DU PhD in Plant Molecular Biology N Biotech
Topic:- DU_J18_PHD_PMBB
1) Half-life of 32Phosphorous is
[Question ID = 52689]
1. 60.4 days. [Option ID = 90750] 2. 14.3 days. [Option ID = 90751] 3. 12.4 years. [Option ID = 90748] 4. 87.5 years. [Option ID = 90749]
Correct Answer :- 14.3 days. [Option ID = 90751]
2) Retrograde vesicular transport takes place in which of the following organelles in plant cell? [Question ID = 52718]
1. ER, Golgi bodies and plasma membrane [Option ID = 90866] 2. ER, Golgi bodies and lysosome [Option ID = 90865] 3. Golgi bodies, lysosome and peroxisomes [Option ID = 90867] 4. ER, Vesicular tubules and Golgi bodies [Option ID = 90864]
Correct Answer :- ER, Vesicular tubules and Golgi bodies [Option ID = 90864]
3) In a run-on assay, mRNA level is higher by two folds in hormone treated sample. Keeping this outcome in view, which of the following statement is correct? [Question ID = 52686]
1. Hormone binds RNA polymerase [Option ID = 90738] 2. Hormone stabilizes mRNA [Option ID = 90736] 3. Hormone increases synthesis of RNA polymerase [Option ID = 90739] 4. Hormone stimulates mRNA synthesis [Option ID = 90737]
Correct Answer :- Hormone stimulates mRNA synthesis [Option ID = 90737]
4) The action of which of the following enzymes on phosphoinositide bisphosphate would produce phosphatidic acid? [Question ID = 52702]
1. Phospholipase D [Option ID = 90803] 2. Phospholipase C [Option ID = 90802] 3. Phospholipase A1 [Option ID = 90800] 4. Phospholipase A2 [Option ID = 90801]
Correct Answer :- Phospholipase D [Option ID = 90803] 5) The yeast ‘petite’ mutant and ‘poky’ mutant of bread-mold have defect in genes involved in [Question ID = 52717]
1. Calvin cycle. [Option ID = 90863] 2. glycolysis reactions. [Option ID = 90860] 3. citiric acid cycle. [Option ID = 90861] 4. electron transport chain. [Option ID = 90862]
Correct Answer :- electron transport chain. [Option ID = 90862]
6) I-V curve, inward and outward -rectifiers terminologies are used in [Question ID = 52719]
1. vesicular transport of proteins in the cell. [Option ID = 90868] 2. cell Fractionation techniques. [Option ID = 90871] 3. microscopy techniques and methodologies. [Option ID = 90869] 4. electrophysiology techniques. [Option ID = 90870]
Correct Answer :- electrophysiology techniques. [Option ID = 90870]
7) Poly A tail is added to the transcript by [Question ID = 52690]
1. poly A polymerase using DNA as a template. [Option ID = 90753] 2. poly A polymerase post-transcriptionally. [Option ID = 90755] 3. DNA polymerase using DNA as a template. [Option ID = 90752] 4. RNA polymerase post-transcriptionally. [Option ID = 90754]
Correct Answer :- RNA polymerase post-transcriptionally. [Option ID = 90754]
8) Nobel Prize for the discovery of RNAi was shared between [Question ID = 52721]
1. Hargobind Khurana and Susumu Tonegawa. [Option ID = 90879] 2. Thomas Cech and Sidney Altman. [Option ID = 90877] 3. Craig Mello and Andrew Fire. [Option ID = 90876] 4. James Watson and Francis Crick. [Option ID = 90878]
Correct Answer :- Craig Mello and Andrew Fire. [Option ID = 90876]
9) The source of GFP reporter protein is [Question ID = 52707]
1. firefly. [Option ID = 90823] 2. jelly fish. [Option ID = 90821] 3. mollusc. [Option ID = 90820] 4. earthworm. [Option ID = 90822]
Correct Answer :- jelly fish. [Option ID = 90821]
