Thai Fores Thai Forest Bulletin t Bulletin (Botany) Vol. 47 No. 2, 2019 Vol. t Bulletin (Botany) (Botany) Vol. 47 No. 2, 2019 ISSN 0495-3843 (print) ISSN 2465-423X (electronic) Forest Herbarium Department of National Parks, Wildlife and Plant Conservation Chatuchak, Bangkok 10900 THAILAND http://www.dnp.go.th/botany ISSN 0495-3843 (print) ISSN 2465-423X (electronic) Fores t Herbarium Department of National Parks, Wildlife and Plant Conservation Bangkok, THAILAND THAI FOREST BULLETIN (BOTANY) Thai Forest Bulletin (Botany) Vol. 47 No. 2, 2019 Published by the Forest Herbarium (BKF) CONTENTS Department of National Parks, Wildlife and Plant Conservation Chatuchak, Bangkok 10900, Thailand Page Advisors Wilailux Zumstein & Kamolhathai Wangwasit. Chromosome counts and karyotype reports Chamlong Phengklai, Kongkanda Chayamarit & Thannarin Na Nakorn from Fimbristylis (Cyperaceae) in Thailand 123–132 Ai Nagahama, Shuichiro Tagane, Phetlasy Souladeth, Anousone Sengthong & Tetsukazu Editors Yahara. Gentiana bolavenensis (Gentianaceae), a new species from Dong Hua Sao Rachun Pooma & Timothy Utteridge National Protected Area in southern Laos 133–136 Managing Editor Assistant Managing Editor Shuichiro Tagane, Sukid Ruengruea, Hidetoshi Nagamasu & Somran Suddee. Two new species and one new record of Dichapetalum (Dichapetalaceae) in Thailand 137–144 Nannapat Pattharahirantricin Sawita Yooprasert Joeri S. Strijk & Hoang Thanh Son. Lithocarpus gigantophyllus (Fagaceae), a new record Editorial Board from Loei province (Thailand) 145–151 Rachun Pooma (Forest Herbarium, Thailand), Timothy Utteridge (Royal Botanic Gardens, Kew, UK), Wirata Rujichaipimon, Henrik Æ. Pedersen, Orporn Phueakkhlai, Somran Suddee, David A. Simpson (Royal Botanic Gardens, Kew, UK), John A.N. Parnell (Trinity College Dublin, Sarawood Sungkaew & Paweena Traiperm. On scientific requirements for presentation Ireland), David J. Middleton (Singapore Botanic Gardens, Singapore), Peter C. van Welzen (Naturalis Bio- of “new records”: the case of Dendrobium ruckeri (Orchidaceae) 152–158 diversity Center, The Netherlands), Hans-Joachim Esser (Botanische Staatssammlung München, Germany), André Schuiteman (Royal Botanic Gardens, Kew, UK), Anders S. Barfod Pranom Chantaranothai. The genus Prismatomeris (Rubiaceae) in Thailand 159–170 (Aarhus University, Denmark), Piyakaset Suksathan (Queen Sirikit Botanic Garden, Thailand), Sawai Mattapha, Felix Forest, Julie Hawkins, Somran Suddee, Naiyana Tetsana & Pimwadee Pornpongrungrueng (Khon Kaen University, Thailand), Stuart Lindsay Pranom Chantaranothai. Three new species, lectotypifications and synonymisations in (Singapore Botanic Gardens, Singapore), Prachaya Srisanga (Queen Sirikit Botanic Garden, Thailand) Millettia (Fabaceae: Faboideae) for Thailand 171–183 Pranom Chantaranothai. A new combination and typifications inMorinda (Rubiaceae) for Thai Forest Bulletin (Botany) (TFB) publishes papers on plant taxonomy (especially of vascular plants), the Flora of Thailand 184–186 nomenclature, phylogeny, systematics, plant geography, and floristics, and in morphology, palynology, cytotaxonomy, chemotaxonomy, anatomy and other relevant disciplines. Priority is given to papers written Woranart Thammarong. Heterostemma succosum (Apocynaceae), a new record for Laos 187–189 by staff of the Forest Herbarium and by botanists working on the Flora of Thailand Project. Limited space is Michele Rodda. The identity of Periploca cordata (Apocynaceae) 190–192 available for other relevant papers. Wittaya Kaewsri & Supanath Kanjanawattanawong. Amomum spathilabium (Zingiberaceae: The journal now uses Thai Journal Online (ThaiJO) for online submission and peer review at Alpinieae), a new species from northern Thailand 193–195 www.tci-thaijo.org/index.php/ThaiForestBulletin. Manuscripts are considered on the understanding that Hubert Kurzweil & Paul Ormerod. A review of the Calanthe group (Orchidaceae) in Myanmar 196–225 their contents have not appeared, or will not appear, elsewhere in the same or abbreviated form. Before Somran Suddee, Alan J. Paton, John A. N. Parnell, Pachok Puudjaa, Wittawat Kiewbang submitting a manuscript please read the Guidelines for authors. These guidelines must be followed & Sukid Rueangruea. Five new species of Platostoma (Lamiaceae) from North-Eastern precisely otherwise publication of the manuscript will be delayed. In addition, papers published online Thailand 226–240 will be distributed simultaneously in printed form to several libraries, and bound hard copy volumes will appear later. Exchange with botanical journals or periodicals pertaining to plant taxonomy would be Thiamhathai Choopan, Paul J. Grote, Kongkanda Chayamarit & David A. Simpson. appreciated. A checklist of Acanthaceae subfamily Nelsonioideae in