“Culture of abalone Haliotis asinina in bottom cages with bamboo substrates”

Lota A. Creencia, Adzel Adrian G. Baldevieso, Bjorn H. Lota, Ariel D. Valoroso Western Philippines University College of Fisheries and Aquatic Sciences Puerto Princesa City, Palawan 1 Introduction • Abalone – Herbivorous • Benthic diatoms – larvae and post larvae • Macro algae – juvenile and adult – Inhabit in coral reef area • 8-20 m depth – About 100 species worldwide; 5 found in the Philippines • Haliotis asinina, H. varia, H. ovina, H. glabra, H. planata

2 Distribution of Haliotis asinina (after Poutiers 1998)

3 • Haliotis asinina – Promising aquaculture species » Thailand, Indonesia and Philippines – Land-based or sea-based culture – Optimum parameters » Clean sea water » pH – 8 » Temperature – 26-28oC » Salinity – 32-35ppt

4 Cook & Gordon , 2010

5 6 Culture substrates  PVC pipes  Plastic plates

7 Objectives • This study aimed to 1. Determine the growth of abalone in terms of: – Total weight gain – Shell length increment – Specific growth rate – Shell growth rate 2. Determine the survivorship 3. Determine the food consumption and food conversion efficiency

8 Materials and Methods This study was conducted Experimental site at western Philippines university binduyan – Western Philippines University – Binduyan marine research station it Marine Research Station (WPU-BMRS) is about 79 km away from the city proper. The station has existing abalone hatchery, infront of this hatchery with almost 100 m from the shorline, the experimental cages has been installed.

9 Cage preparation 30cm

20cm

Bamboo slat Cut PVC pipe

10 Substrate preparation

15 cm

20 cm 11 Experimental abalone • 40 pcs juvenile abalone each cage • Initial length of 17-36 mm • feed with Gracilaria firma

12 Distribution of treatments and replicates –Completely Randomized Design (CRD)

T1R1 T2R2 FR3

T2R3 FR2 T2R3

FR2 T1R3 T1R2

T1= Bamboo substrate T2 = No substrate F = Food control 13 Data collection  Every 15 days  SCUBA divers

14 Data Analyses • Weight gain (g) • Specific growth rate • Shell length increment • Shell length growth rate • Consumption rate • Food conversion efficiency

15 Analysis • Microsoft excel • Megastat • Past application –Data = mean ± standard error –T-test –ANOVA

16 Results

17 Growth – Ave. Shell Length

40

35

Bamboo 30 substrate

No substrate

25 Shell legnth (mm) legnth Shell

20 0 day 15 days 30 days 45 days 60 days

Culture period 18 Shell length growth rate 0.16 P<0.05 0.14 0.12

0.10

1 - 0.08 0.06 0.04

0.02 % mm day mm % 0.00 0-15 16-30 31-45 56-60 0-60 Culture period (day) Bamboo No substrate 19 Daily growth rate – shell length

Capinpin & Corre, 1996 – 0.13 mm day-1 The result of this Setyono, 2006 - 0.03 mm day-1 study especially in -1 Setyono, 2007 - 0.04 mm day bamboo substrate is comparable to other NS – 0.12 mm day-1 experiment. BS – 0.15 mm day-1

20 Shell length increment

10.00 P<0.05 9.00 8.00 7.00

6.00 5.00 mm 4.00 3.00 2.00 1.00 0.00 0-15 16-30 31-45 46-60SEAFDEC0-60 2005 – 0.55 mm SLI Culture period (day) Setyono 2006 – 2.26 mm SLI Bamboo No susbtrate 21

Growth - ABW 9 8 7

6 5 4 Bamboo

3 substrate Weight (g) Weight 2 No substrate 1 0 0 day 15 days 30 days 45 days 60 days

Culture period 22 Average specific growth rate

P<0.01 3.00

2.50

1 -

2.00 day

1.50

g

% 1.00

0.50

0.00 0-15 16-30 31-45 46-60 0-60 Culture period (day)

Bamboo No substrate 23 Daily growth rate – Weight To compare the result of this study, although Capinpin & Corre, 1996 – 0.19 gday-1 capinpin and corre, 1996 Setyono, 2006 - 0.04 g day-1 was higher with 0.19 -1 g/day in this Setyono, 2007 - 0.06 g day experiment., setyono 2006 and 2007 was lower BS – 0.08 g day-1 in this experiment especially to compare in -1 NS – 0.05 g day the result of abalone in the bamboo substrate.

24 Weight gain

6.0 P<0.01 5.0

4.0

3.0 grams 2.0

1.0

0.0 0-15 16-30 31-45 46-60 0-60 Culture period (day) Bamboo Substrate No substrate 25 Survivorship

99% 100%

100%

80%

60%

40%

20% Percentage

0% Bamboo substrate No substrate Culture Substrates 26 Food consumed

P>0.05 4000 3500

3000

2500

2000 grams 1500 1000 500 0 0-15 16-30 31-45 46-60 0-60 Bamboo substrate no substrate

Culture period (day) 27 Food conversion efficiency

6 P>0.05 5

4

3 % 2

1

0 0-15 16-30 31-45 46-60 0-60 Culture period (day)

Bamboo substrate no substrate 28 Conclusion

•Bamboo can be utilized as culture substrates for abalone culture in bottom cages with high growth rate

Recommendation

•Long term study is recommended to validate the findings of this study

29 Acknowledgements

DA-BAR

30 THANK YOU

31