interments and Reinterments Order by Deceased Person 1/427 Deceased Interment Date Interment Site Aanes, Bernard Christian ,AKA Kristian 1936-04-03 OS-0251-A Aasen, Hans 1972-02-28 O-099-6 Abbey, Margaret Florence (nee Hughes) 2019-01-19 L-027-7 Abbey, Robert Lee ,AKA Robert Abbey, Senior2019-11-09 L-027-7 Abbott, Dorothy 2005-06-25 N-058-4 Abbott, Francis Gilbert 1963-03-04 N-043-1 Abbott, George 2002-12-18 CS-04-54 Abbott, George K. 2007-04-14 CS-19-16 Abbott, Michael Joseph 1965-03-28 N-058-4 Abbott, Patricia Alma 2002-11-30 CS-19-16 Abbott, William Barry 1979-10-01 N-058-4 Abbott, William John 1994-05-07 N-058-4 Abel, Alexander 1949-03-21 L-014-4 Abel, Elena Winnifred 1947-05-26 L-013-1 Abel, Paul 1947-04-16 L-085-3 Abela, Marga Liesel 2012-09-15 Q-27-1 Abra, A.T. 1947-06-13 L-056-4 Abra, Amy Jane Unrecorded F-07-4 Abra, Archiena 1983-09-29 L-056-3 Abraham, Robert James Unrecorded E-04-7 Abrahamse, Bertha Nettie 1995-06-19 P-099-3 Abrahamse, Robert James Unrecorded E-04-7 Abrahamse, Stoffle Unrecorded P-099-3 Abramchuk, Nicholas Unrecorded N-053-2 Abrams, Eleanor Evelyn 1954-06-02 L-015-6 Abrams, Henry 1948-03-02 L-015-5 Abrantes, Barbara Emilia 2007-04-26 Q-35-8 Acres, Constance Charlotte Unrecorded F-09-7 Acres, Dr.
    [Show full text]
  • European Central Bank Executive Board 60640 Frankfurt Am Main Germany Brussels, 30 August 2017 Confirmatory Application to Th
    The European Parliament Fabio De Masi - European Parliament - Rue Wiertz 60 - WIB 03M031 - 1047 Brussels European Central Bank Executive Board 60640 Frankfurt am Main Germany Brussels, 30 August 2017 Confirmatory application to the ECB reply dated 3 August 2017 Reference: LS/PT/2017/61 Dear Sir or Madam, We hereby submit a confirmatory application (Art. 7 (2) ECB/2004/3) based on your reply dated 3 August 2017, in which you fully refused access to the legal opinion “Responses to questions concerning the interpretation of Art. 14.4 of the Statute of the ESCB and of the ECB”. We submit the confirmatory application on the following grounds that the ECB has a legal obligation to disclose documents based on Article 2 (1) ECB/2004/3 in conjunction with Article 15 (3) TFEU. Presumption of the exceptions set out in Article 4 (2) ECB/2004/3 (undermining of the protection of court proceedings and legal advice) and Article 4 (3) ECB/2004/3 (undermining of the deliberation process) is unlawful. 1. Protection of legal advice, no undermining of legitimate interests – irrelevance of intentions, future deliberations and ‘erga omnes’ effects In your letter you state, “In the case at hand, public release of the legal opinion – which was sought by the ECB’s decision-making bodies and intended exclusively for their information and consideration – would undermine the ECB’s legitimate interest in receiving frank, objective and comprehensive legal advice. This especially so since this legal advice was not only essential for the decision-making bodies to feed
    [Show full text]
  • August 2019 List
    August 2019 Catalogue Issue 40 Prices valid until Friday 27 September 2019 unless stated otherwise 0115 982 7500 The Wiener Staatsoper, celebrating its 150th birthday this year [email protected] Your Account Number: {MM:Account Number} {MM:Postcode} {MM:Address5} {MM:Address4} {MM:Address3} {MM:Address2} {MM:Address1} {MM:Name} 1 Welcome! Dear Customer, DG and Decca have pulled out all the operatic stops this month, with three very special releases featuring some of their best soloists. Mexican tenor Javier Camarena’s ‘Contrabandista’ recital marks