Solving Logic Problems with a Twist
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Engineering Transcriptional Control and Synthetic Gene Circuits in Cell Free Systems
University of Tennessee, Knoxville TRACE: Tennessee Research and Creative Exchange Doctoral Dissertations Graduate School 12-2012 Engineering Transcriptional Control and Synthetic Gene Circuits in Cell Free systems Sukanya Iyer University of Tennessee, [email protected] Follow this and additional works at: https://trace.tennessee.edu/utk_graddiss Part of the Biotechnology Commons Recommended Citation Iyer, Sukanya, "Engineering Transcriptional Control and Synthetic Gene Circuits in Cell Free systems. " PhD diss., University of Tennessee, 2012. https://trace.tennessee.edu/utk_graddiss/1586 This Dissertation is brought to you for free and open access by the Graduate School at TRACE: Tennessee Research and Creative Exchange. It has been accepted for inclusion in Doctoral Dissertations by an authorized administrator of TRACE: Tennessee Research and Creative Exchange. For more information, please contact [email protected]. To the Graduate Council: I am submitting herewith a dissertation written by Sukanya Iyer entitled "Engineering Transcriptional Control and Synthetic Gene Circuits in Cell Free systems." I have examined the final electronic copy of this dissertation for form and content and recommend that it be accepted in partial fulfillment of the equirr ements for the degree of Doctor of Philosophy, with a major in Life Sciences. Mitchel J. Doktycz, Major Professor We have read this dissertation and recommend its acceptance: Michael L. Simpson, Albrecht G. von Arnim, Barry D. Bruce, Jennifer L. Morrell-Falvey Accepted for the Council: Carolyn R. Hodges Vice Provost and Dean of the Graduate School (Original signatures are on file with official studentecor r ds.) Engineering Transcriptional Control and Synthetic Gene Circuits in Cell Free systems A Dissertation Presented for the Doctor of Philosophy Degree The University of Tennessee, Knoxville Sukanya Iyer December 2012 Copyright © 2012 by Sukanya Iyer. -
CSE Yet, Please Do Well! Logical Connectives
administrivia Course web: http://www.cs.washington.edu/311 Office hours: 12 office hours each week Me/James: MW 10:30-11:30/2:30-3:30pm or by appointment TA Section: Start next week Call me: Shayan Don’t: Actually call me. Homework #1: Will be posted today, due next Friday by midnight (Oct 9th) Gradescope! (stay tuned) Extra credit: Not required to get a 4.0. Counts separately. In total, may raise grade by ~0.1 Don’t be shy (raise your hand in the back)! Do space out your participation. If you are not CSE yet, please do well! logical connectives p q p q p p T T T T F T F F F T F T F NOT F F F AND p q p q p q p q T T T T T F T F T T F T F T T F T T F F F F F F OR XOR 푝 → 푞 • “If p, then q” is a promise: p q p q F F T • Whenever p is true, then q is true F T T • Ask “has the promise been broken” T F F T T T If it’s raining, then I have my umbrella. related implications • Implication: p q • Converse: q p • Contrapositive: q p • Inverse: p q How do these relate to each other? How to see this? 푝 ↔ 푞 • p iff q • p is equivalent to q • p implies q and q implies p p q p q Let’s think about fruits A fruit is an apple only if it is either red or green and a fruit is not red and green. -
Digital IC Listing
BELS Digital IC Report Package BELS Unit PartName Type Location ID # Price Type CMOS 74HC00, Quad 2-Input NAND Gate DIP-14 3 - A 500 0.24 74HCT00, Quad 2-Input NAND Gate DIP-14 3 - A 501 0.36 74HC02, Quad 2 Input NOR DIP-14 3 - A 417 0.24 74HC04, Hex Inverter, buffered DIP-14 3 - A 418 0.24 74HC04, Hex Inverter (buffered) DIP-14 3 - A 511 0.24 74HCT04, Hex Inverter (Open Collector) DIP-14 3 - A 512 0.36 74HC08, Quad 2 Input AND Gate DIP-14 3 - A 408 0.24 74HC10, Triple 3-Input NAND DIP-14 3 - A 419 0.31 74HC32, Quad OR DIP-14 3 - B 409 0.24 74HC32, Quad 2-Input OR Gates DIP-14 3 - B 543 0.24 74HC138, 3-line to 8-line decoder / demultiplexer DIP-16 3 - C 603 1.05 74HCT139, Dual 2-line to 4-line