DOCSLIB.ORG

A New Case of Hybrid Epidermoid and Apocrine Cyst

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
A New Case of Hybrid Epidermoid and Apocrine Cyst Case Report A New Case of Hybrid Epidermoid and Apocrine Cyst Fulvia Serra 1,* and Gürkan Kaya 1,2,* 1 Department of Pathology, University Hospital of Geneva, 1205 Geneva, Switzerland 2 Department of Dermatology, University Hospital of Geneva, 1205 Geneva, Switzerland * Correspondence: [email protected] (F.S.); [email protected] (G.K.) Abstract: We described a new case of a hybrid epidermoid and apocrine cyst, known to be a rare histopathological entity. The cyst was located in the axillary region and completely excised, without complication. The diagnosis was made at the histological analysis, where we found a cystic lesion in the dermis, lined with both epidermoid and apocrine epithelium. Keywords: cutaneous cysts; hidrocystoma; hybrid epidermoid; apocrine cyst 1. Introduction Hybrid cutaneous cysts are little known lesions that can be overlooked by the patholo- gist. Inside this small and curious family, the hybrid epidermoid and apocrine cyst is rare and its origin and clinical significance are unknown. Here, we present a new case found in the axillary region in a middle-aged woman with no other known comorbidity. We discuss the histopathological findings, the hypothesis of its origin and we underline the difficulties that can be encountered in reporting this peculiar entity. 2. Case Report A 45-year-old woman with a previous history of two cysts in the right axillary region Citation: Serra, F.; Kaya, G. A New (never excised), admitted to surgically remove one of the two, which measured 0.95 cm. To Case of Hybrid Epidermoid and our knowledge, she does not have any prior disease. The cyst was removed without any Apocrine Cyst. Dermatopathology 2021, complication and sent for analysis with the diagnosis of epidermoid cyst. The healing was 8, 442–445. https://doi.org/10.3390/ uneventful. The histopathological analysis reveal a cystic lesion in the dermis (Figure1). dermatopathology8030046 The cyst is lined in part by a squamous epithelium with an intact granular layer, sometimes atrophic, and in part by a distended apocrine epithelium. The passage between the two Received: 28 June 2021 different epithelia is abrupt. We estimate that the apocrine component represents 30% of Accepted: 19 August 2021 the epithelium. The cyst is filled with keratin lamellae. In proximity of the cyst’s wall, there Published: 1 September 2021 are some apocrine and some sebaceous glands. No further exam was performed. Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affil- iations. Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). Dermatopathology 2021, 8, 442–445. https://doi.org/10.3390/dermatopathology8030046 https://www.mdpi.com/journal/dermatopathology DermatopathologyDermatopathology 20212021,, 88, FOR PEER REVIEW 4432 FigureFigure 1.1. Histology.Histology. ((AA)) HEHE× ×10.10. The wholewhole cyst.cyst. ((BB)) HEHE MagnificationMagnification ××2020 (inset).(inset). Transition betweenbetween epidermoidepidermoid andand apocrine epithelium. (C). HE ×40 Magnification. The apocrine epithelium. apocrine epithelium. (C). HE ×40 Magnification. The apocrine epithelium. 3. Discussion In dermatopathology dermatopathology the the cutaneous cutaneous cysts cysts are are well well known known entities, entities, but have but have been beenclas- classifiedsified in different in different ways ways by bymany many authors. authors. In In the the literature, literature, a a simplified simplified classification classification of cutaneous cysts, according to their origin in the folliculosebaceous u unitnit and sweat glands, is proposed proposed by by Kaya Kaya and and Saurat Saurat [1]. [1 ].With With this this classification classification we wecan can easily easily see that see thatan epi- an epidermaldermal cyst cyst will will have have an aninfundibular infundibular origin, origin, a tricholemmal a tricholemmal cyst cyst an an isthmic isthmic origin, origin, that that a asteatocystoma steatocystoma arise arise from from a a sebaceous sebaceous duct, duct, an