Supplementary Table 1. Primer sequences

Target gene Sequence qPCR GAPDH Forward 5’-CTCTCTGCTCCTCCTGTTCGAC-3’ Reverse 5’-TGAGCGATGTGGCTCGGCT -3’ RNF43 Forward 5’-GCCACCGGCACCACCACTAC -3’ Reverse 5’-GGGTTAGAGAGCCGCCCCGA -3’ LAMC1 Forward 5’-TGGAGGAGGGCAACTGCGGA-3’ Reverse 5’-ACCTCTCACAGTGGGCGCCA-3’ EIF2S1 Forward 5’- GGTGGAGTGAGCGAAGCGCA-3’ Reverse 5’-ACCCGGCATTCTGAGGTATGTGTG -3’ PPP2R1B Forward 5’-GCCCACTGTCTGCTGCCTCC -3’ Reverse 5’-TGCGAGAGGTGAACCAATCCCCA -3’ ITGB4 Forward 5’-GCCCAGAGCGGGGAGGACTAC -3’ Reverse 5’-TTCCAGCCGCAGCCAGTGTCAT -3’ IL24 Forward 5’-GTGGACTTTAGCCAGCAAGCTCAGG -3’ Reverse 5’-CCGCCTGTGTGCACTGTCTCTG -3’ CDK2 Forward 5’-TCTTTGCTGAGATGGTGACTCGCC -3’ Reverse 5’-CGGGCCCACTTGGGGAAACTTG -3’ CCNE2 Forward 5’- GCTGGTCTGGCGAGGTTTTATACAC-3’ Reverse 5’- TGGCCTGGATTATCTGGGCTTCT-3’ CDKN1C Forward 5’-AACGGCGCGGCGATCAAGAA -3’ Reverse 5’-GGGCTCTAAATTGGCTCACCGCA -3’ FABP3 Forward 5’-TCATCCTGACACTCACCCACGG -3’ Reverse 5’-GCTGAGTCGAGGGGTAGCCGA -3’ CDC23 Forward 5’-AGCAAATGGTCGGCGGAGTTGG -3’ Reverse 5’-TGCAGGAAATGTGCTGCCCGA -3’ TIMP3 Forward 5’- GCGGCAATGACCCCTTGGCT -3’ Reverse 5’- GGCCCGGATCACGATGTCGG -3’ LIFR Forward 5’- CGCGGGAGCGTACCGACTGA-3’ Reverse 5’-ATGAGGAGCCCCCTTTTTCTGGCT -3’ ERBB3 Forward 5’- CCGCTTGACTCAGCTCACCGA-3’ Reverse 5’-GGGGGACAGCTTCTGCCATTGT -3’ ARHGAP5 Forward 5’-TGCTGTAGCTGGAGCCCTTAAAGC -3’ Reverse 5’-TCCGGGATTTTTGCTGCTTCCA -3’ RAD23A Forward 5’- TCCACGCTAGTGACGGGCTCT -3’ Reverse 5’- GGCTCGGTGGGGGTTGTTGT -3’ IL1R1 Forward 5’- TGGGGAAGGGTCTACCTCTGACTGT -3’ Reverse 5’- ACCTCAACAATGTCTTCCCCAACGT -3’ IL6R Forward 5’- GCGACAAGCCTCCCAGTGCAA -3’ Reverse 5’- ATGGCAATGCAGAGGAGCGTTCC -3’ PRKCA Forward 5’- GCGTCTCAGGACGTGGCCAAC -3’ Reverse 5’- TTTCCCAAACCCCCAGATGAAGTCG-3’ CASP7 Forward 5’- CCGTGGGAACGATGGCAGATGAT -3’ Reverse 5’- CTCGGTCCCGGGTGGTCTTGAT-3’ BAX Forward 5’- GCGCATCGGGGACGAACTGG -3’ Reverse 5’- GCACAGGGCCTTGAGCACCA -3’ SPP1 Forward 5’- GGCATCACCTGTGCCATACCAGT -3’ Reverse 5’- TGGAAGGGTCTGTGGGGCTAGGA -3’ INHBA Forward 5’- CGGCGCTTCTGAACGCGATC -3’ Reverse 5’- GCTGTTCCTGACTCGGCAAACGT -3’ ADAM9 Forward 5’- AGACAGCCGGGGAGTGTTCCTC -3’ Reverse 5’- AGGTGGCCTTGATGGGAACTGCT-3’ HTRA1 Forward 5’- AGCGACGCCAACACCTACGC -3’ Reverse 5’- CTTCCTGCCCTTGGCCGCAG-3’ MUC13 Forward 5’- CAGAGCCCTTTCTGCGTTGCTTCC -3’ Reverse 5’- GCATCTTCCTGGTAGCCGGGC-3’ MTR Forward 5’-CTTGTGTGGCAGGCTCGCCT -3’ Reverse 5’-AGGTCTTGGAGCGCGGGTGA -3’ MARCKS Forward 5’-GCCAGCATGGGTGCCCAGTTC -3’ Reverse 5’-GTGGCCATTCTCCTGTCCGTTCG -3’ HPSE Forward 5’- GGTGGTTGAGAGCACCAGGCC-3’ Reverse 5’-CCAGCCACATAAAGCCAGCTGCA -3’ PURA Forward 5’-CCGAGGGCACCTCCTTGACTGTG -3’ Reverse 5’-GTGTCCGAACTTGGCCCACACCT -3’ AKAP12 Forward 5’- CCTGCCTTGGGAGTTTGCCGCG-3’ Reverse 5’-CTAGCCGCCGGGTTACGCCT -3’ H19 Forward 5’-CACCAGCTGCCGAAGGCCAA -3’ Reverse 5’-ACAGACCCGCTTCTTGCCGC -3’ ATP7A Forward 5’-GAGTCCCTCATCACAGGGGAGGC -3’ Reverse 5’-GCGCAGATAAGCAGTGACCCG -3’ DDX1 Forward 5’-GGGCTGCTGGTGGTGGAAGC -3’ Reverse 5’-TGCAGGAAAGATGTCTGCGCCT -3’ CYP1B1 Forward 5’-CACCTCCCCGCAGCATGGG -3’ Reverse 5’- CAGTGGCCAGCACCGACAGG-3’ WWOX Forward 5’-CATGGCAAGGGCGAGTGAAGCA -3’ Reverse 5’-AGCACATGAAGAGGCACATTCTTGG -3’ DPYD Forward 5’-GCCATCTTCCAAGGCCTGACGC -3’ Reverse 5’-TGACAGGCGCACATTCCTGCT -3’ ITGA1 Forward 5’-CTCACTGTTGTTCTACGCTGC-3’ Reverse 5’-CGGAGAACCAATAAGCACCCA-3’ ITGA2 Forward 5’- TGTGGTGAGGACGGACTTTG-3’ Reverse 5’- CATCAACCGGCAGGGAGAAT-3’ ITGA3 Forward 5’- ACAGCACCTTCATCGAGGATT-3’ Reverse 5’- CGCCTTCTGCCTCTTAGCTT-3’ ITGA4 Forward 5’- ATCTCTTGGGGCGTTCTTCC-3’ Reverse 5’- CACTAGGAGCCATCGGTTCG-3’ ITGA5 Forward 5’-AAGACTTTCTTGCAGCGGGA-3’ Reverse 5’-GCCACCTGACGCTCTTTTTG-3’ ITGA6 Forward 5’-CAAATGCAGGCACTCAGGTTC-3’ Reverse 5’-GAGCATGGATCTCAGCCTTGT-3’ ITGB1 Forward 5’- GCGCGGAAAAGATGAATTTACA-3’ Reverse 5’- ACATCGTGCAGAAGTAGGCA-3’ ITGB2 Forward 5’-TCAGGACTTTACGACCCGC-3’ Reverse 5’-CCCTGTGAAGTTCAGCTTCTG-3’ ITGB3 Forward 5’- CCGGCCAGATGATTCGAAGA-3’ Reverse 5’- CCAATCCGCAGGTTACTGGT-3’ ITGB4 Forward 5’-GTCTCCGATGACACTGGCTG-3’ Reverse 5’-CCATTCACCAGGTGCTCTCC-3’ ITGB5 Forward 5’-TCGGGGACAACTGTAACTGC-3’ Reverse 5’-GCACTCGACGCAATCTCTCT-3’ siRNA RNF43 siRNA-1 Sense 5’-CAGAACAGAAAGCUAUUAUTT -3’ Antisense 5’-AUAAUAGCUUUCUGUUCUGTT -3’ RNF43 siRNA-2 Sense 5’-GCAGAACAGAAAGCUAUUATT-3’ Antisense 5’-UAAUAGCUUUCUGUUCUGCTT -3’ RNF43 siRNA-3 Sense 5’-GGUGGAGUCUGAAAGAUCATT-3’ Antisense 5’-UGAUCUUUCAGACUCCACCTT -3’ NC siRNA Sense 5’-UUCUCCGAACGUGUCACGUTT -3’ Antisense 5’-ACGUGACACGUUCGGAGAATT -3’ shRNA 5’-CACCGGGCCTTCTGAATGGAGTTCTTTCAAGAGAA RNF43 shRNA Sense GAACTCCATTCAGAAGGCCCTTTTTTG -3’ 5’-GATCCAAAAAAGGGCCTTCTGAATGGAGTTCTTCT Antisense CTTGAAAGAACTCCATTCAGAAGGCCC -3’ 5’-CACCGTTCTCCGAACGTGTCACGTCAAGAGATTAC NC shRNA Sense GTGACACGTTCGGAGAATTTTTTG -3’ 5’-GATCCAAAAAATTCTCCGAACGTGTCACGTAATCTC Antisense TTGACGTGACACGTTCGGAGAAC -3’

Supplementary Table 2. Primary antibodies

