SEACFMD Laboratory Network Meeting Lanzhou, China 26-28 September 2017

FMD Monitory on the Borders of Yunnan Province with Laos, Myanmar and Vietnam

P.R. China

Dr. Li Huachun

Yunnan Animal Science and Veterinary Institute Yunnan Tropical and Subtropical Animal Virus Disease Laboratory Kunming, P. R. China Outline

• Background

• FMDV Surveillance

• Laboratory activities Back Ground of Yunnan Border

Yunnan Mongolia Province 辽宁

内蒙古 Kear. 北京 4060 kilometers 山西 boarder with 甘肃 Vietnam, Lao PDR China and Myanmar. 8 prefectures, 25

Yunn counties, 209 an Provi township villages Myanm nce ar along the border. Laos 50 traffic roads, 1 Vietn am. rail way link and more than 1000 Thailand small paths connecting with Myanmar, Laos and Vietnam. Yunnan Tropical and Subtropical Animal Viral Disease Laboratory (YTSAVDL)

• YTSAVDL was established and opened at 1996. – Including 3044 m2 building • BSL-2 1050 m2 • BSL-3 80m2、 • ABSL-3 810m2 (under construction) Cattle，buffalo and pig movement from GMS countries

YunnanKunming Yunnan

Cattle and Buffalo Pig Fattened cattle from non GMS countries by sea 云南、广西活牛流动路径 Cattle，Buffalo and pigs Movement from Yunnan and Guangxi to South China

Kunming

India Nannin

牛 Cattle and Buffalo 猪Pig

FMD Monitory at the Borders of China-Lao’s, China-Myanmar and China-Vietnam

• Borders targeted

– China-Lao’s Border • Mengla County • Jiangcheng County – China-Myanmar Border • Ruili County • Mangshi County • Genma County • Menlian County • Lancang County • Menghai County • Jinghong County – China-Vietnam Border – Funing Count • Animals targeted and specimens collected

– The cattle and buffalo originated from other countries (Sera, OP fluids) – Slaughter house (lymph nodes) – Local pigs and cattle • Laboratory test and data analysis – Antibody test C-ELISA ，NSP-ELISA --- 1 day – FMDV RNA Group test： qRT-PCR 2-3 days Typing：RT-PCR and sequence Serum collection Oesophageal-pharyngeal (OP) liquid collection

Probang (sputum) cup

Probang cup washed with tap water after treated with sodium hydroxide before used for another collection. FMDV RNA Test for lymph nodes collected from pigs, cattle and buffalo at slaughter houses (Yunnan 2016) Animal Number of animal location qRT-PCR RT-PCR virus number test 1-100 pig Menglong, Jinghong 100 0 O SEA /Mya-98 101-200 cattle Funing,Wenshang 100 5 2 O Cathay 201-250 pig Mengla 50 0 251-270 pig Cangyuan 20 0 0 271-280 cattle Cangyuan 10 0 281-290 cattle Zhengkang 10 1 0 290-310 pig Zhengkang 20 0 311-350 pig Gengma 40 0 351-430 cattle Mangshi 80 1 1 O SEA /Mya-98 431-462 pig Mangshi 30 0 463-562 pig Menghai 100 0 563-662 cattle Menglian 100 0 663-762 cattle Jinghong 100 0 Cattle 400 7 Positive 1.75% pig 360 0 Positive 0% total 760 7 Positive 0.9% cattle and buffalo came from neighbor countries FMDV RNA Test for OP fluid collected from imported cattle and buffalo from Lao’s, Thailand and Myanmar (Yunnan 2016)

Number of FMDV Typing by RT-PCT County Number of animal Positive by Real Time PCR O A Asia-1

Mangshi 51 0 0 0 0

Jinghong 31 3 2 1 0

Menghai 32 6 1 0 0

Menglian 42 0 0 0 0

Gengma 30 0 0 0 0

Funing 30 1 0 1 0

Total 216 10 3 2 0

Positive rate / 4.63% 1.39% 0.92% 0% Specimens collected from Jan-Jul 2017

cattle and prefectures county pig lymph nodes OP fluid buffalo sum Mangshi 300 100 57 50 507 Ruili 300 100 30 49 479 Dehong Yingjiang 200 - - / 200 Longchuan 400 100 30 50 580 Jinghong 1200 300 84 60 1644 Xishuangbanna Mengla 400 100 50 60 610 Menghai 400 100 100 60 660 Pu’er Menglian 400 100 103 / 603 Cangyuan 150 100 30 / 280 Lincang Gengma 150 100 40 / 290 Zhengkang 150 100 30 / 280 Wenshan Funing 400 100 60 50 610 Total / 4450 1300 614 379 6,743 FMDV RNA Test for lymph nodes collected from pigs, cattle and buffalo at slaughter houses (Yunnan 2017)

Percent Number of qRT-PCR county animal source of tested positive positive Menglian pig imported 36 1 2.8% Lincang cattle imported 36 0 0% pig imported 36 0 0% Jinghong cattle imported 36 1 2.8% Ruili cattle imported 18 0 0% pig imported 16 1 6% Menghai cattle imported 16 0 0% pig imported 17 0 0% Longchuan pig imported 36 1 2.8% Funing cattle local 36 0 0%