eccrine/apocrineeccrine/apocrine hidrocystoma hidrocystoma from thethe glandular epithelial wall, and so on. We findfind this classificationclassification to be a useful tool also to discuss the hybrid cysts: once identified identified the different epithelia, we can easily refer to their origin. A hybrid cyst is in fact simply the combination of different types of epithelia found in the folliculosebaceous unitunit andand sweatsweat glands.glands. TheThe conceptconcept isis introducedintroduced inin aa 19911991 articlearticle on follicular follicular hybrid hybrid cysts, cysts, by by Requena Requena et etal., al., that that defines defines the thehybrid hybrid cyst cystas an as entity an entity aris- arisinging from from one one component component of the of thefolliculosebaceous folliculosebaceous unit unit that that can can combine combine with with others others to toform form a large a large variety variety of follicular of follicular hybrid hybrid cysts, cysts, that that show show a distinctive a distinctive two two-component-component dif- differentiation,ferentiation, with with an an abrupt abrupt transition transition between between them them [2]. [2]. It It is is in in this this peculiar peculiar family thatthat we findfind thethe hybridhybrid epidermoid epidermoid and and apocrine apocrine cyst, cyst, a combinationa combination of epidermoidof epidermoid epithelium epithe- withlium awith hidrocystoma. a hidrocystoma. The epidermoid cyst is a well-knownwell-known and common entity that is usually found on the face, neck, trunk and perinealperineal area. The cyst is linedlined byby aa squamoussquamous cellcell epitheliumepithelium thatthat can be normal, atrophicatrophic oror hyperplastic,hyperplastic, but but always always with with a a well-defined well-defined and and intact intact granular granu- layer.lar layer. It is It filled is filled with with keratin keratin lamellae. lamellae. The apocrine hidrocystoma is uncommon, it is rarely found in the axillary region (it usuallyusually arisesarises inin the the head head and and neck neck area) area) and and is generallyis generally a standalone a standalone lesion. lesion. Histologically, Histologi- itcally, appears it appears as a cystic as a cystic lesion, lesion, lined lined by a by double a double layer layer of epithelial of epithelial cells, cells, the the outer outer layer layer of flattenedof flattened myoepithelial myoepithelial cells cells and and the the inner inner layer layer of cuboidof cuboid columnar columnar cells, cells, with with round round or ovalor oval nuclei nuclei and and decapitation decapitation secretion secretion usually usually present. present. Cyst Cyst distention distention can cause can cause flattening flat- andtening atrophy and atrophy of the epithelium, of the epithelium, therefore therefore sometimes sometimes it can be it difficult can be todifficult distinguish to distinguish between anbetween apocrine an apocrine and an eccrine and an hidrocystoma.eccrine hidrocystoma. Since the Since apocrine the apocrine and eccrine and eccrine origins origins come fromcome twofrom ductal two ductal systems systems that are that thought are thought to be identical, to be identical, some authors some haveauthors proposed have pro- the alternativeposed the alternative term of “ductal term of hidrocystoma”, “ductal hidrocystoma”, to reflect the to reflect possible the dual possible histogenesis dual histogen- [3]. esis [To3]. our knowledge, there are only few documented cases of hybrid epidermoid and apocrineTo our cyst knowledge, [4,5]. In a casethere report, are only Andersen few docum et al.,ented describe cases fourof hybrid cysts inepidermoid four different and patients:apocrine cyst one [4,5 on the]. In eyelid,a case report, one on Andersen the lip and et twoal., describe on the nipple,four cysts measured in four betweendifferent 0.5patients: and 0.6one cm on [the4]. eyelid, The histopathological one on the lip and analysis two on shows the nipple, the cysts measured lined withbetween foci 0.5 of Dermatopathology 2021, 8 444 cuboidal eosinophilic cells and decapitation secretion, alternated with a stratified squamous