Antibodies Assay Origin Dilution Incubate RNF43 IHC #HPA008079, Sigma-Aldrich 1:200 Over-night CD31 IHC #ab28364, AbCam 1:200 Over-night GAPDH WB #SAB1403850, Sigma-Aldrich 1:2000 Over-night RNF43 WB #HPA008079, Sigma-Aldrich 1:1000 Over-night pRB WB #3408-1, Epitomics 1:1000 Over-night Cyclin D1 WB #2261-1, Epitomics 1:1000 Over-night Cyclin D3 WB #2936, Cell Signaling Technology. Inc 1:1000 Over-night CDK2 WB #1134-1, Epitomics 1:1000 Over-night CDK4 WB #3830-1, Epitomics 1:1000 Over-night p53 WB #1047-1, Epitomics 1:1000 Over-night Caspase-3 (Active) WB #1476-1, Epitomics 1:1000 Over-night MMP-1 WB #1976-1, Epitomics 1:1000 Over-night MMP-2 WB #1948-1, Epitomics 1:1000 Over-night MMP-3 WB #1908-1, Epitomics 1:1000 Over-night MMP-9 WB #1939-1, Epitomics 1:1000 Over-night MMP-11 WB #1881-1, Epitomics 1:1000 Over-night MMP-12 WB #1906-1, Epitomics 1:1000 Over-night MMP-13 WB #1923-1, Epitomics 1:1000 Over-night MMP-14 WB #2010-1, Epitomics 1:1000 Over-night MMP-17 WB #2011-1, Epitomics 1:1000 Over-night MMP-21 WB #1955-1, Epitomics 1:1000 Over-night pSTAT3 WB #1121-1, Epitomics 1:1000 Over-night STAT3 WB #3566-1, Epitomics 1:1000 Over-night pAMPK WB #2802-1, Epitomics 1:1000 Over-night AMPK WB #3694-1, Epitomics 1:1000 Over-night E-Cadherin WB #5409-1, Epitomics 1:1000 Over-night pAKT WB #9272, Cell Signaling Technology. Inc 1:1000 Over-night AKT WB #4056, Cell Signaling Technology. Inc 1:1000 Over-night pERK1/2 WB #9106, Cell Signaling Technology. Inc 1:1000 Over-night ERK1/2 WB #9107, Cell Signaling Technology. Inc 1:1000 Over-night

Supplementary Table 3. Differentially expressed genes after RNF43 knockdown

Gene Id Gene Symbol Gene Name FoldΔ p-value Down-regulated genes eukaryotic translation initiation factor 2, subunit 1 alpha, 1965 EIF2S1 0.22 0.0000 35kDa 444 ASPH aspartate beta-hydroxylase 0.23 0.0001 3021 H3F3B H3 histone, family 3B (H3.3B) 0.23 0.0000 guanine nucleotide binding protein (G protein), beta 59345 GNB4 0.24 0.0002 polypeptide 4 57552 NCEH1 arylacetamide deacetylase-like 1 0.24 0.0006 8754 ADAM9 ADAM metallopeptidase domain 9 (meltrin gamma) 0.25 0.0015 5654 HTRA1 HtrA serine peptidase 1 0.25 0.0005 858 CAV2 caveolin 2 0.27 0.0025 ubiquitin-conjugating enzyme E2M (UBC12 homolog, 9040 UBE2M 0.28 0.0000 yeast) 388585 HES5 hairy and enhancer of split 5 (Drosophila) 0.28 0.0052 169200 TMEM64 transmembrane protein 64 0.28 0.0001 8697 CDC23 cell division cycle 23 homolog (S. cerevisiae) 0.29 0.0033 821 CANX calnexin 0.30 0.0005 3915 LAMC1 laminin, gamma 1 (formerly LAMB2) 0.30 0.0003 143384 C10orf46 chromosome 10 open reading frame 46 0.30 0.0001 methylenetetrahydrofolate dehydrogenase (NADP+ 441024 MTHFD2L 0.32 0.0027 dependent) 2-like 133383 C5orf35 chromosome 5 open reading frame 35 0.32 0.0002 3977 LIFR leukemia inhibitory factor receptor alpha 0.32 0.0058 5813 PURA purine-rich element binding protein A 0.32 0.0026 138255 C9orf135 chromosome 9 open