the first issue in the ‘Mentored by Bartoli’ series on Decca - a partnership between Decca and the Cecilia Bartoli Music Foundation. Arias by Verdi are the focus of DG’s new recording from Russian bass, Ildar Abdrazakov, conducted by Yannick Nezet-Seguin, who also happens to take the lead in a brand new recording of Die Zauberflote, the sixth in DG’s current series of Mozart operas. It is the latter that we have been particularly taken by, so you will find it as our ‘Disc of the Month’ for August, as well as being at a very special price as part of our new DG & Decca Opera Sale (found on pp.13-18). Another recording which was a strong contender for the ‘Disc of the Month’ position, was Mahan Esfahani’s performance of Bach’s Toccatas on a rather fabulous harpsichord - Hyperion have done a sterling job in capturing Esfahani’s consummate skill in interpreting these works. Other highlights for August include the second instalment in The Illyria Consort’s recordings of sonatas by Carbonelli; hi-fi Holst and Elgar from the Bergen Philharmonic and Andrew Litton on BIS; lesser-known Beethoven from the Helsinki Baroque Orchestra, who provide us with a gripping performance of Beethoven’s complete incidental music to Egmont (Ondine); plus we have Messiaen from Tom Winpenny (Naxos), Handel from the Akademie fur Alte Musik Berlin (Pentatone), and Brahms/Dvorak from Jakub Hrusa and the Bamberger Symphoniker (Tudor), to name but a few.
    [Show full text]
  • Cross Unss Serres Castet
    CROSS UNSS SERRES CASTET Classement par équipe : Benjamin(e)s Mixtes 96 Equipes classées Dos Cat Nom Prénom Pla Points Dos Cat Nom Prénom Pla Points 20,522 542 BF BRAHIMI halima 8 2,952 569 BG LUCAS vincent 13 4,333 1 COL MARGUERITE DE NAVARRE PAU Pts 568 BG LOUSTAU mateo 10 3,333 547 BF DUBERNARD RO 16 5,904 (code de l'établissement : 4677) Bordeaux 566 BG LAPORTE arthur 12 4,000 551 BF JAEGLE lilou 26 20,987 685 BF MUGNIER alice 1 0,369 695 BG LABEGARIA timé 25 8,333 2 COL RENE FORGUES SERRES CASTET Pts 678 BF FRERE romane 3 1,107 696 BG LABORDE arthur 28 9,333 (code de l'établissement : 4685) Bordeaux 684 BF MONCADE louisa 5 1,845 690 BG DUTHIL theo 35 21,795 153 BG DUMANOWSKI el 2 0,666 154 BG ESCOURROU vic 18 6,000 3 COL DES LAVANDIERES BIZANOS Pts 134 BF GAYOT clara 10 3,690 131 BF BROUTIN lana 17 6,273 (code de l'établissement : 4658) Bordeaux 137 BF LARRIC victorine 14 5,166 145 BG ABAIJI timoe 38 21,973 1183 BG BOURDAA ilian 3 1,000 1177 BF POUYOUNE alha 12 4,428 4 COL SAINT JOSEPH NAY Pts 1201 BG PAYBOU adrien 4 1,333 1178 BF RUBIRA FLOTAT 34 12,546 (code de l'établissement : 4762) Bordeaux 1198 BG MASSE louis 8 2,666 1160 BF BALLARIN victoria 41 30,344 1242 BF MERIZ lola 4 1,476 1254 BG DUFERNEZ basil 24 8,000 5 COL SAINT DOMINIQUE PAU Pts 1258 BG LABAT GARCIA M 7 2,333 1248 BG CAMBLONG eliot 39 13,000 (code de l'établissement : 4765) Bordeaux 1243 BF MESLIN clara 15 5,535 1246 BF RAVAUX emanuel 43 39,270 347 BG CHASSAING max 20 6,666 339 BF LABAT ophelie 23 8,487 6 COL SIMIN PALAY LESCAR Pts 363 BG SEGALAS hugo 22 7,333 360
    [Show full text]
  • BEYOND COLD WAR to TRILATERAL COOPERATION in the ASIA-PACIFIC REGION Scenarios for New Relationships Between Japan, Russia, and the United States