epithelium with an intact granular layer immediately adjacent. The luminas are filled with keratin lamellae. Regarding the location, in a previous case report, Bourlond et al. [6] described two cases of erosive adenomatosis of the nipple, suggesting that an apocrine differentiation in a nipple’s cyst is not that uncommon as thought to be. Despite this argument, the more common location of the hybrid cysts with follicular and apocrine differentiation seems to for now to be the eyelid, like can sometimes happen in Moll gland cyst [3] and as described by Milman et al. [5]. It is noteworthy that our case is the first hybrid epidermoid and apocrine cyst to be found in the axillary region. We do not find this to be a surprising location for such a lesion, being aware of the abundance of apocrine glands in this area. That said, we have to consider that maybe the hybrid epidermoid and apocrine cyst is underdiagnosed: especially when the apocrine component is present in less than 50% of the epithelium, it can be missed depending on the embedding and cutting level. As for the pathogenesis, it is still unknown. It is possible that this peculiar entity arises from
Load more

Recommended publications
  • An Analysis of Benign Human Prostate Offers Insights Into the Mechanism
    www.nature.com/scientificreports OPEN An analysis of benign human prostate ofers insights into the mechanism of apocrine secretion Received: 12 March 2018 Accepted: 22 February 2019 and the origin of prostasomes Published: xx xx xxxx Nigel J. Fullwood 1, Alan J. Lawlor2, Pierre L. Martin-Hirsch3, Shyam S. Matanhelia3 & Francis L. Martin 4 The structure and function of normal human prostate is still not fully understood. Herein, we concentrate on the diferent cell types present in normal prostate, describing some previously unreported types and provide evidence that prostasomes are primarily produced by apocrine secretion. Patients (n = 10) undergoing TURP were prospectively consented based on their having a low risk of harbouring CaP. Scanning electron microscopy and transmission electron microscopy was used to characterise cell types and modes of secretion. Zinc levels were determined using Inductively Coupled Plasma Mass Spectrometry. Although merocrine secretory cells were noted, the majority of secretory cells appear to be apocrine; for the frst time, we clearly show high-resolution images of the stages of aposome secretion in human prostate. We also report a previously undescribed type of epithelial cell and the frst ultrastructural image of wrapping cells in human prostate stroma. The zinc levels in the tissues examined were uniformly high and X-ray microanalysis detected zinc in merocrine cells but not in prostasomes. We conclude that a signifcant proportion of prostasomes, possibly the majority, are generated via apocrine secretion. This fnding provides an explanation as to why so many large proteins, without a signal peptide sequence, are present in the prostatic fuid. Tere are many complications associated with the prostate from middle age onwards, including benign prostatic hyperplasia (BPH) and prostate cancer (PCa).
    [Show full text]
  • Epithelial Tissue
    Epithelial Tissue Epithelial Tissue Tissues - Introduction · a group of similar cells specialized to carry on a particular function · tissue = cells + extracellular matrix nonliving portion of a tissue that supports cells · 4 types epithelial - protection, secretion, absorption connective - support soft body parts and bind structures together muscle - movement nervous - conducts impulses used to help control and coordinate body activities Epithelial Tissues Characteristics Epithelial Classifications · free surface open to the outside or an open · classified based on shape and # of cell layers internal space (apical surface) · shape · basement membrane anchors epithelium to squamous - thin, flat cells underlying connective tissue cuboidal - cube-shaped cells columnar - tall, elongated cells · lack blood vessels · number · readily divide (ex. skin healing) simple - single layer · tightly packed with little extracellular space stratified - 2 or more layers Epithelial Locations Simple Squamous Epithelium · a single layer of thin, flattened cells · cover body surfaces, cover and line internal organs, and compose glands looks like a fried egg · easily damaged skin cells, cells that line the stomach and small intestine, inside your mouth · common at sites of filtration, diffusion, osmosis; cover surfaces · air sacs of the lungs, walls of capillaries, linings cheek cells of blood and lymph vessels intestines skin Epithelial Tissue Simple Cuboidal Epithelium Simple Columnar Epithelium · single layer of cube-shaped cells · single layer of cells