10) ‘Okazaki fragments’ are synthesized [Question ID = 52732] 1. in the leading strand. [Option ID = 90920] 2. in the lagging strand. [Option ID = 90921] 3. in both leading and lagging strands. [Option ID = 90922] 4. only during initiation of replication. [Option ID = 90923]
Correct Answer :- in the lagging strand. [Option ID = 90921]
11) Glutathione consists of [Question ID = 52698]
1. glycine, cysteine and glutamate. [Option ID = 90785] 2. glutamate, glutamine and glycine. [Option ID = 90786] 3. glutamate, glutamine and cysteine. [Option ID = 90784] 4. aspartate, glutamine and cysteine. [Option ID = 90787]
Correct Answer :- glycine, cysteine and glutamate. [Option ID = 90785]
12) If thylakoids of chloroplasts are punctured, which of the following processes would be directly affected? [Question ID = 52704]
1. Synthesis of ATP [Option ID = 90810] 2. Splitting of water [Option ID = 90808] 3. Reduction of NADP+ [Option ID = 90811] 4. Flow of electrons from PSII to PSI [Option ID = 90809]
Correct Answer :- Synthesis of ATP [Option ID = 90810]
13) Ethyl methane sulfonate (EMS) produces mutations because it [Question ID = 52729]
1. is an reducing agent affecting the phosphodiester linkage. [Option ID = 90909] 2. produces deamination causing mis-pairing of bases. [Option ID = 90910] 3. is an oxidizing agent and causes double-bond formation. [Option ID = 90908] 4. is an alkylating agent causing mis-pairing of bases. [Option ID = 90911]
Correct Answer :- is an alkylating agent causing mis-pairing of bases. [Option ID = 90911]
14) Proto-oncogenes are [Question ID = 52694]
1. primitive oncogenes. [Option ID = 90770] 2. normal cellular genes. [Option ID = 90768] 3. cancer promoting genes. [Option ID = 90769] 4. portable oncogenes. [Option ID = 90771]
Correct Answer :- normal cellular genes. [Option ID = 90768]
15) Who showed that chloroplasts can synthesize ATP even in dark if artificial pH gradient is imposed across the thylakoid membrane? [Question ID = 52697] 1. Robert Emerson [Option ID = 90783] 2. Melvin Calvin [Option ID = 90782] 3. Andre Jagendorf [Option ID = 90780] 4. Robert Hill [Option ID = 90781]
Correct Answer :- Andre Jagendorf [Option ID = 90780]
16) A messenger RNA with a 336 nucleotide coding sequence (that includes the initiation and a termination codon) would translate into a polypeptide of [Question ID = 52726]
1. 110 amino acids. [Option ID = 90898] 2. 111 amino acids. [Option ID = 90899] 3. 990 amino acids. [Option ID = 90897] 4. 999 amino acids. [Option ID = 90896]
Correct Answer :- 111 amino acids. [Option ID = 90899]
17) Which type of multi-subunit endonucleases cut double stranded DNA at random, far from their recognition sequence in either direction? [Question ID = 52724]
1. Type II [Option ID = 90889] 2. Type III [Option ID = 90890] 3. Type IV [Option ID = 90891] 4. Type I [Option ID = 90888]
Correct Answer :- Type I [Option ID = 90888]
18) The enzyme for conversion from X-p to X and phosphate is called [Question ID = 52709]
1. kinase. [Option ID = 90828] 2. phosphatase. [Option ID = 90829] 3. phosphotransferase. [Option ID = 90831] 4. reductase. [Option ID = 90830]
Correct Answer :- phosphatase. [Option ID = 90829]
19) The enzyme that catalyzes the splitting of PIP2 into molecules of inositol triphosphate (IP3) and diacylglycerol in cell signaling, is [Question ID = 52713]
1. phosphodiesterase C. [Option ID = 90846] 2. phospholipase C. [Option ID = 90845] 3. phosphokinase C. [Option ID = 90844] 4. Phosphotransferase C. [Option ID = 90847]
