Oxidative Medicine and Cellular Longevity – Special Issue: Cardiolipin and Mitochondria-Targeted Antioxidants in Oxidative Stress, Disease, and Aging Prediabetes induced by fructose-enriched diet influences cardiac lipidome and proteome and leads to deterioration of cardiac function prior to the development of excessive oxidative stress and cell damage 1,2Gergő Szűcs*†, 1,2Andrea Sója†, 3Mária Péter†, 1,2Márta Sárközy, 2,4Bella Bruszel, 5Andrea Siska, 5Imre Földesi, 2,4Zoltán Szabó, 2,4Tamás Janáky, 3László Vígh, 3Gábor Balogh#, 1,2Tamás Csont#,* 1Metabolic Diseases and Cell Signaling Group, Department of Biochemistry, Faculty of Medicine, University of Szeged, Szeged H-6720, Hungary 2Interdisciplinary Centre of Excellence, University of Szeged, Szeged H-6720, Hungary 3Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, Szeged H-6726, Hungary 4Institute of Medical Chemistry, Faculty of Medicine, University of Szeged, Szeged H-6720, Hungary. 5Department of Laboratory Medicine, Faculty of Medicine, University of Szeged, Szeged H-6720, Hungary Working Title: Cardiolipin in prediabetic cardiac dysfunction. *Correspondence Gergő Szűcs;
[email protected] Tamás Csont;
[email protected] †Equal contributors #Equal senior contributors Keywords: fructose diet, diastolic dysfunction, cardiolipin, lipidome, proteome, oxidative stress, apoptosis, adaptation Supplementary Methods Lipidomics Lipidomic analyses were performed on an LTQ-Orbitrap Elite instrument (Thermo Fisher Scientific) equipped with a robotic nanoflow ion source TriVersa NanoMate (Advion BioSciences) using chips with the diameter of 5.5-μm spraying nozzles. The ion source was controlled by Chipsoft 8.3.1 software. The ionization voltages were +1.3 kV and −1.9 kV in posive and negave mode, respectively, and the back-pressure was set at 1 psi in both modes.