Thailand 241–259 Thitiporn Pingyot, Piyakaset Suksathan & Nawin Inthakul. Bulbophyllum seidenfadenii FOREST HERBARIUM (Orchidaceae), a new record for Thailand 260–263 Director: Rachun Pooma David M. Johnson & Nancy A. Murray. Two new species of Xylopia (Annonaceae) from Curator: Nannapat Pattharahirantricin peninsular Thailand 264–269 BKF Staff: Somran Suddee, Piyachart Trisarasri, Voradol Chamchumroon, Somchai Prasertthaicharoen, Nanthawan Suphuntee, Preecha Karaket, Thanongsak Jonganurak, Pachok Puudjaa, Tarathorn Kaewplub, Narong Koonkhunthod, Montri Saengsawasti, Naiyana Tetsana, Sukontip Sirimongkol, Manop Poopath, Sommanussa Tanikkool, Sukid Rueangruea, Sawita Yooprasert, Saksan Kaitongsuk, Theerawat Thananthaisong, Orathai Kerdkaew. Front Cover: Xylopia spp. Printed at: Prachachon Co., Ltd. 35 Soi Pipat, Silom Road, Bangrak, Bangkok 10500, Thailand Tel : 0 2636 6550 THAI FOREST BULL., BOT. 47(2): 123–132. 2019. DOI https://doi.org/10.20531/tfb.2019.47.2.01 Chromosome counts and karyotype reports from Fimbristylis (Cyperaceae) in Thailand WILAILUX ZUMSTEIN1 & KAMOLHATHAI WANGWASIT1,* ABSTRACT The somatic chromosome numbers and karyotype of Thai Fimbristylis species were determined from root tips. The chromosome counts and karyotype formulae are 2n=20 (16m+4sm) for F. argentea, 2n=26 (2M+10m+14sm) for F. dichotoma, 2n=4 (2m+2sm) for F. pubisquama, 2n=10 (6m+4sm) for F. insignis, 2n=10 (8m+2sm) for F. eragrostis, F. fusca, F. littoralis, F. ovata and F. umbellaris, and 2n=10 (4m+6sm) for F. thomsonii. Chromosome numbers of F. eragrostis, F. fusca, F. insignis, F. pubisquama and F. thomsonii are newly reported. The present study determined several karyotype asymmetry values. In addition, correlation between the karyotype asymmetry values were shown in this study. The dispersion diagram was found based on A1 and A2. The Stebbins categories of Fimbristylis investigated taxa were 3A, 4A and 4B. KEYWORDS: Chromosome number, Cyperaceae, Fimbristylis, karyotype. Accepted for publication: 6 June 2019. Published online: 11 July 2019 INTRODUCTION no visible primary constrictions. The cytological data for Thai Fimbristylis species are comparatively Fimbristylis Vahl is a large genus of Cyperaceae few, with 16 reported covering only 26% of the Thai belonging to tribe Abildgaardieae. The genus comprises taxa (Tanaka, 1939; Sharma & Bal, 1956; Hsu, 1972; about 382 species worldwide (Govaerts et al., 2019) Sanyal & Sharma, 1972; Rath & Patnaik, 1974; and is distributed mainly in the tropical and subtropics, Nijalingappa, 1975a; 1975b; Subramanian, 1988; with the greatest number in Southeast Asia and Roalson, 2008; Saensouk & Saensouk, 2018). Thus, Northeastern Australia. It consists of annual and the aim of the present investigation is to report the perennial plants. There are 63 species of Fimbristylis chromosome number and to analyze karyotype in Thailand, of which three are recently reported characteristics of Thai Fimbristylis. (Simpson & Koyama, 1998; Maxwell, 2002; Wangwasit et al., 2012; Wangwasit et al., 2017). The chromosome numbers of 63 Fimbristylis species MATERIAL AND METHODS have been reported (Tanaka, 1939; Sharma & Bal, Cytological observations were conducted on 1956; Sanyal & Sharma, 1972; Hsu, 1972; Rath & ten Thai Fimbristylis species, eight of them have Patnaik, 1974; Nijalingappa, 1975a; 1975b; 1977; never been examined before. Voucher specimens Subramanian, 1988; Bir et al., 1992; Yano & were deposited in Mahasarakham University Hoshino, 2006b; Roalson, 2008; Uchiyama et al., Herbarium (MSUT) (Table 1). Somatic chromosomes 2010). The chromosome count varied from 2n=6 in were observed from root-tip meristematic cells. The F. umbellaris (Lam.) Vahl (Rath & Patnaik, 1977) root tips were fixed in a solution of acetic acid and to 2n=52 in F. cymosa R.Br. (Yano & Hoshino, ethyl alcohol (1:3) for 24 hours and stored in 70% 2006b). The chromosome size varied from 1.5 to alcohol under refrigeration. The samples were above 3 µm (Bir et al., 1986). Hoshino et al. (2000) squashed in 2% acetocarmine. Chromosome numbers reported that the chromosomes of Cyperaceae were determined from ten cells under a light possessed non-localized centromeres and there are 1 Department of Biology, Faculty of Science, Mahasarakham University, Mahasarakham 44150, Thailand. * Corresponding author: [email protected] © 2019 Forest Herbarium 124 THAI FOREST BULLETIN (BOTANY) VOL. 47 NO. 2 microscope (Zeiss series model Axiostar plus). The Section Abildgaardia best cells were photographed at 1000x magnification. Fimbristylis ovata (Burm.f.) J.Kern Karyotype formula was determined based on The somatic chromosome number is 2n=10, centromere position (Levan et al., 1964). The relative which is congruent with Nijalingappa (1975b). length (RL),