The Impact of Silicon Fertilisation on the Chemical Ecology of Grapevine
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The impact of silicon fertilisation on the chemical ecology of grapevine, Vitis vinifera; constitutive and induced chemical defences against arthropod pests and their natural enemies Vanessa J. Connick A thesis submitted in fulfilment of the requirements for the degree of Master of Philosophy Faculty of Science School of Agriculture and Wine Sciences July 2011 1 Table of Contents Certificate of Authorship ............................................................................. 7 Certificate of Authorship ............................................................................. 7 Acknowledgements ....................................................................................... 8 Editorial Note ............................................................................................. 11 Publications associated with this thesis .................................................... 12 Abbreviations used in this thesis ............................................................... 13 Abstract ....................................................................................................... 14 Chapter 1 Literature Review .................................................................. 21 1.1 Introduction ................................................................................... 22 1.2 Chemical ecology of silicon–plant interactions ............................ 26 1.2.1 Silicon and soil ......................................................................... 26 1.2.2 Silicon and water ...................................................................... 28 1.2.3 Silicon and plants ...................................................................... 28 1.2.4 Silicon uptake by grapevines .................................................... 35 1.3 Role of silicon in plant defences ................................................... 36 1.3.1 Role of silicon in constitutive plant defences ........................ 37 1.3.2 Role of silicon in induced plant defences .............................. 38 1.4 Herbivore-induced plant volatiles (HIPVs) ................................... 42 1.4.1 Plant volatiles and herbivore-induced plant volatiles ............... 42 1.5 Metabolic pathways that drive induced plant defences ................. 47 1.5.1 Isoprenoids (terpenoids) ......................................................... 47 1.5.2 Oxylipins ................................................................................ 49 1.5.3 Volatile aromatic compounds ................................................ 49 1.5.4 Interactions between the metabolic pathways ........................ 50 1.6 Use of herbivore-induced plant volatiles as an integrated pest management strategy ................................................................................ 51 1.7 Link between silicon and induced chemical defences ................... 53 1.8 Specific benefits of silicon fertilisation to grapevines .................. 55 1.9 Agricultural and commercial uses for silicon ................................ 57 1.9.1 Forms of silicon ........................................................................ 57 1.9.2 Soil versus foliar application of silicon .................................... 58 1.9.3 Adverse effects of silicon fertilisation on beneficial arthropods ............................................................................................................ 60 1.10 Grapevine pests of Australia and their natural enemies ............ 60 1.10.1 Arthropod pests ....................................................................... 61 1.10.2 Beneficial arthropods (predators and parasitoids) .................. 64 1.11 Aims and scope of this research study .......................................... 67 Chapter 2 Effects of silicon fertilisation of grapevines on feeding damage of the pest, light brown apple moth, Epiphyas postvittana (Walker) (Lepidoptera: Tortricidae)........................................................ 69 2.1 Introduction ................................................................................... 70 2.2 Materials and methods ................................................................... 75 2.2.1 Site ......................................................................................... 75 2.2.2 Plant culture ........................................................................... 75 2.2.3 Insect culture .......................................................................... 77 2.2.4 Treatments .............................................................................. 78 2.2.5 Experimental design ............................................................... 78 2.2.6 Statistical analysis .................................................................. 79 2.3 Results ........................................................................................... 80 3 2.3.1 Area of leaf consumed ........................................................... 80 2.3.2 Larval weight gain/loss .......................................................... 81 2.3.3 Relationship between leaf area consumed and larval weight . 81 2.4 Discussion ..................................................................................... 83 Chapter 3 Silicon enhances induced plant defence, attracting the predatory beetle, Dicranolaius bellulus (Guérin-Méneville) (Coleoptera: Melyridae) to grapevine plants infested with Epiphyas postvittana (Walker) (Lepidoptera: Tortricidae) larvae ............................................ 89 3.1 Introduction ................................................................................... 90 3.2 Materials and methods ................................................................... 92 3.2.1 Insect culture .......................................................................... 92 3.2.2 Treatment ............................................................................... 92 3.2.3 Experimental Design ............................................................... 93 3.2.4 Plant and potting medium analysis ........................................ 95 3.2.5 Statistical analysis .................................................................. 96 3.3 Results ........................................................................................... 96 3.4 Discussion ..................................................................................... 98 Chapter 4 The effects of silicon fertilisation on the induced defences of grapevine to grapevine moth, Phalaenoides glycinae (Lewin) (Lepidoptera: Noctuidae): HIPV emission and attraction to the predatory green lacewing, Mallada signata (Schneider) (Neuroptera: Crysopidae) ............................................................................................... 102 4.1 Introduction ................................................................................. 103 4.2 Materials and methods ................................................................. 106 4.2.1 Site ....................................................................................... 106 4.2.2 Plant culture ......................................................................... 107 4 4.2.3 Insect cultures....................................................................... 109 4.2.4 Treatment ............................................................................. 109 4.2.5 Experimental design ............................................................. 110 4.2.6 Statistical analysis ................................................................ 114 4.3 Results ......................................................................................... 115 4.3.1 Climate conditions experienced in glasshouse ................. 115 4.3.2 Si content in deionised water, potting medium and plant tissue 115 4.3.3 SPME/GC-MS analysis of volatiles ..................................... 115 4.3.4 Olfactometer bioassays ........................................................ 117 4.4 Discussion ................................................................................... 118 Chapter 5 Response of the green lacewing, Mallada signata (Schneider) (Neuroptera: Chrysopidae) to synthetic herbivore-induced plant volatiles ............................................................................................ 124 5.1 Introduction ................................................................................. 125 5.2 Materials and methods ................................................................. 129 5.2.1 Experimental design ............................................................. 129 5.2.2 Statistical analysis ................................................................ 131 5.3 Results ......................................................................................... 131 5.4 Discussion ................................................................................... 132 Chapter 6 General Discussion ............................................................ 139 6.1 Direct effects of silicon fertilisation of grapevines on the pest, Epiphyas postvittana .............................................................................. 141 6.2 Tri-trophic effects of silicon fertilisation of grapevines on the beneficial insects, Dicranolaius bellulus and Mallada signata ............. 142 5 6.3 Response of Mallada signata to HIPVs ...................................... 144 6.4 Silicon content in grapevine leaf tissue ....................................... 147 6.5 Plant-available silicon content in