reading frame 135 0.33 0.0001 56667 MUC13 mucin 13, cell surface associated 0.33 0.0011 1-acylglycerol-3-phosphate O-acyltransferase 5 55326 AGPAT5 0.33 0.0053 (lysophosphatidic acid acyltransferase, epsilon) 1017 CDK2 cyclin-dependent kinase 2 0.34 0.0012 4884 NPTX1 neuronal pentraxin I 0.34 0.0003 fatty acid binding protein 3, muscle and heart 2170 FABP3 0.35 0.0001 (mammary-derived growth inhibitor) 5-methyltetrahydrofolate-homocysteine 4548 MTR 0.35 0.0084 methyltransferase 10944 C11orf58 chromosome 11 open reading frame 58 0.35 0.0002 5690 PSMB2 proteasome (prosome, macropain) subunit, beta type, 2 0.36 0.0030 2673 GFPT1 glutamine-fructose-6-phosphate transaminase 1 0.36 0.0011 90411 MCFD2 multiple coagulation factor deficiency 2 0.36 0.0002 154141 MBOAT1 membrane bound O-acyltransferase domain containing 1 0.37 0.0042 23731 C9orf5 chromosome 9 open reading frame 5 0.37 0.0033 89884 LHX4 LIM homeobox 4 0.37 0.0004 10114 HIPK3 homeodomain interacting protein kinase 3 0.37 0.0002 440275 EIF2AK4 eukaryotic translation initiation factor 2 alpha kinase 4 0.37 0.0081 25923 ATL3 atlastin GTPase 3 0.37 0.0001 9134 CCNE2 cyclin E2 0.37 0.0090 29970 SCHIP1 schwannomin interacting protein 1 0.38 0.0017 222171 PRR15 proline rich 15 0.38 0.0067 degenerative spermatocyte homolog 1, lipid desaturase 8560 DEGS1 0.38 0.0002 (Drosophila) 25825 BACE2 beta-site APP-cleaving enzyme 2 0.38 0.0000 7107 GPR137B G protein-coupled receptor 137B 0.38 0.0004 adenosine deaminase, RNA-specific, B1 (RED1 104 ADARB1 0.39 0.0071 homolog rat) 493869 GPX8 glutathione peroxidase 8 (putative) 0.39 0.0004 80777 CYB5B cytochrome b5 type B (outer mitochondrial membrane) 0.39 0.0017 7402 UTRN utrophin 0.39 0.0010 4082 MARCKS myristoylated alanine-rich protein kinase C substrate 0.39 0.0070 56605 ERO1LB ERO1-like beta (S. cerevisiae) 0.39 0.0028 8450 CUL4B cullin 4B 0.39 0.0057 84612 PARD6B par-6 partitioning defective 6 homolog beta (C. elegans) 0.40 0.0022 leucine-rich repeat-containing G protein-coupled 55366 LGR4 0.40 0.0027 receptor 4 83595 SOX7 SRY (sex determining region Y)-box 7 0.40 0.0029 6801 STRN striatin, calmodulin binding protein 0.40 0.0010 9581 PREPL prolyl endopeptidase-like 0.41 0.0000 389136 VGLL3 vestigial like 3 (Drosophila) 0.41 0.0026 23011 RAB21 RAB21, member RAS oncogene family 0.41 0.0002 7108 TM7SF2 transmembrane 7 superfamily member 2 0.41 0.0037 56990 CDC42SE2 CDC42 small effector 2 0.41 0.0004 protein phosphatase 2 (formerly 2A), regulatory subunit 5519 PPP2R1B 0.42 0.0015 A, beta isoform 58155 PTBP2 polypyrimidine tract