decoders / demultiplexers DIP-16 3 - C 605 0.86 74HC154, 4-16 line decoder/demulitplexer, 0.3 wide DIP - Small none 445 1.49 74HC154W, 4-16 line decoder/demultiplexer, 0.6wide DIP none 446 1.86 74HC190, Synchronous 4-Bit Up/Down Decade and Binary Counters DIP-16 3 - D 637 74HCT240, Octal Buffers and Line Drivers w/ 3-State outputs DIP-20 3 - D 643 1.04 74HC244, Octal Buffers And Line Drivers w/ 3-State outputs DIP-20 3 - D 647 1.43 74HCT245, Octal Bus Transceivers w/ 3-State outputs DIP-20 3 - D 649 1.13 74HCT273, Octal D-Type Flip-Flops w/ Clear DIP-20 3 - D 658 1.35 74HCT373, Octal Transparent D-Type Latches w/ 3-State outputs DIP-20 3 - E 666 1.35 74HCT377, Octal D-Type Flip-Flops w/ Clock Enable DIP-20 3 - E 669 1.50 74HCT573, Octal Transparent D-Type Latches w/ 3-State outputs DIP-20 3 - E 674 0.88 Type CMOS CD4000 Series CD4001, Quad 2-input -
The Equation for the 3-Input XOR Gate Is Derived As Follows
The equation for the 3-input XOR gate is derived as follows The last four product terms in the above derivation are the four 1-minterms in the 3-input XOR truth table. For 3 or more inputs, the XOR gate has a value of 1when there is an odd number of 1’s in the inputs, otherwise, it is a 0. Notice also that the truth tables for the 3-input XOR and XNOR gates are identical. It turns out that for an even number of inputs, XOR is the inverse of XNOR, but for an odd number of inputs, XOR is equal to XNOR. All these gates can be interconnected together to form large complex circuits which we call networks. These networks can be described graphically using circuit diagrams, with Boolean expressions or with truth tables. 3.2 Describing Logic Circuits Algebraically Any logic circuit, no matter how complex, may be completely described using the Boolean operations previously defined, because of the OR gate, AND gate, and NOT circuit are the basic building blocks of digital systems. For example consider the circuit shown in Figure 1.3(c). The circuit has three inputs, A, B, and C, and a single output, x. Utilizing the Boolean expression for each gate, we can easily determine the expression for the output. The expression for the AND gate output is written A B. This AND output is connected as an input to the OR gate along with C, another input. The OR gate operates on its inputs such that its output is the OR sum of the inputs. -
Hardware Abstract the Logic Gates References Results Transistors Through the Years Acknowledgements
The Practical Applications of Logic Gates in Computer Science Courses Presenters: Arash Mahmoudian, Ashley Moser Sponsored by Prof. Heda Samimi ABSTRACT THE LOGIC GATES Logic gates are binary operators used to simulate electronic gates for design of circuits virtually before building them with-real components. These gates are used as an instrumental foundation for digital computers; They help the user control a computer or similar device by controlling the decision making for the hardware. A gate takes in OR GATE AND GATE NOT GATE an input, then it produces an algorithm as to how The OR gate is a logic gate with at least two An AND gate is a consists of at least two A NOT gate, also known as an inverter, has to handle the output. This process prevents the inputs and only one output that performs what inputs and one output that performs what is just a single input with rather simple behavior. user from having to include a microprocessor for is known as logical disjunction, meaning that known as logical conjunction, meaning that A NOT gate performs what is known as logical negation, which means that if its input is true, decision this making. Six of the logic gates used the output of this gate is true when any of its the output of this gate is false if one or more of inputs are true. If all the inputs are false, the an AND gate's inputs are false. Otherwise, if then the output will be false. Likewise, are: the OR gate, AND gate, NOT gate, XOR gate, output of the gate will also be false. -
Designing Combinational Logic Gates in Cmos