    [Show full text]
  • A Focus on Breast Cancer
    HHS Public Access Author manuscript Author ManuscriptAuthor Manuscript Author Pharmacol Manuscript Author Ther. Author Manuscript Author manuscript; available in PMC 2017 May 01. Published in final edited form as: Pharmacol Ther. 2016 May ; 161: 79–96. doi:10.1016/j.pharmthera.2016.03.003. Emerging therapeutic targets in metastatic progression: a focus on breast cancer Zhuo Li and Yibin Kang* Department of Molecular Biology, Princeton University, Princeton, NJ, 08544, United States Abstract Metastasis is the underlying cause of death for the majority of breast cancer patients. Despite significant advances in recent years in basic research and clinical development, therapies that specifically target metastatic breast cancer remain inadequate, and represents the single greatest obstacle to reducing mortality of late-stage breast cancer. Recent efforts have leveraged genomic analysis of breast cancer and molecular dissection of tumor-stromal cross-talk to uncover a number of promising candidates for targeted treatment of metastatic breast cancer. Rational combinations of therapeutic agents targeting tumor-intrinsic properties and microenvironmental components provide a promising strategy to develop precision treatments with higher specificity and less toxicity. In this review, we discuss the emerging therapeutic targets in breast cancer metastasis, from tumor-intrinsic pathways to those that involve the host tissue components, including the immune system. Keywords Breast cancer; Metastasis; Targeted therapy; Tumor microenvironment; Immunotherapy 1. Introduction The overall 5-year survival rate for breast cancer currently stands at 90% — a dramatic improvement over the 63% survival rate in the early 1960s. When stratified by stage, the 5- year survival rates have increased to 99% for localized disease and 85% for regional advanced disease, a trend that can be attributed to early diagnoses and better treatment regimens.
    [Show full text]
  • A New Look at Transudation: the Apocrine Connection
    Physiol. Res. 69: 227-244, 2020 https://doi.org/10.33549/physiolres.934229 REVIEW A New Look at Transudation: The Apocrine Connection Robert FARKAŠ1, Milan BEŇO1, Denisa BEŇOVÁ-LISZEKOVÁ1, Ivan RAŠKA2, Otakar RAŠKA2,3 1Laboratory of Developmental Genetics, Institute of Experimental Endocrinology, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovak Republic, 2Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University, Prague, Czech Republic, 3Institute of Pathophysiology, Third Faculty of Medicine, Charles University, Prague, Czech Republic Received June 9, 2019 Accepted December 20, 2019 Epub Ahead of Print March 23, 2020 Summary Corresponding authors Transcellular trafficking in which various molecules are Robert Farkaš, Laboratory of Developmental Genetics, Institute transported across the interior of a cell, is commonly classified as of Experimental Endocrinology, Biomedical Research Center, transcytosis. However, historically this term has been used Slovak Academy of Sciences, Dúbravská cesta 9, 84505 synonymously with transudation. In both cases transcellular Bratislava, Slovak Republic. E-mail: [email protected]. Otakar trafficking starts with the internalization of proteins or other Raška, Third Faculty of Medicine, Charles University, Ruská 87, compounds on the basal or basolateral side of a cell and 10000 Prague, Czech Republic. E-mail: [email protected] continues by their transport across the interior to the apical pole (or vice versa) where they are subsequently released.