binding protein 2 0.42 0.0005 23040 MYT1L myelin transcription factor 1-like 0.42 0.0081 3691 ITGB4 integrin, beta 4 0.42 0.0000 11009 IL24 interleukin 24 0.42 0.0010 56648 EIF5A2 eukaryotic translation initiation factor 5A2 0.42 0.0002 7368 UGT8 UDP glycosyltransferase 8 0.42 0.0070 9355 LHX2 LIM homeobox 2 0.43 0.0011 115207 KCTD12 potassium channel tetramerisation domain containing 12 0.43 0.0018 7170 TPM3 tropomyosin 3 0.43 0.0025 ASF1 anti-silencing function 1 homolog A (S. 25842 ASF1A 0.43 0.0002 cerevisiae) 253832 ZDHHC20 zinc finger, DHHC-type containing 20 0.44 0.0013 51765 MST4 serine/threonine protein kinase MST4 0.44 0.0001 57121 LPAR5 lysophosphatidic acid receptor 5 0.44 0.0034 4122 MAN2A2 mannosidase, alpha, class 2A, member 2 0.44 0.0079 123606 NIPA1 non imprinted in Prader-Willi/Angelman syndrome 1 0.44 0.0010 219771 CCNY cyclin Y 0.44 0.0034 7247 TSN translin 0.44 0.0044 59271 C21orf63 chromosome 21 open reading frame 63 0.44 0.0098 25871 C3orf17 chromosome 3 open reading frame 17 0.45 0.0014 1028 CDKN1C cyclin-dependent kinase inhibitor 0.45 0.0082 5266 PI3 peptidase inhibitor 3, skin-derived 0.45 0.0094 30849 PIK3R4 phosphoinositide-3-kinase, regulatory subunit 4 0.45 0.0000 2760 GM2A GM2 ganglioside activator 0.45 0.0008 6453 ITSN1 intersectin 1 (SH3 domain protein) 0.45 0.0001 54733 SLC35F2 solute carrier family 35, member F2 0.45 0.0023 ELOVL family member 7, elongation of long chain fatty 79993 ELOVL7 0.45 0.0021 acids (yeast) 10272 FSTL3 follistatin-like 3 (secreted glycoprotein) 0.46 0.0007 29979 UBQLN1 ubiquilin 1 0.46 0.0003 10772 FUSIP1 FUS interacting protein (serine/arginine-rich) 1 0.46 0.0007 7320 UBE2B ubiquitin-conjugating enzyme E2B (RAD6 homolog) 0.46 0.0022 7168 TPM1 tropomyosin 1 (alpha) 0.46 0.0010 UDP-GlcNAc:betaGal 11041 B3GNT1 0.46 0.0010 beta-1,3-N-acetylglucosaminyltransferase 1 394 ARHGAP5 Rho GTPase activating protein 5 0.46 0.0081 58489 FAM108C1 family with sequence similarity 108, member C1 0.46 0.0010 10855 HPSE heparanase 0.46 0.0003 23678 SGK3 serum/glucocorticoid regulated kinase family, member 3 0.46 0.0072 8622 PDE8B phosphodiesterase 8B 0.46 0.0008 7884 SLBP stem-loop binding protein 0.46 0.0054 54491 FAM105A family with sequence similarity 105, member A 0.46 0.0001 283267 LOC283267 hypothetical LOC283267 0.46 0.0049 126969 SLC44A3 solute carrier family 44, member 3 0.47 0.0006 157567 ANKRD46 ankyrin repeat domain 46 0.47 0.0000 54968 TMEM70 transmembrane protein 70 0.47 0.0091 7134 TNNC1 troponin C type 1 (slow) 0.47 0.0001 10228 STX6 syntaxin 6 0.47 0.0033 