Correct Answer :- phospholipase C. [Option ID = 90845]
20) The Klenow fragment of DNA polymerase I lacks [Question ID = 52728] 1. both 3’-5’ exonuclease and 5’-3’ polymerase activity. [Option ID = 90907] 2. 5’-3’ exonuclease activity. [Option ID = 90904] 3. 3’-5’ exonuclease activity. [Option ID = 90905] 4. 5’-3’ polymerase activity. [Option ID = 90906]
Correct Answer :- 5’-3’ exonuclease activity. [Option ID = 90904]
21) What is the approximate size of the Ti plasmid in Agrobacterium tumefaciens? [Question ID = 52705]
1. 10 kb [Option ID = 90813] 2. 20 kb [Option ID = 90814] 3. 3 kb [Option ID = 90812] 4. 200 kb [Option ID = 90815]
Correct Answer :- 200 kb [Option ID = 90815]
22) Major stabilizing force for tertiary structure of protein is [Question ID = 52688]
1. hydrophobic interaction. [Option ID = 90744] 2. peptide bond. [Option ID = 90747] 3. hydrogen bond. [Option ID = 90745] 4. alpha-helix. [Option ID = 90746]
Correct Answer :- hydrophobic interaction. [Option ID = 90744]
23) Expansion of MALDI-TOF is [Question ID = 52692]
1. Matrix-assisted laser desorption/ionization time-of-flight. [Option ID = 90760] 2. Matrix-assisted laser desorption/ionization time-of-fission. [Option ID = 90762] 3. Matrix-assisted laser desorption/ionization time-of-fusion. [Option ID = 90761] 4. Matrix-assisted light desorption/ionization time-of-flight. [Option ID = 90763]
Correct Answer :- Matrix-assisted laser desorption/ionization time-of-flight. [Option ID = 90760]
24) The drug rifampicin inhibits transcription by binding to [Question ID = 52720]
1. sigma subunit of RNA polymerase. [Option ID = 90873] 2. alpha subunit of RNA polymerase. [Option ID = 90872] 3. beta subunit of RNA polymerase. [Option ID = 90874] 4. gamma subunit of RNA polymerase. [Option ID = 90875]
Correct Answer :- beta subunit of RNA polymerase. [Option ID = 90874]
25) 18 If green alga is provided with H2O labelled with O, and kept in photosynthetic conditions, which of the following photosynthetic products would not carry 18O? [Question ID = 52703] 1. RuBP [Option ID = 90806] 2. Glucose [Option ID = 90805] 3. None of these [Option ID = 90807] 4. PGA [Option ID = 90804]
Correct Answer :- None of these [Option ID = 90807]
26) Small nucleolar RNAs are involved in [Question ID = 52691]
1. mRNA processing. [Option ID = 90759] 2. tRNA processing. [Option ID = 90758] 3. rRNA processing. [Option ID = 90756] 4. small RNA processing. [Option ID = 90757]
Correct Answer :- rRNA processing. [Option ID = 90756]
27) Following is an example of lipids where hydroxyl- or epoxy-fatty acids are joined by ester linkage: [Question ID = 52701]
1. Triacylglycerol [Option ID = 90798] 2. Suberin [Option ID = 90799] 3. Phosphatidyl serine [Option ID = 90797] 4. Phosphatidyl inositol [Option ID = 90796]
Correct Answer :- Suberin [Option ID = 90799]
28) Reduction of nitrite to ammonia takes place in [Question ID = 52700]
1. chloroplast. [Option ID = 90792] 2. glyoxysome. [Option ID = 90794] 3. cytosol. [Option ID = 90795] 4. peroxisome. [Option ID = 90793]
Correct Answer :- chloroplast. [Option ID = 90792]
29) Movement of which of the following in photosystem II is affected by phosphorylation? [Question ID = 52695]
1. D2 protein of PSII [Option ID = 90773] 2. D1 protein of PSII [Option ID = 90772] 3. LHCP [Option ID = 90774] 4. Plastoquinone [Option ID = 90775]
Correct Answer :- LHCP [Option ID = 90774]
