Human Head and Neck Cancer Cell Lines CLS Cell Lines Service Part of the CLS Cell Bank

Human Head and Neck Cancer Cell lines CLS Cell Lines Service Part of the CLS cell bank

Table 1: Human Head and Neck cancer cell lines: Origin and General Characteristics

Organism, Tissue / Primary Name of cell line Cell type Age / Gender Morphology Growth properties CLS order no. Ethnicity tumor

Submaxillary gland Human submaxillary Homo sapiens (submandibular 63 / A-64 CLS 1 gland Adenoma cell (Human) / gland; lt. glandula Monolayer, adherent 300199 Male line Caucasian submandibularis), Adenomacarcinom Homo sapiens Human Thyroidea Anaplastic thyroid Anaplastic thyroid C643 2 (Human) / Male Epithelial 300298 Carcinoma cell line carcinoma carcinoma Caucasian Homo sapiens Humane HNSCC Mouth, Squamous CLS-354 3 (Human) / 51 / Male Epithelial Monolayer, adherent 300152 (Mund) Zelllinie epithelial carcinoma Caucasian Human Oesophagus Homo sapiens Esophageal COLO-680N 4 Squamous cell (Human) / 57 / Female squamous cell Epithelial Monolayer, adherent 300464 carcinoma cell line Caucasian carcinoma Pharynx; from Human Pharynx Homo sapiens metastatic site: Detroit-562 5 Carcinoma, epithelial (Human) / Adult / Female Epithelial Monolayer, adherent 300399 pleural effusion, cell line Caucasian Carcinoma Human Larynx Homo sapiens HEp-2 (HeLa Larynx, Epidermoid Carcinoma, (Human) / Female Epithelial Monolayer, adherent 300397 derivative) 6 carcinoma epidermoid cell line Caucasian Larynx, Head and Homo sapiens Human HNSCC neck squamous cell HNO210 9, 10 (Human) / 69 / Male Epithelial Monolayer, adherent 300134 (Larynx) cell line carcinoma (HNSCC) Caucasian of the oral cavity Tongue, Head and Homo sapiens Human HNSCC neck squamous cell HNO223 10 (Human) / Epithelial Monolayer, adherent 300142 (Tongue) cell line Male carcinoma (HNSCC) Caucasian of the oral cavity Head and neck Homo sapiens Human HNSCC 62 / squamous cell HNO258 8 (Human) / Epithelial Monolayer, adherent 300146 (Oral cavity) cell line Male carcinoma (HNSCC) Caucasian of the oral cavity Human HNSCC Homo sapiens 52 / Head and neck HNO41 9 (Oropharynx; Tonsil) (Human) / Epithelial Monolayer, adherent 300126 Male squamous cell cell line Caucasian

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Organism, Tissue / Primary Name of cell line Cell type Age / Gender Morphology Growth properties CLS order no. Ethnicity tumor

carcinoma (HNSCC) of the oral cavity

Tongue, Head and Human HNSCC Homo sapiens neck squamous cell HNO97 10 (Oral cavitiy; (Human) / Epithelial Monolayer, adherent 300129 Female carcinoma (HNSCC) Tongue) cell line Caucasian of the oral cavity Nasal septum Human Nasal Homo sapiens (pleural effusion), RPMI 2650 11 squamous (Human) / Male Epithelial Monolayer, adherent 300323 Squamous cell carcinoma cell line Caucasian carcinoma Homo sapiens Human Thyroidea Anaplastic thyroid SW-1736 12 (Human) / Female Epithelial Monolayer, adherent 300453 carcinoma cell line carcinoma Caucasian Human HNSCC Homo sapiens (Thyroidea) 59 / Thyroidal Squamous SW-579 13 (Human) / Epithelial Monolayer, adherent 300346 squamous cell Male cell carcinoma Caucasian carcinoma cell line Human Retina Homo sapiens 2,5 / Cluster in Y-79 14 Retinoblastoma cell (Human) / Retinoblastoma Multicellular clusters 300382 Female Suspension line Caucasian

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Table 2: Human Head and Neck cancer cell lines: Special Features

Tissue / Primary Name of cell line Cell type Organism, Ethnicity Age / Gender Morphology Growth properties CLS order no. tumor

Human submaxillary A-64 CLS 1 gland Adenoma cell RRID:CVLL_5968 300199 line

Human Thyroidea C643 2 RRID:CVCL_5969 300298 Carcinoma cell line

Keratin Humane HNSCC Reverse CLS-354 3 RRID:CVCL_5971 300152 (Mund) Zelllinie Transcriptase: neg

Cells express BMP-6 Human Oesophagus (bone morphogenetic COLO-680N 4 Squamous cell protein) in standard RRID:CVCL_1131 300464 carcinoma cell line cell cultivation conditions. Keratin Human Pharynx Reverse Detroit-562 5 Carcinoma, epithelial G6PD, B RRID:CVCL_1171 300399 Transcriptase: neg cell line p53 positive Keratin Human Larynx HEp-2 (HeLa Reverse Carcinoma, G6PD, A RRID:CVCL_1906 300397 derivative) 6 Transcriptase: neg epidermoid cell line

Human HNSCC HNO210 9, 10 RRID:CVCL_D215 300134 (Larynx) cell line

Human HNSCC HNO223 10 RRID:CVCL_D219 300142 (Tongue) cell line

Human HNSCC (Oral HNO258 8 RRID:CVCL_D221 300146 cavity) cell line

Human HNSCC HNO41 9 (Oropharynx; Tonsil) RRID:CVCL_D224 300126 cell line

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Tissue / Primary Name of cell line Cell type Organism, Ethnicity Age / Gender Morphology Growth properties CLS order no. tumor