23167 EFR3A EFR3 homolog A (S. cerevisiae) 0.47 0.0008 57393 TMEM27 transmembrane protein 27 0.47 0.0011 56261 GPCPD1 hypothetical protein KIAA1434 0.47 0.0084 2180 ACSL1 acyl-CoA synthetase long-chain family member 1 0.47 0.0010 v-erb-b2 erythroblastic leukemia viral oncogene 2065 ERBB3 0.48 0.0021 homolog 3 (avian) amyotrophic lateral sclerosis 2 (juvenile) chromosome 65062 ALS2CR4 0.48 0.0098 region, candidate 4 small glutamine-rich tetratricopeptide repeat 54557 SGTB 0.48 0.0027 (TPR)-containing, beta 31 ACACA acetyl-Coenzyme A carboxylase alpha 0.48 0.0008 54897 CASZ1 castor zinc finger 1 0.48 0.0028 53346 TM6SF1 transmembrane 6 superfamily member 1 0.48 0.0026 9590 AKAP12 A kinase (PRKA) anchor protein 12 0.48 0.0001 11046 SLC35D2 solute carrier family 35, member D2 0.48 0.0062 H19, imprinted maternally expressed transcript 283120 H19 0.48 0.0016 (non-protein coding) 23353 UNC84A unc-84 homolog A (C. elegans) 0.48 0.0010 8087 FXR1 fragile X mental retardation, autosomal homolog 1 0.49 0.0009 7078 TIMP3 TIMP metallopeptidase inhibitor 3 0.49 0.0068 538 ATP7A ATPase, Cu++ transporting, alpha polypeptide 0.49 0.0001 10979 FERMT2 fermitin family homolog 2 (Drosophila) 0.49 0.0000 57088 PLSCR4 phospholipid scramblase 4 0.49 0.0032 7323 UBE2D3 ubiquitin-conjugating enzyme E2D 3 0.49 0.0070 9521 EEF1E1 eukaryotic translation elongation factor 1 epsilon 1 0.49 0.0005 10587 TXNRD2 thioredoxin reductase 2 0.49 0.0070 nudix (nucleoside diphosphate linked moiety X)-type 83594 NUDT12 0.49 0.0073 motif 12 283554 GPR137C G protein-coupled receptor 137C 0.49 0.0049 8851 CDK5R1 cyclin-dependent kinase 5, regulatory subunit 1 (p35) 0.50 0.0085 22911 WDR47 WD repeat domain 47 0.50 0.0001 Cas-Br-M (murine) ecotropic retroviral transforming 79872 CBLL1 0.50 0.0079 sequence-like 1 1653 DDX1 DEAD (Asp-Glu-Ala-Asp) box polypeptide 1 0.50 0.0019 55703 POLR3B polymerase (RNA) III (DNA directed) polypeptide B 0.50 0.0016 51535 PPHLN1 periphilin 1 0.50 0.0040 Up-regulated genes 83752 LONP2 lon peptidase 2, peroxisomal 2.00 0.0002 55769 ZNF83 zinc finger protein 83 2.00 0.0005 581 BAX BCL2-associated X protein 2.00 0.0076 91661 ZNF765 zinc finger protein 765 2.03 0.0079 1445 CSK c-src tyrosine kinase 2.03 0.0046 protein phosphatase 1A (formerly 2C), 5494 PPM1A 2.04 0.0057 magnesium-dependent, alpha isoform 84263 HSDL2 hydroxysteroid dehydrogenase like 2 2.04 0.0004 8464 SUPT3H suppressor of Ty 3 homolog (S. cerevisiae) 2.04 0.0050 554313 HIST2H4B histone cluster 2, H4b 2.05 0.0027 26872 STEAP1 six transmembrane epithelial antigen of the