    [Show full text]
  • Volume 21 • 2015
    Weill Co rnell Medical College Volume 21 • January 2015 The Newsletter of the Department of Pathology and Laboratory Medicine at NewYork-Presbyterian Hospital/Weill Cornell Medical Center Contents Research Highlights Localization of Annexin A5 on the surface of placental trophoblasts 1 by David P. Hajjar, PhD Research Highlights 2 Focus (green: AnxA5; blue: nucleus) Figure 1 : Expression of AnxA5 (green fluorescence) on 3 cultured syncytialized human placental trophoblasts. Clinical Pathology Update Blue (DAPI) fluorescence marks the nuclei. [bars = 50 ␮m. Confocal microscopy 3-dimensional projections of Z-axis image stacks, voxel size ( ␮m): width 0.73, height 0.73, and 4-6 Drs. Jacob Rand and Xiao-Xuan Wu. depth 1.50] (for complete description, see Wu XX, Guller S, The Department is delighted to welcome Dr. Jacob Rand , Rand JH. Hydroxychloroquine reduces binding of antiphos - Keynotes pholipid antibodies to syncytiotrophoblasts and restores Professor of Pathology and Laboratory Medicine, as Vice annexin A5 expression. Am J Obstet Gynecol . 2011 Dec; Chairman for Laboratory Medicine and Director of the Clinical 205(6):576.e7-14.) 7 Laboratories. Pathology Faculty’s Dr. Rand comes to us from Montefiore Medical Center and Global Travels the Albert Einstein College of Medicine where he served as Atomic force Microscopy: Structure of the Director of Hematology, Advanced Coagulation and Protein aPL Ig- ␤2GPI Complexes Separation Laboratories and as Professor of Pathology, 8 Medicine, and Obstetrics and Gynecology, and Director of the -HCQ Resident’s Corner Hematology Laboratories. Dr. Rand’s record of achievement spans across disciplines, with strong backgrounds as a clini cal physician, clinical laboratory director, teacher and 9-11 +HCQ basic scientist.
    [Show full text]
  • (Eccrine and Apocrine Glands) During Childhood in Japanese, Especially on the PAS Positive Substance and Iron
    Histochemical Investigation on the Axillary Sweat Glands (Eccrine and Apocrine Glands) during Childhood in Japanese, especially on the PAS Positive Substance and Iron By Iwao Yasui and Hiroshi Kagemoto Department of Anatomy, School of Medicine, Keio University Shinjuku, Tokyo, Japan (Director : Prof. Dr. T. Taniguchi) Introduction The application of periodic acid to histochemical study on poly- saccharide was made for the first time by Hot c h k i s s, M c Man u s, Lillie et G r e co and Marches e. This histochemical method was called for short PAS method " (namely, Periodic Acid S c h i f f). 'Recently , a good deal of the histochemical studies regarding glyco- gen, mucopolysaccharide and other substances in the sweat glands have been made by such various scholars as Bunt in g, Wislocki and Dempsey (1948), Montagna, Chase and Lobitz (1952), Montagna, Chase and M e l a r a g n o (1951) and others, apply- ing the PAS method. On the other hand, studies on the iron reaction in the sweat glands have been numerously reported since H o m m a (1925)'s research on the axillary sweat glands by Turnbull reaction, that is, Klaar (1926), Herzenberg (1927), Moriyama (1927), Oono and Kinoshita (1927), Wosllard (1930), Richter (1933), Manca (1934), Way and Memmesheimer (1938), Yoshi- mura (1942), Nagamitsu (1941), Yoshihiro (1942a), Cavaz- zana (1947), Bunting, Wislocki and Dempsey (1948),Iwa- shige (1951),Montagna,Chase and Lobitz (1953) and others. Nevertheless, there have been no reports which give histochemical observation on the axillary sweat glands during childhood by age group.