    BELFER CENTER REPRINT NEW FOREWORD – OCTOBER 2016 BEYOND COLD WAR TO TRILATERAL COOPERATION IN THE ASIA-PACIFIC REGION Scenarios for New Relationships Between Japan, Russia, and the United States CO-DIRECTORS Graham Allison Hiroshi Kimura Konstantin Sarkisov Harvard University International Institute for Oriental Research Center Studies for Japanese Studies Belfer Center for Science and International Affairs Harvard Kennedy School 79 JFK Street Cambridge, MA 02138 www.belfercenter.org This report was originally published in 1992 through the Strenghtening Democratic Institutions Project at the then Center for Science and International Affairs. Reproduction Design by Andrew Facini Copyright 2016, President and Fellows of Harvard College Printed in the United States of America BELFER CENTER REPRINT NEW FOREWORD – OCTOBER 2016 Beyond Cold War to Trilateral Cooperation in the Asia-Pacific Region Scenarios for New Relationships Between Japan, Russia, and the United States Co-Directors Graham Allison Hiroshi Kimura Konstantin Sarkisov Harvard University International Institute for Oriental Studies Research Center for Japanese Studies Trilateral Coordinator Fiona Hill Trilateral Task Force Peter Berton, Keith Highet, Pamela Jewett, Masashi Nishihara, Vladimir Yeremin Trilateral Working Group Gelly Batenin, Evgenii Bhanov, Oleg Bondarenko, Timothy Colton, Tsyuoshi Hasegawa, Igor Khan, Alexei Kiva, Alexander Panov, Susan Phar, Sergei Punzhin, Courtney Purrington, Vassily Saplin Foreword October 2016 The first version of this Report on Scenarios for New Relation- ships Between Japan, Russia, and the United States was published 25 years ago. Just after the collapse of the USSR, in anticipation of a visit to Japan by then-Russian President Boris Yeltsin in 1992, we hoped for a breakthrough. Japan and Russia had not signed a formal peace treaty ending World War II for 47 years because of an intractable dispute over the sovereignty of the Northern Territories (to Japanese) or southernmost Kuril Islands (to Russians).
    [Show full text]
  • Online Supplementary Data Cognitive Effects and Acceptability of Non
    BMJ Publishing Group Limited (BMJ) disclaims all liability and responsibility arising from any reliance Supplemental material placed on this supplemental material which has been supplied by the author(s) J Neurol Neurosurg Psychiatry Online supplementary data Cognitive effects and acceptability of non-invasive brain stimulation on Alzheimer’s disease and mild cognitive impairment: a component network meta- analysis Content Page Appendix 1. Detailed search strategy 2 Appendix 2. PRISMA 19 Appendix 3. Flow diagram of trial selection 23 Appendix 4. The characteristics of the included studies 24 Appendix 5. Network plots for the secondary outcomes 31 Appendix 6. Short-term effects on general cognition divided by 32 AD and MCI Appendix 7. Long-lasting effects on general cognition at 1-month 33 follow-up divided by AD and MCI Appendix 8. Forest plots for the executive function 34 Appendix 9. The effect sizes (SMD) for studies with follow-up 35 data longer than 1 month Appendix 10. Adverse events of the included studies 36 Appendix 11. Risk of bias 43 Appendix 12. Inconsistency tests for general cognitive function 44 Appendix 13. Publication bias 45 Appendix 14. Network meta-regression of immediate effect on 46 general cognition Appendix 15. Network meta-regression of 1-month F/U effect on 47 general cognition Appendix 16. Efficacy of sham rTMS vs sham tDCS in general 49 cognitive function 1 Chu C-S, et al. J Neurol Neurosurg Psychiatry 2021; 92:195–203. doi: 10.1136/jnnp-2020-323870 BMJ Publishing Group Limited (BMJ) disclaims all liability and responsibility arising from any reliance Supplemental material placed on this supplemental material which has been supplied by the author(s) J Neurol Neurosurg Psychiatry Appendix 1.