prostate 1 2.05 0.0052 29926 GMPPA GDP-mannose pyrophosphorylase A 2.05 0.0063 57711 ZNF529 zinc finger protein 529 2.06 0.0003 554202 LOC554202 hypothetical LOC554202 2.06 0.0032 9321 TRIP11 thyroid hormone receptor interactor 11 2.06 0.0022 79096 C11orf49 chromosome 11 open reading frame 49 2.06 0.0003 79149 ZSCAN5A zinc finger and SCAN domain containing 5A 2.06 0.0084 5886 RAD23A RAD23 homolog A (S. cerevisiae) 2.08 0.0100 80127 C14orf45 chromosome 14 open reading frame 45 2.08 0.0002 6129 RPL7 ribosomal protein L7 2.09 0.0029 643401 LOC643401 hypothetical protein LOC643401 2.10 0.0019 114800 CCDC85A coiled-coil domain containing 85A 2.10 0.0064 8751 ADAM15 ADAM metallopeptidase domain 15 2.10 0.0008 54852 PAQR5 progestin and adipoQ receptor family member V 2.11 0.0008 8343 HIST1H2BF histone cluster 1, H2bf 2.12 0.0010 2887 GRB10 growth factor receptor-bound protein 10 2.12 0.0038 7644 ZNF91 zinc finger protein 91 2.12 0.0024 57107 PDSS2 prenyl (decaprenyl) diphosphate synthase, subunit 2 2.13 0.0000 4199 ME1 malic enzyme 1, NADP(+)-dependent, cytosolic 2.14 0.0016 80821 DDHD1 DDHD domain containing 1 2.15 0.0021 79875 THSD4 thrombospondin, type I, domain containing 4 2.15 0.0033 6711 SPTBN1 spectrin, beta, non-erythrocytic 1 2.16 0.0030 1289 COL5A1 collagen, type V, alpha 1 2.16 0.0040 5099 PCDH7 protocadherin 7 2.16 0.0082 133619 PRRC1 proline-rich coiled-coil 1 2.17 0.0048 121457 IKBIP IKK interacting protein 2.18 0.0003 55275 VPS53 vacuolar protein sorting 53 homolog (S. cerevisiae) 2.18 0.0042 3554 IL1R1 interleukin 1 receptor, type I 2.18 0.0007 3570 IL6R interleukin 6 receptor 2.19 0.0025 92285 ZNF585B zinc finger protein 585B 2.19 0.0011 9183 ZW10 ZW10, kinetochore associated, homolog (Drosophila) 2.21 0.0005 282763 OR51B5 olfactory receptor, family 51, subfamily B, member 5 2.22 0.0013 157574 FBXO16 F-box protein 16 2.22 0.0083 131566 DCBLD2 discoidin, CUB and LCCL domain containing 2 2.25 0.0000 Ras association (RalGDS/AF-6) domain family member 166824 RASSF6 2.28 0.0086 6 84870 RSPO3 R-spondin 3 homolog (Xenopus laevis) 2.28 0.0007 401131 LOC401131 hypothetical LOC401131 2.29 0.0005 26148 C10orf12 chromosome 10 open reading frame 12 2.29 0.0084 3576 IL8 interleukin 8 2.31 0.0000 7586 ZKSCAN1 zinc finger with KRAB and SCAN domains 1 2.34 0.0074 5218 CDK14 PFTAIRE protein kinase 1 2.36 0.0000 8470 SORBS2 sorbin and SH3 domain containing 2 2.36 0.0022 57568 SIPA1L2 signal-induced proliferation-associated 1 like 2 2.37 0.0081 1806 DPYD dihydropyrimidine dehydrogenase 2.37 0.0045 9666 DZIP3 DAZ interacting