    [Show full text]
  • Frequency Vs. Interval
    Frequency vs. Interval • Milking Frequency – 2 times/day (2X) or 3 times/day (3X) • Milking Interval – 12 hours, 8 hours, etc. • How often do cows want to be milked? Frequency vs. Interval What are the advantages and disadvantages of more frequent milkings? Chemotactic agents: attract PMN into tissues & milk! • Alveoli • Basic milk-producing unit • Lined with epithelial cells • Phagocyte • Cell that engulfs and absorbs bacteria • PMN • Polymorphonuclear neutrophil • First line of defense against invading pathogens during mastitis • Majority cell type accounting for SCC • Macrophages, lymphocytes • Chemotaxis • Movement of an organism in response to a chemical stimulus • Somatic cells and bacteria move according to chemicals in their environment • Where and why would they be moving? • What is a common example of chemotaxis unrelated to milk secretion? Altered Composition During Mastitis Somatic cell counts (SCC) Na, Cl, whey protein (e.g., serum albumin, Ig) lactose, casein, K, α-lactalbumin Altered Composition During Mastitis • Lactose • Synthesis is decreased • Casein • Proteolysis • Proteolytic enzymes from leukocytes and bacteria • Milk fat • Susceptibility of milk fat globule membranes to the action of lipases, resulting in breakdown of triglycerides. Altered Composition During Mastitis • Na+, Cl-, K+ • Electrical potential across apical membrane disrupted • This is the basis of the electrical conductivity methods of detecting mastitis • https://www.youtube.com/watch?v=P-imDC1txWw • Polymorphonuclear neutrophils (PMNs) • Mastitis causes chemotaxis of the cells into the tissue and disruption of epithelial tight junctions • This is the basis of many mastitis detection methods • Albumin, immunoglobulins • Enter the milk via disrupted tight junctional complexes PHYLOGENY & ONTOGENY Phylogeny – the evolutionary development of any animal species (related to mammary gland development) Class Mammalia: Monotremes I.
    [Show full text]
  • 2018 Solid Tumor Rules Lois Dickie, CTR, Carol Johnson, BS, CTR (Retired), Suzanne Adams, BS, CTR, Serban Negoita, MD, Phd
    Solid Tumor Rules Effective with Cases Diagnosed 1/1/2018 and Forward Updated November 2020 Editors: Lois Dickie, CTR, NCI SEER Carol Hahn Johnson, BS, CTR (Retired), Consultant Suzanne Adams, BS, CTR (IMS, Inc.) Serban Negoita, MD, PhD, CTR, NCI SEER Suggested citation: Dickie, L., Johnson, CH., Adams, S., Negoita, S. (November 2020). Solid Tumor Rules. National Cancer Institute, Rockville, MD 20850. Solid Tumor Rules 2018 Preface (Excludes lymphoma and leukemia M9590 – M9992) In Appreciation NCI SEER gratefully acknowledges the dedicated work of Dr. Charles Platz who has been with the project since the inception of the 2007 Multiple Primary and Histology Coding Rules. We appreciate the support he continues to provide for the Solid Tumor Rules. The quality of the Solid Tumor Rules directly relates to his commitment. NCI SEER would also like to acknowledge the Solid Tumor Work Group who provided input on the manual. Their contributions are greatly appreciated. Peggy Adamo, NCI SEER Elizabeth Ramirez, New Mexico/SEER Theresa Anderson, Canada Monika Rivera, New York Mari Carlos, USC/SEER Jennifer Ruhl, NCI SEER Louanne Currence, Missouri Nancy Santos, Connecticut/SEER Frances Ross, Kentucky/SEER Kacey Wigren, Utah/SEER Raymundo Elido, Hawaii/SEER Carolyn Callaghan, Seattle/SEER Jim Hofferkamp, NAACCR Shawky Matta, California/SEER Meichin Hsieh, Louisiana/SEER Mignon Dryden, California/SEER Carol Kruchko, CBTRUS Linda O’Brien, Alaska/SEER Bobbi Matt, Iowa/SEER Mary Brandt, California/SEER Pamela Moats, West Virginia Sarah Manson, CDC Patrick Nicolin, Detroit/SEER Lynda Douglas, CDC Cathy Phillips, Connecticut/SEER Angela Martin, NAACCR Solid Tumor Rules 2 Updated November 2020 Solid Tumor Rules 2018 Preface (Excludes lymphoma and leukemia M9590 – M9992) The 2018 Solid Tumor Rules Lois Dickie, CTR, Carol Johnson, BS, CTR (Retired), Suzanne Adams, BS, CTR, Serban Negoita, MD, PhD Preface The 2007 Multiple Primary and Histology (MPH) Coding Rules have been revised and are now referred to as 2018 Solid Tumor Rules.