CHAPTER 6 DESIGNING COMBINATIONAL LOGIC GATES IN CMOS In-depth discussion of logic families in CMOS—static and dynamic, pass-transistor, nonra- tioed and ratioed logic n Optimizing a logic gate for area, speed, energy, or robustness n Low-power and high-performance circuit-design techniques 6.1 Introduction 6.3.2 Speed and Power Dissipation of Dynamic Logic 6.2 Static CMOS Design 6.3.3 Issues in Dynamic Design 6.2.1 Complementary CMOS 6.3.4 Cascading Dynamic Gates 6.5 Leakage in Low Voltage Systems 6.2.2 Ratioed Logic 6.4 Perspective: How to Choose a Logic Style 6.2.3 Pass-Transistor Logic 6.6 Summary 6.3 Dynamic CMOS Design 6.7 To Probe Further 6.3.1 Dynamic Logic: Basic Principles 6.8 Exercises and Design Problems 197 198 DESIGNING COMBINATIONAL LOGIC GATES IN CMOS Chapter 6 6.1Introduction The design considerations for a simple inverter circuit were presented in the previous chapter. In this chapter, the design of the inverter will be extended to address the synthesis of arbitrary digital gates such as NOR, NAND and XOR. The focus will be on combina- tional logic (or non-regenerative) circuits that have the property that at any point in time, the output of the circuit is related to its current input signals by some Boolean expression (assuming that the transients through the logic gates have settled). No intentional connec- tion between outputs and inputs is present. In another class of circuits, known as sequential or regenerative circuits —to be dis- cussed in a later chapter—, the output is not only a function of the current input data, but also of previous values of the input signals (Figure 6.1). -
Class 10: CMOS Gate Design
Class 10: CMOS Gate Design Topics: 1. Exclusive OR Implementation 2. Exclusive OR Carry Circuit 3. PMOS Carry Circuit Equivalent 4. CMOS Full-Adder 5. NAND, NOR Gate Considerations 6. Logic Example 7. Logic Negation 8. Mapping Logic ‘0’ 9. Equivalent Circuits 10. Fan-In and Fan-Out 11. Rise Delay Time 12. Rise Delay Time 13. Rise Delay Time 14. Fall Delay Time 15. Equal Delays Joseph A. Elias, PhD 1 Class 10: CMOS Gate Design Exclusive OR Design (Martin c4.5) 3-input XOR Truth Table Similar to how one derives a 2-input XOR (Martin, p.183) using (a’+b’)=(ab)’ (a’b’)=(a+b)’ a XOR b = a’b + ab’ = a’a + a’b + ab’ + bb’ = a’(a+b) + b’(a+b) = (a’+b’)(a+b) = (ab)’(a+b) = (ab + (a’b’) )’ = (ab + (a + b)’ )’ Joseph A. Elias, PhD 2 Class 10: CMOS Gate Design Exclusive OR Carry Circuit (Martin c4.5) NMOS realization PMOS equivalent •A in parallel with B •A in series with B •A||B in series with C •AB in parallel with C •AB in parallel with (A||B)C •A||B in series with (AB)||C Vout = Joseph A. Elias, PhD 3 Class 10: CMOS Gate Design PMOS Carry Circuit Equivalent (Martin c4.5) •Martin indicates equivalency between these circuits •Is this true? (AB+C)(A+B) = (A+B)C + (AB) ABA + ABB + AC + BC = AC +BC +AB AB + AB + AC + BC = AC + BC + AB AB + AC + BC = AB + AC + BC equivalent Joseph A. Elias, PhD 4 Class 10: CMOS Gate Design CMOS Full-Adder (Martin c4.5) Sum: (A+B+C) Carry + ABC Carry: (A+B)C + AB Joseph A. -
–Not –And –Or –Xor –Nand –Nor
Gates Six types of gates –NOT –AND –OR –XOR –NAND –NOR 1 NOT Gate A NOT gate accepts one input signal (0 or 1) and returns the complementary (opposite) signal as output 2 AND Gate An AND gate accepts two input signals If both are 1, the output is 1; otherwise, the output is 0 3 OR Gate An OR gate accepts two input signals If both are 0, the output is 0; otherwise, the output is 1 4 XOR Gate An XOR gate accepts two input signals If both are the same, the output is 0; otherwise, the output is 1 5 XOR Gate Note the difference between the XOR gate and the OR gate; they differ only in one input situation When both input signals are 1, the OR gate produces a 1 and the XOR produces a 0 XOR is called the exclusive OR because its output is 1 if (and only if): • either one input or the other is 1, • excluding the case that they both are 6 NAND Gate The NAND (“NOT of AND”) gate accepts two input signals If both are 1, the output is 0; otherwise, the output is 1 NOR Gate The NOR (“NOT of OR”) gate accepts two inputs If both