30) Which of the following is a hormone whose action requires a cell surface receptor? [Question ID = 52714] 1. Insulin [Option ID = 90850] 2. Progesterone [Option ID = 90849] 3. Nitric oxide [Option ID = 90848] 4. Estrogen [Option ID = 90851]
Correct Answer :- Insulin [Option ID = 90850]
31) When both glucose and lactose are present in the growth medium, E. coli cells use [Question ID = 52733]
1. alternatively glucose and lactose for short periods. [Option ID = 90925] 2. first lactose till it is exhausted, then glucose. [Option ID = 90926] 3. first glucose till it is exhausted, then lactose. [Option ID = 90927] 4. both glucose and lactose together. [Option ID = 90924]
Correct Answer :- first glucose till it is exhausted, then lactose. [Option ID = 90927]
32) Cry1 endotoxins, synthesized by soil bacterium Bacillus thuringiensis are effective against [Question ID = 52725]
1. annelids. [Option ID = 90895] 2. lepidoptera larvae. [Option ID = 90893] 3. nematodes. [Option ID = 90894] 4. diptera larvae. [Option ID = 90892]
Correct Answer :- lepidoptera larvae. [Option ID = 90893]
33) Which step of translation does NOT require GTP hydrolysis? [Question ID = 52731]
1. Assembly of ribosome subunits at the S-D sequence [Option ID = 90918] 2. Dissociation of ribosome subunits at stop codon. [Option ID = 90919] 3. Peptide bond formation [Option ID = 90916] 4. Translocation [Option ID = 90917]
Correct Answer :- Peptide bond formation [Option ID = 90916]
34) MicroRNA genes are primarily transcribed by [Question ID = 52735]
1. DNA polymerase. [Option ID = 90935] 2. RNA pol II. [Option ID = 90933] 3. RNA pol III. [Option ID = 90934] 4. RNA pol I. [Option ID = 90932]
Correct Answer :- RNA pol II. [Option ID = 90933]
35) Which of the following factors involved in symbiotic nitrogen fixation is of bacterial origin? [Question ID = 52699] 1. Nod D [Option ID = 90791] 2. LysM-RLK [Option ID = 90789] 3. Leghaemoglobin [Option ID = 90788] 4. Uricase [Option ID = 90790]
Correct Answer :- Nod D [Option ID = 90791]
36) Which of the following metal ion acts as a versatile signaling molecule in biological system? [Question ID = 52708]
1. Ca2+ [Option ID = 90826] 2. Mn2+ [Option ID = 90824] 3. Na+ [Option ID = 90827] 4. Mg2+ [Option ID = 90825]
Correct Answer :- Ca2+ [Option ID = 90826]
37) Which of the following forces are responsible for binding of a metal to protein in a biological system? [Question ID = 52710]
1. Coordinate bonds [Option ID = 90834] 2. Ionic bonds [Option ID = 90833] 3. Covalent bonds [Option ID = 90832] 4. Hydrogen bonds [Option ID = 90835]
Correct Answer :- Coordinate bonds [Option ID = 90834]
38) Which of the following mobile electron carrier can accept two electrons for its reduction during photosynthesis? [Question ID = 52696]
1. Plastocyanin. [Option ID = 90779] 2. Phaeophytin. [Option ID = 90777] 3. Ferredoxin [Option ID = 90778] 4. Plastoquinone [Option ID = 90776]
Correct Answer :- Plastoquinone [Option ID = 90776]
39) Which of the following primers would allow copying of the single-stranded DNA sequence 5’- ATGCCTAGGTC-3’? [Question ID = 52723]
1. 5’-TACGG-3’ [Option ID = 90884] 2. 5’-GGCAT-3’ [Option ID = 90886] 3. 5’-CTGGA-3’ [Option ID = 90885] 4. 5’-GACCT-3’ [Option ID = 90887]
Correct Answer :- 5’-GACCT-3’ [Option ID = 90887] 40) Which of the following statements about G proteins is FALSE? [Question ID = 52712]