protein 3, zinc finger 2.38 0.0003 55754 TMEM30A transmembrane protein 30A 2.40 0.0005 645687 C14orf34 chromosome 14 open reading frame 34 2.40 0.0090 13 AADAC arylacetamide deacetylase (esterase) 2.41 0.0062 7086 TKT transketolase 2.43 0.0059 840 CASP7 caspase 7, apoptosis-related cysteine peptidase 2.43 0.0014 2669 GEM GTP binding protein overexpressed in skeletal muscle 2.43 0.0016 5578 PRKCA protein kinase C, alpha 2.46 0.0024 fucosyltransferase 4 (alpha (1,3) fucosyltransferase, 2526 FUT4 2.47 0.0019 myeloid-specific) 23767 FLRT3 fibronectin leucine rich transmembrane protein 3 2.52 0.0002 3624 INHBA inhibin, beta A 2.54 0.0024 1281 COL3A1 collagen, type III, alpha 1 2.54 0.0003 51741 WWOX WW domain containing oxidoreductase 2.55 0.0009 1545 CYP1B1 cytochrome P450, family 1, subfamily B, polypeptide 1 2.56 0.0096 8345 HIST1H2BH histone cluster 1, H2bh 2.56 0.0006 chemokine (C-X-C motif) ligand 1 (melanoma growth 2919 CXCL1 2.58 0.0046 stimulating activity, alpha) 3013 HIST1H2AD histone cluster 1, H2ad 2.58 0.0001 solute carrier family 33 (acetyl-CoA transporter), 9197 SLC33A1 2.59 0.0011 member 1 23299 BICD2 bicaudal D homolog 2 (Drosophila) 2.61 0.0037 114803 MYSM1 Myb-like, SWIRM and MPN domains 1 2.65 0.0011 6038 RNASE4 ribonuclease, RNase A family, 4 2.68 0.0059 72 ACTG2 actin, gamma 2, smooth muscle, enteric 2.70 0.0000 10964 IFI44L interferon-induced protein 44-like 2.71 0.0015 8334 HIST1H2AC histone cluster 1, H2ac 2.72 0.0009 79088 ZNF426 zinc finger protein 426 2.76 0.0023 154791 C7orf55 chromosome 7 open reading frame 55 2.77 0.0039 10028902 LOC100289026 similar to hCG1744891 2.78 0.0012 6 8365 HIST1H4H histone cluster 1, H4h 2.79 0.0039 11098 PRSS23 protease, serine, 23 2.83 0.0002 2801 GOLGA2 golgi autoantigen, golgin subfamily a, 2 2.87 0.0067 284371 ZNF841 zinc finger protein 841 2.88 0.0033 23184 MESDC2 mesoderm development candidate 2 3.03 0.0035 160428 ALDH1L2 aldehyde dehydrogenase 1 family, member L2 3.11 0.0021 23193 GANAB glucosidase, alpha; neutral AB 3.18 0.0004 55033 FKBP14 FK506 binding protein 14, 22 kDa 3.28 0.0010 54885 TBC1D8B TBC1 domain family, member 8B (with GRAM domain) 3.41 0.0000 6696 SPP1 secreted phosphoprotein 1 3.53 0.0002 286499 FAM133A family with sequence similarity 133, member A 3.66 0.0056 90317 ZNF616 zinc finger protein 616 3.68 0.0005 3017 HIST1H2BD histone cluster 1, H2bd 4.83 0.0003 7171 TPM4 tropomyosin 4 5.79 0.0022 9201 DCLK1 doublecortin-like kinase 1 6.67 0.0040

Δ, the fold change column shows the ratio between RNF43-knockdown group and control group.