Human HNSCC (Oral HNO97 10 cavitiy; Tongue) cell RRID:CVCL_D227 300129 line Mucoid; keratin Human Nasal Reverse RPMI 2650 11 squamous carcinoma G6PD, B RRID:CVCL_1646 300323 Transcriptase: neg cell line

Human Thyroidea V600E type BRAF SW-1736 12 RRID:CVCL_3883 300453 carcinoma cell line Mutation

Me-2, 1-2; PGM3, 1; PGM1, 1-2; ES-D, 1; Human Thyroidea AK-1, 1; GLO-1, 2; Antigen Expression: SW-579 13 squamous cell RRID:CVCL_3603 300346 G6PD, B; Phenotype blood type O; Rh+ carcinoma cell line Frequency Product: 0.0209 PGM1, 1; G6PD, B; Hypertriploid, with Human Retina ES-D, 1; AK-1, 1; abnormalities including Y-79 14 Retinoblastoma cell GLO-1, 2; Phenotype dicentrics, breaks, RRID:CVCL_1893 300382 line Frequency Product: pulverizations and 0.1373 minutes

Information on cell culture conditions, authentication data and others can be found on the website: www.clsgmbh.de

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References:

1. Reinartz, M. (2016) Proteomische Funktionsanalyse humanbiologischer Systeme mittels chemisch modifizierter Proteine. Dissertation, Rheinische Friedrich-Wilhelms-Universität Bonn. 2. Mark, J., Ekedahl, C., Dahlenfors, R., and Westermark, B. (1987) Cytogenetical observations in five human anaplastic thyroid carcinomas. Hereditas Volume 107 (2): 163-174. 3. Kubler, A.C., Reuther, T., Staff, C., Haase, T., Flechtenmacher, C., Benner, A., Scheer, M., Zillmann, U. (2001) Clinical effectiveness of m-THPC-PEG in a new xenogenic animal tumor model for human squamous epithelial carcinomas.(Article in German). Mund Kiefer Gesichtschir 5(2):105-13. 4. Raida, M., Sarbia, M., Clement, J.H., Adam, S., Gabbert, H.E., Hoffken, K. (1999) Expression, regulation and clinical significance of bone morphogenetic protein 6 in esophageal squamous-cell carcinoma. Int J Cancer. 83(1):38-44. 5. Peterson, W.D. Jr, Stulberg, C.S., Swanborg, N.K., Robinson, A.R. (1968) Glucose-6-phosphate dehydrogenase isoenzymes in human cell cultures determined by sucrose-agar gel and cellulose acetate zymograms. Proc. Soc. Exp. Biol. Med. 128: 772-776. 6. Ogura H, Yoshinouchi M, Kudo T, Imura M, Fujiwara T, Yabe Y. (1993) Human papillomavirus type 18 DNA in so-called HEP-2, KB and FL cells--further evidence that these cells are HeLa cell derivatives. Cell Mol Biol (Noisy-le-grand) 39(5):463-7. 7. Vasvari, G.P., Dyckhoff, G., Kashfi, F., Lemke, B., Helmke, B.M., Schirrmacher, V., Plinkert, P.K., Beckhove, P., Herold-Mende, C. (2007) Combination of thalidomide and cisplatin in an HNSCC model results in an enhanced anti-angiogenic activity in vitro and in vivo. Int J Cancer 121:1697-704. 8. Nothelfer, E., Zitzmann-Kolbe, S., Garcia-Boy, R., Krämer, S., Herold-Mende, C., Altmann, A., Eisenhut, M., Mier, W. and Haberkorn, U. (2009) Identification and Characterization of a Peptide with Affinity to Head and Neck Cancer. The Journal of Nuclear Medicine 50(3):426-434. 9. Vasvari, G.P., Dyckhoff, G., Kashfi, F., Lemke, B., Helmke, B.M., Schirrmacher, V., Plinkert, P.K., Beckhove, P., Herold-Mende, C. (2007) Combination of thalidomide and cisplatin in an HNSCC model results in an enhanced anti-angiogenic activity in vitro and in vivo. Int J Cancer 121:1697-704. 10. Nothelfer, E., Zitzmann-Kolbe, S., Garcia-Boy, R., Krämer, S., Herold-Mende, C., Altmann, A., Eisenhut, M., Mier, W. and Haberkorn, U. (2009) Identification and Characterization of a Peptide with Affinity to Head and Neck Cancer. The Journal of Nuclear Medicine 50(3):426-434. 11. Moorhead, P.S. (1965) Human tumor cell line with a quasi-diploid karyotype. Exp Cell Res 39: 190-6. 12. Heldin, N.E., Gustavsson, B., Claesson-Welsh, L., Hammacher, A., Mark, J., Heldin, C.H., Westermark, B. (1988) Aberrant expression of receptors for platelet-derived growth factor in an anaplastic thyroid carcinoma cell line. Proc Natl Acad Sci USA 85: 9302-6. 13. Fogh, J., Wright, W.C., Loveless, J.D. (1977) Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst 58: 209-14. 14. Reid, T.W., Albert, D.M., Rabson, A.S., Russell, P., Craft. J., Chu, E.W., Tralka, T.S., Wilcox, J.L. (1974) Characteristics of an established cell line of retinoblastoma. J Natl Cancer Inst 53: 347-360. 15. http://web.expasy.org/cellosaurus/ - the cellosaurus represents a detailed data collection bank of a plethora of cell line relevant data from various cell banks.

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