Total 283 4 1.41% FMDV RNA Test for OP fluid collected from imported cattle and buffalo from Lao’s, Thailand, Myanmar and local (Yunnan 2016) qRT-PCR FMDV * Sequence typing Number RT-PCR County Animal source positive tested positive positive O A Asia-1 （%） Mangshi cattle 50 imported 3 6.00% 1 Mya98 Ruili buffalo 49 imported 1 2.04% 0 — Ind2001d Longchuan cattle 50 imported 5 10.00% 2 Mya98 cattle 17 imported 2 11.76% 1 Cathy Jinghong buffalo 43 imported 0 0 0 cattle 30 imported 1 3.33% 0 — Menghai buffalo 30 imported 2 6.67% 1 Ind2001d

Mengla cattle 60 imported 2 3.33% 2 Ind2001d new？

Funing callte 50 local 0 0% 0 — total - 379 16 4.22% 7 O型 A型

ME-SA /IND-2001d KX712091.1 O/BAN/GO/Ka-236(Pig)/2015 Oct 2016, Jinghong KY077626.1 O/BAN/MG/Sa-294/2016 KY399470.1 O/MYA/2496/2015 KY449055.1 O/NEP/7/2016 A22/IRQ/2000 KY077624.1 O/BAN/KU/Fu-283/2016 O/YNJH/CHA/102/2016 A/YNML/1/2015 O/BANGO/Ka-236(Pig)/2015 A22/TB/CHA O/YNLCh/CHA/1720/2017 Ind2001d O/YNMLa/CHA/1908/2017 A22/XJ/CHA/004 O/YNMH/CHA/1816/2017 KC506528.1 O/IND150/2012 A/IRN/2/87 KC506475.1 O/IND149/2010 A/IRN/22/99 KJ825808.1 O/IND141(306)/2013 ME-SA KJ825804.1 O/IND/298(634)/2013 A/IRQ/33/2002 south asia and west asia group KJ825805.1 O/IND/341(707)/2013 O/IRN/20/2004 A/IRQ/17/2000 O/BHU/40/2004 A/TUR/17/2001 O/NEP/6/2003 O/TUR/7/2003 A/Argentina/2001 O/SYR/3/2002 O/AFG/210/2004 A12 valle119/iso20 O/VIT/1/2003 A/Pommern/EGER/47 O/IRQ/30/2000 O/SKR/1/2002 A2/SPA/43 O/JPN/2000 O/SAR/1/2000 A/ETH/7/92 O/CAM/2/98 A/SUD/1/85 O/TAI/2/2000 Afric genetic lineage O/MYA/1/98 A/GAM/44/98 O/TAI/4/99 A/NIG/4/79 O/TAI/3/2003 South-east asia (mya98) O/YNLCh/CHA/1731/2017 A/YNML/CHA/1925/2017 O/YNMS/CHA/1649/2017 O/YNMH/CHA/31/2016 A/MAY/2/2002 O/YNJH/CHA/100/2016 O/KEN/2/95 A/YNFN/CHA/2/2016 O/GHA/5/93 A/MAY/4/2012 O/ALG/1/99 O/CIV/8/99 A/VIT/20/2012 O/JAV/5/72 O/ISA/1/74 A/YNJH/CHA/95/2016 southeast asia group O/ISA/1/62 A/YNKM/CHA/2014 O/ISA/8/83 O/ISA/9/74 KF450794.1 A/GDMM/CHA/2013 O/PHI/12/2004 O/TW/257/2009 A/GDMM/CHA/2013 O/YNJH/CHA/1870/2017 KT968663.1 A/HY/CHA/2013 O/YNML/CHA/2/2015 Cathy O/HKN/16/96 A/HY/CHA/2013 O/TAW/81/97 O/TW/256/2001 0.12 0.10 0.08 0.06 0.04 0.02 0.00 0.12 0.10 0.08 0.06 0.04 0.02 0.00

Test at Jun 2017 Test at Oct 2016 Serological results of FMDV for the cattle and buffalo originated from other countries (Yunnan 2014-2017）

Number Year of O A Asia-1 3ABC tested

2014-2015 3837 1469 38.29% 880 22.93% 776 20.22% 1162 30.28%

2016 2799 817 29.92% 215 7.22% 881 30.70% 905 34.67%

2017 4445 2860 64.34% 2532 56.96% 1781 40.07% / / ADB LOA Project Stake Holder Workshop for Cross Border Animal Movement and Trade ADB LOA Project Training farmers Animal Movement Studies at China-Myanmar’s Border Animal Movement Studies at China-Myanmar’s Border • Laboratory Training for Lao’s, Myanmar and Vietnam in 2015 Laboratory Training for Lao’s, Myanmar and Vietnam in 2017 Field Trip Studies (for Lao’s, Myanmar) in China-Lao Border in 2017 Field Trip Studies (for Lao’s, Myanmar) in China-Myanmar Border in 2017 Thank you!