are 0, the output is 1; otherwise, the output is 0 8 AND OR XOR NAND NOR 9 Review of Gate Processing Gate Behavior NOT Inverts its single input AND Produces 1 if all input values are 1 OR Produces 0 if all input values are 0 XOR Produces 0 if both input values are the same NAND Produces 0 if all input values are 1 NOR Produces 1 if all input values are 0 10 Combinational Circuits Gates are combined into circuits by using the output of one gate as the input for another This same circuit using a Boolean expression is AB + AC 11 Combinational Circuits Three inputs require eight rows to describe all possible input combinations 12 Combinational Circuits Consider the following Boolean expression A(B + C) Does this truth table look familiar? Compare it with previous table 13. -
Combinational Logic Circuits
CHAPTER 4 COMBINATIONAL LOGIC CIRCUITS ■ OUTLINE 4-1 Sum-of-Products Form 4-10 Troubleshooting Digital 4-2 Simplifying Logic Circuits Systems 4-3 Algebraic Simplification 4-11 Internal Digital IC Faults 4-4 Designing Combinational 4-12 External Faults Logic Circuits 4-13 Troubleshooting Prototyped 4-5 Karnaugh Map Method Circuits 4-6 Exclusive-OR and 4-14 Programmable Logic Devices Exclusive-NOR Circuits 4-15 Representing Data in HDL 4-7 Parity Generator and Checker 4-16 Truth Tables Using HDL 4-8 Enable/Disable Circuits 4-17 Decision Control Structures 4-9 Basic Characteristics of in HDL Digital ICs M04_WIDM0130_12_SE_C04.indd 136 1/8/16 8:38 PM ■ CHAPTER OUTCOMES Upon completion of this chapter, you will be able to: ■■ Convert a logic expression into a sum-of-products expression. ■■ Perform the necessary steps to reduce a sum-of-products expression to its simplest form. ■■ Use Boolean algebra and the Karnaugh map as tools to simplify and design logic circuits. ■■ Explain the operation of both exclusive-OR and exclusive-NOR circuits. ■■ Design simple logic circuits without the help of a truth table. ■■ Describe how to implement enable circuits. ■■ Cite the basic characteristics of TTL and CMOS digital ICs. ■■ Use the basic troubleshooting rules of digital systems. ■■ Deduce from observed results the faults of malfunctioning combina- tional logic circuits. ■■ Describe the fundamental idea of programmable logic devices (PLDs). ■■ Describe the steps involved in programming a PLD to perform a simple combinational logic function. ■■ Describe hierarchical design methods. ■■ Identify proper data types for single-bit, bit array, and numeric value variables. -
SN74LVC1G04 Single Inverter Gate Datasheet (Rev
Product Sample & Technical Tools & Support & Folder Buy Documents Software Community SN74LVC1G04 SCES214AD–APRIL1999–REVISED OCTOBER 2014 SN74LVC1G04 Single Inverter Gate 1 Features 3 Description 2 1• Available in the Ultra-Small 0.64-mm This single inverter gate is designed for Package (DPW) with 0.5-mm Pitch 1.65-V to 5.5-V VCC operation. • Supports 5-V VCC Operation The SN74LVC1G04 device performs the Boolean • Inputs Accept Voltages up to 5.5 V Allowing Down function Y = A. Translation to VCC The CMOS device has high output drive while maintaining low static power dissipation over a broad • Max tpd of 3.3 ns at 3.3-V VCC operating range. • Low Power Consumption, 10-μA Max ICC • ±24-mA Output Drive at 3.3-V The SN74LVC1G04 device is available in a variety of packages, including the ultra-small DPW package •Ioff Supports Live-Insertion, Partial-Power-Down with a body size of 0.8 mm × 0.8 mm. Mode, and Back-Drive Protection • Latch-Up Performance Exceeds 100 mA Device Information(1) Per JESD 78, Class II DEVICE NAME PACKAGE BODY SIZE • ESD Protection Exceeds JESD 22 SOT-23 (5) 2.9mm × 1.6mm – 2000-V Human-Body Model (A114-A) SC70 (5) 2.0mm × 1.25mm – 200-V Machine Model (A115-A) SN74LVC1G04 SON (6) 1.45mm × 1.0mm – 1000-V Charged-Device Model (C101) SON (6) 1.0mm × 1.0mm X2SON (4) 0.8mm × 0.8mm 2 Applications (1) For all available packages, see the orderable addendum at the end of the datasheet. • AV Receiver • Audio Dock: Portable • Blu-ray Player and Home Theater • Embedded PC • MP3 Player/Recorder (Portable Audio) • Personal Digital Assistant (PDA) • Power: Telecom/Server AC/DC Supply: Single Controller: Analog and Digital • Solid State Drive (SSD): Client and Enterprise • TV: LCD/Digital and High-Definition (HDTV) • Tablet: Enterprise • Video Analytics: Server • Wireless Headset, Keyboard, and Mouse 4 Simplified Schematic A Y 1 An IMPORTANT NOTICE at the end of this data sheet addresses availability, warranty, changes, use in safety-critical applications, intellectual property matters and other important disclaimers. -
