Troponin C1 ( C, slow skeletal and cardiac muscles, TNNC1)

Catalog number 145324 Supplier United States Biological

Troponin C1(TNNC1) ( type 1 (slow)), also called Troponin C, slow-twitch skeletal muscle or TNC is a that in humans is encoded by the TNNC1 . Using a 'monochromosomal' hybrid panel, the TNNC1 gene is mapped to 3; subsequent analysis of the Genebridge 4 radiation hybrid panel localized the gene to 3p in a region consistent with the earlier assignment. Circular dichroism measurements revealed changes in the secondary structures of the TNNC1 mutants A8V, C84Y, and D145E. Applications Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions Western Blot: 0.1-0.5ug/ml Immunohistochemistry (Paraffin): 0.5-1ug/ml. Boiling the paraffin sections in 10mM citrate buffer, pH 6.0 for 20 minutes is required for the staining of formalin/paraffin sections. Optimal dilutions to be determined by the researcher. Storage and Stability Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Immunogen Synthetic peptide corresponding to aa1-18, MDDIYKAAVEQLTEEQKN at the N-terminal of human Troponin C1. Identical to the related rat and mouse sequences. Formulation Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O. Purity Purified by immunoaffinity chromatography. Specificity Recognizes human Troponin C1. Species Crossreactivity: rat. No crossreactivity with other . Product Type Pab Source human Isotype IgG Grade Affinity Purified Applications IHC WB Crossreactivity Hu Rt Storage -20°C Reference 1. Bermingham, N., Hernandez, D., Balfour, A., Gilmour, F., Martin, J. E., Fisher, E. M. C. Mapping TNNC1, the gene that encodes cardiac in the human and mouse. Genomics 30: 620-622, 1995. 2. Hoffmann, B., Schmidt-Traub, H., Perrot, A., Osterziel, K. J., Gessner, R. First mutation in cardiac troponin C, L29Q, in a patient with hypertrophic cardiomyopathy. Hum. Mutat. 17: 524 only, 2001. 3. Landstrom, A. P., Parvatiyar, M. S., Pinto, J. R., Marquardt, M. L., Bos, J. M., Tester, D. J., Ommen, S. R., Potter, J. D., Ackerman, M. J.Molecular and functional characterization of novel hypertrophic cardiomyopathy susceptibility mutations in TNNC1-encoded troponin C. J. Molec. Cell. Cardiol. 45: 281-288, 2008.

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