    [Show full text]
  • Review Apocrine Secretory Mechanism
    Histol Histopathol (2003) 18: 597-608 Histology and http://www.hh.um.es Histopathology Cellular and Molecular Biology Review Apocrine secretory mechanism: Recent findings and unresolved problems A.P. Gesase1 and Y. Satoh2 1Department of Anatomy/Histology, Muhimbili University College of Health Sciences, Dar es salaam, Tanzania and 2Department of Histology, School of Medicine, Iwate Medical University, Morioka, Japan Summary. Cell secretion is an important physiological Introduction process that ensures smooth metabolic activities, tissue repair and growth and immunological functions in the Apocrine secretion occurs when secretory process is body. It occurs when the intracellular secretory materials accompanied with loss of part of the cell cytoplasm (Fig. are released to the exterior; these may be in the form of 1). The secretory materials may be contained in the lipids, protein or mucous and may travel through a duct secretory vesicles or dissolved in the cytoplasm and system or via blood to reach the target organ. To date during secretion they are released as cytoplasmic three types of secretory mechanisms have been fragments into the glandular lumen or interstitial space characterized, they include apocrine, holocrine and (Roy et al., 1978; Agnew et al., 1980; Ream and exocytosis. Apocrine secretion occurs when the release Principato, 1981; Messelt, 1982; Eggli et al., 1991; of secretory materials is accompanied with loss of part Gesase et al. 1996). It has been described in glands of of cytoplasm. The secretory materials may be contained the genital tract (Nicander et al., 1974; Aumuller and in the secretory vesicles or dissolved in the cytoplasm Adler, 1979; Guggenheim et al., 1979; Hohbach and that is lost during secretion.
    [Show full text]
  • Genitourinary PAX8
    174A ANNUAL MEETING ABSTRACTS RMC and 19/21 (90%) of CDC cases. In contrast, 31/34 (91%) UUC were negative for Genitourinary PAX8. p63: p63 was positive in 7/12 (58%) RMC and in 3/21 (14%) CDC. Staining was focal in 6/7 RMC and strong in 4/7. Almost all (97%) UUC were p63 positive 767 Histopathologic Features of Bilateral Renal Cell Carcinomas: A (moderate/strong and multifocal/diffuse in 80% of cases). The one p63 negative UUC Study of 24 Cases was a microinvasive high grade tumor and was also negative for PAX8. J Abdelsayed, JY Ro, LD Truong, AG Ayala, SS Shen. The Methodist Hospital and Weill Conclusions: We suggest a binary panel of PAX8 and p63 as an aid in the differential Medical College of Cornell University, Houston, TX. diagnosis of high grade renal sinus epithelial neoplasms. (PAX8+/p63+) profile Background: The incidence of bilateral renal cell carcinoma (bRCC) has been reported supported the dx of RMC with a sensitivity of 58.3% and specificity of 89%. (PAX8+/ to vary from 1.5% to 11%. Clear understanding of the clinicopathologic features of p63-) profile supported the diagnosis of CDC with a sensitivity of 85.7% and a specificity bRCCs including the distinction between synchronous and metachronous tumors has of 89%. Finally (PAX8-/p63+) profile supported the diagnosis of UUC with a sensitivity important implications in patients’ management and follow up. The purpose of this study of 88% and a specificity of 100%. The concomitant expression of p63 and PAX8 in RMC is to summarize the clinicopathologic features of bRCCs and compare them with those seen in our study further suggests an intermediate phenotype between renal tubular and of unilateral renal cell carcinomas (uRCCs).