Design and Simulation of V&Pl Submodules Using Nand And
DESIGN AND SIMULATION OF V&PL SUBMODULES USING NAND AND NOR GATE H.Hasim, Syirrazie CS Instrumentation and Automation Center, Technical Support Division, Malaysia Nuclear Agensi 43000, Bangi, Kajang, Selangor Abstract Digital Integarted Circuit(IC) design is an alternative to current analog IC design. Both have vise verse advan- tages and disadvantages. This paper investigate and compare sub-modules of voting and protecting logic (V&PL) using only two input either NAND gate(74LS00) or NOR gate (74LS02). On the previous research, V&PL sub- module are mixed of six input NOR gate, 2 input AND gate, and Inverter. The result show a complexity of circuity and difficulty of finding six input NOR gate. We extend this analysis by comparable of less complexity of PMOS and NMOS used in NAND and NOR gate as V&PL sub-module, less time propagation delay, with high frequency and less total nombor of transistor used. Kanaugh map minimizer, logisim, and LTSpice used to design and develop of V&PL sub-module Complementary Metal Oxide Semiconductor(CMOS) NAND and NOR gate.Result show that, propagation delay of NAND gate is less with high frequency than NOR gate. Keywords: CMOS, NOR gate, NAND gate, Voting and Protecting Logic(V&PL) 1 Introduction One of the key challenges in IC design is making used of transistor. Transistor can be either biasing and funtion as amplifier or operated in saturated and cut-off region. To be digitalize, transistor will operated in saturated and cut-off region. The saturated region is when the flat or staedy-state amount of voltage throught out of time while in cut-off region it is approximately zeros volt. -
Logic Families
Logic Families PDF generated using the open source mwlib toolkit. See http://code.pediapress.com/ for more information. PDF generated at: Mon, 11 Aug 2014 22:42:35 UTC Contents Articles Logic family 1 Resistor–transistor logic 7 Diode–transistor logic 10 Emitter-coupled logic 11 Gunning transceiver logic 16 Transistor–transistor logic 16 PMOS logic 23 NMOS logic 24 CMOS 25 BiCMOS 33 Integrated injection logic 34 7400 series 35 List of 7400 series integrated circuits 41 4000 series 62 List of 4000 series integrated circuits 69 References Article Sources and Contributors 75 Image Sources, Licenses and Contributors 76 Article Licenses License 77 Logic family 1 Logic family In computer engineering, a logic family may refer to one of two related concepts. A logic family of monolithic digital integrated circuit devices is a group of electronic logic gates constructed using one of several different designs, usually with compatible logic levels and power supply characteristics within a family. Many logic families were produced as individual components, each containing one or a few related basic logical functions, which could be used as "building-blocks" to create systems or as so-called "glue" to interconnect more complex integrated circuits. A "logic family" may also refer to a set of techniques used to implement logic within VLSI integrated circuits such as central processors, memories, or other complex functions. Some such logic families use static techniques to minimize design complexity. Other such logic families, such as domino logic, use clocked dynamic techniques to minimize size, power consumption, and delay. Before the widespread use of integrated circuits, various solid-state and vacuum-tube logic systems were used but these were never as standardized and interoperable as the integrated-circuit devices.