1. They become activated when bound to GDP [Option ID = 90842] 2. They become activated when bound to GTP [Option ID = 90843] 3. They bind to and are regulated by guanine nucleotides [Option ID = 90841] 4. They are involved in signal cascades [Option ID = 90840]
Correct Answer :- They become activated when bound to GDP [Option ID = 90842]
41) Which of the following is involved in regulating pollen tube growth and guidance? [Question ID = 52715]
1. Mg2+ [Option ID = 90852] 2. Co2+ [Option ID = 90855] 3. Ca2+ [Option ID = 90854] 4. Zn2+ [Option ID = 90853]
Correct Answer :- Ca2+ [Option ID = 90854]
42) Which of the following nitrogenous bases of DNA predominantly undergoes methylation? [Question ID = 52722]
1. Cytosine [Option ID = 90883] 2. Thymine [Option ID = 90880] 3. Adenine [Option ID = 90881] 4. Guanine [Option ID = 90882]
Correct Answer :- Cytosine [Option ID = 90883]
43) Which of the following software is not a genome sequence data assembler? [Question ID = 52734]
1. Phrap [Option ID = 90930] 2. Bowtie [Option ID = 90928] 3. Maq [Option ID = 90929] 4. ChipSeeker [Option ID = 90931]
Correct Answer :- ChipSeeker [Option ID = 90931]
44) Which of the molecular probe is used for staining nucleus? [Question ID = 52711]
1. DAPI [Option ID = 90838] 2. Fura-2 [Option ID = 90837] 3. H2DCFDA [Option ID = 90836] 4. Rhodamine 123 [Option ID = 90839]
Correct Answer :- DAPI [Option ID = 90838] 45) Which one of the following species is used for the extraction of Geranium oil? [Question ID = 52727]
1. Cymbopogon flexuosus [Option ID = 90900] 2. Pelargonium graveolens [Option ID = 90901] 3. Schleichera trijuga [Option ID = 90903] 4. Rosa damascena [Option ID = 90902]
Correct Answer :- Pelargonium graveolens [Option ID = 90901]
46) Mutagenesis by ethidium bromide is brought about by [Question ID = 52730]
1. trans-lesion synthesis during replication. [Option ID = 90915] 2. incorrect base-pairing. [Option ID = 90912] 3. indels following insertion. [Option ID = 90913] 4. instability of base-pairing. [Option ID = 90914]
Correct Answer :- trans-lesion synthesis during replication. [Option ID = 90915]
47) In bZIP transcription factors, leucine zipper is involved in [Question ID = 52693]
1. protein-DNA hydrophilic interaction. [Option ID = 90766] 2. protein-DNA hydrophobic interaction. [Option ID = 90767] 3. protein-protein hydrophilic interaction. [Option ID = 90764] 4. protein-protein hydrophobic interaction. [Option ID = 90765]
Correct Answer :- protein-protein hydrophobic interaction. [Option ID = 90765]
48) Homeo box is a [Question ID = 52687]
1. protein-RNA interaction domain. [Option ID = 90742] 2. protein-DNA interaction domain. [Option ID = 90740] 3. protein-protein interaction domain. [Option ID = 90741] 4. DNA-DNA interaction domain. [Option ID = 90743]
Correct Answer :- protein-DNA interaction domain. [Option ID = 90740]
49) Infection by which pathogen leads to hairy root formation in plants? [Question ID = 52706]
1. Agrobactierum rubi [Option ID = 90817] 2. Agrobacterium rhizogenes [Option ID = 90818] 3. Agrobacterium pusense [Option ID = 90819] 4. Agrobacterium tumefaciens [Option ID = 90816]
Correct Answer :- Agrobacterium rhizogenes [Option ID = 90818] 50) Dynamic behavior of protein in lipid bilayer can be studied by [Question ID = 52716]
1. single particle reconstitution. [Option ID = 90859] 2. FLIP and FRAP. [Option ID = 90858] 3. BiFC. [Option ID = 90856] 4. FRET. [Option ID = 90857]
Correct Answer :- FLIP and FRAP. [Option ID = 90858]