    [Show full text]
  • Human Sweat Gland Myoepithelial Cells Express a Unique Set Of
    Journal of Cell Science 112, 1925-1936 (1999) 1925 Printed in Great Britain © The Company of Biologists Limited 1999 JCS4638 Human sweat gland myoepithelial cells express a unique set of cytokeratins and reveal the potential for alternative epithelial and mesenchymal differentiation states in culture Margarete Schön1,*, Jennifer Benwood1, Therese O’Connell-Willstaedt2 and James G. Rheinwald1,2,‡ 1Division of Dermatology/Department of Medicine, Brigham and Women’s Hospital, and 2Division of Cell Growth and Regulation, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA *Present address: Department of Dermatology, Heinrich-Heine University, Moorenstrasse 5, 40225 Düsseldorf, Germany ‡Author for correspondence (e-mail: [email protected]) Accepted 9 April; published on WWW 26 May 1999 SUMMARY We have characterized precisely the cytokeratin expression myoepithelial cells, a constituent of secretory glands. pattern of sweat gland myoepithelial cells and have Immunostaining of skin sections revealed that only sweat identified conditions for propagating this cell type and gland myoepithelial cells expressed the same pattern of modulating its differentiation in culture. Rare, unstratified keratins and α-sma and lack of E-cadherin as the cell type epithelioid colonies were identified in cultures initiated we had cultured. Interestingly, our immunocytochemical from several specimens of full-thickness human skin. These analysis of ndk, a skin-derived cell line of uncertain cells divided rapidly in medium containing serum, identity, suggests that this line is of myoepithelial origin. epidermal growth factor (EGF), and hydrocortisone, and Earlier immunohistochemical studies by others had found maintained a closely packed, epithelioid morphology when myoepithelial cells to be K7-negative.
    [Show full text]
  • Apocrine Carcinoma of the Breast: a Comprehensive Review
    See discussions, stats, and author profiles for this publication at: https://www.researchgate.net/publication/239075330 Apocrine carcinoma of the breast: A comprehensive review Article in Histology and histopathology · June 2013 Source: PubMed CITATIONS READS 12 1,947 7 authors, including: Semir Vranic Anna Sapino University of Sarajevo Università degli Studi di Torino 101 PUBLICATIONS 451 CITATIONS 359 PUBLICATIONS 6,132 CITATIONS SEE PROFILE SEE PROFILE Jose L Costa Zoran Gatalica University of Porto Caris Life Sciences 57 PUBLICATIONS 765 CITATIONS 310 PUBLICATIONS 4,467 CITATIONS SEE PROFILE SEE PROFILE All in-text references underlined in blue are linked to publications on ResearchGate, Available from: Semir Vranic letting you access and read them immediately. Retrieved on: 22 August 2016 Histol Histopathol (2013) 28: 1393-1409 Histology and http://www.hh.um.es Histopathology Cellular and Molecular Biology Review Apocrine carcinoma of the breast: A comprehensive review Semir Vranic1,2, Fernando Schmitt3,4, Anna Sapino2, Jose Luis Costa4, Sandeep Reddy5, Michael Castro5 and Zoran Gatalica5 1Department of Pathology, Clinical Center of the University of Sarajevo, Sarajevo, Bosnia and Herzegovina, 2Department of Medical Sciences, University of Turin, Turin, Italy, 3Department of Pathology and Oncology, Medical Faculty of Porto University, Porto, Portugal, 4Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal and 5Caris Life Sciences, Phoenix, Arizona, United States of America Summary. Apocrine carcinoma of the breast is a rare, Definition special type of breast carcinoma showing distinct morphologic, immunohistochemical and molecular Mammary apocrine cells have abundant eosinophilic genetic features. Apocrine epithelium has a and granular cytoplasm (“type A” cells), centrally to characteristic steroid receptor profile that is estrogen eccentrically located nuclei with prominent nucleoli and receptor and progesterone receptor negative and distinctive cell borders (Eusebi et al., 1986; Tavassoli, androgen receptor positive.
    [Show full text]