    [Show full text]
  • Finding Aids
    Jurij Lawrynenko Papers BA# ©2007 Columbia University Library SUMMARY INFORMATION Creator Jurij Lawrynenko, 1905-1987 Title and dates Jurij Lawrynenko Papers, 1880s-1980s Abstract The papers are comprised of correspondence, manuscripts, documents, diaries, memoirs, research notes, notebooks, printed material, books, photographs, and audio materials. These papers document the life and work of Jurij Lawrynenko (1905-1987), prominent literature figure of Ukrainian emigration. Size 48 linear feet [66 archival document boxes, 17 archival storage boxes for audio materials, 6 card file boxes, 1 flat box] Call number BA # Location Columbia University Rare Book and Manuscript Library Bakhmeteff Archive Butler Library, 6th Floor Jurij Lawrynenko Papers 535 West 114th Street New York, NY 10027 Languages of material Ukrainian, Russian, English Biographical Note Jurij Adrijanovych Lawrynenko (Dyvnych) born on May 3, 1905, Khyzhyntsi Zvenyhorod district, Kyiv county, was an author, editor, literary theorist and critic, historian, political and social journalist, and prominent figure in the Ukrainian community in the United States. Jurij Lawrynenko graduated from Kharkiv University, where he studied in the Department of Literature and Linguistics from 1926 to 1930. In 1932, he completed his post-graduate education in the Kharkiv Institute on the History of Literature, part of the Ukrainian Academy of Sciences. From 1926 to 1930, he also worked as a manager of the art and proofreading department at the “Visti VUTsVK” newspaper. J. Lawrynenko was arrested twice by the Soviet regime for political offenses and became a prisoner in Stalin’s Gulag system. He was sentenced to five years in a concentration camp followed by three years of exile.
    [Show full text]
  • Dissecting the Heterogeneity in T-Cell Mediated Inflammation In
    cells Review Dissecting the Heterogeneity in T-Cell Mediated Inflammation in IBD Irma Tindemans, Maria E. Joosse and Janneke N. Samsom * Laboratory of Pediatrics, Division Gastroenterology and Nutrition, Erasmus MC-Sophia Children’s Hospital, P.O. Box 2040, 3000 CA Rotterdam, The Netherlands; [email protected] (I.T.); [email protected] (M.E.J.) * Correspondence: [email protected] Received: 30 October 2019; Accepted: 26 December 2019; Published: 2 January 2020 Abstract: Infiltration of the lamina propria by inflammatory CD4+ T-cell populations is a key characteristic of chronic intestinal inflammation. Memory-phenotype CD4+ T-cell frequencies are increased in inflamed intestinal tissue of IBD patients compared to tissue of healthy controls and are associated with disease flares and a more complicated disease course. Therefore, a tightly controlled balance between regulatory and inflammatory CD4+ T-cell populations is crucial to prevent uncontrolled CD4+ T-cell responses and subsequent intestinal tissue damage. While at steady state, T-cells display mainly a regulatory phenotype, increased in Th1, Th2, Th9, Th17, and Th17.1 responses, and reduced Treg and Tr1 responses have all been suggested to play a role in IBD pathophysiology. However, it is highly unlikely that all these responses are altered in each individual patient. With the rapidly expanding plethora of therapeutic options to inhibit inflammatory T-cell responses and stimulate regulatory T-cell responses, a crucial need is emerging for a robust set of immunological assays to predict and monitor therapeutic success at an individual level. Consequently, it is crucial to differentiate dominant inflammatory and regulatory CD4+ T helper responses in patients and relate these to disease course and therapy response.
    [Show full text]